title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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The Milwaukee Inventory for the Dimensions of Adult Skin Picking (MIDAS): initial development and psychometric properties. | This article describes the development and initial psychometric properties of the Milwaukee Inventory for the Dimensions of Adult Skin picking (MIDAS), a measure designed to assess "automatic" and "focused" skin picking. Data were collected from 92 participants who completed an anonymous internet-based survey. Results ... | 18,725,154 |
Cost-minimization analysis of jumbo reusable forceps versus disposable forceps in a high-volume ambulatory endoscopy center. | Endoscopists worldwide are faced with the challenge of choosing the most cost-effective and durable equipment. There are limited data comparing the 2 major options for endoscopic forceps: disposable and reusable. Disposable forceps are marketed as the cost-effective alternative to reusable forceps. This study was desig... | 18,725,156 |
Nephrin and podocin loss is prevented by mycophenolate mofetil in early experimental diabetic nephropathy. | Several works in the setting of early experimental diabetic nephropathy using anti-inflammatory drugs, such as mycophenolate mofetil (MMF), have shown that prevention of the development or amelioration of renal injury including proteinuria. The exact mechanisms by which anti-inflammatory drugs lower the albuminuria hav... | 18,725,182 |
A Q312X mutation in the hemojuvelin gene is associated with cardiomyopathy due to juvenile haemochromatosis. | Juvenile haemochromatosis (JH) is an autosomal recessive iron disorder characterized by the early onset of secondary cardiomyopathy. The candidate modifier genes are hemojuvelin (HJV) and hepcidin antimicrobial peptide (HAMP). In the Japanese population, the prevalence of JH is quite low. The influence of HJV mutation ... | 18,725,184 |
Immunotherapy of hepatocellular carcinoma with a vaccine based on xenogeneic homologous alpha fetoprotein in mice. | alpha-Fetoprotein (AFP) is a diagnostic marker for the presence of hepatocellular carcinoma, and a potential target for immunotherapy. Unfortunately, the immunity to AFP is presumably difficult to elicit because of immune tolerance acquired during the development of immune system. In the present study, we used AFP as a... | 18,725,206 |
In silico identification of anthropogenic chemicals as ligands of zebrafish sex hormone binding globulin. | Anthropogenic compounds with the capacity to interact with the steroid-binding site of sex hormone binding globulin (SHBG) pose health risks to humans and other vertebrates including fish. Building on studies of human SHBG, we have applied in silico drug discovery methods to identify potential binders for SHBG in zebra... | 18,725,242 |
Dual-probe real-time PCR assay for detection of variola or other orthopoxviruses with dried reagents. | A real-time, multiplexed polymerase chain reaction (PCR) assay based on dried PCR reagents was developed. Only variola virus could be specifically detected by a FAM (6-carboxyfluorescein)-labeled probe while camelpox, cowpox, monkeypox and vaccinia viruses could be detected by a TET (6-carboxytetramethylrhodamine)-labe... | 18,725,245 |
Are oscillatory brain responses generally reduced in schizophrenia during long sustained attentional processing? | Deficits in sustained attention and vigilance were assessed for oscillatory delta, theta, alpha, and gamma EEG activity during an auditory continuous performance task in patients with schizophrenia and healthy controls by quantifying peak-to-peak amplitudes of averaged and single-trial data. Averaged data indicated sig... | 18,725,254 |
Targeting subcapsular antigens for prevention of Klebsiella pneumoniae infections. | Vaccination strategies against Klebsiella pneumoniae have largely focussed on the polysaccharide capsule. However, the large number and high prevalence of individual capsular serotypes limits the widespread applicability of capsule-based vaccines. This study establishes that immunization with purified LPS can protect m... | 18,725,260 |
Aflatoxin B1-induced TP53 mutational pattern in normal human cells using the FASAY (Functional Analysis of Separated Alleles in Yeast). | Mutations in the TP53 gene are the most common alterations in human tumours. In hepatocellular carcinoma (HCC) related to exposure to aflatoxin B1, a specific G>T transversion in codon 249 is classically described as a hot spot. However, AFB1 is suspected to be a potent carcinogen in tissues other than the liver. By... | 18,725,321 |
Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa. | Reactive oxygen species (ROS) induction by exposure to heavy metals (Cd, Cu or Zn) in diverse free-living ciliated protozoa (Tetrahymena sp. and three strains of Colpoda steinii, isolated from freshwater and soils with different level of metal pollution) has been evaluated. Using specific fluorophores, such as 2',7'-di... | 18,725,323 |
[A monoclonal immunoglobulin M not visible on the profile of serum capillary electrophoresis]. | We report a case of an invisible monoclonal IgM on the profile of the serum electrophoresis carried out by the Paragon CZE 2000 system. This case is about a 84 years old man followed for a Waldenström disease. The level of the monoclonal IgM given at the time of the preceding visit one year before was 10 g/L. The elect... | 18,725,351 |
