id
stringlengths
30
30
corpus
stringclasses
3 values
sentence
stringlengths
10
1.38k
token
stringlengths
1
16
complexity
float64
0
0.86
difficulty
stringclasses
5 values
3D5G8J4N5A4UAGA7UUQG0CCYL34TV2
biomed
These findings are in complete agreement with earlier studies that utilized rats and fairly selective D2R antagonists [3-8], as well as even very extensive 6-OHDA lesions [1,10,11] in associative and operant learning paradigms.
paradigms
0.475
neutral
307L9TDWJYSC3U34DOYY8IEN52MN3X
biomed
Reversal learning in rodents can serve as an index of learning adaptability and alertness that correlates with human attentional-shift paradigms [17,18].
paradigms
0.5
neutral
3HRWUH63QU2FH9Q8R7MRNFC7JXLN5T
biomed
The 1615del10 mutation also removes the last 5 amino acids of the C-terminal peptide [28].
amino
0.375
easy
3IQ9O0AYW6ZPOAQ7VNRXLNM4D26TI5
biomed
In light of these newer findings, our intermediate MSUD mouse provides a model to test the effect of thiamin with respect to BCKDH activity and blood amino acid levels at the level of the whole animal.
amino
0.407895
neutral
3VW0145YLYCH6QKT48BLBA6CW86JMB
biomed
The μ-80 k and m-80 k subunits share 62% amino acid sequence identity, and are very similar in terms of structure, protein chemistry, and in vitro substrate specificity.
amino
0.433333
neutral
3L60IFZKF3IZN6OSPBSN7SI12UYHHQ
biomed
The repressive activity of mr-s is due to its C-terminal region (amino acid 463 to 542).
amino
0.455882
neutral
379OL9DBSSESUVWY1Z8JGBFG9D3Y9G
biomed
CAPAN-1 cells carry a deletion of BRCA2 on one homolog and codes for a protein truncated at amino acid 1981 on the other homolog.
amino
0.482143
neutral
35YHTYFL1G3KRBS6ZALRJ8XXUUWVFW
biomed
After initial screening of founders, we identified four lines of tet-APPswe/ind mice that produced very high levels of transgene product in offspring coexpressing tTA (Figures 1 and Figure S1).
founders
0.375
easy
3FK4G712NX0D3OGOBZGLFKW5KHTSS6
biomed
It is somewhat puzzling that 22 of the 24 Tas2rs examined encode variant proteins in B6 and D2 mice even though these strains exhibit similar taste responses to bitter compounds such as DB or cyclohexamide [47].
compounds
0.375
easy
3XT3KXP24ZYJMCFVZAJ4ADUQTTXI6J
biomed
Annexin A7, the first annexin to be described, was isolated as the agent that mediated aggregation of chromaffin granules and fusion of membranes and phospholipids in the presence of Ca2+-ions [3].
granules
0.375
easy
3HKIF5DF6YXS84BY7TMD9B8C4HJ9GZ
biomed
At the cellular level annexin A7 can be detected in the cytosol, at the plasma membrane, around the nucleus, at vesicular structures including adrenal chromaffin granules, and at the t-tubule system [5,6].
granules
0.426471
neutral
34YWR3PJ28ALYWIBW73O4M7E44D0X0
biomed
Finally, movement of pigment granules within melanocytes or from melanocytes to keratinocytes makes use of cellular machinery that is shared by a variety of cell types, but that can vary in different regions of the body (reviewed in Marks and Seabra 2001).
granules
0.5
neutral
3O71U79SRBPA5T3JR2KZ3BXZSRJSM0
biomed
Neurotrophin-3 (NT-3) is a key requirement for the development of proprioceptive inputs to motor neurons (Chen and Frank 1999; Chen et al. 2003).
