title stringlengths 0 1.13k | abstract stringlengths 1 15.7k | PMID int64 22 36.5M |
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De novo adipogenesis for reconstructive surgery. | Autografting of lost soft tissue is an important subject of the plastic and reconstructive surgery and autograft of fat pads has been only technique for this goal. However, the results are disappointing because of absorption of the grafts with time. Adipoblasts or adipocyte precursor cells distribute widely in connecti... | 19,003,121 |
Effects of green, oolong and black teas and related components on the proliferation and invasion of hepatoma cells in culture. | The effects of teas and related components on the proliferation and invasion of cancer cells were examined by employing both in vitro proliferation and invasion assay systems. Powdered green, oolong and black tea extracts dose-dependently inhibited proliferation and invasion of a rat ascites hepatoma cell line of AH109... | 19,003,122 |
Productivity enhancement of recombinant protein in CHO cells via specific promoter activation by oncogenes. | To construct a recombinant protein highly producing cell lines, we have previously developed the Oncogene Activated Production (OAP) system by using BHK-21 cells. Here we verified the availability of the OAP system in CHO cells. We firstly generated 'primed' ras amplified CHO cells, ras clone I, by introducing human c-... | 19,003,130 |
A serum-free culture system for efficient in vitro production of bovine blastocysts with improved viability after freezing and thawing. | The aim of this study was to evaluate whether two completely serum-free media (IVMD101 and IVD101) could improve the yield and quality of bovine blastocysts from in vitro matured and fertilized oocytes. The media were evaluated in the presence (IVMD101) or absence (IVD101) of bovine cumulus/granulosa cell (BCGC) cocult... | 19,003,132 |
Co-expression of bcl-2 and bag-1, apoptosis suppressing genes, prolonged viable culture period of hybridoma and enhanced antibody production. | Human bcl-2 and bag-1 DNA were introduced into mouse hybridoma 2E3- O cells and expressed. The expression of bcl-2 in BCMGneo-bcl2 transfectants was confirmed by ELISA and that of bag-1 in pZeo-bag1 was confirmed by western blotting. In batch cultures, the over-expression of bcl-2 prolonged the culture period by 2 days... | 19,003,134 |
The neurite-initiating effect of microbial extracellular glycolipids in PC12 cells. | The effects of several kinds of microbial extracellular glycolipids on neurite initiation in PC12 cells were examined. Addition of mannosylerythritol lipid-A (MEL-A), MEL-B, and sophorose lipid (SL) to PC12 cells caused significant neurite outgrowth. Other glycolipids, such as polyol lipid (PL), rhamnose lipid (RL), su... | 19,003,137 |
Modulation by extracellular matrices of monooxygenase and CYP1A1 induction in Hep G2 cells in serum-free culture. | The in vitro cellular functions of differentiated cells are influenced by culture conditions. Effects of several extracellular matrices (ECMs) on cytochrome P450-dependent monooxygenases (MFOs) induction and cytochrome P4501A1 (CYP1A1) gene expression were estimated in Hep G2 cells cultured in a serum-free medium. The ... | 19,003,142 |
Analysis of the use of fortified medium in continuous culture of mammalian cells. | Continuous culture is frequently used in the cultivation of mammalian cells for the manufacturing of recombinant protein pharmaceuticals. In such operations a large volume of medium is turned over each day, especially in the case where cell recycle, or perfusion cultivation, is practiced. In principle, the volumetric t... | 19,003,148 |
Development of an in vitro system for screening the ligands of a membrane glycoprotein CD36. | It has well been known that human and rodents exhibit a preference for fats. This suggests the existence of an orosensory system responsible for the detection of dietary fats. A plasma membrane glycoprotein CD36, besides the role in the uptake of long-chain fatty acids (LCFAs) as well as oxidized low-density lipoprotei... | 19,003,159 |
Cephalic hedgehog expression is regulated directly by Sox17 in endoderm development of Xenopus laevis. | In early development of animals, hedgehog (Hh) genes function as morphogen in the axis determination and the organ formation. In Xenopus, three hedgehog genes, sonic (shh), banded (bhh), and cephalic (chh), were identified and might organize various tissues and organs in embryogenesis. Here, we report the spatial and t... | 19,003,160 |
A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. | The Drosophila Schneider S2 (S2) Expression System enables expression of recombinant proteins constitutively, as well as inductively. This system can establish both transient and stable transformants with various selection markers. The generation of stable cell lines for increased expression or large scale expression o... | 19,003,171 |
Bioethics and biotechnology. | Biotechnology is at the intersection of science and ethics. Technological developments are shaped by an ethical vision, which in turn is shaped by available technology. Much in biotechnology can be celebrated for how it benefits humanity. But technology can have a darker side. Biotechnology can produce unanticipated co... | 19,003,197 |
