title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
|---|---|---|
Impact of preoperative anxiolytic on surgical site infection in patients undergoing abdominal hysterectomy. | An increased anxiety may be associated with a higher risk of surgical site infection (SSI), but there is little objective data on the effect of preoperative anxiolytic interventions on SSI. To address this issue, we evaluated the effects of preoperative diazepam on postoperative SSI following abdominal hysterectomy. Th... | 18,834,731 |
The association between handwashing practices and illness symptoms among college students living in a university dormitory. | We describe handwashing practices, the association of handwashing with upper respiratory and gastrointestinal symptoms, and the effects of gender on handwashing practices among male (n = 215) and female (n = 243) college students. Self-reported frequency of handwashing was not associated with infectious illness symptom... | 18,834,732 |
Religion and culture: potential undercurrents influencing hand hygiene promotion in health care. | Health care-associated infections affect hundreds of millions of patients worldwide each year. The World Health Organization's (WHO) First Global Patient Safety Challenge, "Clean Care is Safer Care," is tackling this major patient safety problem, with the promotion of hand hygiene in health care as the project's corner... | 18,834,738 |
Consideration to culture health care workers related to increased methicillin-resistant Staphylococcus aureus activity in a neonatal intensive care unit. | For 2 consecutive years, a care level III neonatal intensive care unit (NICU) in a 700-bed health care facility experienced an increase in health care-associated methicillin-resistant Staphylococcus aureus (MRSA). To decrease transmission, standard infection prevention techniques, active surveillance cultures, and deox... | 18,834,741 |
Evaluation of patient participation in a patient empowerment initiative to improve hand hygiene practices in a Veterans Affairs medical center. | In patient empowerment initiatives, patients are encouraged to ask caregivers if they have washed their hands. However, relatively little is known about the willingness of patients to participate in such initiatives. Patients were presented with a "Partners in Your Care" script asking them to remind health care workers... | 18,834,750 |
WITHDRAWN: Retrocardiac Sponge-Induced Hemodynamic Instability in the Postcardiopulmonary Bypass Period: Detection by Transesophageal Echocardiography. | The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi:10.1053/j.jvca.2008.11.012. The duplicate article has therefore been withdrawn. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 18,834,782 |
The relationship between carotid blood-flow velocity and the left ventricular area during acute regional ischemia. | Myocardial contractility can be described by the relationship between blood-flow velocity in the carotid artery and the left ventricular cross-sectional area. The authors investigated whether critical myocardial ischemia influences the derived contractility index, E'(es). A prospective animal study. A university resear... | 18,834,794 |
Site-specific labeling of T7 DNA polymerase with a conformationally sensitive fluorophore and its use in detecting single-nucleotide polymorphisms. | Like most enzymes, DNA polymerases undergo a large conformational change on the binding of a correct nucleotide. To determine how the conformational change contributes to substrate specificity, we labeled the T7 DNA polymerase with a conformationally sensitive fluorophore at a position that provides a signal coincident... | 18,834,851 |
Effects of miR-34a on cell growth and chemoresistance in prostate cancer PC3 cells. | Tumor suppressor p53 transcriptionally regulates expression of microRNA-34a, which confers translational inhibition and mRNA degradation of genes involved in cell cycle control and apoptosis. In various cancers, miR-34a expression is lost or reduced. Here, we investigated the role of miR-34a in prostate cancer cell lin... | 18,834,855 |
The cell growth suppressor, mir-126, targets IRS-1. | miRNAs are a family of approximately 22-nuleotide-long noncoding RNAs involved in the formation and progress of tumors. Since traditional methods for the detection of miRNAs expression have many disadvantages, we developed a simple method called polyA RT PCR. With this method, we detected a series of miRNAs and found t... | 18,834,857 |
A high contrast method of unstained biological samples under a thin carbon film by scanning electron microscopy. | The contrast of biological samples in scanning electron microscopy (SEM) is very weak. To examine a biological specimen by SEM, many steps and/or special equipment are required to prepare the sample. Here, we describe a method using an unstained biological sample under a 40 nm carbon film to give a high contrast image,... | 18,834,858 |
Spinophilin inhibits the binding of RGS8 to M1-mAChR but enhances the regulatory function of RGS8. | We showed previously that RGS8 directly binds to the third intracellular loop (i3L) of the M1 muscarinic acetylcholine receptor using the sequence MPRR at the N-terminus of RGS8 and specifically inhibits signal transduction. Here, we identified spinophilin (SPL) as an RGS8-interacting protein. We found that the SPL-bin... | 18,834,863 |
