title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
|---|---|---|---|
Surgical treatment of endocardial cushion defects. | A recent surgical experience with the spectrum of atrioventricular (A-V) canal is reviewed. Twenty-five patients underwent surgery for the partial and complete from of this defect in the 4 years from 1971 through 1974. Sixteen had a partial defect, two a transitional defect and seven complete A-V canal. The characteristic murmurs accompanied by cardiac enlargement, pulmonay overcirculation and left axis deviation in the electrocardiogram were sufficient for diagnosis in most cases. Cardiac catherization was performed in all patients preoperatively and in 11 postoperatively. The operative approach, including a double patch modification of the usual repair for complete canal, is considered. Definitive repair, rather than pulmonary arterial banding, is advocated regardless of the patient's age. The operative mortality rate is low in patients with the ostium primum type of defect but is related to associated intracardiac anomalies in those with the complete form of the defect. Residual mitral insufficiency is a common finding after surgical repair of both partial (75 percent) and complete (100 percent) A-V canal. Although no patient in the series died of florid mitral regurgitation, the long-range effects of this complication may lead to mitral valve replacement. | 137,669 | [
-0.4727625,
0.0340096,
-0.5385082,
-0.1084343,
0.03176737,
-0.1769835,
-0.1822668,
0.01439655,
0.1848322,
0.1980843,
-0.07129666,
0.08098771,
-0.3223734,
0.07415293,
0.2694029,
-0.0782496,
-0.2633902,
0.1203586,
-0.1122258,
-0.1145746,
0.2167749,
0.3675371,
-0.0467815,
... |
Effect of increased pressures of oxygen, nitrogen, and helium on activity of a Na-K-Mg ATPase of beef brain. | Increased pressure of O2, N2,or He exert inhibitory or stimulatory effects on activity of Na-K-Mg ATPase obtained from beef brain cortex. Oxygen at 2 ATA and N2 at 4 ATA exert significant inhibitory effects whereas 3 & 4 ATA O2, 1-3 ATA N2 exert significant stimulatory effects. Effects of He on ATPase activity are qualitatively similar to those of N2. The effects of the gases are not qualitatively altered by the presence or absence of ATP. A pressure compensated analysis reveals that at the same pressures, O2 and N2 exert different effects of ATPase activity. | 137,712 | [
-0.05862072,
0.2160188,
-0.2254122,
0.1158234,
-0.04301712,
-0.03720018,
-0.1204105,
-0.220068,
0.06222509,
-0.2819299,
-0.07966425,
0.107713,
0.1103261,
-0.2854262,
-1.046809,
0.06440199,
-0.2867422,
-0.007817539,
-0.1913375,
-0.006764138,
0.2062993,
-0.03840357,
-0.0652... |
The primary inhibitor of plasmin in human plasma. | A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced. It contained antibodies agianst plasminogen+plasmin and an alpha2 protein. By crossed immunoelectrophoresis the alpha2 protein was shown to form a complex with plasmin, when generated by urokinase in plasma, and with purified plasmin. The alpha2 protein was eluted by Sephadex G-200 gel filtration with KD approx. 0.35, different from the other inhibitors of plasmin in plasma, and corresponding to an apparent relative molecular mass (Mr) of about 75000. By sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, the Mr of the complex was found to be approx. 130000. After reduction of the complex two main bands of protein were observed, with Mr, about 72000 and 66000, probably representing an acyl-enzyme complex of plasmin-light chain and inhibitor-heavy chain, and a plasmin-heavy chain. A weak band with Mr 9000 was possibly an inhibitor-light chain. The inhibitor was partially purified and used to titrate purified plasmin of known active-site concentration. The inhibitor bound plasmin rapidly and strongly. Assuming an equimolar combining ratio, the concentration of active inhibitor in normal human plasma was estimated to be 1.1 mumol/1. A fraction about 0.3 of the antigenic inhibitor protein appeared to be functionally inactive. In plasma, plasmin is primarily bound to the inhibitor. Only after its saturation does lysis of fibrinogen and fibrin occur and a complex between plasmin and alpha2 macroglobulin appear. | 137,718 | [
0.05323969,
0.1958302,
-0.2624232,
-0.2986899,
0.2990406,
0.1095281,
-0.04786409,
0.2163904,
0.1942672,
0.1928898,
-0.116357,
0.1825243,
0.3597697,
-0.3936644,
0.1148152,
0.147912,
-0.4544014,
0.1772538,
-0.06377949,
0.3383862,
0.3409025,
0.04809986,
-0.256362,
0.145809... |
The lipid requirement of the (Ca2+ + Mg2+)-ATPase in the human erythrocyte membrane, as studied by various highly purified phospholipases. | 1. When complete hydrolysis of glycerophosphlipids and sphingomyelin in the outer membrane leaflet is brought about by treatment of intact red blood cells with phospholipase A2 and sphingomyelinase C, the (Ca2+ + Mg2+)-ATPase activity is not affected. 2. Complete hydrolysis of sphingomyelin, by treatment of leaky ghosts with spingomyelinase C, does not lead to an inactivation of the (Ca2+ + Mg2+)-ATPase. 3. Treatment of ghosts with phospholipase A2 (from either procine pancreas of Naja naja venom), under conditions causing an essentially complete hydrolysis of the total glycerophospholipid fraction of the membrane, results in inactivation of the (Ca2+ + Mg2+)-ATPase by some 80--85%. The residual activity is lost when the produced lyso-compounds (and fatty acids) are removed by subsequent treatment of the ghosts with bovine serum albumin. 4. The degree of inactivation of the (Ca2+ + Mg2+)-ATPase, caused by treatment of ghosts with phospholipase C, is directly proportional to the percentage by which the glycerophospholipid fraction in the inner membrane layer is degraded. 5. After essentially complete inactivation of the (Ca2+ + Mg2+)-ATPase by treatment of ghosts with phospholipase C from Bacillus cereus, the enzyme is reactivated by the addition of any of the glycerophospholipids, phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine or lysophosphatidylcholine, but not by addition of sphingomyeline, free fatty acids or the detergent Triton X-100. 6. It is concluded that only the glycerophospholipids in the human erythrocyte membrane are involved in the maintenance of the (Ca2+ + Mg2+)-ATPase activity, and in particular that fraction of these phospholipids located in the inner half of the membrane. | 137,746 | [
0.08643608,
-0.3068993,
-0.1706179,
-0.1098105,
0.01666486,
-0.06025761,
0.187446,
0.1322042,
0.01436439,
-0.2338424,
0.1421843,
0.0516781,
-0.16475,
-0.1177043,
-0.5007307,
-0.1114762,
-0.3486685,
0.07106573,
0.1179245,
0.02921438,
0.6506419,
0.05870467,
-0.282838,
0.2... |
[IR-spectra of skeletal muscle mitochondria and endoplasmic reticulum]. | The existance of alpha- and beta-protein conformation in human straight abdomen muscle mitochondria and microsomal fraction was established. The stability of beta-conformation was provided by hydrophobic and lipid protein interactions. The band at 1730 cm-1, C=O stretching of the carboxyl groups, was more intensive in mitochondria, presented in microsomal fraction and disappeared after its isooctane treatment. From the reducing of NH, OH stretching frequency in microsomes films, was made a conclusion that hydrogen bonds were stronger in that membrane. | 137,753 | [
-0.08422807,
0.1298496,
0.08892198,
0.2220992,
0.1922553,
-0.03107725,
-0.2102927,
-0.01257639,
0.4178329,
-0.07199877,
0.06145084,
-0.3765943,
0.2697932,
0.06794116,
-0.6094995,
0.005081651,
-0.8849223,
-0.06979959,
-0.1971544,
0.3446384,
0.3816673,
0.2118781,
0.1516891,... |
Comparative physiologic dispositions of 5-fluoro-2'-deoxyuridine and 5-fluorouracil in mice bearing solid L1210 lymphocytic leukemia. | The physiologic disposition of 5-fluoro-2'-deoxyuridine (FUdR) and its metabolites, in particular the postulated active metabolite, 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP), has been studied in BDF1 mice bearing 6-day solid L1210 lymphocytic leukemia between 2 and 72 hours after a single iv dose of 14C-2-FUdR at 380 or 760 mg/kg (400-1300 microcuries/kg). FUdR itself was rapidly eliminated from tissues by urinary excretion and metabolism. However, its metabolites, including 5-fluorouracil (FU), persisted for 72 hours in the acid-soluble and -insoluble fractions of tissues. In tumor and small intestine, 60% of the 0- and 72-hour exposure to FdUMP, based on the area under the concentration-time curve, occurred between 24 and 72 hours after dosing. The results are compared with the physiologic disposition of FU after administration at 200 mg/kg iv which is an equimolar dose to FUdR at 380 mg/kg and approximately equitoxic to FUdR at 760 mg/kg. The molar concentrations of total drug equivalents, and of FU itself, after FUdR at 760 mg/kg were comparable to those after FU. However, the concentrations of FdUMP were lower than after FU, whereas the concentrations of drug equivalents incorporated into the acid-insoluble fraction, which includes equivalents incorporated into RNA, were generally higher than after FU. These data are correlated with the comparative antitumor effects and toxicity in mice of the fluorinated pyrimidines and with their clinical effectiveness. | 137,772 | [
-0.3693933,
0.1633347,
-0.2178766,
-0.06520322,
0.837413,
-0.40618,
0.07936735,
0.2097975,
0.06154722,
0.05248189,
0.01724741,
0.0002446212,
0.02744426,
0.1874637,
-0.3413359,
-0.410049,
-0.260987,
0.1885249,
-0.08476864,
0.3687724,
0.3373902,
0.2215983,
-0.1561861,
-0.... |
The haemodynamic effect of an intracranial arteriovenous anomaly. A Doppler-haematotachographic study. | Determination of the mean blood flow velocity by means of Doppler haematotachography is suggested as an aid in evaluating the haemodynamic changes associated with an intracranial arteriovenous anomaly. A Doppler haematotachogram (HTG) was obtained in 13 patients with a radiologically diagnosed arteriovenous anomaly, with marked interindividual variations in dimensions and blood supply; in 6 of these patients the Doppler HTG was obtained before and after total neurosurgical extirpation. The large majority of the patients showed a significant increase in diastolic, and to a lesser degree in systolic flow velocity at the level of the common carotid artery. In most cases the flow velocity curve of the ophthalmic artery showed a decrease in amplitude. These are the most useful parameters in evaluating the haemodynamic effect of an intracranial arteriovenous anomaly. After the surgical removal of the anomaly, the carotid flow velocity decreased significantly. In the internal and external jugular veins, Doppler-haematotachographic pulse waves were registered for the first time. These may have been conducted from the internal carotid artery to the jugular veins via the arteriovenous anomaly. The usefulness of this parameter is reduced because of the cumbersome calculations required to determine the time within which an arterial pulse wave conducted via the arteriovenous anomaly reaches the jugular vein. Registration of this unusual pulse wave is solely of theoretical value. | 137,793 | [
-0.04228564,
-0.0137241,
-0.5376727,
-0.4658543,
0.1789728,
-0.3776519,
-0.2494804,
-0.2545297,
-0.01246052,
0.1247571,
0.07843229,
0.09070936,
-0.4915693,
-0.5329707,
-0.2268919,
-0.01817404,
-0.1649687,
0.3245248,
0.06256304,
-0.1679613,
0.3410049,
0.09093188,
0.1508921... |
Steroid hormone formation in human ovarian follicles in vitro. | Ovarian follicles of 5 to 15 mm in diameter were isolated from 45 ovaries of 34 patients in the follicular and luteal phases of the cycle. Three experiments were done. In the first, follicles were minced and incubated in Krebs-Ringer bicarbonate buffer containing 1 to 2muCi of testosterone-4-14C in the presence or absence of 100 IU human chorionic gonadotropan (hCG). In the second, minced follicles were incubated with 100 muCi of sodium acetate-I-14C under identical conditions. In the third, ten follicles from a single patient in the late proliferative stage of endometrial dating were cut in halves and incubated with 100 muCi of acetate-I-14C under identical conditions. The minced follicle preparation was capable of aromatizing testosterone-4-14C into radioactive estrone and estradiol in significant amounts. Incorporation of radioactive acetate into pregenolone, progesterone, 17-hydroxypregnenolone, 17-hydroxyprogesterone, dehydroepiandrosterone, androstenedione, testosterone, estradiol and estrone was assessed by reverse dilution analysis with recrystallization to constant specific activity. The major radioactive products formed were androstenedione and 17-hydroxyprogesterone in the latter two experiments. Dehydroepiandrosterone was one of the major steroids in the second experiment. The minor products were testosterone, progesterone and pregnenolone. Smaller, but definite incorporations of radioactive acetate into estradiol and estrone occurred in the second experiment. On histological examination, the follicles were characterized by atretic changes. This distribution pattern of radioactive acetate among the steroids was considered to represent the steroidogenic profile of unstimulated or atretic follicles. | 137,808 | [
-0.2263833,
-0.09839073,
-0.2367335,
-0.02052127,
0.1997347,
-0.2964899,
-0.1717208,
0.09646074,
-0.1342379,
0.004310668,
0.04303342,
-0.03447697,
-0.04365731,
-0.05351838,
-0.1454126,
-0.05806596,
-0.01396519,
0.05110408,
0.1484349,
0.1560601,
0.1450621,
0.0267029,
0.067... |
[Mechanical activity of the papillary muscles of the rat heart under high altitude conditions]. | Mechanical activity of isolated papillary muscles in the left ventricle of rats was studied during permanent high altitude adaptation. The main computer parameters were: maximal developing tension, force of contraction, and capacity of papillary muscles at different periods of mountain adaptation (3, 15, 30 and 45 days). On the 15th day of mountain exposure, a statistically significant increase of maximal developing tension and papillary muscles activity was observed, which remained until the 45th day. Capacity was considerably increased on the 30th--45th day of adaptation. The data suggest positive effect of high altitude hypoxia on inotropic function of intact myocardium. | 137,822 | [
-0.1066911,
-0.1098773,
0.01516391,
0.104336,
-0.1849434,
-0.2210446,
-0.2811117,
0.1297702,
0.1205439,
0.0544363,
-0.08752111,
0.04934401,
-0.189654,
-0.5091514,
-0.3845103,
-0.260272,
-0.5178016,
0.2354521,
-0.1413104,
0.2023825,
0.2462648,
0.09938787,
-0.1719073,
0.0... |
Na+ transport in jejunal crypt cells. | As enterocytes migrate from crypts to villi they differentiate and mature. To examine the effect of epithelial differentiation on ion transport we studied 22Na+ efflux and (Na+--K+)-adenosine triphosphatase activity in suspensions of epithelial cells selectively isolated from different regions of the villus to compare crypt cells with villous tip cells. Enterocytes were isolated from rat jejunum by a dilation-vibration technique. Thymidine kinase, sucrase, and alkaline phosphatase activities were measured as markers of specific cell populations. Compared to villous cells, cells from the crypt region demonstrated lower (Na"--K+)-adenosine triphosphatase activity, lower total and passive Na+ efflux rate constants, and failure of Na+ transport to respond to an actively transported nonelectrolyte. | 137,828 | [
-0.1212173,
-0.3948248,
-0.251011,
-0.2660871,
0.2679494,
-0.4154857,
0.04806093,
0.0003791215,
0.2766809,
0.1116762,
0.1678486,
-0.1849381,
-0.224574,
0.1027795,
-0.5191457,
-0.09338053,
-0.9292284,
-0.02813035,
-0.2306988,
0.2513538,
0.2657005,
0.04416026,
-0.2336863,
... |
[The radiological diagnosis of Crohn's disease with special reference to involvement of the stomach (double-contrast method (author's transl)]. | The manifestations of Crohn's disease in the stomach can be demonstrated by a doublecontrast technique. Recognition of detailed mucosal changes, together with certain other typical findings, makes a diagnosis possible, as has been demonstrated by one case. | 137,847 | [
-0.1193334,
-0.3603102,
0.1484415,
-0.06876464,
0.1659317,
0.02480486,
-0.1155851,
-0.182605,
0.103346,
0.2784403,
0.1088355,
-0.05647258,
0.1035816,
-0.07046246,
-0.5811749,
-0.2246471,
-0.498065,
0.02572969,
0.06526005,
0.1821722,
0.1655154,
0.07616449,
-0.1151973,
0.... |
