paragraph_index
int64
sec
string
p_has_citation
int64
cites
string
citeids
list
pmid
int64
cited_id
string
sentences
string
all_sent_cites
list
sent_len
int64
sentence_batch_index
int64
sent_has_citation
float64
qc_fail
bool
cited_sentence
string
cites_in_sentence
list
cln_sentence
string
is_cap
bool
is_alpha
bool
ends_wp
bool
cit_qc
bool
lgtm
bool
__index_level_0__
int64
1
DISCUSSION
1
Rasala
[ "B114", "B65", "B117", "B1", "B2" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
Importantly, the sensitivity of channel formation to LPC that we see and its reversibility by OA or PE are consistent with a model where a hemifusion intermediate occurs between the outer and inner nuclear membranes during NPC assembly.
[ "Rasala ", "Hetzer and Wente, 2009", "Rotem ", "Anderson and Hetzer, 2007", "Antonin " ]
236
41,419
0
false
Importantly, the sensitivity of channel formation to LPC that we see and its reversibility by OA or PE are consistent with a model where a hemifusion intermediate occurs between the outer and inner nuclear membranes during NPC assembly.
[]
Importantly, the sensitivity of channel formation to LPC that we see and its reversibility by OA or PE are consistent with a model where a hemifusion intermediate occurs between the outer and inner nuclear membranes during NPC assembly.
true
true
true
true
true
7,142
1
DISCUSSION
1
Rasala
[ "B114", "B65", "B117", "B1", "B2" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
These data argue against an alternate model which proposes that the bulk of the nuclear pore is assembled on the surface of chromatin and acquires membranes by being surrounded by expanding ER tubules and cisternae (reviewed in Anderson and Hetzer, 2007; Antonin et al., 2008).
[ "Rasala ", "Hetzer and Wente, 2009", "Rotem ", "Anderson and Hetzer, 2007", "Antonin " ]
277
41,420
0
false
These data argue against an alternate model which proposes that the bulk of the nuclear pore is assembled on the surface of chromatin and acquires membranes by being surrounded by expanding ER tubules and cisternae.
[ "reviewed in Anderson and Hetzer, 2007; Antonin et al., 2008" ]
These data argue against an alternate model which proposes that the bulk of the nuclear pore is assembled on the surface of chromatin and acquires membranes by being surrounded by expanding ER tubules and cisternae.
true
true
true
true
true
7,142
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
The field is beginning to have an increasingly detailed view of the very early steps in postmitotic nuclear pore assembly.
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
122
41,421
0
false
The field is beginning to have an increasingly detailed view of the very early steps in postmitotic nuclear pore assembly.
[]
The field is beginning to have an increasingly detailed view of the very early steps in postmitotic nuclear pore assembly.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Specifically, the protein ELYS/MEL-28 acts by binding to chromatin via an AT-hook and at least one additional chromatin-binding domain.
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
135
41,422
0
false
Specifically, the protein ELYS/MEL-28 acts by binding to chromatin via an AT-hook and at least one additional chromatin-binding domain.
[]
Specifically, the protein ELYS/MEL-28 acts by binding to chromatin via an AT-hook and at least one additional chromatin-binding domain.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
ELYS either recruits the Nup107-160 complex to chromatin or is recruited in a joint complex with it to initiate pore assembly (Rasala et al., 2006; Franz et al., 2007; Gillespie et al., 2007; Rasala et al., 2008; Lau et al., 2009; Rotem et al., 2009).
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
251
41,423
0
false
ELYS either recruits the Nup107-160 complex to chromatin or is recruited in a joint complex with it to initiate pore assembly.
[ "Rasala et al., 2006; Franz et al., 2007; Gillespie et al., 2007; Rasala et al., 2008; Lau et al., 2009; Rotem et al., 2009" ]
ELYS either recruits the Nup107-160 complex to chromatin or is recruited in a joint complex with it to initiate pore assembly.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In the absence of these proteins, the NPC integral membrane proteins POM121 and Ndc1 are not recruited to the nuclear membrane (Rasala et al., 2008).
