title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
|---|---|---|---|
[Immunodiffusion tests in gel media with the addition of polyethyleneglycol 6000 for the serodiagnosis of mycoses]. | Different immunodiffusion techniques with and without the addition of polyetilenglycol 6000 (PEG), were studied to determine its effect on the sensitivity of these reactions. One hundred thirteen sera from patients who suffered or had suffered deep mycoses (paracoccidioidomycosis: 49, histoplasmosis: 25, aspergillosis: 25, candidiasis: 8 and coccidioidomycosis: 6) were examined by the quantitative Ouchterlony's immunodiffusion procedure. Regular medium and media with 2% and 4% PEG were used. Eighty two out of the one hundred thirteen sera were positive for the regular medium and 91 for the medium containing 2% of PEG; furthermore, an increase of 1 or 2 two fold dilutions in the titers was observed in 40% of the sera, for the later media. Twenty one sera from aspergillosis cases were examined by agarose gel immunoelectrophoresis, 80% had more precipitin bands in the medium with 2% of PEG. Thirty four serum samples of patients suffering aspergillosis, paracoccidioidomycosis and histoplasmosis were studied using the agarose electroosmophoresis with the secondary immunodiffusion test. An increase in the number of the anodic bands were observed in 55% while 64% presented more catodic bands, when the PEG medium was used. This results would indicate that the addition of 2% PEG 6000 to the regular medium improves the sensitivity of the immunodiffusion tests for mycoses. | 116,327 | [
-0.1223284,
-0.5213802,
0.2882884,
0.2617213,
0.3236149,
0.04559435,
-0.3150927,
-0.04372672,
-0.03174458,
0.3276988,
0.03128117,
0.09442864,
-0.02084047,
-0.07161172,
-0.2979663,
-0.2143193,
-0.554343,
0.07615606,
-0.2793297,
0.1837329,
0.285606,
-0.1068348,
0.1139628,
... |
[Immunochemical characterization of rabies virus: determination of precipitable antigens]. | Immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. The passive haemagglutination test with group "O" human red cells treated with tannic acid, was used as control test. The study was done with brain suspensions of rabies virus infected mice (CVS strain). Mouse and horse rabies antisera were used. The analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. The same two fractions were detected when the antigens were heated. Experiments with brain extracts centrifuged at high speed, suggested that the antigenic fractions are of the "soluble" type. | 116,328 | [
-0.1788617,
0.0695401,
0.003103461,
0.2210105,
0.225131,
-0.1121658,
-0.006875663,
0.02530949,
-0.1629789,
-0.06233031,
0.08532029,
0.3993294,
-0.02455946,
-0.275776,
-0.7013736,
-0.1247804,
-0.2274618,
0.08643002,
0.2433642,
0.1162845,
0.2054716,
0.1099381,
-0.3144371,
... |
Augmentation of hemagglutination by low ionic conditions. | Short incubation at 37 C, 80 per cent reduction in ionic concentration and removal of liquid phases after each reaction step, provided the basis for the construction of four new serologic tests for alloantibodies to human erythrocytes. In the first, the incubation fluid was replaced with protamine sulfate to aggregate intensely the evaluated red blood cells. After dispersal by phosphate buffer, residual antibody mediated agglutination could be discerned. As a second method, this low ionic polycation (LIP) test was followed by a normal ionic IgG antiglobulin test (LIP-AGT). A third method employed low ionic washing of erythrocytes and low ionic antiglobulin serum (LIAGT). Finally, a modified LIP test was conducted entirely under low ionic conditions and followed by a low ionic antiglobulin test (modified LIP-AGT). LIP, LIP-AGT and LIAGT were successfully employed for all routine blood bank serology tests. Their sensitivity and impact on blood bank performance are described. | 116,397 | [
0.06254867,
-0.07230958,
-0.3862667,
0.02275712,
-0.07548141,
0.007175177,
-0.1236899,
0.2394766,
0.1223608,
0.06678217,
0.2412998,
0.1952068,
-0.01552123,
-0.07653058,
0.02320904,
-0.000228028,
-0.1846593,
-0.1080954,
0.2735312,
0.2801529,
0.3126349,
0.1297076,
-0.202233... |
The gangliosidoses: comparative features and research applications. | Ganglioside storage diseases are inherited defects of lysosomal hydrolases that result in intralysosomal accumulation of gangliosides and other complex metabolites. Gangliosidoses occur in man, cats, cattle, dogs and swine. In all species, these diseases are characterized clinically by relentlessly progressive neurological deterioration. Lysosomal hypertrophy with characteristic ultrastructural inclusions occur in neurons, endothelial and other cells. Definitive diagnosis requires biochemical identification of the storage product and enzyme deficiency. Gangliosidoses in animals are important models of human lysosomal diseases and may be a significant complication in the maintenance of certain purebred stocks of domestic animals. | 116,415 | [
-0.04765981,
-0.07635855,
0.08321952,
-0.2184204,
0.1281733,
-0.4431249,
0.2088517,
0.009918591,
-0.3408304,
0.08458767,
-0.1402709,
-0.1169786,
-0.18148,
-0.03684226,
-0.4254259,
0.2618865,
-0.4203058,
0.3025735,
0.1555589,
0.1257183,
-0.1081504,
0.6946843,
-0.09847084,
... |
Ventilation-perfusion studies in suspected pulmonary embolism. | The results of ventilation-perfusion (V-Q) imaging and pulmonary angiography were retrospectively analyzed in 146 patients with suspected pulmonary embolism (PE) to define the frequency of PE associated with various scintigraphic patterns. When the radionuclide images demonstrated at least two moderate-sized or one large area of V-Q mismatch, the probability of PE was 92%. However, only one of three patients with a single moderate-sized V-Q mismatch had PE, while small V-Q mismatches were not associated with PE in any of 19 patients. Matched V-Q abnormalities in lung regions that were radiographically normal were infrequently due to PE (4.8%). When a perfusion defect was substantially smaller than a corresponding radiographic abnormality, the frequency of PE was low (7.7%). Conversely, when a perfusion defect was substantially larger than the corresponding radiographic abnormality, there was a high probability of PE (87%). Matched perfusion and radiographic abnormalities indicated an intermediate probability of PE (27%). Patients with suspected pulmonary embolism may be classified into groups with low, intermediate, or high probability of pulmonary embolism on the basis of size and number of perfusion defects and a careful comparison of perfusion defects with ventilatory and radiographic findings. | 116,491 | [
-0.1049228,
0.2699959,
-0.2298237,
-0.006673114,
-0.005221924,
-0.003566069,
-0.1960697,
0.04253431,
-0.1651131,
0.03739158,
0.05713161,
0.3027709,
-0.3246051,
0.0213482,
0.125172,
-0.2485614,
-0.1931434,
0.2928716,
0.4171965,
-0.1886227,
0.1015633,
0.02092356,
-0.0489899... |
Evaluation of contrast markers for CT aspiration biopsy. | Precision percutaneous needle aspiration biopsy under guidance of computed tomography is facilitated by injection of a small amount of contrast material during the biopsy procedure. CT scan immediately after biopsy clearly documents the biopsy site. There was no adverse effect of the contrast agents on the quality of staining or preservation of cellular architecture. To the contrary, the aspirates obtained after contrast injection were generally better preserved and better stained than the control specimens. This may be due to a decrease in drying, with the more fluid aspirates obtained after contrast injection. | 116,500 | [
-0.07922039,
0.2419828,
-0.2008954,
-0.3598469,
-0.05680848,
-0.1854567,
-0.2827361,
-0.01249633,
0.1379694,
0.0483517,
-0.05937846,
0.02603092,
0.01904124,
-0.0781971,
-0.2441459,
-0.3740695,
0.003744717,
0.03265337,
-0.003997406,
0.2397301,
0.04512088,
0.1971042,
-0.233... |
Three methods for determining anteversion and retroversion of a total hip prosthesis. | Three new methods to assess the degree of anteversion or retroversion of the femoral component of a total hip prosthesis are described. Compared with previously described methods, the new methods make few physical demands on the recently operated patient. The only requirement is that the affected femur be held in a neutral position parallel to the x-ray table. The methods are relatively easy to perform and are accurate and objective. | 116,505 | [
-0.1974923,
0.4137745,
-0.2026784,
0.04727088,
-0.1413197,
-0.2203616,
-0.05031223,
0.1261991,
-0.06634118,
0.2507156,
0.06139351,
-0.1225919,
-0.1426378,
-0.213204,
-0.2488329,
-0.1919814,
-0.1931298,
0.2664388,
-0.3736832,
0.06104282,
0.3344503,
0.03279617,
0.1075564,
... |
Folacin deficiency and requirement in the squirrel monkey (Saimiri sciuresus). | Acute folacin deficiency was studied in eight young squirrel monkeys (Saimiri sciureus). Half of the animals were fed a semipurified deficient diet (no added folic acid) and half were fed a control diet (0.84 mg of added folic acid per kilogram of dry diet). Monkeys fed the deficient diet lost weight and suffered from diarrhea and dehydration leading to the death of one of the animals after 6 weeks. Folacin deficiency also was studied in six older animals fed diets containing varying levels of added folic acid. Monkeys fed diets containing 0.14 or 0.27 mg of added folic acid per kilogram of dry diet slowly developed alopecia, a scaly dermatitis, and a mild macrocytic anemia. When these animals were fed the deficient diet, they lost weight rapidly, the alopecia and dermatitis worsened, excretion of formiminoglutamic acid in the urine increased, and a severe megaloblastic anemia with profound intramedullary hemolysis developed. Deficient monkeys had low plasma and red blood cell folacin values but maintained normal plasma vitamin B12 values. Repletion of the animals fed the deficient diet with injections of folic acid reversed both the hematological and physical deterioration. The folacin requirement for maintenance of body weight in these animals was 28 micrograms of total folacin per kilogram of body weight per day. More than 75 micrograms of total folacin per kilogram of body weight/day may be needed to assure growth and normal hematological parameters and bone marrow cytology. | 116,540 | [
0.03005548,
-0.2706699,
-0.2372313,
-0.3446234,
0.2547089,
-0.1973322,
0.3348613,
-0.06706818,
-0.4089182,
-0.2983945,
0.2173488,
0.3044884,
-0.09303571,
0.3900806,
0.1061248,
0.1052645,
-0.2083844,
0.1503153,
-0.08531576,
0.2776486,
-0.06207633,
0.1917849,
-0.03694031,
... |
Infection rates of Ascocystis-infected Aedes triseriatus following ingestion of La Crosse virus by the larvae. | The La Crosse (LAC) virus infection rate of Aedes triseriatus larvae that ingest LAC virus does not appear to be increased by concomitant infection of larvae by the gregarine parasite, Ascocystis barretti. Infection rates ranged only from 0--2.6% in adult Ae. triseriatus reared from groups of A. barretti-infected larvae that had ingested LAC virus (California encephalitis group) at dosages of 2.0--7.7 log10 SMICLD50/ml. Females resulting from orally infected larvae transmitted LAC virus to suckling mice. Larvae that were infected with A. barretti and devoured carcasses of adult mosquitoes containing 4.7 log10 SMICLD50/ml failed to become infected. A. barretti spores developing in transovarially infected mosquitoes did not harbor LAC virus; thus, A. barretti does not appear to be a mechanism for virus dispersal. | 116,554 | [
-0.3558527,
-0.2970512,
0.02030358,
-0.08500258,
0.0750648,
-0.4152075,
-0.2655632,
-0.2694154,
0.2550548,
-0.1731244,
-0.01818126,
0.2045794,
0.3730853,
-0.002337608,
-0.499596,
-0.2430895,
-0.6811156,
0.2140153,
0.2704546,
0.3699736,
-0.1161617,
-0.008205261,
-0.0663143... |
Plasmodium fragile and Macaca mulatta monkeys as a model system for the study of malaria vaccines. | Pladmodium fragile and the Macaca mulatta monkey are presented as a model system for the study of malarial vaccines. Four animals were immunized with culture-grown P. fragile merozoites and subsequently challenged with culture-produced parasites. One animal failed to develop a detectable parasitemia following primary challenge. Two other immunized animals had primary infections which were short-term. the parasitemias in these three monkeys following secondary challenge were short-term and the immunity was apparently sterilizing. The fourth immunized monkeys had recrudescences of the primary and secondary infection but differed markedly from the four control animals. Indirect fluorescent antibody titers increased as a result of the immunization and were indicative of the level of immunity. Because of the many similarities to the human-P. falciparum model system, the P. fragile-M. mulatta system appears to be particularly well suited for a number of malaria vaccine studies. | 116,557 | [
-0.001697026,
-0.09103774,
-0.1094496,
-0.09543945,
-0.03975811,
0.01752631,
-0.05248762,
0.3382857,
0.04944114,
0.01125348,
0.1017799,
0.177455,
0.2350207,
-0.01950764,
0.005951417,
-0.3614451,
-0.264302,
0.04686852,
-0.2076315,
0.3970599,
0.5666459,
0.01691229,
-0.21245... |
Immunogenetic studies of rhesus monkeys. 6. Absence of the human Xga blood group on rhesus erythrocytes. | It was not possible to demonstrate Xga on the erythrocytes of any of 140 rhesus monkeys of both sexes tested with human antiserum rendered specific for Xga by absorption. | 116,572 | [
0.02283668,
-0.2303493,
-0.2406372,
0.01342139,
-0.1567048,
-0.2059937,
-0.3520471,
-0.13255,
0.04045347,
-0.0367474,
0.1842271,
0.2653272,
0.08479816,
-0.1208316,
-0.7781563,
-0.2647933,
-0.4364708,
0.03329638,
-0.1757119,
0.03336896,
0.3014622,
0.1867003,
-0.03547189,
... |
The absence of protein--sparing effects utilizing crystalline amino acids in stressed patients. | The protein-sparing effects of the peripheral infusion of crystalline amino acids (PAA) was studied metabolically in selected surgical patients subjected to various degrees of stress. Twenty-one patients (sixteen cancer patients receiving chemotherapy and/or radiotherapy, three with major abdominal traumatic injuries and four with paralytic ileus) were infused with 2 1/24 hours of a solution of 4.2% Travasol amino acids with only 5% glucose as a source of nonprotein calories. One-half of the cancer patients were also allowed ad libitum oral intake of a regular hospital diet or Vivonex-HN. The nutritional status was evaluated by measuring changes in body weight, serum albumin levels and nitrogen balance. Body weight decreased in only the trauma patients. When these solutions were the sole source of nutrients all patients were in negative nitrogen balance and had significant decreases in their serum albumin levels. Serum albumin levels were preserved only when extra sources of calories were provided. The infusion of the crystalline amino acids without adequate levels of nonprotein energy did not conserve protein in these stressed patients. | 116,604 | [
-0.1923641,
-0.5344974,
-0.4655151,
-0.2728188,
-0.1634039,
-0.1553327,
-0.04144118,
0.1337709,
-0.0001221181,
0.06744394,
-0.05654452,
-0.2366177,
0.3017205,
-0.1694423,
-0.03386495,
-0.1439338,
-0.3048767,
-0.3897922,
-0.3736212,
0.3277528,
0.1439964,
-0.0305895,
-0.140... |
