title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
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In vitro tolerance induction of neonatal murine B cells as a probe for the study of B-cell diversification. | The susceptibility to in vitro tolerance induction has been implicated as a characteristic of B cells early in their development, since DNP-reactive B cells are tolerizable only during the first days after birth, and 25% of adult bone marrow cells are tolerizable. In the present study, a modification of the in vitro sp... | 67,178 | [
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Characterization of three antigenic particles of swine vesicular disease virus. | Three distinct particles were isolated from cell culture harvests of swine vesicular disease virus (SVDV) by sucrose and CsCl gradient centrifugation. Virions (148S), RNA-free empty capsids (81S), and a third particle (49S) also free of RNA showed immune reactivity with SVDV antiserum. The 81S and 49S particles had pol... | 67,181 | [
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Viral proteins expressed on the surface of murine leukemia cells. | Leukemic cells of AKR mice contain as constituents of their membranes the murine leukemia virus envelope protein gp70 and the precursor polyprotein of the viral internal (core) structural proteins. Both gp70 and the core polyprotein are represented on the cell surface as glycoproteins, as evidenced by incorporation of ... | 67,223 | [
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The diagnosis of bladder tumors by concentration of epithelial cells in the urine and by the help of the fluorescence property of tetracycline. | Positive results were obtained in epithelial concentrations when tetracycline was given to 50 patients with bladder tumors. The diagnosis of bladder tumors with epithelial concentration is especially helpful in patients with hematuria and pyuria. With this technique many epithelial cells radiate a bright yellow fluores... | 67,225 | [
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A relative change index in evaluating alcoholism treatment outcome. | A technique for computing a relative change index for evaluating an alcoholic's progress is presented and contrasted with less satisfactory methods of comparing data from patient intake and follow-up interviews. Applicability of the index to other treatment groups is discussed. | 67,279 | [
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Platelet activation in acute cerebral ischaemia. Serial measurements of platelet function in cerebrovascular disease. | Circulating platelet aggregates formed in vivo were serially measured, and platelet-aggregation thresholds were determined in vitro in 82 patients with acute cerebral ischaemia. The percentage of aggregated platelets was increased in 53 patients with completed stroke (30.9% +/- 2.0) and in 29 patients with transient is... | 67,334 | [
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The Tromsø heart-study. High-density lipoprotein and coronary heart-disease: a prospective case-control study. | The relationship of future clinical coronary heart-disease (C.H.D.) to the plasma-high-density-lipoprotein (H.D.L.)-cholesterol concentration has been examined in a 2-year case-control follow-up study of 6595 men aged 20-49 years living in the municipality of Tromsø, Norway. Measurements were also made of the cholester... | 67,464 | [
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Isolation of amyloid P component (protein AP) from normal serum as a calcium-dependent binding protein. | The P component of amyloid (protein AP, pentagonal unit) has been isolated from normal serum by using its hitherto undescribed calcium-dependent affinity for agarose. The presence of P component in all forms of amyloid may be due to this calcium-dependent binding to certain polyanions. | 67,487 | [
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Identification of lipids for intestinal absorption studies in resin-embedded tissue. | The use of the dye p-phenylene diamine according to Korneliussen is described for the identification of lipids in tissues embedded in resins. | 67,548 | [
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Modification of Cunningham's method for the detection of proteases in tissue sections. | A modification of Cunningham's method to show the protease activity in fresh tissue sections on gelatin film is proposed. The diazotization of the gelatin film in two successive baths of Fast blue B salt sharply increases the colour intensity of the film, improving the contrast between the intact zones and the enzymati... | 67,549 | [
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Methylene blue-azure B-eosin as a substitute for May-Grünwald-Giemsa and Jenner-Giemsa stains. | The Romanowsky-type stain of Marshall et al. [J. clin. Pathol. 28, 920--923 (1975)] is described. It possesses several advantages over the widely used May-Grünwald-Giemsa and Jenner-Giemsa stains. These include ease and speed of preparation, a shorter staining time and reproducibility of results. | 67,550 | [
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Communication in medicine: a study of how family doctors obtain information on recent advances in the treatment of rheumatic diseases. | The results of a questionnaire on how 131 family doctors in Glasgow and the West of Scotland obtain information regarding recent advances in rheumatology are described. The results show that medical journals and formal lectures and symposia are still popular. Tape/slide and television presentations were not considered ... | 67,553 | [
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[Bronchial reactivity in asthma. Immunopharmacological data]. | Some aspects of bronchomotricity in bronchial asthma, with special emphasis on pharmacological and immunological data, are presented. Personal results on beta-adrenergic blockade and stimulation on simultaneous administration of histamine and beta-blocking and stimulating drugs and on the correlation between immunologi... | 67,582 | [
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Identification of a purified complement-fixing antigen as the Epstein-Barr-virus determined nuclear antigen (EBNA) by its binding to metaphase chromosomes. | A soluble complement-fixing antigen carried by Epstein-Barr virus (EBV)-transformed human cells has been previously extracted from cell nuclei and purified by DNA-cellulose chromatography [Luka, J., Siegert, W. & Klein, G. (1977) J. Virol., in press]. On addition of this antigen to methanol/acetic acid-fixed metaph... | 67,603 | [
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A rapid alpha-fetoprotein radioimmunoassay. | A simple, reliable and inexpensive alpha-fetoprotein radioimmunoassay is described, in which polyethylene-glycol (PEG) is used as a precipitating agent of antibody-bound AFP. The concentrations of AFP found in amniotic fluid, newborn and maternal sera are in agreement with those reported by others. This assay fulfills ... | 67,626 | [
