title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
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Possible role of cyclic nucleotides in the mechanism of the protective effect of methylprednisolone on the hypoxic rat heart. | The isolated isovolumic rat heart was used as a model of cardiac hypoxia. Force of cardiac contraction and cardiac cyclic nucleotide levels (cyclic GMP and cyclic AMP) were monitored in hearts subjected to hypoxia for 5 min and allowed to recover by reoxygenation. Hearts were obtained from both control animals and anim... | 214,463 | [
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Inhibition of plasminogen activator secretion by cyclic AMP in a macrophage-like cell line. | The continuous cell line, J774.2, exhibits many macrophage-like functions such as latex and Fc-mediated phagocytosis, antibody mediated phagocytosis, antibody mediated cytotoxicity, chemotaxis, and lysozyme secretion. Cyclic AMP stimulates Fc-mediated phagocytosis and inhibits the growth of J774.2. To further evaluate ... | 214,471 | [
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Characterization of a specific adenosine receptor on human lymphocytes. | We have examined the mechanism of action of adenosine, a naturally occurring nucleoside that has profound effects on lymphocyte function. Adenosine (0.01 micrometer to 10 micrometer) increased lymphocytes cAMP levels in a dose-dependent fashion with a maximal (10 micrometer) increase of about 4-fold, whereas adenine, g... | 214,496 | [
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Comparative toxicity of polychlorinated biphenyl and polybrominated biphenyl in the rat liver: light and electron microscopic alterations after subacute dietary exposure. | The comparative toxicity of polychlorinated biphenyl (PCB) and polybrominated biphenyl (PBB) in livers was studied in male Holtzman rats. Four-week-old animals were fed at dietary levels of 0, 5, 50, or 500 ppm for 5 weeks and then sacrificed. The mean liver-body weight ratios of the 50 and 500 ppm groups were increase... | 214,504 | [
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Preparation of functional smooth muscle cells from the rabbit aorta. | A procedure for dissociating the rabbit aorta into single, functional smooth muscle cells is described. After removal of adventitia and intima, slices of media were incubated with purified collagenase, elastase, and soybean trypsin inhibitor in a Krebs-Ringer buffer modified with Hepes, amino acids, and a [Ca2+] of 0.2... | 214,509 | [
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Specific adsorption of H-2-restricted cytotoxi T cells to macrophage monolayers. | These experiments tested whether Tc cells specific for foreign antigen (X) plus self H-2 adsorbed to macrophage monolayers displaying (a) X with allogeneic H-2; (B) self H-2 alone; (c) X plus self H-2. Specific adsorption occurred only in case (c), a result compatible with altered self and requiring further operational... | 214,513 | [
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The specificity of pyrophosphate myocardial scintigrams in patients with prior myocardial infarction: concise communication. | Fifty-five patients with old (9 days to 10 yr) transmural infarcts but with no evidence of recent infarction, were imaged with Tc-99m pyrophosphate. Discrete uptake was rare in the setting of an old infarct. Diffuse uptake was neither sensitive to, nor specific for, acute infarction. Prior infarction will rarely cause ... | 214,527 | [
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Dopamine receptors in the central thermoregulatory pathways of the rat. | 1. Intrahypothalamic injection of either dopamine (10 microgram) or apomorphine (10 microgram) in a dose volume of 1 microliter. caused an almost immediate rise in tail skin temperature and a concomitant fall in core temperature in the conscious rat maintained at an ambient temperature of 17 +/- 1 degrees C. 2. The loc... | 214,547 | [
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Potential bis-alkylating agents for cancer chemotherapy. Approaches to the synthesis of 2-sulfonyl-1,4-bis(methanesulfonoxy)butanes. | Three general methods for the synthesis of derivatives of the bis-alkylating agent, myleran [1,4-bis(methane-sulfonoxy)butane (1)], are presented. These derivatives contain a lipophilic group attached to the 2 position of 1,4-bis(methanesulfonoxy)butane by means of a sulfonyl function. As examples of the scope of the m... | 214,556 | [
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Jejunal microbial flora of southern indian infants in health and with acute gastroenteritis. | The microbial flora of the jejunal lumen of 28 infants with acute gastroenteritis was compared with that of a group of 10 normal infants. The jejunum of control subjects harboured an "oral" type of flora and in a few instances enterobacteria in small numbers. The concentrations of all but one of the groups of organism ... | 214,565 | [
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DNA of Epstein-Barr virus. IV. Linkage map of restriction enzyme fragments of the B95-8 and W91 strains of Epstein-Barr Virus. | The arrangement of EcoRI, Hsu I, and Sal I restriction enzyme sites in the DNA of the B95-8 and W91 isolates of Epstein-Barr virus (EBV) has been determined from the size of the single-enzyme-cleaved fragments and from blot hybridizations that identify which fragments cut from the DNA with one enzyme contain nucleotide... | 214,576 | [
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Absence of circularly permuted and largely redundant sequences in the genome of visna virus. | A previous study of the infectivity of visna virus proviral DNA suggested that the genetic information of the virus is distributed over at least two of the RNA subunits. Because the genetic complexity of visna virus corresponds to the size of one subunit, this result may imply that sequence redundancies exist within ea... | 214,577 | [
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Modulation of mouse mammary tumor virus production in the MJY-alpha cell line. | Implantation of the mouse mammary tumor virus (MMTV)-producing mammary tumor cell line MJY-alpha into isogeneic mice elicited both humoral and T-cell responses against MMTV virion antigens. The carcinosarcomas which developed from the implanted cells showed a significant decrease in MMTV synthesis, compared with cells ... | 214,582 | [