[PPAR alpha-L162V, frequency and relationship with type 2 diabetes among black Senegalese people]. | PPARs are supposed to be involved in pathogenesis of diabetes and its complications. According to some authors, L162V PPARalpha gene polymorphism would be associated to dyslipidemia susceptibility during diabetes, whereas for some authors, it rather would confer resistance to these metabolic abnormalities. The aim of t... | 18,725,353 |
The impact of statin use on atrial fibrillation. | The aim of the present systematic review is to present an overview of the evidence linking atrial fibrillation (AF), inflammation and oxidative stress, with emphasis on the potential of statins to decrease the incidence of different types of AF, including new-onset AF, after electrical cardioversion (EC) and after card... | 18,725,372 |
Obtaining utility estimates of the health value of commonly prescribed treatments for asthma and depression. | Comparing the costs and health value associated with alternative quality improvement efforts is useful. This study employs expert panel methodology to elicit numerical estimates based on a 0 to 1 utility scale of the health benefit of usual treatment patterns for 2 medical conditions. The approach includes development ... | 18,725,407 |
Functional adhesiveness of the CX3CL1 chemokine requires its aggregation. Role of the transmembrane domain. | In its native form, the chemokine CX3CL1 is a firmly adhesive molecule promoting leukocyte adhesion and migration and hence involved, along with its unique receptor CX3CR1, in various inflammatory processes. Here we investigated the role of molecular aggregation in the CX3CL1 adhesiveness. Assays of bioluminescence res... | 18,725,411 |
The diversity of O-linked glycans expressed during Drosophila melanogaster development reflects stage- and tissue-specific requirements for cell signaling. | Appropriate glycoprotein O-glycosylation is essential for normal development and tissue function in multicellular organisms. To comprehensively assess the developmental and functional impact of altered O-glycosylation, we have extensively analyzed the non-glycosaminoglycan, O-linked glycans expressed in Drosophila embr... | 18,725,413 |
Protein-tyrosine phosphatase alpha regulates stem cell factor-dependent c-Kit activation and migration of mast cells. | The role of protein-tyrosine phosphatase alpha (PTPalpha) in mast cell function was investigated in tissues and cells from PTPalpha-deficient mice. Bone marrow-derived mast cells (BMMCs) lacking PTPalpha exhibit defective stem cell factor (SCF)-dependent polarization and migration. Investigation of the molecular basis ... | 18,725,415 |
Molecular basis of the Bohr effect in arthropod hemocyanin. | Flash photolysis and K-edge x-ray absorption spectroscopy (XAS) were used to investigate the functional and structural effects of pH on the oxygen affinity of three homologous arthropod hemocyanins (Hcs). Flash photolysis measurements showed that the well-characterized pH dependence of oxygen affinity (Bohr effect) is ... | 18,725,416 |
Delta protein kinase C interacts with the d subunit of the F1F0 ATPase in neonatal cardiac myocytes exposed to hypoxia or phorbol ester. Implications for F1F0 ATPase regulation. | Mitochondrial protein kinase C isozymes have been reported to mediate both cardiac ischemic preconditioning and ischemia/reperfusion injury. In addition, cardiac preconditioning improves the recovery of ATP levels after ischemia/reperfusion injury. We have, therefore, evaluated protein kinase C modulation of the F(1)F(... | 18,725,417 |
ZapA, a virulence factor in a rat model of Proteus mirabilis-induced acute and chronic prostatitis. | Our knowledge of pathogenesis has benefited from a better understanding of the roles of specific virulence factors in disease. To determine the role of the virulence factor ZapA, a 54-kDa metalloproteinase of Proteus mirabilis, in prostatitis, rats were infected with either wild-type (WT) P. mirabilis or its isogenic Z... | 18,725,420 |
The roles of physiological and subjective stress in the effectiveness of a placebo on experimentally induced pain. | To examine whether reduction of negative emotions and associated autonomic activity could explain placebo analgesia, and to test the effect of experimenter gender on the placebo analgesic response. Sixty-three (n = 32 females) students participated in a within-subjects design where subjects were tested on two separate ... | 18,725,424 |
Persistence of posttraumatic stress symptoms 12 and 36 months after acute coronary syndrome. | To assess the prevalence and predictors of posttraumatic stress symptoms in patients at 12 and 36 months post hospital admission for an acute coronary syndrome (ACS). There is increasing recognition that posttraumatic stress may develop in the aftermath of an acute cardiac event. However, there has been little research... | 18,725,431 |
Dominance of blaCTX-M within an Australian extended-spectrum beta-lactamase gene pool. | bla(CTX-M) genes, particularly bla(CTX-M-15), are the dominant extended-spectrum beta-lactamase (ESBL) genes among clinical isolates of Escherichia coli and Klebsiella pneumoniae in Sydney, Australia, where we also found one example of bla(CTX-M-62), encoding a novel enzyme conferring ceftazidime resistance. ESBL genes... | 18,725,449 |