[Outcome of myocardial infarctions complicated by heart conduction disorders in the acute phase]. | Of 945 patients hospitalised for myocardial infarction between January 1st 1972 and December 31st 1975, 40 with anterior myocardial infarction (Group I-A) and 53 with posterior myocardial infarction (Group II-A) were complicated by atrioventricular and/or intraventricular arrhythmias. The average follow up period is now of 48 months (range 24 to 78 months). Their outcome was compared to two control groups of 50 anterior myocardial infarctions (Group I-B) and 50 posterior myocardial infarctions (Group II-B) uncomplicated by arrhythmias in the acute phase. The immediate (10%) and secondary (30%) mortality was identical in the two groups II-A and II-B with posterior wall necrosis. The immediate (32%) and secondary (40%) mortality in Group I-A was much higher than in Group I-B (22% and 28% respectively). Sudden death was the most frequent form of demise in all groups (I-A, II-A, II-B) except Group I-B in which heart failure predominated. Death occured earlier in Group I-A than in the control Group II-B. These results pose the problem of the indication of prophylactic permanent pacing to decrease the incidence of sudden death. | 116,618 | [
-0.3737064,
0.06609586,
-0.0210512,
-0.3363309,
0.1008179,
-0.4311774,
-0.03246222,
-0.03270159,
-0.3021439,
-0.1929839,
-0.02189694,
0.3056176,
-0.05459742,
-0.1646238,
-0.3770979,
-0.546545,
-0.3153076,
0.08279802,
0.02359999,
0.06859069,
0.406426,
0.1449646,
-0.2775372... |
[Painless ST elevation with syncope. Diagnosis by continuous electrocardiography]. | The aetiology of syncope in a 53 year old man was discovered on continuous ambulatory electrocardiography. A ventricular arrhythmia associated with ST elevation was recorded. It proved resistant to medical therapy and a double aorto-coronary bypass graft was performed. Post-operative Holter monitoring showed surgery to have been effective. | 116,620 | [
0.0691765,
-0.1196118,
-0.2543989,
-0.4197184,
0.0361847,
-0.3867859,
-0.2867619,
-0.2631349,
-0.004303393,
-0.3060184,
0.2598396,
0.3532816,
-0.01044701,
-0.3714404,
-0.285119,
-0.3028848,
-0.6342604,
0.2524749,
0.1043349,
0.247247,
0.1718627,
-0.1397022,
-0.1267252,
0... |
Multiplicity of induction patterns of rat liver microsomal mono-oxygenases and other polypeptides produced by administration of various xenobiotics. | Induction of hepatic microsomal mono-oxygenase species after administration of various xenobiotics is a well-documented phenomenon. To examine the number and specific species of rat liver microsomal membrane polypeptides involved in such responses, we have used sodium dodecyl sulphate/polyacrylamide-gel electrophoresis to analyse microsomal fractions from animals treated with a number of important xenobiotics. The following are the principal points to have emerged from this study. 1. A minimum of twelve electrophoretically distinct patterns of induction of haemopolypeptides and other polypeptides could be distinguished after administration, either singly or in certain combinations, of phenobarbital, 3-methylcholanthrene, polychlorinated biphenyls, 2-acetylaminofluorene, safrole (or isosafrole), pregnenolone-16 alpha-carbonitrile and ethanol. The patterns consisted of various permutations of the amounts of eight polypeptides of 47000-56000 mol.wt., of which at least three were haemopolypeptides. The possible identities of these polypeptides, which included species of cytochrome P-450, cytochrome P-448 and epoxide hydratase, are discussed. 2. Agents (3-methylcholanthrene, benzo[a]-pyrene, polychlorinated biphenyls, 2,3,7,8-tetrachlorodibenzo-p-dioxin and beta-naphthoflavone) that result in the induction of cytochrome P-448 caused a marked increase in two polypeptides of 54000 and 56000 mol.wt., whereas safrole and isosafrole induced only the former polypeptide. 3. Administration of 2-acetylaminofluorene resulted in the induction of two polypeptides; evidence is presented that suggests that one of these is a species of epoxide hydratase [cf. Levin, Lu, Thomas, Ryan, Kizer & Griffin (1978) Proc. Natl. Acad. Sci. U.S.A. 75, 3240-3243] ANd that the other may be a novel haemopolypeptide. 4. The overall results emphasize the complexity of the responses exhibited by rat liver microsomal fractions to the administration of xenobiotics. | 116,655 | [
-0.130071,
0.02215161,
-0.03256714,
0.1052162,
0.1856789,
-0.3936987,
-0.2248823,
0.2723306,
0.2667425,
0.177034,
-0.05224146,
-0.06445968,
0.308186,
0.01244742,
0.04121538,
-0.04331899,
-0.4473496,
-0.05813136,
0.3851704,
-0.01346875,
0.02191717,
0.2370287,
-0.3225664,
... |
Inhibition of mammary carcinogenesis of dimethylbenzanthracene treated rats with a brominated triphenylethylene. | Daily administration of a brominated triphenylethylene (TBP), to rats which received 20 mg of DMBA p. o. inhibits mammary carcinogenesis. The effect appears even with very small doses (i ppm in the diet) and seems to be dose related. With one exception (an adenofibroma) the tumours in control and treated animals were malignant. Administration of TBP prevents the appearance of corporea lutea in the ovaries but not the usual necrosis of the adrenal cortex. | 116,693 | [
-0.2178333,
0.135691,
0.08443687,
-0.05576434,
-0.3344969,
-0.06657948,
0.2584131,
-0.2150261,
-0.1166235,
0.138947,
0.06961226,
0.2215611,
0.2750505,
0.2429189,
-0.3999687,
-0.05766529,
-0.3622282,
-0.1467485,
0.3229605,
0.2377217,
0.200575,
0.2261877,
-0.09146294,
0.0... |
Altered arachidonate metabolism by platelets in patients with myeloproliferative disorders. | Platelet lipoxygenase and cyclo-oxygenase pathways were investigated by the incubation of 1(-14) C-arachidonic acid with washed platelets in 33 patients with myeloproliferative disorders, including 14 patients with chronic myeloid leukemia (CML), 12 with polycythemia vera (PV), 4 with essential thrombocythemia (ET), and 3 with myelofibrosis (MF). In patients with MF and CML, mean activities of the lipoxygenase pathway were significantly lower when compared with normal controls (p less than 0.001 and p less than 0.01, respectively). When a normal range of the activity was defined as mean +/- 2 SD, all patients with MF, 8 with CML, 6 with PV, and 1 with ET showed decreased lipoxygenase activities, while activities of the cyclo-oxygenase pathway were decreased in one of each patient with CML, PV, and ET. In 4 of 10 patients with a selective lipoxygenase deficiency, platelets were aggregated by lower concentrations of arachidonic acid than those necessary to induce normal platelet aggregation. It is suggested that the lipoxygenase activity could modulate platelet functions through its effect on arachidonate metabolism by the cyclo-oxygenase pathway and that a selective lipoxygenase deficiency could offer a mechanism for hyperfunction of the platelet, which may lead to a thrombotic tendency, one of the common features of myeloproliferative disorders. | 116,695 | [
-0.3490563,
0.08805297,
-0.1385558,
-0.3461863,
0.3114305,
-0.1288307,
0.01017138,
0.06678478,
-0.1393607,
-0.1203414,
0.04487706,
0.3836537,
-0.2597454,
0.07780975,
-0.3795939,
-0.1919539,
-0.1486987,
0.04295827,
-0.05103943,
0.4274626,
0.201712,
0.4085383,
-0.07742868,
... |
Vasoactive material present in the glomerula caudalia of the Vervet monkey. | Complex encapsulated neurovascular structures, glomus organs, occur in superficial and deep positions in many species. No direct evidence for their function exists. In skin they may serve thermoregulation by the reason for their structural complexity remains obscure. The concept of a local humoral circulatory control mechanism postulated on the basis of a very high acetylcholine content reported for human glomera coccygia has been investigated in homologous caudal glomus organs in monkey. No acetylcholine was detected. The organs contained 3-4 times the concentration of catecholamine (mainly noradrenaline) found in nearby vessels. Neither structure nor content of vasoactive component gives any support for an endocrine function. | 116,696 | [
0.02738118,
0.2950547,
-0.5262913,
-0.2013637,
0.1781539,
-0.1987905,
-0.2721446,
-0.01549614,
0.3338782,
0.2151737,
0.06139506,
-0.1199216,
0.2045596,
-0.2784825,
-0.7446122,
-0.09472601,
-0.4323539,
0.03751362,
0.2505355,
0.3210424,
0.6259861,
0.3465569,
-0.01238844,
... |
Plasticity in speech organization following commissurotomy. | For three-and-a-half years we have been studying the cognitive and conscious mechanisms in a remarkable 18-year-old man: Case P.S. This unique individual had his corpus callosum divided in order to control intractable epilepsy. Although for some time after the operation he appeared like other split-brain patients, unable to describe verbally stimuli directed to his mute right hemisphere, he behaved as if he was capable of comprehending a wide range of language-related stimuli directed to that hemisphere. Spelling by choosing the appropriate letters with his left hand, he could process nouns, verbs, rhymes, antonyms, and superordinate concepts. When asked about tachistoscopic presentations delivered to his left visual field, he either said he had seen nothing, or only a flash of light. He was also unable to identify verbally tactile 'sterognostic' inputs to his left hand. In the last year P.S. has begun to speak about stimuli directed to his right hemisphere. This series of experiments suggests that this speech is not interhemispheric transfer within the visual modality. Further, plotting the relative increased proficiency of verbal description of inputs directed to the right hemisphere, this speech system seems to be in a process of continuing development. | 116,711 | [
-0.08603686,
-0.1288844,
-0.09346303,
-0.3076795,
0.1746495,
-0.3899322,
-0.1691209,
-0.2451796,
0.2687832,
0.403951,
0.05941597,
0.1929952,
-0.2765807,
-0.3605017,
0.1113403,
0.01387013,
-0.2689369,
-0.06296346,
-0.1356681,
-0.06416298,
0.1696327,
0.4640688,
-0.1520034,
... |
Vaterite, a constituent of the eggshells of the nonparasitic cuckoos, Guira guira and Crotophagi ani. | The crystalline spherules occurring in patches on the outer surface of the eggshells of Guira guira and the bloom on the entire outer surface of those of Crotophagi ani were shown by X-ray diffraction analysis to be vaterite. | 116,746 | [
0.1113263,
0.286896,
0.02281949,
-0.2507586,
0.02720268,
-0.005803343,
-0.459872,
-0.458006,
0.3143149,
-0.1939039,
0.2031594,
0.3343614,
-0.5205563,
-0.1411105,
-0.2184308,
0.1358227,
-0.2961966,
0.3096819,
0.01611239,
-0.2325482,
0.3365008,
0.5326615,
-0.1736534,
0.29... |
Infrared absorption bands from NCO- and NCN2- in heated carbonate-containing apatites prepared in the presence of NH4+ ions. | Infrared bands at about 2200 and 2010 cm-1 in the spectra of heated synthetic and biological CO3Aps are assigned to cyanate and cyanamide ions respectively. The formation of these ions is associated with the presence of nitrogenous species in the unheated materials. | 116,758 | [
-0.2200961,
0.117027,
-0.1032551,
0.09832131,
-0.317274,
0.06971214,
-0.4798476,
-0.003238342,
0.3294535,
-0.1014421,
0.08878261,
-0.04662972,
0.05671285,
-0.3701639,
-0.6109605,
-0.3558233,
-0.5117537,
-0.01206779,
0.01603737,
0.2024991,
0.2595405,
0.09690362,
-0.1887079... |
Fine structure of isolated fibrils from the cuticle of Lumbricus sp. | A method has been developed to isolate and purify cuticular fibrils of Lumbricus. Polarizing microscopy confirms the collagenous nature of the isolated fibrils. Study in the electron microscope of isolated fibrils, negatively or positively stained, shows that they are cylindrical, unbranching and without periodic structure. Enzymatic treatment of cuticles with alpha-amylase and trypsin results in a more or less complete dissociation of the fibrils which appear clearly to be made up of helically wound bundles of filaments (30-40 A). The biophysical data and compared to the ultrastructural organization of other periodically cross-banded fibrils. | 116,769 | [
0.08150014,
0.01892712,
-0.397018,
0.01960881,
-0.2661431,
-0.3787537,
-0.2573009,
0.02353163,
0.3402857,
-0.04132858,
-0.09280556,
-0.4061708,
0.0351725,
-0.101136,
-0.3098729,
0.08470059,
-0.2695568,
0.03134681,
-0.2692344,
0.2258274,
0.318314,
0.2018285,
-0.2360195,
... |
MICC cytotoxic effector function of human T lymphocyte subpopulations bearing Fc-receptors for IgG and IgM. | Purified subpopulations of human T lymphocytes bearing Fc-IgG and Fc-IgM receptors were studied for their ability to mediate mitogen (PHA) induced cellular cytotoxicity (MICC) to chicken erythrocyte (CRBC) and DBA/Mastocytoma P815Y tumour cell targets. There were marked differences in the ability of the Fc-IgG receptor-bearing T cell (T gamma) and the Fc-IgM (T mu) to mediating MICC to CRBC and P815Y target cells. T gamma cells were very efficient killers of CRBC and T mu cells had no cytotoxic activity to CRBC. On the other hand, both the T gamma and T mu subpopulations were able to mediate MICC to P815Y tumour cell targets. | 116,786 | [
-0.04621653,
-0.278605,
0.04769697,
-0.5161974,
0.06798031,
-0.04013563,
-0.1283209,
0.4479495,
-0.1843357,
0.139283,
0.1547686,
0.425688,
-0.08068471,
-0.2320575,
-0.2955748,
-0.2801283,
-0.5550749,
-0.03555709,
-0.0004732041,
0.2933598,
0.3796588,
0.1693547,
0.04050585,... |
Structure and properties of the putrescine carbamoyltransferase of Streptococcus faecalis. | Ornithine and putrescine carbamoyltransferases from Streptococcus faecalis ATCC11700 have been purified and their structural properties compared. The molecular weight of native ornithine carbamoyltransferase, measured by molecular sieving, is 250 000. It is composed of six apparently identical subunits with a molecular weight of 39 000, as determined by cross-linking with the bifunctional reagent glutaraldehyde followed by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Using the same method, putrescine carbamoyltransferase is a trimer of 140 000 consisting of three identical subunits with a molecular weight of 40 000. Ornithine carbamoyltransferase displays a narrow specificity towards its substrate, ornithine. In contrast, putrescine carbamoyltransferase carbamoylates ornithine and several diamines (diaminopropane, diaminohexane, spermine, spermidine, cadaverine) in addition to its preferred substrate, putrescine, but with a considerable lower efficiency than for putrescine. The kinetic mechanism of putrescine carbamoyltransferase has been investigated. Initial velocity studies yield intersecting plots using either putrescine or ornithine as substrate, indicating a sequential mechanism. The patterns of protection of the enzyme by the reactants during heat inactivation as well as the results of product and dead-end inhibition studies provide evidence for a random addition of the substrates. The putrescine inhibition that is induced by phosphate does, however, suggest that a preferred pathway exists in which carbamoylphosphate is the leading substrate. The different kinetic constants have been established. The properties of putrescine carbamoyltransferase are compared to the known properties of other carbamoyltransferases. The evolutionary implications of this comparison are discussed. | 116,850 | [
0.04191573,
0.2220464,
0.168955,
-0.07929779,
0.2339547,
-0.2828751,
0.193582,
0.2365554,
0.2370489,
-0.05404682,
0.07413502,
0.04923838,
-0.1287424,
0.1589068,
-0.09922796,
0.3228975,
-0.4189307,
-0.03740105,
-0.1272738,
0.2094097,
0.2271668,
0.3004936,
-0.2399955,
0.3... |
Craniofacial growth in olive baboons (Papio cynocephalus anubis): browridge formation. | The examination of a developmental series of skulls of olive baboons has led to the generation of a model for browridge formation. Evidence in support of the model comes from surface configuration of browridge bone as studied by scanning electron microscopy, histology of the bone by the analysis of thin sections and by measurements that reflect both depositional cycles in browridge bone and the biomechanics of mastication. The model relates sudden changes in the biomechanical system caused by the tooth eruption cycle to cyclical changes in the formation of browridge bone. We suggest that browridge bone is added during periods of peak stress caused by the positive response of the masticatory muscles to relatively sudden reduction of their biomechanical efficiency. | 116,911 | [
0.3689413,
0.103241,
-0.3365642,