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Chromatin pattern consisting of repeating bipartite structures in WI-38 cells infected with human cytomegalovirus. | A novel chromatin structural pattern displaying bipartite and oblate ellipsoid structures orderly arranged along a fiber axis has been observed in WI-38 cells infected with human cytomegalovirus. This chromatin type coexists with chromatin fibers showing conventional nucleosomes. Each bipartite-oblate structure is 40 n... | 214,585 | [
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[The judgement of collagen metabolism by hydroxyproline in plasma and urine and plasma collagenic peptidase under different diet (author's transl)]. | In 34 subjects--coal workers with pneumoconiosis, patients with collagenic diseases and healthy subjects--the daily urinary excretion of hydroxyproline (HP) and the plasma concentrations of HP and of collagenic peptidase have been measured during one week under HP-free and HP-enriched diet. It is shown that a 24-hour p... | 214,624 | [
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Inhibition of the binding of low-density lipoprotein to its cell surface receptor in human fibroblasts by positively charged proteins. | A group of proteins and polyamino acids with positively charged domains were shown to inhibit the binding of 125I-LDL to its receptor on the surface of human fibroblasts. The list of inhibitory proteins included platelet factor 4 (which has a cluster of lysine residues at its carboxyl terminus), two lysine-rich histone... | 214,639 | [
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[Sudden deafness and cytomegalievirusinfection of adults (author's transl)]. | By means of virologuc--serologic examination methods it was possible to find in four patients with sudden deafness a cytomegaloinfection, whereby the complement-fixation reaction was determined with the CMV antigen. With the usual therapeutical measures all cases with an, in part high-degree, labyrinthine deafness impr... | 214,643 | [
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Electron microscopic studies of tumor viruses. II. Entry and uncoating of Epstein-Barr virus. | Entry of Epstein-Barr virus into human lymphoblastoid cells (Daudi cells) was studied by electron microscopy. At the site of viral attachment, two distinct interactions conducive to penetration of the virus occurred between the viral envelope and cell membrane, namely, (i) simultaneous dissolution of both the envelope ... | 214,669 | [
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Epstein-Barr virus early antigen titer by immunofluorescence in microplates. A new semi-automated method based on microtiter system. | For the titration of antibodies directed against Epstein-Barr Virus early antigen (EBV-EA) we describe a method in microplates with the Microtiter System (Cooke Engineering Co., Alexandria, Va). Using this technique, which allows rapid epidemiological investigations, we have titered anti-EBV-EA antibodies in different ... | 214,682 | [
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Perturbations in simian virus 40 DNA synthesis by ultraviolet light. | Perturbations of Simian Virus 40 (SV40) DNA replication by ultraviolet (UV) light during the lytic cycle in permissive monkey CV-1 cells resemble those seen in host cell DNA replication. Formation of Form I DNA molecules (i.e. completion of SV40 DNA synthesis) was more sensitive to UV irradiation than synthesis of repl... | 214,706 | [
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The role of interferon in virus infections and antibody formation. | Virus-type interferon is capable of suppressing the antibody response both in vivo and in vitro. Interferon induced by antigen or mitogen (immune interferon), primarily in T-lymphocytes, also appears capable of suppressing the antibody response. Cyclic AMP has a regulatory effect on the induction of both virus-type and... | 214,736 | [
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Formation of protein micelles from amphiphilic membrane proteins. | The membrane penicillinase (penicillin amido-beta-lactamhydrolase, EC 3.5.2.6) from Bacillus licheniformis, the Semliki Forest virus spike proteins, and the Sendai virus glycoproteins have each been isolated as soluble protein aggregates that are virtually free of lipid and detergent. The sedimentation coefficients of ... | 214,782 | [
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Quantitative determination of the lateral diffusion coefficients of the hormone-receptor complexes of insulin and epidermal growth factor on the plasma membrane of cultured fibroblasts. | Fluorescent derivatives of insulin and epidermal growth factor bound to 3T3 mouse fibroblasts are mobile on the cell surface, with similar diffusion coefficients, D approximately (3--5) x 10(-10) cm2/sec at 23 degrees C. Increasing the temperature to 37 degrees C results in rapid receptor immobilization. The immobiliza... | 214,784 | [
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Prostacyclin: a potent stimulator of adrenal steroidogenesis. | The relative potencies of various prostaglandins were investigated in trypsin-dispersed cat adrenocortical cells. Prostacyclin proved to be the most potent steroidogenic prostaglandin, being 100-1000 times more potent than PGE2. This stimulant effect of prostacyclin was only partially dependent upon the presence of ext... | 214,824 | [
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Octopamine receptors, adenosine 3',5'-monophosphate, and neural control of firefly flashing. | An adenylate cyclase activated as much as 25-fold by low concentrations of octopamine has been identified in the firefly lantern. The relative potency of octopamine and various other amines in stimulating this enzyme, and effects of antagonists in blocking octopamine activation, correlate well with the known effects of... | 214,856 | [
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[Multiple myeloma and acute leukemia. Simultaneous evolution of plasmacyte and myelomonocytic clones]. | The authors report two cases of acute myelo-monocytic leukemia occuring during the course of multiple myeloma treated by local radiotherapy and melphalan. Both patients underwent a complete remission of their myeloma for 27 and 78 months. The myeloma relapsed suddenly in the form of an acute leukemia. In one case, the ... | 214,859 | [