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Ultrastructure of alcoholic hyalin and fate of the affected hepatocytes. | In liver biopsy specimens, foci of satellitosis, i.e., foci of alcoholic hyalin containing hepatocytes surrounded by accumulated leukocytes, were studied by means of electron-microscopic investigation. Within satellitosis hepatocytes, the same morphologic variants of alcoholic hyalin were observed as formerly described... | 67,700 | [
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[Form and content of iodine-containing components of blood in liver pathology]. | In dynamics of development of toxic heptatitis and cirrhosis the alterations in functionality of thyroid glands were accompanied by qualitative and quantitative changes of separate iodine-containing components in blood. | 67,708 | [
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Susceptibility to experimental allergic encephalomyelitis in two strains of rats and their hybrids. | Lewis and PVG strains of rats and their F1-hybrids were challenged with guinea-pig or bovine encephalitogenic protein (EP) in Freund's complete adjuvant (FCA) to produce experimental allergic encephalomyelitis (EAE). The Lewis and F1-rats were also challenged with guinea-pig EP in FCA with a fivefold lower concentratio... | 67,742 | [
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Studies on delayed hypersensitivity in mice. I, Physicochemical and biological properties of preferential antigens for inducing delayed hypersensitivity in mice. | Variously modified protein antigens were tested by footpad assay to clarify the effect of these medications in producing delayed hypersensitivity in mice. The most potent antigen examined was carboxyl-methylated serum albumins. These antigens were highly basic proteins and hydrophobic compared with native serum protein... | 67,745 | [
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The influence of some herbicides on selected groups of microorganisms in loess and sandy soils. II. The effect of nitrification. | Studies on the effect of herbicides on nitrification in field and laboratory conditions have shown that steady use of these substances in the same field does not exert a significant effect on this process in the soil. The herbicides caused only shortlasting disturbances. Nitrification usually returned to a relative equ... | 67,749 | [
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Clinical significance of ventricular ectopic beats in the early posthospital phase of myocardial infarction. | The clinical significance of ventricular ectopic beats in the posthospital phase of myocardial infarction was studied in 272 patients aged 65 years or less who were followed up for 1 year after the infarction. Ventricular ectopic beats, identified in 6 hour electrocardiographic tape recordings, obtained before hospital... | 67,792 | [
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Pulmonary vascular disease after systemic-pulmonary arterial shunt operations. | More than 1,100 patients underwent a systemic-pulmonary arterial shunt operation at the Children's Memorial Hospital, Chicago, from 1946 to 1974. Of these patients, 294 were studied to assess the presence of pulmonary vascular disease and the time of its occurrence after surgery. They were selected either because lung ... | 67,797 | [
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Absorption from the peritoneal cavity: SEM study of the mesothelium covering the peritoneal surface of the muscular portion of the diaphragm. | Colored tracers, injected intraperitoneally in mice, are taken up by diaphragmatic lymphatics, outlining their large, terminal cisterns, the so-called lacunae. The lacunae occur exclusively on the muscular portion of the diaphragm. The mesothelium covering non-lacunar and lacunar areas of the muscular portion was exami... | 67,801 | [
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[Demonstration of the argyrophil cells of the major glands in autopsy material]. | A possibility of identifying endocrine argyrophil cells of the fundic glands of man by the method of impregnation with argentum after Grimelius in sectional material was studied. The cells detected by this method were found to be enterochromaffin-like (ECL) cells. Enterochromaffin (EC) cells in the autopsy material wer... | 67,835 | [
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Characteristics of tryptophan binding in the serum of the newborn rat. | During the very first period of postnatal life, tryptophan is almost entirely free in the serum of rats. This situation is in sharp contrast with the well-known ability of serum albumin to bind the essential amino acid in the adult. Three main factors accounted for the relative lack of binding during the early postnata... | 67,850 | [
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[Cultivation of embryonic mouse liver epithelium]. | Mouse embryo liver epithelial cells maintained in vitro were connected with each other by highly permeable cell-to-cell contacts, synthesized alpha-fetoprotein and possessed the property of contact inhibition of phagocytosis. Methods of cultivation of these cells are presented in this paper. | 67,864 | [
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Relationship of contact activation cofactor (CAC) procoagulant activity to kininogen. | Contact activation cofactor (CAC) facilitates the interaction of factors XI and XII. Patients lacking CAC have a coagulation defect and are deficient in high molecular weight kininogen. The coincidence of these two defects suggests that a single protein may be responsible for both physiologic functions. Immunologic and... | 67,865 | [
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Complete nucleotide sequence of the 5' noncoding region of rabbit beta-globin mRNA. | The 5' noncoding region sequence of rabbit beta-globin mRNA has been determined. This region is 53 nucleotides long, not including the A-U-G initiator sequence or m7Gppp "cap" structure. 32P-labeled DNA complementary to the 5' noncoding region was synthesized using reverse transcriptase with the synthetic deoxyoctanucl... | 67,896 | [
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Phase II study of 5-azacytidine in solid tumors. | A phase II study utilizing 5-azacytidine in the treatment of patients with solid tumors was carried out by the Southwest Oncology Group (SWOG-7208). Of 214 patients entered in the study 191 were eligible and 167 were evaluable. While initially they received 225 mg/m2 iv on Days 1--5 every 3 weeks because of toxicity th... | 67,893 | [