Reflex control of expiratory airflow and duration. | Unanesthetized, unrestrained cats were studied repeatedly after placement of a permanent tracheostomy, catheters for respiratory pressure measurements, and respiratory muscle EMG electrodes. The tracheostomy was opened or closed by a remote mechanism. Opening the tracheostomy reduced tracheal pressure to zero and diverted flow from the upper airway; closing the tracheostomy reestablished the normal pathway for airflow. Opening the tracheostomy during expiration evoked reflex responses in the diaphragm, in the laryngeal abductor and in abdominal expiratory muscles. These were sustained compensatory ("tracking") responses which, in each case, acted to offset loss of expiratory braking by the upper airway. Occlusion of the tracheostomy during expiration produced the opposite responses. Responses to tracheostomy opening usually overcompensated for the loss of upper airway resistance, suggesting that extrathoracic tracheal receptors may participate in tracking. Changes in the expiratory time course of lung volume were accompanied by changes in the duration of expiration. These "triggering" responses were shown to operate independently of the tracking responses though both were eliminated by vagotomy. | 137,890 | [
0.06743287,
-0.2437182,
-0.2055138,
-0.02434663,
0.1701356,
-0.299408,
0.02536715,
-0.3305583,
-0.1388658,
0.1830346,
0.4041893,
-0.252231,
0.03898991,
-0.2830515,
0.008274914,
0.08590315,
-0.7532229,
0.1444103,
-0.2530701,
-0.7319771,
-0.1419631,
-0.006319902,
0.1889485,... |
Purification and properties of the P15 specific restriction endonuclease from Escherichia coli. | The specific restriction endonuclease of the Escherichia coli plasmid, P15, has been purified to apparent homogeneity by a procedure that includes DNA-cellulose chromatography as well as a new endonuclease assay. Sedimentation on glycerol gradients showed two peaks of activity with values of 11.3 S and 15.7 S. The highly purified enzyme requires ATP and Mg2+ for activity and is stimulated by S-adenosylmethionine. A methylase activity is observed in the course of the endonucleolytic reaction which protects some of the DNA sites from cleavage. | 137,900 | [
-0.2633303,
0.08578005,
-0.1417472,
-0.2039613,
-0.1866467,
-0.065824,
-0.1150895,
-0.03079346,
0.122602,
0.1710664,
0.05513829,
-0.06095952,
-0.03616242,
0.1249309,
-0.6532965,
0.219189,
-0.2728749,
0.01887747,
-0.1007208,
0.3797455,
0.6907978,
-0.1560707,
-0.2142929,
... |
Transport of nutrients by a thermophilic bacterium--reconstruction of vesicles from crystalline ATPase or solubilized alanine carrier. | A strain of aerobic thermophilic bacteria was selected in order to purify highly stable membrane proteins and no reconstitute proteoliposomes capable of transporting nutrients from them. These proteins responsible for the transport could be divided into (1) proteins which supply energy to the transporting system, and (2) specific nutrient carriers driven by the energy. The former included a stable ATPase (TF1) and a lipoprotein TF0) which rendered TF1 sensitive to energy transfer inhibitors. The complex of TF0 anlysis of ATP. And one of the latter reported in this paper was alanine carrier protein which was driven by proton movement. TF1 was the first crystallized ATPase in biomembranes, and was reconstituted from its five different polypeptides, two of which were necessary for ATPase activity and four of which, for proton translocation. Purification of alanine carrier and reconstitution of proteoliposomes capable of alanine accumulation were also demonstrated. | 137,906 | [
-0.307158,
-0.08446637,
-0.2233848,
0.02235651,
-0.212613,
-0.2668404,
0.004692807,
0.1341969,
0.009651758,
-0.03749221,
0.1571067,
-0.1918748,
-0.190512,
-0.06312718,
-0.3077608,
-0.02279079,
-0.6215118,
-0.1498402,
-0.247689,
0.03881827,
0.08364113,
0.2192497,
-0.280691... |
Genetic alteration in the (Na+ + K+) ATPase transport system expressed in human lymphoblasts and their isolated plasma membranes. | A series of ouabain-resistant human lymphoblastoid lines were shown to be genetically altered in the (Na+ + K+) ATPase transport system. The sensitivity to ouabain of Rb+ uptake in intact cells and (Na+ + K+) dependent ATP hydrolysis in purified plasma membrane vesicles was compared with measurement of ouabain binding to intact cells. Preliminary evidence for at least two categories of ouabain resistance was obtained. | 137,909 | [
-0.1971044,
-0.09187734,
-0.2143824,
-0.09762769,
0.09632685,
-0.2241075,
-0.1428475,
0.1690738,
0.1688424,
0.4384708,
0.3924075,
0.2038346,
-0.08568344,
0.06202723,
-0.5229747,
0.04769444,
-0.4075764,
-0.06841271,
-0.1093836,
-0.02853561,
0.5466121,
0.123434,
0.001191724... |
HLA antigen studies in a family with C2 deficiency. | Two children in a family were found to be homozygous for C2 deficiency; both parents and a third child were heterozygous. C2 deficiency was associated with the HLA haplotypes carrying the antigens B18 and DW2. Antigen A10 was absent in this family. Mixed lymphocyte culture studies among the family members confirmed the association of C2 deficiency with the HLA-D locus. | 137,935 | [
-0.1226738,
-0.1658158,
0.06654175,
0.02204164,
0.3270704,
-0.1931193,
-0.3147013,
0.09489667,
0.1206027,
0.1396958,
0.1559862,
0.5117753,
-0.09494096,
0.02713893,
-0.2285374,
-0.2594464,
-0.3324312,
-0.06525503,
-0.2020088,
-0.1738761,
0.2080226,
0.2712182,
-0.2210839,
... |
Visual examination for blunt abdominal trauma. | Visualization of the peritoneal cavity to determine the presence of free blood is desirable to diagnose the condition of a patient who has suffered blunt abdominal trauma. Four-quadrant tap plus peritoneal lavage is reliable but the irrigant fluid may return only slightly pink or not return at all. In the Kansas University Medical Center emergency department, 26 Needlescopic examinations have been tried, with 92% accuracy and two false positive results. The Needlescope procedure is described. | 138,006 | [
-0.1193427,
0.2133646,
-0.5808992,
-0.3675205,
-0.01714356,
-0.09678577,
-0.4933291,
-0.0740433,
0.07506038,
-0.1067128,
0.4456479,
0.2456236,
-0.2469113,
-0.1308067,
-0.01996904,
-0.08599053,
-0.509665,
-0.1102412,
-0.0338846,
-0.6032956,
0.115132,
-0.2579281,
-0.1178622... |
[Status of physical environmental factors and the frequency of the chief complications of acute myocardial infarct]. | While studying the course of the disease in 2143 patients with macrofocal myocardial infarction it was found that with significant departure from mean for the city of Leningrad values of relative air humidity, intensity of terrestrial magnetism and differentials of atmospheric pressure the incidence of major complications of the disease is on the increase. An analysis of pertinent data leads to the conclusion on the need of arranging trial-tests to verify the effect on the course of acute myocardial infarction of the patient's stay in a specially equipped ward, where the parameters of the physical environmental factors are maintained at levels mean for a given locality. | 138,008 | [
-0.2342834,
-0.1367494,
-0.07327081,
0.2232358,
-0.2144769,
-0.2252421,
-0.1884616,
-0.1502765,
-0.1436091,
-0.1473443,
-0.03487175,
-0.2721775,
0.07553176,
-0.3239876,
-0.202333,
-0.02509586,
-0.06930194,
-0.03310618,
-0.2950122,
0.1653698,
0.1107806,
-0.05007359,
-0.009... |
[Damage of the cochlea and of the cochlear nucleus after the application of aminoglycosid antibiotics--a comparative light-, transmission- and scanning electronmicroscopic study in the guinea pig (author's transl)]. | Ototoxic alterations were studied in the guinea pig organ of Corti and in the cochlear nucleus after high administrations of aminoglycosid antibiotics (Gentamicin/Tobramycin, 150 mg/kg body weight/day or Amikacin 300 mg/kg body weight for 10 days). After survival times up to 22 days the animals were examined. By means of morphology degeneration was found in the cochlear nucleus before we could state it in the organ of Corti. After longer survival times, when we observed heavy degeneration in the organ of Corti we found only a few myelin figures of axons in the cochlear nerve. Cochlear nucleus: As an early sign of degeneration alterations in the mitochondria were found. Compared with the organ of Corti the damage was less widespread in the second order neurons of the afferent auditory pathways, their dendrites or nerve endings. Quantitative calculations however, were not performed. In axosomatic synapses we could observe a way of degeneration which was not reported before in damage caused by aminoglycosid antibiotics. Free postsynaptic densities were observed to be invaginated into the neuron as a probable way of sequestration. As well we could observe reoccupation of the synaptic sites by a probable sliding of neighbouring nerve endings into the free intracellular space. (Similar observations were reported first by Gentschev and Sotelo [1973] after ablation of the cochlear nerve in rats. | 138,049 | [
0.03108445,
-0.1215817,
-0.04660809,
0.2203569,
0.1228459,
-0.4678977,
-0.0280206,
-0.05904408,
0.08649018,
-0.1767173,
0.03267657,
-0.01049128,
0.07138835,
0.04775218,
-0.1113166,
0.001526234,
-0.5584728,
0.1173863,
-0.1262933,
-0.01111011,
0.4081081,
0.01362879,
0.22396... |
[Otosclerosis involving the capsule and inner ear. Morphological findings (author's transl)]. | The problem of otosclerotic inner ear damage is considered on the basis of morphological findings. It is pointed out that the disease of the otic capsule certainly also affects the underlying sense organ. This arises through disturbance of the perilymphatic electrolytes, venous stasis and thus changes in the circulation, disturbed regulation of endolymph and, more rarely, the direct effect of the focus on the auditory nerve or calcification of the mobile membranes. | 138,051 | [
-0.4169317,
-0.02311796,
0.09577652,
0.008739703,
-0.1359137,
-0.4421553,
-0.1641146,
0.01990914,
0.0233642,
0.100805,
0.09496838,
0.01024444,
-0.1039011,
-0.1344961,
-0.5850692,
-0.001980716,
-0.2632319,
0.1195926,
-0.117677,
0.1067323,
0.5095016,
-0.05421848,
-0.0645723... |
[Zinc treatment of acrodermatitis enteropathica (author's transl)]. | Treatment of acrodermatitis enteropathica with zinc sulfate, first described by Moynahan and Barnes in 1973, has been widely accepted. We have treated a case of acrodermatitis enteropathica successfully with this drug. The 14-year old boy had to discontinue clioquinol because of a partial opticus atrophy. The skin lesions deteriorated acutely. Following zinc sulfate treatment the lesions rapidly disappeared and the general health of the patient greatly improved. Small doses of zinc sulfate are sufficient for maintenance therapy. The pathogenesis of acrodermatitis enteropathica is reviewed and the modes of action of zinc therapy are considered. | 138,080 | [
-0.09746023,
-0.2557229,
-0.0467031,
-0.2390577,
0.4504014,
-0.1885683,
-0.2462045,
0.2303274,
-0.01991734,
-0.09026725,
0.2395903,
-0.03063246,
0.3824013,
0.1416763,
-0.4218585,
0.007826938,
-0.2450388,
-0.1086476,
0.07268384,
0.02153921,
0.3791427,
0.0009704864,
-0.0540... |
[Origin and significance of perioral dermatitis (author's transl)]. | We report on 888 patients with perioral dermatitis treated in our department from 1969-1975. The patients are evaluated according to age, sex and duration of treatment with fluorinated corticosteroids. The enormous increase of morbidity and the importance of highly effective topical corticosteroids are emphasized. | 138,081 | [
0.06318704,
-0.3043336,
-0.02134314,
0.1214415,
0.02151973,
-0.1490267,
-0.07309684,
-0.01161338,
-0.233283,
-0.002772965,
0.05044888,
-0.1374718,
0.2800352,
0.2524115,
-0.2882397,
-0.4576301,
0.0912847,
0.2746637,
-0.1380031,
0.5676396,
0.2207534,
0.2762987,
-0.1502941,
... |
Glycolysis and glycogen metabolism after inhibition of hexose monophosphate pathway in C6-GLIAL cells. | 1. 6-Aminonicotinamide (0.01 mg/ml) leads to a strong accumulation of 6-PG in C6 glial cells after 24h. 2. The accumulated 6-PG is dephosphorylated to gluconate which easily permeates the cell membrane. Extracellular gluconate is formed at a rate of 12% of the total glucose consumption. 3. 6-PG as competitive inhibitor of the PG1 caused a reduction of the glycolytic flux of about 40%. 4. The reduced glycolytic flux lowers the ATP concentration under anaerobic conditions to 75% of the controls. 5. The glycogen content after 6-AN is increased by 50%, probably by the activation of the glycogen synthetase due to the higher Glc 6-P concentration. 6. The fibroblast-like morphology of the C6 cell line has typically changed under 6-aminonicotinamide. | 138,094 | [
-0.2089745,
-0.1139078,
-0.3188863,
-0.160936,
0.1330193,
-0.0402049,
-0.02468118,
0.01161535,
-0.3601456,
0.1553213,
-0.1550135,
0.2420331,
-0.2656942,
0.001880736,
-0.4308832,
0.1156721,
-0.2919641,
0.4389929,
-0.216063,
0.1920677,
0.4912435,
0.3522204,
0.02365714,
0.... |
Differential effects of harmaline and ouabain on intestinal sodium, phenylalanine and beta-methyl-glucoside transport. | Harmaline inhibits both the Na+ -K+ -ATPase activity and the uptake of L-phenylalanine in guinea-pig intestinal mucosa. The latter effect is not a direct consequence of the former, since higher concentrations are needed to inhibit the enzyme than the influx into the mucosa; Furthermore the uptake is still sensitive to harmaline when the Na+ -K+ -ATPase has been fully inhibited by ouabain. Harmaline can inhibit L-phenylalanine influx at a concentration at which it does not affect intracellular ion concentrations. Ouabain, however, inhibits the uptake of L-phenylalanine only after a 30 min preincubation period, when the intracellular sodium concentration reached the extracellular level. Harmaline also interferes with the influx of beta-methyl-D-glucoside in the mucosa of the dog colon. Addition of harmaline at the mucosal face of the tissue suppresses all net transport of sodium and chloride ions and L-phenylalanine across the mucosa. Thus the same mode of action appears to apply in both the guinea-pig ileum and the dog colon. | 138,095 | [
-0.2324926,
0.03750719,
-0.2154794,
0.09413037,
0.1921821,
-0.2093903,
0.01923034,
-0.03845416,
-0.1067802,
0.2014341,
0.1100386,
0.1006706,
0.1541947,
0.1181965,
-0.4087971,
0.08047663,
-0.5240208,
0.1148613,
0.02149016,
-0.2169325,
0.2784752,
-0.05284617,
-0.1784183,
... |
The family in physical therapy. | Family Focus is a training program implemented by the Division of Physical Therapy at Stanford University in cooperation with the Mental Research Institute of Palo Alto, California. Through this program, inpatients at the Stanford University Hospital and their families have the opportunity to live in a modular home on the hospital grounds prior to discharge and receive training in the activities they will need to carry out at home. Psychologists consult with the physical therapists on ways to help the family cope with stressful situations. The advantages of including the entire family in health care planning and treatment are described and suggestions are made for applying the principles in any physical therapy setting. | 138,143 | [
-0.33156,
0.2008532,
-0.01834217,
0.255566,
0.3092096,
-0.1451956,
0.02607177,
-0.1528144,
-0.0004541217,
0.0140537,
0.0128074,
-0.4683085,
-0.2721372,
-0.135553,
-0.1224204,
0.09437677,
0.04097825,
0.1414908,
-0.108327,
0.03920899,
-0.2569324,
-0.240388,
-0.1771045,
-0... |
[Clinical course of congestive cardiomyopathy]. | In 30 patients with the clinical, hemodynamic and angiographic findings of congestive cardiomyopathy, physical working capacity (PWC) and left ventricular (LV) ejection phase indexes at rest (EI), assessed by LV ejection fraction, enddiastolic volume, and mean rate of circumferential fiber shortening were determined. Based on PWC and EI, the following 3 functional groups were obtained: group 1 (n = 6) with normal PWC and borderline EI (subclinical congestive cardiomyopathy), group 2 (n = 10) with normal PWC and decreased EI, and group 3 (n = 14) with reduced PWC and severely depressed EI. The clinical course was followed for 24 months. In group 1 the functional state remained unchanged in all 6 patients. In group 2, 3 patients, changed to functional group 3 and 2 died during the observation period. In group 3, 3 patients improved and changed to functional group 2, 5 remained unchanged and 6 died after an observation period of 16 months. It is concluded (1) that PWC in congestive cardiomyopathy may be preserved despite depressed LV function, and (2) that the functional classification has important prognostic implications, in view of the fact that annual mortality in group 1 was 0%, in group 2 7% and in group 3 32%. | 138,185 | [