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
149
41,424
0
false
In the absence of these proteins, the NPC integral membrane proteins POM121 and Ndc1 are not recruited to the nuclear membrane.
[ "Rasala et al., 2008" ]
In the absence of these proteins, the NPC integral membrane proteins POM121 and Ndc1 are not recruited to the nuclear membrane.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
A recent study by (Doucet et al., 2010) used RNAi to partially knock down ELYS or POM121 in HeLa cells.
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
103
41,425
0
false
A recent study by used RNAi to partially knock down ELYS or POM121 in HeLa cells.
[ "Doucet et al., 2010" ]
A recent study by used RNAi to partially knock down ELYS or POM121 in HeLa cells.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
They concluded that these two proteins are differentially rate-limiting in interphase and mitotic pore assembly.
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
112
41,426
0
false
They concluded that these two proteins are differentially rate-limiting in interphase and mitotic pore assembly.
[]
They concluded that these two proteins are differentially rate-limiting in interphase and mitotic pore assembly.
true
true
true
true
true
7,143
2
DISCUSSION
1
Rasala
[ "B113", "B46", "B52", "B114", "B82", "B117", "B114", "B37", "B114" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Their results are consistent with our biochemical demonstration that POM121 can bind and pull down the Nup107/160 complex from Xenopus egg extracts (Rasala et al., 2008) as well as with the findings here where POM121 and the Nup107/160 complex colocalize on the inner nuclear membrane in a very early step in post-mitoti...
[ "Rasala ", "Franz ", "Gillespie ", "Rasala ", "Lau ", "Rotem ", "Rasala ", "Doucet ", "Rasala " ]
344
41,427
0
false
Their results are consistent with our biochemical demonstration that POM121 can bind and pull down the Nup107/160 complex from Xenopus egg extracts as well as with the findings here where POM121 and the Nup107/160 complex colocalize on the inner nuclear membrane in a very early step in post-mitotic nuclear pore assembl...
[ "Rasala et al., 2008" ]
Their results are consistent with our biochemical demonstration that POM121 can bind and pull down the Nup107/160 complex from Xenopus egg extracts as well as with the findings here where POM121 and the Nup107/160 complex colocalize on the inner nuclear membrane in a very early step in post-mitotic nuclear pore assembl...
true
true
true
true
true
7,143
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Inner/outer nuclear membrane fusion has previously proven to be a difficult step to detect or analyze.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
102
41,428
0
false
Inner/outer nuclear membrane fusion has previously proven to be a difficult step to detect or analyze.
[]
Inner/outer nuclear membrane fusion has previously proven to be a difficult step to detect or analyze.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Assembly of nuclear pores in existing nuclear membranes, such as yeast nuclei or S-phase vertebrate nuclei in vivo, must by definition involve interaction between the lumenal leaflets of the inner and outer nuclear membranes.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
225
41,429
0
false
Assembly of nuclear pores in existing nuclear membranes, such as yeast nuclei or S-phase vertebrate nuclei in vivo, must by definition involve interaction between the lumenal leaflets of the inner and outer nuclear membranes.
[]
Assembly of nuclear pores in existing nuclear membranes, such as yeast nuclei or S-phase vertebrate nuclei in vivo, must by definition involve interaction between the lumenal leaflets of the inner and outer nuclear membranes.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
There hemifusion would precede full fusion and thus would be predicted to be sensitive to arrest by the membrane fusion-inhibiting lipids LPC and OA.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
149
41,430
0
false
There hemifusion would precede full fusion and thus would be predicted to be sensitive to arrest by the membrane fusion-inhibiting lipids LPC and OA.