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[A new treatment of Huntington's chorea. Report of three cases (author's transl)]. | The authors report their experience in three cases of Huntington's chorea treated with tiapride (Tiapridal). A real efficacy and an interesting effect of the drug were observed on choreic hyperkinesias. No regression of the other symptoms were noted and the course of the disease not changed. | 214,869 | [
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[Immune response of pigs to Aujeszky disease virus and swine influenza virus]. | Explored was the possibility of simultaneous vaccination of pigs against the Aujeszky's disease virus and the swine influenza virus. Used were strain MK-25 against the former and strain 3sb against the latter. It was found that at the simultaneous subcutaneous or oral treatment with the two antigens equally effective i... | 214,936 | [
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... |
Tumor cell interactions in vitro microtubules, 100 angstrom filaments, and contractile microfilaments of tumor cells involved in "emperipolesis". | An increase in the number of examples of emperiopolesis, i.e., cells within cells, was observed subsequently to addition of Bt2cAMP to spinner flask cultures of JB-1-E tumor cells. A conspicuous arrangement of microtubules and 100 angstrom filaments in the tumor cells involved in emperiopelesis was observed. Absence of... | 214,966 | [
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... |
Effect of ouabain and colloid osmotic pressure on renal tubule cell volume. | Proximal renal tubule cell volume increases in ouabain but cell swelling is limited by the tubule basement membrane (TBM) and the colloid osmotic pressure from the bath protein. We compared the effect of ouabain, external protein concentration, and TBM on cell volume of proximal convoluted (PCT), proximal straight (PST... | 215,039 | [
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Acute toxicity of hydrogen cyanide to freshwater fishes. | Acute toxicity of hydrogen cyanide was determined at various temperatures from 4 degrees to 30 degrees C and oxygen concentrations of 3.36 to 9.26 mg/L on different life history stages of five species of fish: fathead minnow, Pimephales promelas Refinesque; bluegill, Lepomis macrochirus Rafinesque; yellow perch, Perca ... | 215,091 | [
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Microanalytical RPC-5 columns for rapid chromatography of aminoacyl-tRNAs. | A microanalytical reversed-phase chromatographic system for the separation of aminoacyl-tRNA's on microbore (0.3cm X 25cm) columns is described. Chromatography of lysyl-tRNA from polyoma virus transformed mouse fibroblasts revealed at least seven identifiable isoacceptors. The system has the advantages of speed, sensit... | 215,093 | [
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... |
Effects of a dopamine agonist piribedil in depressed patients: relationship of pretreatment homovanillic acid to antidepressant response. | Piribedil, a compound that stimulates dopamine receptors in a relatively specific fashion, was administered to 11 hospitalized depressed patients. The dopamine agonist significantly decreased rapid eye movement (REM) sleep and percent REM sleep and increased REM latency. Piribedil decreased the probenecid-induced accum... | 215,097 | [
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Iron and the liver: subcellular distribution of iron and decreased microsomal cytochrome P-450 in livers of iron-loaded rats. | To understand better the intracellular iron distribution and metabolic consequences of chronic hepatic iron overload, rats were given large doses of iron dextran or ferric citrate intraperitoneally. They accumulated large quantities of iron within Kupffer cells and hepatocytes. The relative subcellular iron distributio... | 215,100 | [
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Permeability changes of plasma and lysosomal membranes in HeLa cells infected with rabbit poxvirus. | Infection of HeLa-cell monolayer cultures with rabbit poxvirus induces a marked decrease in cell-associated protein and in the activities of lactate dehydrogenase, acid phosphatase, and beta-glucuronidase. This effect begins to occur around 10 hours post-infection (p.i.) and is accompanied by a concomitant rise of thes... | 215,105 | [
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The interaction between mitochondrial NADH-ubiquinone oxidoreductase and ubiquinol-cytochrome c oxidoreductase. Evidence for stoicheiometric association. | 1. The NADH-ubiquinone oxidoreductase complex (Complex I) and the ubiquinol-cytochrome c oxidoreductase complex (Complex III) combine in a 1:1 molar ratio to give NADH-cytochrome c oxidoreductase (Complex I-Complex III). 2. Experiments on the inhibition of the NADH-cytochrome c oxidoreductase activity of mixtures of Co... | 215,122 | [
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Effect of intessive plasmapheresis on the plasma cholesterol concentration with familial hypercholesterolemia. | Plasmapheresis was studied as a means of reducing the serum cholesterol concentration in 3 hypercholesterolemic patients who each underwent courses of intensive plasmapheresis with removal of 250--500 ml of plasma each day for 5--9 days. In one homozygous Type II patient, the serum cholesterol concentration decreased f... | 215,170 | [
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Potentiation of isoprenaline-induced plasma cyclic AMP response by aminophylline in normal and asthmatic subjects. | 1 The effect of intravenous aminophylline infusion (6 mg-1 kg in 20 min, then 0.9 mg-1 kg-1 h thereafter) and its interaction with inhaled isoprenaline (800 microgram ex inhaler) on plasma cyclic AMP levels was studied in five normal and five asthmatic subjects. 2 Aminophylline infusion alone did not significantly chan... | 215,179 | [
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0.19669... |
Combined coenzyme-substrate analogues of various dehydrogenases. Synthesis of (3S)- and (3R)-5-(3-carboxy-3-hydroxypropyl)nicotinamide adenine dinucleotide and their interaction with (S)- and (R)-lactate-specific dehydrogenases. | Two diastereomeric nicotinamide adenine dinucleotide (NAD+) derivatives were synthesized in which the substrates of (S)-and (R)-lactate-specific dehydrogenases are covalently attached via a methylene spacer at position 5 of the nicotinamide ring. The corresponding nicotinamide derivatives were obtained stereospecifical... | 215,189 | [