Effects of different extenders on DNA integrity of boar spermatozoa following freezing-thawing. | The sperm-rich fraction, collected from eight mature Yorkshire boars, was frozen in an extender containing 9% LDL (w/v), 100mM trehalose, or 20% yolk (v/v), respectively. Sperm DNA integrity was assessed using the single-cell gel electrophoresis (SCGE). Other sperm quality characteristics such as motility, acrosome and... | 18,834,872 |
Bayesian surprise attracts human attention. | We propose a formal Bayesian definition of surprise to capture subjective aspects of sensory information. Surprise measures how data affects an observer, in terms of differences between posterior and prior beliefs about the world. Only data observations which substantially affect the observer's beliefs yield surprise, ... | 18,834,898 |
Protective effects of IRFI-042 in monensin induced neurotoxicity in chicks. | Monensin, a well known ionophore antibiotic, may cause severe damage in neuronal cells by altering Na+/K+-ATPase and Ca2+-ATPase. We investigated whether IRFI-042, a synthetic analogue of vitamin E, may block lipid peroxidation in neuronal cells and protect against monensin neurotoxicity in chicks. Monensin toxicity wa... | 18,834,916 |
Electroacupuncture decreases nitric oxide synthesis in the hypothalamus of spontaneously hypertensive rats. | Acupuncture-related effects on autonomic function have been explored via biological and neurophysiologic studies. The hypothalamus, known to regulate the autonomic nervous system, is likely affected by acupuncture treatment that modulates sympathetic functions. The aim of this study was to investigate the effect of ele... | 18,834,924 |
Olfactory sensory deprivation increases the number of proBDNF-immunoreactive mitral cells in the olfactory bulb of mice. | In the olfactory bulb, apoptotic cell-death induced by sensory deprivation is restricted to interneurons in the glomerular and granule cell layers, and to a lesser extent in the external plexiform layer, whereas mitral cells do not typically undergo apoptosis. With the goal to understand whether brain-derived neurotrop... | 18,834,927 |
Studies on the immunomodulatory effect of extract of Cyanodon dactylon in shrimp, Penaeus monodon, and its efficacy to protect the shrimp from white spot syndrome virus (WSSV). | The present study investigates the protection of shrimp Penaeus monodon against white spot syndrome virus (WSSV) using antiviral plant extract derived from Cyanodon dactylon and the modulation of the shrimp non-specific immunity. To determine the antiviral activity, the shrimp were treated by both in vitro (intramuscul... | 18,834,943 |
Electrostatically constrained alpha-helical peptide inhibits replication of HIV-1 resistant to enfuvirtide. | Alpha-helical peptides, such as T-20 (enfuvirtide) and C34, derived from the gp41 carboxyl-terminal heptad repeat (C-HR) of HIV-1, inhibit membrane fusion of HIV-1 and the target cells. Although T-20 effectively suppresses the replication of multi-drug resistant HIV variants both in vitro and in vivo, prolonged therapy... | 18,834,950 |
RAD51 foci formation in response to DNA damage is modulated by TIP49. | Chromatin modification plays an important role in modulating the access of homologous recombination proteins to the sites of DNA damage. TIP49 is highly conserved component of chromatin modification/remodeling complexes, but its involvement in homologous recombination repair in mammalian cells has not been examined in ... | 18,834,951 |
DNA methylomes, histone codes and miRNAs: tying it all together. | Our current knowledge of the deregulation that occurs during the onset and progression of cancer and other diseases leads us to recognize both genetic and epigenetic alterations as being at the core of the pathological state. The epigenetic landscape includes a variety of covalent modifications that affect the methylat... | 18,834,952 |
Mutual modulation between interleukin-10 and interleukin-6 induced by Rhodococcus aurantiacus infection in mice. | The interaction between interleukin-10 (IL-10) and interleukin-6 (IL-6) was investigated in the inflammatory response to Rhodococcus aurantiacus (R. aurantiacus) infection, in which both cytokines act as anti-inflammatory cytokines. Compared with wild-type (WT) counterparts, IL-6 gene-deficient (IL-6(-)/(-)) mice mount... | 18,834,949 |
Risk factors for elevated blood lead levels among African refugee children in New Hampshire, 2004. | Surveillance blood lead screening of refugee children resettled in Manchester, NH, in 2004 revealed that 39 (42%) of 92 children had elevated levels (>or=10 microg/dL) after resettlement. Furthermore, 27/92 children (29%) had nonelevated screening blood lead levels on arrival (BLL1) but had elevated follow-up blood ... | 18,834,979 |
Acute arterial thrombosis in the absence of inflammation: the stress-related anti-inflammatory hormone ACTH participates in platelet-mediated thrombosis. | Despite the reciprocal relationship that exists between inflammation and thrombosis, we asked whether thrombosis can develop without inflammation, and whether stress-related hormones (ACTH and cortisol) influence platelet-mediated thrombosis. We investigated the role of ACTH and cortisol in platelet aggregation, as wel... | 18,834,983 |
The pleiotropic effects of statins on endothelial function, vascular inflammation, immunomodulation and thrombogenesis. | Statins have been demonstrated to significantly affect the prognosis and outcome of patients with risk factors to atherosclerosis (in primary and secondary prevention trials). Several clinical and recently basic studies have suggested an extra-beneficial effect of the statins in the prevention of atherosclerosis and co... | 18,834,985 |