0.1722327,
0.1359642,
-0.2841978,
-0.2534321,
-0.4632122,
0.1617792,
-0.2374352,
-0.1940748,
-0.4413287,
-0.4063266,
-0.3590589,
-0.5428401,
-0.1111238,
-0.203824,
0.179749,
0.1761771,
-0.03908553,
0.2605377,
0.06374015,
-0.1474135,
-0.0... |
Control of glycogen metabolism in the developing turkey poult. | An experiment was conducted with young turkey poults to evaluate factors controlling glycogen metabolism in the period following hatching. Glucose and sucrose solutions were given along with a standard starter diet. Liver and carcass glycogen were measured on days 1, 4 and 6. Liver glycogen synthetase (EC 2.4.1.21) and phosphorylase (EC 2.4.1.1) were also assayed at these times. The characteristics of active and inactive glycogen synthetase at these times were determined and sensitivity of the active and inactive forms were related to physiological concentrations of glucose-6-phosphate. Supplemental glucose or sucrose increased carcass glycogen in comparison to controls; however, but sucrose was more effective than glucose in promoting liver glycogen synthesis in 4- and 6-day-old poults. There was an age dependent increase in carcass glycogen between days 1 and 6, but a decrease in liver glycogen between days 4 and 6. The activation of liver glycogen synthetase is incomplete in 1 day old poults but activity increases during the 1st week of life. Activation of glycogen synthetase decreased the apparent Ka for glucose-6-phosphate. Phosphorylase inactivation in vitro was not affected by age. Liver glucose-6-phosphate increases rapidly after hatching and the concentration is related to the in vitro Ka derived for both active and inactive synthetases. Both glucose and sucrose increased liver glucose-6-phosphate at days 4 and 6 as well as glycogen synthetase activity. The increase in enzyme activity may be caused indirectly by an allosteric effect of glucose-6-phosphate. Phosphorylase, while not affected by supplemental carbohydrates, did decrease in activity between days 4 and 6. The decrease in activity could affect the phosphorylase a/ synthetase a ratio and change glycogen metabolism. | 116,912 | [
0.06892602,
0.08974311,
-0.4615717,
-0.1074861,
0.125234,
-0.3216411,
0.1216749,
0.04878501,
-0.1697547,
-0.3396951,
0.1204533,
0.2569236,
-0.1841224,
0.01633138,
-0.3505191,
0.1482149,
-0.7667302,
0.09807362,
0.01401247,
0.2166016,
0.4432704,
0.4015625,
-0.1245459,
-0.... |
Female aggression and male membership in troops of Japanese macaques and olive baboons. | A detailed comparison of Macaca fuscata and Papio anubis reveals that female aggression to males has an effect on male association with females in both species, but only in M. fuscata do females appear to be able to limit the number of males in their troop. It is suggested that this is the reasons why 'solitary' males are commonly reported in M. fuscata, but rare in P. anubis, and may be related ultimately to the different degrees of sexual dimorphism in the two species. | 116,946 | [
-0.06859519,
0.2829455,
0.01053165,
-0.4115563,
-0.00995229,
-0.4668537,
-0.2527853,
-0.28472,
-0.000504547,
-0.180531,
0.1518418,
-0.0644338,
0.1413694,
-0.1109384,
-0.2533531,
-0.6428735,
-0.2902556,
0.1971683,
0.1544644,
-0.2587227,
0.0002740817,
-0.03980091,
-0.191422... |
Enzyme immunoassay for detecting Brucella antibodies in cow's milk. | An enzyme immunoassay (EIA) was developed for detecting Brucella antibodies in milk of cows infected with Brucella abortus. The enzyme immunoassay using heat-killed cells of B. abortus strain 19 was of comparable sensitivity to the Brucella ring test in detecting antibodies in milk of a reference positive control cow experimentally infected with B. abortus strain 2308 and in milk of 16 naturally infected cows from which B. abortus was isolated. No detectable enzyme immunoassay reactions were present in milk of 11 noninfected controls. The enzyme immunoassay is a procedure which can be readily automated so that screening tests for brucellosis could be conducted at a reference laboratory where uniform conditions can be maintained. | 117,023 | [
-0.07280783,
-0.01583964,
0.2593964,
0.2086811,
-0.2005062,
0.07971496,
-0.001386205,
0.1744309,
0.1601047,
-0.1216839,
0.2924846,
0.2068722,
0.009061232,
0.1603771,
-0.2459122,
-0.1094044,
-0.882678,
0.3910521,
-0.3818073,
-0.02459507,
0.4562107,
0.2818689,
-0.0597859,
... |
Use of 2-mercaptoethanol to facilitate detection and classification of IgM abnormalities by immunoelectrophoresis. | IgM immunoglobulins in elevated serum concentrations have a tendency to polymerize and form aggregates. When subjected to immunoelectrophoreses these proteins may deposit at the point of origin. This can result in failure to detect an IgM abnormality or the masking of other serum protein abnormalities migrating near the area of application. This paper demonstrates the importance of using reducing agents such as 2-mercaptoethanol not only to detect and/or to confirm the monoclonal IgM gammopathies but also to unmask other protein abnormalities. | 117,053 | [
-0.09716001,
0.1212526,
0.06905612,
-0.3770605,
0.08225621,
-0.0994847,
-0.08512563,
0.1456868,
0.226269,
0.1453444,
0.1188098,
0.1253636,
0.2848747,
0.2235518,
-0.1682332,
0.09373837,
-0.363817,
0.01609476,
-0.02129739,
0.235789,
0.1633029,
-0.0938596,
-0.03683435,
0.0... |
The effects of UVB and 7, 12, dimethylbenz(alpha)anthracene (DMBA) on epidermal melanocytes of the tail in C57BL mice. | The morphological and numerical changes in the epidermal melanocyte system of the tail of C57BL mice were studied after exposure to 7, 12 dimethylbenz(alpha)anthracene (DMBA) followed by UVB irradiation. The dorsal aspect of the tail was exposed for 5 days per week, for a total duration of 10 weeks, to a daily dosage of 0.1 J/cm2 UVB (peak 310 nm). Once a week, 0.2 ml of 0.15% DMBA in acetone was locally applied to the irradiated areas. Biopsies were studied by the combined skin-splitting DOPA and electron microscopic techniques. After 10 weeks of DMBA treatment the following changes were observed: DMBA treatment the following changes were observed: the original brick-like arrangement of melanocytes became confluent, melanocytes were irregularly shaped, dendrites shortened and clumped together, and the outer root sheaths of the hair follicles became covered with melanocytes. There was a significant increase in the number of DOPA positive melanocytes at the end of the first week in DMBA and DMBA + UVB treated skin. Ultrastructurally, an increase in melanosome formation in melanocytes and transfer into keratinocytes was found, as well as redistribution of melanosomes from singlets and doublets into larger groups. Damage of melanocytes by the DMBA treatment was seen, but no inflammation or tumor formation was observed. | 117,059 | [
0.08704218,
-0.1556927,
-0.2372964,
0.07400436,
0.3606817,
-0.567608,
0.1225486,
0.1257718,
0.171359,
0.1269005,
-0.1494772,
-0.1205015,
0.1384121,
0.3097602,
-0.1980204,
0.1982932,
-0.1336781,
0.09220112,
-0.07791181,
0.037683,
0.4534452,
0.1698472,
0.03278701,
-0.1593... |
Ovarian activity, and total gonadotrophin and gonadotrophin releasing hormone levels in response to some synthetic non-steroidal ovulating agents in the catfish, Heteropneustes fossilis (Bloch). | Effects of clomiphene citrate, cyclofenil and prostaglandins (PGE1 and PGF2 alpha) on ovarian 32P uptake, on gonadotrophin levels in the pituitary gland and blood serum and on a gonadotrophin releasing hormone-like (GnRH-like) substance in the hypothalamus were investigated in Heteropneustes fossilis. These drugs were very effective in increasing the serum level of gonadotrophin with a subsequent increase in ovarian 32P uptake in sham-hypophysectomized recipients. All the drugs except cyclofenil failed to stimulate 32P incorporation by the ovary in hypophysectomized fish. Clomiphene citrate and cyclofenil also induced a significant increase in the GnRH-like factor in the hypothalamus of H. fossilis. Such a response was not obtained in fish treated with PGE1 and PGF2 alpha. It seems likely that the action of clomiphene is routed through the hypothalamo-pituitary-ovarian axis and that of prostaglandins directly through the pituitary-ovarian axis. The action of cyclofenil is bimodal; one effect like that of clomiphene and the other direct upon the ovary probably by increasing its sensitivity to the available gonadotrophin. | 117,067 | [
-0.1500198,
-0.04532913,
0.07467907,
-0.03729971,
-0.07689986,
-0.2883449,
-0.2481527,
0.0612517,
-0.07616295,
0.02747933,
-0.02705053,
0.3062139,
-0.07636131,
-0.01252816,
-0.2184308,
-0.2845815,
-0.4838318,
-0.0347871,
0.1901417,
0.4436549,
0.08752926,
0.3024239,
-0.363... |
Specific Lyt 123 cells are involved in protection against Listeria monocytogenes and in delayed-type hypersensitivity to listerial antigens. | Specific anti-Lyt antisera and complement were used to determine the Lyt phenotype of peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice. It was found that Lyt 123+ T cells are crucially involved both in protection against listerial infection and in delayed-type hypersensitivity (DTH) to listerial antigens. Thus, both functions critically depend on a T-cell subclass phenotypically different from that which mediates DTH to noninfectious antigens and help in antibody formation on the one hand, as well as those T cells mediating cytotoxic reactions on the other. | 117,073 | [
0.03070595,
-0.3304705,
-0.1265705,
-0.2043612,
0.01470396,
-0.193896,
0.02051735,
0.03528537,
0.1184139,
0.1240204,
0.03102696,
0.3351517,
-0.06841121,
0.1680542,
-0.4713669,
-0.2937461,
-0.5175247,
0.1202365,
-0.2260802,
0.2773086,
0.3374227,
0.2076335,
-0.07580356,
-... |
The intracellular survival and growth of gonococci in human phagocytes. | In reassessment of previous tests for intracellular survival, results have been confirmed and additional evidence obtained indicating that some gonococci can survive and multiply in human phagocytes. Use was made of the ability of penicillin to penetrate phagocytes and to kill only actively growing organisms. In microscopic counts on 33 urethral exudate smears, an average of 49% of gonococci were associated with polymorphonuclear phagocytes. The organisms were unevenly distributed amongst the phagocytes, with most cells uninfected and some containing large numbers. Many phagocytes also remained uninfected in tests in vitro with low gonococcal inocula although experiments with large inocula showed that most phagocytes could ingest gonococci. It is proposed that ingestion of one gonococcus may stimulate the phagocytes to take up more. Phagocytes were killed and disintegrated after ingesting large numbers of gonococci and similar effect in vivo may be responsible for the large clumps of organisms seen in urethral exudate. These results underline the probable importance in the pathogenesis of gonorrhoea of intracellular survival in phagocytes. | 117,078 | [
0.03972051,
-0.2734249,
-0.4055465,
-0.09891811,
0.05290515,
-0.2652435,
-0.1870588,
-0.1624782,
-0.1045509,
-0.3945664,
-0.00120343,
0.1202244,
-0.06293264,
0.5874684,
-0.3603957,
-0.1090329,
-0.3505086,
0.02779988,
-0.07175153,
0.1631893,
0.3632001,
0.4352669,
0.2452308... |
Complete lysis of glutamic acid-producing bacteria by the use of antibiotics which inhibit the biosynthesis of cell walls. | A novel method for obtaining complete lysis of coryneform bacteria has been devised. Bacteria in the late-exponential phase of growth are treated with antibiotics which inhibit biosynthesis of the cell wall. Organisms treated in this way show increased sensitivity to lysis by added lytic enzymes. The preparation of DNA from this group of organisms is thus greatly facilitated. | 117,079 | [
-0.05788759,
0.01670909,
-0.1696201,
-0.01298304,
0.05923599,
0.01505526,
-0.05844684,
0.07746411,
0.2923836,
-0.0605908,
-0.1105031,
0.4690632,
-0.09983399,
0.1901023,
-0.4241066,
0.1629244,
-0.6117775,
0.0561658,
-0.09195399,
0.218491,
0.6578768,
0.1982376,
-0.1470406,
... |
Bullet injury to the pituitary gland: a rare cause of panhypopituitarism. | An unusual case of head injury with a direct bullet injury to the pituitary gland is described. The hormonal profile one month after the injury showed severe panhypopituitatism which did not improve one month after surgical removal of the intrasellar bullet fragment. | 117,082 | [
-0.2704899,
0.0229186,
-0.1139506,
-0.2084429,
-0.2099656,
-0.1799581,
-0.279369,
0.01631192,
0.1030944,
0.3652609,
0.191641,
-0.09797161,
-0.2018484,
-0.4787284,
0.1710952,
-0.1700139,
-0.1145468,
0.1634928,
0.3010991,
-0.1324262,
0.233989,
0.006083585,
0.003329564,
-0... |
Possible mechanism of the inhibitory effect of ouabain on renin secretion from rat renal cortical slices. | 1. The effects of ouabain on renin secretion by rat renal cortical slices were studied. 2. Renin secretion was inhibited by 10(-3) M-ouabain in the presence of free Ca (10(-4) to 2.6 x 10(-3) M). Inhibition was blocked at Ca less than 10(-8) M. 3. The effect of free Ca on ouabain-inhibition was shown to be independent of the presence of EGTA, completely reversible, and unrelated to passive leakage of renin from non-viable cells, as assessed by simultaneous release of lactate dehydrogenase activity (LDH). 4. It is proposed that, as a result of inhibition of Na, K-ATPase by ouabain, (a) intracellular Na increases in the renin-secreting juxtaglomerular cells, (b) intracellular Ca increases, via an Na-Ca exchange mechanism, and (c) that Ca accumulation, in some unknown manner, inhibits renin secretion. | 117,099 | [
-0.2257794,
0.119906,
-0.2694398,
0.101253,
0.1185915,
-0.1616641,
0.08768101,
-0.01147367,
0.1190254,
0.07024346,
0.02090902,
0.2935003,
0.09372203,
0.1110279,
-0.8110602,
0.1612178,
-0.4883691,
0.2392372,
-0.09744281,
0.07138168,
0.3383427,
-0.04378821,
0.05959639,
0.... |
3-[(1,2,3-Thiadiazol-5-ylthio)methyl]cephalosporins. | The syntheses of ten 3-](1,2,3-thiadiazol-5-ylthio)methyl]cephalosporins, made by displacement of the 3'-acetoxy group by the novel thiol derivatives, potassium 1,2,3-thiadiazole-5-thiolate and dipotassium 1,2,3-thiadiazole-4-carboxylate-5-thiolate, are described. Several of the compounds showed good in vitro antibacterial activity against both Gram-positive and Gram-negative organisms. The subcutaneous in vivo activities against Staphylococcus aureus were generally less than that of cafazolin. Four of the compounds were administered orally and all were active; the 7 beta-(thiophen-2-acetamido) and 7 beta-(D-2-amino-2-phenylacetamido)-3-[(1,2,3-thiadiazol-5-ylthio)methyl] compounds were equally active by either route, with a PD50 of ca. 1 mg/kg. | 117,106 | [
-0.1832123,
-0.03287368,
-0.3201761,
-0.02239521,
-0.1707874,
0.1807341,
-0.2991279,
-0.02013521,
-0.05851832,
-0.2768854,
0.3051041,
0.2279278,
0.2704017,
0.2172501,
-0.6660408,
-0.3487148,
-0.4785508,
0.01342622,
-0.1701349,
0.2811489,
0.00006134688,
0.1106236,
0.132400... |
A new estimate of sequence divergence of mitochondrial DNA using restriction endonuclease mappings. | A new estimate of the sequence divergence of mitochondrial DNA in related species using restriction enzyme maps is constructed. The estimate is derived assuming a simple Posisson-like model for the evolutionary process and is chosen to maximize an expression which is a reasonable approximation to the true likelihood of the restriction map data. Using this estimate, four sets of mitochondrial DNA data are analyzed and discussed. | 117,114 | [
-0.0836357,
-0.2335007,
-0.3055205,
0.1601599,
0.1585117,
-0.1578374,
-0.2980971,
0.03133493,
0.2289646,
0.01218202,
-0.2642304,
0.03455904,
0.2875462,
0.2621255,
-0.4133014,
0.1759593,
-0.4526192,
0.05518321,
0.1560587,
0.01451887,
0.2935984,
-0.1090994,
-0.194313,
-0.... |