Immuno-chromatographic assay for diagnosis of feline leukemia virus infection. | Feline leukemia virus (FeLV) infectious disease is one of feline infection diseases spreading broadly all over the world. For bedside diagnosis of FeLV infectious disease, an immuno-chromatographic assay was investigated. Five different monoclonal antibodies were developed against the major core protein FeLV-p27. Among... | 19,003,209 |
Development of monoclonal antibody against isoquinoline alkaloid coptisine and its application for the screening of medicinal plants. | In the development of immunoassay technique, the design of hapten containing a functional group suitable for protein conjugate is the key step for the preparation of antibodies against small molecules. Coptisine (MW 320), a bioactive constituent of Berberis and Coptis species, is small as an immunogen. In addition, cop... | 19,003,234 |
The use of hTERT-immortalized cells in tissue engineering. | The use of human telomerase reverse transcriptase (hTERT)-immortalized cells in tissue engineering protocols is a potentially important application of telomere biology. Several human cell types have been created that overexpress the hTERT gene with enhanced telomerase activity, extended life span and maintained or even... | 19,003,242 |
Microplates with integrated oxygen sensing for medium optimization in animal cell culture. | A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed me... | 19,003,253 |
Preparation of secretory vesicle-free plasma membranes by isopycnic sucrose gradient fractionation of neutrophils purified by the gelatin method. | Isolated human neutrophils serve as a model for the in vitro study of host defensive processes as well as the cell biology and biochemistry of primary human cells. We demonstrate that the requirements of the gelatinbased procedure for neutrophil isolation from whole blood induces the complete loss of secretory vesicles... | 19,003,266 |
Primary adipocyte culture: adipocyte purification methods may lead to a new understanding of adipose tissue growth and development. | In the present manuscript, the methods required to generate purified cultures of mature adipocytes, as well as stromal vascular cells, from the same isolation are detailed. Also, we describe the in vitro conditions for the dedifferentiation of the isolated mature adipocytes. These two types of cells may be used to reev... | 19,003,270 |
CB.Hep-1 hybridoma growth and antibody production using protein-free medium in a hollow fiber bioreactor. | The protein-free medium TurboDoma HP.1 (THP.1) was used to produce the CB.Hep-1 monoclonal antibody (mAb) in a CP-1000 hollow fiber bioreactor (HFB). This mAb is used for the immunopurification of recombinant hepatitis B surface antigen (rHBsAg), which is included in a vaccine preparation against the Hepatitis B Virus.... | 19,003,291 |
Epitope analysis of human monoclonal antibody specific for rice allergenic protein generated by in vitro immunization. | We previously established an in vitro immunization protocol for generating antigen specific human monoclonal antibodies (mAbs). In vitro immunization was performed against the soluble protein of rice allergenic protein (RA), resulting in the generation of three B cell clones, AC7-1/F9, CB7-1/E2 and CB7-8/F5, all of whi... | 19,003,321 |
Preparation of monoclonal antibody against crocin and its characterization. | Three crocin-carrier protein conjugates were synthesized and their hapten numbers were determined by matrix-assisted laser desorption/ionization time of flight mass spectrometry. Three monoclonal antibodies against crocin were produced by hybridomas fused with the splenocytes immunized with crocin hemisuccinate-bovine ... | 19,003,338 |
Hybridoma cell behaviour in continuous culture under hyperosmotic stress. | In this paper, we propose an alternative strategy to the ones proposed before (Oh et al., 1993; Øyaas et al., 1994a) to get real increases of global final antibody titer and production at hyperosmotic stress, by reducing the detrimental effect of such a stress on cell growth, and conserving the stimulating effect on an... | 19,003,339 |
Construction and characterization of stably transfected BHK-21 cells with human-type sialylation characteristic. | The human Golgi enzyme CMP-NeuAc:Gal(beta1-4)GlcNAc-R alpha2,6-sialyltransferase (ST6N) was stably coexpressed with human erythropoietin (EPO) from a BHK-21A cell line. The cell line was characterized with respect to the expression and in vitro activity of the ST6N and the endogenous alpha2,3-sialyltransferase. Detaile... | 19,003,352 |
Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma. | Apoptosis is an active, genetically determined death mechanism which can be induced by a wide range of physiological factors and by mild stress. It is the predominant form of cell death during the production of antibodies from murine hybridoma cell lines. A number of studies have now demonstrated that the suppression o... | 19,003,359 |
Cell engineering for muscle gene therapy: Extemporaneous production of retroviral vector packaging macrophages using defective herpes simplex virus type 1 vectors harbouring gag, pol, env genes. | Gene therapy as a treatment for neuromuscular diseases is an ever-developing concept based on the use of DNA as the therapeutic agent. In the search for appropriate strategies a bottleneck exists, however, concerning the targeting of vectors carrying the therapeutic gene, to all pathologic sites. These diseases are oft... | 19,003,367 |