-0.1444273,
0.29469,
0.0913396,
-0.4029365,
0.2739963,
-0.4292005,
0.2391408,
-0.06149979,
-0.3677679,
0.2848151,
-0.3000013,
0.3505979,
0.001265933,
0.2380453,
-0.5104489,
-0.3978809,
-0.1143109,
0.4888947,
0.3559083,
0.2232435,
0.05744067,
0.2401455,
-0.1173356,
0.044... |
[Toxic reactions to gold salts with severe enterocolitis in a patient with rheumatoid arthritis]. | A case of severe enterocolitis which occurred on initiation of chrysotherapy for rheumatoid arthritis is reported. The patient, a male aged 50 years, suffered from dramatic diarrhea for several weeks. Biopsy specimens from the stomach, ileum and colon revealed signs of inflammation. Later, general toxicodermia, mouth ulcers, blood eosinophilia and asymptomatic proteinuria were observed. After several months all the side effects disappeared. The toxicodermia was the most persistent. | 138,187 | [
-0.09162267,
-0.04400625,
0.007572128,
0.07913276,
0.325527,
-0.01274879,
-0.1906235,
-0.2723583,
-0.0698303,
0.1043075,
0.1074342,
0.04329449,
0.1446065,
-0.1699429,
-0.26989,
0.1186073,
-0.4020213,
0.3377337,
0.1207182,
-0.1382114,
0.1078347,
0.27764,
0.007570257,
-0.... |
Inhibition of the mixed leucocyte reaction by histocompatibility antibodies requires the Fc part. | We have examined the effect in mixed leucocyte culture (MLC) with rat cells of the IgG fraction of alloantibodies against the major histocompatibility complex (anti-BN major) and against some of the minor histocompatibility antigens. Anti-BN major specifically inhibited the MLC response, both when BN cells were responding and stimulating cells. Absorption with BN erythrocytes completely removed the haemagglutinating antibodies but had no effect of MLC inhibition, while absorption with BN lymphocytes removed MLC inhibition as well as haemagglutinating antibodies. The F(ab')2 fragments of anti-BN major did not inhibit MLC, although they still had the capacity to combine specifically with BN lymphocytes. We therefore suggest that the inhibition of MLC responses by alloantibodies is either because of a cytotoxic effect (even if cytotoxicity could not be demonstrated in unmixed cultures) or a blocking of antibody-coated lymphocytes by Fc receptor-bearing cells. Antibodies against minor histocompatibility antigens did not inhibit MLC. | 138,227 | [
-0.038173,
-0.1330254,
-0.0558343,
0.03004002,
0.3056395,
-0.02464852,
-0.1378185,
0.217033,
0.000543358,
0.1531238,
0.09922252,
0.4086579,
-0.03289868,
0.09008538,
-0.4246734,
-0.1199073,
-0.5017571,
0.1406233,
0.04900974,
0.2495686,
0.4326122,
0.1394184,
-0.1086499,
0... |
[Organization and work experience of the endoscopy office in an emergency abdominal surgery clinic]. | Within a five-year period in a special room intended for endoscopy 1934 investigations were performed, including 1318-on urgent indications. 77 esophago-gastroduodenoscopies were performed elsewhere-in the operating room, in the intensive care unit; 35 investigations were carried out under general anesthesia with the use of myorelaxants of a short effect. In 256 cases the neccesity of active infusion therapy and reanimation arose. In the paper, the authors' considerations concerning the need of organization, in large cities, of a mobile endoscopic team attached to the emergency medical aid are given with special reference to some features of the organization of endoscopy room in the clinic of emergency abdominal surgery. | 138,241 | [
0.003886195,
-0.1073991,
-0.1543319,
-0.163411,
0.1338759,
-0.04062549,
-0.2253625,
-0.2921502,
-0.1457504,
0.2132586,
0.03530037,
-0.3348946,
0.113836,
-0.1877453,
-0.3161465,
0.1389329,
-0.4438079,
0.1550698,
-0.02734128,
0.1882717,
0.2671639,
0.05489641,
-0.002367337,
... |
[Influence of DCMU on the formation of lipids and fatty acids, and on the ultrastructure of Euglena gracilis (author's transl)]. | Euglena gracilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Subsequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-1,1 dimethyl urea (DCMU) (1.7X10(-9)--1.0X10(-5)mol/l) added to the nutrient medium. Under non-photosynthetic conditions (addition of 10(-6)--10(-5)mol DCMU/l), the cells still produced chloroplast structures and a considerable percentage of compounds typical for chloroplasts, such as galactolipids (40-50%), chlorophylls (40%), and polyunsaturated C16 and C18 fatty acids (16:2, 16:3, 16:4, 18:2, 18:3) (65%). The results indicate that under these conditions the above compounds are synthesized from carbon sources outside the chloroplasts. | 138,287 | [
0.07296062,
-0.001350919,
0.2317523,
0.01499128,
0.3242756,
-0.2192301,
-0.09440046,
0.09987004,
0.06561607,
0.03004256,
0.00213965,
0.291675,
-0.1525636,
0.1009573,
-0.8199114,
-0.1400683,
-0.3899894,
0.5318013,
-0.1945677,
0.4477819,
0.6167668,
0.4121566,
-0.1131984,
... |
Labeling of animal cells with fluorescent dansyl cerebroside. | A dansyl (diaminoaphthalenesulfonyl)-derivative of cerebroside was prepared which could be effectively incorporated into the plasma membranes of tissue culture cells and erythrocytes. The cells which had assimilated the glycolipid fluoreced intensely and could be observed under a fluorescent microscope. Cells were initially labeled rather homogeneously over the whole surface. With longer incubation time organization of the fluorescent glycolipid took place and patches of the lipid in the membrane were formed. The redistribution and organization of the membrane lipid could be demonstrated most clearly when cells labeled with this fluorescent glycolipid were infected with myxoviruses. After infection of MDBK and BHK cells with fowl plaque virus areas of dense fluorescence appeared at margines of neighboring cells. When BHK cells were infected with Newcastle disease virus fusion of the cells was accompanied by complete redistribution of the glycolipid. Erythrocytes could also easily incorporate dansyl cerebroside. Chicken erythrocytes which contain cytoplsmic and nuclear membranes incorporated the fluorescent glycolipid in both membranes. | 138,295 | [
-0.0002605013,
-0.1177253,
-0.4374162,
0.06627952,
-0.003393494,
-0.1157119,
0.02401158,
0.1282379,
-0.222408,
-0.02110697,
0.02144487,
0.2235011,
-0.08679284,
-0.03527756,
-0.130658,
0.03416014,
-0.1960167,
0.2275476,
-0.1722357,
0.3121571,
0.3460121,
0.087413,
-0.196816... |
Characterization of the glycolysis pathway and the plasma membrane of Lactobacillus and Bifidobacterium strains. | The characteristics of the glycolytic pathway and the plasma membrane of Lactobacillus and Bifidobacterium were studied. The enzyme system of glycolysis (hexokinase, glucokinase and pyruvate kinase) which is the main source of energy in the anaerobic condition was localized in the cell soluble fraction (cytoplasma) of all species examined. Neither electron transfer chain components nor oxidase activities were found in anaerobically cultured Lactobacillus and Bifidobacterium. Adenosine triphosphatase (ATPase) activities were mainly localized in the plasma membrane, suggesting that membrane ATPase is playing a key role in membrane transport and ATP synthesis of anaerobic bacilla. SDS-polyacrylamide gel electrophoresis of membranes showed remarkable differences between the polypeptides patterns of B. adolescentis and B. bifidum. Such peculiarities in polypeptide patterns among the same genus may be useful in the identification of species. | 138,331 | [
-0.06595685,
-0.08675206,
0.1645986,
0.3336917,
0.05927088,
-0.3945357,
-0.2431176,
0.08292815,
-0.1294651,
0.0003654082,
0.2707591,
0.06698782,
-0.1070376,
0.4460841,
-0.1939684,
-0.2209651,
-0.4732927,
-0.08669512,
-0.06931347,
-0.5360612,
0.6917214,
0.4492977,
-0.09321... |
Fetal pancreatic glucagon responses in glucose-intolerant nonhuman primate pregnancy. | Rhesus monkey pancreatic alpha-cell function in streptozotoc-induced glucose-intolerant pregnancy is similar to that in normal primate pregnancy. Specifically, basal maternal and fetal plasma glucagon levels equate, and the fetal alpha cell does not respond to the glucagonogenic stimulus of either intravenous alanine or insulin-induced hypoglycemia. This contrasts with the accelerated maturation of the fetal beta cell in glucose-intolerant pregnancy, and does not support the concept of functional coupling of the pancreatic islet by a common glucose-based process. Fetal plasma glucagon levels do increase after L-dopa injection to the fetus. These data indicate that alpha cell unresponsiveness is a function of the glucagon-releasing mechanism rather than inadequate hormonal synthesis. | 138,366 | [
-0.0382676,
-0.287843,
-0.4591505,
0.01823498,
0.4436797,
-0.3391848,
-0.2193414,
-0.04396531,
0.3193678,
0.2998974,
0.1120892,
-0.03698325,
-0.1244702,
0.06601301,
-0.09009584,
-0.38127,
-0.2093579,
0.2399873,
-0.1498464,
0.02952125,
0.1675792,
0.03594537,
0.08288823,
... |
The value of pre-therapy peritoneoscopy in localized ovarian cancer. | Because of the recent reports indicating the high incidence of unsuspected diaphragmatic metastases in presumed localized ovarian cancer, we have carried out peritoneoscopy on 14 consecutive patients referred with the diagnosis of Stage I or II ovarian cancer. Of the 14 women none was demonstrated to have diaphragmatic metastases, but 35 per cent were found to have cytologic washings demonstrating malignant cells. The significance of these findings is discussed. | 138,367 | [
0.01424363,
-0.2456647,
-0.3477673,
-0.1443811,
-0.1566108,
-0.2926393,
-0.08464763,
-0.3915731,
-0.1267356,
-0.01672954,
0.2173045,
-0.02621353,
0.0803368,
-0.2816158,
-0.3687114,
-0.2686157,
-0.3141354,
0.2068983,
-0.0291284,
-0.1358282,
0.2812109,
0.1306764,
-0.0749042... |
Immunologic approach to the treatment and prevention of gastrointestinal ulcers. | Reported clinical correlations between gastroduodenal ulcer and allergies point up the Arthus ulcer as a possible laboratory model for gastroduodenal ulcer. Successful immunological containment of gastroduodenal ulcer was achieved by utilizing Arthus ulcer dynamics in 12 patients over a two and one-half year period. | 138,370 | [
-0.1058798,
-0.02846866,
-0.1480909,
-0.1105843,
0.2350256,
-0.2092398,
-0.1905121,
0.02508604,
0.2856646,
-0.01480686,
0.09916241,
-0.3195757,
0.182237,
0.02668103,
-0.3255679,
0.3403,
0.07826509,
0.1094733,
-0.2478822,
0.5213274,
-0.06519046,
-0.008781436,
-0.02914527,
... |
[The immunological mechanisms of psoriasis]. | The occurrence of ANA in eluates of lymphocytes and polymorphonuclear leucocytes obtained in five untreated psoriasis patients could be demonstrated by means of the indirect immunofloruescence technique. These antibodies appear to be mainly directed against the nuclei of the basal cell layer, however, ANA directed against the nuclei of epidermal and dermal cells have also been encountered. The immunological mechanisms in psoriasis may therefore be looked upon as the result of an alteration in the structure of nucleic acid or nuclear protein of the basal layer cells. Finally psoriasis may be the result of an interplay of various exogenous factors and a special genetical make-up leading to the release of certain nuclear proteins initiating a delayed type immune response at the onset and an Arthus type reaction located in the upper layers of the epidermis at a later stage of the disease, and as a secundary phenomenon the derailment of keratinization. Moreover a slightly decreased mean percentage of circulating lymphocytes forming rosettes with sheep erythrocytes have been found in the patients studied. This slight decrease suggests a diminution of the T-lymphocyte control facilitating the antibody production by B-lymphocytes. The course of events in psoriasis is a self perpetuating inflammatory process. | 138,379 | [
0.01896211,
-0.1431125,
0.01168683,
0.2486003,
0.3585502,
-0.5422251,
-0.006882782,
-0.1064951,
0.2047553,
0.1645462,
-0.2769802,
-0.2009441,
0.3574176,
-0.03748192,
-0.117756,
-0.1135289,
-0.1989495,
0.03366734,
-0.06297957,
0.203164,
0.5277767,
0.3203009,
-0.1238157,
... |
Liberation of protoplasts from mycelium of Neurospora crassa by means of enzymes obtained from autolyzed cultures of this fungus. | Spherical protoplast-like structures were released from Neuropsora crassa mycelia under the influence of cell-wall-lytic enzymes obtained from autolyzed cultures of this fungus, in 50 mM borate-citrate-phosphate buffer, pH 5.5 with 0.5-0.8 M mannitol for appropriate osmotic pressure. The protoplasts retain their morphological aspect for several days at 4 C, or for a longer time when they are washed by centrifugation and incubated with 0.8 M mannitol. The free protoplasts are sensitive to osmotic shock and lyse immediately when placed in distilled water; the vacuoles and other cytoplasmatic material remain intact for some time. | 138,385 | [
-0.0231655,
-0.3672149,
0.1049136,
0.01014723,
-0.2236981,
-0.2252531,
-0.1201378,
-0.2904792,
0.2194279,
0.06523094,
0.3544782,
-0.2831086,
-0.1825677,
0.01628862,
-0.2925117,
0.2543801,
-0.3454792,
0.03054375,
0.03493614,
0.04805183,
0.6809559,
0.216693,
-0.1256612,
-... |
Congenital intracardiac band: a rare cause of nonrheumatic combined aortic and mitral regurgitation. | A patient had a congenital intracardiac band lying across the aortic valve that caused combined aortic and mitral valvular regurgitation. The band was excised, and the damaged aortic valve was replaced with a prosthesis. The case illustrates that not all congenital intracardiac bands are completely asymptomatic and not all bivalvular incompetence is rheumatic in origin. | 138,407 | [
-0.4178272,
0.1358341,
-0.2041463,
0.02614665,
-0.04027406,
-0.1290014,
-0.2980426,
-0.05221773,
0.1426588,
0.07039964,
0.2693333,
0.400355,
0.04992202,
-0.3132482,
0.1304912,
0.07845514,
-0.7071257,
0.045818,
-0.1052897,
-0.1227896,
0.1410096,
0.1015082,
0.03461161,
0.... |
[Comparative pathology of the choroid vessels in glaucoma, hypertension and arteriosclerosis]. | Results of histological and histochemical studies of the choroid of the eyes of deceased old people who when alive suffered from glaucoma, hypertensive disease and atherosclerosis, are presented. It was established that hypertensive disease, general atherosclerosis, symptomatic hypertension brought about peculiar changes in the choroid vessels and stroma. There were revealed changes in the venous system of the choroid of the glaucomatous eyes, which testified to the presence of venous congestion in them, this apparently being one of pathogenetic factors in origination of primary glaucoma. | 138,410 | [
0.122316,
0.07309143,
0.05512651,
0.1811229,
0.1048251,
-0.3112963,
0.06008684,
0.03731469,
0.03450324,
0.06346041,
0.09225032,
0.0478855,
-0.06076605,
-0.2968164,
-0.6333745,
-0.1567612,
-0.1599865,
0.3831451,
0.07499126,
0.4954972,
0.09285662,
0.08723547,
-0.1510538,
... |
Transient phase of adenosine triphosphate hydrolysis by myosin, heavy meromyosin, and subfragment 1. | The transient phase of adenosine triphosphate (ATP) hydrolysis (early burst) was investigated for myosin, heavy meromyosin (HMM), and subfragment 1 (S-1) over a range of temperatures and pH's. The burst size at pH 8,20 degrees C, is 0.8-0.85, based on steady-state and transient measurements. The equilibrium constant for the enzyme-substrate to enzyme-product transition is 0.85 +/- 0.05. It is concluded that both myosin heads undergo the rapid hydrolysis step and that there are no significant differences for S-1 vs. HMM or myosin. The transient data are fitted reasonably well by a single rate process, but available evidence is consistent with some heterogeneity and a range of rate constants differing by a factor of two. At pH 6.9 and 3 degrees C, the burst size is 0.5 and the hydrolysis is slower than the configuration change measured by fluorescence. The results are consistent with the kinetic scheme (see article). The lower burst at low temperature and pH can be partly explained by a reduction in the equilibrium constant, K3, and ATP can be synthesized on the enzyme by a pH-temperature jump. | 138,438 | [
