title stringlengths 0 901 | abstract stringlengths 3 9.89k | PMID int64 22 25.3M | embedding listlengths 768 768 |
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Dispersion and cultivation of renal cells after short-term storage of kidneys. | A new method for the preparation of cell suspensions from human newborn kidneys is described. It involves the use of a mixture of trypsin-ethylenediaminetetraacetic acid and collagenase. The cell yields obtained after tissue dispersion by this method were significantly greater than those obtained after dispersion with ... | 170,318 | [
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The requirement for macrophage-lymphocyte interaction in T lymphocyte proliferation induced by generation of aldehydes on cell membranes. | Guinea pig T lymphocyte proliferation induced by sodium periodate (NaIO4) or neuraminidase-galactose oxidase (NG) occurs when lymphocytes and macrophages are cultured together after treatment of either purified T lymphocytes or macrophages with these agents. Regardless of which cell initially bears the modified surface... | 170,338 | [
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Cell-mediated immunity to Friend virus-induced leukemia. I. Modification of 125IUdR release cytotoxicity assay for use with suspension target cells. | Cell-mediated cytotoxic reactivity of C57BL/6 mice against syngeneic FBL-3 cells, a Friend virus-induced leukemia, was measured by the 125IUdR release assay with tumor target cells in suspension. The tests could be performed either with Linbro tissue culture plates(16-mm wells) or with Microtest II plates (6-mm wells).... | 170,343 | [
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Enzymic activity of purified plasma membranes from the yeast and mycelial forms of Candida albicans. | Plasma membranes were isolated from the yeast and mycelial forms of Candida albicans as described previously (Marriott, 1975) and examined for the presence of several enzymes. Measurement of specific activities showed enrichment of Mg2+-dependent and Ma+/K+-stimulated Mg2+-dependent adenosine triphosphatase and mannan ... | 170,363 | [
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Time-dependent image quality using 99mTc-pyrophosphate. | Technetium-99m-labeled pyrophosphate has proved to be a useful skeletal-imaging agent. In this study, specific areas of the skeleton were imaged at times ranging from 1/2 to 6 1/2 hr after injection of 99mTc-pyrophosphate. Count ratios between abnormal and normal bone with respect to adjacent soft tissue were obtained ... | 170,380 | [
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Comparative ultrastructural study of four reticuloendothelias viruses. | The morphology and development of four members of the reticuloendotheliosis virus group were studied by transmission electron microscopy. Virions of duck spleen necrosis virus, duck infectious anemia virus, chicken syncytial virus, and reticuloendotheliosis virus strain T are sperical with a diameter of approximately 1... | 170,410 | [
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Synaptic organization in teleost spinal motoneurons. | Glass microelectrodes were inserted into motoneurons innervating pectoral fin muscles to record action and synaptic potentials, evoked by electrical stimulation of ventral and dorsal roots, and the medulla oblongata. Ventral root stimulation evoked a small depolarizing response which had properties compatible with thos... | 170,446 | [
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Failure of neutral-red photodynamic inactivation in recurrent herpes simplex virus infections. | Because photodynamic inactivation of herpes simplex virus infections may not be free of hazard, the efficacy of photodynamic inactivation with neutral red and light was evaluated in a placebo-controlled study of 170 episodes of recurrent herpes simplex virus infection in 96 patients. The preparation of neutral red that... | 170,521 | [
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Mode of action of sodium nitroprusside on vascular smooth muscle. | 1. Sodium nitroprusside is a potent relaxant of smooth muscles with a predominantly tonic response, e.g. rat aorta contracted by noradrenaline, angiotensin II, Phe2-Lys8-vasopressin, BaC1(2), or KC1, and guinea-pig tracheal smooth muscle contracted by carbachol. 2. Smooth muscle preparations from the splanchnic region ... | 170,545 | [
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Morphological changes of astrocyte-like cells induced by serum beta-lipoprotein in brain cell culture. | When added to brain cell cultures of newborn rats, serum and, in particular, its beta-lipoprotein fraction caused significant morphological transformations of astrocyte-like cells present in the culture. The changes were instantaneous as they appeared within 1 min after addition of beta-lipoprotein and they were charac... | 170,552 | [
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Electron microscopic demonstration of hormone-producing metastatic carcinoma of the choroid from the bronchus. | Clinical, laboratory and pathological findings of a patient in bronchial carcinoma with choroidal metastasis were presented. X-ray examination of the chest suggested the tumor shadow in the posterior segmental bronchus of the right upper lobe of the lung (r-B2b), while funduscopy and fluorescein angiography revealed th... | 170,572 | [
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[Concentration of 99mTc-tin-phosphate complexes in soft tissues]. | The concentration of 99mTc-pyrophosphate was determined in the lower extremities of rabbits (normal, abacterial and bacterial affected soft tissues), in osteoarthritis of the hip joint (capsule and muscle) as well as in knee joint effusions. Compared with the 85Sr-concentration, reflecting the calcification capacity, c... | 170,592 | [
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Antibody titers by mixed agglutination to varicella-zoster, herpes simplex and vaccinia viruses in patients with multiple sclerosis. | Antibody titers to varicella-zoster (V-Z), herpes simplex (HSV) and vaccinia viruses were determined in sera collected in Buffalo, NY, from patients with multiple sclerosis (MS), patients with other diseases, and normal individuals. The mixed agglutination test employing cell cultures infected with one of the above vir... | 170,625 | [
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Growth inhibitory and arginase activities in liver and hepatoma extracts. | The growth-inhibitory activity against H.Ep. No 2 cells observed in phosphate buffer extracts of rat liver appears to be due to the enzyme, arginase. This conclusion is based on the findings that (1) extracts of hepatomas containing lower levels of the enzyme also contain less inhibitory activity; (2) partial purificat... | 170,626 | [