Kaolinite flocculation structure. | Effective flocculation and dewatering of mineral processing streams containing colloidal clays has become increasingly urgent. Release of water from slurries in tailings streams and dam beds for recycle water consumption, is usually slow and incomplete. To achieve fast settling and minimization of retained water, indiv... | 18,834,991 |
A 3-O-methylated mannogalactan from Pleurotus pulmonarius: structure and antinociceptive effect. | A polysaccharide (Mw 2.39x10(4)g/mol) was extracted with cold water from the basidiomycete Pleurotus pulmonarius, and its antinociceptive and anti-inflammatory properties were evaluated. It was a mannogalactan (MG), whose structure was characterized using mono- and two-dimensional NMR spectroscopy, methylation analysis... | 18,834,999 |
New Uintan primates from Texas and their implications for North American patterns of species richness during the Eocene. | New omomyid fossils from the Purple Bench locality of the Devil's Graveyard Formation, middle Eocene (Uintan) of southwest Texas, are described. One specimen represents a new genus and species, herein named Diablomomys dalquesti. This new species is allocated to the tribe Omomyini, sister taxon to Omomys and Chumashius... | 18,835,008 |
Identification of white adipocyte progenitor cells in vivo. | The increased white adipose tissue (WAT) mass associated with obesity is the result of both hyperplasia and hypertrophy of adipocytes. However, the mechanisms controlling adipocyte number are unknown in part because the identity of the physiological adipocyte progenitor cells has not been defined in vivo. In this repor... | 18,835,024 |
Tolcapone in elderly patients with Parkinson's disease: a prospective open-label multicenter non-interventional trial. | Safety and efficacy of tolcapone was studied in 237 patients with advanced Parkinson's disease (PD) in a prospective, open-label, multicenter, non-interventional trial. Main outcome measures were tolerability (adverse events=AE) and safety parameters (liver enzymes). A subgroup analysis was performed for patients at &l... | 18,835,049 |
Cutaneous T-cell lymphoma and Staphylococcus aureus colonization. | Bacterial infections are a common complication of cutaneous T-cell lymphoma (CTCL). The most common pathogen of cutaneous infections in CTCL patients is Staphylococcus aureus. The purpose of this study was to assess S aureus colonization rates among CTCL subjects compared to control subjects. Fifty subjects with CTCL, ... | 18,835,065 |
Ferritin for the clinician. | Ferritin, a major iron storage protein, is essential to iron homeostasis and is involved in a wide range of physiologic and pathologic processes. In clinical medicine, ferritin is predominantly utilized as a serum marker of total body iron stores. In cases of iron deficiency and overload, serum ferritin serves a critic... | 18,835,072 |
The neural basis of smooth pursuit eye movements in the rhesus monkey brain. | Smooth pursuit eye movements are performed in order to prevent retinal image blur of a moving object. Rhesus monkeys are able to perform smooth pursuit eye movements quite similar as humans, even if the pursuit target does not consist in a simple moving dot. Therefore, the study of the neuronal responses as well as the... | 18,835,077 |
A model and experimental study for dissolution efficiency of gaseous substrates through in situ sparging. | Delivering electron acceptors, electron donors, and nutrients in gas state has been practiced for in situ bioremediation. A mathematical model based on air-channel concept was developed in this paper to assess the dissolution transient behavior of gaseous substrates for an in situ sparging process using their chemical ... | 18,835,090 |
Gas-liquid hybrid discharge-induced degradation of diuron in aqueous solution. | Degradation of diuron in aqueous solution by gas-liquid hybrid discharge was investigated for the first time. The effect of output power intensity, pH value, Fe(2+) concentration, Cu(2+) concentration, initial conductivity and air flow rate on the degradation efficiency of diuron was examined. The results showed that t... | 18,835,096 |
Nitric oxide removal from flue gas with a biotrickling filter using Pseudomonas putida. | The development of an effective biotrickling filter (BF) system to inoculate a newly isolated strain of Pseudomonas putida SB1 for the effective treatment of nitric oxide (NO) is described. The experiments were carried out in a bench-scale BF under high concentrations of O(2) and NO in simulated flue gas. A method incl... | 18,835,098 |
Magnetic resonance imaging of renal involvement in genetically studied patients with tuberous sclerosis complex. | To evaluate renal involvement in tuberous sclerosis complex (TSC). A series of 24 TSC patients (19 with genetically demonstrated disease), underwent abdominal 1.0-T MR imaging with axial/coronal T1- and T2-weighted scans, with/without fat saturation. We looked for angiomyolipomas (AMLs) and cysts in 47 kidneys of 24 pa... | 18,835,118 |
Preserved awareness of their cognitive deficits in patients with schizophrenia: convergent validity of the SSTICS. | This study aimed to examine the convergent validity of the SSTICS. The association between the SSTICS and the five-factor model of the PANSS was also examined. One hundred and seventy-six schizophrenia-spectrum disorder patients were recruited. A correlation analysis was performed. The SSTICS score correlated with the ... | 18,835,134 |