Justification of continuous packed-bed reactor for retroviral vector production from amphotropic PsiCRIP murine producer cell. | To indentify a plausible large-scale production system forretroviral vector, three culture systems, i.e., batch culturewith medium exchange, microcarrier culture, and packed-bedreactor culture were compared. In batch cultures with mediumexchange, high cell concentrations were maintained for about amonth, and the harves... | 19,003,389 |
Isolation, characterization and recombinant protein expression in Veggie-CHO: A serum-free CHO host cell line. | The dihydrofolate reductase-deficient Chinese hamster ovary cell line, DXB11-CHO, commonly used as a host cell for the production of recombinant proteins requires 7.5% serum-supplementation for optimal growth. Regulatory issues surrounding the use of serum in clinical production processes and the direct and indirect co... | 19,003,404 |
Systems biology via redescription and ontologies (I): finding phase changes with applications to malaria temporal data. | Biological systems are complex and often composed of many subtly interacting components. Furthermore, such systems evolve through time and, as the underlying biology executes its genetic program, the relationships between components change and undergo dynamic reorganization. Characterizing these relationships precisely... | 19,003,444 |
Modelling molecular interaction pathways using a two-stage identification algorithm. | In systems biology, molecular interactions are typically modelled using white-box methods, usually based on mass action kinetics. Unfortunately, problems with dimensionality can arise when the number of molecular species in the system is very large, which makes the system modelling and behavior simulation extremely dif... | 19,003,449 |
Engineering-approach accelerates computational understanding of V1-V2 neural properties. | We present two computational models (i) long-range horizontal connections and the nonlinear effect in V1 and (ii) the filling-in process at the blind spot. Both models are obtained deductively from standard regularization theory to show that physiological evidence of V1 and V2 neural properties is essential for efficie... | 19,003,454 |
A retinal circuit model accounting for wide-field amacrine cells. | In previous experimental studies on the visual processing in vertebrates, higher-order visual functions such as the object segregation from background were found even in the retinal stage. Previously, the "linear-nonlinear" (LN) cascade models have been applied to the retinal circuit, and succeeded to describe the inpu... | 19,003,460 |
Outline of a novel architecture for cortical computation. | In this paper a novel architecture for cortical computation has been proposed. This architecture is composed of computing paths consisting of neurons and synapses. These paths have been decomposed into lateral, longitudinal and vertical components. Cortical computation has then been decomposed into lateral computation ... | 19,003,474 |
Quantitative characterization of animal behavior following blast exposure. | The simplest approach to quantifying animal behavior begins by identifying a list of discrete behaviors and observing the animal's behavior at regular intervals for a specified period of time. The behavioral distribution (the fraction of observations corresponding to each behavior) is then determined. This is an incomp... | 19,003,499 |
Dynamical aspects of behavior generation under constraints. | Dynamic adaptation is a key feature of brains helping to maintain the quality of their performance in the face of increasingly difficult constraints. How to achieve high-quality performance under demanding real-time conditions is an important question in the study of cognitive behaviors. Animals and humans are embedded... | 19,003,514 |
The neurodynamics underlying attentional control in set shifting tasks. | In this work we address key phenomena observed with classical set shifting tasks as the "Wisconsin Card Sorting Test" or the "Stroop" task: Different types of errors and increased response times reflecting decreased attention. A component of major importance in these tasks is referred to as the "attentional control" th... | 19,003,517 |
[Impact of motor and non-motor symptoms on the direct costs of Parkinson's disease]. | We evaluate the health-related economic burden of patients with Parkinson's disease (PD) and identify the impact of motor and nonmotor symptoms (NMS) and health-related quality of life (HRQL) on the direct costs of PD in Spain. A cross-sectional, multicenter, pilot study covering consecutive PD outpatients who were inc... | 19,003,550 |
[Impaired fasting glucose as a risk factor for atherothrombotic or lacunar cerebral infarction. A case-control study]. | Modifiable vascular risk factors (MVRF) for atherotrombotic or lacunar cerebral infarction have been clearly established, even though only a few population studies have been conducted and we do not know the risk of glycemic status prior to the diabetes mellitus (DM). This study has aimed to determine the relationship b... | 19,003,549 |
Improved synthesis of EM-1745, preparation of its C17-ketone analogue and comparison of their inhibitory potency on 17beta-hydroxysteroid dehydrogenase type 1. | Endocrine therapies are widely used for the treatment of estrogen-sensitive diseases. 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) is involved in the last step of the biosynthesis of potent estrogen estradiol (E(2)). This enzyme catalyzes the reduction of the C17-ketosteroid estrone (E(1)) into the C17beta-... | 19,003,559 |