Peripelvic new bone formation following straddle injuries in hemophiliac patients: report of two cases. | Of 123 patients suffering from either Haemophilia A or B examined at our clinic two were found with peripelvic new bone formation following straddle injuries. An awareness of the possibility of peripelvic ossification should prompt immediate treatment to stop hemorrhage into soft tissues in such cases. | 117,117 | [
-0.2188591,
0.03585938,
-0.03341816,
-0.1555686,
-0.0999575,
-0.2001108,
-0.3649124,
0.05808475,
-0.09990036,
0.01111683,
0.1834861,
0.1795822,
-0.1961545,
-0.2170135,
-0.2349338,
-0.08425379,
-0.3852717,
-0.1133935,
-0.07230255,
-0.0697538,
0.2646145,
0.2339692,
-0.08217... |
Studies of the toxicity of an intravenous fat emulsion. i. Hematologic changes and survival after administration of a soybean oil (FE-S15) in beagles. | The potential toxicity of FE-S15 (B. Braun-Melsungen), a soybean-oil fat emulsion used in parenteral nutrition, was studied in dogs. Forty pure-bred beagles, in two experimental groups (FE-S15 at 9 and 4 g/kg/day) and two corresponding control groups (receiving Dextrose-Ringer's solution), were given daily infusions for 28 days via a central venous catheter. Vital signs and hematologic, biochemical, and bacteriologic changes were monitored closely. When compared with control groups, no significant weight loss was observed in either group; the food intake decreased only in animals receiving fat in high doses. Hemoglobin and hematocrit decreased in all groups during infusion, the greatest fall observed in the group receiving high-dose fat infusion where the hematocrit declined from 45.5% to 31.7%. This decrease was significantly different from the controls only during one observation period. Clinical signs, such as lethargy, vomiting, diarrhea, loss of appetite and fever were observed infrequently in both experimental and control animals, more often in those treated with high-dose fat infusion. It appears that the fat emulsion FE-S15 causes only minor side effects but otherwise is well tolerated in dogs at a potentially toxic level. | 117,123 | [
-0.1412087,
0.1035987,
-0.3563268,
-0.4915595,
0.02336616,
-0.4358342,
0.1346878,
-0.1176127,
-0.2970071,
-0.2037752,
0.01772329,
0.03179072,
-0.08809766,
-0.2141261,
-0.319405,
-0.3733867,
0.4214364,
0.2514733,
0.0370867,
0.6723393,
-0.1260052,
0.3304181,
-0.04914583,
... |
Spontaneous zinc deficiency in marmosets, saguinus mystax. | Moustached marmosets, Saguinus mystax, developed alopecia, skin lesions and general debilitation approximately 6 months after being brought into our laboratory. These lesions resembled the signs of zinc deficiency in pigs, rats and squirrel monkeys. The marmosets were fed a commercial monkey diet containing 150 parts per million zinc and about 100 g of apples and oranges per week. The addition of 40 parts per million zinc to the drinking water brought about improvement in hair coat and skin condition. | 117,220 | [
0.07699385,
-0.01788233,
-0.1654295,
-0.1839925,
0.0284115,
0.03496295,
-0.3047284,
-0.1737222,
0.1377135,
-0.3238022,
0.2954124,
0.07015797,
0.02727902,
0.2262606,
-0.5696058,
0.2390085,
-0.4161164,
0.0764381,
-0.1692533,
-0.2811137,
0.1217686,
0.07283058,
-0.07121859,
... |
[Urinary tract infections. Therapeutic failures and course monitoring (author's transl)]. | The proportion of reinfections and relapses in urinary tract infections amounts to about 35-70%. Hydrokinetic conditions (e.g. size of bladder, frequency of micturition, rate of multiplication of the pathogens, adhesiveness of bacteria) not seldom lead to a discrepancy between the in vivo response of the pathogen to the chemotherapeutic agent and the corresponding MIC determined in vitro. Ten causes for the therapeutic failure are discussed in greater detail. Monitoring the course in good time with due regard to the risks is essential. A scheme for microbiological monitoring investigations before beginning therapy, during treatment and after discontinuing the medicament, as well as for long term therapy is suggested. | 117,322 | [
0.01208916,
-0.12857,
-0.1877888,
-0.2427967,
0.1957368,
-0.2719969,
-0.2516413,
0.1124712,
0.1075643,
-0.1384961,
0.235005,
0.1670152,
0.1429439,
0.2381307,
-0.608758,
-0.01138398,
-0.1048215,
0.2440757,
-0.1693022,
0.09406434,
0.4923206,
-0.06139183,
-0.03886839,
-0.0... |
[Diagnostic significance of cholinesterase determination in human serum (author's transl)]. | The differential diagnostic importance of CHE determination in searching for and observing the course of liver diseases is emphasized in the literature within the small and large enzyme pattern (eg. Gergs 1976). In chronic hepatitis and cirrhosis normal or almost normal values suggest a favorable prognosis. Greatly reduced CHE points to limitation of liver function and a severe form of the disease suggesting toxic influences and considerable deficiency. Rapidly falling CHE activity both in acute hepatitis and in chronic inflammations of the liver point to the danger of threatening hepatic coma. | 117,340 | [
-0.0166539,
-0.02939652,
-0.1472786,
-0.297124,
-0.02619188,
-0.1025946,
-0.1636819,
0.03765941,
0.02889985,
-0.02939829,
-0.06841925,
0.1078205,
0.1159739,
0.3826271,
-0.5924569,
0.00466753,
-0.2747264,
0.2558714,
0.02380722,
0.4836695,
-0.06086429,
0.07421574,
0.0010973... |
Mutagenic, cancerogenic and teratogenic effects of alcohol. | Alcohol is mutagenic, cancerogenic and teratogenic in man. Ethanol is mutagenic via its first metabolite, acetaldehyde. This is substantiated by the findings that acetaldehyde induces chromosomal aberrations, sister-chromatid exchanges and cross-links between DNA strands. Methanol, a contaminant of many alcoholic beverages, is also mutagenic via its metabolite, formaldehyde. In addition, different indirect pathways may lead to mutations by alcohol. The cancerogenic activity of alcohol remains unverified by modern standard carcinogenicity tests. Ethanol and other alcohols, as well as aldehydes, inhibit RNA synthesis in cells and in cell-free transcriptional systems. A reduction of cellular RNA synthesis may play an important role in the mutagenic, carcinogenic and teratogenic activity of alcohol. | 117,354 | [
-0.2907233,
0.07876493,
-0.1382035,
-0.06446587,
0.2954336,
0.003495046,
-0.1874233,
0.09595762,
0.193673,
0.1059006,
0.1735524,
0.06873167,
0.175758,
0.2129525,
-0.2991949,
-0.05415706,
-0.4189126,
0.01631835,
-0.1369901,
0.1461286,
0.3280938,
0.1323586,
-0.2673181,
-0... |
[Treatment of acute renal insufficiency in intensive care. Current perspectives with TPA and proxazole]. | A critical assessment is made of cases of ARF observed in a resuscitation department over the last three years. Reference is made to the physiopathological and pharmacological premises underlying the employment of a different therapeutic protocol, based on the maintenance of high diuresis with proxazol and the use of TPA to establish a nitrogen balance with a high cal/N ratio, coupled with the administration of histidine. | 117,401 | [
-0.004856994,
-0.2102315,
0.318873,
-0.006942158,
-0.004264946,
-0.0399327,
-0.1143515,
-0.3834478,
-0.07038258,
0.2249107,
-0.09089751,
-0.07072435,
-0.2156307,
0.2865207,
-0.3759061,
-0.2459377,
-0.09857132,
0.2833115,
-0.09869879,
0.3950796,
-0.01513883,
0.02586071,
0.... |
A circulating inhibitor of platelet aggregation in Bartter's syndrome. | Aggregation studies were performed on platelets from five patients with Bartter's syndrome. Epinephrine failed to induce aggregation in all five patients. Adenosine 5'-diphosphate (ADP) produced a single reversible phase of aggregation, and there was depressed sensitivity to collagen. Response to ristocetin was normal. There was a dose-related inhibition of ADP-induced platelet aggregation when plasma from the patients was addeded to normal platelet-rich plasma. This inhibition was diminished or absent when patients were receiving aspirin. Washed platelets from two patients who were no longer undergoing aspirin therapy, showed a normal response to epinephrine in normal platelet-poor plasma. Bleeding time was reduced from 23 minutes to 12 minutes in one patient while on aspirin therapy. These studies suggest that a circulating inhibitor of platelet aggregation, probably of prostaglandin origin, is present in the plasma of patients with Bartter's syndrome. | 117,424 | [
-0.2071683,
0.2969846,
-0.2882742,
-0.4751719,
0.2075612,
-0.3241486,
-0.1759889,
0.1835614,
0.2845147,
0.3569694,
0.09333967,
0.5038711,
-0.1517475,
-0.07016934,
-0.2809341,
0.05301933,
-0.1477782,
0.100844,
-0.2253822,
0.4731598,
0.2115979,
0.08948099,
-0.04259396,
0.... |
Organochlorine residues in six species of estuarine birds, South Carolina, 1971-75. | In South Carolina between 1971 and 1975, authors evaluated the occurrence of organochlorine residues in the laughing gull (Larus atricilla), white ibis (Eudocimus albus), glossy ibis (Plegadis falcinellus), American oystercatcher (Haematopus palliatus), willet (Catoptrophorus semipalmatus), and ruddy turnstone (Arenaria interpres). Tissues of birds found dead and eggs were analyzed, eggshell thicknesses were measured, and incidental observations were made of reproductive success and population status. Eggshell thickness of the white bis, American oystercatcher, and laughing gull were not significantly different (P less than 0.05) from the pre-1947 norms. DDE and polychlorinated biphenyls (PCBs) were found most frequently and at the highest concentration in eggs. DDE residues declined significantly in oystercatcher eggs, and declined slightly in laughing gull eggs; no change was noted in white ibis eggs. No consistent trends were found for dieldrin and PCBs. Authors found no obvious problems with reproductive success of any species. Populations of the five species breeding in South Carolina appear stable. The white ibis and laughing gull in South Carolina have experienced population explosions over the past 50 years; the glossy ibis has increased substantially since the first documented breeding records in 1947. | 117,426 | [
-0.06955916,
0.06623553,
-0.02868951,
-0.2373759,
-0.03533584,
-0.03531042,
0.06116256,
0.08706931,
0.2050276,
0.1744058,
0.2630072,
0.1964073,
-0.000268036,
0.1321263,
-0.1444375,
-0.2066403,
-0.5161254,
0.1827286,
0.5908307,
0.269262,
-0.1949368,
0.3811702,
-0.2104578,
... |
Residues of polychlorinated biphenyls and DDT in water and sediment of the St. Lucie Estuary, Florida, 1977. | sigma DDT residues in the St. Lucie River bottom sediments increased after Lake Okeechobee water was discharged through the St. Lucie Canal into the area. sigma DDT levels were highest in sediment samples from the Palm City area, ranging from 1.8 ppb to 6.15 ppb. Sediment samples from the A1A Highway Bridge area contained 1.6-6.8 ppb Aroclor 1254. Levels of polychlorinated biphenyls (PCBs) and sigma DDT in sediment samples from the junction of the St. Lucie and Indian Rivers were not detectable. Surface water samples from the estuary did not show any detectable DDT or PCB residues. | 117,428 | [
0.07111037,
0.0213707,
0.1597866,
-0.2355807,
0.07154152,
0.01306889,
-0.4830278,
-0.0205125,
0.1059932,
0.2326498,
0.1027779,
-0.1059537,
0.1081011,
0.2277548,
-0.01415449,
-0.1579138,
-0.5728996,
0.06366378,
0.4015631,
-0.1151341,
-0.004293656,
0.1401709,
-0.07638273,
... |
Further characterization of a cell-free system for measuring replicative and repair DNA synthesis with cultured human fibroblasts and evidence for the involvement of DNA polymerase alpha in DNA repair. | DNA repair synthesis can be specifically measured in osmotically opened, confluent cultured human fibroblasts after exposure to DNA damaging agents such that both induction and mediation of DNA repair synthesis can take place in this cell-free system. Alternatively, by utilizing osmotically shocked, log phase cells and altering the DNA precursors, pH and ionic strength, replicative DNA synthesis can be specifically monitored. Autoradiographic studies show that virtually all of the nuclei from the lysates of the confluent, UV-iradiated cells are lightly labeled in the fashion characteristic of DNA repair. By contrast, only a fraction of nuclei is labeled in a population of unperturbed, opened log phase cells and the labeling is heavy and characteristic of replicative synthesis. Furthermore, equilibrium density gradient sedimentation shows that DNA synthesis in lysates of log-phase cells is semiconservative, whereas that with UV-irradiated cells is repair synthesis. This open cell system has been used to study the enzymology of DNA repair. Thus, dideoxythymidine triphosphate, a specific inhibitor of DNA polymerases beta and gamma, does not inhibit either replicative or repair synthesis. By contrast, aphidicolin, a specific inhibitor of DNA polymerase alpha, inhibits DNA repair and replicative synthesis in both intact and permeabilized cells. Finally, phage T4 UV-exonuclease stimulates repair synthesis, but only when phage T4 UV-endonuclease is also added to the UV-irradiated nuclei. | 117,431 | [
-0.1980892,
-0.2345935,
-0.462907,
-0.1302122,
0.343526,
-0.3208154,
-0.1415793,
0.07595651,
0.3653007,
0.3797455,
0.03496443,
-0.290935,
-0.2078808,
0.1610039,
-0.5440715,
0.03600259,
-0.297547,
0.1369163,
-0.08074851,
0.4740201,
0.542423,
-0.2121101,
-0.3223882,
0.154... |
[Products of bacterial destruction of sodium dodecyl sulphate]. | The products of Na dodecyl sulphate destruction by the three bacterial cultures--Pseudomonas aeruginosa, Flavobacterium devorans, Achromobacter guttatus--were examined. The cultures were shown to decompose Na dodecyl sulphate in a similar way. The primary mechanism of destruction was found to be hydrolysis of the sulpho-ester bond in the molecule, leading to the separation of sulphate-ion and formation of dodecanol. Products of bacterial destruction of alkyl sulphates did not show foam forming capacity. | 117,448 | [
-0.0637179,
0.2378116,
0.03084761,
0.1931722,
0.1832244,
-0.2976665,
-0.2244611,
0.2229594,
0.04754505,
-0.1161626,
-0.1164062,
-0.1712117,
0.007414667,
-0.2042696,
-0.3711502,
-0.2365545,
-0.2583637,
0.2510252,
0.2388915,
0.2286357,
0.08461552,
0.2184129,
-0.2933795,
-... |
The Ranvier node as a chemo-electric pulsatory unit: a study of its structure-functions relations. | The Ranvier node of (Rana temporaria) frog nerve fibres is investigated by electron microscopy, Particular attention is given to the paranodal septate structures and to the extranodal junction of two Schwann cells. An interpretation of the functional meaning of these structures along with a quantitative analysis of the Schwann extranodal junction as regards the diffusion from/to the node is attempted. A 73 per cent reduction of the diffusion coefficient is obtained if the extranodal Schwann cell processes are considered impermeable to the diffusing vectors which indicates a protective role. Only 1 per cent reduction is obtained in the case of excitation-involved cations to which the Schwann cell membrane is considered to be permeable. This indicates the active role of the Schwann cell in the extranodal area ion diffusion, by minimizing the variations in ion concentration near the nodal membrane. Thus the nervous fibre-Schwann cell assembly may be regarded as a balanced pulsatory chemo-electric unit. | 117,470 | [
-0.02732937,
0.1826395,
-0.125181,
0.3209425,
-0.2069586,
-0.4409341,
-0.2055088,
-0.1686797,
0.1504602,
-0.03146059,
0.01469774,
-0.162016,
0.06603105,
0.03529412,
-0.1676644,
-0.1877439,
-0.3772334,
0.1956255,
-0.1075592,
-0.03892869,
0.2093371,
0.1508811,
0.1547968,
... |