Frank
0.381579
easy
3D1TUISJWI04ON5WVSH7QJ86WFFIUL
biomed
We have previously reported the expression of Annexin A7 in human temporal neocortex and hippocampus obtained from neurosurgery for therapy-refractory epilepsy and found the two Annexin A7 isoforms restricted to the cytoplasm and nuclei of astrocytes, whereas neurons lacked any signal [19].
cytoplasm
0.381579
easy
3OKP4QVBP2X6DNL3ZZ0D56JWAZ0GAK
biomed
It is possible that RAD51 may alternatively be capable of BRCA2-independent transport, but mislocalization of BRCA2 to the cytoplasm may sequester RAD51 and block its normal mode of transport.
cytoplasm
0.431818
neutral
3YO4AH2FPDK1PZHZAT8WAEBL70XQ0Y
biomed
This synapse is characterized by the presence of a ribbon in the presynaptic cytoplasm.
cytoplasm
0.5
neutral
3R4QIDVOJPCBJM6O99CB1MOBIZCEER
biomed
Galectin-3 is a multifunctional oncogenic protein with an anti-apoptotic activity found in the extracellular space, in the nucleus and cytoplasm and in mitochondria.
cytoplasm
0.525
neutral
3XBYQ44Z6P47P5ACK4VCMEVCSC6TWC
biomed
It had been shown previously that the small amount of truncated BRCA2 protein expressed by CAPAN-1 cells was mislocalized to the cytoplasm, consistent with the fact that the truncation protein lacks a nuclear localization signal [28].
cytoplasm
0.625
difficult
36BTXXLZ2V8I0OE069KYR7B3KYFR4G
biomed
While the heterozygous knockout mice for Apc develop adenomatous polyps predominantly in small intestine, the homozygous embryos die before gastrulation.
polyps
0.382353
easy
3G57RS03HH5JSN1F5OO7VR9982V52L
biomed
The results obtained by immunofluorescence were confirmed by extraction of Annexin A7 from nuclei of neuronal and glial cell lines.
nuclei
0.382353
easy
3NZ1E5QA6Z1DG01BOHHIWKCD2825BH
biomed
These data evidence a key role for p150CAF-1 during pre-implantation development, and reveal that this protein is required for the proper 3-D organization of heterochromatin within embryonic cell nuclei.
nuclei
0.4
neutral
3KA7IJSNW65H9AJU8WQWUQHUT83PBN
biomed
In the adult brain Annexin A7 generally was detected in nuclei of neurons.
nuclei
0.425
neutral
3UDTAB6HH6ZVX00DTRXAOJLWX1M09H
biomed
A role of Annexin A7 as well as of other annexins in nuclei remains speculative.
nuclei
0.45
neutral
38RHULDV9YFHGRU1QSW887T3HVVWI3
biomed
The developing gray matter of the rat embryonic spinal cord exhibited primarily nuclear localization of Annexin A11, while immunoreactivity was lost from the nuclei in the adult spinal cord [26].
nuclei
0.466667
neutral
3UDTAB6HH6ZVX00DTRXAOJLWX0P09I
biomed
Arterial occlusion time, tail bleeding and rebleeding time were evaluated as potential predictors of thrombotic response.
occlusion
0.382353
easy
3R868ACW4SC7EVTBBRGCMZA5JK1ZGY
biomed
The genes may interact with components in the Plg network and modulate arterial occlusion time.
occlusion
0.4375
neutral
3CZH926SICETRK9VK30YS0CK59KE4L
biomed
It is not likely that merely the concentration of these components explains the increased rebleeding time and reduced arterial occlusion time in the A/J mice.
occlusion
0.470588
neutral
37M4O367VJI9ZR58F67RA0N7E765M6
biomed
In addition, in the CSS, expression of the A/J phenotypes, rebleeding time and arterial occlusion, was modified in the B6 background, and suggested that interactions occurred among the A/J QTL.
occlusion
0.583333
neutral
37VE3DA4YUHPWTL6PJIRLHF37HYBH9
biomed
Further investigation, beyond the scope of this study would be necessary to determine the contribution in the differences in size and sheer stress of the carotids to arterial occlusion rates in the B6 and A/J mice.