-0.1556154,
-0.1300616,
-0.5763829,
-0.3439298,
0.1117842,
-0.3268723,
0.0001461012,
0.1769952,
-0.009186122,
0.01343269,
0.08358122,
0.1720218,
0.08731801,
0.1381849,
-0.5552374,
-0.009965387,
-0.5797595,
-0.0389158,
0.2306687,
0.3426295,
0.8091628,
-0.1525184,
-0.070889... |
Energy transduction in Escherichia coli. The effect of chaotropic agents on energy coupling in everted membrane vesicles from aerobic and anaerobic cultures. | 1. The transduction of energy from the oxidation of substrates by the electron transport chain or from the hydrolysis of ATP by the Mg2+-ATPase was measured in everted membrane vesicles of Escherichia coli using the energy-dependent quenching of quinacrine fluorescence and the active transport of calcium. 2. Treatment of everted membranes derived from a wild-type strain with the chaotropic agents guanidine-HC1 and urea caused a loss of energy-linked functions and an increase in the permeability of the membrane to protons, as measured by the loss of respiratory-linked proton uptake. 3. The coupling of energy to the quenching of quinacrine fluorescence and calcium transport could be restored by treatment of the membranes with N,N'-dicyclohyexylcarbodiimide. 4. Chaotrope-treated membranes were found to lack Mg2+-ATPase activity. Binding of crude soluble Mg2+-ATPase to treated membranes restored energy-linked functions. 5. Membranes prepared from a wild-type strain grown under anaerobic conditions in the presence of nitrate retained respiration-linked quenching of quinacrine fluorescence and active transport of calcium after treatment with chaotropic agents. 6. Everted membrane vesicles prepared from an Mg2+-ATPase deficient strain lacked respiratory-driven functions when the cells were grown aerobically but were not distinguishable from membranes of the wild-type when both were grown under anaerobic conditions in the presence of nitrate. 7. It is concluded (a) that chaotropic agents solubilize a portion of the Mg2+-ATPase, causing an increase in the permeability of the membrane to protons and (b) that growth under anaerobic conditions in the presence of nitrate prevents the increase in proton permeability caused by genetic or chemical removal of the catalytic portion of the Mg2+-ATPase. | 138,439 | [
-0.5717235,
0.03421732,
-0.4306537,
0.1371782,
-0.09538393,
-0.2555269,
0.06016051,
-0.1203848,
0.07580897,
0.01862764,
-0.07852712,
0.3132764,
-0.1642498,
0.1592737,
-0.6579552,
0.235773,
-0.5883555,
-0.1231782,
-0.2502841,
-0.1065908,
0.2280964,
0.2253386,
-0.1071241,
... |
Variation in sensitivity of the cardiac glycoside receptor characteristics of (Na+ + K+)-ATPase to lipolysis and temperature. | 1. The rate of binding of [3H]ouabain to untreated membrane preparations of [Na+ +K+]-ATPase is a timperature--dependent process displaying a thermal transition close to 25degreesC. The apparent energies of activation which can be calculated above and below this transition are similar to, but not identical with, those previously reported for activation of the enzyme by cations. 2. Treatment of the enzyme preparation with detergents or lipolysis with phospholipase A eliminates the thermal transition resulting in linear Arrhenius plots. 3. The number of sites available for [3H]ouabain binding is not temperature dependent as the amount of [3H]ouabain bound at equillbrium is not changed between 10 and 37 degrees C. 4. Treatment of the enzyme with phospholipase A results in time-dependent changes in the number of binding sites for [3H]ouabain at equilibrium. 5. Treatment of the membrane enzyme preparations with detergents reveals additional [3H]ouabain binding sites which are extremely sensitive to lipolysis with phospholipase A. 6. There are a number of [3H]ouabain binding sites which remain resistant to lipolysis by phospholipase A in either untreated or detergent-treated membrane preparations. 7. It is suggested that [3H]ouabain binding sites exist in the membrane in at least two different environments, one of which is resistant the other sensitive to attack by phopholipase A. | 138,443 | [
-0.2005787,
-0.263349,
-0.5369153,
-0.05392807,
0.001768722,
-0.20122,
-0.1190632,
0.1013234,
0.3180566,
0.1765124,
0.06037696,
0.1335027,
0.02573694,
-0.08861366,
-0.2725006,
0.09101511,
-0.5883179,
0.03160832,
-0.1248807,
0.2300572,
0.3218421,
0.2699224,
0.006236138,
... |
On the role of enzyme cooperativity in metabolic oscillations: analysis of the Hill coefficient in a model for glycolytic periodicities. | The role of enzyme cooperativity in the mechanism of metabolic oscillations is analyzed in a concerted allosteric model for the phosphofructokinase reaction. This model of a dimer enzyme activated by the reaction product accounts quantitatively for glycolytic periodicities observed in yeast and muscle. The Hill coefficient characteristic of enzyme-substrate interactions is determined in the model, both at the steady state and in the course of sustained oscillations. Positive cooperativity is a prerequisite for periodic behavior. A necessary condition for oscillation in a dimer K system is a Hill coefficient larger than 1.6 at the unstable stationary state. The analysis suggests that positive as well as negative effectors of phosphofructokinase inhibit glycolytic oscillations by inducing a decrease in enzyme cooperativity. The results are discussed with respect to glycolytic and other metabolic periodicities. | 138,454 | [
0.08031464,
-0.1210333,
-0.4982801,
0.0240738,
0.2523232,
-0.1577851,
0.01278458,
0.1407475,
0.3059257,
-0.3104866,
-0.05135252,
-0.1579764,
0.02990266,
0.3387891,
-0.4223366,
0.106729,
-0.663362,
-0.1124854,
0.09169152,
0.005770891,
0.2974499,
0.1071945,
-0.07636876,
0... |
Depressed responder and stimulator capacities of peripheral lymphocytes from patients with advanced breast cancer in the mixed lymphocyte culture. | Peripheral lymphoid cells of patients with carcinoma of the breast were examined for reactivity (responders) against allogeneic lymphoid cells in the one-way mixed lymphocyte culture. The latter cells were also tested for response against the patients' lymphocytes (stimulators). The responder and stimulator capacities of lymphoid cells of patients with advanced cancer were found to be significantly reduced compared to cells from healthy subjects. Such defects were not observed in disease-free women previously treated for primary carcinoma of the breast. The results support the view that there is an abnormality of the lymphocyte-monocyte pool of cells in patients with advanced cancer. | 138,477 | [
-0.07073674,
-0.2757258,
-0.2919143,
-0.2810343,
0.1935731,
-0.2821642,
-0.204244,
0.1216536,
-0.007714165,
0.3181458,
0.06137327,
0.1890536,
-0.08235278,
-0.01996954,
-0.4805726,
-0.1569221,
-0.4566189,
-0.04995717,
-0.2788143,
0.3245285,
0.5496774,
0.1184817,
0.1651078,... |
Occupational eczema from para-tertiary-butylphenol formaldehyde resins: a review of the sensitizing resins. | The first case of allergy due to para-tertiary-butylphenol formaldehyde resins (PTBP resins) were found in 1958 in the shoe-making industry. These resins are found in certain neoprene adhesives. PTBP resin hypersensitivity may be due either to the para-tertiary-butylphenol, to the resin itself or less frequently to the formaldehyde. These resins may cause occupational allergy (in the automobile industry and chiefly in the shoe-making industry) and non-occupational allergy (footwear and clothing hypersensitivity). Patients allergic to PTBP resins have been found to show a cross-reaction to other resins such as Alresen PA 103, Schenectady SP 126 and Schenectady SP 154. The authors give a reference list of sensitizing neoprene adhesives and PTBP resins so that manufacturers can limit the use of these products as far as possible. | 138,517 | [
-0.2374033,
0.1490294,
0.5387871,
-0.007722671,
0.299656,
-0.03429285,
-0.2763378,
0.1822836,
0.328895,
0.3152588,
0.2120544,
-0.2749422,
0.3348932,
-0.3103473,
-0.4059917,
-0.2962448,
-0.3771567,
0.3754753,
-0.1450294,
0.1051693,
-0.3659197,
0.243298,
-0.3056024,
-0.40... |
Contact dermatitis from tromantadine hydrochloride. | Of 240 patients treated with 1% tromantadine hydrochloride ointment, 20 showed local irritation, of which 12 were due to contact allergy as demonstrated by patch testing. | 138,520 | [
0.1156469,
-0.1923917,
-0.2500636,
-0.02800862,
0.06124817,
-0.143586,
-0.384569,
-0.3272067,
0.1113332,
-0.07448462,
0.1821642,
0.1517875,
0.2363766,
0.1219216,
0.1605151,
-0.2471296,
-0.3956085,
0.2021375,
-0.09828316,
-0.03989435,
0.05854441,
0.2151889,
0.1103023,
-0... |
Minocycline therapy in acne vulgaris. | A double-blind, random distribution study showed that a lower than recommended dose of minocycline--50 mg twice daily--was as effective as a dose of 250 mg twice daily of tetracycline for treatment of acne vulgaris in comparable patient groups, and that minocycline produced no vestibular side effects at the lower dosage. Like tetracycline, minocycline did not produce the phototoxicity associated with demeclocycline or the life-threatening colitis associated with clindamycin. Patients in this study did not develop a resistance either to minocycline or to tetracycline. Studies of the use of minocycline in patients who have developed tetracycline resistance and long-range studies of patients on the new lower dose of minocycline are now underway. | 138,560 | [
-0.005995986,
-0.2695186,
0.03070061,
-0.06283793,
-0.008366853,
-0.2374252,
-0.200184,
-0.07967265,
0.03721204,
-0.0792171,
-0.09807914,
0.03649829,
0.1140084,
0.1434266,
0.1898007,
-0.1516281,
-0.5015645,
0.427279,
-0.2397222,
0.02283037,
0.0226172,
0.06938121,
0.150727... |
Replication of chromosomal DNA in diploid Drosophila melanogaster cells cultured in vitro. | Replication rate and replicon sizes in chromosomal DNA of in vitro cultured diploid D. melanogaster cells were determined using autoradiography of 3H-thymidine labeled DNA. Synthesis of DNA in euchromatic and heterochromatic regions of Drosophila diploid cells occurs at different periods of the S phase which last 10 h. During the first 4 h the synthesis is observed only in euchromatic regions. The heterochromatic synthesis starts shortly before the synthesis in euchromatic regions is completed and lasts for 6 h until the end of the S phase. The cells were synchronized by 5-fluorodeoxyuridine which blocked the diploid cell DNA synthesis. Synthesis was found to start simultaneously in most euchromatic replicons. In the majority of the replicons the synthesis started at a single point and proceeded bidirectionally. The average rate of DNA synthesis per fork was 12.5 mum/h (38kb). The mean distance between the middle points of adjacent labeled regions was 70 mgm (210 kb). The size of most replicons ranged from 40 to 120 mum. - These estimates do not apply to the heterochromatic portions of the D. melanogaster genome since the measurements have been carried out on DNA preparations obtained during the first 2 h of the S phase. - On the average, a replicon can consist of 7 chromomeres since the size of a replicon in diploid cell chromosomal DNA and DNA length of a polytene chromomere average 210 and 30 kb, respectively. | 138,582 | [
0.0302528,
-0.1942965,
-0.1885258,
0.1047407,
0.131148,
0.1476627,
0.007469743,
0.1278193,
0.1394016,
0.01580862,
0.1701951,
-0.01048387,
0.05221048,
0.1752066,
-0.7270573,
-0.06178677,
-0.216903,
0.2173827,
0.02645325,
0.1644801,
0.4385355,
0.08204158,
-0.1449392,
-0.3... |
[Effect of parathyroid hormone on electrical and contractile processes in myocardial cells]. | In normal ionic milieau, the parathyroid hormone (PTH) generated a biphasic effect: the initial increase in amplitude and duration of A at the plateau level, and the following decrease in amplitude and duration of AP of the myocardial cells at every level of repolarization. The contractile activity steadily decreased under the PTH effect. The negative inotropic effect of the PTH in its quantitative and temporal aspects was diminished by a preliminary administration of calcitonin. The PTH effect is supposed to be based on an increase in the calcium flow through slow CaNa channels and on Ca binding in intracellular sites of storage. | 138,601 | [
-0.0459223,
-0.09292502,
-0.2935242,
0.003812245,
-0.06991739,
-0.2425041,
-0.5239559,
0.2079118,
0.1267992,
0.01081698,
-0.1348272,
0.06594905,
0.02344833,
-0.07175274,
-0.4612487,
-0.1062899,
-0.4164941,
-0.07532046,
-0.03190164,
0.08933248,
0.7453166,
0.02591263,
0.034... |
[Lung function scintigraphy in the diagnosis of regional disturbances of ventilation (author's transl)]. | Lung function scintigraphy is the method of choice for the diagnosis of regional ventilation disturbances. The aim of this study was to determine which of various parameters is the most reliable. Sensitivity of the 3-minute retension test is 96%, of the single breath 45%, equilibration 32% and of the ventilation index, only 28%. Sensitivity of the various parameters for demonstrating multi-focal ventilation abnormalities is 100% for the 3 minute retention test, 32% for single breath, 19% for equilibration and 21% for the ventilation index. The underlying basis for these findings is discussed and it is concluded that it is possible to simplify the technique when dealing with regional ventilation abnormality in obstructive airway disease, but not when dealing with other restrictive changes. | 138,634 | [
0.1172819,
-0.2345306,
0.001739747,
-0.09514461,
0.09354586,
-0.3106906,
-0.1226811,
-0.216232,
-0.1447283,
0.1147318,
0.1029077,
-0.1061808,
-0.2755333,
0.1204875,
-0.3173448,
-0.3876656,
-0.5198458,
0.3953717,
0.05345324,
0.1034626,
-0.02270823,
0.03243881,
0.104706,
... |
[The radiological diagnosis of non-calculous biliary duct obstruction (author's transl)]. | A diagnosis of obstructive biliary duct disease was achieved in 80% of 63 patients using endoscopic retrograde cholangiopancreatography (ERCP) and percutaneous trans-jugular cholangiography (PTJC) alone or in combination, and taking account of the clinical features. In obstructions of themajor intrahepatic ducts and of the hepatic ducts (type I) a smooth occlusion indicated a carcinoma. Narrow forms of stenoses could not be differentiated, but with a history of previous operation, fibrous strictures were most likely. Obstructions at the point of confluence (type II) showed smooth narrowing if due to fibrous strictures, whereas carcinomas produced an irregular termination. Obstructions of the common bile ducts (type III) resembled those of type II. A smooth termination within the pancreas indicates a pancreatic carcinoma. Inflammatory disease in the head of the pancreas usually produces a tubular stenosis, while cysts of the pancreas result in smooth impressions and displacement. | 138,635 | [
0.1544372,
0.06564297,
0.2300265,
-0.005551894,
-0.02720636,
-0.1415007,
-0.1335633,
0.1680903,
0.1813403,
0.3042659,
0.08269943,
-0.479646,
-0.2188506,
-0.1176175,
-0.5490372,
-0.531765,
-0.2068879,
0.2854397,
0.2928588,
0.06457698,
0.1775363,
-0.1933166,
-0.007992732,
... |
[Multiple occlusions and stenoses of the abdominal aortic branches with development of Riolan's collateral and hypertension (author's transl)]. | One case of multiple occlusions and stenoses of the abdominal aortic archs with development of Riolan's collateral in a young patient with hypertension is described. In the literature some cases are pointet out, which clinically resemble our patient and where the aetiology is not clarified. -- In the discussion about the aetiology of this vascular anomaly a developmental anomaly and mechanical compression by the crura diaphragmatica are named. We think, that an unspecific arteriitis similar to the in Japan well known Tacaysau-Aortitis must also be discussed. This unspecific arteriitis of the abdominal aorta and its branches would explain the occlusion and stenosis of several arteries with development of Riolan's collateral and manifestation of hypertension. | 138,638 | [
-0.02307438,
0.4358033,
0.08961709,
0.1231385,
0.4242325,
-0.102146,
-0.2675416,
-0.09465015,
0.02971132,
0.1665155,
-0.05952307,
-0.1730587,
0.1613493,
-0.3458669,
-0.4957774,
0.1543769,
-0.1903743,
0.07133994,
0.2269116,
-0.4328153,
0.2621079,
-0.06873155,
-0.2825232,
... |