Effects of trifluoperazine, chlorpromazine, and haloperidol upon temporal information processing by schizophrenic patients. | Healthy controls, unmedicated, actively symptomatic schizophrenics, and similar patients undergoing treatment with either trifluoperazine, chlorpromazine, or haloperidol were studied with tests of temporal discrimination and measures of transmitted information shown previously to be sensitive to various kinds of brain dysfunction, including haloperidol effects in a nonpsychotic population. Variations in psychophysical method, cognitive load, discrimination complexity, and sense-mode conditions permitted representative sampling of the temporal processing. Untreated, actively psychotic patients showed no impairment of temporal processing while all three antipsychotic medications were associated with significant deficit; trifluoperazine and haloperidol produced the most deficit, with chlorpromazine in the middle between the higher potency drugs on the one hand and unmedicated patients and healthy controls on the other. | 117,479 | [
-0.1957368,
0.1740331,
-0.1179261,
-0.1593456,
0.2178301,
-0.4776586,
-0.2124847,
-0.0515398,
0.06150369,
-0.096381,
0.008657157,
0.117952,
-0.2315962,
0.1503872,
-0.05692524,
-0.08833098,
-0.5815458,
0.05259554,
-0.5356766,
0.1543555,
-0.1078052,
0.3613261,
0.1291197,
... |
Decreased uptake of 5-hydroxytryptamine in blood platelets from patients with endogenous depression. | 5-Hydroxytryptamine (5-HT) uptake was studied by using blood platelets from 13 patients with endogenous depression (Hamilton rating scale 33 +/- 7) and 13 healthy volunteers. An improved method with a short incubation time and low substrate concentration was used, and the incubation was performed in Krebs-Henseleit buffer (pH 7.4) at 37 degrees C. A clear difference in 5-HT uptake by blood platelets was noted: The Vmax of the reaction in patients was 39, and in controls 71 pmol per 2 x 10(7) platelets in 5 min. There was no significant difference in the Km. After a 4-week treatment with imipramine, a competitive inhibition of 5-HT uptake with an increased Km was seen; after a similar treatment with amoxapine there was little change in 5-HT uptake. Amoxapine was inferior to imipramine as an inhibitor of 5-HT uptake, also in vitro. There was no difference in clinical recovery in these treatment groups. These results may be of importance so as to understand the potential biological differences between depressed patients and normal persons. | 117,482 | [
-0.3031557,
-0.1962194,
-0.5053934,
-0.1865189,
0.04400018,
-0.04733396,
-0.07993359,
0.1873862,
-0.3381579,
-0.2030699,
0.001975692,
0.1024078,
0.0008512291,
0.1370396,
-0.6897519,
-0.1821348,
-0.4085358,
0.1749392,
-0.1989547,
0.4448029,
0.1255014,
-0.02826949,
0.093493... |
Water intake and time course of drinking after single or repeated chlordiazepoxide injections. | Chlordiazepoxide (5.0, 10.0 or 15.0 mg/kg) was given to rats either acutely or for 9 consecutive days. Its effects were examined in a 15-min drinking test in which latency to drink, volume of water consumption and the time-course of drinking were measured. Chlordiazepoxide (10.0 mg/kg) produced the strongest stimulant effect on drinking and enhanced the frequency of occurrence of drinking both at the beginning and at the end of the test period. Chlordiazepoxide (15.0 mg/kg) delayed the onset of drinking and its peak effect was observed later than for other injection conditions. Single and repeated administration of chlordiazepoxide had the same effects on the measures taken in the test. Initial sedation to the drug treatment and subsequent tolerance to this effect were not, therefore, factors influencing the drug effects observed in this experiment. Possible mechanisms underlying the stimulation of drinking by chlordiazepoxide are considered. | 117,488 | [
-0.2118934,
-0.1716524,
-0.4916398,
-0.07310542,
0.3513378,
-0.4843599,
-0.3267617,
-0.1596191,
-0.04752206,
-0.1327657,
0.1274941,
0.4742693,
-0.05757013,
0.1359244,
0.1349601,
-0.0731003,
-0.1464097,
0.3454188,
-0.3340432,
0.3012261,
0.0547221,
-0.003975213,
0.09529268,... |
Involvement of central cholinoceptors in Metrazol-induced convulsions. | Atropine sulphate (10 mg/kg IP) afforded 90% protection against clonic convulsions induced by standard doses of metrazol (80 mg/kg SC) in mice, whereas atropine methonitrate (10 mg/kg IP) did not offer any protection. Furthermore, physostigmine (1 mg/kg IP) caused recurrence of convulsions in atropinzed metrazol-treated mice and converted the subconvulsive dose of metrazol (40 mg/kg SC) into a 100% convulsive dose. However, neostigmine (1 mg/kg IP) did not antagonize the protection afforded by atropine sulphate against metrazol. The results of the study suggest an involvement of central cholinergic mechanisms in metrazol-induced convulsions. | 117,491 | [
-0.04730877,
-0.1685594,
-0.0006541328,
-0.3321555,
-0.1027474,
-0.001476965,
-0.3072518,
-0.3657531,
-0.07105489,
-0.03367526,
0.06942239,
0.35223,
-0.07749768,
0.3554072,
-0.38499,
-0.1046658,
-0.5602548,
0.3663347,
0.0231237,
-0.002230105,
-0.2370368,
0.01227427,
0.131... |
[Experimental trachoma]. | During half a century, the agent of trachoma could be mainly demonstrated by inoculation to the conjunctiva of animals; by this mean the cycle of the agent could be revealed. There was a huge progress when T'ANG for these studies inoculated embryonated chicken eggs. However, experimentally infected animals are used at present time not only in trachome countries where do not exist laboratories: monkeys, guinea pigs, rabbits, rats and mice allow modern studies of chlamydial infection. Monkeys living in the countries where trachoma is endemic were selected because of their cheapness (orangoutan in Java, macaques in Northern Africa and in Taïwan, baboons in Africa). The monkeys selected by American workers are coming from South America. First pioneers (NICOLLE, CUENOD and NATAF, PAGES, JULIANELLE) have demonstrated the infectivity of animals and the place of the agent of trachoma on taxonomic point of view. As PAGES, we have demonstrated that infection could be regularly provoked when inoculating macaques; moreover a pannus could appear when adding hydrocortison drops or when infiltrating the cornea with tuberculin. Cultures of WEEKS bacilli were introduced in the eyes of trachomatous animals; we could observe an aggravation of the disease. If biology of trachoma is better known at present time, experimental trachoma is until now fundamentally important. It permits immunological studies especially for the purpose of vaccination; one can check terapeutical means for instance antibiotics; studies are performed to demonstrate cross immunizations or enhancement of defence (with levamisole). Experimental trachoma is hitherto and again for a long time commonly requested for the study of trachoma. | 117,538 | [
-0.1685403,
-0.3496607,
-0.2444398,
-0.4248499,
0.08700596,
-0.4180153,
-0.3172736,
0.05099743,
0.04052576,
0.09185051,
-0.0203251,
-0.01575956,
0.3514954,
-0.1460779,
-0.2573059,
-0.02919894,
-0.2585967,
0.1414933,
0.009147328,
0.539551,
0.2000351,
-0.002621005,
-0.03248... |
[Is the use of large amounts of glucose in parenteral feeding a problem?]. | Intravenous feeding with glucose as the sole carbohydrate was analyzed in a retrospective study of 30 patients. Very good assimilation of intravenous glucose without dangerous blood sugar variations could be demonstrated. As the administration of glucose was increased stepwise in the initial phase, the incidence of glucose intolerance was minimal. The good correlation of blood and urinary sugar shows that there is no problem in the control of carbohydrate metabolism during intravenous alimentation with glucose, and that urine samples provide adequate control after the initial phase. | 117,546 | [
-0.1482029,
-0.01256407,
-0.1781644,
-0.1554862,
0.2683601,
-0.386872,
-0.3560964,
0.05274125,
-0.2941614,
-0.02749467,
0.2864887,
-0.1794567,
-0.03463191,
-0.25444,
-0.1023027,
-0.2647627,
-0.5050027,
0.1440135,
0.02928928,
0.04915456,
0.2323977,
-0.004103626,
0.09005025... |
Highlights of physiological research at the University of Cape Town, 1912-1978. | During the 68 years since it was founded, 548 publications have appeared from the Department of Physiology of the University of Cape Town, which has at different times included subdepartments of pharmacology and of medical biochemistry. The main fields of physiological research have been electrophysiology of the heart and of nerves, calcium metabolism (especially of teeth), endocrinology (especially of sex hormones), phonocardiography, bile secretion, exercise physiology (especially tests of physical fitness), measurement of obesity, renal physiology (especially membrane transport of sodium) and neurophysiology. Work continues in some of these fields. | 117,559 | [
0.02381524,
-0.1072111,
-0.4353265,
0.1708976,
-0.1425541,
-0.6465269,
-0.4952263,
0.06135841,
0.02900464,
-0.1333272,
0.1211061,
-0.1055447,
0.1888025,
-0.1913757,
-0.485054,
-0.3338165,
-0.1056416,
-0.1045323,
-0.4344175,
0.2967201,
0.08024,
0.1186751,
-0.2726039,
0.1... |
[Use of the ECG in bovine cysticerosis]. | In two calves with massive cysticercosis and one healthy control calf the method of electrocardiographic examination was applied to a preciser determination of the clinical finding. The examination was performed on the 75th day after the experimental infection. Singly recorded electrocardiographic findings provided no characteristic symptoms indicating an injury of the conductive system of the heart. However, in both infected calves a lowering of the pulse frequency was found and in one of them also a flattening and a change of the polarity of the T wave. Autopsy and histological examination of the heart of the infected calves performed 111 days and 250 days after the infection revealed an attacking of the cardiac muscle by cysticerci and dispersed chronic myocarditis. This finding and the investigated dynamics of pathological changes in the course of cysticercosis in other experimental animals indicate that at the time of the electrocardiographic examination cysticercoid myocarditis at a stage of rest was recorded. | 117,596 | [
-0.1914505,
0.1718479,
0.002159645,
-0.1992144,
-0.07288192,
-0.4631206,
-0.2166609,
0.06164372,
-0.08103781,
-0.08185104,
0.06836776,
0.3540271,
-0.0292602,
0.1456306,
-0.3872226,
-0.1692839,
-0.6437171,
-0.1289673,
-0.01102815,
-0.01674528,
0.147499,
0.3205957,
-0.10282... |
[Ultrastructure of the double and multiple flagella of the sperm of the drake]. | The structure of double and multiple flagella of the spermatozoa of a drake was investigated. In spermatozoa with this defect one or two implantation pits were observed in the head of the spermatozoon with one or two proximal centrioles situated in the extended base of the nucleus. The structure of the proximal centrioles was frequently disturbed. Distal centrioles were placed separately without any marked figural or structural disturbance. The mitochondrial sheath was formed around both or more flagella, and between the flagella only one line of mitochondria was usually found. The ring showed defects in shape and changes in the deposition. An amorphous sheath was formed around each flagellum separately without any marked defects. In single cases insignificant changes were recorded in the arrangement of fibrils of the axial bundle. | 117,599 | [
0.07502299,
0.6451228,
-0.07736573,
0.1993895,
0.06497655,
-0.0858397,
-0.02566986,
0.1618125,
0.1023395,
0.460279,
0.1868129,
0.007037774,
-0.1023404,
0.3366308,
0.01789476,
-0.001609757,
-0.7241673,
-0.07252926,
-0.03033535,
-0.3374551,
0.5777541,
0.3001906,
0.09614196,... |
[Glycoproteins in the blood of dogs after administration of beta, beta'-dichlorodiethylsulphide]. | The protein spectrum of the dog blood serum was studied after the administration of beta,beta'-dichlorodiethylsulphide. Paper electrophoresis was used for this purpose. Fuchsin-positive substances were determined after previous oxidation with periodic acid, which made it possible to reveal that the glycoproteins detected in this way were represented in serum proteins mostly in the fraction of alpha 2 globulins. After beta, beta'-dichlorodiethylsulphide intoxication the values of these glycoproteins significantly increase. The rise is statistically significant 24 hours after administration (average rise by 141%) and within the time scope it reaches its maximum in the terminal stage (rise by 210%). | 117,600 | [
-0.3767717,
-0.1954683,
-0.04739406,
-0.1059338,
0.2753904,
-0.2974239,
-0.09544624,
-0.0239586,
-0.1314154,
0.1987139,
0.1034806,
0.2044257,
0.1380824,
-0.2503392,
-0.1005009,
-0.2078043,
-0.2726225,
0.162204,
0.3594703,
0.2842993,
0.04228269,
0.1239595,
-0.3235575,
-0... |
[Isotachophoretic isolation of IgD and IgE and production of monospecific antisera against them]. | The method of preparative isotachophoresis in acrylamide gel ensuring a high yield of IgD and IgE with insignificant admixtures of IgG, etc. was used for the isolation of IgD and IgE from the blood sera of myeloma patients. As a result of immunization with these antigens, monospecific IgD and IgE antisera were obtained. These antisera, alongside with specific antibodies, contained antibodies to admixtures; the latter were eliminated by the method of immune absorbtion carried out with the use of a sorbent based on Sepharose activated with bromo-cyanogen and conjugated with normal human blood serum. Ig D antisera were also shown to contain antibodies to idiotypical IgD determinants located in the Fab fragment of this immunoglobulin. | 117,652 | [
-0.1766426,
0.0961879,
0.0628689,
0.2064883,
0.2136576,
-0.2907495,
-0.35299,
0.2254843,
-0.02276234,
0.4860944,
0.3059731,
0.1841608,
0.1136015,
0.1506379,
-0.5467345,
-0.1367614,
-0.3617968,
-0.04667598,
0.01214143,
0.1662309,
0.4167578,
-0.1165275,
-0.1950347,
-0.042... |
[Immunostimulating activity of the lipopolysaccharides of blue-green algae]. | The whole cells of blue-gree algae and lipopolysaccharides isolated from these cells were shown to stimulate the production of macro-(mainly) and microglobulin antibodies in rabbits. The macro- and microphage indices in rabbits increased significantly after the injection of LPS isolated from blue-green algae 24--48 hours before infecting the animals with a virulent Y. pseudotuberculosis strain. Besides, the inhibiting action of this strain on the migration of phagocytes to the site of infection was abolished immediately after the injection. The use of the indirect hemagglutination test allowed to prove the absence of close antigenic interrelations between blue-green algae and the following organisms: Spirulina platensis, Microcystis aeruginosa, Phormidium africanum and P. uncinatum. | 117,655 | [
-0.1948418,
-0.3004839,
-0.116151,
0.269265,
-0.3324364,
-0.0424318,
-0.4121272,
0.2558729,
-0.1320754,
-0.1253122,
-0.0483739,
0.1522994,
-0.002525621,
-0.0004540326,
-0.5041628,
0.05937537,
-0.337617,
0.1231651,
0.1521332,
0.2217363,
0.6261872,
0.1369746,
0.1485891,
-... |
A TSH secreting pituitary tumour causing hyperthyroidism: presentation of a case and review of the literature. | A 45 year old male with a 12 year history of mild hyperthyroidism and a pituitary tumour is presented. He had both clinical and laboratory evidence of hyperthyroidism and his serum TSH was persistently and markedly elevated. A TRH test resulted in no further rise in serum TSH. No evidence of pituitary or peripheral endocrine deficiencies existed and prolactin levels were normal. Craniotomy was performed and a pituitary adenoma was removed. On light microscopy, it was mostly composed of chromophobes. However, occasional granulated cells were observed, and on electron microscopy, most of the cells contained fine granules, which suggested possible thyrotroph origin of the tumour. One week post-operatively the patient's serum TSH returned to normal. Again, TRH produced no response in TSH. The patient became hypothyroid by clinical and laboratory findings and is currently on thyroid replacement therapy. The previously reported TSH secreting tumours associated with hyperthyroidism are reviewed. | 117,663 | [