Arterial calcification in race horses. | Calcification of large arteries has been sporadically reported in horses. The pathogenesis is still unknown, but recent studies in humans suggest that this is a regulated biomineralizing process. This study surveyed the prevalence, distribution, and severity of vascular calcification in Thoroughbred and Standardbred ra... | 18,725,464 |
Systemic reactive angioendotheliomatosis-like syndrome in a steer presumed to be persistently infected with bovine viral diarrhea virus. | Unusual proliferative intravascular lesions were seen in multiple organs of a 2-year-old Corriente steer presumed to be persistently infected with bovine viral diarrhea virus (BVDV), based on widespread immunohistochemical detection of BVDV antigen. Proliferations of spindle cells, which were immunohistochemically posi... | 18,725,468 |
Ganglioneuroma of the brachial plexus in two cockatiels (Nymphicus hollandicus). | Ganglioneuroma involving the brachial plexus, paraspinal ganglia, and cervical-thoracic spinal cord was diagnosed in 2 adult cockatiels (Nymphicus hollandicus). Both birds had a chronic 1-year history of ataxia and perching difficulty. At necropsy, each bird had a unilateral, firm, gelatinous white to tan multilobular ... | 18,725,475 |
Dynamic mechanism for initiation of ventricular fibrillation in vivo. | Dynamically induced heterogeneities of repolarization may lead to wave-front destabilizations and initiation of ventricular fibrillation (VF). In a computer modeling study, we demonstrated that specific sequences of premature stimuli maximized dynamically induced spatial dispersion of refractoriness and predisposed the... | 18,725,487 |
Identification of hydroxywarfarin binding site in human UDP glucuronosyltransferase 1a10: phenylalanine90 is crucial for the glucuronidation of 6- and 7-hydroxywarfarin but not 8-hydroxywarfarin. | Recent studies show that the extrahepatic human UDP-glucuronosyltransferase (UGT)1A10 is capable of phase II glucuronidation of several major cytochrome P450 metabolites of warfarin (i.e., 6-, 7-, and 8-hydroxywarfarin). This study expands on this finding by testing the hypothesis that the UGT1A10 F(90)-M(91)-V(92)-F(9... | 18,725,508 |
A crucial role for hnRNP K in axon development in Xenopus laevis. | We report that hnRNP K, an RNA-binding protein implicated in multiple aspects of post-transcriptional gene control, is essential for axon outgrowth in Xenopus. Its intracellular localization was found to be consistent with one of its known roles as an mRNA shuttling protein. In early embryos, it was primarily nuclear, ... | 18,725,517 |
Toll-like receptor and IL-12 signaling control susceptibility to contact hypersensitivity. | Allergic contact hypersensitivity (CHS) is a T cell-mediated inflammatory skin disease. Interleukin (IL)-12 is considered to be important in the generation of the allergen-specific T cell response. Loss of IL-12 function in IL-12Rbeta2-deficient mice, however, did not ameliorate the allergic immune response, suggesting... | 18,725,520 |
Phosphorylation of the Arp2/3 complex is necessary to nucleate actin filaments. | The actin-related protein 2/3 (Arp2/3) complex is the primary nucleator of new actin filaments in most crawling cells. Nucleation-promoting factors (NPFs) of the Wiskott-Aldrich syndrome protein (WASP)/Scar family are the currently recognized activators of the Arp2/3 complex. We now report that the Arp2/3 complex must ... | 18,725,535 |
Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum. | Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and occurs near the endoplasmic reticulum (ER), but the exact mechanisms are unknown. We show that double FYVE domain-containing ... | 18,725,538 |
SopB promotes phosphatidylinositol 3-phosphate formation on Salmonella vacuoles by recruiting Rab5 and Vps34. | Salmonella colonizes a vacuolar niche in host cells during infection. Maturation of the Salmonella-containing vacuole (SCV) involves the formation of phosphatidylinositol 3-phosphate (PI(3)P) on its outer leaflet. SopB, a bacterial virulence factor with phosphoinositide phosphatase activity, was proposed to generate PI... | 18,725,540 |
uPAR promotes formation of the p130Cas-Crk complex to activate Rac through DOCK180. | The urokinase-type plasminogen activator receptor (uPAR) drives tumor cell membrane protrusion and motility through activation of Rac; however, the pathway leading from uPAR to Rac activation has not been described. In this study we identify DOCK180 as the guanine nucleotide exchange factor acting downstream of uPAR. W... | 18,725,541 |
Condom 'turn offs' among adults: an exploratory study. | An exploratory study compared the prevalence of multiple types of condom-associated 'turn offs' in men and women. Nearly 2000 people completed a web-based questionnaire. Data were analysed from 464 men and women who reported that condoms had turned them off the last time they were used. Gender differences were not obse... | 18,725,548 |