Substrate specificity of thymidine phosphorylase of E. coli: role of hydroxyl groups. | Substrate specificity of E. coli thymidine phosphorylase to pyrimidine nucleoside modified at 5'-, 3'-, and 2'-positions of sugar moiety has been studied. Equilibrium (K(eq)) and kinetics constants of phosphorolysis reaction of nucleosides were measured. The most important hydrogen bonds in enzyme-substrate complex hav... | 19,003,566 |
Two new triterpenoid saponins from the flowers and buds of Lonicera japonica. | Two new triterpenoid saponins, loniceroside D (1) and loniceroside E (2), were isolated from the dry flowers and buds of Lonicera japonica Thunb., along with seven known compounds, chlorogenic acid (3), sweroside (4), vogeloside (5), epi-vogeloside (6), loniceroside A (7), loniceroside B (8), and loniceroside C (9). Th... | 19,003,609 |
Synthesis, structure, and bioevaluation of 2,5-bis(arylmethenyl)cyclopentanones. | Curcumin is an excellent lead compound with a variety of bioactivity. Recent articles reported that curcumin's instability and low bioavailability in vivo are mainly due to its easily decomposable beta-diketone moiety. With the aim of bioactive curcumin analogs with better pharmacokinetic property, we present here 11 b... | 19,003,615 |
Expression of the androgen receptor and syndecan-1 in breast tissue during different hormonal treatments in cynomolgus monkeys. | To analyze the expression of the androgen receptor(AR) and syndecan-1 in breast tissue during long-term hormonal treatment in cynomolgus monkeys. Sixty oophorectomized macaques were randomized to receive either tibolone, conjugated equine estrogens (CEE), CEE + medroxyprogesterone acetate (MPA) or no hormonal treatment... | 19,003,632 |
Serum inhibin A level in preeclampsia and normotensive pregnancy. | To compare serum inhibin A levels in pregnancy complicated by preeclampsia and in normotensive pregnancy. Blood samples were taken from 60 women. Thirty women were diagnosed with preeclampsia, and 30 women had normotensive pregnancies. Both groups were matched for gestational age. Blood samples were collected in plain ... | 19,003,635 |
Curable hypertension. | Hypertension due to a reninoma (suspected on the basis of biochemical and clinical features, and exquisite sensitivity of hypertension to angiotensin-converting enzyme [ACE] inhibition, but not visible on imaging) was cured by laparoscopic nephrectomy. Treatment was followed by an uneventful pregnancy. | 19,003,641 |
A broader view of justice. | In this paper I argue that a narrow view of justice dominates the bioethics literature. I urge a broader view. As bioethicists, we often conceive of justice using a medical model. This model focuses attention at a particular point in time, namely, when someone who is already sick seeks access to scarce or expensive ser... | 19,003,692 |
Serum carotenoid concentrations in postmenopausal women from the United States with and without osteoporosis. | Antioxidant defenses may be compromised in osteoporotic women. Little is known about fruit and vegetable or carotenoid consumption among postmenopausal women. The primary carotenoids in human serum are alpha- and beta-carotene, lycopene, beta-cryptoxanthin, lutein, and zeaxanthin. This study investigated the interrelat... | 19,003,732 |
Effects of yogurt supplemented with fish oil on plasma lipid and glucose concentrations, and liver lipid contents in mice. | The aim of this study is to clarify the effects of yogurt supplemented with fish oil on plasma lipid and glucose concentrations, and hepatic lipid contents in mice. Male Crlj:CD-1 (ICR) mice were fed five experimental diets for 12 weeks. The experimental diets were as follows: without yogurt and fish oil (control diet)... | 19,003,735 |
Incidence of venous thrombosis following peripheral arterial interventions. A prospective study. | The objective of the study was to investigate the incidence of deep vein thrombosis (DVT) at the puncture site following peripheral interventions and to assess if there is a difference between using a vascular closure by means of vascular closure systems or compression bandages. We prospectively included 474 consecutiv... | 19,003,747 |
[Analysis of nutritional status disorders in patients with chronic obstructive pulmonary disease]. | Among the most common extrapulmonary manifestations of COPD are nutritional status disorders. The specific loss of weight, called cachexia, characterized by loss of lean body mass in some COPD patients is observed. The aim of the study was the quantitative and qualitative analysis of COPD patients' nutritional status d... | 19,003,762 |
Ephrin-A5 regulates the formation of the ascending midbrain dopaminergic pathways. | Dopaminergic neurons from the substantia nigra and the ventral tegmental area of the midbrain project to the caudate/putamen and nucleus accumbens, respectively, establishing the mesostriatal and the mesolimbic pathways. However, the mechanisms underlying the development of these pathways are not well understood. In th... | 19,003,794 |
Improved desorption electrospray ionization mass spectrometry performance using edge sampling and a rotational sample stage. | The position of the surface to be analyzed relative to the sampling orifice or capillary into the mass spectrometer has been known to dramatically affect the observed signal levels in desorption electrospray ionization mass spectrometry (DESI-MS). In analyses of sample spots on planar surfaces, DESI-MS signal intensiti... | 19,003,852 |
