title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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Advances in magnetic resonance (2008). | Current advances in magnetic resonance, as a diagnostic modality, are discussed in the context of publications from Investigative Radiology during 2007 and 2008. The articles relating to this topic, published during the past 2 years, are reviewed by anatomic region. The discussion concludes with a consideration of magn... | 19,002,061 |
Functional performance following an ice bag application to the hamstrings. | This study examined the immediate and short-term (20 minute) effects of 3- and 10-minute ice bag applications to the hamstrings on functional performance as measured by the cocontraction test, shuttle run, and single-leg vertical jump. Forty-two (25 women, 17 men) recreational or collegiate athletes who were free of in... | 19,002,074 |
[The role of lactoferrin in the iron metabolism. Part I. Effect of lactofferin on intake, transport and iron storage]. | Iron belongs to the most widely distributed elements and is essential for the metabolism of almost all organisms. It is required for enzymatic reactions, in particular of those involving electron transport. It also participates in the transport and storage of oxygen in tissues. Iron is present in hem-containing protein... | 19,002,083 |
Effects of recombinant human activated protein C on the fibrinolytic system of patients undergoing conventional or tight glycemic control. | Recombinant human activated protein C (rh-APC) and tight glycemic control (TGC) have been shown to reduce mortality in septic patients. Both interventions can reduce the plasma concentration and/or activity of the most powerful suppressor of fibrinolysis, plasminogen activator inhibitor-1 (PAI-1). Our aim was to evalua... | 19,002,087 |
Administration of type A botulinum toxin after total hip replacement. | Pain after hip replacement is one of the most common problems during rehabilitation and is often the main obstacle in rehabilitation, even though it can often be controlled by localized cryotherapy and/or administration of analgesics. However, patients with positive anamnesis for hip arthritis and long-lasting pain may... | 19,002,096 |
Etomidate reduces glutamate uptake in rat cultured glial cells: involvement of PKA. | Glutamate is the main excitatory neurotransmitter in the vertebrate CNS. Removal of the transmitter from the synaptic cleft by glial and neuronal glutamate transporters (GLTs) has an important function in terminating glutamatergic neurotransmission and neurological disorders. Five distinct excitatory amino-acid transpo... | 19,002,104 |
Epigenetic regulation of centromeric chromatin: old dogs, new tricks? | The assembly of just a single kinetochore at the centromere of each sister chromatid is essential for accurate chromosome segregation during cell division. Surprisingly, despite their vital function, centromeres show considerable plasticity with respect to their chromosomal locations and activity. The establishment and... | 19,002,142 |
Signaling cross-talk between TGF-beta/BMP and other pathways. | Transforming growth factor-beta (TGF-beta)/bone morphogenic protein (BMP) signaling is involved in the vast majority of cellular processes and is fundamentally important during the entire life of all metazoans. Deregulation of TGF-beta/BMP activity almost invariably leads to developmental defects and/or diseases, inclu... | 19,002,158 |
Gene-mediated restoration of normal myofiber elasticity in dystrophic muscles. | Dystrophin mediates a physical link between the cytoskeleton of muscle fibers and the extracellular matrix, and its absence leads to muscle degeneration and dystrophy. In this article, we show that the lack of dystrophin affects the elasticity of individual fibers within muscle tissue explants, as probed using atomic f... | 19,002,166 |
Concepts of epigenetics in prostate cancer development. | Substantial evidence now supports the view that epigenetic changes have a role in the development of human prostate cancer. Analyses of the patterns of epigenetic alteration are providing important insights into the origin of this disease and have identified specific alterations that may serve as useful diagnostic and ... | 19,002,169 |
Isothiocyanate NB7M causes selective cytotoxicity, pro-apoptotic signalling and cell-cycle regression in ovarian cancer cells. | The present report identifies indole-3-ethyl isothiocyanate NB7M as a potent cytotoxic agent with selective activity against cell lines derived from various tumour types. Ovarian cancer cell lines showed sensitivity to NB7M (60-70% cytotoxicity at 2.5 microM), in contrast to control cells (TCL-1 and HTR-8; IC(50) appro... | 19,002,174 |
Roles of macrophages in flow-induced outward vascular remodeling. | Sustained hemodynamic stresses, especially sustained high blood flow, result in flow-induced outward vascular remodeling. Mechanisms that link hemodynamic stresses to vascular remodeling are not well understood. Inflammatory cells, known for their release of proteinases, including matrix metalloproteinases (MMPs), are ... | 19,002,198 |