Cognitive assessment and quantitative magnetic resonance metrics can help to identify benign multiple sclerosis. | The definition of benign multiple sclerosis (B-MS) is still controversial. This mainly takes into account the subject's motor ability, with little or no relevance to other important features such as cognition. Moreover, no paraclinical markers are currently available to reliably identify patients who will remain benign... | 18,725,589 |
Natural history of SMA IIIb: muscle strength decreases in a predictable sequence and magnitude. | To assess the natural progression of muscle weakness in spinal muscular atrophy (SMA) IIIb. Ten patients with SMA IIIb were followed for at least 10 years. Age at disease onset varied between 9 and 18 years. Patients were initially seen 2 to 10 years after disease onset. They were evaluated at approximately 2, 5, 10, 1... | 18,725,590 |
Second consensus statement on the diagnosis of multiple system atrophy. | A consensus conference on multiple system atrophy (MSA) in 1998 established criteria for diagnosis that have been accepted widely. Since then, clinical, laboratory, neuropathologic, and imaging studies have advanced the field, requiring a fresh evaluation of diagnostic criteria. We held a second consensus conference in... | 18,725,592 |
Inhibition of the fungal fatty acid synthase type I multienzyme complex. | Fatty acids are among the major building blocks of living cells, making lipid biosynthesis a potent target for compounds with antibiotic or antineoplastic properties. We present the crystal structure of the 2.6-MDa Saccharomyces cerevisiae fatty acid synthase (FAS) multienzyme in complex with the antibiotic cerulenin, ... | 18,725,634 |
Lignin degradation in wood-feeding insects. | The aromatic polymer lignin protects plants from most forms of microbial attack. Despite the fact that a significant fraction of all lignocellulose degraded passes through arthropod guts, the fate of lignin in these systems is not known. Using tetramethylammonium hydroxide thermochemolysis, we show lignin degradation b... | 18,725,643 |
Twin hook fixation for proximal femoral fractures. | To report results of twin hook fixation for proximal femoral fractures in comparison to those fixed with the conventional lag screw. Between August 2005 and July 2006, 2 men and 15 women aged 74 to 94 (mean, 85) years with proximal femoral fractures underwent open reduction and internal fixation using the twin hook sys... | 18,725,664 |
Osteosynthesis for a T-shaped fracture of the femoral neck and trochanter: a case report. | Ipsilateral fractures of the femoral neck and trochanter are uncommon. We report a 30-year-old man with a T-shaped fracture separating the trochanter and neck from the head. He underwent osteosynthesis using a dynamic compression plate, screws, and pins. Bone union occurred 4 months postoperatively. At the one-year fol... | 18,725,685 |
Surgical wound mucormycosis necessitating hand amputation: a case report. | We report an unusual case of cutaneous mucormycosis in a 17-year-old man who had no risk factors for fungal infection. The aggressive nature of cutaneous mucormycosis is illustrated. A high index of suspicion is crucial for identifying and preventing progression of this disease, which can lead to limb amputation, even ... | 18,725,688 |
Carrying loads and postural sway in standing: the effect of load placement and magnitude. | This study investigated the effect that load magnitude, load location, and the dimensions that the base of support have on postural sway in standing while wearing a backpack, single strapped bag, briefcase, or purse. Subjects were instructed to carry a load of 10% or 20% of their body weight with either their feet spac... | 18,725,699 |
Which work factors determine job satisfaction? | Job satisfaction is associated with mental health. Employees could be counselled on how they feel about their work. If specific aspects of their job are causing particular dissatisfaction, they could be assisted to appropriately change these aspects. There is no 'gold standard' indicating the aspects that should be tak... | 18,725,706 |
Marine macroalga Sargassum horneri as biosorbent for heavy metal removal: roles of calcium in ion exchange mechanism. | Brown seaweed Sargassum horneri, a troublesome biomass scattered along the seashore, was utilized as a biosorbent for Pb(II) removal from aqueous solutions. The Pb(II) adsorption by brown seaweed was enhanced by pretreatment with CaCl(2), and the Langmuir adsorption isotherm equation showed a maximum capacity of a Q(ma... | 18,725,741 |
Arginine, nitric oxide, carbon monoxide, and endothelial function in severe malaria. | Parasiticidal therapy of severe falciparum malaria improves outcome, but up to 30% of these patients die despite best therapy. Nitric oxide is protective against severe disease, and both nitric oxide and arginine (the substrate for nitric oxide synthase) are low in clinical malaria. Parasitized red blood cell interacti... | 18,725,795 |
Quality of life in patients with idiopathic sudden hearing loss: comparison of different therapies using the Medical Outcome Short Form (36) Health Survey questionnaire. | Cause and underlying pathogenic mechanisms of idiopathic sudden hearing loss (ISHL) are not fully understood, resulting in the widespread use of different polypragmatic treatment approaches, which have not been finally validated in randomized controlled trials. Quality of life (QoL) can provide helpful additional infor... | 18,725,858 |