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Non-healing granuloma and the nervous system. | Three cases of non-healing granuloma with neurological complications are described. One case suffered from Stewart's form of the disease and two from Wegener's variety. The literature is extensively reviewed and the incidence and manner of neurological involvement in 374 cases is discussed. We suggest a classification ... | 170,638 | [
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Selective carotid angiography in the diagnosis of fibrous histiocytoma. A case report. | Selective carotid angiography is of value in the diagnosis of an orbital fibrous histiocytoma, an uncommon tumor with a high local recurrence rate and frequent distant metastases. The angiogram shows the physical extent of the tumor and also suggests its general microscopic anatomy. Comparison is made with similar angi... | 170,643 | [
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Regression line analysis for five antibiotics with strains of Clostridium species. | The susceptibility of 90 strains of clostridia to 5 antibacterials was determined by the agar plate dilution method and the disc diffusion test. The correlations between minimum inhibitory concentration and inhibition zone were quite acceptable for lincomycin, clindamycin, tetracycline and doxycycline to calculate regr... | 170,669 | [
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Bronchial cancer - a clinical and pathological study. I. Histopathology and metastases. | Among 16,394 necropsies during the 12-year period, 1958-1969 (representing about 60% of all persons who died in Malmö during this period, 747 cases of bronchial cancer were found in 744 individuals. There were considerable differences in frequency of different histologic types with regard to sex. Two main classes have ... | 170,673 | [
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Bronchial cancer - a clinical and pathological study. II. Frequency according to age and sex during a 12-year period. | A necropsy series of bronchial cancer combined with a retrospective clinical study comprising 747 cases of bronchial cancer from a defined population is described. A significant successive increase in the frequency of bronchial cancer during the 12-year period 1958-1969 was found. The increase during three successive 3... | 170,674 | [
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[Hypolipidemias]. | Hypolipidemias can be divided in primary, familial and hereditary forms and symptomatic forms which may accompany other diseases. The primary hypolipidemias (abetalipoproteinemia, hypobetalipoproteinemia and analphalipoproteinemia) are very rare. Severe hypolipidemia can be found in some peoples (e.g. the Masai). This ... | 170,675 | [
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Antibodies that impair insulin receptor binding in an unusual diabetic syndrome with severe insulin resistance. | Six patients with a unique form of diabetes associated with extreme insulin resistance have markedly reduced insulin binding to specific receptors on their circulating monocytes. When normal insulin receptors were exposed to serum or immunoglobulin fractions from three of these patients in vitro the specific binding de... | 170,678 | [
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Magnetotactic bacteria. | Bacteria with motility directed by the local geomagnetic field have been observed in marine sediments. These magnetotactic microorganisms possess flagella and contain novel structured particles, rich in iron, within intracytoplasmic membrane vesicles. Conceivably these particles impart to cells a magnetic moment. This ... | 170,679 | [
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Biological behavior of the primitive neuroectodermal tumors: significant supratentorial childhood gliomas. | Supratentorial gliomas in the pediatric age group at the University of Kentucky Medical Center accounted for 20% of all childhood brain tumors. Seventeen of the 20 children with these tumors, regardless of histologic type, mode of therapy, or other parameters died within five years after tissue diagnosis, and most were... | 170,697 | [
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[Structure of Candida tropicalis K-41 thermotolerant yeasts at various growing temperatures on a mineral medium with hydrocarbons]. | In the C. tropicalis K-41 cells fixed by glutaraldehyde and osmium most membranes consisted of two electron-transparent and one electron-dense layer. Morphologo-structural peculiarities of the cell fraction grown at 39 degrees testify to a certain disconnection of energetic and constructive metabolism, inhibition of sy... | 170,720 | [
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A study of the neurohumoral control of glycolysis in the mouse brain in vivo: role of noradrenaline and dopamine. | Intraventricularly injected noradrenaline, dopamine and isoprenaline increased glycolysis as shown by the decrease in the concentration of "free" glycogen and increase in the concentration of lactate. The effects of noradrenaline and isoprenaline were reduced in mice which had been pretreated with alpha-methyl-p-tyrosi... | 170,752 | [
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On the phospholipase activity in bovine seminal vesicles and its possible role in the regulation of the prostaglandin biosynthesis. | A phospholipid mixture was isolated from bovine vesicle tissue. This phospholipid preparation contained the following prostaglandin precursor fatty acids: 4.7 percent 5,8,11-eicosatrienoic acid, 9.1 percent 5,8,11,14-eicosatetraenoic acid and 0.9% 5,8,11,14,17-eicosapentaenoic acid. With this phospholipid preparation a... | 170,754 | [
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The possible utilization of nuclear pockets of lymphocytes in the diagnosis of enzootic bovine leucosis. | A comparative study of the presence of lymphocytosis and nuclear pockets in lymphocytes was carried out using blood samples from animals belonging to herds with bovine enzootic leucosis and from herds free from leucosis. It was found that there was a correlation between the presence of such nuclear projections in a cer... | 170,789 | [
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Studies in asymptomatic primary hyperlipidaemia. V. Peripheral circulation. | The occurrence of early signs of peripheral vascular disease (PVD) of the lower limbs has been studied with digital pulse plethysmography in 160 men and 123 women above the age of 35, who were all subjectively healthy but were found to have primary hyperlipoproteinaemia (HLP) at a health control centre. Quantitative an... | 170,797 | [
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0.04471523,
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-0.1091462,
-0.05895708,
0.4187633,
0.08061446,
0.3973089,
-0.1709564,
... |
Calcium pyrophosphate-induced pleurisy in rats: a new model of acute inflammation. | A model is described of acute inflammation in the pleural cavity of rats using calcium pyrophosphate as the irritant. This model would seem to simulate the pseudogout syndrome. It has been shown to be acute in onset, dominated by polymorphonuclear cells, complement independent. The advantage of the model is that volume... | 170,810 | [