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Generation of a free alpha-amino group by Raney nickel after 2-nitro-5-thiocyanobenzoic acid cleavage at cysteine residues: application to automated sequencing. | The selective reaction of SH containing proteins and peptides with NTCB (2-nitro-5-thiocyanobenzoic acid) has been reported (Degani, Y., & Patchornick, A. (1974) Biochemistry 13, 1; Jacobson, G.A., Schaffer, M.H., Stark, G.R., & Vanaman, T.C. (1973) J. Biol. Chem. 248, 6583). With this reagent, cysteinyl peptid... | 215,200 | [
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... |
Activation of mammalian cyclic AMP phosphodiesterases by trypsin. | BHK fibroblasts contain two forms of cyclic AMP phosphodiesterase 3':5'-cyclic nucleotide 5'-nucleotidohydrolase EC 3.1.4.17) as analyzed by linear sucrose gradient fractionation; a 3.6-S form (peak I) and a 6.7-S form (peak II). Peak I is specific for cyclic AMP as substrate and displays Michaelis-Menten kinetics with... | 215,213 | [
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Isolation and partial characterization of secreted mouse pituitary prolactin. | Prolactin secreted by mouse anterior pituitaries was purified by gel filtration on Sephadex G-100. Electrophoretic homogeneity of the purified hormone was demonstrated in several gel systems, and electrophoresis in the presence of sodium dodecyl sulfate indicated an apparent molecular weight of 21 000 +/- 2000. Mouse a... | 215,218 | [
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Modulation of platelet responsiveness through selective phosphorylation of plasma membrane proteins. | 32P phosphorylation of plasma membranes from human blood platelets, under conditions that closely resemble physiological ones (endogeneous phosphate donors and intact platelets in homologous plasma), result in the incorporation of the label mainly in a membrane glycoprotein of apparently high molecular weight (greater ... | 215,226 | [
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Kinetic and pharmacologic characterization of gamma-aminobutyric acid receptive sites from mammalian brain. | Sodium-dependent (+Na) and sodium-independent (-Na) receptive sites for gamma-aminobutyric acid (GABA) have been characterized using synaptic plasma membranes from bovine and rat brain. Synaptic plasma membranes were prepared from either rat cerebellar cortex or calf cerebral cortex by discontinuous sucrose gradient fl... | 215,278 | [
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0.2703073,
... |
Lung cancer as a second primary. | An unusually high association of other primary cancers (9.7%) was found during the analysis of 403 consecutive cases of carcinoma of the lung diagnosed at DGMC between 1960 and 1975. Incidence by stage included 17.3% for Stage I (75 cases) and 16.9% for Stage II (59 cases). Median survival by stage was not adversely af... | 215,300 | [
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... |
Experiences with thymosin in primary immunodeficiency disease. | Thymosin has no effect in vitro on cyclic AMP levels in thymocytes. However, when thymosin was injected into patients lacking "serum factor" (SF) activity, it induced the appearance of SF or SF-like activity and the disappearance of target cells for SF among the peripheral blood lymphocytes of the treated patients. On ... | 215,306 | [
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0.04630772,
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Transformation of hamster embryo fibroblasts by a specific fragment of the herpes simplex virus genome. | Isolated restriction endonuclease fragments of the herpes simplex virus type 1 (HSV-1) genome were introduced into hamster embryo cells to identify DNA sequences capable to transforming the cells with respect to acquisition of properties correlated with tumorigenicity. One of the fragments generated by cleavage of HSV-... | 215,318 | [
0.04799893,
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Metabolism of acetylcholine receptor in chick embryo muscle cells: effects of RSV and PMA. | We have investigated some aspects of the metabolism of the integral membrane protein acetylcholine receptor (AChR) in normal and transformed cultures of chick embryo muscle cells. Turnover of AChR in control muscle cell cultures was compared with turnover in cultures infected and transformed by a temperature-sensitive ... | 215,321 | [
0.2148419,
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0.3522618,
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Plasminogen activator in chick embryo muscle cells: induction of enzyme by RSV, PMA and retinoic acid. | To explore the generality of the effects of sarcoma viruses, tumor-promoting phorbol esters and retinoic acid, we have studied plasminogen activator production in differentiating chick myogenic cultures. Although slightly higher than in chick fibroblast cultures, the level of spontaneously synthesized enzyme is low; it... | 215,322 | [
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0.5992757,
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... |
Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. | Three major species of viral DNA have been observed in cells infected by retroviruses: a linear, double-stranded copy of a subunit of viral RNA; closed circular DNA; and proviral DNA inserted covalently into the genome of the host cell. We have studied the structures of the unintegrated forms of avian sarcoma virus (AS... | 215,324 | [
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... |
The matrix (M) protein of vesicular stomatitis virus regulates transcription. | Temperature-sensitive mutants of vesicular stomatitis virus (VSV) belonging to complementation group III contain a lesion in the matrix (M) protein. This results in a 2--5 fold increase in transcription at the nonpermissive temperature. Co-infection of cells with one of these mutants and wild-type virus reverses this m... | 215,330 | [
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0.2649021,
0.5261446,
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Rapid fluorometry of estrogens in nonpregnancy urine, with use of chloroform extraction and purification by anion-exchange chromatography. | We describe a new method of extraction and purification of estrogens in low concentration in urine, involving a rapid enzymic hydrolysis of a 10-mL sample, automatic extraction (in a tube) with CHCl3/ethyl acetate, purification by chromatography on a disposable "mini-column" of AG 1-X2, and fluorometry by continuous fl... | 215,343 | [
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0.2041529,
0.1336685,
0.01809197,