Omics-based profiling of carcinoma of the breast and matched regional lymph node metastasis. | Axillary lymph node (ALN) status is currently used as an important clinical indicator of breast cancer prognosis. However, the molecular mechanisms underlying lymph node metastasis are poorly understood and the relationship between ALN metastasis and the primary tumor remains unclear. In an effort to reveal structural ... | 19,003,862 |
Identification of proteins that modify cataract of mouse eye lens. | The occurrence of a nuclear cataract in the eye lens due to disruption of the alpha3Cx46 connexin gene, Gja3, is dependent on strain background in a mouse model, implicating factors that modify the pathology. The differences upon cataractogenesis in the urea soluble proteins of the lens of two mouse strains, C57BL/6J a... | 19,003,866 |
A method based on bead flows for spot detection on 2-D gel images. | A method has been developed to detect spots on 2-D gel images. It is based on an analogy with beads flowing uphill on the surface of the gel image and on the analysis of their paths. This method does not need image smoothing, thereby allowing for conservation of the resolution of the original image. In addition, it all... | 19,003,871 |
Exhaled nitric oxide and respiratory symptoms in a community sample of school aged children. | To test the association between reported allergy and allergic diseases, respiratory symptoms, and the fractional concentration of exhaled nitric oxide (FeNO), in a community sample of school aged children. We administered a respiratory questionnaire and measured FeNO in a cross-sectional study of 1,135 children. FeNO w... | 19,003,883 |
Neuroscientific approaches and applications within anthropology. | Many of the most distinctive attributes of our species are a product of our brains. To understand the function, development, variability, and evolution of the human brain, we must engage with the field of neuroscience. Neuroscientific methods can be used to investigate research topics that are of special interest to an... | 19,003,891 |
Social organization of white-headed langurs (Trachypithecus leucocephalus) in the Nongguan Karst Hills, Guangxi, China. | The number of males per group is the most variable aspect of primate social organization and is often related to the monopolizability of females, which is mainly determined by the number of females per group and their reproductive synchrony. Colobines show both inter-specific and intra-specific variations in the number... | 19,003,910 |
Understanding hind limb weight support in chimpanzees with implications for the evolution of primate locomotion. | Most quadrupedal mammals support a larger amount of body weight on their forelimbs compared with their hind limbs during locomotion, whereas most primates support more of their body weight on their hind limbs. Increased hind limb weight support is generally interpreted as an adaptation that reduces stress on primates' ... | 19,003,921 |
Structural elucidation of four new furostanol saponins from Tupistra chinensis by 1D and 2D NMR spectroscopy. | Four new furostanol saponins (1-4), two pairs of diastereoisomers, were isolated from methanolic extracts of Tupistra chinensis rhizomes and their structures were assigned from (1)H and (13)C NMR spectra, DEPT, and by 2D COSY, NOESY, HMQC, and HMBC experiments. | 19,003,938 |
Immunological response to mistletoe (Viscum album L.) in cancer patients: a four-case series. | European mistletoe (Viscum album) has been used in complementary cancer treatment, but little is known concerning its effects on immunological parameters, although there is evidence that Viscum may stimulate the immune system. In this study, a trial was conducted with cancer patients to determine whether Viscum album e... | 19,003,944 |
Mesenchymal stem cell secretion of chemokines during differentiation into osteoblasts, and their potential role in mediating interactions with breast cancer cells. | Over 70% of patients with advanced breast cancer will develop bone metastases for which there is no cure. Mesenchymal Stem Cells (MSCs) and their derivative osteoblasts are subpopulations of cells within the bone marrow environment, postulated as potential interacting targets for disseminating cancer cells because of t... | 19,003,962 |
Rapid alterations in cortical protein profiles underlie spontaneous sleep and wake bouts. | Existing data indicate that sleep-wakefulness is an essential behavior. The biological function(s) of sleep, however, remains unknown, due, in part, to the lack of information available at the intracellular level. Preliminary microarray analyses show that changes in behavioral state influence regional mRNA profiles; ho... | 19,003,977 |
A penalized likelihood approach for mixture cure models. | Cure models have been developed to analyze failure time data with a cured fraction. For such data, standard survival models are usually not appropriate because they do not account for the possibility of cure. Mixture cure models assume that the studied population is a mixture of susceptible individuals, who may experie... | 19,003,981 |