Bioelectrical impedance phase angle in constitutionally lean females, ballet dancers and patients with anorexia nervosa. | Several articles have described body composition in anorexia nervosa, whereas little is known about this issue in underweight ballet dancers and constitutionally lean females. The main aim of this study was to assess whether phase angle (a bioimpedance variable related to body cell mass) differs according to the type o... | 19,002,201 |
Beam deflection and scanning by two-mirror and two-axis systems of different architectures: a unified approach. | An analytical model is developed to predict the structures of the scan fields generated by two-mirror-two-axis beam scanning systems of different architectures, including (1) two oscillating galvanometric scanners, (2) the paddle scanner two-mirror system, and (3) the golf club two-mirror system. It is found that the s... | 19,002,221 |
Compensating the intensity fall-off effect in cone-beam tomography by an empirical weight formula. | The Feldkamp-David-Kress (FDK) algorithm is widely adopted for cone-beam reconstruction due to its one-dimensional filtered backprojection structure and parallel implementation. In a reconstruction volume, the conspicuous cone-beam artifact manifests as intensity fall-off along the longitudinal direction (the gantry ro... | 19,002,227 |
Absolute testing of the reference surface of a Fizeau interferometer through even/odd decompositions. | Absolute testing of spherical surfaces is a technological necessity because of increased accuracy requirements. In a Fizeau setup, the main part of the interferometer deviations thereby comes from the reference surface. We demonstrate the validity of an absolute testing procedure for the reference surface that has been... | 19,002,239 |
Correlative and dynamic imaging of the hatching biology of Schistosoma japonicum from eggs prepared by high pressure freezing. | Schistosome eggs must traverse tissues of the intestine or bladder to escape the human host and further the life cycle. Escape from host tissues is facilitated by secretion of immuno-reactive molecules by eggs and the formation of an intense strong granulomatous response by the host which acts to exclude the egg into g... | 19,002,240 |
Wound-induced endogenous jasmonates stunt plant growth by inhibiting mitosis. | When plants are repeatedly injured their growth is stunted and the size of organs such as leaves is greatly reduced. The basis of this effect is not well-understood however, even though it reduces yield of crops injured by herbivory, and produces dramatic effects exemplified in ornamental bonsai plants. We have investi... | 19,002,244 |
Campylobacter infection as a trigger for Guillain-Barré syndrome in Egypt. | Most studies of Campylobacter infection triggering Guillain-Barré Syndrome (GBS) are conducted in western nations were Campylobacter infection and immunity is relatively rare. In this study, we explored Campylobacter infections, Campylobacter serotypes, autoantibodies to gangliosides, and GBS in Egypt, a country where ... | 19,002,255 |
Genome-scale validation of deep-sequencing libraries. | Chromatin immunoprecipitation followed by high-throughput (HTP) sequencing (ChIP-seq) is a powerful tool to establish protein-DNA interactions genome-wide. The primary limitation of its broad application at present is the often-limited access to sequencers. Here we report a protocol, Mab-seq, that generates genome-scal... | 19,002,256 |
Plasma gelsolin depletion and circulating actin in sepsis: a pilot study. | Depletion of the circulating actin-binding protein, plasma gelsolin (pGSN) has been described in septic patients and animals. We hypothesized that the extent of pGSN reduction correlates with outcomes of septic patients and that circulating actin is a manifestation of sepsis. We assayed pGSN in plasma samples from non-... | 19,002,257 |
Viscous instabilities in flowing foams: a Cellular Potts Model approach. | The Cellular Potts Model (CPM) successfully simulates drainage and shear in foams. Here we use the CPM to investigate instabilities due to the flow of a single large bubble in a dry, monodisperse two-dimensional flowing foam. As in experiments in a Hele-Shaw cell, above a threshold velocity the large bubble moves faste... | 19,002,269 |
A guided abstinence experience to illustrate addiction recovery principles. | To develop and implement an elective pharmacy course that included a guided abstinence experience to illustrate addiction recovery principles. A 1-credit elective course to illustrate addiction recovery principles was developed and implemented. The course required students to give up a habit for 6 weeks that was causin... | 19,002,278 |
Bugs and the big bang. | Now that's a cheery thought! Somewhere more than 100 km below the Geneva countryside two parallel beams of subatomic particles are whizzing around a 27 km circuit in opposite directions at about 99% of the speed of light, doing over 11 000 laps per second. Physicists hope to create a 'bang' that won't end the world, bu... | 19,002,295 |
Red flags in scleroderma. | Scleroderma (systemic sclerosis) is an uncommon connective tissue disease characterised by vascular, inflammatory and fibrotic dysfunction of multiple organ systems. Systemic sclerosis is often recognised late in the course of the disease. This article outlines the clinical features of systemic sclerosis, in particular... | 19,002,303 |