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Lactate-to-pyruvate or pyruvate-to-lactate assay for lactate dehydrogenase: a re-examination. | The pyruvate-to-lactate assay for determining lactate dehydrogenase (EC 1.1.1.27) can now yield linearity equal to or better than that obtained by the lactate-to-pyruvate assay. In addition, there are significant advantages to the pyruvate-to-lactate reaction: (a) a greater change in absorbance per unit time, which all... | 215,347 | [
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Polymorphism of apolipoprotein E. I. Methodological aspects and diagnosis of hyperlipoproteinemia type III without ultracentrifugation. | Two methods for phenotyping apolipoprotein E are compared. One is based on preparation of VLDL by conventional ultracentrifugation, whereas the other uses heparin/Mg precipitation of VLDG. In principle, the same results were obtained by both methods. However, the group limits for the three different phenotypes Apo E-N,... | 215,358 | [
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0.04045727,
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... |
Alterations of adrenal and uterine angiotensin II receptors during variation of sodium intake and/or experimental hypertension. | 1. Angiotensin II receptors from rat adrenal gland and myometrium were studied during variation of sodium intake. 2. In both target-tissues low Na+ diet increased the number of receptors whereas a high Na+ diet did not modify the adrenocortical receptors but increased the number of uterine receptors. 3. Deoxycorticoste... | 215,372 | [
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... |
Computed tomography of the paranasal sinuses, nasopharynx, and soft tissues of the neck. | A number of cases are presented illustrating the value of computed tomography in the investigation of mass lesions involving the paranasal sinuses, nasopharynx, and soft tissues of the neck. Rapid advances in CT technology now underway will alter our approach to the investigation of certain diseases in these locations. | 215,377 | [
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Decubitus ulcers: a comparative study. | Seventeen patients with thirty-four decubitus ulcers were treated with dextranomer, collagenase, or sugar and egg white. All seven patients in the dextranomer group and two of five patients in the collagenase group improved at the end of the study, but none of the five patients on sugar and egg white treatment improved... | 215,386 | [
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Neuronal aspects of septo-hippocampal relations. | In unanaesthetized, conscious rabbits, in unstressful conditions, the neurons of the hippocampus and septum were investigated extracellularly during the presentation of a series of varied sensory stimuli. In the normal hippocampus these stimuli evoke habituating reactions of tonic (more usually, inhibitory) type in fie... | 215,389 | [
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Cardenolide analogs. An explanation for the usual properties of AY 22241. | AY 22241 (AY) was compared with digitoxigenin with respect to effects on myocardial contractility and inhibition of Na+, K+-ATPase. Ay was less potent than digitoxigenin but otherwise showed the same type of rapid onset and rapid reversal of activity usually associated with genins. Since AY is a glycoside, its behaviou... | 215,418 | [
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Two types of electronic EPSP evoked in amphibian motoneurons by ventral root stimulation. | Motoneurons of hemisected perfused frog spinal cord were used for intracellular recording of EPSP produced by ventral root stimulation. Two varieties of electrotonic VR-EPSP resistant to Ca-lack and to addition of 10 mM Mg2+ and 2 mM Mn2+ have been encountered. The early VR-EPSP started before the full somadendritic in... | 215,429 | [
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Afferent fibres from muscle receptors in the posterior nerve of the cat's knee joint. | The properties of some receptors with afferent fibres in the cat's posterior knee joint nerve have been examined, especially those discharging tonically with the joint in intermediate positions between full flexion and extension. Some of these receptors behave like muscle spindles, and respond to manoeuvres which stret... | 215,432 | [
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Influence of bromoergocryptine on estrogen-modulated prolactin receptors of mouse mammary gland. | Modulation of specific binding of prolactin to murine mammary gland as a response to treatment with various doses of estradiol benzoate (EB) was studied. Measurements of serum and pituitary levels of prolactin were also carried out simultaneously. Estradiol benzoate at a dose of 5 microgram significantly increased the ... | 215,474 | [
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A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site. | Tables specifying the frequencies, distances between and positions of all possible tetra-, penta- and hexanucleotide palindromes in phiX174 and SV40 viral DNAs were prepared by a computer search of their base sequences. A simple method based on these tables is described for identifying the sequence recognized by any sp... | 215,495 | [
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Ultrastructural and enzyme histochemical study of ovarian hilar cells in women and their relationships with sympathetic nerves. | The ultrastructure of normal ovarian hilar cells, both during the reproductive era and after the menopause, shows evidence of steroid hormone synthesis. In this respect these cells are very similar to Leydig cells in that they contain mitochondria with a dense matrix, a highly developed smooth endoplasmic reticulum, Re... | 215,506 | [
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Histological typing of nasopharyngeal carcinoma. | There is little agreement about correlations between histological type and the epidemiological and biological characteristics of NPC. This is largely due to variations in terminology and diagnostic criteria. The use of the terminology and definitions proposed by WHO would facilitate and improve international comparison... | 215,516 | [
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Interaction between Epstein-Barr virus and type-C virus in human cells. | The interaction between EBV and type-C viruses was studied in our FVNC experimental system, in which EBV and type-C viral genomes are contained in each cell. The data indicate that the human lymphoid FVNC cells are sensitive to both EBV and type-C virus exposure, showing high frequencies of induction of both repressed ... | 215,524 | [