Pharmacodynamic activity of the lantibiotic MU1140. | This study evaluated the pharmacodynamics of the lantibiotic MU1140 and the ability of selected organisms to develop resistance to this antibiotic. MU1140 demonstrated activity against all Gram-positive organisms tested, including oxacillin- and vancomycin-resistant Staphylococcus aureus and vancomycin-resistant Entero... | 18,835,136 |
Suicidal ideation and time perspective in high school students. | Many studies have enlightened the relevance of deepening our knowledge of suicidal ideation among adolescents. However, research has given insufficient attention to the impact of time perspective on suicidal ideation: the present study confirms this relationship in a large sample of adolescents. A survey was conducted ... | 18,835,138 |
Copper and boron fixation in wood by pyrolytic resins. | A phenol-formaldehyde (PF)-resin designed to penetrate wood and immobilize copper and boron in wood cells for protection against decay was investigated. The phenol portion of the PF-resin was partially substituted with pyrolysis oil derived from softwood bark. The objective was to reduce the environmental impact associ... | 18,835,155 |
NODAPA-OH and NODAPA-(NCS)n: synthesis, 68Ga-radiolabelling and in vitro characterisation of novel versatile bifunctional chelators for molecular imaging. | This report concerns synthesis, (68)Ga-radiolabelling and stability data of 1,4,7-triazacyclononane-1,4-diacetic acid-7-p-isothio-cyanatophenyl-acetic acid (NODAPA-NCS), 1,4,7-triazacyclononane-1-acetic acid-4,7-di-p-isothiocyanatophenyl-acetic acid (NODAPA-(NCS)(2)) and 1,4,7-triazacyclononane-1,4-diacetic acid-7-p-hy... | 18,835,159 |
Quantitative structure-activity relationship studies on 1-aryl-tetrahydroisoquinoline analogs as active anti-HIV agents. | Predictive quantitative structure-activity relationship analysis was developed for a diverse series of recently synthesized 1-aryl-tetrahydroisoquinoline analogs with anti-HIV activities in this study. The conventional 2D-QSAR models were developed by genetic function approximation (GFA) and stepwise multiple linear re... | 18,835,162 |
Non-infective morbidity in diabetic patients undergoing coronary and heart valve surgery. | Studies of postoperative morbidity in diabetics have focussed on infection; however, autonomic and cardiovascular complications of diabetes potentially increase the risk for non-infective morbidity. We sought to investigate major non-infective early postoperative complications in diabetic patients. We identified diabet... | 18,835,187 |
Fas receptor expression in germinal-center B cells is essential for T and B lymphocyte homeostasis. | Fas is highly expressed in activated and germinal center (GC) B cells but can potentially be inactivated by misguided somatic hypermutation. We employed conditional Fas-deficient mice to investigate the physiological functions of Fas in various B cell subsets. B cell-specific Fas-deficient mice developed fatal lymphopr... | 18,835,195 |
Association of increased tracheal mucus accumulation with poor willingness to perform in show-jumpers and dressage horses. | The objective of this study was to examine the association of pharyngeal lymphoid hyperplasia (PLH), recurrent laryngeal neuropathy (RLN), mucus accumulation (MA) score and tracheobronchial secretion neutrophil percentage (TBS-N) with rider-assessed performance in sport horses. Airway endoscopy scores, tracheobronchial... | 18,835,198 |
Imaging of effector memory T cells during a delayed-type hypersensitivity reaction and suppression by Kv1.3 channel block. | Effector memory T (Tem) cells are essential mediators of autoimmune disease and delayed-type hypersensitivity (DTH), a convenient model for two-photon imaging of Tem cell participation in an inflammatory response. Shortly (3 hr) after entry into antigen-primed ear tissue, Tem cells stably attached to antigen-bearing an... | 18,835,197 |
Effects of intermittent high-intensity exercise and carbohydrate supplementation on IGF-1 and glycogen of Wistar rats. | The aim of this study was to analyze the association between glucose infusion during intermittent physical exercise and its metabolic effects on rats. Forty male rats were divided into eight groups based on training (intermittent), exercise and carbohydrate intake (glucose 20%): TEC (trained exercised with carbohydrate... | 18,835,207 |
Challenges to effective crisis management: using information and communication technologies to coordinate emergency medical services and emergency department teams. | The purpose of this study is to identify the major challenges to coordination between emergency department (ED) teams and emergency medical services (EMS) teams. We conducted a series of focus groups involving both ED and EMS team members using a crisis scenario as the basis of the focus group discussion. We also colle... | 18,835,211 |
Quantitative analysis of the effect of porosity on the fatigue strength of bone cement. | This paper reports on the effects of porosity and its distribution on the fatigue strength of bone cement. Hand-mixed (HM) and vacuum-mixed (VM) bone cement samples were fatigue tested to failure. The point of failure commonly coincided with large single pores (in the VM materials) and multiple pores in clusters (in th... | 18,835,229 |
Management of the upper airway and congenital cystic lung diseases in neonates. | Congenital upper airway obstruction can pose difficult problems immediately after birth. Newer strategies to maintain the airway in such situations include pharyngeal ventilation, the laryngeal mask airway and flexible fibreoptic intubation. These methods have decreased the potential for malformations such as Pierre Ro... | 18,835,231 |