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Evolutionary relationships between gag gene-coded proteins of murine and primate endogenous type C RNA viruses. | Several low molecular weight proteins of endogenous type C viruses of the RD114/baboon group are compared with the gag gene translational products of endogenous type C viruses of murine origin. The p10 proteins of each virus group are shown to be immunologically and biochemically related, while the p12 proteins of RD11... | 67,898 | [
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Amidolytic assay of thrombin bound to alpha2-macroglobulin in plasma. | A method for the determination in plasma of alpha2-macroglogulin-bound thrombin is described. Alpha2-Macroglobulin-bound thrombin is precipitated from plasma by 13% polyethyleneglycol, and its amidolytic activity is assayed by using the chromogenic substrate benzoyl-Phe-Val-Arg-p-nitroanilide (S 2160). After thrombin a... | 67,984 | [
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0.03979113,
... |
Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. | Sixty-two explants from peripheral blood, bone marrow and cerebral fluid of children with acute lymphoblastic leukemia (ALL) and leukemic transformed non-Hodgkin lymphoma (NHL) were cultivated for at least 8 weeks. Although lymphatic cells persisted up to 16 weeks in tissue culture, no proliferation was observed in 54 ... | 68,013 | [
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The Haematoxylin-basic Fuchsin-picric acid staining reaction as a test of myocardial viability in resuscitated and preserved hearts. | In the field of the transplantation of organs, there is a great need for an in vitro test of viability which would confirm that the organ was capable of performing its normal in vivo functions. Such a test should ideally be simple, rapid and reproducible. Preliminary studies using the Haematoxylin-Basic Fuchsin-picric ... | 68,026 | [
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Ultrastructural localization of acetylcholinesterase in cultured cells. II. Cycloheximide-treated embryo muscle. | The acetylcholinesterase activity (AChE) of cultured chick embryo muscle fibers that remains after the cells have been treated with the protein synthesis inhibitor cycloheximide was examined with cytochemical stains and the electron microscope. AChE activity that decreased rapidly after addition of the inhibitor was as... | 68,070 | [
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Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains. | A comparison of homologous and heterologous rates of neutralization demonstrated that antigenic relationships of foot-and-mouth disease virus strains could be differentiated quantitatively by the kinetics of neutralization method described previously (Rwysed this way gave R values which were similar to those obtained w... | 68,071 | [
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Specific restoration of delayed hypersensitivity by lymphoid tissue extracts. | Mice lose demonstrable delayed hypersensitivity (DH) to DNFB, picryl chloride, or sheep red blood cells. Reconstitution of immune responsiveness can be accomplished by administration of cell-free lysates of spleens from mice with active DH to structurally related, but not to unrelated antigens. Peritoneal exudate cell ... | 68,074 | [
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Detection by immunofluorescence of common antigenic determinants in unrelated gram-negative bacteria and their lipopolysaccharides. | Various gram-negative bacteria were subjected to mild acid hydrolysis. The acid-treated bacteria exhibited strong cross-reactivity with fluorescein isothiocyanate-conjugated antiserum to the Re mutant of Salmonella minnesota. Hydrolyzed bacteria showed considerably stronger fluorescence than heat-treated bacteria. It i... | 68,087 | [
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Ultrastructural study of a muscle biopsy from a patient with subacute myelo-optic neuropathy. | A man, aged 46, who had been taking Clioquinol in high doses for a long period, developed a characteristic neurological syndrome of subacute myelo-optic neuropathy rather abruptly. Electron microscopical examination of the muscle biopsy, obtained five months after the onset of the disease, revealed severe degenerative ... | 68,110 | [
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Surface characterization of temporary restorations: guidelines for quality ceramics. | A technique was presented that enables a dentist to characterize a temporary restoration and its duplicate with external surface stains. This adjusted duplicate and an accompanying zone-diagrammed laboratory work authorization order will provide the dental laboratory technician with accurate information for making a po... | 68,116 | [
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... |
Ultrastrutural studies of vascular and muscular changes in ascorbic acid deficient guinea-pigs. | Disorganization of muscle structures such as fragmentation of myofilaments with a loss of contrast in the Z bands, swelling of mitochondria and glycogenic infiltration, was seen in ascorbic acid deficient guinea-pigs. Vacuolar degeneration of external and internal endothelial cell membranes with accompanying dystrophic... | 68,132 | [
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0.07374189,
-0.51... |
Post-partum transient thyrotoxicosis with painless thyroiditis. | 5 patients presented with transient thyrotoxicosis and painless thyroiditis in the post-partum period. Thyrotoxicosis developed within 1-6 months of delivery. All had small non-tender goitres. Initially, all patients had elevated values for thyroxine (T4), triiodothyronine (T3) uptake, and triiodothyronine by radioimmu... | 68,224 | [
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Relief of Sudeck's atrophy by regional intravenous guanethidine. | 17 consecutive cases of Sudeck's atrophy were treated with intravenous regional guanethidine. Results were encouraging, especially in the arm. | 68,227 | [
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Clonidine withdrawal in hypertension. Changes in blood-pressure and plasma and urinary noradrenaline. | Treatment was interrupted abruptly in 6 hypertensive patients receiving clonidine 0-45-5-4 mg daily. Blood-pressure rose to pretreatment levels within 24-48 h of withdrawal and was accompanied by insomnia, headache, flushing, sweating, and apprehension. These symptoms began 18-20 h after the last dose of clonidine. Pla... | 68,274 | [
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... |
Outbreaks of salmonella food-poisoning over a period of eight years from a common source. | In an outbreak of turkey-borne food-poisoning lasting 8 years there were 185 cases and 1 death. 10 incidents were traced to one breeding establishment. The investigation of the outbreak revealed the advantage of close medicoveterinary collaboration. | 68,344 | [