Evaluation of molecular forms of prostate-specific antigen and human kallikrein 2 in predicting biochemical failure after radical prostatectomy. | Most pretreatment risk-assessment models to predict biochemical recurrence (BCR) after radical prostatectomy (RP) for prostate cancer rely on total prostate-specific antigen (PSA), clinical stage, and biopsy Gleason grade. We investigated whether free PSA (fPSA) and human glandular kallikrein-2 (hK2) would enhance the ... | 19,003,994 |
Crystal structure of a 1.6-hexanediol bound tetrameric form of Escherichia coli lac-repressor refined to 2.1 A resolution. | We report the structure of a novel tetrameric form of the lactose repressor (LacI) protein from Escherichia coli refined to 2.1 A resolution. The tetramer is bound to 1.6-hexanediol present in the crystallization solution and the final R(free) for the structure is 0.201. The structure confirms previously reported struc... | 19,004,002 |
Genetic and epigenetic inactivation of LPL gene in human prostate cancer. | Lipoprotein lipase (LPL) is in chromosome 8p22, site of one of the most common somatic deletions in prostate tumors. Additionally, a CpG island (CGI) was identified in the LPL promoter region. To test the hypothesis that LPL is a tumor suppressor gene, which is inactivated by somatic deletion and hypermethylation in pr... | 19,004,026 |
Predicting patient dissatisfaction following joint replacement surgery. | The incidence of patient-reported dissatisfaction following total joint arthroplasty can be up to 30%. Our aim was to identify the preoperative patient-level predictors of patient dissatisfaction 1 year after surgery. We surveyed 1720 patients undergoing primary hip or knee replacement surgery. Relevant covariates incl... | 19,004,032 |
Serum autoantibodies and their clinical associations in patients with childhood- and adult-onset linear scleroderma. A single-center study. | To determine the frequency of selected serum autoantibodies and their clinical associations in patients with childhood-onset (ChO) or adult-onset (AO) linear scleroderma (LiScl) evaluated at a single institution. Seventy-two patients (ChO = 40, AO = 32), including 12 with en coup de sabre, were studied. All ChO patient... | 19,004,036 |
An investigation of ADA compliance of aquatic facilities in the North Texas area. | The study expands research on accessibility, comparing compliance scores of aquatic facilities in North Texas built before the 1991 Title III Americans with Disabilities Act Accessibility Guidelines (ADAAG) with facilities built after the 1991 ADAAG and the proposed 2002 supplement. A quasi-experimental design directed... | 19,004,164 |
[Typical medical problems at the end of life: experiences in the intensive care unit]. | Current regulations regarding living wills need to be revised in order to avoid conflicts between patients or their relatives/surrogates on one side, and physicians on the other. For wording the will the patient should seek not only legal, but also medical advice. For doctors treating the patient, it would be helpful i... | 19,004,171 |
[Legal and practical aspects of euthanasia: experiences in The Netherlands]. | Following a debate of more than 30 years the "Dutch Termination of Life on Request and Assisted Suicide Act" has come into force in 2002. Basically, the termination of life on request will in accordance with the Criminal Code remain punishable. Punishability is precluded if the physician notifies the review committee t... | 19,004,180 |
Atherogenic lipoprotein subprofiling. | Elevated levels of low-density lipoprotein (LDL) cholesterol are a major risk factor for coronary heart disease. However, a significant number of coronary events occur in individuals with "normal" serum LDL cholesterol levels. It has been proposed that coronary heart disease (CHD) risk stratification may be improved by... | 19,004,193 |
Biomarkers related to aging in human populations. | Biomarkers are increasingly employed in empirical studies of human populations to understand physiological processes that change with age, diseases whose onset appears linked to age, and the aging process itself. In this chapter, we describe some of the most commonly used biomarkers in population aging research, includ... | 19,004,190 |
Effects of the drug (BSZGC)-containing serum on proliferation of rat's osteoclasts and TRACP activity in vitro. | To observe effects of the drug-containing serum of Bu Shen Zhuang Gu Capsule (BSZGC Capsule for Tonifying the Kidney to Strengthen the Bones) on proliferation of the rat's osteoclasts and tartrate-resistant acid phosphatase (TRACP) activity in vitro so as to delve into the mechanisms of its preventive and therapeutic a... | 19,004,206 |
[Haemorrhagic complications among patients on life-long acenocumarol therapy]. | The aim of our study was to determine factors influencing occurrence of haemorrhagic events during acenocumarol therapy. A total of one hundred and forty patients were interviewed using a questionnaire. From one hundred forty (100%) of interviewed patients, forty one (29.3%) had haemorrhagic events and in eighteen (12.... | 19,004,232 |
Characterisation of actinomycetes isolated from ancient stone and their potential for deterioration. | Actinomycetes have been isolated from decayed and sound stone samples taken from a tomb site at Tell Basta, Zagazig City, Egypt. A total of 160 isolates have been characterised. The numbers and distribution of actinomycetes were studied during different seasons; during the winter months (18-20 degrees C), actinomycete ... | 19,004,242 |