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0.05754381,
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0.1016837,
0.1611922,
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Cytoplasmic inclusions of neurons in the monkey visual cortex (area 19). | Several unusual neuronal inclusions were found in certain cells of the rhesus monkey visual cortex (Area 19): 1. Filamentous bodies, present in the small stellate cells of layer IV, globoid, 0.3-0.6 mum in diameter, consisting of fine 50 A filaments in a hexagonal meshwork. These are often associated with the labyrinth... | 170,843 | [
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0.1679545,
0.2712804,
0.60734,
0.01003785,
... |
Re-evaluation of dosage and duration of action of d-tubocurarine in the pediatric age group. | A cumulative dose-response curve for d-tubocurarine based on body weight was determined for 44 infants and children 1 day to 7 years of age during halothane, nitrous oxide and oxygen anesthesia. Depression of thumb adduction was measured. Age difference did not affect the mean dose-effect response. Infants less than 10... | 170,845 | [
-0.2103542,
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0.4786817,
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0.2111547,
0.4192745,
0.2808422,
0.12183... |
Detection of animal viruses in coastal seawater and sediments. | Animal viruses, predominantly enteroviruses, were detected in shallow water at bottom depths and in clastic marine sediments. Viruses accumulated in sandy and slimy deposits of the sea bottom near the shore and could be easily released into water by means of simple mechanical shaking. | 170,859 | [
-0.1874366,
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0.1886317,
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0.01456055,
0.2975435,
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-0.077... |
[Basophilic adenoma in combination with a prolapse of adenohypophyseal tissue through the capsule]. | During the post-mortem examination of a 15-year patient who had died after the operation connected with incision of commissures a distorted form of the hypophysis was noted. Histological, histochemical and morphometric investigations showed that there was basophilic adenoma of adenohypophysis and prolapse of the tissue... | 170,895 | [
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0.448853,
0.2010771,
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... |
Malignant lymphoma resembling Burkitt's tumour in rhesus monkeys: (light- and electron microscopic studies). | Spontaneous tumours of the haematopoetic system have been only rarely observed in nonhuman primates. We had the opportunity to carry out light and electron microscopic investigations of three lymphomas in immature rhesus monkeys from our colony. All animals had tumours of both retroperitoneum and lymph nodes. The relat... | 170,906 | [
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0.124948,
0.... |
Thermography and rheumatic diseases. | The common factor in most of the rheumatic diseases is an arthritis. Radiometry and thermography have been shown to indicate and measure heat resulting from localised inflammation. In rheumatoid arthritis, juvenile arthritis, osteoarthrosis, gout and ankylosing spondylitis abnormal heat distribution has been recorded o... | 170,907 | [
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0.1643177,
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0.1878947,
0.5674888,
0.3729337,
-0.257772... |
Studies on sex-organ development. Isolation and characterization of an oestrogen receptor from chick Müllerian duct. | An oestradiol-binding macromolecule was observed in the left Müllerian duct of the 15-day female chick embryo. The embryonic receptor binds oestradiol with a high affinity and low capacity, having a Kd of 3.2 X 10(-9)M and a maximal number of sites of 5.45 fmol/10(6) cells in the left Müllerian duct. The receptor is pr... | 170,917 | [
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0.07876284,
0.6882846,
0.2896973,
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... |
[The mechanism of action of bencyclane on smooth musculature]. | Bencyclane (N-[3-(1-benzyl-cycloheptyloxy)-propyl]-N,N-dimethyl-amine-hydrogenfumarate, Fludilat), inhibits phosphodiesterase(PDE)-activity in vitro similarly to several other smooth muscle relaxants. Compared with papaverine this inhibitory effect of bencyclane on PDE is weak despite of its strong relaxant effect on s... | 170,943 | [
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0.3144852,
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... |
8-Substituted derivatives of adenosine 3',5'-cyclic phosphate require an unsubstituted 2'-hydroxyl group in the ribo configuration for biological activity. | Derivatives of adenosine 3',5'-cyclic phosphate (cAMP) with modifications in both the 2' and the 8 positions were synthesized and their enzymic activities as activators of cAMP-dependent protein kinase and as substrates for and inhibitors of cAMP phosphodiesterases were determined. Three types of derivatives were inves... | 170,958 | [
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0.009167706,
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0.1778992,
0.5458921,
0.1600429,
-0.235960... |
31 P nuclear magnetic resonance studies of the interaction of pyridine nucleotide coenzymes with dehydrogenases. | 31P nuclear magnetic resonance spectra of the pyrophosphate group in NAD+ and NADH were recorded in the presence of beef heart lactate dehydrogenase and rabbit muscle glyceraldehyde-3-phosphate dehydrogenase. At high lactate dehydrogenase concentrations (60 mg/ml), two NADH resonances are observed: a slowly exchanging ... | 170,965 | [
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0.4428303,
0.3815737,
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0.... |
Phosphonopyruvic acid: A probable precursor of phosphonic acids in cell-free preparation of Tetrahymena. | 1. In cell-free preparations of Tetrahymena, doubly labelled [32P]phosphoenol-[3-14C]pyruvate gives rise to 2-aminoethylphosphonate and 2-amino-3-phosphonopropionate, labelled with the two isotopes in the same ratio as the starting compound. The result is consistent with an intra-molecular rearrangement of phosphoenolp... | 170,979 | [
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0.2739855,
0.4399951,
0.4807709,
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A comparison of the interfacial interactions of the apoprotein from high density lipoprotein and beta-casein with phospholipids. | The conformations adopted by beta-casein and the total apoprotein from serum high density lipoprotein when spread at the air-water interface are compared; the monolayer data are consistent with the apoprotein being alpha-helical and the beta-casein being disordered with segments distributed in loops and trains. The pen... | 170,986 | [
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0.09955858,... |