Urinary sucrose and fructose as biomarkers of sugar consumption: comparison of normal weight and obese volunteers. | Using urinary sugars as a biomarker of consumption, we have previously shown that obese people consume significantly more sugars than individuals of normal weight. However, there is concern that recovery of this biomarker may differ between normal weight and obese individuals. A total of 19 subjects, divided into two g... | 18,725,895 |
Epigenetic control of myelin repair. | Although the CNS has a robust innate ability to repair demyelinated axons, this capacity appears to dissipate with age. A study in this issue suggests that epigenetic processes participate in myelin repair and that the epigenetic response is less dynamic in older individuals. | 18,725,899 |
Neuropeptide receptor transcriptome reveals unidentified neuroendocrine pathways. | Neuropeptides are an important class of molecules involved in diverse aspects of metazoan development and homeostasis. Insects are ideal model systems to investigate neuropeptide functions, and the major focus of insect neuropeptide research in the last decade has been on the identification of their receptors. Despite ... | 18,725,956 |
The actin-binding protein capulet genetically interacts with the microtubule motor kinesin to maintain neuronal dendrite homeostasis. | Neurons require precise cytoskeletal regulation within neurites, containing microtubule tracks for cargo transport in axons and dendrites or within synapses containing organized actin. Due to the unique architecture and specialized function of neurons, neurons are particularly susceptible to perturbation of the cytoske... | 18,725,959 |
Psychosocial Moderators the Effects of Transitioning Into Filial Caregiving on Mental and Physical Health. | A life-course theoretical perspective guided this study to examine how effects on mental and physical health (depressive symptoms, hostility, global happiness, self-esteem, personal mastery, psychological wellness, self-rated physical health) of transitioning into filial caregiving for a sole surviving parent are moder... | 18,725,964 |
Cre-dependent expression of multiple transgenes in isolated neurons of the adult forebrain. | Transgenic mice with mosaic, Golgi-staining-like expression of enhanced green fluorescent protein (EGFP) have been very useful in studying the dynamics of neuronal structure and function. In order to further investigate the molecular events regulating structural plasticity, it would be useful to express multiple protei... | 18,725,976 |
PPARalpha Ligands as Antitumorigenic and Antiangiogenic Agents. | Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear receptor family of ligand-activated transcription factors. This subfamily is composed of three members-PPARalpha, PPARdelta, and PPARgamma-that differ in their cell and tissue distribution as well as in their target genes. PPARalpha is abundantly... | 18,725,983 |
Misfolded proteins activate factor XII in humans, leading to kallikrein formation without initiating coagulation. | When blood is exposed to negatively charged surface materials such as glass, an enzymatic cascade known as the contact system becomes activated. This cascade is initiated by autoactivation of Factor XII and leads to both coagulation (via Factor XI) and an inflammatory response (via the kallikrein-kinin system). However... | 18,725,990 |
Platelet glycoprotein Ibalpha forms catch bonds with human WT vWF but not with type 2B von Willebrand disease vWF. | Arterial blood flow enhances glycoprotein Ibalpha (GPIbalpha) binding to vWF, which initiates platelet adhesion to injured vessels. Mutations in the vWF A1 domain that cause type 2B von Willebrand disease (vWD) reduce the flow requirement for adhesion. Here we show that increasing force on GPIbalpha/vWF bonds first pro... | 18,725,999 |
Transcription monitoring using fused RNA with a dye-binding light-up aptamer as a tag: a blue fluorescent RNA. | The "light-up" RNA aptamer-Hoechst pair can be used as a fluorescent tag to monitor transcription processes. | 18,726,014 |
Giant molecular spoked wheels in giant voids: two-dimensional molecular self-assembly goes big. | We present here the formation of giant pores in surface-confined molecular networks of a triangular-shaped dehydrobenzo-[12]annulene derivative: the diameter of the pores reaches over 7 nm and the giant pores are used as templates to accommodate a giant molecular spoked wheel, which allows us to observe rotational and ... | 18,726,027 |
Manipulating the cavity of a porous material changes the photoreactivity of included guests. | Changing an ether to a ketone within the framework of a bis-urea macrocycle has little effect on the supramolecular assembly of this building block into porous crystals but introduces a triplet sensitizer into the framework that dramatically alters the photochemical reactions of included guests. | 18,726,031 |
A photosensitive {Ru-NO}6 nitrosyl bearing dansyl chromophore: novel NO donor with a fluorometric on/off switch. | The synthesis, fluorescence properties and NO photolability of [(Me(2)bpb)Ru(NO)(Ds-im)]BF(4), a {Ru-NO}(6) nitrosyl-fluorophore conjugate, have been investigated and its potential as a trackable NO donor has been evaluated. | 18,726,039 |