[Late meningeal changes due to water-soluble myelographic contrast medium (author's transl)]. | Late meningeal changes due to various contrast media of the water-soluble type used for radiculography were analysed and the causes for the changes are discussed. The osmotic properties of the substance are an important aspect of the contrast medium. Hints for avoiding late complications are given. | 138,641 | [
-0.2457139,
-0.003748639,
-0.07589646,
-0.06927944,
0.02650366,
-0.4198877,
-0.4067067,
-0.3637607,
0.001435188,
0.1920799,
0.05508493,
0.2095188,
0.1663289,
-0.12329,
-0.4451667,
-0.1076111,
-0.05183581,
0.2627982,
-0.1755898,
0.1420317,
0.3780672,
0.03623649,
0.07801753... |
[Radiological observations of the chest in progressive scleroderma (author's transl)]. | All available chest radiographs of 50 patients with progressive scleroderma have been freshly evaluated. In 28 cases (56%), pulmonary involvement was diagnosed; its type and severity was classified according to the specimen films of the ILO-U/C 1971 classification for pneumoconiosis. Serial observations in 27 patients spanning a period of one to 21 years have shown that the course of the disease in the lung is unpredictable. Rapid progress of fibrosis is, however, uncommon. Two radiological appearances in the thorax, which are not specific, but are found predominantly in scleroderma are: 1 an air oesophagram on the lateral thorax film (8 cases) and erosions of the upper rib margins (5 cases). The importance of radiological examination lies in the fact that it confirms the clinical diagnosis and that it may indicate the cause of an otherwise undiagnosed pulmonary fibrosis. | 138,652 | [
0.05523189,
-0.1020872,
0.2991957,
-0.2325354,
0.08756024,
-0.05548755,
-0.1886966,
-0.2880719,
0.1940068,
0.1174168,
0.1062887,
-0.1952965,
0.1427639,
-0.150606,
-0.4554947,
-0.09222989,
0.02533976,
0.1115838,
0.3321499,
-0.1189248,
0.4254228,
0.3219671,
-0.05693149,
0... |
Reaction of cardiac myosin with a purine disulfide analog of adenosine triphosphate. I. Kinetics of inactivation and binding of adenylyl imidodiphosphate. | Bovine cardiac myosin ATPase activity was rapidly inactivated by the purine disulfide analog of ATP,6,6'-dithiobis(inosinyl imidodiphosphate). Kinetic investigations showed that this analog acted as a site-specific reagent at 0 degrees with a Ki of 130 muM and a half-life of 8.2 min at saturating inhibitor concentrations. Concentrations (50 to 500 muM) of ATP, adenyl-5'-yl imidodiphosphate (AMP-PNP), or ADP that saturated the active site caused an enhancement in the rate of inactivation, indicating the purine disulfide analog was not reacting at the active site. Under these conditions saturation kinetic data were still observed with Ki values remaining unchanged (120 muM) but with the half-life of inactivation decreasing to 6.0 min (ATP) and 4.6 min (AMP-PNP) at saturating inhibitor concentrations. At concentrations greater than 0.5 mM ATP, AMP-PNP, or ADP there was a decrease in the rate of inactivation, implying protection by these nucleotides. However, saturation kinetics of inactivation could no longer be demonstrated, implying a change in the mechanism of inactivation. A comparison of the inactivation of the Mg2+, Ca2+, and EDTA-ATPase activities of cardiac myosin after modification by the purine disulfide analog showed that the Mg2+- and Ca2+ATPase activities plateaued at approximately 60% and 40%, respectively, while the EDTA-ATPase activity continued to decrease to below 10%. This evidence supports the suggestion that the purine disulfide analog was not reacting at the active site. Equilibrium dialysis experiments were used to measure the binding of [8-3H]AMP-PNP to native cardiac myosin, the thiopurine nucleotide-modified myosin, and the derivative formed by displacing the thiopurine nucleotide by cyanide (thiocyanato-myosin). Native myosin bound a total of 2.1 mol of AMP-PNP with a binding constant of 6.0 X 10(6) M-1. There was a 15 to 40% decrease in the number of AMP-PNP binding sites in the enzyme derivatives, but the active sites appeared not to be blocked since the association constants remained essentially unchanged (KA=3.9 X 10(6) M-1 for thiopurine nucleotide-myosin and 12.0 X 10(6) M-1 for thiocyanato-myosin). The kinetic studies and the binding experiments indicate that the purine disulfide analog reacts at a specific site other than the active site but do not offer support to earlier suggestions from skeletal myosin studies that this site is a possible ATP control site. | 138,683 | [
-0.2933848,
-0.1169134,
-0.4389741,
-0.06144037,
0.1856423,
-0.0116904,
-0.004381965,
0.3841645,
0.03302866,
-0.08131391,
0.08930337,
0.1836637,
0.1679198,
0.3489719,
-1.03852,
0.1086895,
-0.4576291,
-0.06361811,
-0.3202137,
0.5149903,
0.3739552,
0.1416065,
-0.1794234,
... |
Action of FdUrD and dCyd on the incorporation of BrdUrd in chinese hamster somatic cell DNA and the isolation of auxotrophic mutants. | Chinese hamster somatic cells grown in the presence of bromodeoxyuridine, deoxycytidine and fluorodeoxyuridine incorporate more bromodeoxyuridine in the DNA than cells grown in the presence of bromodeoxyuridine alone. Thus they become more sensitive to light irradiation. Our data suggest that 0.05 mM--0.2 mM bromodeoxyuridine, 0.05 mM deoxyctidine and 10 mmug/ml fluorodeoxyuridine is one of the best possible combinations for the selection of Chinese hamster somatic cells mutants. Auxotrophs for proline, inositol or both were thus isolated at high frequency. | 138,686 | [
-0.0949636,
-0.4709625,
-0.09560453,
0.05957916,
0.3186878,
-0.3595355,
-0.1492503,
-0.01476756,
0.10719,
-0.05332031,
0.01856464,
0.1053253,
-0.1135186,
0.1266722,
-0.786,
-0.1397485,
-0.4906321,
0.004835747,
-0.249089,
0.5847724,
0.4011456,
0.432822,
0.0700795,
-0.127... |
Allergic contact dermatitis from ultraviolet cured inks. Allergic contact sensitization to acrylates. | Eight men employed in the manufacture of ultraviolet cured inks developed allergic contact dermatitis predominantly on the exposed areas. Patch testing revealed sensitization to trimethylol propane triacrylate in seven employees, to 1,6-hexanediol diacrylate in six employees, to pentaerythritol triacrylatylate in four employees and to epoxy acrylate oligomers in three employees. Either cross-sensitization or concomitant sensitization may have accounted for the multiple reactions in several employees. One sensitized employee was patch tested with four different commercially available epoxy acrylate oligomers and reacted only to two, suggesting that variations possibly in chain length between these oligomers are important variables in the allergic reactions. The polyfunctional acrylic monomers and certain epoxy acrylate oligomers should be handled carefully to avoid the development of allergic contact dermatitis. | 138,725 | [
-0.1272545,
0.006553433,
0.1753012,
-0.03486786,
0.1332356,
-0.1830156,
-0.0447143,
0.2844883,
0.2604004,
0.3587443,
0.1536647,
-0.2663511,
0.1020028,
-0.3000543,
-0.4589514,
0.1624424,
-0.2221192,
0.5989994,
-0.2316401,
0.1584378,
0.05939062,
0.1166075,
-0.08680819,
0.... |
[Diagnosis of cardiomyopathy]. | The problems of diagnosis of cardiomyopathies are discussed. Among 13 patients who died in the clinic, a correct diagnosis was made in 10. The observations permit to believe that cardiomyopathy is seen more often in males aged 41 to 55 years. The course of the disease is chronic recurrent, its onset being different in individual cases: rhythm disorders, cardiac pains, recurrent pulmonary infarctions, cerebral circulation disorders. The outcome of the disease consisted, as a rule, in progressive total cardiac insufficiency refractory to therapy. In all the cases cardiomegaly was observed, not so much at the expense of hypertrophy, but rather of dilatation of the cardiac chambers. The anatomic signs included, apart from the cardiac dilatation, moderate hypertrophy with a relative bi- and tricuspid valve insufficiency, also mural thrombi in the heart chambers. | 138,763 | [
0.029162,
0.214474,
-0.1496008,
-0.2299458,
0.02559752,
-0.4290026,
-0.02232612,
-0.07045432,
0.006410395,
-0.292928,
0.08915821,
0.2773942,
-0.07744163,
-0.09759422,
-0.2811456,
-0.1165654,
-0.1920568,
0.302358,
-0.02341169,
0.2641011,
0.3698007,
0.03153405,
-0.06255246,... |
Increased mobility with an articulated lens in gynecologic endoscopic photography. | A new device in endoscopic photography, called an articulated lens, allows a video or movie camera to be separated from the laparoscopic endoscope, thereby removing the camera from the operating field and allowing the laparoscopist improved maneuverability during photographed procedures. By forming a segmented optical link between the telescope and the television or movie camera, the lens allows the two units to be separated by as much as 3 ft and permits the endoscope to move easily in all planes, horizontally, vertically and diagonally. This improved video and cinematographic technique enchances the teaching of surgical procedures and provides the means for keeping permanent records of such procedures. | 138,782 | [
-0.1271796,
0.1504556,
-0.2001054,
-0.1519637,
-0.06614404,
-0.4110233,
0.03377753,
-0.2705128,
0.219058,
0.002467819,
0.1071904,
-0.09302121,
0.01457378,
-0.2299787,
-0.1938496,
0.1367874,
-0.7354839,
-0.03833334,
0.08666096,
-0.3258467,
0.2480781,
-0.04577764,
-0.412437... |
[HBs-antigen induced extrahepatic immune-complex disease (author's transl)]. | A 62-year old patient was hospitalized with fever, polyarthritis and exanthema. All bacteriological, virological and biochemical findings were normal. The immunological analysis showed initially the HBs-antigen in the serum. No irregular antibody was found. Chemotherapy was failing, but application of corticosteroids was followed by a dramatic improvement. Nine weeks after the onset of the disease the patient was healthy again. Antibodies against HBs-antigen were found 3 months later. The data suggest an immune-complex disease like serum sickness based on an infection with hepatitis-B-virus without involvement of the liver. | 138,790 | [
0.02220433,
0.03403628,
-0.02772677,
0.08620878,
0.03470832,
-0.3745716,
-0.1238633,
-0.05111483,
-0.06860141,
-0.06797379,
0.1307654,
0.2004988,
0.1556284,
0.2459592,
-0.2743653,
-0.0881586,
-0.1934735,
0.03660113,
0.1987341,
0.4728136,
0.2694196,
-0.1180139,
-0.1094746,... |
[Susceptibility of laboratory animals to Trichinella from synanthropic and natural foci of the northern Caucasus]. | Laboratory animals were infected with larvae of four species of Trichinella. The tests have whown that T. pseudospiralis is highly infectious for golden hamsters and guinea pigs. Rats are relatively resistant to T. pseudospiralis but highly susceptible to T. spiralis. T. nelsoni turned out to be little infectious to rodents which were used in the experiment. T. nativa is little infectious for rats but more infectious for golden hamsters and guinea pigs as compared to T. nelsoni. | 138,835 | [
0.2922133,
-0.3367293,
-0.092791,
0.02848989,
0.1053547,
-0.3569934,
-0.4597562,
-0.05784367,
0.1584238,
-0.2184151,
0.10949,
-0.01107472,
0.1888871,
-0.1905154,
-0.3854729,
-0.5026397,
-0.4706202,
0.113164,
-0.004357195,
0.1728812,
0.09168039,
0.2200053,
-0.1286548,
0.... |
[Therapeutic management of neoplastic occlusions of the colon]. | The authors present their experience in 39 cases of occlusions occuring in a number of 142 neoplasies of the colon hospitalized between 1965 and 1973. The major attention was given to the treatment of the occluding complication. In cases where the occlusions are due to neoplasies of the right colon, right hemicolectomy should be carriet out in preference, while in cases with neoplastic occlusions of the left colon the attitude will be differentiated. The authors are not partisans of the emergency left hemicolectomy. | 138,869 | [
0.1196061,
0.3842058,
0.06998348,
-0.06951594,
0.02778758,
-0.2991537,
-0.1955894,
-0.3905931,
0.003109236,
0.05268267,
0.0272514,
0.2609731,
-0.0004965142,
-0.2964704,
-0.1190579,
-0.2219221,
-0.4720499,
-0.01178318,
0.1602738,
-0.02865644,
0.3566323,
0.2484969,
0.048720... |
[Current aspects of infectious mononucleosis]. | The authors discuss present aspects in infectious mononucleosis, with reference to the cases admitted to the Clinic of communicable Diseases of Cluj-Napoca, i.e. 115 cases during the 1954--1973 period (3 to 13 cases a year). In most cases the clinical symptomatology was mild and the laboratory tests typical not giving rise to any diagnostic difficulties. However, 28 cases (25%) presented jaundice, with positive hepatic tests, so that a differential diagnosis was established with viral hepatitis. In 9 cases nervous manifestations were reported: 4 encephalitis, 1 neuritis of the hypoglossal nerve, 2 facial paralysis, 2 benign serous meningitis cases, all of which fully recovered. The Paul Bunnel-Hăngănuţiu test was positive in titers of 1 : 40 -- 1 : 1280, in all the cases. Corticotherapy was applied with good results in 24 cases. All the cases ran a favourable course. On admission, 45% of the patients came with a diagnosis of infectious mononucleosis; the others were admitted with a diagnosis of : diphteric angina, viral hepatitis, influenza, German measles, mups, typhoid fever, prolonged febrile syndrome. | 138,923 | [
-0.1647623,
-0.3916325,
0.1401988,
-0.413005,
0.2255591,
-0.280657,
-0.5920134,
-0.05576033,
-0.04507575,
0.02254807,
0.2358243,
-0.1004342,
0.1703575,
-0.2607924,
-0.4287995,
0.1241742,
0.02746255,
0.2579952,
-0.2389486,
0.06317966,
0.4792373,
-0.4426678,
0.06729788,
-... |
[Distribution of capsular serotypes in Klebsieela strains of enteral origin]. | The significant proportion of microorganisms belonging to the Klebsiella genus among the causative agents isolated and subdifferentiation of the genus for epidemiological survey of infantile Klebsiella infections are dealt with in the present paper. The 28 Klebsiella strains isolated from cases of enteritis confirms the enteral tropism of these enterobacteria, especially among the infantile population. A proportion of 13.3% strains were identified in pure cultures obtained from feces and were considered as certainly enteropathogenic. The biochemical behaviour of the strains studied pointed to the ubiquity of the aerogenes biotype (76.9%) followed by the pneumoniae biotype (12.4%) and indol-positive Klebsiella strains (10.7%). Of the total 308 Klebsiella strains typed serologically with 80 OK capsular sera, 284 (92.2%) were listed in 63 serotypes, 13 of which (K7, K35, K47, K9, 530, K16, K1, K11, K18, K20, K37, K80, K i.e. 47.5%) were frequently identified in acute infantile cases of enterocolitis syndrome. Most of these serotypes are also considered endemo-epidemic by authors. Worthy of note among the Klebsiella types isolated in pure cultures from cases of infantile enteritis, was the prevalence of the aerogenes biotype, with a higher incidence of K1, K2, K16, K26, K44 serotypes, and of the indol-positive strains, with serotypes K13, K16, K65, K80. | 138,927 | [
-0.2616313,
-0.3136847,
0.08952928,
0.09104498,
-0.2528856,
0.02814854,
-0.1631892,
0.08665828,
0.03497281,
0.03755479,
-0.01729076,
-0.2614873,
-0.06598868,
0.3386295,
-0.3768367,
-0.2521598,
-0.5980916,
0.287854,
0.4061332,
-0.1039948,
0.3493997,
0.3175901,
-0.1389319,
... |
Studies on activator formation in human plasma with streptokinase. III. Investigation of activator kinetics in undiluted plasma in terms of urokinase equivalents. | A new method is presented for estimating the activator (plasminogen-streptokinase complex) concentration in native plasma of patients undergoing streptokinase infusion. The principle of the method is based on clot lysis time as recorded by the thromboelastograph. The test clot constituents were bovine fibrinogen, bovine plasminogen, EDTA, human plasma (with unknown activator concentrations), and thrombin. In order to obtain a standardization line, urokinase dissolved in NaCl solution was substituted for patients' plasma. Thus, each lysis time could easily be converted into urokinase equivalent (CTA-u/ml). Streptokinase and plasminogen molecules in undiluted patients' plasma were found to exist both in an activator-bound (equimolar plasminogen-streptokinase complex) and in a freely circulating form. This result is in agreement with earlier findings where the activator complex was demonstrated to be a widely dissociated complex in highly diluted plasma of patients, thus displaying an ample proportion of free streptokinase and plasminogen and molecules. Streptokinase treatment using dosage schemes of 100,000 u SK/h, and 200,000 u/h were monitored by quantitative activator, streptokinase, and plasminogen measurements. An average activator concentration of 50-100 CTA-u/ml and a SK-concentration of 7-16 u/ml were recorded during streptokinase infusion. Plasminogen values averaged 0.25%, independent of the amount of streptokinase infused. Each drop in streptokinase was accompanied by a drop in activator during the infusion, and each rise in streptokinase by a rise in activator. There was a strong correlation between streptokinase and activator concentrations in that, on the average, 1 u streptokinase equalled 8.4 CTA-u/ml activator (correlation coefficient r = 0.9) It is concluded that the activator concentration in the plasma of patients undergoing fibrinolytic treatment can easily be adjusted by regulating the hourly streptokinase influx. | 138,962 | [