0.1447454,
-0.1459832,
-0.2043657,
-0.3273792,
-0.04401231,
-0.3007566,
-0.2127499,
-0.02553741,
0.1232073,
0.3031046,
0.3672894,
0.1168932,
-0.1591337,
0.0705191,
0.01136291,
-0.04755065,
-0.07350904,
-0.08396757,
0.245694,
-0.1093517,
-0.1577073,
0.216963,
-0.03426189,
... |
Pseudothrombocytopenia due to agglutinins. | Pseudothrombocytopenia may have any of a number of causes, one of which is agglutination in vitro. This phenomenon was found in samples of blood from six patients. A serum factor responsible for the agglutination was demonstrated. The factor was dependent upon the presence of EDTA and was more active at room temperature than at 37 C. It could be identified as an IgM immunoglobulin in four cases. In the other two cases definite characterization was not possible, but there was some evidence in favor of an IgM factor. All six patients had elevated serum IgM levels, but they had different and unrelated clinical disorders. | 117,695 | [
-0.1312285,
-0.1344387,
-0.1393324,
-0.1436534,
0.1606663,
-0.1502944,
0.02751745,
0.1741298,
-0.2365092,
0.2399588,
0.057773,
0.4866464,
0.2587463,
-0.2492066,
0.05746818,
-0.2415285,
-0.1550074,
-0.08263128,
-0.1497635,
0.4415705,
0.1882617,
0.07412818,
-0.02044317,
0... |
Dyggve-Melchior-Clausen syndrome: genetic studies and report of affected sibs. | We report a brother and sister with Dyggye-Melchior-Clausen dysplasia with mental retardation (MR) but as yet without spinal cord injury due to cervical spine abnormality. Mucopolysaccharide metabolism was studied in several ways and was found to be normal. Segregation analysis and study of consanguinity data confirm that both forms of the syndrome--that with MR, and that without MR (Smith-McCort dysplasia) are rare autosomal recessives. Spinal cord injury and early death is a danger in both. | 117,710 | [
0.001574609,
-0.1751606,
0.1698587,
-0.326839,
0.03838469,
-0.568046,
-0.3979079,
-0.1935526,
-0.0850004,
0.1731658,
0.01820481,
0.5703995,
-0.09955779,
-0.02760489,
-0.2185478,
-0.04367561,
-0.3314264,
-0.03133012,
0.1202579,
-0.1733836,
0.7060938,
0.1535618,
-0.229014,
... |
Cricopharyngeus myotomy as the only treatment for Zenker diverticulum. | Cricopharyngeal dysfunction, one of the most common causes of pharyngeal dysphagia, exhibits a variety of manifestations, one of which is Zenker diverticulum. This paper examines the physiology of swallowing, pathophysiology of its aberrations, and various methods of treating Zenker diverticulum. It is our purpose to emphasize cricopharyngeus (CP) myotomy as the only needed treatment for this diverticulum. Even in its advanced stages, excision of the diverticulum is a needless surgical exercise. Seven cases of Zenker diverticulum are reported in elderly patients; one of them had an excision of the diverticulum prior to presentation. Some were either completely obstructed or aspirating on esophagram. Cricopharyngeus myotomy, the only treatment provided, proved to be safe and effective without morbidity or fatalities. Patients' ability to eat orally was restored on the night of or the morning after surgery. No Levin tube is necessary and there is no risk of suture line leakage after the conventional diverticulectomy and CP myotomy. Hospital stay is greatly reduced and there is no risk of structure formation. In contrast to endoscopic division of CP muscle, there is no risk of mediastinitis because there is no break through the mucosa. | 117,733 | [
-0.2695157,
-0.02854785,
-0.2043645,
-0.2020219,
-0.1723929,
-0.1087328,
-0.410886,
-0.1205427,
0.3374849,
-0.01481155,
0.1907743,
-0.1448161,
-0.09147803,
-0.4249161,
-0.08618457,
0.2129566,
-0.1605935,
0.07965551,
-0.179148,
-0.01916446,
0.3221445,
-0.1257706,
0.0073006... |
Chemical characterization of a new Japanese variant of carbonic anhydrase I, CA INagasaki 1 (76 arg leads to gln). | A new inherited variant of carbonic anhydrase I (CA I), designated CA INagasaki 1 (CA INGS 1), was discovered during a survey of hemolysates from 5852 individuals from the cities of Hiroshima and Nagasaki in Japan. Analysis of the amino acid composition of a tryptic peptide from the CA INGS 1 variant indicated that a glutaminyl residue was substituted for an arginyl residue at position 76. Heat degradation studies showed that the CA INGS 1 variant was less stable than normal CA I. The CO2 hydrase and esterase activities of the normal and variant carbonic anhydrases I, as well as the relative amounts of the two enzymes in heterozygotes, were similar. | 117,793 | [
-0.1489382,
0.1223716,
-0.2120857,
0.07864742,
-0.3974129,
0.09952249,
0.0811087,
0.001008913,
0.3243305,
0.2682705,
0.28608,
0.3077078,
-0.2739833,
-0.3986366,
-0.08390202,
-0.1343233,
-0.6007631,
0.28516,
0.312754,
0.1622932,
0.07142256,
0.5577103,
-0.1390016,
0.16147... |
Purification and enzymatic properties of lysyl hydroxylase from fetal porcine skin. | Lysyl hydroxylase from fetal porcine skin is shown to bind in a highly specific manner to aminoethyl-Sepharose 4B. When coupled to ammonium sulfate fractionation and DEAE-cellulose chromatography, chromatography of lysyl hydroxylase preparations on aminoethyl-Sepharose 4B has yielded a highly purified (greater than 95%) preparation of lysyl hydroxylase. The enzyme consists of two subunits with molecular weights of 70 000 and 115 000. The overall recovery of activity was 2.5%, yielding approximately to 3.5 mg of purified enzyme from 900 g of fetal porcine skin. The enzyme is more active at 30 degrees C than at 37 degrees C and has a pH optimum near 8.0. Both catalase and bovine serum albumin are required by the enzyme for maximum activity. The sulfhydryl reagents p-(chloromercuri)-benzoate, N-ethylmaleimide, and iodoacetamide are potent inhibitors of the enzyme, whereas dithiothreitol appears to be an activator. | 117,833 | [
-0.1416307,
-0.1544737,
0.04592676,
0.1309161,
0.07346705,
0.06031598,
-0.3126084,
-0.0777754,
0.2391908,
0.08720911,
0.04917988,
0.05316776,
0.1926571,
0.1585132,
-0.1925223,
0.2207954,
-0.62123,
0.2335881,
0.02520122,
0.4376563,
0.1241494,
0.4812089,
-0.3413711,
0.048... |
Evidence for the resistance of thyrotropin-releasing hormone (TRH) and pseudo-hormone, pyroglutamyl histidyl-amphetamine, to degradation by enzymes of the digestive tract in vitro. | TRH and pseudo-hormone (pyro Glu-His-amphetamine) were submitted to the digestion of chymotrypsin and prolidase and independently to the digestion of enzymes of the digestive track: pepsin (stomach), pancreatins (pancreas) and enzymes extracted from the intestinal mucosa (small intestine). Using thin layer chromatography and high voltage electrophoresis techniques to detect enzymic digestion products, only intact TRH and pseudo-hormone were found, indicating that both entities were, under the conditions used, resistant to in vitro digestion by enzymes of the digestive tract. | 117,843 | [
0.03044832,
-0.5778918,
-0.2972341,
0.1492359,
-0.1125926,
-0.1498787,
0.06549943,
-0.06219821,
-0.1261034,
0.2316163,
0.3313124,
-0.2853186,
0.03153301,
0.3041103,
-0.5395547,
0.2743788,
-0.2937964,
-0.02077392,
-0.02428382,
0.1635886,
0.02568196,
-0.2395978,
0.07150245,... |
Immunohistochemical localization of thyrotropin-releasing hormone (TRH) in skin of Rana pipiens. | Using immunofluorescent techniques thyrotropin releasing hormone (TRH) is demonstrated in skin of Rana pipiens and R. catesbeiana. The immunofluorescent-TRH is localized in all cell layers of the epidermis and in the epithelium lining the various cutaneous glands, but not in the dermal layer. | 117,895 | [
0.4415928,
-0.5113882,
-0.2025801,
-0.01250486,
-0.174231,
-0.1033846,
-0.1862404,
-0.1675623,
-0.05938502,
0.2517084,
0.1677601,
-0.03156617,
0.1108471,
-0.0101854,
-0.2800749,
-0.2746709,
-0.02790249,
0.2558239,
0.3327584,
-0.1862791,
0.2378689,
0.1953731,
-0.1623958,
... |
In vitro induced pinocytotic activity by a juvenile hormone analogue in oocytes of Drosophila melanogaster. | Pinocytotic activity has been analyzed in Drosophila oocytes following either in vivo or in vitro exposure to horseradish peroxidase. The enzyme tracer gains access to the yolk spheres only when supplied to the oocyte in vivo. In oocytes cultured in vitro, peroxidase remains restricted to the residual coated vesicles and to the tubular profiles formed in excess in the cortical ooplasm. In an attempt to induce peroxidase uptake by oocytes cultured in vitro, various incubations were tested. Among these, hemolymph from both sexes is capable of promoting peroxidase uptake up to a level comparable to that detectable in vivo. On the other hand, fat body extracts fail to promote such cellular activity. Finally, the juvenile hormone analogue ZR-515 is shown to be the only factor required to promote pinocytotic activity under the experimental conditions tested. The observations are interpreted to indicate that vitellogenin has no inductive role on pinocytosis but simply acts by adhering to the forming coated vesicles which in turn are produced by the oolemma in response to the action of juvenile hormone. | 117,896 | [
0.1674004,
-0.3417996,
-0.1551019,
-0.04754993,
0.001826171,
-0.04578439,
0.1061133,
0.1795242,
-0.01376612,
0.2408361,
0.06275559,
0.1054884,
0.02495734,
-0.4221979,
-0.3489523,
0.1384385,
-0.4882805,
0.09861401,
0.3175601,
-0.09206541,
0.4302427,
0.2378968,
-0.06160142,... |
[Unmasking of the fast sodium channel in less than 4 day old embryonic chicken heart by inhibitors of sodium inactivation]. | Embryonic Chick hearts aged less than 4 days are not always sensitive to tetrodotoxin, an inhibitor of fast sodium channel. It is shown that in the most frequent cases, in which tetrodotoxin sensitivity is apparently absent, this sensitivity can be demonstrated after previous treatment by veratridine or by toxin II of androctonus australis Hector Scorpion venom. It is concluded that the fast tetrodotoxin-sensitive sodium channel is regularly present in the heart of Chick embryos aged 2 and 3 days, but most often in a permanently inactivated state. | 117,932 | [
-0.02127392,
0.16182,
-0.5500723,
-0.258563,
0.2786349,
-0.1990137,
0.02775984,
-0.03524148,
0.02292246,
-0.07071355,
0.2692563,
0.5439515,
-0.009774613,
-0.4055371,
-0.2097027,
-0.5673734,
-0.8320038,
0.0964324,
-0.0251711,
-0.1393787,
0.4935131,
0.266518,
-0.2979138,
... |
[Effect of cell proliferation on the condensation of the X chromosome in culture medium of the individual karyotype 49 XXXXX]. | The influence of cell proliferation on the condensation of the X chromosome was observed in vitro in human fibroblasts with 49 XXXXX karyotype. The frequency of cells with four Barr-bodies is low during the logarithmic growth phase and increases to 80% when the cells are becoming confluent or, independently of cell contact, when cell growth is arrested in a medium with low serum content. The condensation of th X chromosomes is reversible when the cells start growing again in medium with a higher serum content. | 117,944 | [
-0.04415416,
-0.1350348,
0.1723368,
-0.1227135,
0.1827548,
-0.3026958,
-0.3997622,
0.1726362,
0.03319112,
0.2516432,
-0.03878704,
0.1749487,
-0.1933988,
0.1163974,
-0.9293982,
-0.4446954,
-0.3273356,
-0.205788,
-0.1424512,
0.3095956,
0.6773694,
0.1279228,
-0.1747761,
-0... |
[Isolation of the yellow fever virus from an egg-cluster and the larvae of the tick Amblyomma variegatum]. | The yellow fever virus is isolated in natura from eggs of a Tick Amblyomma variegatum. It is then isolated from larvae issued from the same egg-cluster and also from blood of a monkey bitten by larvae of the same origin. It is reported that the same virus has been previously obtained from adults of the same species of Tick. An acarine appears for the first time as a sylvatic vector and reservoir (at least temporary) of yellow fever. | 117,946 | [
-0.1270186,
0.1407597,
0.05051945,
-0.07403401,
-0.02584196,
-0.266318,
-0.3157911,
-0.1143294,
-0.1991754,
-0.2059817,
0.2433958,
0.07829113,
-0.04886499,
-0.4050318,
-0.3904847,
-0.1889181,
-0.2184908,
0.4403756,
0.2324734,
0.0880265,
0.1159265,
-0.0395847,
-0.2009009,
... |
The effect of lisuride hydrogen maleate, an ergot derivative on anterior pituitary hormone secretion in man. | The effects of single oral doses of 0.2 mg of lisuride hydrogen maleate, a semisynthetic ergot derivative, on serum levels of prolactin (PRL), growth hormone (GH), thyroid stimulating hormone (TSH), luteinizing hormone (LH), follicle stimulating hormone (FSH), cortisol and blood glucose were studied in six normal males. Lisuride effectively inhibited basal PRL secretion as well as the PRL response to TRH given 3 h later. In addition, the drug raised basal GH levels and decreased basal and TRH stimulated TSH secretion. No significant differences between lisuride and control were observed in basal LH and FSH, LHRH stimulated gonadotrophins or in cortisol. Drowsiness was noted by all subjects, one became nauseated and another vomited, 60 and 90 min respectively after administration of lisuride. No changes were seen in pulse rate and blood pressure. The endocrine effects of lisuride were attenuated by the prior administration of the dopamine antagonist metoclopramide. These results suggest that lisuride acts as a long-acting dopamine agonist and that therefore this drug could be of therapeutic use in hyperprolactinaemic states and acromegaly. | 117,952 | [
-0.254197,
-0.01038103,
-0.1714048,
-0.1501558,
0.293042,
-0.2137978,
-0.01508582,
-0.3556351,
-0.1872514,
-0.172835,
0.1590512,
0.1182329,
-0.03265791,
0.1617292,
-0.2057907,
0.01269806,
-0.4814771,
0.1908102,
-0.151899,
0.125205,
-0.1470358,
0.03158592,
-0.05830809,
-... |
A simplified technique to prepare fungal specimens for scanning electron microscopy. | A simplified technique to prepare fungal specimens for scanning electron microscopy is described and discussed. Fixation in either 6% glutaraldehyde or 2% Os04 (both unbuffered aqueous solutions), yielded good results. The minimum fixation time in OSO4 was 2 h, in glutaraldehyde 4 to 5 h. Chemical dehydration with 2-methoxyethanol or 2,2-dimethoxypropane proved to be useful since it considerably reduced the preparation time. Because of the relatively few changes of reagent solutions during the whole process, the fungal specimens were less disturbed without much loss of material. The preparation technique described has been applied to specimens of various fungal groups with good results. | 117,981 | [
-0.3378152,
0.05042586,
-0.0887217,
-0.1460676,
0.07038398,
-0.2321094,
0.06217026,
0.01914697,
0.1894467,
-0.02604957,
0.1491407,
0.1087134,
0.04122228,
-0.02412554,
-0.4518829,
0.02104166,
-0.2748395,
0.1697213,
-0.119146,
0.2140215,
0.192762,
0.186105,
-0.1294481,
-0... |
Episodic and TRH induced growth hormone release in primary hypothyroidism of man and rat. | In 27 hypothyroid subjects studied over 20 to 120 minutes, the concentration of serum growth hormone (GH) was variable with the amplitude and frequency of the secretory patterns similar to those reported by others for normal individuals. Serum GH, after the administration of thyrotropin releasing hormone (TRH) did not differ from values observed as spontaneous surges, in contrast to a consistent increase in thyrotropin and prolactin. Episodic secretion of GH persisted in thyroidectomized rats and did not differ significantly from that present in intact controls. It is concluded that episodic GH secretion is not abolished in primary hypothyroidism and that TRH is not a constant GH secretagogue in human subjects with hypothyroidism. | 118,000 | [
-0.02432174,
-0.2682928,
-0.4345639,
-0.3331958,
0.09583569,
-0.5497597,
-0.1093896,
-0.3274754,
-0.1467707,
0.1230824,
0.1884912,
0.4484197,
-0.1628361,
-0.2448749,
0.03851799,
-0.2182432,
-0.1997683,
-0.1262808,
0.04638268,
-0.0769807,
0.07562265,
0.2195001,
-0.00504335... |