Evaluation of antibacterial activity of synthetic aliphatic and aromatic monoacylglycerols. | The antibacterial activity of synthetic aliphatic and aromatic monoacylglycerols (MAGs) was studied against two human pathogens: Staphylococcus aureus and Escherichia coli. The active compounds inhibited selectively S. aureus. The most active compounds amongst them were those with medium size aliphatic chain and aromat... | 19,004,249 |
[Selected applications of diode laser in laryngological surgery]. | Diode laser due to emitted wavelength and achieved tissue effect appear to be universal tool for broad range of applications in laryngological surgery. The aim of the study was to present variety of applications of this type of laser in the treatment of selected pathologies of the nose, pharynx and oral cavity. 45 pati... | 19,004,255 |
[Reconstruction of the hypopharynx and cervical esophagus with gastric pull-up technique in the patient with recurrence carcinoma of the hypopharynx]. | Patient with hypopharyngeal cancer are difficult to treat because they usually present with advanced disease, poor general health status and severe nutritional problems. Because of the poor prognosis, careful consideration has to be given regarding the choice of the correct surgical approach for respirato-digestive tra... | 19,004,269 |
[CMV-induced cell death and fas gene expression in resting and proliferating human fibroblasts]. | Cytomegalovirus (CMV) infection development and mRNA fas transcription levels (CD95) in resting (GO) and proliferating (S-phase) human lung embryo fibroblasts (HLEF-110044 line) were studied. In GO cells accumulation of infectious CMV was high and cell death was very quick, and fas gene expression was inhibited in earl... | 19,004,284 |
[Influence of antisense RNA and sequences of viral transactivators traps on RNA synthesis of HTLV-1 virus]. | Significant number of scientific publications devoted to inhibition of viral replication by antisense RNA (asRNA) genes shows that this approach is useful for gene therapy of viral infections. To investigate the possibility of suppression of HTLV-1 virus reproduction by asRNA we constructed recombinant plasmids contain... | 19,004,285 |
[Genetic identification as method of detection of pathogenic and symbiotic strains of enterococci]. | Comparative study of 97 cultures of enterococci, including reference highly virulent strain Enterococcus faecalis ATCC 259212, 35 clinical isolates of E. faecium, 58 isolates of E. faecalis, and 3 probiotic strains of E. faecium: Linex (Lek company, Slovenia), SF68 (Bifiform, Denmark), and L3 (Avena, Russia). Thirteen ... | 19,004,287 |
Unusual evolution of a residual dental cyst: a giant rhinolith. | Rhinoliths are calcareous concretions, consisting mainly of calcium and magnesium carbonates and phosphates. They can vary in size and shape, from small bodies to massive growths, and may invade adjacent structures. A 75-year-old female with a history of purulent discharge, nasal obstruction, and headache was observed ... | 19,004,311 |
[Comparison of effects of systemic (intramuscular) and intrastriatal injections of D1-dopamine receptor blocker on motor behavior and postural adjustment in dogs]. | The study is based on the concept (Shapovalova, 2000) that activation and blockade of various types of muscarine and dopamine receptors located at various outputs of neostriatum should have different effects on motor behavior. The goal of the study was comparison of effects of systemic and intrastriatal injections of a... | 19,004,318 |
[Molecular-genetic and epidemiologic examination of personnel subjected to occupational irradiation in the Russian Federal Nuclear Center (Sarov). Report 2. Genetic and non-genetic risk factors of morbidity]. | Below is given a comparative evaluation of the genetic and the epidemiological examination of the cohort comprised by VNIIEF personnel subjected chronically to gamma-neutron ionizing radiation (74 persons), as well as nonirradiated cohort (74 persons). There are obtained data on the influence of the integral genotype s... | 19,004,325 |
[Thiomorpholine transformation by the fungus Bjerkandera adusta]. | A screening of lignin-degrading basidial fungi that can grow in the presence of thiomorpholine derivatives (the mixture of 1,4-perhydrothiazines) has been performed. Strain Bjerkandera adusta VKM F-3477 was shown to have the maximal rate of growth in the presence of these compounds, and its capacity for thiomorpholine ... | 19,004,342 |
The right to access health care: health care according to European social security law instruments. | In this contribution we will look at how the traditional European social security conventions shape the fundamental right to health care. As to the instruments under investigation we focus upon the regional agreements that have been enacted within the framework of the Council of Europe. More specifically we will discus... | 19,004,379 |
Claiming access to health care in The Netherlands under international treaty law. | In the Netherlands, access to healthcare has been guaranteed by social health insurance legislation. But since the introduction of the health insurance system in the 1960s, it has been under frequent review, the latest reform was the introduction of regulated competition. At the same time, claiming health insurance ent... | 19,004,383 |