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Electrical bone-growth stimulation in an experimental model of delayed union. | An experimental model has been devised for the consistent production of delayed bone healing of the tibia in adult dogs. A double-blind trial, with bias eliminated, was used to evaluate the use of a commercially available direct-current bone-growth stimulator with this model. The stimulator produced a statistically sig... | 68,382 | [
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Evaluation of 125I-DNA for detecting anti-DNA antibodies in the diagnosis of systemic lupus erythematosus. | A 125I-DNA preparation for the detection of human anti-DNA antibodies (ADA) was evaluated as a diagnostic test for systemic lupus erythematosus (SLE). A normal range of 0-25 U/ml was established. Serum ADA level greater than 110 U/ml were diagnostic in clinically active SLE and levels greater than 45 U/ml were found in... | 68,546 | [
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0.01... |
Quantitative serological analysis of a rabbit anti-rat lymphocyte serum and preliminary biochemical characterisation of the major antigen recognised. | Rabbit antisera to rat thoracic duct lymphocytes were examined by absorption analysis with quantitative radioimmunoassays to determine the tissue distribution of the antigens recognised and the amount of antibody against different specificities. Seven of eight sera were remarkably specific for leucocytes and one serum ... | 68,560 | [
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Simplification of B-cell antisera of the Merrit system with platelets and lymphoblastoid cell lines. | Human sera with anti-HLA and anti-Merrit B-cell reactivities were absorbed with different volumes of a pool of packed outdated human platelets obtained from 237 donors. After absorption, each serum was tested against a panel of CLL cells and normal unfractionated peripheral blood lymphocytes that had given positive rea... | 68,582 | [
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HLA typing and primary cadaver graft survival. | Analysis of 463 consecutive primary cadaver renal transplants showed no influence of HLA match grade on renal allograft survival. Additional categorization according to HLA match grade and degree of presensitization again showed no correlation between match grade and graft survival. Mismatches and matches of specific a... | 68,586 | [
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... |
[Clinical significance and problems of technique in the immunochemical determination of the third complement component C 3 (beta 1 C/beta 1 A protein) (author's transl)]. | Immunochemical determination of the third complement (C) component C 3 as beta 1 C/beta 1 A protein has found extensive use in the clinical evaluation of immunopathological conditions. C 3 changes are associated with inborn C defects and acquired diseases. In the latter case, diminished C3 levels are of the greatest pr... | 68,627 | [
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Ascending aorta-pulmonary artery anastomosis for cyanotic congenital heart disease. | The course and prognosis of 208 patients with an ascending aorta to pulmonary artery anastomosis is reviewed. Mortality rate during, or within one month, of surgery was 24 per cent (50/208) and late mortality rate, prior to repair, was 10 per cent (21/208). An additional 5 per cent (10/208) died during subsequent intra... | 68,671 | [
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Chemical and immunological properties of a protein hydrolysate formula. | Clinical observation suggested that a protein hydrolysate formula designed to serve as an oral elemental diet was capable of producing untoward reactions in some children with malabsorption syndromes caused by cow milk protein allergy. An immunological study of the antigenicity of this hydrolysate was undertaken. Altho... | 68,675 | [
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0.0146588... |
The changing incidence of association of hepatitis B with hepatocellular carcinoma in California. | Two hundred seventy-nine patients who died of hepatocellular carcinoma were autopsied at Los Angeles County--USC Medical Center and the John Wesley--USC Liver Unit from 1949 through 1974, and tissues from 168 of these cases were available for staining for hepatitis B surface antigen (HBSAg). Twenty-one per cent of the ... | 68,678 | [
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[Morpholigic and physiologic repercussions of partial removal of brown adipose tissue in rats acclimated to different temperatures]. | Rats were chronically acclimated to 28 degrees C or 5 degrees C or submitted to daily variations of ambiant temperature. Ten or thirty days after removal of about 40% of the total brown adipose tissue (whole interscapular and 25% of abdominal tissues), the weight of the different pads of brown adipose tissue, thyroid a... | 68,760 | [
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Role of rat-liver alcohol dehydrogenase in the glycerol pathway to L-lactate in homogenates. | 14C-Distribution in the C3-chain of L-lactate after incubation of various 14-C-labelled prescursors ([1-14C] and [3-14C] glycerol; [3-14C] glyceraldehyde and glycerid acid; D- and L-[3-14C] serine; [1-14C] fructose and [6-14C] glucose) with homogenate showed that (1) most of the glycerol is metabolized to L-lactate via... | 68,762 | [
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... |
Nature of the stimulation of biogenesis of cholesterol in the liver by noradrenaline. | 1. Administration of noradrenaline increased the incorporation of [1-14C]acetate into hepatic sterols and the activity of liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase. 2. The stimulation was observed at short time-intervals with a maximum at 4h and was progressive with increasing concentrations of noradren... | 68,775 | [
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0.07975884,
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-0.407057,
-0.02525818,
-0.06951311,
0.3168931,
0.3466257,
0.2721055,
0.00012652... |
Monocyte-reactive antibodies in patients with systemic lupus erythematosus. | Cold-reactive antibodies cytotoxic for peripheral monocytes from more than half of normal donors were found in the sera of 2 of 25 patients with systemic lupus erythematosus (SLE) and 1 of 26 with rheumatoid arthritis (RA), and they were absent in 25 normal sera. In contrast, lymphocytotoxic activity for T or B lymphoc... | 68,777 | [
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... |
Pretreatment of hamster cells with phenethyl alcohol alters cell surface glycoproteins and inhibits vesicular stomatitis virus growth. | Chinese hamster ovary cells cultured in the presence of phenethyl alcohol exhibit obvious changes in cell surface galactose and galactosamine glycoproteins as determined by the galactose-oxidase[3H]borohydride technique and SDS gel electrophoresis. Cells pretreated with phenethyl alcohol (drug was removed before infect... | 68,807 | [