Vestibular nuclei activity in the alert monkey during suppression of vestibular and optokinetic nystagmus. | Single neurons were recorded in the vestibular nuclei of monkeys trained to suppress nystagmus by visual fixation during vestibular or optokinetic stimulation. During optokinetic nystagmus vestibular nuclei neurons exhibit frequency changes. With the suppression of optokinetic nystagmus this neuronal activity on average is attenuated by 40% at stimulus velocities of 40 degrees/s. At a stimulus velocity of 5 degrees/s responses are, under both conditions, close to threshold. For steps in velocity, suppression of vestibular nystagmus shortens the time constants of the decay of neuronal activity from 15--35 s to 5--9 s, while the amplitude of the response remains unchanged. The results are discussed in relation to current models of visual-vestibular interaction. These models use a feedback mechanism which normally operates during vestibular and optokinetic nystagmus. Nystagmus suppression interrupts this feedback loop. | 118,047 | [
-0.2456409,
-0.1693892,
-0.3892498,
-0.2925677,
0.2675122,
-0.3834348,
-0.2185149,
-0.2870822,
0.1974847,
-0.01845661,
-0.09596693,
0.06990234,
-0.1176366,
-0.1704923,
-0.2625673,
-0.1935928,
-0.6610785,
0.08114064,
-0.1854163,
0.1009249,
0.146153,
0.03584715,
0.01063548,... |
Phenytoin serum levels in children with epilepsy: a micro immuno-assay technique. | The enzyme multiple immuno-assay technique (EMIT) was used to study phenytoin serum levels in 50 children with seizures. It was found that: (1) a single dose of phenytoin suspension or capsules (5mg/kg/day) produced inadequate serum levels 16 and 24 hours after ingestion, and for this reason single dosage is not recommended; (2) twice-daily dosage of phenytoin suspension or capsules (5mg/kg/day) produced adequate serum levels in most children throughout the 24 hours, and this dosage is recommended; (3) 12 children continued to have seizures but when the dose was increased to 10mg/kg/day six of the 12 obtained control of seizures; (4) phenytoin reached equilibrium in the serum in five days provided the child had not previously been taking phenobarbitone; (5) of 13 children who had been taking phenobarbitone, 10 did not achieve equilibrium of phenytoin in serum for one to four weeks; (6) phenytoin suspension given twice-daily produced satisfactory serum levels provided the bottle was shaken well before dispensing; (7) apart from minor variations, phenytoin maintained its level in serum during the 14 to 30 months follow-up period, whether 5mg or 10mg/kg/day of phenytoin was given. | 118,073 | [
-0.2429734,
0.3757769,
-0.2067105,
-0.207698,
-0.3088347,
-0.2292978,
-0.6314507,
0.1824722,
-0.04300755,
-0.2020806,
0.1570365,
0.4390893,
-0.02548558,
0.3524576,
-0.1843568,
0.1816389,
-0.2540273,
0.3899075,
0.03653932,
0.3599036,
0.2793792,
0.1943265,
0.0003639957,
-... |
Evaluation of the teratogenicity of anticonvulsants. | Evaluation and comparison of the reports on teratogenicity of drugs are very difficult because many fundamental, clinical and social problems exist in the research of congenital anomalies and detection of human teratogens. Since the etiological significance of anti-convulsants has not been established in humans, the following efforts should be made extensively: 1) systematic collection and detailed analysis of more human cases, 2) study on the role of epilepsy and treatment with multiple drugs, 3) investigation on the mechanism of teratogenesis including comparative pharmacokinetic studies in animals and humans. | 118,096 | [
-0.02587308,
0.01073276,
-0.07787619,
-0.2284847,
0.1713048,
-0.2702696,
-0.3754536,
-0.2335024,
0.04417159,
0.03836646,
0.1625056,
0.07802153,
0.08591591,
0.3474877,
-0.1902793,
-0.01207088,
-0.3592709,
0.16813,
-0.01682136,
0.3176398,
0.0627519,
0.2634347,
-0.06910706,
... |
Immune mechanisms against canine distemper. II. Role of antibody in antigen modulation and prevention of intercellular and extracellular spread of canine distemper virus. | Specific antibody was shown to be highly effective in neutralizing extracellular canine distemper virus (CDV) as well as preventing the intercellular spread of this virus. Thus, relatively low levels of antibody neutralized 1 x 10(5) TCID50 of extracellular CDV and the development of plaques or CPE in Hep-2 and Vero cells respectively could be prevented even when up to 5% of the cells were infected. This inhibition of CPE and virus spread was most pronounced when antibody was added early but could still limit the degree of CPE if added as late as 48 h post-infection. This anti-viral activity was observed in different cell types including canine macrophages, cells normally infected with CDV in vivo. Prolonged exposure of infected target cells to high concentrations of antibody led to redistribution of surface viral antigens and their subsequent disappearance. The possible role of antibody in the defence against, and/or recovery from CDV and the mechanism(s) by which antibody may aid in recovery are discussed. | 118,114 | [
-0.1315959,
-0.003005612,
0.07310182,
-0.2297067,
0.1804106,
-0.188554,
0.2036521,
0.2421937,
-0.088756,
0.3304366,
0.1432828,
0.231052,
0.1146039,
0.005417749,
-0.4381481,
-0.2374166,
-0.4109076,
-0.05390792,
-0.05280872,
0.2071574,
-0.3575664,
0.2003682,
-0.2247475,
-... |
[Balanced parenteral and oral feeding of underweight newborn infants]. | The oral feeding of 50 newborns with low birth weight was combined with infusions of two different amino acid solutions (mother milk-adapted and requirement-adapted), parenteral lipids and glucose. All infants showed a rapid weight gain, the N-balance became positive, hypoglycemic or hyperosmotic reactions were missing. Amino acid imbalances could not be seen, values of glutamic acid, aspartic acid and asparagine differed in both groups just as proline. In management of infants with low birth weight the supplemental application of these amino acid solutions, lipids and glucose has advantages. | 118,117 | [
0.1534222,
-0.264069,
-0.1678168,
-0.111683,
0.2789817,
-0.08048278,
-0.05204467,
-0.2390351,
-0.03304914,
0.08975098,
-0.0248558,
0.2268528,
-0.009922672,
-0.140587,
-0.4321196,
-0.5227093,
-0.5550236,
0.2112195,
-0.1810123,
0.0780064,
0.2862481,
0.008612772,
0.1045284,
... |
Deoxyribonucleic acid repair capacities of Neisseria gonorrhoeae: absence of photoreactivation. | No difference in survival was observed when ultraviolet-irradiated gonococcal cells were subsequently incubated in the dark or exposed to photoreactivating light. This observation indicates that photoreactivation is absent in Neisseria gonorrhoeae. | 118,154 | [
-0.07226156,
-0.1386744,
-0.3603476,
-0.2267769,
0.01417551,
-0.06833431,
-0.1872975,
-0.1617043,
0.2855873,
-0.1390983,
0.151362,
0.1903859,
-0.06862754,
0.104995,
-0.3229793,
-0.07981261,
-0.4225802,
0.1456919,
-0.1908544,
0.2283214,
0.4665323,
0.07793587,
0.01971858,
... |
Synthesis of nitrogenase and heterocysts by Anabaena sp. CA in the presence of high levels of ammonia. | Anabaena sp. CA fails to synthesize heterocysts and nitrogenase when grown with KNO3 as the nitrogen source. By contrast, both heterocysts and proheterocysts are synthesized in NH4Cl-containing media to a level nearly commensurate with cells grown in the absence of combined nitrogen. The growth rate of the organism in NH4Cl-containing media was similar to that obtained with KNO3 as the nitrogen source and was independent of the presence of N2 in the atmosphere. Thus, our results indicate that the organism assimilated nitrate and ammonium nitrogen equally well to meet the nitrogen requirements for growth. Moreover, in contrast to previous studies with other cyanobacteria, the repressor singal for heterocyst differentiation in Anabaena sp. CA is not derived from the metabolism of ammonia but appears to be involved with nitrate metabolism. Nitrogenase activity was partially expressed in NH4Cl-grown cultures. Increasing the level of nitrogenase activity to a value representative of a N2-grown culture required both the inhibition of ammonia assimilation and de novo protein synthesis. An increase in the number of mature heterocysts was not required. The fact that high levels of exogenous ammonia only partially repress the synthesis of proteins required for the maximum expression of nitrogenase activity in Anabaena sp. CA has important implications. | 118,162 | [
-0.348495,
-0.1362241,
-0.2907759,
-0.1447407,
-0.4885874,
-0.1980411,
-0.1318565,
0.008306643,
0.2774481,
-0.2923842,
-0.2055656,
0.09752665,
0.1177154,
0.01500393,
-0.4589987,
0.1634361,
-0.3701542,
-0.1765153,
0.08568218,
-0.1931104,
0.5172308,
0.2092664,
-0.100179,
... |
Characterization of polyriboadenylate polymerase from Tetrahymena pyriformis. | Poly(A) polymerase [polyadenylate nucleotidyltransferase, EC 2.7.7.19] was extracted from Tetrahymena pyriformis. The enzyme was demonstrated to be present in three forms by column chromatography on DEAE-cellulose, and they were termed poly(A) polymerase Ia, Ib, and II in order of increasing affinity to the column. The properties of enzymes Ia and Ib were similar except that Ia utilizes poly(A) as a primer rather efficiently. Enzyme II differed from enzymes Ia and Ib not only in elution profile on DEAE-cellulose column chromatography but also in pH and temperature preferences, molecular weight, requirement for divalent cations, sensitivity to salts at high ionic strength, optimal primer concentration, and subcellular localization. The molecular weights of enzymes Ia and Ib measured by gel filtration were both 43,000, and that of enzyme II was 95,000. All three enzymes required Mn2+ for maximal activity; Mg2+ could replace Mn2+ in the reaction of enzyme II, but only partially. In the presence of 0.1 M ammonium sulfate the activities of enzymes Ia and Ib were both completely inhibited, whereas enzyme II still showed 42% of its original activity. These findings suggest that there are two distinct types of poly(A) polymerase in Tetrahymena pyriformis. | 118,167 | [
-0.1466632,
-0.1221618,
-0.1097964,
0.06171105,
-0.04210884,
-0.003759679,
-0.2637557,
0.217059,
0.05674177,
-0.1652742,
0.2026953,
-0.312708,
0.1428739,
0.01088778,
0.09893429,
0.1340961,
-0.1780663,
0.02354524,
0.2893024,
0.2852431,
0.4815081,
0.2489759,
-0.1408018,
0... |
Microbiology subsystem of a total, dedicated laboratory computer system. | The computer system used by the Microbiology Service of the Clinical Pathology Department, Clinical Center, National Institutes of Health is discussed. This microbiology subsystem is a part of a dedicated on-line laboratory computer system used by the entire department. The laboratory computer is connected on-line to a hospital computer which provides patient admission, transfer, and discharge data. Mark sense worksheets and cathode ray tube terminals are used for result entry and correction. Cumulative patient reports are printed. Results for both active and completed accessions can be easily retrieved on cathode ray terminals in the laboratory. All laboratory data are archived on magnetic tape from which a research data base and microfiched laboratory records are generated. The manner in which the system is integrated in the routine operation of the microbiology laboratory is emphasized. In addition, some of the costs, benefits, liabilities, and pitfalls associated with the introduction of the computer in the laboratory are reviewed. Finally, we have presented our concept of some of the future enhancements to our present system and some of the directions in which any future microbiology system might develop. | 118,177 | [
-0.08823615,
0.08726066,
0.1747154,
0.08777085,
-0.2173295,
-0.2468319,
-0.1302189,
0.2416933,
0.07804579,
0.01872875,
-0.09804321,
0.04185533,
0.1077855,
0.401246,
-0.5936416,
0.1993002,
0.02987671,
-0.09706369,
0.1811349,
-0.2440785,
0.2944245,
0.1994198,
-0.2854749,
... |
Antithrombin activities in childhood malnutrition. | Antithrombin activities in 30 severely malnourished children and 40 normal children were estimated in clotting tests by thrombin neutralisation as anti-Xa and by a heparin antithrombin assay; and by immunodiffusion as alpha 2-globulin and alpha 1-antitrypsin. The patients' mean alpha 2-globulin was severely depressed, and there were less marked depletions in mean values for thrombin neutralisation, anti-Xa, and in the heparin antithrombin assay (which showed the flat curve thought to reflect a thrombotic tendency). The alpha 1-antitrypsin values were normal. The findings support the concept of antithrombin as the summation of alpha 2-globulin and alpha 1-antitrypsin (with alpha 2-macroglobulin); and the low values may be related to the high incidence of thrombosis reported in childhood malnutrition, although it was not seen in these patients. | 118,190 | [
0.03479398,
0.1746362,
-0.3573835,
-0.01638896,
0.1686467,
-0.1325931,
-0.2719606,
0.1409208,
-0.2037057,
0.06659134,
0.1406431,
0.4542486,
-0.1072431,
0.05294119,
-0.1551083,
-0.0548959,
0.1172574,
0.2257942,
0.0929713,
0.1501326,
0.3793081,
-0.007110583,
-0.001106959,
... |
Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers. | Sequential treatment of rat cerebellar slices with two antisera, each specific for a different glial cell marker, revealed, by indirect immunofluroescence combined with the immunoperoxidase method on the same tissue section, that carbonic anhydrase isoenzyme II (CA II) is exclusively localized in oligodendrocytes while the glial fibrillary acidic protein (GFA) is, as well established, a specific marker for astrocytes. | 118,210 | [
0.05667092,
-0.2333577,
0.09799296,
-0.1281578,
0.06575353,
-0.2421629,
-0.173618,
-0.08719738,
0.07846565,
0.09575849,
-0.1627624,
0.3035952,
0.2127405,
-0.02514894,
-0.1515066,
-0.1247066,
-0.3214167,
0.07914098,
-0.3778188,
0.2348142,
-0.07589447,
0.1018143,
-0.1679651... |
Characterization of infection and replication of Mason-Pfizer monkey virus in human cell cultures. | Human cells derived from both normal and neoplastic tissues can be infected by Mason-Pfizer monkey virus (MPMV) without accompanying cytopathology. Infection of cell cultures such as human rhabdomyosarcoma (A204) results in a persistenly infected cell line which can be subcultured over 30 sequential culture passages without significant change in phenotype properties according to reverse, transcriptase (RT), MPMV p27 antigen content, virus particle count and infectivity titre. Productive virus infections were established at relatively low virus particle (VP) input multiplicities (p.i.m.; about 0.06 VP/cell) In A204 cell cultures. At higher p.i.m. (about 600 to 6000 VP/cell) newly synthesized virus was detected within 4 days post infection. Although virus production was cumulative following primary infection, after subculture of infected cultures MPVM production was greater during active cell division. Using synchronization techniques, MPMV replication in persistently infected cultures was found to be cell cycle-dependent. The major internal antigen, p27, was synthesized in G2 and newly synthesized virus particles were released predominantly during mitosis and early G1. Colcemid arrest of cells during mitosis inhibited subsequent MPMV release. Consequently, production of extracellular virus depends upon the progression of cells through the mitotic stage. These data, which provided a basic understanding of the virus-host relationship that occurs in primate cells productively infected with MPMV, were used as a guideline for isolating MPMV-like viruses from experimentally and naturally infected Rhesus monkey. | 118,235 | [
-0.4904629,
-0.05256896,
-0.05912114,
-0.3226991,
0.1783319,
-0.03331636,
-0.193891,
0.2671983,
0.09852803,
0.04774637,
-0.0418075,
0.1117848,
0.00001101506,
0.1619464,
-0.49548,
-0.03549345,
-0.1903906,
-0.2247234,
-0.05342225,
0.1772916,
0.4329563,