[Cognitive impairment in normal pressure hydrocephalus (NPH). A proposal for clinical evaluation protocol]. | Normal pressure hydrocephalus (NPH) is characterized by gait disturbance, urinary incontinence and dementia, and is associated with variable ventricular enlargement. The most accepted treatment of NPH is the placement of a cerebrospinal fluid shunt. Owing to the characteristics of the patients and the invasive nature o... | 18,726,041 |
[Spontaneous resolution of an asymptomatic intracranial arachnoid cyst]. | Arachnoid cysts are commonly considered to be benign, congenial, extraparenchymatous anomalies. Small cyst are common incidental findings in children and adults. The aetiology and natural history of arachnoid cysts are not fully understood. In most cases, the presence of the cysts is detected on CT-scans or MRI perform... | 18,726,048 |
A cystic amelanotic melanoma metastasis to the brain: case report. | As far as we know, cyst formation in intracranial melanoma is rare, and only 15 cases of intracranial amelanotic melanoma have been reported until now. A yellowish mass was observed in the frontal lobe. The content of the cyst consisted of old hematoma, xanthochromic fluid and necrotic tissue, was evacuated and the cys... | 18,726,049 |
Biodiversity offsets: two New Zealand case studies and an assessment framework. | Biodiversity offsets are increasingly being used for securing biodiversity conservation outcomes as part of sustainable economic development to compensate for the residual unavoidable impacts of projects. Two recent New Zealand examples of biodiversity offsets are reviewed-while both are positive for biodiversity conse... | 18,726,050 |
Adaptation as a political process: adjusting to drought and conflict in Kenya's drylands. | In this article, we argue that people's adjustments to multiple shocks and changes, such as conflict and drought, are intrinsically political processes that have uneven outcomes. Strengthening local adaptive capacity is a critical component of adapting to climate change. Based on fieldwork in two areas in Kenya, we inv... | 18,726,051 |
Transport at the nanoscale: temperature dependence of ion conductance. | Temperature dependent ion conductance in nanopores is measured in a wide range of electrolyte concentrations and compared with molecular modeling. Single outer membrane protein F (OmpF) channels from E. coli are reconstituted into planar lipid bilayers. In qualitative agreement with the experimental data, applied-field... | 18,726,094 |
Fetal therapy in twin reserve arterial perfusion sequence pregnancies with alcohol ablation or bipolar cord coagulation. | We aimed to evaluate perinatal outcome of seven pregnancies with twin reserve arterial perfusion sequence. Study group included seven cases of acardiac twins. Out of seven acardiac twins, two cases were followed without interventions. We performed four alcohol ablation and one bipolar coagulation. For alcohol ablation,... | 18,726,110 |
Physical activity, exercise, depression and anxiety disorders. | There is a general belief that physical activity and exercise have positive effects on mood and anxiety and a great number of studies describe an association of physical activity and general well-being, mood and anxiety. In line, intervention studies describe an anxiolytic and antidepressive activity of exercise in hea... | 18,726,137 |
Prediction of protein structural classes by Chou's pseudo amino acid composition: approached using continuous wavelet transform and principal component analysis. | A prior knowledge of protein structural classes can provide useful information about its overall structure, so it is very important for quick and accurate determination of protein structural class with computation method in protein science. One of the key for computation method is accurate protein sample representation... | 18,726,140 |
Phosphoinositides as regulators of membrane trafficking in health and disease. | Membrane trafficking is crucial in the homeostasis of the highly compartmentalized eukaryotic cells. This compartmentalization occurs both at the organelle level, with distinct organelles maintaining their identities while also intensely interchanging components, and at a sub-organelle level, with adjacent subdomains o... | 18,726,176 |
Membrane traffic in the secretory pathway. | During the last 20 years remarkable achievements have been made in the understanding of the molecular basis of membrane traffic in the secretory pathway. A combination of morphological, biochemical and genetical approaches revealed the identity of various compartments and transport intermediates, and provided basic fun... | 18,726,181 |
Possibility of biological micromachining used for metal removal. | Besides the physical and chemical machining methods, a biological machining method has been presented. The experimental results show that machining of pure iron, pure copper and constantan by a special bacterium,Thiobacillus ferrooxidans, was possible. A micro gear and grooves on pure copper piece were bio-machined. Th... | 18,726,199 |