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Cell-density-dependence for growth in agarose of two human lymphoma lines and its decrease after Epstein-Barr virus conversion. | We have compared the growth in agarose medium of two EBV-negative Burkitt lymphoma lines, BJAB and Ramos, and that of their EBV-converted derivatives. Optimal conditions for growth in agarose medium are described. The original EBV-negative lines can grow in agarose to a high efficiency, provided a critical level of cel... | 215,564 | [
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A study of 204 consecutive deliveries in Abidjan--Anthropometric data of newborns, mothers and placentas. | Anthropometric measurements of newborns, mothers and placentas were taken in 204 consecutive deliveries at Adjamé Maternity Hospital in Abidjan. Aclinical assessment of gestational age was made. Low mean birthweight can be attributed to a higher incidence of pre-term newborns in the present sample. Incidence of small f... | 215,574 | [
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Triiodothyronine (T3) concentration and therapy in autistic children. | The clinical and biochemical status of thyroid function of patients with an autistic syndrome was investigated. The study consisted of 13 patients between the ages of 7 and 21 years. There was no clinical evidence for hypothyroidism in any patient, and T3, T4, and TSH concentrations were within the normal range. Two pa... | 215,586 | [
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Acyl-Coenzyme A synthetase and fatty acid oxidation in rat liver peroxisomes. | Rat liver peroxisomes oxidized palmitate in the presence of ATP, CoA and NAD+, and the rate of palmitate oxidation exceeded that of palmitoyl-CoA oxidation. Acyl-CoA synthetase [acid: CoA ligase (AMP-forming); EC 6.2.1.3] was found in peroxisomes. The substrate specificity of the peroxisomal synthetase towards fatty ac... | 215,593 | [
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Synaptic fine structure and the termination of corticospinal fibers in the lateral basal region of the cat spinal cord. | The lateral basal region (LBR) of the spinal cord gray matter (Rexed's laminae IV-VII) by physiologic and anatomic criteria is the major terminal zone for the corticospinal (CS) tract in the cat. The neurons in this area are medium-sized with abundant spines on their dendrites. Axon terminals on the dendrites and somat... | 215,607 | [
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The relative effectiveness of commonly used disinfectants in inactivation of coxsackievirus B5. | Coxsackievirus B5 in the presence of fetal calf serum was exposed to six commonly used disinfectants for times of 10, 20 and 30 s. At the end of exposure times skim milk neutralized the disinfectant activity, with residual virus assayed by the plaque technique. The six disinfectants considered were Javex, sodium hydrox... | 215,674 | [
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Origin and transport of the A-I and arginine-rich apolipoproteins in mesenteric lymph of rats. | Transport of apolipoprotein A-I and argininerich apolipoprotein in mesenteric lymph was examined in rats given constant intraduodenal infusions of saline, glucose in saline, or emulsified fat. Lymph flow in all groups was constant from 5 to 50 hr after beginning the infusions. Lymphatic transport of triglycerides was a... | 215,684 | [
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Endogenously labeled low density lipoprotein triglyceride and apoprotein B kinetics. | The kinetics of endogenously labeled low density lipoprotein (LDL) triglycerides (TG) and apoprotein B (apoB) have been studied in four normal and in four hyperlipemic subjects using double tracers. Analysis of the data suggests that most LDL triglycerides turn over about 10 times faster than apoB (0.003/min vs. 0.0003... | 215,686 | [
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Diurnal variations in the influence of the ultimobranchial glands on calcium homeostasis in the frog (Rana pipiens). | The effect of the light-darkness cycle on the efficiency of the ultimobranchial and parathyroid glands in altering duodenal calcium transport and plasma and urinary concentrations of calcium was examined in the adult male frog (Rana pipiens). Frogs were unfed, but were allowed access to 0.05 M-CaCl2 in the surrounding ... | 215,689 | [
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Painful legs and moving toes: evidence on the site of the lesion. | A condition of painful legs with moving toes was described in 1971. Further examples of this condition are now reported, showing lesions in the posterior root ganglion, cauda equina, nerve roots, or a peripheral nerve of the lower limb. It is concluded that this syndrome is caused by a lesion of the afferent fibres of ... | 215,727 | [
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0.02235... |
Reimplantation of the ampulla of Vater after total pancreatectomy for nesidioblastosis. | Total pancreatectomy is often required to control hypoglycemic seizures in infants with nesidioblastosis. If duodenal devascularization results, reconstruction is difficult because standard biliary-intestinal anastomoses may reflux, or, in infants, become strictured. Therefore, surgeons are loath to perform such proced... | 215,736 | [
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Peptide-chain initiation with Lsc poliovirus is intrinsically more resistant to hypertonic environment than is peptide-chain initiation with Mahoney virus and deletion mutants of Mahoney virus. | Peptide-chain initiation with LSc poliovirus was more resistant to hypertonic medium than peptide-chain initiation with Mahoney poliovirus. Protein synthesis of LSc virus retained its relative resistance to high osmolarity created by the addition of excess NaCl to the medium in cells coinfected with Mahoney virus. The ... | 215,776 | [
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Comparison of the expression of the src gene of Rous sarcoma virus in vitro and in vivo. | We have compared the polypeptide products of the src gene of several strains of Rous sarcoma virus produced by in vitro translation of heat-denatured 70S virion RNA in the nuclease-treated reticulocyte lysate with those present in chick cells transformed by these viruses. We have done this by immunoprecipitation, using... | 215,787 | [