Synthesis of viral proteins by the avian myeloblastosis viral core component. | Avian Myeloblastosis Viral (AMV) core component was isolated and shown to synthesize AMV proteins in vitro. This reaction was linearly dependent on viral core concentration, proceeded linearly with time, and was inhibited by puromycin and aurintricarboxylic acid. The proteins synthesized in vitro co-electrophoresed and... | 170,991 | [
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Plasma lipoproteins in rats with experimental biliary obstruction. I. A chemical study. | Acute biliary obstruction in the rat is associated with striking alterations of the plasma level and the physico-chemical properties of plasma lipoproteins. 1. The level of very low density lipproteins (VLDL) in plasma increases from 2 to 3 fold. The chemical composition of VLDL is characterized by a high content of ph... | 170,992 | [
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0... |
[Effect of catecholaminergic substances on the proconvulsive properties of the caudate nucleus]. | In the freely moving rats stimulants of catecholaminergic tramission (DOPA, apomorphine, d,1-amphetamine, and also their combination with disulfiram) reduced the proconvulsive properties of the caudate nucleus. Under the effect of these substances there occurred a shortening of the cortical electroencephalographic resp... | 171,015 | [
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The nitrofurans as sperm immobilising agents: their tissue toxicity and their clinical application. | Nitrofurantoin sodium and nitrofurazone in certain critical concentrations have the ability to cause sperm immobilisation. Intraoperative vas irrigation with these solutions in 24 patients have produced sterility in the immediate postoperative period. | 171,024 | [
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[Role of ACTH in regulation of aldosterone secretion]. | During the nycthemeral cycle, ACTH modulated the aldosterone production and increases plasmatic level at the end of night. The chronic stimulant effect of angiotensin II is a permissive factor of ACTH influence on aldosterone formation. | 171,034 | [
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... |
[Spike activity recorded at the level of optic tract in a primate, Papio papio]. | Spike activity has been recorded, in two young baboons, at the part of the optic tract (O.T.) localized between the chiasma and the lateral geniculate (L.G.) during REM and also slow wave sleep, preceding or not a REM period. These spikes, mono or biphasic, were of 60-200 ms duration and of 30-140 muV amplitude. They o... | 171,037 | [
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Preoperative irradiation of cancer of the lung: final report of a therapeutic trial. A collaborative study. | Between May, 1963 and December, 1966, 17 medical centers cooperated in two separate but integrated therapeutic trials of primary lung cancer. One study was of patients with lesions considered operable at the time of diagnosis, and the other of patients with initially inoperable cancer but who were considered potentiall... | 171,057 | [
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0.2946596,
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Diminished binding of thyroid-stimulating hormone in a transplantable rat thyroid tumor as a possible cause of hormone unresponsiveness. | The adenylate cyclase activity and the binding of 125I-labeled thyroid-stimulating hormone (TSH) of normal and tumor rat thyroid plasma membranes were compared. No significant difference in the basal and fluoride-sensitive adenylate cyclase activity between normal and tumor plasma membranes was observed. Thyroid plasma... | 171,062 | [
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0.5462446,
0.2198243,
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Some biochemical characteristics of rat liver and Morris hepatoma nuclei and nuclear membranes. | Nuclei prepared from host liver and from Morris hepatomas 7777 and 7800 have been compared with respect to some of their biochemical characteristics. Several criteria were used to ensure that liver and hepatoma nuclei were of equal purity. These criteria include equal specific activity ratios (homogenate nuclei) for se... | 171,064 | [
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0.06801574,
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0.236811,
0.005502751,
-0.05302827,... |
Nuclear secretory particles associated with the calyx cells of the ichneumonid parasitoid Campoletis sonorensis (Cameron). | The present study is an ultrastructural investigation of the calyx region of the ichneumonid endoparasitoid Campoletis sonorensis. It appears that synthesis of electron-dense secretory particles occurs within nuclei of calyx cells. The particles consist of an ovocylindrical electron-dense inner core and a surrounding u... | 171,071 | [
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0.6887752,
0.1595344,
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0.0... |
Thymus transplantation. Reconstitution of cellular immunity in a four-year-old patient with T-cell deficiency. | The case is reported of a 4-year-old girl affected with recurrent infections; anaemia, thrombocytopenia, haemorrhages and hepatosplenomegaly. Immunological investigations revealed a defect in cellular immunity related to the thymus-dependent system, hypergammaglobulinaemia (especially of class IgE), and very high titre... | 171,111 | [
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Hormone receptors, 5. Binding of glucagon and insulin to human circulating mononuclear cells in diabetes mellitus. | Preparations of human peripheral mononuclear leukocytes, containing over 85% lymphocytes, specifically bound glucagon and insulin. Binding of physiological concentrations of both hormones was seen to be substantially diminished when cells were derived from either adult-type diabetics or individuals with a unilateral ge... | 171,138 | [
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0.3996217,
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3,3',5'-Triiodothyronine (reverse T3) and 3,3',5-triiodothyronine (T3) in fetal and adult sheep: studies of metabolic clearance rates, production rates, serum binding, and thyroidal content relative to thyroxine. | To examine the mechanism(s) responsible for high serum concentration of 3,3',5'-triiodothyronine (reverse T3, rT3) and low serum concentration of 3,3',5-triiodothyronine (T3) in the fetus, we studied metabolic clearance rates (MCR) and production rates (PR) of rT3, T3, and thyroxine (T4) in adult nonpregnant sheep and ... | 171,141 | [
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0.3964257,
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Characterization of mouse ACTH in plasma and in extracts of pituitary and of adrenotropic pituitary tumor. | The predominant component of immunoreactive ACTH in the plasma of adrenalectomized normal mice and of mice bearing the adrenotropic mouse pituitary tumor, AtT-20, and in extracts of the normal mouse pituitary and pituitary tumor, has an elution volume on Sephadex G-50 gel filtration approximately midway between the voi... | 171,153 | [