[]
There hemifusion would precede full fusion and thus would be predicted to be sensitive to arrest by the membrane fusion-inhibiting lipids LPC and OA.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In our in vitro nuclear assembly system, we could only add LPC or OA to the outer leaflet of the outer nuclear membrane of a cold intermediate, which is actually the leaflet facing away from the potential fusion event.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
218
41,431
0
false
In our in vitro nuclear assembly system, we could only add LPC or OA to the outer leaflet of the outer nuclear membrane of a cold intermediate, which is actually the leaflet facing away from the potential fusion event.
[]
In our in vitro nuclear assembly system, we could only add LPC or OA to the outer leaflet of the outer nuclear membrane of a cold intermediate, which is actually the leaflet facing away from the potential fusion event.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
However, multiple reports have shown that rapid interleaflet exchange of phospholipids occurs in ER-derived membranes via resident lipid flippases (Bishop and Bell, 1985; Herrmann et al., 1990; Buton et al., 1996; Marx et al., 2000; Papadopulos et al., 2007).
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
259
41,432
0
false
However, multiple reports have shown that rapid interleaflet exchange of phospholipids occurs in ER-derived membranes via resident lipid flippases.
[ "Bishop and Bell, 1985; Herrmann et al., 1990; Buton et al., 1996; Marx et al., 2000; Papadopulos et al., 2007" ]
However, multiple reports have shown that rapid interleaflet exchange of phospholipids occurs in ER-derived membranes via resident lipid flippases.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Thus, when LPC or OA was added to our cold intermediate, it should quickly equilibrate between the leaflets of the outer nuclear membrane.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
138
41,433
0
false
Thus, when LPC or OA was added to our cold intermediate, it should quickly equilibrate between the leaflets of the outer nuclear membrane.
[]
Thus, when LPC or OA was added to our cold intermediate, it should quickly equilibrate between the leaflets of the outer nuclear membrane.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
The presence of LPC in only one of two fusing bilayers has been shown to be sufficient to block fusion (Chernomordik et al., 1997;
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
130
41,434
0
false
The presence of LPC in only one of two fusing bilayers has been shown to be sufficient to block fusion (Chernomordik et al., 1997;
[]
The presence of LPC in only one of two fusing bilayers has been shown to be sufficient to block fusion (Chernomordik et al., 1997;
true
true
false
true
false
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Chernomordik et al., 1998).
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
27
41,435
0
false
Chernomordik et al., 1998).
[]
Chernomordik et al., 1998).
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Indeed, we observed that LPC prevented 3-kDa channel formation and FG nucleoporin recruitment in the cold intermediate.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
119
41,436
0
false
Indeed, we observed that LPC prevented 3-kDa channel formation and FG nucleoporin recruitment in the cold intermediate.
[]
Indeed, we observed that LPC prevented 3-kDa channel formation and FG nucleoporin recruitment in the cold intermediate.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
OA treatment also inhibited FG nucleoporin recruitment in this intermediate, but when added together, the two lipids counteracted one another and diffusion channel formation and FG recruitment proceeded, fulfilling the prediction above.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
236
41,437
0
false
OA treatment also inhibited FG nucleoporin recruitment in this intermediate, but when added together, the two lipids counteracted one another and diffusion channel formation and FG recruitment proceeded, fulfilling the prediction above.
[]
OA treatment also inhibited FG nucleoporin recruitment in this intermediate, but when added together, the two lipids counteracted one another and diffusion channel formation and FG recruitment proceeded, fulfilling the prediction above.
true
true
true
true
true
7,144
3
DISCUSSION
1
Bishop and Bell, 1985
[ "B7", "B63", "B14", "B95", "B108", "B19", "B16" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Thus, our data strongly argue that LPC and OA are acting to prevent inner/outer nuclear membrane fusion.
[ "Bishop and Bell, 1985", "Herrmann ", "Buton ", "Marx ", "Papadopulos ", "Chernomordik ", "Chernomordik " ]
104
41,438
0
false
Thus, our data strongly argue that LPC and OA are acting to prevent inner/outer nuclear membrane fusion.