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0.008802078,
0.2663859,
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0.2092256,
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Immunochemical studies of prostatic acid phosphatase. | Immunologic specificity of the prostatic acid phosphatase (PAP) and the kinetics of the PAP-anti-PAP antibody interactions were studied in detail. Antigenic identity of electrophoretic isozymes of PAP was established in this study. Counterimmunoelectrophoretic assay, radioimmunoassay, and immunofluorescence technique w... | 68,823 | [
0.127845,
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0... |
Enzyme markers in human prostatic carcinoma. | In an effort to develop a sensitive and specific method for detecting human prostatic cancer at early stages, we have studied the isoenzyme patterns of acid phosphatase in patients' sera as well as in benign hypertrophic and cancerous prostatic tissues using isoelectric focusing techniques. At least eight acid phosphat... | 68,822 | [
-0.03940951,
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0.0758155,
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0.02676927,
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Microtubules in the neurosecretory neurones of the posterior pituitary of the rat. | The microtubules in the neurosecretory neurones of the posterior pituitary were studied using different electron microscopical techniques. Tannic acid staining indicated that the microtubules had a 13 protofilament substructure similar to that described for microtubules from other tissues and organisms; the dimensions ... | 68,834 | [
-0.07061107,
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0.2937801,
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Distribution of F-bodies, heterochromatin, and nucleolar organizers in the genome of the central mudminnow, Umbra limi. | Due to the very limited amount of knowledge available on the cytochemistry and architecture of fish chromosomes, an extensive banding study was carried out on chromosomes of the central mudminnow, Umbra limi. Through the use of fluorescent staining, C-banding, and silver staining, the chromosomes were characterized, an... | 68,856 | [
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0.652767,
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Infant day care as a treatment intervention: a follow-up comparison study. | Three-year-old children who had been in an infant day care treatment program were compared with matched normal children who were entering regular day care for the first time at age 3, to evaluate the effects of early day care intervention. All children were assessed on general pathology, play, socialization, and separa... | 68,857 | [
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0.07375172,
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Volume conduction and EEG measurements within the brain: a quantitative approach to the influence of electrical spread on the linear relationship of activity measured at different locations. | When recording referentially brain field potentials with several electrodes at relatively small tip separations, a linear relationship between the simultaneously recorded signals may arise solely as a result of volume conduction (electrical spread). A method is described to quantify the linear relationship due to elect... | 68,872 | [
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0.3515527,... |
Suppressor cell induction in vitro. III. Antigen-specific suppression by supernatants of suppressor cells. | Antigen-specific suppressor T cells induced in vitro release, after a further period of culture in vitro with antigen factors into the supernatant which have suppressor activity. These suppressor factors (SF) have the same antigen specificity as the suppressor cells (SC). SF only works on the early phase of thymus-depe... | 68,889 | [
-0.04053224,
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... |
Oncogenic interaction of carcinogenic and non-carcinogenic polycyclic aromatic hydrocarbons in mice. | To evaluate possible interactions between PAH occurring in automobile exhaust condensates with regard to their tumour forming potency, the following experiments were performed. Six different doses of benzo[a]pyrene (3-100 microgram) and of dibenzo]a,h]anthracene (2-75 microgram) and mixtures thereof were tested subcuta... | 68,911 | [
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0.3400691,
0.2909756,
0.1625454,
0.05357889,
... |
Differences in the maturation of the immune response of A- and A+ rabbits. Good and poor responders respectively for the A antigen. | The amount, avidity and specificity of anti-A antibodies produced by rabbits having the A substance in their secretions (A+) and rabbits lacking this antigen (A-), were studied with a series of antigens chemically related to the A substance. Both A- and A+ rabbits produced specific anti-A antibodies but the amount, mat... | 68,927 | [
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0.3930566,
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Transfer of delayed hypersensitivity in mice to microbial antigens with dialyzable transfer factor. | Dialyzable Lawrence-type transfer factor was prepared from the spleen cells of CF1 mice inoculated with Coccidioides immitis- and Candida albicans-killed vaccines and with live Mycobacterium tuberculosis vaccine (BCG). These preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as m... | 68,930 | [
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0.1615723,
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0.108272... |
Detergent solubilization, purification, and separation of specificities of HLA antigens from a cultured human lymphoblastoid line, RPMI 4265. | HLA antigens have been purified to homogeneity after detergent solubilization from RPMI 4265, a human lymphoblastoid line. The inhibition of cytotoxicity assay for HLA antigen was modified, using preincubation with bovine serum albumin of antigen samples containing detergent to prevent lysis of target cells by detergen... | 68,955 | [
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0.4591846,
0.2307774,
0.5249764,
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0.327... |
Immunological and biochemical studies of collagen type transition during in vitro chrondrogenesis of chick limb mesodermal cells. | This work describes an approach to monitor chondrogenesis of stage-24 chick limb mesodermal cells in vitro by analyzing the onset of type II collagen synthesis with carboxymethyl-cellulose chromatography, immunofluorescence, and radioimmunoassay. This procedure allowed specific and quantitative determination of chondro... | 68,959 | [