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0.0769118,
0.02798844,
... |
Epileptogenic effects of several penicillins and penicillin-related compounds in rat neocortex. | Several penicillins and penicillin-related compounds were tested for their ability to produce epileptiform activity in rat neocortex. (1) Alterations in the side chain of penicillin G decreased epileptogenic capability in all the compounds tested in this study: Phenoxymethylpenicillin produced a primary focus but no mi... | 171,154 | [
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... |
Treatment of RSV-transformed "poorly" virogenic and non-virogenic mammalian cell lines with inhibitors of protein synthesis: failure to potentiate RSV rescue. | Treatment of the RSV-transformed "poorly" virogenic rat clones LW13-RsK4 and LW13-RsK4 R1 with cycloheximide or puromycin before fusion with chick embryo fibroblasts did not lead to RSV rescue. Neither was RSV rescued from the RSV-transformed non-virogenic mouse line RVP3 treated with 5-bromodeoxyuridine and cyclohexim... | 171,178 | [
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Changes of enzyme activities in spleen lymphocytes from tumor-bearing rats. | The induction of concomitant immunity was studied in Donryu strain rats with Yoshida ascites sarcoma cells. The changes of enzyme activity in spleen lymphocytes were also examined in normal and tumor-bearing rats. The concomitant immunity was detected 1 week after transplantation of tumor cells. Extended metastases wer... | 171,191 | [
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0... |
Age-dependent arginine phosphokinase activity changes in male vestigial and wild-type Drosophila melanogaster. | The activity of arginine phosphokinase, an important muscle enzyme in insects, was investigated with age in vestigial-winged and wild-type Drosophila melanogaster. Identical patterns of age-dependent activity changes were observed in the vestigial-winged flies as in the wild-type, even though vestigial-winged flies exh... | 171,197 | [
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... |
In vitro studies of calcitonin release in man. | The influence of various agents on calcitonin release from human thyroid was studied in vitro. Under the condition of this investigation, calcium, magnesium and phosphate did not stimulate calcitonin release from short-term incubated slices of human thyroid. However, pentagastrin and USP glucagon were potent stimulator... | 171,204 | [
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Purification and characterization of neuraminidase from Clostridium perfringens. | Clostridium perfringens cells were cultivated on a large scale using an automatic system. Neuraminidase secreted by the cells into the culture medium was purified 380 000-fold by: precipitation with ammonium sulfate between 50 and 85% saturation, filtration on Sephadex G-75, electrophoresis on polyacrylamide gel, and b... | 171,212 | [
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Prenatal diagnosis of I-cell disease. | A pregnancy from a family in risk of I-cell disease was monitored. The fetus was diagnosed as having I-cell disease based on the findings that (1) lysosomal enzyme activities except for acid phosphatase and alpha glucosidase were clearly elevated in amniotic fluid and were reduced in cultivated amniotic fluid cells, an... | 171,215 | [
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0.3679356,
0.01786296... |
Interactions between endogenous baboon type-C virus and oncogenic viruses. I. Syncytium induction and development of infectivity assay. | Cells releasing the endogenous baboon virus (BV) can interact with human KC cells containing the Rous sarcoma virus (RSV) genome, resulting in cell fusion and syncytium formation. This interaction has been utilized in the development of a sensitive infectivity assay for BV. The titration pattern is of a one-hit type, d... | 171,228 | [
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Studies in lipid histochemistry. XIII. The OPA (osmiumtetroxide-periodic acid-alpha-naphthylamine) method for the detection of apolar lipids. | A new procedure for the detection of apolar lipids is described. It is a modification of the OTAN method (Adams, 1959) using periodic acid which oxidatively removes lower osmium derivatives from polar sites only, leaving those in apolar lipids intact and demonstrable with alpha-naphthylamine. Control steps for the excl... | 171,245 | [
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Effect of bredinin and its aglycone on L5178Y cells. | The aglycone of the nucleoside antibiotic, bredinin, was as strongly cytotoxic to L5178Y cells as bredinin. The cytotoxic properties of the aglycone were very similar to those of bredinin and the minimum inhibitory concentrations of both were 10(-5)M. The growth inhibitory effects of both agents regardless of their con... | 171,246 | [
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Structural analyses of mammalian ribosomal ribonucleic acid and its precursors. Nucleotide sequence of ribosomal 5.8 S ribonucleic acid. | The nucleotide sequence of ribosomal 5.8 S RNA (also known as 7 S or 5.5 S rRNA) from Novikoff hepatoma ascites cells has been determined to be (see article). Estimations of the secondary structure based upon maximized base pairing and the fragments of partial ribonuclease digestion indicate that there may be five base... | 171,258 | [
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Identification of an essential lysine in porcine heart mitochondrial malate dehydrogenase. | The reversible inactivation of porcine heart mitochondrial malate dehydrogenase by pyridoxal 5'-phosphate yields an irreversible modification upon sodium borohydride reduction. A 200-fold molar excess of pyridoxal-5'-P over enzyme results in inactivation to the extent of 54%, and incorporation of 5.7 mol of inactivator... | 171,264 | [
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0... |
Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. | 1. Xanthine oxidase acting aerobically upon acetaldehyde was found to cause the peroxidation of linolenate. This was demonstrated by increased absorbance at 233 nm due to diene conjugation and by the detection of a lipid peroxide spot on the thin layer chromatograms. 2. Superoxide dismutase inhibited this lipid peroxid... | 171,266 | [
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0.2225197,
0.4808715,
0.4258632,
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Membranes of mammary gland. XI. Marker enzyme distribution profiles for membranous components from bovine mammary gland. | Enzyme distribution profiles of clarified bovine mammary homogenates separated by equilibrium centrifugation on linear sucrose gradients suggested that several of the commonly utilized marker enzymes for rat liver are also valid markers for mammary cellular components. These marker enzymes include: Succinate dehydrogen... | 171,290 | [