Applicability of multigene family-specific antibodies toward studies of the subtilases in Arabidopsis thaliana. | Polyclonal antibodies were made to two synthetic peptides with sequences patterned after conserved regions in a multigene family of 56 subtilisin-related proteolytic enzymes in Arabidopsis thaliana. GST-fusion proteins encompassing full-length or partial cDNAs bearing putative epitope regions were cloned and expressed ... | 18,835,234 |
Large-scale expression, purification, and characterization of an engineered prostacyclin-synthesizing enzyme with therapeutic potential. | Recently, we reported that a novel hybrid enzyme (TriCat enzyme), engineered by linking human cyclooxygenase-2 (COX-2) with prostacyclin (PGI(2)) synthase (PGIS) together through a transmembrane domain, was able to directly integrate the triple catalytic (TripCat) functions of COX-2 and PGIS and effectively convert ara... | 18,835,243 |
Reduced expression of SRC family kinases decreases PI3K activity in NBS1-/- lymphoblasts. | SRC family kinases (SFKs) are involved in the activation of phosphatidylinositol-3-kinase (PI3K). In addition, the activity of this lipid kinase can be regulated by the DNA repair protein NBS1. Here, we describe a disturbed expression of some members of the non-receptor tyrosine kinase family in lymphoblastoid cell lin... | 18,835,245 |
The stigmatization of obesity in children. A survey in Greek elementary schools. | This study investigates elementary school children's prejudice towards obesity. A sample of 1861 underweight, normal, overweight and obese children from Central Greece were instructed to rank 6 drawings of same sex children with various physical disabilities, obesity or no disability at all ("healthy"), according to ho... | 18,835,307 |
Induction of antibody response against hepatitis E virus (HEV) with recombinant human papillomavirus pseudoviruses expressing truncated HEV capsid proteins in mice. | A hepatitis E virus (HEV) vaccine would be valuable to reduce the morbidity and mortality associated with the infection in endemic areas. HEV pseudocapsids and epidermal delivery of HEV ORF2 DNA vaccine by gene-gun have been shown to confer protection against virus challenge in monkeys. Vectorization of a DNA vaccine b... | 18,835,319 |
MG-132 inhibits the TCDD-mediated induction of Cyp1a1 at the catalytic activity but not the mRNA or protein levels in Hepa 1c1c7 cells. | Previous studies have shown that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced degradation of aryl hydrocarbon receptor (AhR) is inhibited by MG-132, a potent inhibitor of the 26S proteasome. Therefore, the current study aims to address the effect of MG-132 on the AhR-regulated gene, cytochrome P450 1a1 (Cyp1a1), ... | 18,835,339 |
Preparation of polysaccharide glassy microparticles with stabilization of proteins. | This study investigates a method of preparing hazard-resistant protein-loaded polysaccharide glassy microparticles using freezing-induced phase separation method without exposure to water/oil, water/air interface and cross-linking reagents. Model protein (such as bovine serum albumin, myoglobin and beta-galactosidase (... | 18,835,346 |
Trace amounts of copper exacerbate beta amyloid-induced neurotoxicity in the cholesterol-fed mice through TNF-mediated inflammatory pathway. | Evidence has been gathered to suggest that trace amounts of copper induce neurotoxicity by interaction with elevated cholesterol in diet. Step-through task and Morris water maze task were used to evaluate cognitive function in the animals. Although a 16-week copper treatment alone in mice showed no significant change i... | 18,835,350 |
Osr1 expression demarcates a multi-potent population of intermediate mesoderm that undergoes progressive restriction to an Osr1-dependent nephron progenitor compartment within the mammalian kidney. | The mammalian metanephric kidney is derived from the intermediate mesoderm. In this report, we use molecular fate mapping to demonstrate that the majority of cell types within the metanephric kidney arise from an Osr1(+) population of metanephric progenitor cells. These include the ureteric epithelium of the collecting... | 18,835,385 |
Protein-protein interactions and lens transparency. | Past studies have identified posttranslational modifications of human lens proteins occurring during cataract formation, and have also demonstrated that protein-protein interactions exist between different lens crystallins. Based upon current theories of lens transparency, these posttranslational modifications and thei... | 18,835,387 |
Contribution of gustation to the palatability of linoleic acid. | We investigated the palatability of a low concentration of linoleic acid (LA) in short-term two-bottle choice tests and licking tests. To examine the contribution of gustation, mice were rendered anosmic with olfactory nerve transection surgery and test solutions were prepared using mineral oil (saturated long-chain hy... | 18,835,400 |
Development of an in vitro test battery for assessing chemical effects on bovine germ cells under the ReProTect umbrella. | Current European legislation for the registration and authorization of chemicals (REACH) will require a dramatic increase in the use of animals for reproductive toxicity testing. Since one objective of REACH is to use vertebrates only as last resort, the development and validation of alternative methods is urgently nee... | 18,835,402 |