occlusion
0.597826
neutral
3XDSWAMB22SLJRCFM91G5FDMGPUQCR
biomed
Extracorporeal membrane oxygenation (ECMO) is used in some centers to provide an extended period of cardiopulmonary bypass [5,6], while other centers have success using other ventilatory support techniques [7].
oxygenation
0.383333
easy
386T3MLZLNVVJVH3OZRWONQLDUC08Y
biomed
Furthermore, with the growing amount of large-scale proteomic and genomic data becoming publicly available, this analysis can now be extended to reveal the complex interplay between evolution of gene structure and protein function.
interplay
0.383333
easy
3NSM4HLQNRUPDSMYRR2BPK23K4QQQR
biomed
Thus, Dmrt7 likely arose, presumably by duplication and divergence of another Dmrt gene, shortly before or coincident with the mammalian radiation.
divergence
0.383333
easy
3ZZAYRN1I6RZKW1ATI425KIQPACOT0
biomed
Spiral prominence in Slc26a4-/- mice was less prominent, spiral ligament thinner and spiral limbus flatter (Fig. 5).
flatter
0.384615
easy
38VTL6WC4AD9T8UMY9V24VAENHKY5P
biomed
Regulators of sexual differentiation have been identified in a number of organisms, but in contrast to many other developmental processes, such as axial patterning or development of many body parts, the molecular mechanisms that regulate sexual differentiation are highly variable between phyla.
Regulators
0.384615
easy
3QGTX7BCHP29YY4RUVXLI2JGTBP5ZO
biomed
SAM domains (also known as Pointed, HLH, or SPM domains) are ~70 residues long and have been reported as common protein-protein interaction modules [15-17].
Pointed
0.386364
easy
3V8JSVE8YYQ9PRNF9AO58PGR0AOYEV
biomed
Plaques were limited to the forebrain, including the cortex, hippocampus, olfactory bulb, and striatum, where the CaMKIIα promoter is known to be most active [16,28] (Figure S2).
Plaques
0.388889
easy
3G9UA71JVVUYLND6029WSS9M1K4J7V
biomed
These options were narrowed down by the subsequent observation that Capn1-/- mice, which lack the μ-calpain catalytic subunit, were healthy and fertile, although platelet aggregation and clot retraction defects were observed [33].
platelet
0.388889
easy
3RZS0FBRWKA0JFB0MWMR5ANCBT3CPH
biomed
Clot formation and lysis, which ultimately determine thrombosis, requires platelet aggregation, coagulation, and fibrinolytic functions.
platelet
0.390625
easy
35U0MRQMUJ7EXO5ZYD4H626BMVNVOI
biomed
We have also observed a defect in platelet function in the annexin A7 mutant.
platelet
0.444444
neutral
3RQVKZ7ZRKJYFRFYTH34TBN5Z8K27R
biomed
The tail-bleeding assay has been used extensively in mice to assess the impact of deficiencies and over expression of platelet and coagulation proteins in mice.
platelet
0.553571
neutral
3KTZHH2ONIFSM9OAD4EUMKXYGXCM8U
biomed
The μ and m designations derive from the levels of Ca2+ required in vitro for optimal activation; 10–50 μM Ca2+ for μ-calpain and 0.3–0.35 mM Ca2+ for m-calpain.
designations
0.390625
easy
3L4YG5VW9NS1ZAEOHJQ7PMO5IAHDD9
biomed
We suggest that the interaction of PAX6 with HOMER3 and DNCL1 is a mechanism by which synaptic signalling could lead to regulated changes in gene expression in neurons.
signalling
0.390625
easy
3XABXM4AJ15J7OM25XLB3TH3QY9Q8L
biomed
We assume that these ADAMs might contribute to cell migration, differentiation and survival or synaptic regulation by binding to integrins, cell-surface proteins or intracellular signalling molecules [20,21].
signalling
0.413043
neutral
3W31J70BASWZ8OEK94HJ9T1DHJXCKG
biomed
These findings support the idea that FGF9 acts as the antagonist of Wnt4.