Restaurant inspection frequency and food safety compliance. | Although food premises are regularly inspected, little information is available on the effect of inspections on compliance records, particularly with respect to the impact of the frequency of inspection on compliance. The following presents the outcome of a study designed to assess the impact of increased inspection fr... | 19,004,396 |
A randomized prospective trial of the cutaneous and sensory effects of feminine hygiene wet wipes. | To assess the effects of feminine hygiene wet wipes on vulvar skin. An examiner-blind, randomized, prospective clinical trial of prototype wet wipes (treatment group) and dry toilet tissue (comparison group) in 120 premenopausal and 60 postmenopausal women was conducted in which the wipes or toilet tissues were for app... | 19,004,402 |
Subgaleal hemorrhage after the use of a vacuum extractor during elective cesarean delivery: a case report. | Subgaleal hemorrhage (SGH) is a rare but possibly fatal condition in the neonate. Vacuum extractors are effective options for operative vaginal delivery. However, their use can increase the risk of intracranial hemorrhage in the neonate. A male infant delivered by cesarean section with assistance of a vacuum extractor ... | 19,004,410 |
MDMA-assisted psychotherapy using low doses in a small sample of women with chronic posttraumatic stress disorder. | The purpose of this study was to investigate the safety of different doses of MDMA-assisted psychotherapy administered in a psychotherapeutic setting to women with chronic PTSD secondary to a sexual assault, and also to obtain preliminary data regarding efficacy. Although this study was originally planned to include 29... | 19,004,414 |
Availability and characteristics of betel products in the U.S. | Betel use involves oral placement of shards of areca nut (Areca catechu palm seed containing the cholinergic agonist arecoline) wrapped with slaked lime in a betel leaf (Piper betle plant) or various chopped or powdered products containing areca nut and/or parts of the betel plant. Additives to this mixture include cat... | 19,004,423 |
On-line flow injection-cloud point preconcentration of polycyclic aromatic hydrocarbons coupled with high-performance liquid chromatography. | Cloud point methodology has been used to develop a novel preconcentration and an analytical method for polycyclic aromatic hydrocarbons (PAHs) in soil sample. The nonionic surfactant Tergitol 15-S-7 was successfully used as the surfactant-mediated extractant in both ultrasonic and microwave-assisted extractions. Over 9... | 19,004,448 |
Geochemical characteristics and fluxes of organic carbon in a human-disturbed mountainous river (the Luodingjiang River) of the Zhujiang (Pearl River), China. | This study aims to investigate the state of the riverine organic carbon in the Luodingjiang River under human impacts, such as reforestation, construction of reservoirs and in-stream damming. Seasonal and spatial characteristics of total suspended sediment (TSS), dissolved organic carbon (DOC) and particulate organic c... | 19,004,473 |
Determination of phenolic endocrine disrupting chemicals and acidic pharmaceuticals in surface water of the Pearl Rivers in South China by gas chromatography-negative chemical ionization-mass spectrometry. | An analytical method for phenolic endocrine disrupting chemicals and acidic pharmaceuticals in river water was developed using gas chromatography mass spectrometry (GC-MS) coupled with negative chemical ionization (NCI) technique, and used for the determination of these compounds in the Pearl Rivers (Liuxi, Zhujiang an... | 19,004,474 |
Occurrence, properties and pollution potential of environmental minerals in acid mine drainage. | This paper describes the occurrences, the mineralogical assemblages and the environmental relevance of the AMD-precipitates from the abandoned mine of Valdarcas, Northern Portugal. At this mining site, these precipitates are particularly related with the chemical speciation of iron, which is in according to the abundan... | 19,004,477 |
Estrogen receptor modulators and estrogen receptor beta immunolabelling in human umbilical vein endothelial cells. | Human umbilical vein endothelial cells (HUVEC) exposed to the female sex hormone estradiol show different kinds of effects including increased elasticity, activation of plasma membrane Na+/H+ exchange, prostacyclin production, prevention of apoptosis and many others. The aim of this study was the systematic analysis of... | 19,004,482 |
A new locus for congenital cataract, microcornea, microphthalmia, and atypical iris coloboma maps to chromosome 2. | To report a novel phenotype of autosomal dominant atypical congenital cataract associated with variable expression of microcornea, microphthalmia, and iris coloboma linked to chromosome 2. Molecular analysis of this phenotype may improve our understanding of anterior segment development. Observational case study, genom... | 19,004,499 |
Self-medication of mood disorders with alcohol and drugs in the National Epidemiologic Survey on Alcohol and Related Conditions. | Using alcohol or drugs to reduce emotional distress (self-medication) has been proposed as an explanation for the high comorbidity rates between anxiety and substance use disorders. Self-medication has been minimally studied in mood disorders despite equally high rates of alcohol and drug use. Data came from the Nation... | 19,004,504 |
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