-0.001882057,
0.1311514,
-0.0144977,
-0.1489876,
0.3514001,
-0.2804363,
0.01342245,
0.2692805,
0.1085046,
0.05268373,
0.05226606,
0.3138391,
-0.04980803,
-0.2150497,
-0.03012297,
-0.002618646,
-0.3515195,
-0.01276561,
-0.04398625,
0.3210745,
0.4758525,
0.1388279,
0.011667... |
Intra- and extrarenal vascular changes in the acute renal failure of the rat caused by mercury chloride. | Histologic evidence of intrarenal vasomotor changes were observed in the rat in the course of acute renal failure caused by the injection of HgCl2. Male Wistar rats injected s.c. with 2.5 or 4.7 mg HgCl2 per kg b. wt. developed fibrinoid damage in the media segments of preglomerular renal vessels, mostly in the arcuate and interlobular arteries. The lesions were patchy and irregularly scattered throughout the kidneys. 24 h post-injection the lesions were very rare and of only mild degree, whereas they were fully developed and regularly seen 48 h post-injection. A high percentage of similar changes was found in certain extrarenal vascular areas especially in the mesentery and pancreas. The damaged vascular segments were usually dilated. The results of various thichrome stains and histochemical reactions suggested edema of vascular smooth muscle cells and imbibition of the media by blood plasma substances, sometimes reaching the degree of fibrinoid necrosis. These findings were confirmed by electron microscopy. The imbibition of the smooth muscle cells by blood plasma material was clearly evidenced by the demonstration of intracellular fibrin precipitations. In connection with the degeneration of smooth muscle cells, accumulations of crystal-like fibrin formations could often be shown. Subendothelial fibrin formations were not observed. 96 h after the 2.5 mg injection the changes were already regressing, but edema of the vascular wall and signs of disturbed vasotonia persisted for several days. The maximum of the vascular changes usually coincided with the maximum of azotemia and the formation of debris cylinders in the renal tubules. However, no clear relationship was recognizable in individual cases between vascular damage, extent of tubular necrosis and renal function. The pathogenesis of the vascular changes is obscure, but neurogenic factors, increased release of catecholamines and/or vasoactive agents of renal origin in connection with other factors might play a decisive role. Arterial hypertension was absent. It is assumed that the structural damage of the vascular media is mainly brought about by prolonged or recurring vasospasms, or by alternating spasm and vasodilatation with local ischemia and increased tension of the vascular wall in the dilated segments. The altered function and structure of the vascular wall might, to a certain extent, contribute to renal insufficiency. | 139,013 | [
-0.24796,
-0.05930853,
-0.07791346,
-0.1011729,
0.3723288,
-0.07781775,
0.1080296,
0.03251826,
-0.07488389,
0.3160962,
0.07648605,
-0.3332035,
-0.2216166,
0.08834177,
-0.3963782,
0.3029346,
-0.1130193,
0.2925484,
0.01761934,
0.1987443,
0.2777019,
0.4836797,
0.07121292,
... |
[Pulmonal silicone-embolism. Light microscopic, electron microscopic and enzymehistochemical investigations in the rabbit lung (author's transl)]. | Artificial silicone-embolism in rabbits was produced by injection of Baysilon-antifoam into the ear vein. Electron microscopic and histochemical observations of the lung were made, concerning the toxicity and elimination of the embolic material. At 13 months after injection the embolic silicone was not significantly reduced in amount. The rabbit lung is unable to eliminate chemically inert silicone by enzymatic degestion. We noted serious defects of endothelial cells and pneumocytes, destruction of capillaries and local atelectases. These defects are preponderantly mechanical in origin, but may be caused by lack of substrate or energy in cells deprived of their blood supply. Atelectases are interpreted as a result of break-down in surfactant-synthesis. | 139,024 | [
0.1328771,
0.137754,
-0.3452529,
0.2052015,
0.03301436,
-0.1423023,
-0.2202166,
-0.2853734,
-0.001165549,
0.1156937,
-0.1758351,
0.1631293,
-0.2057198,
-0.407519,
0.06055909,
0.2181825,
-0.5088677,
0.1708746,
0.4061753,
0.1268029,
0.5507604,
0.2505921,
-0.1552721,
-0.05... |
[Studies on the thymidine-triphosphate synthesis in malignant tumors. II. Effect of hyperthermia, Vitamin K and Cytotoxic agents (author's transl)]. | Measurements of the deoxyribonucleoside triphosphate (dNTP) contents, the [14C] thymidine and deoxyuridine incorporation and the "key enzymes" of the thymidine triphosphate (dTTP) synthesis, thymidine kinase and ribonucleotide reductase, in diploid Ehrlich-ascites carcinoma, under the application of hyperthermia, vitamin K and cytocidal agents show: The effect of hyperthermia and menadion (the basic substance of the K vitamins) on the above parameters of dNTP synthesis can explain the labile effects of hyperthemia and vitamin K therapy on cancer growth. Alterations of the dNTP concentrations and demonstrable or absent inhibition of the ribonucleotide reduction with application of fluoruracil, amethopterine, cytosine arabinoside, hydroxyurea, trisethylen iminobenzochinone and daunomycin confirm and supplement our knowledge of the cytostatic action mechanism of these substances. They show moreover by the example of fluoruracil and amethopterine medication that the dTTP concentration estimation after in-vitro incubation of tumour cells with the addition of FU or methotrexat is a better measurement of the therapeutic in-vivo responsiveness of malignant tumours than the previously performed test methods. | 139,037 | [
-0.2483544,
-0.311745,
-0.1486225,
-0.2484469,
0.464116,
-0.1568204,
0.1746261,
-0.18528,
-0.0410826,
-0.09427152,
0.1794275,
0.1721424,
0.1720664,
0.3219414,
-0.5762616,
0.05162696,
-0.380615,
0.1684862,
-0.3345114,
0.3758766,
0.5544574,
-0.3942191,
-0.1560395,
0.14977... |
[Value of puncture-lavage in abdominal closed trauma. Comparison with the laparoscopy and symptomatology (author's transl)]. | In a series of 120 closed abdominal trauma the authors compare the reliability of the abdominal symptomatology (60%), of puncture-lavage with an Intracath (92.5%) or with a Diacath (98%) and of laparoscopy (100%). Laparoscopy and puncture-lavage with a Diacath have the same advantages: rapid execution, safeness, reliability. However the preference of the authors goes to the catheter and peritoneal dialysis because of its simplicity and feasability by the surgeon himself. This technique discloses visceral lesions: if positive it allows an earlier diagnosis and operation; if negative it avoids unnecessary laparotomies especially in cases of multiple trauma. | 139,070 | [
-0.2329578,
-0.0500636,
-0.1193667,
0.05526133,
-0.1374459,
-0.2214061,
-0.6295812,
-0.2171704,
-0.1221651,
-0.1355646,
0.2756385,
-0.0994639,
-0.08598275,
-0.2367066,
-0.1734962,
-0.5801117,
-0.6489601,
-0.1195169,
0.02840818,
-0.01108098,
0.09268452,
-0.2096177,
-0.1307... |
Effect of a selective 5-HT uptake inhibitor--Lu 10-171--on rat brain 5-HT turnover. | Changes in endogenous concentrations of 5-HT and 5-HIAA as well as the turnover rate of 5-HT was studied in rat brain after treatment with a new potent and selective inhibitor of the neuronal 5-HT reuptake mechanism, Lu 10-171(1-(3-dimethylamino)propyl)-1-(p-fluorophenyl)-5-phthalancarbonitrile). After a single dose of Lu 10-171 the concentration of 5-HIAA was reduced from 1 to 24 hours after treatment, whereas that of 5-HT was practically unchanged, indicating decreased turnover of 5-HT in the brain. This was confirmed using three different methods for measuring 5-HT turnover. Thus the rate of 5-HIAA accumulation in the brain after probenecid was reduced after Lu 10-171. Likewise treatment with Lu 10-171 led to a decreased fall in 5-HT and an increased fall in 5-HIAA after inhibition of 5-HT synthesis with parachlorophenylalanine (PCPA). Unexpectedly Lu 10-171 did not change either the accumulation of 5-HT or the decrease in 5-HIAA after inhibition of MAO with pargyline. By and large the results are consistent with the proposed negative feed-back regulation of 5-HT neuronal firing rate due to the abundance of 5-HT at postsynaptic receptors after inhibition of the reuptake mechanism. | 139,078 | [
-0.1927109,
-0.2601163,
0.04588173,
-0.4430541,
0.1311868,
0.03317133,
-0.6340462,
0.003425267,
-0.03703686,
0.09296192,
-0.08565046,
0.3628072,
0.549037,
0.04714117,
-0.4880385,
-0.07080822,
-0.2570764,
0.2283233,
-0.1263712,
0.3111647,
0.1865231,
0.4617208,
0.09852275,
... |
[Demonstration of antigens of bone and articular connective tissue in the blood of rats with adjuvant arthritis]. | The results reported in this paper clearly indicate the release of osteal antigens of articular connective tissue in response to inflammatory alterations and injuries to the region immediately adjacent to an articulation. Further studies will have to be performed in an effort to find out whether these antigens are of pathological importance and if there is a relationship between the liberation of antigens and the intensity of inflammatory alterations or if the different and often difficult-to-interpret results of the determination of antibodies in the sera of patients with rheumatoid arthritis can be more easily explained by a simultaneous antigen analysis of the corresponding sera. | 139,092 | [
0.08416381,
-0.0564237,
0.203001,
0.03414068,
-0.2463575,
-0.3951458,
-0.3658434,
0.3274572,
0.01621045,
0.1917046,
-0.04882907,
-0.07624994,
0.3765462,
-0.242188,
-0.5027254,
-0.06792504,
-0.1344298,
-0.1122756,
0.02782763,
0.5412807,
0.3038057,
-0.1845964,
-0.1269066,
... |
Tightly bound nucleotides of the energy-transducing ATPase, and their role in oxidative phosphorylation. II. The beef heart mitochondrial system. | 1. Beef heart mitochondrial ATPase, in both the membrane-bound and isolated form, contains tightly bound ATP and ADP. Each mol of ATPase contains about 2.2 mol ATP and 1.3 mol ADP. 2. In the absence of ATPase activity, these nucleotides exchange only slowly with nucleotides in solution. The exchange rate is increased during coupled ATPase activity, but not when the ATPase is uncoupled. 3. Oligomycin and dicyclohexylcarbodiimide inhibit exchange of the bound nucleotides, as does the ATPase inhibitor protein, although in each case some residual exchange occurs. Aurovertin, although inhibiting phosphorylation, does not inhibit the exchange. This is discussed in terms of the reversibility of these inhibitors. 4. The stimulation of exchange seen during coupled ATPase activity requires energisation of the ATPase molecule. Using the exchange reaction as a probe of energisation, it is deduced that energy can be transferred between different ATPase molecules. 5. It is proposed that coupled ATPase activity and phosphorylation in submitochondrial particles involve the tight nucleotide binding sites and the (weak) ATPase site, while uncoupled ATPase activity involves only the weak site. | 139,163 | [
-0.3611434,
0.2627966,
-0.1686814,
0.05639371,
0.2325231,
-0.1698322,
0.1332749,
0.347434,
-0.03407651,
-0.09726444,
0.05117653,
0.273419,
0.09241407,
0.03432228,
-0.4373562,
-0.08504674,
-1.093284,
0.09131168,
-0.1306167,
-0.2220511,
0.4149189,
0.1133808,
-0.4082197,
0... |
[Role of the kidneys in the pathogenesis of lithium poisoning]. | Lithium intoxication was induced in rats by intraperitoneal administration of lithium chloride in a daily dose of 200 mg/kg (0.22 LD50) for 6 days. Polyuria connected with pathological changes in the epithelium of the convoluted tubules and depression of the antidiuretic hormone--acid mucopolysaccharides system in the area of the straight kidney tubules was observed on the 6th day of the experiments. Oligouria and death of some of the animals on the 7th experimental day was caused by severe lesions the kidney structure. Further observation (30 days) demonstrated that, along with the regeneration processes, there developed a marked sclerosing ofthe kidney tissue. A conclusion was drawn that severe lithium intoxication was associated with the development of acute renal insufficiency. Functional reserves of the kidneys after the cessation of lithium chloride administration remained lowered for a long period. | 139,180 | [
-0.04158082,
-0.3700528,
0.01402076,
0.1971378,
0.276057,
-0.3845222,
-0.2319107,
0.0185719,
-0.4488449,
0.0322945,
0.07764352,
0.1748821,
0.06438642,
0.2353247,
-0.4803565,
0.068573,
0.1047061,
0.175797,
0.2405285,
0.3406166,
0.4594358,
0.3353144,
-0.2100791,
-0.198162... |
[Role of renal function and calcemia in demonstration of kidneys in 99mTc-diphosphonate skeletal scintigrams (author's transl)]. | Normally, in 99mTc-diphosphonate-skeletal scintigrams the kidneys are delineated with the same intensity as the lumbar spine; This is not the case in patients with reduced renal function. In a series of 20 patients with varying degrees of renal insufficiency, a continuous decrease in the renal intensity with increasing serum creatinine was seen: clearly recognizable decreased renal intensity in patients with serum creatinine over 2 mg%, and no visualization of the kidneys in patients with serum creatinine over 8 mg%. This effect is intensified through a simultaneously existing hypercalcemia has the opposite effect, i.e., the intensity decrease is partially prevented. In patients with normal renal function a hypercalcemia even leads to an increased renal intensity. As a further cause for excessive renal intensity, an increased parenchymal transit time was found in the radionephrography in 5 out of 11 patients with normal serum creatinine in whom the increased renal intensity in the skeletal scintigram was a chance finding. | 139,343 | [
0.003496508,
-0.1205895,
0.01306451,
-0.03841269,
0.04615973,
-0.2818242,
-0.2486647,
-0.1541189,
-0.3154784,
0.1665473,
0.09051753,
0.1441354,
0.1115937,
-0.1335697,
-0.5895624,
-0.1800634,
-0.2756339,
0.1638725,
0.04180021,
0.2582654,
0.3979005,
0.05876917,
-0.03122033,... |
The application of economic analysis to evaluation of alcoholism rehabilitation programs. | We have presented an algorithm designed to facilitate the application of economic analysis to the evaluation of individual alcoholism rehabilitation programs. In doing so we have attempted to delineate the limitations of such analyses as well as the type and value of information they provide. After a brief review of the basic principles of cost-benefit analysis and a listing of the economic costs of alcoholism, a case study was developed as an heuristic device to illustrate the algorithm and the potential utility of cost-benefit analysis, particularly when used in conjunction with sensitivity analysis, as a logical framework for the evaluation effort. | 139,372 | [
0.004603926,
-0.01848769,
-0.4145088,
0.2452827,
0.2341659,
-0.2024622,
-0.1156728,
-0.1605694,
0.1607284,
0.1272861,
0.02588295,
-0.1820679,
-0.145151,
0.2349776,
-0.1866297,
0.1070259,
-0.07838666,
0.3784744,
-0.3993278,
0.06353219,
0.08460297,
0.06634359,
0.1492786,
... |