Evaluation of proinflammatory cytokine production and liver injury induced by plasmid DNA/cationic liposome complexes with various mixing ratios in mice. | The purpose of this study was to investigate the cytokine production and liver injury induced by lipoplexes prepared with DOTMA/cholesterol and DOTAP/cholesterol liposomes with various mixing ratios in mice. Lipoplexes were prepared with pCMV-Luc and DOTMA/cholesterol or DOTAP/cholesterol liposomes. After intravenous a... | 18,835,442 |
AFM for analysis of structure and dynamics of DNA and protein-DNA complexes. | This paper describes protocols for studies of structure and dynamics of DNA and protein-DNA complexes with atomic force microscopy (AFM) utilizing the surface chemistry approach. The necessary specifics for the preparation of functionalized surfaces and AFM probes with the use of silanes and silatranes, including the p... | 18,835,446 |
Characterisation of chicken Campylobacter jejuni isolates using resolution optimised single nucleotide polymorphisms and binary gene markers. | The principal objective of this study was to determine if Campylobacter jejuni genotyping methods based upon resolution optimised sets of single nucleotide polymorphisms (SNPs) and binary genetic markers were capable of identifying epidemiologically linked clusters of chicken-derived isolates. Eighty-eight C. jejuni is... | 18,835,503 |
Effect of monomeric and oligomeric sugar osmolytes on DeltaGD, the Gibbs energy of stabilization of the protein at different pH values: is the sum effect of monosaccharide individually additive in a mixture? | Thermal denaturation curves of ribonuclease-A were measured by monitoring changes in the far-UV circular dichroism (CD) spectra in the presence of different concentrations of six sugars (glucose, fructose, galactose, sucrose, raffinose and stachyose) and mixture of monosaccharide constituents of each oligosaccharide at... | 18,835,508 |
Prognostic factors after liver resection for hepatocellular carcinoma with hepatitis B virus-related cirrhosis: surgeon's role in survival. | Little is known in judging significant factors that affect the outcome of hepatocellular carcinoma (HCC) patients with hepatitis B virus (HBV)-related cirrhosis undergoing liver resection. The aim of the present study is to investigate the controllable and uncontrollable poor prognostic factors for hepatectomy in patie... | 18,835,514 |
Characterization of ornithine and glutamine lipids extracted from cell membranes of Rhodobacter sphaeroides. | The identification and structural characterization of a series of ornithine lipids extracted from the cell membranes of wild-type Rhodobacter sphaeroides, as well as from a glycerophosphocholine-deficient strain, have been achieved by multistage tandem mass spectrometry of their protonated and deprotonated precursor io... | 18,835,523 |
Three dimensional morphometric analyses of axon terminals early changes induced by methylmercury intoxication in the adult cat striate cortex. | The aim of the present report is to investigate in detail morphometric changes of axon terminals of area 17 of adult cat induced by methylmercury intoxication. Six adult male cats (Felix catus), with 12 h day-light cycle and ad libitum water and food regimen, received a single dose of MeHgCl (6.4 mg/kg) dissolved in mi... | 18,835,550 |
Regulation of root elongation by histone acetylation in Arabidopsis. | Transcriptional repression by histone modification represents a universal mechanism that underlies critical biological processes, such as neurogenesis and hematopoietic differentiation, in animals. In plants, however, the extent to which these regulatory pathways are involved in development and morphogenesis is not wel... | 18,835,563 |
Engagement of TLR signaling as adjuvant: towards smarter vaccine and beyond. | Toll like receptors (TLRs) are a family of conserved pattern recognition receptors that recognizes specific microbial patterns and allow the cell to distinguish between self and non-self materials. The very property of the TLRs to link innate and adaptive immunity offers a novel prospect to develop vaccines engaging TL... | 18,835,576 |
Characterization of the hemoglobins of the Australian lungfish Neoceratodus forsteri (Krefft). | We examined for the first time the hemoglobin components of the blood of the Australian lungfish, Neoceratodus forsteri and their functional responses to pH and the allosteric modulators adenosine triphosphate (ATP), guanosine triphosphate (GTP), 2,3-bisphosphoglyceric acid (BPG) and inositol hexaphosphate (IHP) at 25 ... | 18,835,585 |
Normothermic and hypothermic models for studying the deleterious effects of hypoxia-reoxygenation on EDHF-mediated relaxation in isolated porcine coronary arteries. | The vasomotor response of the coronary artery is altered by hypoxia-reoxygenation (H-R) induced damage. The aim of our study was to compare and evaluate normothermic and hypothermic models which are suitable for future drug studies of vasoprotective action against H-R injury. Porcine coronary arterial rings were isolat... | 18,835,583 |
[De Seze et al. criteria: application to a series of 14 patients presenting a first severe acute demyelinating event]. | The application of de Seze et al. criteria (2007) to patients presenting a first severe acute demyelinating event helps to distinguish acute disseminated encephalomyelitis (ADEM) from other CNS inflammatory diseases, with 83% sensitivity and 95% specificity. We applied these criteria to 14 patients who presented a firs... | 18,835,615 |