Trapped in the net? What to look for in a web based CPD program. | Increasing numbers of general practitioners access web based education programs for continuing professional development (CPD). Web based programs vary in style, content, relevance, reliability, authorship and sponsorship, and hence educational quality. This article outlines how to choose a web based CPD program. Busy G... | 19,002,305 |
Desire for research in general practice. | If you have considered participating in general practice research, the chances are that a combination of inherent interest and potential satisfaction drew you toward it. You may want to see the evidence base of general practice expanded; you probably enjoy an intellectual challenge; and you are likely to take pleasure ... | 19,002,311 |
GP management of osteoarthritic pain in Hong Kong. | Limited information is available about general practitioners' knowledge, attitude and practice in treating osteoarthritis. This study sought to better understand GPs' management of mild to moderate osteoarthritis in Hong Kong. A cross sectional survey of GPs using a structured questionnaire mailed in three stages. A to... | 19,002,312 |
Rh(I) and Ir(I) catalysed intermolecular hydroamination with substituted hydrazines. | The catalysed intermolecular hydroamination of a series of terminal alkynes with substituted hydrazines was achieved using Rh(I) and Ir(I) complexes. | 19,002,322 |
Sweet's syndrome: a retrospective clinical, histopathological and immunohistochemical analysis of 11 cases. | The aim of this paper is to report our clinical experience of Sweet's syndrome, a severe dermatological disease which may be extremely important to recognize for the early diagnosis of a neoplastic disorder. Eleven patients affected by Sweet's syndrome, treated at the Department of Dermatology, University of Ferrara, F... | 19,002,347 |
A modification of Labhardt's high perineoplasty for treatment of pelvic organ prolapse in the very old. | A new simple colpocleisis technique is introduced for the treatment of pelvic organ prolapse in the very old acoital patient with extensive co-morbidity and enhanced intra-operative risk. The method is described and can be performed under local anesthesia. Main outcome measure was patient's satisfaction. Thirty patient... | 19,002,368 |
Analysis of intracranial pressure changes during early versus late percutaneous tracheostomy in a neuro-intensive care unit. | We aimed to investigate intracranial pressure (ICP) changes during early versus late bedside percutaneous tracheostomy (PT) in a neuro-intensive care unit (NICU). This study included 30 patients admitted to our NICU for head trauma, subarachnoid haemorrhage, intracerebral haematoma or brain tumour with a Glasgow Coma S... | 19,002,373 |
Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis. | Coenzyme Q is a lipid molecule required for respiration and antioxidant protection. Q biosynthesis in Saccharomyces cerevisiae requires nine proteins (Coq1p-Coq9p). We demonstrate in this study that Q levels are modulated during growth by its conversion from demethoxy-Q (DMQ), a late intermediate. Similar conversion wa... | 19,002,377 |
Calcium, magnesium, iron, zinc and copper concentration in the hair of tobacco smokers. | In the research, the content of bioelements (Ca, Mg, Fe, Zn, and Cu) in the hair of individuals who reside in similar environmental conditions was spectrometrically determined with the use of AES-ICP spectrophotometer. The relation to their tobacco-smoking habit, age, and sex was established. It was observed that the r... | 19,002,389 |
Biodegradation of crosslinked acrylic polymers by a white-rot fungus. | Two synthetic superabsorbent crosslinked acrylic polymers were mineralized by the white-rot fungus Phanerochaete chrysosporium. The amount of polymer converted to CO(2) increased as the amount of polymer added to the cultures increased. In the presence of sufficiently large amounts of the superabsorbents, such that all... | 19,002,412 |
[Positioning of the patient for surgery]. | The success of an operation does not only depend on a perfect surgical technique, an appropriate anesthesia, convenient surgical instruments and functional technical equipment, but also on a proper operative positioning. Meeting the requirements of the surgeon, the positioning has also to be in accordance with the pati... | 19,002,419 |
IL-1beta-induced chemokine and Fas expression are inhibited by suppressor of cytokine signalling-3 in insulin-producing cells. | Chemokines recruit activated immune cells to sites of inflammation and are important mediators of insulitis. Activation of the pro-apoptotic receptor Fas leads to apoptosis-mediated death of the Fas-expressing cell. The pro-inflammatory cytokines IL-1beta and IFN-gamma regulate the transcription of genes encoding the F... | 19,002,429 |
Effects of gabapentin and pregabalin on K+-evoked 3H-GABA and 3H-glutamate release from human neocortical synaptosomes. | One site of action of the anticonvulsant, analgesic, and anxiolytic drugs gabapentin and pregabalin is the alpha(2)delta-subunit of voltage-sensitive Ca(2+) channels (VSCC). We therefore analyzed the effects of gabapentin and pregabalin on K(+)-evoked release of (3)H-gamma-aminobutyric acid (GABA) and (3)H-glutamate fr... | 19,002,437 |