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Direct visualization of enveloped Epstein-Barr Herpesvirus in throat washing with leukocyte-transforming activity. | Mature enveloped virions belonging to the herpes class were found in a concentrated, partially purified specimen of throat washings from a patient undergoing immunosuppressive therapy for prevention of renal homograft rejection. This throat washing contained a high titer of biologically active Epstein-Barr virus and no... | 171,319 | [
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0.006726247,
0... |
'Phagocytosis' of sendai virus by model membranes. | Sendai viruses were attached to liposomes (vesicular model membranes) at 0 to 4 degrees C, and were then incubated at 37 degrees C. Liposomes made of phosphatidylcholine, cholesterol and gangliosides enveloped the viruses at 37 degrees C to give a picture that resembles the ingestion step of phagocytosis. Virus particl... | 171,337 | [
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0.09268756,
0.04132692,
0.1868109,
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Single fibre EMG findings in polyneuropathies of different aetiology. | Single fibre electromyography was carried out in patients with polyneuropathy due to uraemia, diabetes, and alcohol. In the two former groups the fibre density within the motor unit and the impulse transmission were mainly normal. In the latter group the fibre density was significantly increased as signs of reinnervati... | 171,347 | [
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0.0582763,
0.131017,
0.2725... |
Cerebral dynamic studies for early detection of recurrent anaplastic intracranial gliomas. | The authors compared radionuclide cerebral dynamic studies, brain scans, and clinical evaluation as indicators of recurrence of intracranial anaplastic gliomas and found cerebral dynamic studies more sensitive to tumor growth than static brain scans. The former may show changes prior to clinical evidence of tumor progr... | 171,351 | [
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0.2061589,
0.1504664,
0.215348,
0.03258646,
... |
The effects of stibophen on phosphofructokinases and aldolases of adult filariids. | Trivalent organic antimonials, such as stibophen, have been employed for the chemotherapy of schistosome and filariid infections. The effects of stibophen on adult Litomosoides carinii, Dipetalonema witei (= viteae), and Brugia pahangi were examined. In vitro, lactate accumulation was markedly inhibited by the antimoni... | 171,364 | [
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0.1703587,
0.1762175,
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0.1941627,
0.2252452,
0.1893667,
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... |
Metabolism of 8-(methylthio)cyclic 3',5'-adenosine monophosphate by rats and dogs after oral or intravenous dosing and in vitro by subcellular preparations of dog liver. | 8-(Methylthio-14C or -35S)cyclic 3',5'-adenosine monophosphate (I) was given intravenously to rats (5 mg/kg) and orally and intravenously to dogs (0.25, 2.5, or 50 mg/kg). Oral doses were absorbed well but slowly. Plasma half-lives in dogs were about 3 hr after oral or intravenous doses of 0.25 or 2.5 mg/kg and ranged ... | 171,373 | [
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0.1381091,
0.1934871,
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0.06037139,
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Disturbed wards--do we need them? | This is a study of forty-three female residents of a continued care state psychiatric facility who were transferred from a ward for highly disturbed persons to a ward based on a geographical unit system. One year after the transfer thirty-eight patients were improved, particularly in the area of behavior symptomatology... | 171,385 | [
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0.121315,
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0.2000039,
0.205347... |
Emergency discharges: their outcome. | This paper compares the short-term outcome of a group of seven patients released from an inpatient facility during an employee strike and a group of eight released at the optimal time according to the treating physicians. There was no difference in the outcome of the two groups, only two fifth of the entire cohort coul... | 171,392 | [
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0.02488285,
0.... |
Interrelationships between prostaglandins, cyclic AMP and steroids in ovulation. | Ovulation is a complex phenomenon, involving a series of biochemical events within the ovary, leading to the rupture of the follicle. This paper summarizes recent studies in our laboratory of some of these biochemical changes using the rabbit as an experimental model. It has been shown in our laboratory that isolated G... | 171,405 | [
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... |
A phlogistic function of PGE1 in calcium pyrophosphate dihydrate crystal-induced inflammation. | Promotion of calcium pyrophosphate dihydrate (CaPPD) crystal-induced inflammation by prostaglandin (PG) E1 has been demonstrated by two new techniques: (1) Rats deficient in essential fatty acids, biological precursors of PG, developed less footpad swelling than did normal rats following injections of unheated CaPPD. A... | 171,406 | [
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0.1210389,
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0.03056047,
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0.06999061,
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0.4369256,
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... |
Suppression of spontaneous in vitro transformation of autologous leukocytes by plasma from convalescent and postconvalescent infectious mononucleosis patients. | All 14 plasma samples from 13 convalescent and postconvalescent infectious mononucleosis (IM) patients suppressed the spontaneous in vitro transformation of autologous leukocytes. In contrast, only 2 of 8 plasma samples from patients with acute IM suppressed this transformation. All 7 patients whose blood was tested bo... | 171,425 | [
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0.2178895,
0.3158566,
-0.09688785,
0.04873354,
-... |
Histologic and electron microscopy observations on diethylnitrosamine-induced hepatomas in small aquarium fish (Oryzias latipes). | Liver neoplasms were induced in medakas (Oryzias latipes) by the addition of diethylnitrosamine (DENA) to their aquarium water at levels of 15-135 ppm for 8 weeks. After 13 weeks, 21 to 32 fish had developed hepatomas. Medakas are useful for further studies because they are highly susceptible to the carcinogenic effect... | 171,429 | [
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0.4037594,
0.01561083,
0.2592,
0.129518,
0.07758449,