antagonist
0.390625
easy
3UEBBGULPFOKEW36UKBMNMVEGNSFUW
biomed
Despite a growing literature demonstrating disrupted associative learning following acute manipulations of dopamine D1R signaling during acquisition of associative learning tasks with food [e.g. [6,7]], the actual contribution of D2R-mediated signaling on acquisition and maintenance of associative learning in rodents is unresolved.
manipulations
0.390625
easy
3TFJJUELSHP4R8AUKYBF9XFJ0JH2CQ
biomed
In various manipulations of the neurotrophins it has been noted that molecular markers for proprioceptive axons such as PV or calcitonin gene-related peptide for nerve growth factor–responsive axons are compromised (Ringstedt et al. 1997; Patel et al. 2000), thus PV immunostaining in Bax/NT-3 double KOs cannot reveal the extent of proprioceptive axons in the periphery.
manipulations
0.45
neutral
32ZCLEW0BZKNRTH6WO3DSE071DCJP2
biomed
Nutritional problems can persist even with adequate oral enzyme supplementation [9] and neutralization of gastric acid to improve lipase function [10], and may involve both impaired digestion and absorption of nutrients [11].
neutralization
0.392857
easy
3J5XXLQDHMBPBZCDFFKPIZ80R4R3VV
biomed
Sequencing of Aqp2 coding region of affected mice identified a thymine to guanine (T to G) transversion (Figure 1A), which is predicted to lead to a valine for phenylalanine substitution at amino acid 204 of the protein (F204V).
Sequencing
0.392857
easy
3VCK0Q0PO5EGM247U1GF6KGG2Q3N03
biomed
However, strategies to prevent thrombosis have lagged far behind due, in part, to the contribution of multiple, and as yet undefined, genetic factors that lead to thrombotic risk.
thrombosis
0.392857
easy
31ANT7FQN82N7D4XO9REIVFBXLCH5E
biomed
The marked independent differences demonstrated in the B6 and A/J mice can be exploited to identify genetic determinants of thrombosis and haemostasis.
thrombosis
0.5
neutral
3WA2XVDZEMH767D93RTDPYWY10W6E4
biomed
The FeCl3-induced model of vascular injury and thrombosis in mice is now widely used to evaluate genetic and pharmacological interventions [24].
thrombosis
0.552083
neutral
3JHB4BPSFK9JLT5CAPRP3PCEA4M9QO
biomed
Human DMRT1 maps to an autosomal locus, which, when hemizygous, is associated with defective testicular development and consequent XY feminization [30].
feminization
0.392857
easy
3KL228NDMVMCP0UPBQD2ZKMSFCNKGY
biomed
WT = wild-type
WT
0.392857
easy
3M93N4X8HKNK4LXYO577NJ5IOYGSJQ
biomed
This response is seen in WT, Bax null and in p75 null DRG explants, and in the absence of any other neurotrophins or target-derived axon guidance molecules.
WT
0.558824
neutral
39WSF6KUV2LXVDJD3NF76YIPM0POEA
biomed
This will normally lead to a temporary remission of the disease in both mice and humans, followed by an exacerbation phase after delivery [7,10,11].
exacerbation
0.397059
easy
3ZXV7Q5FJBOOS2M68TQMTG989NGFCH
biomed
A gene-specific inhibitor in sample 147 prevented amplification of E2F1.
amplification
0.397059
easy
32PT7WK7DMF3HICROUKVCD2TRXSD3B
biomed
However, in each such case, the PCR amplification was efficient enough to enable quantification.
amplification
0.4375
neutral
37SDSEDIN92VQK2LKIVW2S9VIMI182
biomed
APC also interacts with a multitude of other cellular proteins, including axin-2 (AXIN2), plakoglobin (JUP), Asef (ARHGEF4), kinesin superfamily–associated protein 3 (KIFAP3), EB1 (MAPRE1), microtubules, and the human homolog of Drosophila discs large (DLG1).
discs
0.397727
easy
3T5ZXGO9DEOYRKNPENLOGDE7P7BQZL
biomed
At their apex, these neurons have a membranous outer segment, which contains proteins involved in the phototransduction cascade.