Capillary waveguide fluoroimmunosensor with improved repeatability and detection sensitivity. | An optical capillary waveguide fluoroimmunosensor based on glass capillaries internally coated with an ultrathin poly(dimethylsiloxane) (PDMS) film is presented. The evaluation of the capillaries developed was done in comparison with aminosilanized [3-(aminopropyl)triethoxysilane, APTES] glass and poly(methylpentene) (... | 19,002,440 |
Strategies for PHA production by mixed cultures and renewable waste materials. | Production of polyhydroxyalkanoates (PHA) by mixed cultures has been widely studied in the last decade. Storage of PHA by mixed microbial cultures occurs under transient conditions of carbon or oxygen availability, known respectively as aerobic dynamic feeding and anaerobic/aerobic process. In these processes, PHA-accu... | 19,002,455 |
The liver and kidney expression of sulfate anion transporter sat-1 in rats exhibits male-dominant gender differences. | The sulfate anion transporter (sat-1, Slc26a1) has been cloned from rat liver, functionally characterized, and localized to the sinusoidal membrane in hepatocytes and basolateral membrane (BLM) in proximal tubules (PT). Here, we confirm previously described localization of sat-1 protein in rat liver and kidneys and rep... | 19,002,488 |
Are the birch trees in Southern England a source of Betula pollen for North London? | Birch pollen is highly allergenic. Knowledge of daily variations, atmospheric transport and source areas of birch pollen is important for exposure studies and for warnings to the public, especially for large cities such as London. Our results show that broad-leaved forests with high birch tree densities are located to ... | 19,002,505 |
Pain palliation in patients with bone metastases using MR-guided focused ultrasound surgery: a multicenter study. | Noninvasive thermal ablation using magnetic resonance (MR)-guided focused ultrasound (MRgFUS) has been shown to be clinically effective in uterine fibroids, and is being evaluated for ablation of breast, liver, and brain lesions. Recently MRgFUS has been evaluated for palliation of pain caused by bone metastases. We pr... | 19,002,530 |
Surgical site infections following colorectal surgery in patients with diabetes: association with postoperative hyperglycemia. | Postoperative glycemic control reduces sternal infections following cardiac surgery in patients with diabetes mellitus (DM). The objective of this study was to examine the relationship between postoperative glycemic control and surgical site infections (SSI) in patients with DM undergoing colorectal resection. A cohort... | 19,002,535 |
The regulation of medical malpractice in Japan. | How Japanese legal and social institutions handle medical errors is little known outside Japan. For almost all of the 20th century, a paternalistic paradigm prevailed. Characteristics of the legal environment affecting Japanese medicine included few attorneys handling medical cases, low litigation rates, long delays, p... | 19,002,542 |
Targeted delivery of PSC-RANTES for HIV-1 prevention using biodegradable nanoparticles. | Nanoparticles formulated from the biodegradable co-polymer poly(lactic-co-glycolic acid) (PLGA), were investigated as a drug delivery system to enhance tissue uptake, permeation, and targeting for PSC-RANTES anti-HIV-1 activity. PSC-RANTES nanoparticles formulated via a double emulsion process and characterized in both... | 19,002,569 |
Primary immunodeficiency diseases in Egyptian children: a single-center study. | Sixty-four primary immunodeficiency patients were registered at the Pediatric Allergy and Immunology Department, Children's Hospital, Ain Shams University, Cairo, Egypt. Predominantly antibody deficiencies were the most common category (35.9%) followed by combined T- and B-cell immunodeficiencies (29.7%), other well de... | 19,002,574 |
Oscillatory perfusion culture of CaP-based tissue engineering bone with and without dexamethasone. | Dexamethasone, a powerful osteogenic agent for osteoblast differentiation, has been suggested to have synergistic effects when applied together with perfusion culture. As ceramic scaffolds are widely used clinically and oscillatory flow well replicates the natural physical conditions, the biological effects of dexameth... | 19,002,585 |
Transport and dynamics of toxic pollutants in the natural environment and their effect on human health: research gaps and challenge. | The source-pathway-receptor (SPR) approach to human exposure and risk assessment contains considerable uncertainty when using the refined modelling approaches to pollutant transport and dispersal, not least in how compounds of concern might be prioritized, proxy or indicator substances identified and the basic environm... | 19,002,593 |
Progress in herbicide determination with the thylakoid bioassay. | Chloroplast thylakoids are used as biological units to determine herbicides in different kinds of water samples as well as in aqueous extracts of compost, soil or food samples. The thylakoid bioassay shows clearly inhibition of fluorescence yield in the presence of photosystem II specific herbicides. Due to this method... | 19,002,623 |
Toxicity testing of heavy metals with the Rhizobium-legume symbiosis: High sensitivity to cadmium and arsenic compounds. | Legume root nodules are the site of biological nitrogen fixation in the Rhizobium-legume symbiosis. Nodules are structures unique to this symbiosis and they are morphologically as well as physiologically distinct from other plant organs. Organic substances affecting the macro- or microsymbionts vitality, such as PAHs (... | 19,002,625 |