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0.009581114,
... |
Immunologic tolerance to heterologous immunoglobulin: its relation to in vitro filtration by macrophages. | The cellular and molecular basis for the difference in ability of BCG to induce tolerance in BALB/c and DBA/2 mice has been examined by in vitro biofiltration. It was found that incubation with the adherent cells from BALB/c but not DBA/2 spleens could remove the material from BGG which inhibited tolerance induction in... | 68,973 | [
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0.09711332,
0.1942596,
0.1152501,
0.03651056,
-0... |
The influence of oestradiol on the metabolism of androgens by human prostatic tissue. | The uptake and metabolism of testosterone, androstenedione and 5alpha-dihydrotestosterone (DHT) by human benign hyperplastic prostates and prostatic carcinomas have been measured in organ culture. DHT was a major metabolite of both testosterone and androstenedione in the benign tissue and the androstanediols were the p... | 68,984 | [
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0.4871065,
0.5191271,
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B-cell tolerance. III. Effect of papain-mediated cleavage of cell surface IgD on tolerance susceptibility of murine B cells. | Under defined conditions, papain removes IgD from cells while leaving IgM, H-2, Ia, Lyb-2, and complement receptor intact. The effect of such treatment with papain on the induction of tolerance in murine splenic B cells was determined in an in vitro system. Treatment of the cells with papain has no effect on subsequent... | 68,987 | [
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Regulatory functions of hapten-reactive helper and suppressor T lymphocytes. I. Detection and characterization of hapten-reactive suppressor T-cell activity in mice immunized with hapten-isologous protein conjugate. | Helper and suppressor T-cell activities were detected simultaneously in the spleen cells of mice immunized with para-azobenzoate (PAB)-mouse gammaglobulin (MGG). Dinitrophenyl (DNP)-specific B cells were raised by immunization with DNP-keyhole limpet hemocyanin (KLH) and used as the indicator B-cell population. The hel... | 68,994 | [
-0.04061111,
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0.158231,
... |
In vitro generation of tumor-specific cytotoxic lymphocytes. Secondary allogeneic mixed tumor lymphocyte culture of normal murine spleen cells. | In vivo or in vitro immunity to murine leukemia virus (MuLV)-induced leukemia cells which do not effectively produce virus, has been difficult to demonstrate. Because immunizations with allogeneic murine leukemia cells have been used to confer syngeneic tumor immunity to virus- producing cells, we attempted to generate... | 69,003 | [
-0.2369004,
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0.2833706,
0.3101639,
0.2108953,
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... |
Drug risks in pregnancy revisited. | In the important interface of assuring safe care for the pregnant mother and her developing child that falls to the family physician, current knowledge of drug risks in pregnancy is essential. A recent survey showed that an average of 4.5 drugs are taken throughout the pregnancy. Eighty percent are self-prescribed, and... | 69,010 | [
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0.09281235,
-0.1263844,... |
Antimicrobial action of compound 48/80 against protozoa, bacteria, and fungi. | Compound 48/80 inhibited the growth of protozoa, bacteria, and fungi but had no effect on the multiplication of viruses. All susceptible organisms were inhibited by 10 microgram/ml of crude compound 48/80, and some were inhibited by as little as 0.1 microgram/ml. Against Tetrahymena pyriformis, this drug was seven time... | 69,023 | [
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0.3806085,
0.02542368,
0.1807262,
-0.2573228,
0.... |
Immunisation with a polyvalent pneumococcal vaccine. Reduction of adult respiratory mortality in a New Guinea Highlands community. | A double-blind controlled trial of a 14-valent pneumococcal polysaccharide vaccine was carried out in 11 958 adults at Tari in the Papua New Guinea Highlands. Pneumococcal infection, confirmed by blood-culture and lung aspirate, was less in the vaccinated group by 84%. Mortality from pneumonia was less by 44%. | 69,058 | [
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0.2257326,
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0.3762367,
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Diagnosis of hyperthyroidism when serum-thyroxine alone is raised. | 31 patients admitted to hospital with different non-thyroidal intercurrent diseases were found to have raised total serum-thyroxine (T4) and free T4 together with normal serum-triiodothyronine (T3) concentrations. At admission none was clinically hyperthyroid. Patients were assigned to 3 groups according to clinical co... | 69,145 | [
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0.03199544,
0.3427866,
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Effect of insulin antibodies on free and total plasma-insulin. | Insulin administration often causes the development of insulin antibodies. Plasma insulin-binding capacity was assessed and free and total plasma insulin were measured in 96 insulin-dependent diabetic patients. Patients with the highest insulin binding capacity not only had the highest total insulin but also had the lo... | 69,146 | [
0.001819657,
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0.1329979,
0.2898026,
0.1503796,
0.09628708... |
Contacts between young patients with Hodgkin's disease. A case-control study. | To investigate the possibility that Hodgkin's disease (H.D.) may be transmitted from person to person, a case-control study was conducted among 87 of the 97 H.D. patients diagnosed under the age of 40 years in the period 1962-71, resident at the time of diagnosis in a defined area around Oxford. For each of the 87 H.D.... | 69,147 | [
-0.217794,
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0.01895179,
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0.1075319,
0.3355908,
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... |
Mutagenic and lethal effects of alpha-benzene hexachloride, dibutyl phthalate and trichloroethylene in Saccharomyces cerevisiae. | Saccharomyces cerevisiae strain XV185-14C for reversion studies was used to investigate the genetic activity of alpha-benzene hexachloride dibutyl phthalate and trichloroethylene. The results indicate that none of the three compounds was genetically active when yeast cells were treated in phosphate buffer (pH 7.0) in t... | 69,268 | [
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0.08908489,
0.1869907,
0.07698633,
0.3578938,
0.1168634,
-0.1401... |