Cell-mediated and humoral immune responses in mice. I. Necessary conditions for the detection of delayed-type hypersensitivity. | Footpad assay of delayed-type hypersensitivity to bovine serum albumin (BSA) was studied. Injection of saline solution of BSA (S-BSA) into footpads of sensitized mice resulted in the development of only the local Arthus-type reaction. Injection of alumprecipitated BSA (AP-BSA), however, was effective in generating both Arthus- and delayed-type reactions. The difference between S-BSA and AP-BSA in the ability of elicitation of delayed-type reaction may be attributable to the difference in the retention of these two forms of antigen in the footpad, since S-BSA was found to be eliminated much more rapidly from footpads than AP-BSA. | 139,378 | [
0.0009240375,
-0.3508965,
-0.2764298,
0.1689171,
0.3013135,
-0.07041804,
-0.04135753,
0.2223582,
-0.0995511,
-0.2056282,
0.161028,
-0.03235118,
0.07703686,
-0.1053423,
-0.1288504,
-0.2017009,
-0.1850778,
0.1674916,
-0.05008045,
0.1229379,
-0.1181387,
0.03203582,
0.0341840... |
Purification and some properties of the Glu- and Lys-human plasmin heavy chains. | The heavy polypeptide chains of human Glu-plasmin and human Lys-plasmin have been isolated in native solvents, after partial reduction and carboxymethylation of the corresponding plasmins. Two major forms of each heavy chain can be eluted, after adsorption to Sepharose/lysine, utilizing a gradient of epsilon-aminocaproic acid as the eluant. The elution profile of these heavy chains is practically identical to the elution behavior previously observed for human Glu- and Lys-plasminogen, and human Glu- and Lys-plasmin adsorbed to these columns. Sedimentation velocity analysis of the heavy chain of human Glu-plasmin, in the presence of epsilon-aminocaproic acid, demonstrated that a gross conformational alteration occurs in this peptide accompanying binding of this amino acid. A much smaller conformational alteration occurs under similar circumstances with the human Lys-plasmin heavy chain. We find that the NH2-terminal peptide released in the Glu-plasminogen to Lys-plasminogen and Glu-plasmin to Lys-plasmin conversions is also released in the Glu-plasmin heavy chain to Lys-plasmin heavy chain conversion. This reaction is catalyzed at a significant rate only by plasmin and not by urokinase. Finally, no strong interaction between streptokinase and the isolated plasmin heavy chains is observed. | 139,407 | [
-0.01699322,
0.2477702,
-0.31478,
0.2579126,
-0.07997093,
-0.05023323,
-0.05751254,
0.2471744,
0.3200463,
0.3502611,
-0.0754888,
0.1858582,
-0.08122321,
-0.1177156,
0.2453294,
0.3001361,
-0.2760501,
0.3798987,
0.2300638,
0.1520643,
0.4655493,
0.09057192,
-0.05421637,
-0... |
Orthoterminal correction of coexisting d-transposition of the great arteries, subpulmonary stenosis, and a complete form of atrioventricular canal. | This report outlines orthoterminal correction in an 8-year-old boy with coexisting d-transposition of the great arteries (d-TGA), subpulmonary stenosis, and a complete form of atrioventricular canal (CAVC). The repair consisted of covering the tricuspid valve and atrial part of the atrioventricular canal with a Dacron patch and inserting a valve-containing conduit between the then isolated right atrium and the pulmonary artery to create a venous outlet. The patient's exercise tolerance has improved remarkably. The arterial oxygen saturation has risen from 86 to 90 per cent. A plea is made to expand the indications for orthoterminal correction to patients in whom the severity of the heart malformation precludes application of well-established methods of surgical repair. | 139,507 | [
-0.08047159,
0.05135642,
-0.6174676,
0.0880545,
-0.1972319,
-0.176341,
-0.4399665,
-0.3530304,
-0.05130671,
-0.09855394,
-0.1592789,
0.1256388,
-0.1793638,
-0.2924734,
-0.1312191,
-0.09232184,
-0.5247822,
0.07775529,
0.0813101,
0.04121608,
-0.07100348,
0.1791548,
-0.30239... |
Hospitalization for laparoscopy. The exception rather than the rule. | After studying a series of patients who underwent laparoscopy, the authors found that they are currently hospitalizing only 6.6% of them. They found that more patients are hospitalized for diagnostic laparoscopy than for surgical laparoscopy because of the potential need for laparotomy. | 139,575 | [
0.0858037,
0.03300171,
-0.2695349,
0.01597804,
0.03452773,
-0.2079302,
-0.4026733,
-0.3196566,
-0.05669689,
-0.1196526,
0.129253,
0.03702055,
0.1414707,
-0.2573456,
-0.001041539,
0.09323266,
-0.2553875,
0.03910479,
-0.1926503,
-0.2240265,
-0.05289096,
-0.1226994,
-0.06701... |
Changes in the proportion of serum proteins induced by protein depletion in adapted and nonadapted rats. | Comparison of the dynamics of changes in the proportion of the serum protein during progressive protein depletion in rats specially adapted to this situation and animals exposed to it for the first time, led the authors to conclude that the regulation of proteosynthesis is largely adaptable, that it can be influenced by nutritional leads and that the organism probably stores information on past nutritional loads and makes use of it to help to overcome fresh insults. | 139,631 | [
-0.2014332,
-0.3007654,
0.2070042,
-0.09545302,
0.003771593,
-0.4146958,
0.03192887,
0.1411841,
0.1452441,
0.2533768,
0.2455731,
-0.1262777,
0.106542,
0.03684898,
-0.4717167,
-0.04010072,
-0.2855848,
-0.1904799,
-0.2596554,
0.2456572,
0.01881802,
0.1857232,
-0.1087684,
... |
[Scoliosis and congenital cardiopathies]. | The authors have followed up 26 children suffering from severe scoliosis associated with congenital heart disease up to the end of puberty. The curve was usually a very severe idiopathic scoliosis developing early and requring surgical treatment. Two types may be distinguished: I. Scoliosis without excessive surgical risk, in patients in whom the heart disease is not associated with cyanosis or where the heart condition has already been treated surgically and in which there are no clinical, radiological or electrical signs of cardiac failure. 2. Scoliosis with considerable surgical risk because of heart disease with cyanosis not treated surgically, or with signs of heart failure or pulmonary hypertension. In such cases, the surgical treatment of the scoliosis is likely to endanger life. | 139,660 | [
-0.1645272,
0.1734433,
-0.06117486,
-0.1902572,
-0.07348023,
-0.2911645,
-0.1938979,
-0.2117132,
-0.3093945,
0.003197195,
0.3533549,
0.3720942,
-0.1665315,
-0.575152,
-0.4425738,
-0.2758655,
-0.2420428,
0.07905461,
0.1872343,
-0.06374191,
0.5583262,
0.06306046,
-0.0369020... |
On the lympho-epithelial relationships in the human oviduct. | In order to study the lympho-epithelial relationships in human oviducts, lymphocytes situated in the epithelium were counted and the values found were correlated with differing physiological and pathological changes. In this process the following observations were made: 1. In the secretory phase there are significantly more lymphocytes to be found in the epithelium than during the proliferative phase. 2. In nonspecific purulent salpingitis the number of lymphocytes is not significantly different from that in a normal proliferative phase. 3. The values for fallopian pregnancy are significantly lower than those of a normal secretory phase and do not differ significantly from those of a normal proliferative phase. 4. The values observed for the reproductive period are significantly higher than those found postmenopausally. Examination by electron-microscopy showed that the lymphocytes in the epithelium of the uterine tube are intercellular. Pointers towards degenerative changes were not seen. It is presumed that the number of lymphocytes appearing in the epithelium of the fallopian tube depends on the hormonal activity of the ovaries. | 139,749 | [
0.1268498,
-0.1759456,
-0.1131494,
-0.05147202,
-0.2210481,
-0.4367101,
0.204802,
0.2172327,
0.296625,
0.2792701,
0.1012154,
-0.1577899,
-0.1898686,
0.1009528,
-0.3329728,
-0.2541809,
-0.2048946,
-0.01864589,
0.05255476,
0.1191968,
0.2502989,
0.2323263,
0.04805533,
0.15... |
Further compounds from the pedal gland of the bontebok (Damaliscus dorcas dorcas). | The identification of four further major constituents of the pedal gland exudate of the bontebok, Damaliscus dorcas dorcas, viz. alpha-terpineol, 2-n-heptylpyridine, m-cresol and (A)-6-dodecen-4-olide and the investigation of the stereochemistry of the double bond in (Z)-6-dodecen-4-olide by means of iterative computer analysis are described. An improved synthesis of this compound is outlined. | 139,777 | [
-0.000006451629,
-0.045944,
-0.1014058,
-0.1879792,
0.05272818,
-0.3648473,
-0.2614824,
0.06194932,
0.07825323,
-0.1431143,
-0.04794918,
-0.2139081,
0.1469914,
-0.1649351,
-0.4744285,
0.09101938,
-0.3630517,
0.2399771,
0.22953,
-0.06517818,
0.1480521,
-0.2025696,
-0.20873... |
Histochemical studies of striated muscle after temporary ischemia in the rat. | Temporary ischaemia of the hind linb of the rat was produced using a tourniquet with controlled pressure. Changes in the muscle seen after Gomori trichrome staining and after histochemical reactions for NADHdiaphorase, ATPases and phosphorylase were correlated with the duration of the ischaemia and the time of recovery. Histopathological changes were seen first after 2 h of ischaemia and increased thereafter; necrosis of most of the muscles occurred between 4 and 6 h of ischaemia. Necrosis and phagocytosis of muscle fibres and later the amount of regenerating fibres with characteristic histochemical staining properties increased linearly with increasing duration of ischaemia. Even after the most severe lesions of seemingly total necrosis of the muscle after 6 h of ischaemia most of the muscle regenerated within 18 days. Morphological alterations such as variation in fibre size, split fibres and central nuclei were still observed 226 days after ischaemia at which time the follow-up was terminated. Fibre type grouping was seen first after 45-90 days subsequent to 4 and 6 h ischaemia after incubation for ATPase, indicating concomitant neurogenic lesion in addition to the direct ischaemia of the muscle fibres. | 139,818 | [
0.2382327,
-0.02064492,
-0.1981295,
0.4215726,
-0.05647213,
-0.6184105,
0.05118634,
-0.1066309,
0.0665283,
-0.1308537,
-0.08971284,
0.05405363,
0.01889896,
-0.03640453,
-0.3379194,
-0.05548282,
-0.2020728,
-0.05765095,
-0.1785976,
0.2263832,
0.06194322,
0.1952138,
-0.0195... |
Down's syndrome and autoimmunity. | A patient with Down's syndrome (DS) with multiple autoimmune phenomena is described. She suffered from hypothroidism, a celiac-like enteropathy and hemolytic anemia, and displayed cellular immunity directed against peripheral nerve antigen and basic myeloprotein and serum autoantibodies to many other tissue antigens. Her mother did not suffer from any overt autoimmune disease, but similar autoantibodies were found in her serum. It is suggested that DS resembles other autoimmune diseases, especially that which occurs in the NZB mice. The resemblance is based on the assumption that in both cases genetic and/or viral factors cause T-cell dysfunction which leads on the one hand to increased susceptibility to infections and leukemias, to autoimmune phenomena and to depressed cellular immunity, and on the other hand to increased B--cell reactivity. | 139,824 | [
-0.1237104,
-0.4168949,
-0.1519512,
-0.2709474,
0.04373224,
-0.1038305,
-0.0895479,
0.318702,
-0.06946308,
0.3011948,
0.1188773,
0.6645817,
0.1604291,
-0.04385274,
-0.1363242,
-0.2468013,
-0.2787271,
-0.02372224,
-0.2217357,
0.09297239,
-0.04954319,
0.2471385,
-0.1603534,... |
Abnormal myocardial fluid retention as an early manifestation of ischemic injury. | Fifty-seven isolated, blood perfused, continuously weighed canine hearts have been utilized to study the development of abnormal myocardial fluid retention during early myocardial ischemic injury. Inflatable balloon catheters were positioned around the left anterior descending coronary arteries (LAD) of 54 hearts or the proximal left circumflex coronary arteries of three hearts for study of the following intervals of coronary occlusion: a) 10 minutes followed by 20 minutes of reflow, b) 40 minutes followed by either no reflow or by 20 minutes of reflow, and c) 60 minutes without reflow. After 60 minutes of fixed coronary occlusion, histologic and ultrastructural examination revealed mild swelling of many ischemic cardiac muscle cells in the absence of interstitial edema, cardiac weight gain, and obvious structural defects in cell membrane integrity. After 40 minutes of coronary occlusion and 20 minutes of reflow, significant cardiac weight gain occurred in association with characteristic alterations in the ischemic region, including widespread interstitial edema and focal vascular congestion and hemorrhage and swelling of cardiac muscle cells. Focal structural defects in cell membrane integrity were also noted. The development of abnormal myocardial fluid retention after 40 minutes of LAD occlusion occurred in association with a significant reduction in sodium-potassium-ATPase activity in the ischemic area, but with no significant alteration in either creatine phosphokinase or citrate synthase activity in the same region. Despite the abnormal myocardial fluid retention in these hearts, it was possible pharmacologically to vasodilate coronary vessels with adenosine and nitroglycerin infusion to maintain a consistently high coronary flow following release of the coronary occlusion after 40 minutes and to even exceed initial hyperemic flow values following release of the occlusion when adenosine and nitroglycerin infusion was delayed until 15 minutes after reflow. Thus, the data indicate that impaired cell volume regulation and interstitial fluid accumulation and focal structural defects in cell membrane integrity are early manifestations of ischemic injury followed by reflow, but fail to establish a major role for the abnormal fluid retention in altering coronary blood flow prior to the development of extensive myocardial necrosis. In contrast, fixed coronary occlusion for 60 minutes results in mild intracellular swelling but no significant interstitial edema and no obvious structural defects in cell membrane integrity. | 139,829 | [
-0.1815956,
0.3711741,
-0.3450162,
-0.1848553,
-0.02500555,
-0.2339988,
0.1802575,
-0.1040285,
-0.1935045,
0.0203548,
0.1088715,
0.2618979,
-0.281236,
0.1481527,
-0.04252429,
0.1567618,
-0.1655062,
0.006561732,
0.1238385,
-0.05479741,
0.3226367,
-0.1138561,
0.07422011,
... |
The clinico-epidemiological profile of guinea worm in the Ibadan district of Nigeria. | An epidemiological survey of guinea worm infection was carried out in 17 rural village communities north of Ibadan in western Nigeria from 1971 to 1975. The incidence was 13.5% in a population of 8,200. The sexes were equally affected although there were significantly more cases amongst boys than girls. A majority of the worms emerged from the lower limbs and the incidence of complications and disability was higher in those with lesions around the ankles and feet. The average duration of incapacity from effective work among this predominantly rural population was 100 days. A case is made for a concerted effort to eradicate this easily prevented disease by the provision of wholesome potable water to all persons. | 139,831 | [
-0.1144438,
0.06805968,
0.1070239,
-0.05292195,
-0.275284,
-0.2265923,
-0.2596198,
-0.2397682,
-0.07178216,
-0.366989,
0.113111,
-0.01395316,
0.101874,
-0.01115086,
-0.08087631,
-0.08413731,
-0.1472733,
0.2002302,
0.05960901,
0.1067078,
0.04964485,
0.4101428,
-0.02755668,... |
[Lymphocyte impairment in aging]. | In vitro stimulation of peripheral blood lymphocytes by T mitogens and in MLC is reduced in aging. As the number of T lymphocytes and their antigenic power are in the normal range, a functional T-cell defect is anticipated in high age. | 139,841 | [
-0.02371907,
-0.36558,
-0.2191447,
-0.08414233,
0.2714812,
-0.08506543,
-0.1732624,
0.3224899,
-0.2486878,
0.06120636,
-0.07057035,
0.3307247,
0.148803,
0.1559335,
-0.680828,
-0.2461738,
-0.377746,
0.1212174,
-0.065775,
0.4049035,
0.6640877,
0.1862795,
0.06486754,
-0.11... |