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0.2300452,
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Comparative controlled clinical trial of pivmecillinam and nalidixic acid in patients with acute simple urinary tract infections. | We performed a controlled double-blind clinical study with PMPC to investigate its therapeutic usefulness in acute simple cystitis in comparison with NA, with the results as follows: 1. In both treatment groups, PMPC proved to be remarkably more active (MIC) than NA against the bacterial isolates tested in vitro. 2. PM... | 215,795 | [
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... |
Collagen turnover and the growth state in 3T6 fibroblast cultures. | The metabolic turnover of collagen in 3T6 fibroblast cultures was studied at various stages of growth by compartmental analysis of hydroxyproline content and of the conversion of radioactive proline to hydroxyproline. Quantitative and radioactive hydroxyproline measurements were directly parallel, although the latter m... | 215,834 | [
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0.1591832,
0.2459221,
0.283356,
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-0.... |
Use of photoaffinity nucleotide analogs to determine the mechanism of ATP regulation of a membrane-bound, cAMP-activated protein kinase. | Using a radioactively tagged, photoaffinity analog of cAMP, 8-azidoadenosine-3',5'-cyclic monophosphate (8-N3 cAMP) and [gamma32P]ATP, the membrane-binding properties of both the regulatory and catalytic subunits of the cAMP-activated protein kinase of human erythrocyte membranes were investigated. [32P]8-N3 cAMP was u... | 215,838 | [
-0.2191284,
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-0.4288419,
0.115368,
-0.02680307,
0.008642783,
0.3153777,
0.2141449,
0.05944959,
0.2323664,
0.155778,
0.09645595,
0.03430244,
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0.08281729,
-0.6491358,
-0.1496671,
-0.2010432,
0.09229124,
0.772731,
0.09184163,
-0.272974,
0.... |
Congenital absence of the portal vein. | A 4 1/2-year-old girl is described in whom the portal vein is absent and the venous return from the gastrointestinal tract, spleen, and pancreas is through the left renal vein into the inferior vena cava. She had a hepatoblastoma, which was treated surgically by right hepatic lobectomy. Regeneration occurred in the rem... | 215,850 | [
0.07253218,
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-0.3838802,
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0.1084493,
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0.1732097,
0.4049902,
0.1840167,
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0.1508672,
0.1709387,
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0.1455717,
0.1602061,
-0.06864889,
0.0... |
Factors associated with utilization of the swine flu vaccination program among senior citizens in Tompkins County. | The health belief model is tested in the context of the swine flu vaccination program for senior citizens. Analysis is based on 232 responses to a questionnaire mailed to a simple random sample of 500 senior citizens in Tompkins County, New York. Social and psychologic variables identified by the model as important in ... | 215,853 | [
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0.05172495,
0.2109371,
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0.2366625,
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Fatal adenovirus type 7 pneumonia in an adult. | A case of fatal pneumonia caused by adenovirus type 7 in an adult is reported. This virus was isolated from the lung tissue, and typical inclusion bodies were found in histological sections. Death due to adenovirus pneumonia in adults appears to be extremely rare. | 215,888 | [
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0.04991014,
0.1170341,
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Recombinational repair of alkylation lesions in phage T4. II. Ethyl methanesulfonate. | Treatment of bacteriophage T4 by ethyl methanesulfonate (EMS) caused more than a doubling in recombination between two rII markers. The functions of genes 47, 46, 32, 30, uvsX and y are known to be required for genetic recombination, and mutants defective in these genes were found to be more sensitive to inactivation b... | 215,891 | [
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0.3084223,
0.02184492,
0.06420852,
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In vitro release of ACTH from dispersed rat pars intermedia cells. III. Multiple forms of ACTH biological activity. | We have reported that both an extract of rat hypothalamus-stalk-median eminence (HE) and 5-hydroxytryptamine (5-HT) will stimulate the release of ACTH biological activity from acutely, non-enzymically dispersed rat pars intermedia (PI) cells. We have also found that the ACTH activity within the PI cells is composed of ... | 215,929 | [
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0.02929841,
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Isolated trigeminal sensory neuropathy: early manifestation of mixed connective tissue disease. | A young woman with mixed connective tissue disease (MCTD) had an isolated trigeminal sensory neuropathy as an early manifestation of the disease. Raynaud phenomenon occurred almost synchronously with the onset of trigeminal neuropathy and was followed by myositis, diffuse hand swelling, synovitis, and increased ribonuc... | 215,941 | [
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0.02066237,
0.1173383,
0.06832499,
... |
Infection with paramyxoviruses stimulates synthesis of cellular polypeptides that are also stimulated in cells transformed by Rous sarcoma virus or deprived of glucose. | Infection of several types of cultured cells with the paramyxoviruses simian virus 5 and Sendai virus stimulates synthesis of four polypeptides (I-IV) with molecular weights of approximately 99,000, 97,000, 86,000, and 78,000, respectively. That these are host polypeptides encoded in cellular mRNAs has been shown by th... | 216,013 | [
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0.04452932,
0.4256462,
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-0.2681049... |
[Study of rapidity of hypotensive effects of acebutolol on non hospitalized patients (author's transl)]. | Acebutolol whose hypotensive properties are well established, appears as a most interesting beta-adrenergic blocking agent for simplification of treatment of moderate hypertension in man. Hypertensive patients likely to benefit from treatment with acebutolol can be identified within 5 days after administration of a sin... | 216,082 | [