Distribution of 14C-TNT and derivatives in different biochemical compartments of Phaseolus vulgaris. | (14)C-TNT was used to quantify the uptake rate and metabolic turnover of TNT in Phaseolus vulgaris. Seventeen plants were analysed by a special cell fractionation method with polar and nonpolar solvents and enzymes. We obtained three cytoplasmic fractions and five cell wall derived fractions. The recovery rate was 72% ... | 19,002,633 |
Sources of anthropogenic platinum-group elements (PGE): Automotive catalysts versus PGE-processing industries. | Soil samples from the area of Hanau (Hessen, Germany) were analyzed for anthropogenic platinum-group elements (PGE). The results confirm the existence of two different sources for anthropogenic PGE: 1. automotive catalysts, and 2. PGE-processing plants. Both sources emit qualitatively and quantitatively different PGE s... | 19,002,636 |
Pharmacotherapy of urinary incontinence. | The pathophysiology of urinary incontinence (UI) involves an overactive detrusor (DOA) or an incompetent urethral sphincter. Therefore, the three most common types of UI are stress urinary incontinence (SUI), urge urinary incontinence (UUI), or a combination of stress and urge known as mixed urinary incontinence (MUI).... | 19,002,644 |
Improved ethanol production by mixed immobilized cells of Kluyveromyces marxianus and Saccharomyces cerevisiae from cheese whey powder solution fermentation. | Ethanol productions from cheese whey powder (CWP) solution were investigated by using free or immobilized cells of Kluyveromyces marxianus in monocultures or mixed cultures with free or immobilized cells of K. marxianus and Saccharomyces cerevisiae. K. marxianus free cells produced 3.8% v/v ethanol in monocultures, whi... | 19,002,658 |
[Effective acquisition of basic surgical techniques through blended learning]. | Large student numbers and heterogeneous teaching pools hamper standardized teaching and impede objective assessment of surgical skills. This article presents the advantages of new teaching media in a "blended learning" concept for training surgical skills to medical students at the Basel University Medical School in Sw... | 19,002,661 |
An analysis of outcome of arthroscopic versus mini-open rotator cuff repair using subjective and objective scoring tools. | The purpose is to perform a comparative analysis of mini-open and arthroscopic rotator cuff repairs through the use of subjective and objective scoring tools. We conducted a prospective comparative cohort study that evaluated 123 consecutive patients who underwent rotator cuff repairs (arthroscopic and 31 mini-open rep... | 19,002,667 |
How critical is the use of commercially available enzymes for selenium speciation? | The aim of this work was to check whether commercially available enzymes are pure enough to be used for selenium speciation analysis and the contribution that impurities could make to Se determination in real samples. For this purpose, twelve commercially available enzymes with different origins and classifications (pr... | 19,002,673 |
Does reflux in orthotopic diversion matter? A randomized prospective comparison of the Studer and T-pouch ileal neobladders. | Orthotopic neobladder reconstruction has become a standard form of urinary diversion in many centers for patients undergoing radical cystectomy for bladder cancer. There is still controversy about the best technique for construction of the neobladder, and especially whether it is necessary to include an antireflux mech... | 19,002,689 |
Carbon dioxide does not affect the methylation status of prognostic important oncogenes Rassf1A and DCR2 in neuroblastoma cells. | The aim of the current study was to investigate effects of CO(2) atmosphere, mimicking conditions of the pneumoperitoneum during laparoscopy, on epigenetic conditions of Rassf1A and DCR2 oncogenes in neuroblastoma cells. SH-SY5Y neuroblastoma cells were exposed to 100% CO(2) for 4 h. Cells were lysed 4, 8 and 168 h aft... | 19,002,696 |
Spiral CT virtual bronchoscopy with multiplanar reformatting in the evaluation of post-intubation tracheal stenosis: comparison between endoscopic, radiological and surgical findings. | We evaluated the usefulness and accuracy of spiral CT in detection and assessment of post-intubation tracheal stenosis. Fourteen patients with post intubation stenosis underwent evaluation of their airway by spiral CT scan with multiplanar reformatting (MPR) and virtual endoscopy (VE) and conventional rigid bronchoscop... | 19,002,699 |
The effect of 60-h sleep deprivation on cardiovascular regulation and body temperature. | This study examined cardiovascular regulation and body temperature (BT) during 60 h of sleep deprivation in 20 young healthy cadets. Heart rate variability was measured during an active orthostatic test (AOT). Measurements were performed each day in the morning and evening after 2, 14, 26, 38, 50 and 60 h of sleep depr... | 19,002,705 |
The evolution of pyrroline-5-carboxylate synthase in plants: a key enzyme in proline synthesis. | Many plants synthesize and accumulate proline in response to osmotic stress conditions. A central enzyme in the proline biosynthesis is the bifunctional enzyme Delta(1)-pyrroline-5-carboxylate synthase (P5CS) that includes two functional catalytic domains: the gamma-glutamyl kinase and the glutamic-gamma-semialdehyde d... | 19,002,717 |