-0.09790568,
-0.17340... |
Changes in the virus-host cell relationship in a stable non-virogenic cell line persistently infected with measles virus (BGM/MV). | A non-virogenic African green monkey kidney cell line BGM/MV persistently infected with a neurotropic mouse brain-adapted strain of measles virus, was found to have undergone significant changes in the virus-host cell relationship between passages 35 and 119. Rather than the stable non-cytopathic relationship previously reported in which approximately 100% of the cells contained measles antigens and less than 1% of the cells expressed cell surface measles antigen, we observed cyclic manifestations of c.p.e. together with changes in the percentage of cells expressing intracellular and cell surface measles antigens. Treatment of BGM/MV cells with actinomycin D effected an increase in the percentage of cells expressing cell surface virus haemagglutinin (HA) at times when the percentage of cells with surface HA was less than the percentage of cells with intracellular measles antigens. Superinfection studies employing measles virus and vesicular stomatitis virus revealed a consonant cyclic refractivity and essentially no refractivity, respectively. Endogenous, infectious measles virus was not detected nor was interferon. It was concluded that a host cell factor other than interferon was modulating the cyclic expression of the measles virus infection. | 118,238 | [
-0.5226394,
-0.2723854,
-0.1244733,
0.2408003,
0.306067,
-0.127875,
-0.2217695,
0.2746099,
-0.02976423,
-0.2424593,
-0.2093152,
0.5563492,
0.08677739,
-0.2364447,
-0.2176557,
-0.2233019,
-0.1988199,
-0.1068488,
-0.1114738,
0.117273,
0.3537795,
-0.2151534,
-0.1201204,
-0... |
Co-translational modification of nascent immunoglobulin heavy and light chains. | We have investigated the in vivo co-translational covalent modification of nascent immunoglobulin heavy and light chains. Nascent polypeptides were separated from completed polypeptides by ion-exchange chromatography of solubilized ribosomes on QAE-Sephadex. First, we have demonstrated that MPC 11 nascent heavy chains are quantitatively glycosylated very soon after the asparaginyl acceptor site passes through the membrane into the cisterna of the rough endoplasmic reticulum. Nonglycosylated completed heavy chains of various classes cannot be glycosylated after release from the ribosome, due either to rapid intramolecular folding and/or intermolecular assembly, which cause the acceptor site to become unavailable for the glycosylation enzyme. Second, we have shown that the formation of the correct intrachain disulfide loop within the first light chain domain occurs rapidly and quantitatively as soon as the appropriate cysteine residues of the nascent light chain pass through the membrane into the cisterna of the endoplasmic reticulum. The intrachain disulfide loop in the second or constant region domain of the light chain is not formed on nascent chains, because one of the cysteine residues involved in this disulfide bond does not pass through the endoplasmic reticulum membrane prior to chain completion and release from the ribosome. Third, we have demonstrated that some of the initial covalent assembly (formation of interchain disulfide bonds) occurs on nascent heavy chains prior to their release from the ribosome. The results are consistent with the pathway of covalent assembly of the cell line, in that completed light chains are assembled onto nascent heavy chains in MPC 11 cells (IgG2b), where a heavy-light half molecule is the major initial covalent intermediate; and completed heavy chains are assembled onto nascent heavy chains in MOPC 21 cells (IgG1), where a heavy chain dimer is the major initial disulfide linked intermediate. | 118,307 | [
-0.1002031,
0.1421278,
-0.1861624,
-0.03671011,
0.02107825,
-0.1239706,
-0.03675738,
0.1641836,
0.06434625,
0.4919754,
-0.001682262,
-0.0327888,
0.18725,
0.05998625,
-0.1550773,
-0.05946387,
-0.4662553,
-0.281868,
-0.05007489,
-0.03752735,
0.7006777,
-0.003980125,
0.07914... |
[A controlled study on the significance of occult blood test in the stool (author's transl)]. | 1000 patients of a proctological practice were all investigated by coloscopy and parallel to this by comparison of the two tests for occult blood in the stool (Haemoccult and hemo FEC). 5,6% were positive by the Haemoccult test, 5,2% to hemo FEC. There was no significant difference between the two systems. In the entire study 13 carcinomata and 39 benign polyps of 5 mm diameter and more were found, 8 patients with carcinoma had a positive hemo FEC test, 7 had a positive Haemoccult test, i.e. 38% of carcinoma patients were not detected by the hemo FEC test and 46% in the Haemoccult test. Even in patients with somewhat larger polyps, the test for occult blood in the stool was negative in 56%. It is pointed out with reference to two separate patients that in individual cases of even advanced carcinoma of the large intestine no blood can be detected in the stool. | 118,339 | [
0.07472249,
-0.1176083,
-0.104897,
0.00430799,
-0.210622,
-0.5981812,
-0.01753485,
0.1249963,
0.003299871,
-0.1960808,
0.1816106,
0.4591615,
0.1002344,
-0.3150477,
-0.5022036,
-0.2434501,
-0.374933,
0.1457508,
0.1507765,
0.2088529,
0.369767,
0.06020392,
-0.164495,
0.363... |
Mutagen-sensitive mutants in Drosophila melanogaster: effects on premutational damage. | Drosophila melanogaster males from a Basc stock were mutagenized with either X-rays, ethyl methanesulfonate (EMS), or nitrogen mustard (HN2). Groups of identically treated males were crossed to different types of female. Sex-linked recessive lethals were scored as a genetic end point. The females used were homozygous for X-chromosomal mutations (mus(1)101D1, mus(1)104D1, mei-9 or mei-41D5) which lead to defective DNA repair and which increase the mutagen sensitivity of larvae. Females from a white stock with normal DNA repair capacities served as controls. The premutational lesions induced in mature sperm are only processed after insemination by the maternal enzyme systems present in the oocytes. Differences in the efficiency of the processing of lesions can lead to maternal effects on the frequency of mutations recovered from mutagenized sperm. It was found that, with the exception of mus(1)104D1, all mutants analysed significantly modify the mutation fixation of one or more types of premutational lesions. The most drastic effect is found with the mus(1)101D1 stock in which HN2-induced DNA cross-links do not lead to sex-linked recessive lethals. It is assumed that mus(1)101D1 is defective in an early step of DNA cross-link repair. Our first set of data clearly demonstrates that the study of maternal effects in Drosophila is an efficient tool to analyse the in vivo function of repair mutations on chemically induced mutagenesis. | 118,370 | [
0.154796,
0.02512215,
-0.2240373,
-0.222609,
0.05047039,
-0.02410574,
0.02734792,
-0.2180704,
0.1718094,
0.2050142,
-0.02706137,
0.241434,
0.07424692,
0.1765309,
-0.5161746,
-0.4709687,
-0.214352,
-0.0786277,
0.04934083,
0.269861,
0.4517484,
0.4043184,
-0.03351545,
-0.1... |
Mutagenic effectiveness of 14.1 MeV neutrons and 200 kV X-rays at the dumpy complex locus of Drosophila melanogaster. | The effectiveness of 14.1 MeV neutrons relative to 200 kV X-rays for the induction of the various kinds of dumpy mutation in mature sperm of Drosophila melanogaster was investigated. The estimated RBE values are: 0.52 for all complete mutations; 0.64 for the (olv, ov) types; 0.33 for the (ol, lv, o, v, c) types; 0.33 for all fractional mutations. These data lend support to the thesis that (1) complete dumpy mutations of the olv and ov types are more frequently associated with chromosomal aberrations than those of the ol, lv, o, v and c types, and (2) fractional mutations and complete mutations of the (ol, lv, o, v, c) types are most probably point mutational events. | 118,373 | [
-0.04640791,
0.1664069,
0.03936911,
0.02082369,
0.2181086,
-0.2666467,
0.2587252,
-0.3115289,
-0.1203747,
0.3520634,
0.0948109,
0.3830586,
-0.09336407,
0.1422218,
-0.9878294,
-0.3569076,
-0.5219084,
-0.02808115,
0.5985621,
0.07080512,
0.4074017,
0.6920438,
-0.1587121,
0... |
Effects of caffeine on maternal repair systems in Drosophila melanogaster. Concentration-dependent reversals of the effects of caffeine on chromosome loss and autosome-autosome translocations induced by x-rays in the paternal genome. | Drosophila melanogaster females were treated with 1% caffeine, mated with X-rayed males and the frequencies of induced sex-chromosome loss, translocations between the major autosomes and between the Y-chromosome and the major autosomes determined. In a reversal of the results obtained previously with 0.2% caffeine by Mendelson and Sobels, treatment of females with 1% caffeine led to a decrease in sex-chromosome loss, confirming preliminary data of Zimmering and Osgood and an increase in autosome--autosome translocations. It is suggested that the higher concentration of caffeine inhibits replication permitting more time available for chromosome-type restitutions by means of caffeine-insensitive repair mechanisms. In contrast with results for autosome--autosome translocation, the frequency of Y-autosome translocations was depressed below controls suggesting an isolation (by any one of several means) of Y-chromosome breaks from those in the autosomes. | 118,376 | [
0.2737231,
-0.07872917,
0.1318963,
-0.05746715,
0.2227681,
-0.1535121,
-0.1552435,
-0.3748443,
0.1377424,
0.0234007,
0.1130716,
-0.1596108,
0.04878585,
0.09214923,
-0.6573394,
0.1507755,
-0.3847642,
-0.01789848,
-0.02491514,
-0.06482547,
0.08821703,
-0.09298171,
-0.200938... |
Mutagenicity and metabolism of dimethylnitrosamine and benzo[alpha]pyrene in tissue homogenates from inbred Syrian hamsters treated with phenobarbital, 3-methylcholanthrene or polychlorinated biphenyls. | There are significant differences between mice and hamsters in polycyclic hydrocarbon and nitrosamine metabolism. Homogenates of liver, lung and intestinal mucosa from 6 strains of Syrian golden hamster were compared for their ability to metabolize benzo[alpha]pyrene (BP) and dimethylnitrosamine (DMN) to mutagens. Females of strains MHA/SSLak, LSH/SlLak, CB/SsLak, PD4/Lak LHC/Lak and Lak:LVG (SYR) were either untreated or received phenobarbital (PB), 3-methylcholanthrene (MC) or polychlorinated biphenyls (AR) to induce drug-metabolizing enzymes. Salmonella typhimurium TA92 and TA98 were used as indicators of the formation of mutagans. Dimethylnitrosamine demethylase (DMND) was assayed using 1 mM DMN as substrate. Aryl hydrocarbon hydroxylase (AHH) was measured using benzo[alpha]pyrene as substrate. MC does not induced AHH activity in hamster liver, but is an excellent inducer of enzymes converting BP to mutagens. This lack of correlation between increased AHH activity and increased metabolism of BP to mutagen in liver is in marked contrast to correlations seen in mice. MC induces AHH in hamster lung and intestinal mucosa. AR induces AHH in liver, lung and intestinal mucosa. Activity of DMND in liver is not affected by treatment of hamsters with BP or AR, but is repressed approx. 30% by treatment with MC. Activity of DMND and conversion of DMN to mutagen are correlated (r = 0.59) in hamster liver. Microsomes of hamster liver are more effective than those from mouse in converting DMN to mutagen, despite similar DMND activities in livers from the two species. | 118,380 | [
0.03104329,
-0.2203233,
-0.05279524,
-0.1596486,
0.04358598,
-0.0468353,
-0.3918436,
0.03234771,
0.2681798,
-0.2753123,
0.166852,
0.3646547,
0.1649113,
0.02583323,
-0.7664546,
-0.01981375,
-0.6825784,
0.00111329,
0.5404435,
0.3709066,
0.4952635,
0.194435,
-0.3218299,
-0... |
[Adrenergic activity and glycometabolic compensation in patients with diabetes mellitus]. | In an assessment of the degree of adrenergic activity in the course of diabetes mellitus, plasma levels and urinary excretion of norepinephrine and epinephrine were determined in 20 normal subjects and 47 diabetics: 11 in good control (group I), 23 in poor control (group II), 13 with frank ketoacidosis (group III). The study was repeated in groups II and III once good glycometabolic control had been achieved. Slightly above normal catecholamine levels were noted in group I, while there was a marked increase in group II. Group III shaved an enormous increase by comparison with the other two groups. After medical treatment values in group III fell to within the group I range. The conclusion is drawn that a close relationship exists between adrenergic acitivity and the degree of control of diabetes. The sympathetic nervous system, therefore, interferes in the course of diabetes with blood sugar control via numerous, complex mechanisms. | 118,410 | [
0.1145715,
0.1801038,
-0.2266881,
-0.1608581,
0.2579363,
-0.6818879,
-0.3019262,
0.02739861,
0.1160271,
-0.02719314,
-0.02489868,
-0.2553227,
0.05557807,
0.2237221,
-0.3835862,
-0.09773473,
-0.3684777,
-0.03156365,
0.1100755,
0.06160913,
0.05625124,
-0.1070391,
-0.0145120... |
[Unusual CT skull findings after preventive CNS treatment of acute leukemia in children]. | CT examinations of the cranium were carried out among 15 children who had previously received cranial radiation and i.th. methotrexate during leukemia management. Surprisingly, 10 of 15 patients had abnormal CT findings. Only 2 of the 10 "CT-positive" children were clinically-neurologically conspicuous. The visible CT alterations of brain structure seemed especially marked and frequent the more time had elapsed since CNS-treatment. The most striking CT pathologica were found among children up to 5 years of age, who were also conspicuous through convulsions and delayed development. | 118,413 | [
0.05867133,
0.1994544,
-0.005832288,
-0.323193,
0.188338,
-0.2497027,
-0.5625633,
-0.2763387,
-0.01254579,
0.05882088,
0.1783318,
0.4556672,
-0.1392494,
0.2059233,
-0.3983229,
0.03966963,
-0.2416432,
0.2292359,
-0.03287347,
0.3674954,
0.2313223,
0.2528285,
-0.0908803,
0... |
Sequences of large T1 ribonuclease-resistant oligoribonucleotides from protamine mRNA: the overall architecture of protamine mRNA. | Limited T1 ribonuclease digestion of the family of protamine mRNA's purified from rainbow trout testis yields several large oligoribonucleotide fragments ranging in size from 12--54 nucleotides in length. Several of these fragments purified by two dimensional gel electrophoresis contain several G residues and must represent nuclease-resistant, base-paired regions of the mRNA. Sequence analysis of these oligonucleotides by the method of Simoncsits, A., Brownlee, G.G., Brown, R.S., Rubin, J.R. and Guilley, H. (1977) Nature 269: 833-836, shows that these oligoribonucleotides arise from the 5'- and 3'-non-coding regions of the mRNA. Comparisons of the sequences of the large RNA fragment with DNA sequences obtained after cloning double-stranded protamine cDNA in the plasmids pBr322 and pmB9 show precise correspondence of a 54 nucleotide RNA fragment with positions 49--100 from the 3'-poly(A) tract and extending to within 5 nucleotides of the termination codon. Two other RNA fragments of 21 and 25 nucleotides in length arise from the 5'-non-coding region of the message and possess an AUG-sequence at their 3'-termini which is the initiation codon. The presence of distinct by homologous sequences in several sets of large RNA fragments is consistent with the presence of several closely related protamine mRNA's. | 118,437 | [
0.0515397,
0.138769,
-0.5944006,
-0.1719217,
0.08471964,
-0.1468136,
-0.01499922,
0.1174591,
0.3727562,
0.08275422,
0.03665677,
0.1122624,
0.1945913,
-0.1085557,
-0.1595897,
-0.2126406,
0.01956122,
-0.02423988,
0.2941875,
0.04154208,
0.3104438,
0.03496554,
-0.2066733,
-... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.