Mass balance and isotope effects during nitrogen transport through septic tank systems with packed-bed (sand) filters. | Septic tank systems are an important source of NO3(-) to many aquifers, yet characterization of N mass balance and isotope systematics following septic tank effluent discharge into unsaturated sediments has received limited attention. In this study, samples of septic tank effluent before and after transport through sin... | 18,835,629 |
The masticator space: from anatomy to pathology. | The masticator space is a deep facial space with a complex anatomical structure. The purpose of the present study was to precisely define the masticator space to eliminate the use of obsolete and confusing terms to describe the area, and to illustrate the common mass syndromes. Primary tumors are uncommon, usually beni... | 18,835,644 |
[Uncommon manifestation revealing sarcoidosis]. | Bilateral hilar lymphadenopathy, with or without lung parenchymal infiltrates, is the most common radiographic finding in patients with sarcoidosis. Atypical pulmonary findings have been uncommonly reported and include multiple large lung nodules, cavitation, lobar collapse, pleural effusions or pneumothorax. We report... | 18,835,653 |
A novel approach to reduce hemorrhagic transformation after interventional management of acute stroke: catheter-based selective hypothermia. | Interventional management of acute stroke can significantly increase recanalization rate of the occluded artery, however, this improvement is achieved at the expense of an increased incidence in symptomatic intracranial hemorrhage, which may markedly reduce the therapeutic benefit of this treatment. Hypothermia is one ... | 18,835,666 |
Synchronisation of ovarian follicular waves in the dromedary camel (Camelus dromedarius). | This study was designed to compare the efficacy of various treatments intended to synchronise follicular wave cycles in dromedary camels by removing the existing follicle of unknown size and replacing it with a follicle capable of ovulating at a known time. Camels were randomly assigned to one of five groups and treate... | 18,835,669 |
Cyclodextrin-based potentiometric sensors for midazolam and diazepam. | In this work the implementation of benzodiazepine ion-selective electrodes for pharmaceutical formulations control is described. The solid-contact electrodes for midazolam and diazepam are based on polymeric membranes incorporating respectively beta-cyclodextrin and (2-hydroxiproyl)-gamma-cyclodextrin as ionophores, 2-... | 18,835,673 |
Measuring the quality of substance use disorder treatment: evaluating the validity of the Department of Veterans Affairs continuity of care performance measure. | This study examined the patient- and facility-level associations between the continuity of care performance measure adopted by the Department of Veterans Affairs (VA) and improvements in self-administered Addiction Severity Index (ASI) composites and other indicators of problematic substance use. Up to 50 patients from... | 18,835,678 |
Predictors of opiate agonist treatment retention among injection drug users referred from a needle exchange program. | The aim of this study was to examine the effect of a case management intervention on retention in opiate agonist therapy among injection drug users (IDUs) referred from a needle exchange program (NEP). DESIGN, INTERVENTION, PARTICIPANTS, AND SETTING: A randomized trial of a strengths-based case management intervention ... | 18,835,681 |
Residence time dependent desorption of Staphylococcus epidermidis from hydrophobic and hydrophilic substrata. | Adhesion and desorption are simultaneous events during bacterial adhesion to surfaces, although desorption is far less studied than adhesion. Here, desorption of Staphylococcus epidermidis from substratum surfaces is demonstrated to be residence time dependent. Initial desorption rate coefficients were similar for hydr... | 18,835,700 |
Health and healthcare perspectives of African American residents of an unincorporated community: a qualitative assessment. | Residential perspectives about health in unincorporated communities are virtually unexplored. In this study, we conducted focus groups to assess individual and community health status, environmental health mediators, and systematic barriers to healthcare among African American residents of the unincorporated town, Fres... | 18,835,739 |
High resolution topographical mapping of warm and cold sensitivities. | This study aims to explore the thermal sensitivity distribution (topographical mapping) over the glabrous skin of the hand in males and females. Warm (WT) and cold (CT) thresholds were measured in 25 healthy volunteers (12 females), applying a multi-site test of 23 locations on the volar part of the hand. The palm area... | 18,835,741 |
Immunological commonalities and distinctions between airway and digestive immunity. | Airway and digestive tissues are the frontlines of the body's defense, being continuously exposed to the outside environment and encountering large numbers of antigens and microorganisms. To achieve immunosurveillance and immunological homeostasis in the harsh environments of the mucosal surfaces, the mucosal immune sy... | 18,835,748 |
Influence of subtraction ictal SPECT on surgical management in focal epilepsy of indeterminate localization: a prospective study. | The impact of functional imaging tests on the decision-making and planning process for epilepsy surgery has never been prospectively assessed. We prospectively evaluated 50 consecutively eligible patients whose noninvasive evaluations showed nonlocalized findings and determined how their SISCOM (subtraction ictal SPECT... | 18,835,758 |