Monoclonal antibody, a novel probe for protein folding. | Two monoclonal antibodies (McAb2C9, McAb1E5) against Staphylococcal nuclease R (SNase R) and its N-terminal peptide fragments were prepared, purified and characterized. Further studies show that the intact enzyme SNase R and its seven N-terminal peptide fragments differ in their interaction with McAb2C9. SNase R, SNR12... | 18,726,201 |
Membrane molecules in induction of apoptosis of thymocytes by mouse thymic dendritic cells which express Fas ligands. | Apoptosis of thymocytes is involved in the negative selection of thymus, but it remains unclear how the cell death of thymocytes is regulated by the thymic stromal cells. By using immunohistochemistry, TdT-mediated dUTP nick end labeling (TUNEL) and flow cytometry methods, it was found that Fas ligand was expressed in ... | 18,726,205 |
Isolation and partial molecular characterization of heat-stable growth factor from human placenta. | A heat-stable growth factor has been purified to homogeneity from human placenta. Purification procedure involved extraction with acidic medium, precipitation using ethanol, DE-52 ion-exchange chromatography, and reversed-phase HPLC. The purified preparation has been named human placental growth factor-1 (HPGF-1), whic... | 18,726,219 |
Raman spectroscopic study of DNA after photosensitive damage caused by hypocrellins A and B. | Laser Raman spectroscopy provided the direct evidence for microcosmic photodamage in space structure of DNA molecule. When photosensitive damage was made to space structure of calf thymus DNA by hypocrellins A and B. the Raman characteristic frequencies and the intensities of the bands assigned to various groups of the... | 18,726,251 |
Expression and regulation of hFIX minigene and cDNA driven by beta-casein gene in mouse mammary gland. | Mammary gland specific expression vectors for human clotting factor IX (hFIX) and LacZ reporter gene driven by bovine beta-casein gene were constructed. Vectors were packaged by stearylamine (SA) liposome and were transferred to lactating mice via tail vein. Both hFIX and Lac2 gene could be expressed in the mammary gla... | 18,726,258 |
Spatiotemporal organization of simple-cell receptive fields in area 18 of cat's cortex. | Spatiotemporal structures of receptive-fields (RF) have been studied for simple cells in area 18 of eat by measuring the temporal transfer function (TTF) over different locations (subregions) within the RF. The temporal characteristics of different subregions differed from each other in the absolute phase shift (APS) t... | 18,726,264 |
Isolation, properties and spatial site analysis of gamma subunits of B-phycoerythrin and R-phycoerythrin. | Polysiphonia urceolata R-phycoerythrin and Porphyridium cruentum B-phycoerythrin were degraded with proteinaseK, and then the nearly native gamma subunits were isolated from the reaction mixture. The process of degradation of phycoerythrin with proteinaseK showed that the gamma subunit is located in the central cavity ... | 18,726,265 |
A modified hepatitis B surface antigen carrying both preS1 and preS2 epitopes. | The DNA fragments coding for preS2(120-146) and preS1(21-47) amplified by PCR were fused to both 5' and 3' ends of S gene at the position of amino acid 223. The fusion gene was placed downstream of the promoter P7.5 of the universal vaccinia viral vector pGJP-5 and the recombinant vaccinia virus vS2SSI was then selecte... | 18,726,271 |
Fine structure and assembly in vitro of nuclear lamina in plant cells. | The fine structure of the nuclear lamina (NL) in sperm cells of Ginkgo biloba was visualised using high resolution low-voltage scanning electron microscopy (LVSEM). It was shown that the nuclear lamina was composed of 10 nm filaments which formed a fine network. Lamins were purified from cultured carrot suspension cell... | 18,726,273 |
Immunomodulated signaling in macrophages: Studies on activation of Raf-1, MAPK, cPLA(2) and secretion of IL-12. | Little is known about the mechanism and signal transduction by LPS-mediated immunomodulation of murine peritoneal macrophages. It is found that the signal molecules of the down-stream of Ras, Raf-1, MAPK p44, and MAPK p42 are phosphorylated, and cPLA(2) is activated with a significant increase of the release of [ H(3) ... | 18,726,282 |
Effect of lysophosphatidylcholine on behavior and structure of phosphatidylcholine liposomes. | Differential scanning calorimetry (DSC), fluorescence polarization and X-ray diffraction were performed to investigate the kinetics of the micellar to the lamellar phase transition of dipalmitoylphosphatidylcholine/1-palmitoylphosphatidylcholine (16:0 LPC/DPPC) liposomes at gel phase. With a 16:0 LPC concentration up t... | 18,726,286 |
Molecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core protein. | To further elucidate the molecular mechanisms underlying the suppression of hepatitis B virus (HBV) expression by the hepatitis C virus (HCV) core protein, five molecular clones of HCV cDNA sequence containing the 5' noncoding (5'NC) and the core regions have been isolated from Chinese HBV- and HCV-coinfected patients.... | 18,726,290 |
Exon structure requirements for yeast tRNA ligase. | Different nucleotides were introduced into nucleotides 32, 37 and 38 of yeast tRNA(Phe) precursors via oligonucleotide directed mutations. Pre-tRNAs were prepared using T7-transcription in vitro and spliced with the purified yeast tRNA endonuclease and tRNA ligase. It is demonstrated that tRNA ligase activities will be... | 18,726,292 |