[Immunohistochemical study of alpha-fetoprotein in the postnatal ontogeny of mice with a varying sensitivity to liver carcinogens]. | The ontogenesis of alpha-fetoprotein (AFP) was studied in the C57BL, CC57BR, C3H/He, A/He and DD mice during the first 4 weeks of life. The AFP hepatocytes were determined by indirect immunofluorescent staining of liver cells isolated with sodium tetraphenyl borate; the content of AFP in blood was determined by the met... | 69,282 | [
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0.1063785,
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0.3935342,
0.3443162,
0.01537356,
-0.05047619... |
Fine particles of cotton dust influence histamine release. | Cotton dust particle distribution in respiratory airways was measured to determine if specific size particles of the heterogeneous dust are concentrated as a function of airway diameter. Previously reported work indicated that the fine particles are responsible for the decrement in pulmonary function which occurs subse... | 69,279 | [
0.0114255,
-0.07368359,
-0.4071923,
0.01672843,
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0.1011756,
0.01504191,
0.1056849,
0.1776834,
0.05948149,
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0.08189306,
0.02106557,
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0.209184,
0.1860954,
0.107826,
-0.1417803,
0.309617,
0.18792,
-0.... |
[Antithyroid activity of various newly synthesized 1,3-thiazine derivatives]. | Experiments were performed on rats. It was revealed that three newly-synthesized 1,3-thiazine derivative (1,3-thiazandithion-2,4, 1,3-thiazanthion-2-on-4-thiosemicarbazone-4, and 1,3-thiazandion-2,4) possessed and antithyroidal action in doses equal to 15% of LD50; in the two former preparations it was accompanied by a... | 69,301 | [
-0.3261776,
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0.01453126,
0.1020266,
0.0127987,
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0.1288124,
0.1837874,
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0.2985684,
0.01140618,
0.2131844,
0.2597018,
0.160399,
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0.... |
The present status of cytochemistry in the diagnosis of haematological malignancy. | Newer concepts in the application of cytochemistry to the diagnosis of haematological malignancies are discussed. The underlying physiological principles and location of the various enzymes are indicated. Older stains, once thought to be diagnostic, have been shown to be nonspecific. Newer, more accurate stains are now... | 69,324 | [
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0.3351085,
0.2819767,
0.2508245,
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0.2... |
A silver impregnation method for nervous tissue suitable for routine use with mounted sections. | A simple, reliable silver impregnation method for nervous tissue is described for tissues fixed in various fixatives including formalin, Bouin, and Susa. Sections are impregnated in a solution containing 1 g Protargol, 2 ml of a 1% Cu(NO3)2 solution, 2 ml of a 1% AgNO3 solution, and 2-4 drops 30% H2O2 in 100 ml distill... | 69,340 | [
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0.06818336,
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0.01087085,
0.04009423,
0.001901913,
-0.17239... |
Radioimmunoassays of ethinyl-norgestrienone (R-2323) and medroxyprogesterone acetate (MPA) and their clinical applicability. | Radioimmunoassays for 2 synthetic progestins (Ethinyl-norgestrienone, R 2323 and medroxyprogesterone acetate, MPA) are demonstrated. 10 patients aged 31 to 72 years were treated with ethinyl-norgestrienone with different schedules and 3 men suffering from benign prostatic hypertrophy were treated with medroxygesterone ... | 69,351 | [
-0.009455922,
0.08499989,
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0.3796048,
0.09744915,
0.1749936,
0.2933473,
0.2564555,
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0.2505809,
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0.1912114,
0.5918544,
0.1208784,
-0.2248318,
... |
[Control of therapy for hyperthyroidism. Comparison of in-vitro and in-vivo methods]. | 55 patients with treated hyperthyroidism (thyreostatics) underwent a T3 suppression test and a TRH stimulation test. 60% of the patients had a positive and 40% a negative T3 suppression test. While patients with a positive T3 suppression test always showed a positive TRH test, the behaviour of the TRH test in negative ... | 69,363 | [
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0.01128507,
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0.05186674,
0.05408163,
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0.1156681,
0.1902235,
0.1037579,
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-0.2250047,
-0.02313303,
-0.273897,
0.1994032,
0.2026022,
0.03278989,
-0.08206514... |
[Arthropathies in intestinal diseases]. | Together with gastroenterologists rheumatologists describe diagnostically significant disturbances of the supporting apparatus and the locomotor system which frequently occur in ulcerous colitis, enterocolitis regionalis Crohn, Whipple's disease and non-tropical sprue. Important conclusion for the diagnostic approach s... | 69,364 | [
-0.03012907,
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0.09015853,
0.02054178,
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0.03275912,
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0.342319,
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0.2052983,
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0.1470963,
-0.1668871,
0.08932005,
-0.04382993,
-0.1115814,
0.04095327... |
[Evaluation of the efficacy of anti-arrhythmic drugs using a computer]. | To palliate the deficiencies of the oscilloscopic monitoring of arrhythmias, an automatic system has been developed. It has been used during more than one year time in the Coronary Unit for supervision of patients and for study of the pharmacological effects of antiarrhythmic drugs. | 69,379 | [
0.03280581,
0.06034171,
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-0.176139,
-0.005143425,
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-0.1469676,
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0.2481798,
-0.3411437,
-0.003386506,
0.2928929,
0.1843746,
0.1608775,
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-0.5022863,
0.2533618,
-0.06789668,
0.09461439,
0.3185908,
-0.04443414,
-0.14630... |
[Choice of surgical procedure in the treatment of stomach cancer]. | There are two demands that have to be satisfied in the treatment of gastric carcinoma: 1) eliminate the tumor radically and 2) to maintain the physiological function of the digestive tract. Out of radical methods this is enabled by subtotal and total gastric resections of Billoroth I type. Especially satisfying among t... | 69,380 | [
-0.166532,