Acne fulminans: investigation of acute febrile ulcerative acne. | Eight cases of acne fulminans (acute febrile ulcerative acne) are compared with 13 previously reported cases. This rare disorder of male teenage patients is characterized by the sudden appearance of highly inflammatory, tender, ulcerative and crusted lesions on the back, chest, and face: it is one of the most scarring acute dermatologic disorders of young patients. At the onset of the disease, all patients showed febrile temperatures and marked leukocytosis; other systemic symptoms occurred with varying frequency. Polyarthralgia was noted in half of our series. Investigations of potential trigger mechanisms (eg, septisemia due to Corynebacterium acnes, immunologic defects, altered polymorphonuclear leukocyte function, Shwartzman phenomenon) did not yield any clues regarding the pathogenesis of this disease. Clinical and laboratory findings suggest that acne fulminans is not directly related to acne conglobata. | 139,850 | [
0.007695733,
-0.4864311,
-0.1704171,
0.2177152,
-0.0006641749,
-0.2141817,
-0.2777994,
-0.1131801,
0.1682305,
0.1202414,
-0.1664574,
-0.09603903,
0.1973904,
-0.1568365,
0.2563876,
-0.2734774,
0.1271747,
-0.1921958,
0.2967521,
0.1127058,
0.2715487,
0.4421527,
-0.0875828,
... |
Hidrotic ectodermal dysplasia: study of a large Chinese pedigree. | Hidrotic ectodermaldysplasia was found, to our knowledge, for the first time in a Chinese family in Malaysia, and it affected 15 members in five generations. The disease, which is transmitted as a non-sex-linked autosomal dominant trait, presumably originated from southern China. All 15 members had the typical nail, hair, and skin lesions, and we observed three different types of nail defects. Scalp alopeica was more extensive in the female members while keratoderma of the palms and soles was more notable in the male members. The nail and skin lesions also became severer with age. Except for the infectious eczematoid dermatitis present in the propositus, none had other skin or systemic disorders. All were relatively healthy and had normal life expectancies; | 139,851 | [
0.29158,
-0.08672501,
0.3315354,
0.1655936,
-0.08592323,
-0.101798,
-0.1022284,
-0.4819935,
-0.1508317,
0.06394475,
0.1410805,
0.1678187,
-0.0616855,
0.363524,
-0.03146364,
-0.148762,
-0.2133614,
0.1368539,
-0.05156067,
-0.5196735,
0.3193783,
0.4142847,
-0.2189375,
0.17... |
Molecular conformation of sodium heparan sulphate in the condensed phase. | By using the X-ray-diffraction results reported previously for sodium heparan sulphate, a twofold helical conformation with an axially projected disaccharide repeat (h) equal to 0.93 nm has been examined in detail. On the basis of a repeating sequence of 1,4-alpha-D-glucosamine and 1,4-beta-D-glucuronic acid, trial and stereochemically feasible molecular models were computer-generated. An optimum twofold helical conformation is proposed, incorporating stabilizing intra-chain hydrogen bonds across both glycosidic linkages. | 139,888 | [
-0.1010695,
0.2252131,
-0.3217277,
0.08910952,
0.1558979,
-0.2509825,
-0.2201362,
-0.01168542,
0.05683954,
0.1829666,
0.05332724,
-0.2003068,
-0.08986042,
-0.08215525,
-0.2906234,
0.02398314,
-0.0966897,
0.2866915,
0.06968214,
-0.0507087,
0.515274,
0.1322328,
-0.03271222,... |
Serum zinc and retinol-binding protein in acne. | The serum levels of zinc and retinol-binding protein (RBP) have been determined in 173 patients with acne and compared with those of a control group. The RBP is a specific transport protein and its level in plasma reflects the amount of vitamin A available to the tissues. Patients with severe acne were found to have lower levels of RBP than either patients with mild acne or healthy subjects of the same age. In the case of males with severe acne, the mean serum zinc level was significantly lower than that of the control group. No such difference was observed for girls. The observed condition of low levels of zinc and vitamin A in the serum of patients with severe acne may provide a rationale for the clinically good effect of oral zinc treatment. | 139,912 | [
-0.01973702,
-0.1165852,
-0.2219934,
-0.4537065,
-0.151486,
-0.3139181,
-0.4063917,
0.2006394,
-0.1404603,
0.1078197,
0.408798,
0.4779451,
-0.007092165,
-0.07971511,
-0.03625907,
0.05652279,
-0.08936993,
-0.1282533,
-0.1710684,
-0.2932426,
0.03940094,
0.2872652,
-0.290239... |
[Study of the interaction of Na+ and K+-ATPase of erythrocytes with ouabain. Effect of acetyl phosphate and p-nitrophenyl phosphate]. | Effects of ATP, acetyl phosphate (AcP) and p-nitrophenyl phosphate (p-NPP) on the inhibition of the Na+, K+-ATPase activity were studied. ATP, AcP and p-NPP were found to facilitate the ouabain-induced inhibition of the enzyme activity only after the injection of these phosphorylyzing agents into the erythrocyte ghosts. Inside the ghosts Na+ ions enhanced the effects of the phosphorylyzing agents. K+ ions in the environment removed the stimulating effects of ATP, AcP and p-NPP on the ouabain-induced inhibition of Na+, K+-ATPase activity. It is concluded that the sites of AcP and p-NPP hydrolysis as well as the active center for ATP are localized on the inner surface of the cell membrane. | 139,945 | [
-0.1018967,
0.0399842,
-0.1141391,
0.2440743,
0.01996485,
0.1704346,
-0.0080765,
0.4218965,
-0.08225413,
0.2622307,
0.220169,
0.2754362,
-0.09788968,
-0.1982114,
-0.4589662,
-0.06047689,
-0.5834521,
0.100096,
-0.01468341,
0.1549447,
0.5695034,
-0.07237177,
-0.06405229,
... |
Isolation and partial characterization of plasma membranes bearing human fetal-associated antigens. | A rabbit antiserum to first-trimester human fetal tissue had greater reactivity in complement fixation and saturation binding assays with fetal tissues than with both a pool of normal adult lung, liver, and kidney and pools of the individual organs. This anti-fetal membrane reactivity was only partially inhibited by carcinoembryonic antigen. The serum still reacted strongly with human fetal and tumor cells after rendering it specific for plasma membrane components by adsorption to and elution from intact human fetal tissue culture cells. This plasma membrane-specific serum was then used to monitor the purification of the fetal membrane-associated antigens. The fetal antigens copurified with the putative plasma membrane enzymatic markers 5'-nucleotidase and Mg2+-adenosinetriphosphatase through differential and density gradient centrifugation. Insulin-binding activity only partially copurified with the antigenic activity. Little antigenic activity was found in nuclear and mitochondrial fractions. The isolation protocol gives fetal plasma membrane-associated antigens in approximately 50% yield with moderate purification. The sera and isolation procedures described should have general utility for the detection of human oncofetal antigens. | 140,004 | [
-0.3575159,
-0.02683878,
-0.1679869,
-0.1381706,
0.05965575,
-0.1915185,
-0.521313,
0.2718051,
0.1978264,
0.1933755,
-0.1672168,
-0.01554758,
0.3677128,
-0.1943052,
-0.2721867,
0.0449337,
-0.4669433,
-0.262731,
-0.09204316,
0.4578638,
0.2356068,
0.1074641,
-0.3338208,
0... |
[Effect of psychotropic preparations on the activity of transport ATPase of rabbit skeletal muscle sarcoplasmic reticulum]. | The effect exerted by 5 groups of psychotropic agents on b activity of Ca, Mg-dependent ATP-ase of the sarcoplasmatic reticulum in the skeletal muscles of the rabbit and the transport of Ca2+ were looked into. The most profound inhibitory effect was found to be displayed by neuroleptics--phenothiazine derivatives with the piperazine ring in the side chain. Neuroleptics-butyrophenones produced a much less marked inhibitory action. Tricyclic antidepressants noticeably reduced the activity of the enzyme, while MAO inhibitors proved little effective in this respect. Tranquilizers--benzodiazepine derivatives--displayed a moderate inhibitory influence, while trioxazine turned out to be little active. The stimulants caffein, pentylene tetrazol (corazol), as well as high concentrations of lithium salts raised, whereas their low concentrations suppressed the activity of the enzyme. The inhibitory effect of psychotropic agents increased by as much s 11/2--2 times with regard to the enzyme preliminarily activated through incubation with ATP. | 140,062 | [
-0.09914991,
0.2608615,
-0.07685544,
0.2500453,
0.02197021,
-0.08990411,
-0.5712588,
-0.1100727,
-0.3120907,
0.1634507,
0.06438161,
0.2552406,
0.495302,
0.3278617,
-0.7238665,
0.1302103,
-0.5956997,
-0.08731414,
-0.1509201,
0.5210857,
0.6872107,
0.08518613,
-0.08543392,
... |
[The efficacy of the angiographical method in the evaluation of left ventricular muscle mass in the dog (author's transl)]. | In nine closed chest mongrel dogs 42 left ventricular cineangiographies for determination of left ventricular muscle mass were carried out. Buetterich's method was verified and a different procedure of calculation of myocardial wall thickens was proposed. By this method a better correlation line between calculated and weighed left muscle mass and a lower standard error of estimate is obtained. | 140,092 | [
0.06933951,
0.2135345,
-0.02395061,
-0.4906964,
0.368338,
-0.1466858,
-0.06695154,
-0.03996033,
0.05319228,
0.09430997,
-0.00797645,
0.06458589,
-0.01762362,
-0.1258895,
-0.7267619,
-0.0657078,
-0.3668455,
0.2744087,
0.06028912,
0.1816189,
0.3696225,
-0.1116439,
-0.073982... |
Sidedness of (sodium, potassium)-adenosine triphosphate of inside-out red cell membrane vesicles. Interactions with potassium. | Inside-out membrane vesicles of human red cells, prepared according to the method of Steck et al. (1970) Science 168, 255-257) have sufficiently low cation permeability to allow the examination of the side-specific interactions of ligands with the asymmetric sodium pump complex. In accordance with the known properties of the pump in intact cells the following results were observed: (a) ATP-dependent sodium influx and (b) maximal (sodium, potassium)-ATPase with K+ present inside the vesicles with larger than or equal to 20 micronM ATP. With much lower [ATP], K+ inhibited sodium-activated ATPase. K+ was inhibitory at either surface. Inhibition was different on the two sides since cytoplasmic (extravesicular) Na+ counteracted inhibition by cytoplasmic (extravesicular) K+ but not inhibition by K+ at the plasma or external membrane surface, i.e. intravesicular K+. A decrease in the steady state level of the phosphenzyme intermediate of sodium-activated ATPase was caused also by K+ at either surface. The effect of cytoplasmic K+ is compatible with its competitive inhibition of activation of phosphorylation of the enzyme by cytoplasmic Na+. At 37 degrees, the inhibitory effect of external K+ is due to interaction with the phosphoenzyme to form a stable complex of K+ with the dephosphenzyme resulting in a decreased overall reaction rate but increased turnover of the phosphoenzyme (E-P + K leads to EK + Pi). At 0 degree, external K+ inhibits by interacting with the unphosphorylated enzyme to form an occluded enzyme-K complex. This results in a decreased overall rate but relatively small change in apparent turnover of the phosphoenzyme. At 0 degree, but not at 37 degrees, external Na+ counteracted the inhibitory effects of external K+. | 140,167 | [
-0.3129633,
0.1411338,
-0.3740177,
-0.0920722,
0.1240156,
-0.04373656,
0.04770205,
0.3559288,
0.2038285,
0.1546884,
0.2229479,
0.3163102,
-0.06979294,
0.09119745,
-0.4063205,
-0.2418732,
-0.4796707,
-0.007632512,
-0.03493424,
-0.07996055,
0.5743603,
0.08169731,
-0.0485154... |
Interactions between segmented polyurethane surfaces and the plasma protein fibrinogen. | Surfaces of a segmented polyurethane were varied by casting on poly(ethylene terephthalate) (PET) and glass substrates, and were characterized through infrared-attenuated total-reflection spectroscopy (ATR). Surfaces cast on glass substrates showed a higher content of polyether segments, whereas those cast on PET contained a higher relative concentration of aromatic segments. Adsorption, and possible conformational changes of fibrinogen, were found to be more substantial on polymer surfaces having a higher content of polyether segments. It is concluded that the relatively good blood compatibility of segmented polyurethanes is partly due to the presence of peptide-like bonds on aromatic segments. | 140,169 | [
-0.06357547,
0.5249966,
0.03129687,
-0.04820724,
0.2296709,
-0.1643193,
0.1394943,
-0.05841408,
0.2787091,
0.576964,
0.1319101,
-0.08822666,
0.04502455,
-0.01973284,
-0.3587635,
0.1228784,
-0.4461286,
0.2364009,
-0.1047319,
0.2675331,
-0.05834659,
0.1901139,
-0.05905651,
... |
Suppression of immune complex-induced inflammation by the chemotactic factor inactivator. | Small amounts (10(-10) mol) of purified human chemotactic factor inactivator (CFI) suppress leukocytic infiltration, permeability changes, and hemorrhage associated with acute immune complex-induced injury in rats. The reversed passive dermal Arthus reaction and acute immune complex-induced alveolitis in rats have served as the model systems of inflammation. The mechanism of inhibition does not appear to relate to interference with formation and deposition of immune complexes, or with fixation of complement in vitro or iv vivo. Human CFI inhibits in vitro the chemotactic activity generated in complement-activated rat serum. The inhibitory effects of human CFI are not seen if it is first heat inactivated. The data provide the first direct support for the conclusion that CFI has anti-inflammatory activity. | 140,184 | [
-0.1591413,
0.07397613,
-0.07548416,
-0.1678507,
0.2468788,
0.0465832,
0.07815178,
0.2440525,
0.09626246,
-0.0314314,
0.08405921,
-0.1485597,
0.2226748,
-0.1260146,
-0.01502068,
-0.1853498,
-0.4224178,
-0.1799485,
-0.1830212,
0.4238788,
-0.1351059,
-0.1010373,
-0.2656176,... |
Distinctive functional properties of human blood L lymphocytes: a comparison with T lymphocytes, B lymphocytes, and monocytes. | Human blood lymphocytes with high affinity Fc receptors have been operationally named L lymphocytes because of membrane-labile IgG markers. L lymphocytes lack membrane-incorporated immunoglobulin and do not form rosettes with sheep red blood cells coated with IgM antibody and mouse complement. These lymphocytes are capable of binding IgG in normal human serum at 4 degrees C and will form rosettes with human lymphocytes coated with Ripley IgG. In this study, functional in vitro properties of isolated L lymphocytes were compared with T lymphocytes, B lymphocytes, and monocytes. To obtain these mononuclear populations, first, plastic adherent monocytes were harvested. T lymphocytes were then isolated by centrifugation of E rosette-forming cells, and other rosetting techniques were employed to isolate L and B lymphocytes by negative selection. The functional properties of L lumphocytes were completely unlike those of T cells, B cells, or monocytes. L lymphocytes did not proliferate in response to mitogens, soluble antigens, or cell surface antigens. Moreover, this population could not replace monocytes in helping T lymphocytes respond to concanavalin A and pokeweed mitogen. Once T cells were supplemented with monocytes, however, the addition of L lymphocytes to the culture greatly enhanced the T lymphocytes proliferative response to phytohemagglutinin, concanavalinA, purified protein derivative (PPD), and streptokinase/streptodornase. L lymphocytes were not a subset of B cells. They did not spontaneously develop surface Ig in culture, and pokeweek mitogen could not induce them to transform and generate cytoplasmic Ig detectable by immunofluorescence. Mixtures of B cells and T cells responded to pokeweed mitogen better than do T cells alone. In contrast, enhanced reactivity with L and T cell combinations was not observed. Another sharp difference between these two populations was the stimulator capacity of each in mixed lymphocyte culture. When B and L lymphocytes were carefully monocyte-depleted, only B cells were effective stimulators of autologous and allogeneic lymphocytes. In comparison with T cells, B cells, and monocytes, L lymphocytes were the only effective killers of human blood lymphocytes sensitized with IgG. L lymphocytes, then, have cytotoxic potential, but cannot proliferate in response to various stimulants or become antibody-producing cells. These findings suggest that L lymphocytes comprise a third lymphocyte population. | 140,193 | [
0.1259967,
-0.1536437,
-0.01131209,
0.1148975,
0.2740176,
-0.2320584,
-0.1214587,
0.2622005,
0.05031208,
0.1486735,
0.2522858,
0.1654239,
0.06584597,
0.2620783,
-0.02006557,
-0.1558112,
-0.5180892,
-0.3005709,
0.2479457,
0.4237677,
0.3313884,
0.07519196,
-0.3842219,
-0.... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.