Sequence analysis of lacZ(-) mutations induced by ion beam irradiation in double-stranded M13mp18DNA. | While M13mp18 double-stranded DNA was irradiated with ion beam, and transfected intoE. coli JM103, a decrease of transfecting activity was discovered. The lacZ(-) mutation frequency at 20% survival could reach (3.6-16.8) x 10(4), about 2, 3-10 times that of unirradiated M13DNA. Altogether, 27 IacZ(-)mutants were select... | 18,726,305 |
Influences of cerebellar interpositus nucleus and fastigial nucleus on neuronal activity of lateral hypothalamic area. | Stimulation of cerebellar interpositus nucleus and fastigial nucleus could influence the neuronal activity of lateral hypothalamic area in the cat, and some of the neurons which respond to the cerebellar stimulations are glucose-sensitive neurons. These results suggest that the cerebellum is involved not only in motor ... | 18,726,314 |
Combined therapy of p53-wt and drug in an orthotopic multidrug-resistant human lung cancer model. | Balb/c nu/nu mice were inoculated intratracheally with multidrug-resistant human lung cancer cells GLK containing p53 mutation at codon 245 and treated with intratracheal instillation of p53-wt retroviral vector (pDOR53W) to increase cell chemosensitivity, and then with intraperitoneal injection of doxorubicin. 30 d af... | 18,726,321 |
Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes. | Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. T... | 18,726,342 |
Comparative study on direction selectivity and functional organization of the primary visual cortical cells in monkeys and cats. | Although the directionally selective cells in many visual cortical areas are organized in columnar manner, the functional organization of direction selectivity of area Vl in the monkey still remains unclear. We quantitatively studied the proportion of directionally selective cells, direction selectivity and the functio... | 18,726,351 |
Mediation by calcium/calmodulin-dependent protein kinase II of suppression of GABA(A) receptors by NMDA. | Using nystatin-perforated whole-cell recording configuration, the modulatory effect of N-methyl-D-aspartate (NMDA) on gamma-aminobutyric acid (GABA)-activated whole-cell currents was investigated in neurons freshly dissociated from the rat sacral dorsal commissural nucleus (SDCN). The results showed that: (i) NMDA supp... | 18,726,361 |
Differential recognition of MHC class I molecules of xeno-/allo-endothelial cells by human NK cells. | Using human umbilical vein endothelial cells (HUVEC) and porcine aortic endothelial cells (PAEC) as target cells, human peripheral blood NK cells (PBNK) and NK92 cells as effector cells, the differential cytotoxicities of NK cells to allo- and xeno-endothelial cells were studied. The influence of MHC class I molecules ... | 18,726,370 |
Expansion of CD34(+) cells from human umbilical cord blood by FL and/or TPO gene transfected human marrow stromal cell lines. | To elucidate the effect of gene transfected marrow stromal cell on expansion of human cord blood CD34(+) cells, a culture system was established in which FL and TPO genes were transfected into human stromal cell line HFCL. To establish gene transfected stromal cells co-culture system, cord blood CD34(+) cells were puri... | 18,726,391 |
Comparative study of symmetric and asymmetric somatic hybridization between common wheat andHaynaldia villosa. | Symmetric and asymmetric protoplast fusion between long term cell suspension-derived protoplasts ofTriticum aestivum (cv. Jinan 177) and protoplasts ofHaynaldia villosa prepared from one-year-old embryogeneric calli was performed by PEG method. In asymmetric fusion, donor calli were treated with gamma ray at a dose of ... | 18,726,409 |
GABA/progesterone-induced polyphosphoinositide (PPI) breakdown and its role in the acrosome reaction of guinea pig spermatozoa in vitro. | To investigate whether GABA/progesterone (P(4)) stimulates PPI breakdown and its role in the acrosome reaction (AR), spermatozoa of guinea pig were preincubated in MCM-LCa(2+) for 5.5 h and then labeled with [(32)P]pi for 1 h. Samples were washed through a three-step gradient Percoll, adjusted to 5x10(7) cells/mL and e... | 18,726,415 |
Three-dimensional structure of the Chinese Sacbrood bee virus. | The RNA of Chinese Sacbrood Bee Virus (CSBV) was purified and used as template to obtain a 1096 bp cDNA fragment by RT-PCR amplification. This DNA fragment was cloned into pGEM-T Easy Vector for sequencing. Analyses of the sequenced CSBV RNA fragment revealed a nucleotide sequence homology of 87.6% and a deduced amino-... | 18,726,426 |
Laser-induced tobacco protoplast fusion. | Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The result... | 18,726,463 |
Engineering human interferon alpha1c/86D with phage display technology. | Human interferon-alpha1c/86D (IFNalpha1c/86D) was functionally displayed on the surface of the filamentous bacteriophage using a phagemid vector system (pCANTAB5E). The key amino acid residues involved in the receptor binding were further defined with phage displayed 6-mer peptide library and two neutralizing antibodie... | 18,726,473 |
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