apex
0.4
neutral
389A2A304OIXVY7G5B71Q9M43LRC0A
biomed
In summary, our findings demonstrate the impact of epistatic interactions between 129 and C57BL/6 genomes on the development of SLE and illustrate how these background gene effects may lead to incorrect interpretations when analysing the autoimmune phenotype of specific genetic disruptions.
genomes
0.4
neutral
3XU9MCX6VOC8XAZD2V1AYSE50LC2RQ
biomed
These results demonstrate important epistatic interactions between genes from 129 and C57BL/6 genomes on the development of autoimmunity and illustrate the important effects of background genes in the analysis and interpretation of autoimmune phenotypes associated with targeted genetic disruptions.
genomes
0.46875
neutral
32TZXEA1OLKU7JFJ8F5JLGTQG1E14P
biomed
Dmrt7 is expressed only in the gonad, and, unlike the other Dmrt genes, appears to be present exclusively in mammals and not in nonmammalian vertebrates [23,36].
gonad
0.4
neutral
3JVP4ZJHDPSO81TGXL3N1CKZGQBI0I
biomed
Sry is not present in nonmammalian systems; however, antagonistic signaling between FGFs and WNTs may be the conserved mechanism that balances the gonad between testicular and ovarian fates in vertebrates.
gonad
0.5
neutral
3NFWQRSHVEE19E2BAFM5J7UN7F8FGR
biomed
In all species, the embryonic gonad is made up of a mixed population of germ cells and somatic cells.
gonad
0.5
neutral
3W0KKJIARR9XWLO7SS1E775URTAK8I
biomed
Many studies support the view that cells in the undifferentiated gonad are bipotential; the supporting cell precursor lineage can develop into follicle cells or Sertoli cells.
gonad
0.514706
neutral
3KA7IJSNW65H9AJU8WQWUQHUT88PBS
biomed
However, expression of Sertoli cell markers was not previously detected in these mutants during fetal stages [ 8, 29], leading to the conclusion that Wnt4 was not involved in primary sex determination in the gonad.
gonad
0.633333
difficult
31KPKEKW4ADELTFARBB6FPGML0X0BX
biomed
Moreover, no cataract formation was observed in the alphaB knockout lenses.
cataract
0.4
neutral
3D4BBDG7ZHWTCQ6DOYPKS7KB6XG3C1
biomed
Of the four AMPAR constituents, GluR-A to D (GluR1 to 4), which form tetrameric channels with different binary subunit combinations, GluR-B is contained in the majority of AMPARs.
constituents
0.4
neutral
3LN3BXKGC0V3K0O604W5NVGN2IYGWQ
biomed
Because synapsis occurs in TRIP13-deficient spermatocytes and is dependent on DSB formation (activity of SPO11 and MEI1), we suggest that TRIP13 functions after homology recognition and strand exchange, and that recombination events entering the CO repair pathway are either completed or nearly so (because OA treated resulted in bivalent chromosomes).
strand
0.402778
neutral
3ZG552ORAM40QHGUBF6KZP38LQR2VJ
biomed
Agouti protein has a short radius of action (Silvers and Russel 1955) and can be switched on and off during a single hair cycle (Bultman et al. 1992, 1994; Miller et al. 1993; Vrieling et al. 1994); thus, its regulated expression is thought to be responsible for the cream-colored or yellow ventral surface of mice carrying the black-and-tan (at) allele and for the yellow markings around the feet, ears, or head, i.e., tan points or head spots, of certain dog breeds.
Russel
0.402778
neutral
34R0BODSP1ZBN3DVY8J8XSIY55F5EH
biomed
The three members of the vertebrate TIF1 family (α, β, and γ) are large nuclear proteins that each contain an N-terminal RBCC or TRIM domain (Reymond et al. 2001) composed of a RING finger, two B-boxes, and a coiled-coil domain.