Significance of hemolysis on extracorporeal life support after cardiac surgery in children. | Hemolysis is common during extracorporeal life support (ECLS). Elevated levels of circulating plasma free hemoglobin (FHb) has been linked to the development of hemoglobinuria nephropathy. Its clinical significance in patients receiving ECLS remains unknown. Medical records of 104 children <3 years old who required ... | 19,002,722 |
Obesity surgery results depending on technique performed: long-term outcome. | Many techniques have excellent results at 2 years of follow-up but some matters regarding their long-term efficacy have arisen. This is why bariatric surgery results must be analyzed in long-term follow-up. The aim of this study was to extend the analysis over 5 years, evaluating weight loss, morbidity, and mortality o... | 19,002,740 |
Large-cell neuroendocrine carcinoma without immunohistological positivity or serum elevation of CEA until relapse. | We herein report a case of large cell neuroendocrine carcinoma (LCNEC) originating in the right lung upper lobe and showing unique features at relapse in the right middle lobe. The relapsed tumor pathology included a carcinoembryonic antigen (CEA)-positive mantle component and a CEA-negative core area. The latter showe... | 19,002,754 |
Hydrogen production and anaerobic decolorization of wastewater containing Reactive Blue 4 by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa. | Anaerobic biodegradability of wastewater (3,000 mg CODcr/l) containing 300 mg/l Reactive Blue 4, with different co-substrates, glucose, butyrate and propionate by a bacterial consortium of Salmonella subterranea and Paenibacillus polymyxa, concomitantly with hydrogen production was investigated at 35 degrees C. The acc... | 19,002,762 |
Prognostic potential of precise molecular diagnosis of Autosomal Recessive Osteopetrosis with respect to the outcome of bone marrow transplantation. | Hematopoietic stem cell transplantation (HSCT) is often the only practical approach to fatal genetic defects. One of the first pathologies which HSCT was applied to was Autosomal Recessive Osteopetrosis (ARO), a rare genetic bone disease in which a deficit in bone resorption by osteoclasts leads to increased bone densi... | 19,002,772 |
Drug resistance and DNA repair in leukaemia. | Most cytotoxic agents exert their action via damage of DNA. Therefore, the repair of such lesions is of major importance for the sensitivity of malignant cells to chemotherapeutic agents. The underlying mechanisms of various DNA repair pathways have extensively been studied in yeast, bacteria and mammalian cells. Sensi... | 19,002,791 |
Properties of ras-amplified recombinant BHK-21 cells in protein-free culture. | We compared serum and protein-free cultures ofa ras-amplified recombinant BHK-21 cell line(ras-rBHK-IgG), which hyperproduces a lungcancer specific recombinant human monoclonal antibody. Ras-rBHK-IgG cells were shown to grow well, evenin protein-free medium and to be morphologicallysimilar to cells cultured in serum co... | 19,002,807 |
Improvement of a method to reproducibly immortalize human T cells by oncogene transfection. | The method to immortalize human T cells efficiently and reproduciblyby oncogene transfection was improved. T cells were first grown selectively from peripheralblood lymphocytes population of healthy donors andatopic asthma patients, and from lymph nodelymphocytes population of lung cancer patients byactivating with mit... | 19,002,813 |
Micropattern-immobilization of heparin to regulate cell growth with fibroblast growth factor. | Heparin was immobilized on a polystyrene plate in a specificpattern by photolithography. Heparin was coupled with azidoaniline. Thederivatized heparin was cast on the polystyrene plate from aqueoussolution. After drying, the plate was photo-irradiated in the presence of aphotomask. The micropatterning was confirmed by ... | 19,002,818 |
Treatment of mouse melanoma cells with phorbol 12-myristate 13-acetate counteracts mannosylerythritol lipid-induced growth arrest and apoptosis. | Mannosylerythritol lipid (MEL), an extracellularglycolipid from yeast, induces the differentiation ofHL-60 promyelocytic leukemia cells towardsgranulocytes. We show here that MEL is also a potentinhibitor of the proliferation of mouse melanoma B16cells. Flow-cytometric analysis of the cell cycle ofMEL-treated B16 cells... | 19,002,819 |
Glutamate triggers elevation of intracellular Ca(2+) concentration in neural precursor cells. | Both neurons and glial cells are derived from neuralprecursor cells in the ventricular zone during braindevelopment. The fate of the neural precursor cells isaffected by neurotransmitters such as glutamate. Inthis study, we examined glutamate-triggeredintracellular Ca(2+) signaling in neural precursorcell lines by the ... | 19,002,823 |
Separation of CHO cells using hydrocyclones. | Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It ... | 19,002,842 |