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Transcription of simian virus 40. V. Regulattion of simian virus 40 gene expression. | RNA "exhaustion type" hybridization was used to measure the complementarity of nuclear and cytoplasmic viral RNA to the early (E) and late (L) simian virus 40 (SV40) DNA strands. This type of hybridization measures the amount of labeled RNA complementary to each of the two DNA strands, rather than the fraction of each ... | 171,448 | [
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0.648849,
0.1973669,
-0.052755,... |
The respiratory effects of chloromethyl methyl ether. | A prospective study of 125 chemical workers was carried out for ten years to investigate the incidence of lung cancer. Some of the men were exposed to chloromethyl methyl ether containing bis(chloromethyl) ether as an impurity. Bronchogenic carcinoma was markedly increased among them, with a strong dose-response relati... | 171,460 | [
-0.1599606,
0.1234911,
-0.1937249,
0.03376093,
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0.1817599,
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0.08344521,
0.09825297,
0.1455136,
0.005162759,
0.1408943,
-0.1301783,
-0.1493095,
-0.3605261,
0.1977059,
0.1835959,
0.2292667,
0.05430321,
0.2833287,
-0.1335028,
-0.... |
Adenoid cystic carcinoma (cylindroma) of the trachea masquerading as asthma. | A 40-year-old woman was admitted with upper airway obstruction; chest roentgenogram with tomograms, laryngoscopy, and bronchoscopy verified the presence of a subglottic tumor. Spirometry disclosed a maximum inspiratory flow rate more reduced than in the maximum expiratory flow rate, suggestive of upper airway obstructi... | 171,462 | [
0.02446065,
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0.02137818,
0.1246655,
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0.2699442,
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0.07172908,
... |
[Investigation of cyclic renal adenosinmonophosphate (yamp)-excretion in pseudohypoparathyreoidism (author's transl)]. | Report on a boy aged 6-1/2 years with pseudohypoparathyroidism. This is due to a genetic lack of PTH inducible adenocyclase. Even during vitamin D treatment renal camp formation by giving PTH extract cannot be stimulated, while in this situation. PTH shows a clearly phosphaturic effect. Whether this is due to a PTH pot... | 171,472 | [
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0.3179793,
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0.1416983,
0.2425641,
0.3341918,
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0.3423548,
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0.350562,
0.5186073,
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-0.1903436,
... |
[Plasma ACTH and Nelson tumors in bilaterally adrenalectomized Cushing patients]. | Bilaterally adrenalectomized patients (18) because of Cushing's syndrome were studied. 16 of these 18 persons exhibited fairly high plasma ACTH levels. Distinctly elevated plasma ACTH levels were observed in 6 of them. 5 patients of this group showed an enlarged sella and deep pigmentation of the skin. 4 of the latter ... | 171,474 | [
-0.02922623,
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0.07982059,
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0.2259091,
0.296468,
0.1147894,
0.2973056,
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0.005493469,
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0.02098558,
0.1250324,
0.06289586,
0.6105683,
0.1870995,
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-... |
Uremic neuropathy. III. Controlled study of restricted protein and fluid diet and infrequent hemodialysis versus conventional hemodialysis treatment. | The effect of a treatment schedule of restricted protein and fluid intake and of infrequent hemodialysis (schedule 1) as compared to a conventional hemodialysis treatment schedule (schedule 2) on the presence and severity of peripheral neuropathy has been studied in a small group of patients using a crossover design. U... | 171,524 | [
0.1442671,
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0.03948602,
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0.06544247,
0.06249417,
0.09505012,
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0.1389817,
0.1599931,
0.1455766,
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0.002621893,
-0.3910067,
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0.1203256,
0.2639262... |
Evidence for viral hepatitis other than type A or type B among persons in Costa Rica. | In studies of hepatitis in an endemic zone in Costa Rica, 103 patients were examined for antibodies against hepatitts A by the immune-adherence assay, for hepatitis B antigen and its antibody by radioimmunoassay and passive hemagglutination, respectively, and for antibodies against cytomegalovirus by complement fixatio... | 171,577 | [
-0.1622544,
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0.1558472,
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0.01656492,
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0.1902461,
0.3171479,
0.2045877,
0.1208153,
0.1838235,
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0.07191638,
0.1202169,
0.1300796,
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-0.2514904,
0... |
Synthesis of biological molecules on molecular sieves. | Catalytic properties of aluminosilicates may play a role in the synthesis of biological molecules from simple gaseous molecules commonly found in planetary atmospheres. Urea, amino acids and UV absorbing substances have been obtained by heating CO and NH3 with Linde molecular sieves saturated with Ca+2, NH4+ or Fe+3. T... | 171,609 | [
0.04827856,
0.105453,
0.1358403,
0.2183128,
0.1771253,
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0.03100485,
0.4490081,
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0.1630106,
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0.2172201,
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0.114629,
0.118263,
0.3238789,
0.2267075,
0.3066964,
-0.3633141,
0.19277... |
The synthesis of the internucleotide (phosphodiester) bond by a base-catalysed reaction. | Potassium tert-butoxide in hexamethyl phosphoramide and dimethyl formamide provides an excellent catalyst for the reaction of a 3'-hydroxyl or a 5'-hydroxyl group of a nucleoside with an appropriate nucleoside phosphorofluoridate to yield the dinucleoside phosphate. This paper describes the experiments leading to the d... | 171,626 | [
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0.08243857,
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0.05209055,
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0.2013363,
0.04697008,
0.2377073,
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0.3355891,
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0.3717019,
0.5175335,
-0.03066453,
-0.07049967... |
Interchain disulfide bonds in procollagen are located in a large nontriple-helical COOH-terminal domain. | Tadpole collagenase (EC 3.4.24.3) cleaved chick cranial bone procollagen into two triple-stranded fragments, PCA and PCB. Only PCB, with an estimated molecular weight of about 60,000 for each component chain after reduction, was found to contain interchain disulfide bonds. The analogous cleavage of collagen is known to... | 171,650 | [