Histopathological lesions following intramuscular administration of saline in laboratory rodents and rabbits. | This review was undertaken to assess the nature and incidence of procedure-related changes in mice, rats and rabbits which received saline solution by intramuscular injection. Data were collected on the injection sites from 7 studies representing 152 animals. The original observations by the different study pathologist... | 18,835,765 |
Magnetic resonance imaging of heterotaxis syndrome. | Failure to establish normal left-right body axis (LRA) formation during embryogenesis results in heterotaxis, a multi-malformation syndrome. We report on a 20-year-old young woman who presented to the emergency room with upper abdominal pain. On chest X-ray, dextrocardia was noted. Ultrasound was inconclusive. Barium s... | 18,835,766 |
Tissue processing of nitrite in hypoxia: an intricate interplay of nitric oxide-generating and -scavenging systems. | Although nitrite (NO(2)(-)) and nitrate (NO(3)(-)) have been considered traditionally inert byproducts of nitric oxide (NO) metabolism, recent studies indicate that NO(2)(-) represents an important source of NO for processes ranging from angiogenesis through hypoxic vasodilation to ischemic organ protection. Despite in... | 18,835,812 |
Single-step purification of pepsin-derived monoclonal antibody fragments from crude murine ascitic fluids by ceramic hydroxyapatite high-performance liquid chromatography. | Ceramic hydroxyapatite (CHT) high-performance liquid chromatography (HPLC) is used to purify a variety of classes of monoclonal antibodies (mAbs) from crude murine ascites fluids. We report here that this method is also applicable for simple and efficient purification of many mAb fragments that are generated by pepsin ... | 18,835,826 |
Comparing expression of progesterone and estrogen receptors in testicular tissue from men with obstructive and nonobstructive azoospermia. | The objective of this study was to identify and compare the expression profiles of progesterone receptor (PR) and estrogen receptor alpha (ERalpha) in the testes of men with obstructive azoospermia (OA), maturation arrest (MA), and Sertoli cell-only (SCO) histology. Testicular biopsies were obtained from 10 patients wi... | 18,835,831 |
Association of adiposity status and changes in early to mid-adulthood with incidence of Alzheimer's disease. | Adiposity status and change are potential risk factors for Alzheimer's disease (AD). The authors used data on 2,322 participants in the Baltimore Longitudinal Study of Aging to analyze the relation between AD incidence and adiposity in Cox proportional hazards models, with adjustment for sociodemographic factors and sm... | 18,835,864 |
Accuracy of dementia diagnosis: a direct comparison between radiologists and a computerized method. | There has been recent interest in the application of machine learning techniques to neuroimaging-based diagnosis. These methods promise fully automated, standard PC-based clinical decisions, unbiased by variable radiological expertise. We recently used support vector machines (SVMs) to separate sporadic Alzheimer's dis... | 18,835,868 |
The effect of a 'vanishing twin' on biochemical and ultrasound first trimester screening markers for Down's syndrome in pregnancies conceived by assisted reproductive technology. | Previous studies have found that 1 in 10 in vitro fertilization (IVF) singletons originates from a twin gestation. First trimester Down's syndrome screening markers are altered in assisted reproductive techniques (ART) pregnancies compared with spontaneously conceived pregnancies. The presence of a perished embryo may ... | 18,835,870 |
Fine mapping of the MHC Class III region demonstrates association of AIF1 and rheumatoid arthritis. | The heritability of RA has been estimated to be approximately 55%, of which the MHC contributes about one-third. HLA-DRB1 alleles are strongly associated with RA, but it is likely that significant non-DRB1 MHC genetic susceptibility factors are involved. Previously, we identified two three-marker haplotypes in a 106-kb... | 18,835,879 |
The effect of glycosylation on interparticle interactions and dimensions of native and denatured phytase. | Glycosylation affects the physical properties of proteins in a number of ways including solubility and aggregation behavior. To elucidate the mechanism underlying these effects, we have measured second virial coefficients (A2) of the heavily glycosylated pheniophora lycii phytase (Phy) and its enzymatically deglycosyla... | 18,835,893 |
No impact of protein phosphatases on connexin 43 phosphorylation in ischemic preconditioning. | Cardiac connexin 43 (Cx43) is involved in infarct propagation, and the uncoupling of Cx43-formed channels reduces infarct size. Cx43-formed channels open upon Cx43 dephosphorylation, and ischemic preconditioning (IP) prevents the ischemia-induced Cx43 dephosphorylation. In addition to the sarcolemma, Cx43 is also prese... | 18,835,920 |
Nerve stimulation induced overflow of neuropeptide Y and modulation by angiotensin II in spontaneously hypertensive rats. | The sympathetic nervous system and renin-angiotensin system are both thought to contribute to the development and maintenance of hypertension in experimental models such as the spontaneously hypertensive rat (SHR). We demonstrated that periarterial nerve stimulation (NS) increased the perfusion pressure (PP) and neurop... | 18,835,922 |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.