The effects of boric acid on sister chromatid exchanges and chromosome aberrations in cultured human lymphocytes. | The aim of this study was to determine the possible genotoxic effects of boric acid (BA) (E284), which is used as an antimicrobial agent in food, by using sister chromatid exchange (SCEs) and chromosome aberration (CAs) tests in human peripheral lymphocytes. The human lymphocytes were treated with 400, 600, 800, and 10... | 19,002,846 |
Small interfering RNA knockdown of mini-TyrRS and mini-TrpRS effects angiogenesis in human umbilical vein endothelial cells in hypoxic culture. | Aim We studied the role of mini-TyrRS and mini-TrpRS in angiogenesis by using small interfering RNA-mediated mini-TyrRS/mini-TrpRS knockout in hypoxic culture of human umbilical vein endothelial cells. Methods SiRNA was used as the main method to inhibited the gene function. Silencing efficiency was assayed by real-tim... | 19,002,860 |
Related effects of cell adaptation to serum-free conditions on murine EPO production and glycosylation by CHO cells. | The necessity to perform serum-free cultures to produce recombinant glycoproteins generally requires an adaptation procedure of the cell line to new environmental conditions, which may therefore induce quantitative and qualitative effects on the product, particularly on its glycosylation. In previous studies, desialyla... | 19,002,864 |
Influence of medium type and serum on MTT reduction by flavonoids in the absence of cells. | The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay is widely accepted as a simple and reproducible method for determining cell proliferation or cytotoxicity in vitro. In this study, we show that the flavonoids quercetin, rutin and luteolin but not apigenin can reduce MTT in the absence of liv... | 19,002,877 |
In vitro immunization can elicit the expansion of diverse repertoire of B cells from peripheral blood mononuclear cells. | We previously developed an in vitro immunization (IVI) protocol of human peripheral blood mononuclear cells (PBMC) for generating antigen-specific human antibodies. In order to clarify whether IVI protocolinduces antigen-specific B cell responses in PBMC, we analyzed family gene usage and sequence of the variable regio... | 19,002,881 |
Culture of bovine hepatocytes: a non-perfusion technique for cell isolation. | In this work we have studied the isolation and culture of mature bovine hepatocytes on plastic dishes without exogenous matrix. The liver has been disaggregated in a collagenase solution instead of undergoing a perfusion step. After a few days in culture, the plates showed several clusters of different cell types. Alth... | 19,002,895 |
Caspase activation, sialidase release and changes in sialylation pattern of recombinant human erythropoietin produced by CHO cells in batch and fed-batch cultures. | The activation of caspases represents a crucial turning point during a batch or a fed-batch culture of mammalian cells. It not only affects the quantity but also the quality of the recombinant glycoprotein produced. In this study, the activation of various caspases, the release of intracellular sialidase and the change... | 19,002,897 |
Pooling and PCR as a method to combat low frequency gene targeting in mouse embryonic stem cells. | The introduction of germ line modifications by gene targeting in mouse embryonic stem (ES) cells has proven a fundamental technology to relate genes to mammalian biology. Critical aspects required for successful gene targeting have traditionally been experimental enhancements that increase the frequency or detection of... | 19,002,898 |
Amino acids metabolism by VO 208 hybridoma cells: some aspects of the culture process and medium composition influence. | In the present study an approach has been developed in order to examine the consequence of essential and non essential amino acid supplementation on VO208 hybridoma cells behaviour. The effect of amino acid enrichment has been studied taking into account the culture process, i.e., batch or continuous culture mode and t... | 19,002,906 |
Stable expression of recombinant human coagulation factor XIII in protein-free suspension culture of Chinese hamster ovary cells. | The recombinant a and bsubunits for human coagulation factor XIII were transfected into Chinese hamster ovary (CHO) cells. CHO cells were amplified and selected with methotrexate in adherent cultures containing serum, and CHO 1-62 cells were later selected in protein-free medium. To develop a recombinant factor XIII pr... | 19,002,921 |
Production of interferon-beta by NB1-RGB cells cultured on peptide-lipid membranes. | Cell growth and production of interferon-beta (IFN-beta) were investigated for normal human skin fibroblast cells (NB1-RGB) cultured on membranes prepared from peptide-lipids containing the arginine-glycine-aspartic acid [Arg-Gly-Asp] (RGD), tyrosine-isoleucine-glycine-serine-arginine [Tyr-Ile-Gly-Ser-Arg] (YIGSR) and ... | 19,002,924 |
SEAP expression in transiently transfected mammalian cells grown in serum-free suspension culture. | A transient transfection process was established using a novel 'in-house' developed transfection reagent, Ro-1539. It allows rapid production of large quantities of various recombinant proteins. Here we describe the transient expression of the secreted human placental alkaline phosphatase (SEAP) by HEK293EBNA and CHO c... | 19,002,927 |