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0.3094657,
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0.04720466,
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0.2945957,
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0.1262815,
0.08076701,
0.07150175,
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-0... |
Chemotactic signals induce cell differentiation in Dictyostelium discoideum. | Experiments carried out with the aid of cellophane membranes demonstrate that the morphogenetic block of certain nonaggregating, "aggregateless," mutants may be overcome by diffusible factors excreted by aggregating wild-type cells. The same differentiation process into aggregation-competent cell is observed if mutant ... | 171,655 | [
-0.3002355,
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0.2167251,
0.7641082,
0.1135444,
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... |
Periodate and concanavalin A induce blast transformation of rat lymphocytes by an indirect mechanism. | When rat lymph node cells, rendered incapable of division by treatment with mitomycin C, were then reacted with sodium metaperiodate (NaIO4), they stimulated the transformation of untreated, syngeneic lymph node cells in vitro. Unequivocal evidence that untreated lymph node cells responded in this situation was obtaine... | 171,668 | [
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0.09133186,
0.1882669,
0.3826274,
-0.08855512,
0.08953126,... |
Enhancement of immunity against murine syngeneic tumors by a fraction extracted from non-pathogenic mycobacteria. | The data reported here demonstrate that a preparation extracted from nonpathogenic mycobacteria such as Mycobacterium smegmatis and hereafter referred to as interphase material protected mice against Ehrlich ascitic carcinoma, L-1210 leukemia, and another syngeneic lymphoid leukemia. Furthermore, mice treated by this p... | 171,670 | [
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0.07875154,
0.1020272,
0.3584038,
0.3039131,
-0.3476057,
-0.0551451,
-0.1... |
Relationship between A-type and C-type particles in cells infected by Rous sarcoma virus. | Chicken cells infected with avian RNA tumor virus often contain small cytoplasmic A-type particles which commonly exist as clusters of 50--100 particles when viewed in thin sections. These particles were found more consistently in Rous sarcoma virus-infected than Rous-associated virus-infected cultures, but were genera... | 171,672 | [
-0.1518333,
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0.04108812,
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0.02270016,
0.3687872,
0.02748566,
0.07119995,
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0.4809909,
0.01560722,
0.4370512,
-0.04336605,
-0.292503... |
Adenosine, AMP, cyclic AMP, theophylline and the action and production of erythropoietin. | Erythropoiesis, as measured by the uptake of 59Fe into plethoric mice, is stimulated by adenosine, AMP, cyclic AMP, and dibutyryl cyclic AMP, but not by cytidine, its nucleotides or cyclic GMP. This stimulation is erythropoietin dependent, because it is prevented by anti-erythropoietin. Theophylline neither stimulates ... | 171,682 | [
-0.4721204,
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-0.02795199,
0.1571797,
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0.09304015,
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0.4384631,
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0.2929543,
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-0.220809,
0.01735464,
-0.5874885,
0.3441237,
0.2118213,
-0.07830859,
-0.220951... |
NMR in cancer. VII. Sodium-23 magnetic resonance of normal and cancerous tissues. | Sodium-23 magnetic resonance was performed on four types of cancers and six types of normal tissues of rats and mice. The spin-lattice relaxation time of the tumors was generally longer than that of the normal tissues, with the most marked difference occurring between rat liver (T1 = 6.5 msec) and Novikoff hepatoma (T1... | 171,689 | [
-0.1210731,
-0.188137,
-0.5599601,
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0.3094752,
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0.1570359,
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0.1093915,
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0.02325256,
0.242458,
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-0.02995517,
-0.1239671,
0.09577776,
0.3432375,
0.09722427,
-0.0845896... |
Effects of promethazine on nocturnal sleep in normal man. | The effects of a phenothiazine, on sleep in ten healthy volunteers were investigated in two double blind polygraphic studies. The first part consisted of a single dose study with promethazine 50, 100, and 200 mg, using pentobarbital 100 mg as a reference substance. In the second part, four subjects spent 20 consecutive... | 171,695 | [
-0.112971,
0.1312485,
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0.2219439,
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0.1565814,
0.1000511,
0.02862771,
0.3638627,
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-0.4877585,
0.4055206,
0.1666295,
0.0369572,
-0.06636263,
0.2404166,
0.1816718,
-0.2... |
The relationship between adenosine 3',5'-monophosphate levels and systolic transmembrane calcium flux. | The effects of inotropic agents on systolic transmembrane Ca++ flux were studied in isolated perfused guinea pig hearts depolarized with 22 mM K+. After depolarization, inotropic agents were added to the perfusion medium in an attempt to resotre excitability and contractions to the heart. Electrophysiological studies h... | 171,711 | [
-0.2694249,
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-0.3703672,
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0.03981411,
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0.1334513,
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0.1656019,
0.06446791,
0.2006586,
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0.1274227,
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-0.08733175,
0.3238821,
0.1335993,
0.005691827,
... |
[Some epidemiological indicators in bronchopulmonary cancer in Sector 5 of Bucharest (study of 525 cases)]. | The incidence was studied of broncho-pulmonary carcinoma, as well as of other epidemiological indicators in the 5th district of Bucharest over a period of 14 years (1960-1973) during which a total of 525 cases have occured. The incidence in this territory tends to become real from 1967 when the curve overtook the death... | 171,736 | [
-0.01523115,
-0.4647788,
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-0.1484725,
-0.3294235,
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0.004432441,
0.04097929,
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-0.01405968,
0.3930103,
0.3946722,
0.262564,
0.5092978,
0.4928994,
-0.1353359,
... |
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