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0.04942138,
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0.2880371,
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0.009679951,
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0... |
[Very severe haemorrhages of the lower digestive tract. The value of emergency arteriography (author's transl)]. | The authors report six cases of massive lower gastro-intestinal tract haemorrhage, diagnosed by angiography. They emphasize the value of angiography performed on an emergency basis. It is the most reliable diagnostic modality to know before surgery the precise localization of the bleeding point and sometimes its aetiol... | 216,086 | [
-0.01472503,
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0.3763945,
0.01615285,
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[Long term administration of oral prednisone or prednisolone in treatment of myasthenia gravis. Report of five cases (author's transl)]. | Five patients, four of them with severe or severe generalized myasthenia gravis, were treated by long term orally administrated prednisolone, with following results: one complete remission and two almost complete remissions (in three aged fernale patients), two substantial improvements (one in a male patient, one in a ... | 216,096 | [
-0.05361633,
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0.02424148,
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0.1179893,
0.2004589,
0.111636,
0.1202596,
0.08822722,
... |
[Alpha chain disease. Report of a case (author's transl)]. | A young patient, with alpha chain disease, is in good clinic condition, a little more than 4 years after the diagnosis. She received, during 1 year, antibiotics of tetracycline type, then antimitotics during 2 years. 9 months after antimitotics treatment was achieved, histologic signs remained unmodified, without evide... | 216,108 | [
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0.09784295,
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0.3310724,
0.4505359,
-0.1219323,
-0.1274513,... |
[Changes in the lung tissue in bronchial adenomas]. | Changes in the lung tissue in bronchial adenomas are not dependent on their histological structure, but on the anatomical form of growth, localization and duration of the existance. Both peripheral adenomas and cancer may have an asymptomatic course. In central (especially exophitic) adenomas distelectasis, atelectasis... | 216,147 | [
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0.01633194,
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0.2667785,
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0.1448188,
0.2549102,
0.06354128,
0.007994233,
0.1120924,
-0.1000561,... |
Dependence of cellular surface area on growth density of 3T3 and SV40-3T3 cells. | The cell surface areas of 3T3 and SV40-3T3 cells both for suspended and for flat cells growing on the culture substrate have been determined in dependence on growth density. The surface areas of flat 3T3 and SV40-3T3 cells turn out to be independent of growth density over a wide range. For 3T3 cells the surface area of... | 216,176 | [
-0.0988031,
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0.2194405,
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0.2801363,
0.3772426,
0.4566448,
0.03902495,
... |
Purification and some properties of collagenase proenzyme activator from rheumatoid synovial fluid. | 1. An activator of leucocyte latent collagenase has been extracted from rheumatoid synovial fluid by a preparative method consisting of six steps including precipitation by ammonium sulphate and chromatography on Sephadex G-100, QAE-Sephadex and SP-Sephadex C-50. The purification factor was nearly 1000 and the activato... | 216,183 | [
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0.02826241,
0.4069364,
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0.2743837,
0.3831203,
0.4532792,
0.04098658,
-0.1288581,
... |
Cytopathology of Paget's disease of the vulva. Diagnostic abrasive cytology. | The cytologic appearance of Paget's disease of the vulva is described, noting the similarity in appearance of the cells to those seen in Paget's disease of the nipple. The relation of this disease state to mammary involvement is emphasized by the fact that three of the five cases presenting had either a current or past... | 216,200 | [
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0.3138462,
0.4636593,
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0.5594166,
0.03298315,
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Nuclear DNA content of trophoblastic tumors. | The nuclear DNA content of trophoblasts was measured in 25 cases of normal pregnancy, 46 cases with hydatidiform mole, ten cases with destructive mole and six cases of chorionepithelioma (choriocarcinoma). In normal pregnancy, DNA distribution in syncytiotrophoblasts and cytotrophoblasts showed a sharp peak at the dipl... | 216,204 | [
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0.4807853,
0.1881308,
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-0.07... |
Immunoreactive beta-MSH in human plasma and in a corticotrophic adenoma culture medium. Its relation to the lipotrophins. | In order to characterize more accurately the relationship between immunoreactive beta-MSH ("BETA-MSH") and the lipotrophins (LPH) we attempted to investigate the gel filtration and the immunological characteristics of "beta-MSH" in the plasma of patients with Nelson's syndrome and Addison's disease as well as in the cu... | 216,210 | [
-0.3336321,
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0.3603307,
0.49194,
0.0712057,
0.01141491,
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[Peripheral nerve changes in Tangier disease. Comparative light-, electron-microscopic and cytometric studies (author's transl)]. | A sural nerve biopsy was performed in a 55-year-old male patient with Tangier disease (familial-lipoprotein deficiency). Light-microscopy showed an increase in the endoneural connective tissue and a loss of nerve fibers indicating a chronic peripheral neuropathy. Electron-microscopy revealed an accumulation of lipid dr... | 216,226 | [
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0.311578,
0.1025633,
0.3094942,
0.2556... |
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