Development of a standardized protocol for reproducible generation of matured monocyte-derived dendritic cells suitable for clinical application. | There is increasing interest in the generation of dendritic cells (DC) for cancer immunotherapy. In order to utilize DC in clinical trials it is necessary to have standardized, reproducible and easy to use protocols. We describe here the process development for the generation of DC as the result of investigation of cul... | 19,002,934 |
Use of hollow fiber systems for rapid and direct scale up of antibody production from hybridoma cell lines cultured in CL-1000 flasks using BD Cell MAb medium. | The combination of BD Cell MAb medium with the CL-1000 flask is increasingly being used to generate a few hundred milligram of antibody for early stage research projects. Cells are inoculated at 2 million per ml, and the antibody is harvested after 15 days or when the antibody concentration reaches above 10 mg ml(-1), ... | 19,002,937 |
Estimating the number of viable animal cells in multi-well cultures based on their lactate dehydrogenase activities. | A method is described for estimating the numbers ofanimal cells in multi-well culture by simultaneouslymeasuring the lactate dehydrogenase activity of thetotal culture and the medium. The difference betweenthe two reflects the dehydrogenase content of thecells and correlates with cell number. This LDH/INTmethod was tes... | 19,002,967 |
Matrigel increases the rate of split wound healing and promotes keratinocyte ;take' in deep wounds in rats. | The influence of matrigel, a mixture of the components of thebasement membrane, on the wound healing was studied in a modelof experimental wounds in rats. Matrigel was found to increasethe rate of epithelization of split-thickness wounds. The modelof deep wound was developed in which the host animal could notprovide en... | 19,002,969 |
Manipulation of cells through elastic films. | Manipulation of cells in open dishes is often incompatible withpreservation of sterility. A dish covered with an elastic orstretchable latex or plastic film allows manipulation of cellsthrough the film with preservation of sterility of the cultureand the integrity of the film. The latter forms a ;microglove'for the ins... | 19,002,987 |
Manufacture of a human mesenchymal stem cell population using an automated cell culture platform. | Tissue engineering and regenerative medicine are rapidly developing fields that use cells or cell-based constructs as therapeutic products for a wide range of clinical applications. Efforts to commercialise these therapies are driving a need for capable, scaleable, manufacturing technologies to ensure therapies are abl... | 19,002,992 |
Recent advances in the generation of human monoclonal antibody. | The use of monoclonal antibodies (mAbs) has now gained a niche as an epochal breakthrough in medicine. Engineered antibodies (Abs) currently account for over 30% of biopharmaceuticals in clinical trials. Several methods to generate human mAbs have evolved, such as (1) immortalization of antigen-specific human B cell hy... | 19,002,994 |
Effect of the water extracts of propolis on stimulation and inhibition of different cells. | The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts a... | 19,003,017 |
Isolation of progenitor cells from cord blood using adhesion matrices. | The aim of this study was to develop optimal conditions for selective adhesion and isolation of mesenchymal progenitor cells (MPCs) from cord blood and to determine their potential for osteogenic differentiation. Mononuclear cells (MNCs) were isolated by Ficoll-Paque gradient and plated onto 48-well culture plates prec... | 19,003,027 |
Analysis of Methylglyoxal Metabolism in CHO Cells Grown in Culture. | Recent evidence suggests that several unknown or ill-characterized factors strongly influence cell growth and function in culture. Isolating these factors is necessary in order to maximize culture productivities. Methylglyoxal (MG), a potent protein and nucleic acid modifying agent, has been identified as a player in t... | 19,003,028 |
Optimizing conditions for rat pancreatic islets isolation. | Many procedures have been described for rat pancreatic islet isolation. Several factors contribute to the pancreatic islet isolation outcome. One of the main problems in islet isolation procedure is the formation of a viscouse, gellike structure during collagenase digestion which entraps the free islets and decrease is... | 19,003,033 |
An Inexpensive Method for Applying Nitrogen Evaporation to Hexane-containing 24- or 96-well Plates. | A method is described for assembling an evaporation manifold from a cell culture flask, which allows for efficient nitrogen evaporation of hexane from 24- and 96-well plates. The precursor parts are readily available in most research laboratories. The nitrogen evaporation manifold is inexpensive, could possibly be used... | 19,003,065 |
Oral administration of milk fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 to DBA/1 mice inhibits secretion of proinflammatory cytokines. | We have previously shown that oral administration of skimmed milk(SM) fermented with Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 (OLL1073R-1/SM) to DBA/1 mice inhibited the development of collagen-induced arthritis (CIA). In this study, our aim was to examine possible mechanisms of inhibiting the development o... | 19,003,106 |
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