RING
0.402778
neutral
3K2CEDRACB1BKTGYG2PPDGTLUASTML
biomed
These data jointly indicate that battenin resides in a subset of vesicular compartments linking multiple membrane trafficking pathways, perhaps functioning in vesicular transport and/or fusion.
trafficking
0.404762
neutral
33N1S8XHHMLAAG38T8V9FC6ZHVP1Z7
biomed
In the platelet aggregation experiments the initial aggregation velocity was slightly, but significantly lower in platelets lacking annexin A7.
velocity
0.404762
neutral
3ZZAYRN1I6RZKW1ATI425KIQPB5OTV
biomed
While these apparent outcome disparities among experimental systems remain unclear, they could potentially result from variations in redundancies and compensatory mechanisms inherent to each experimental system.
variations
0.40625
neutral
3CMIQF80GNQW3A3ECIODJFLCK4C6QA
biomed
In addition, Ca2+ ions can drive spiculation of the membrane bilayer without involving the cytoskeleton [52].
ions
0.40625
neutral
3JHB4BPSFK9JLT5CAPRP3PCEA4MQ95
biomed
In neurons Ca2+ ions play major roles in various physiological and pathophysiological processes [20-25].
ions
0.425
neutral
3VGET1QSZ0ZKR7D571SBHI3U3FKW78
biomed
Annexin A7, the first annexin to be described, was isolated as the agent that mediated aggregation of chromaffin granules and fusion of membranes and phospholipids in the presence of Ca2+-ions [3].
ions
0.45
neutral
3CKVGCS3PG5D2FWC2XX15EQMPOE0ST
biomed
Biochemical studies also demonstrate that TIF1γ forms both homo-oligomers and hetero-oligomers with TIF1α but not with TIF1β (Peng et al. 2002).
Peng
0.407895
neutral
3XEDXEGFX3OFEWJ4XOOZESZC8U0K0V
biomed
Positional cloning identifies the mon gene as the zebrafish ortholog of mammalian transcriptional intermediary factor 1γ (TIF1γ), a member of the TIF1 family of transcriptional coactivators and corepressors (Le Douarin et al. 1995; Friedman et al. 1996; Kim et al. 1996; Venturini et al. 1999; Peng et al. 2002).
Peng
0.515625
neutral
3K3G488TR280K6307ZE8R97CO83Q5E
biomed
In contrast to our findings, Peng et al. [19] reported that M-opsin expression is mildly increased in the rd7 mutant retina.
Peng
0.535714
neutral
3YOAVL4CA0HR2F0RGQEBDAD2TFYZ4H
biomed
As illustrated in Figure 4 the 2M donor region exhibits strong sex effects on the rate of lipid storage, dependent on background.
lipid
0.410714
neutral
3WPCIUYH1A88GZAL39QMUQ6RLW0TD3
biomed
Furthermore, they contain sorcin and annexin A7 attached to the lipid rafts.
lipid
0.460526
neutral
3VJ4PFXFJ37PI5MYJ4PU9LKNJ7AAU9
biomed
Evidence suggests that the production of reactive oxygen species is a major effect of acute cadmium toxicity (68,69), and exposure of cultured cells or animals to cadmium is associated with depletion of reduced glutathione, lipid peroxidation and DNA damage (70–73).
lipid
0.483333
neutral
3LAZVA75NIR50X7XA3K64X90PX02ON
biomed
Furthermore, annexin A7 associates with lipid rafts [7].
lipid
0.515625
neutral
3JMNNNO3B14D56GZ1PBGLRMMSZZ2WZ
biomed
No significant differences between Slc26a4+/+ and Slc26a4-/- mice were found in perilymphatic (Fig. 2a) or plasma K+ concentrations (Slc26a4+/+, 4.9 ± 0.3 mM, n = 6; Slc26a4-/-, 5.1 ± 0.3 mM, n = 6).
K
0.410714
neutral
3ZG552ORAM40QHGUBF6KZP38LRP2VJ
biomed
Expression of key proteins involved in the generation of the endocochlear potential and the transport of K+ was studied using confocal immunocytochemistry and quantitative RT-PCR.
K
0.428571
neutral
3PKJ68EHDNWVECJIDBV8J5YGFN0JHX
biomed
However, several physiological studies have suggested that quinine can directly activate G proteins or cationic conductances, or can block K+ channels in taste receptor cells [34-36].