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0.1886108,
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0.02691457,
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0.4110327,
0.09915892,
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-0.2989775,
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0.332903,
-0.03800995,
0.1591051,
0.1604238,
-0.1626405,
... |
Peripheral immunofluorescence of hepatocytes. Relation to smooth muscle antibodies and bile canaliculi. | By means of indirect immunofluorescence microscopy, a peripheral fluorescence reaction with hepatocytes was found, surrounding the entire cell, with sera from patients with cancer and acute hepatitis and from normal blood donors. It was proved that this reaction was not related to bile canaliculi, contrary to bile cana... | 69,387 | [
-0.06092246,
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0.1695619,
0.04484156,
0.04157401,
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0.5150235,
0.027191,
0.1539149,
0.2247608,
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0.03026959,
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0.2085344,
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0.110644,
0.3251225,
-0.2837891,
-0.03754351,
0.... |
[Age-dependence of the cellular immune reaction in childhood. 2. Studies on children with acute lymphatic leukemias]. | Results of studies made using the lymphocyte transformation test for leukemic children showed that an evaluation of cellular immunoreactions subsequent to the administration of szecific and nonspecific antigens as well as in the mixed culture is inadequate to recognize the characteristics of age-specific changes. A com... | 69,391 | [
0.1181701,
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0.3473616,
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0.2736751,
0.0320981,
0.1569337,
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0.3240715,
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0.5814461,
0.5602261,
-0.01949561,
-0.05628332,
... |
[The influence of 6-mercaptopurine on the humoral immune response in guinea pig. 1. The importance of antigen and adjuvans (author's transl)]. | Following an intradermal immunization of guinea pigs with dinitrophenylated bovine gamma globulin (DNP23-BGG), human serum albumin (HSA) and trinitrophenylated sheep red blood cells (TNP-SRBC) emulsified in complete or in incomplete Freund's adjuvans the effectivity of 6-mercaptopurine (6-MP) on the formation of specif... | 69,395 | [
0.207947,
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-0.0009004152,
0.1317096,
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0.1343314,
0.04008219,
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0.2809052,
0.4378178,
0.1380278,
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0.3367926,
0.2339448,
0.5192364,
0.08465081,
0.1232558,
-0.2109275,
-0... |
Detection of circulating tumor antigens. | A wide variety of malignancies have associated tumor antigens. Two which have proven useful in the clinical detection and management of cancer are alphafetoprotein (AFP) and the carcinoembryonic antigen (CEA). These materials have been determined both by radioimmunoassay and by a newer technique employing enzymes to re... | 69,486 | [
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-0.04955858,
-0.1794786,
-0.489421,
-0.2394761,
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0.2388808,
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0.3197864,
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0.379519,
0.2788562,
-0.1156921,
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0.1213563,
0.1644864,
0.5013903,
0.3252211,
0.04542023,
-0.2413688,
0.... |
Suppression of tumor-specific cell-mediated cytotoxicity by immunoregulatory alpha-globulin and by immunoregulatory alpha-globulin-like peptides from cancer patients. | The suppression of tumor-specific cell-mediated cytotoxicity by human immunoregulatory alpha-globulin (IRA), by a peptide fraction derived from IRA, and by IRA-like peptides from the serum of cancer patients was studied in a syngeneic murine tumor-host system. Splenic lymphocytes from tumor-immunized mice were cytotoxi... | 69,489 | [
-0.3086858,
-0.3369551,
-0.4269806,
-0.3640033,
0.2072948,
-0.09919794,
-0.04434936,
0.2503099,
0.2748352,
0.2385077,
0.001893701,
0.3820814,
0.03741435,
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0.1796431,
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-0.157956,
0.0873168,
0.5494322,
0.2256808,
0.1441626,
-0.08950783,
0... |
Serum beta2-microglobulin in controls and cancer patients. | Serum beta2-microglobulin levels have been measured in 210 cancer and control patients to assess the significance of this investigation in cancer patients. Subjects studied included patients with breast and gastrointestinal cancer, corresponding control patients in both categories, and healthy volunteers. The compositi... | 69,507 | [
-0.2212322,
0.07466152,
-0.5309424,
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-0.238103,
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0.04683786,
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0.08295637,
0.1601689,
0.157171,
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-0.1981497,
0.04085073,
0.1989233,
0.2299523,
0.09787192,
-0.1719194,... |
Duration of paradoxical sleep episodes. A quantitative and pattern analysis of reticular multi-unit activity in the cat. | This study was aimed at the possible relationships between the level of brain activation in PS and the duration of episodes. Multi-unit activities from 16 recording sites in the mesencephalic and pontine structures were quantitatively evaluated in five cats and the influence of electrode localization and episode durati... | 69,536 | [
0.2319639,
0.2563569,
-0.2483041,
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0.2885146,
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0.05970005,
0.03995371,
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0.2423636,
0.2038013,
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0.08857659,
0.1748829,
0.2087096,
-0... |
[Relationship between the endocochlear potential and metabolism in the vascular stria of the inner ear]. | Effects of dihydrostreptomycin, etacrine acid and ATPh on the endocochlear potential in guinea-pigs depended on the dosage and means of administration. Dihydrostreptomycin suppressed the potential only when perfusing the perilymphatic space or being microinjected into the endolymphatic duct. I. v. administration of eta... | 69,557 | [
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0.1427606,
0.1829883,
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0.2543501,
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-0.5379944,
-0.03797238,
-0.2494429,
0.1275871,
0.4354398,
0.07270622,
0.08531412,
... |
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