K
0.460526
neutral
3QREJ3J433XSBS8QMHAICCR0BSGKL9
biomed
K+ secretion and the generation of the endocochlear potential were measured using electrophysiological techniques.
K
0.5
neutral
3JHB4BPSFK9JLT5CAPRP3PCEA5KQ95
biomed
Since the constitutive Na+/K+ ATPase pump and the ATP-dependent conversion of glutamine to glutamate (both fundamental to maintain the electrical activity in the CNS) consumes the vast majority of the energy produced by the CNS [45–47], this likely underlies the suppression of the electrophysiological output responses of retinal neurons (Figure 8).
K
0.5
neutral
3G4VVJO6PZGI2DNOE93PTT0KG2CKP2
biomed
Thus it is tempting to relate the cardiac problems in the mouse to the apparent broader range of substrate utilization of mouse MCAD.
substrate
0.411765
neutral
3AQN9REUTFG4XL9FDFDL27Y60ZGDYC
biomed
The DNA substrate contains a pair of mutated GFP genes (GFP encodes the easily detected green fluorescent protein), one of which contains a restriction site for I-SceI, a yeast intron encoded endonuclease with an 18 base pair recognition site.
substrate
0.53125
neutral
3OB6JN3A9QPY4E36CDKUD1S36P0RMB
biomed
In this report, in order to clarify the mode of action of the Mcm helicase and obtain insight into how it may function at the replication forks in vivo, we have conducted detailed analyses of helicase action and DNA binding of mouse Mcm4/6/7 helicase using various forked and bubble substrate DNAs.
substrate
0.55
neutral
3SMIWMMK615MPIP1K9LMRAHEBQIUW9
biomed
The first step requires generating a floxed allele in ES cells that will serve as the substrate for subsequent exchanges (RMCE-ready ES cell, Figure 1).
substrate
0.555556
neutral
3TFJJUELSHP4R8AUKYBF9XFJ0JBC2U
biomed
Moynahan et al [24] have used such a GFP recombination substrate to demonstrate that cells with defective BRCA2 protein are deficient in their ability to repair the I-SceI-induced DSB through homologous recombination.
substrate
0.625
difficult
3YOAVL4CA0HR2F0RGQEBDAD2TFSZ4B
biomed
Several mutations and genes have been identified that affect the pattern of hair follicle development, but Tbx15 is the only gene of which we are aware that affects the pattern of hair pigmentation in different body regions.
follicle
0.411765
neutral
39RRBHZ0AU1QWSJ60MFYM0DF6ZPZVN
biomed
Expression of Sry in these bipotential cells leads to their differentiation as testis-specific Sertoli cells rather than as follicle cells, the parallel cell type of the ovary [ 2].
follicle
0.446429
neutral
3H1C3QRA01JWMOMADI6GBYO8XM7CE2
biomed
Many studies support the view that cells in the undifferentiated gonad are bipotential; the supporting cell precursor lineage can develop into follicle cells or Sertoli cells.
follicle
0.515625
neutral
3P6ENY9P79WZJ1ZCVTA874JG27JHIP
biomed
However, earlier findings of normal proprioceptive afferent trajectories in chicken embryos despite injection of function-blocking NT-3 antibody into the spinal cord (Oakley et al. 1995) led them to discount this possibility.
trajectories
0.411765
neutral
3A9LA2FRWSEW9WO7UFA9AE6VQKMXH4
biomed
Interestingly, heterozygous crosses involving the Capn4 transgenic line generated by Zimmerman and colleagues yielded progeny with a similar, if less extreme, skewing in favor of the heterozygous genotype.
skewing
0.411765
neutral
3909MD9T2ZH3P06K4KWHJ49BUZZFEG
biomed
Studies of X-chromosome inactivation in carrier females have demonstrated preferential inactivation of the chromosome bearing the abnormal allele in a variety of tissues [2], and this skewing of X-inactivation is thought to explain the mild phenotype observed in carriers.
skewing
0.483333
neutral