[]
Thus, our data strongly argue that LPC and OA are acting to prevent inner/outer nuclear membrane fusion.
true
true
true
true
true
7,144
4
DISCUSSION
0
null
null
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA
Is there a way in which cold, LPC, or OA could be blocking NPC assembly other than by blocking fusion?
null
102
41,439
0
false
null
null
Is there a way in which cold, LPC, or OA could be blocking NPC assembly other than by blocking fusion?
true
true
true
true
true
7,145
4
DISCUSSION
0
null
null
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA
One speculative model might be that, normally, inner/outer nuclear membrane fusion occurs but results in formation of a tightly “closed” membranous tube that is so narrow as to be impermeable to 3-kDa dextrans.
null
210
41,440
0
false
null
null
One speculative model might be that, normally, inner/outer nuclear membrane fusion occurs but results in formation of a tightly “closed” membranous tube that is so narrow as to be impermeable to 3-kDa dextrans.
true
true
true
true
true
7,145
4
DISCUSSION
0
null
null
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA
Cold, LPC, or OA could then all possibly act by inhibiting the postfusion dilation of such a tube.
null
98
41,441
0
false
null
null
Cold, LPC, or OA could then all possibly act by inhibiting the postfusion dilation of such a tube.
true
true
true
true
true
7,145
4
DISCUSSION
0
null
null
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA
We think this unlikely, especially in light of their established role as fusion inhibitors in other studies.
null
108
41,442
0
false
null
null
We think this unlikely, especially in light of their established role as fusion inhibitors in other studies.
true
true
true
true
true
7,145
4
DISCUSSION
0
null
null
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA
However, if true, this alternative model would still be consistent with our conclusion that correct channel formation is blocked by the inhibitor LPC and reversed by addition of its counteracting lipid OA.
null
205
41,443
0
false
null
null
However, if true, this alternative model would still be consistent with our conclusion that correct channel formation is blocked by the inhibitor LPC and reversed by addition of its counteracting lipid OA.
true
true
true
true
true
7,145
5
DISCUSSION
1
Cohen
[ "B24", "B26", "B59", "B83", "B51", "B137", "B55", "B59", "B25", "B38", "B86", "B42", "B105", "B3", "B83", "B93", "B127" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
For inner/outer membrane fusion, a fusion machinery must of necessity bring the two membranes into close proximity.
[ "Cohen ", "Crisp and Burke, 2008", "Hallberg ", "Lau ", "Gerace ", "Wozniak ", "Greber ", "Hallberg ", "Cotter ", "Drummond and Wilson, 2002", "Liu ", "Eriksson ", "Olsson ", "Antonin ", "Lau ", "Mansfeld ", "Stavru " ]
115
41,444
0
false
For inner/outer membrane fusion, a fusion machinery must of necessity bring the two membranes into close proximity.
[]
For inner/outer membrane fusion, a fusion machinery must of necessity bring the two membranes into close proximity.
true
true
true
true
true
7,146
5
DISCUSSION
1
Cohen
[ "B24", "B26", "B59", "B83", "B51", "B137", "B55", "B59", "B25", "B38", "B86", "B42", "B105", "B3", "B83", "B93", "B127" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In structural terms, the 30–50 nm space (20–40 nm in yeast) must be bridged (Cohen et al., 2002; Crisp and Burke, 2008).
[ "Cohen ", "Crisp and Burke, 2008", "Hallberg ", "Lau ", "Gerace ", "Wozniak ", "Greber ", "Hallberg ", "Cotter ", "Drummond and Wilson, 2002", "Liu ", "Eriksson ", "Olsson ", "Antonin ", "Lau ", "Mansfeld ", "Stavru " ]
120
41,445
0
false
In structural terms, the 30–50 nm space (20–40 nm in yeast) must be bridged.
[ "Cohen et al., 2002; Crisp and Burke, 2008" ]
In structural terms, the 30–50 nm space must be bridged.
true
true
true
true
true
7,146
5
DISCUSSION
1
Cohen
[ "B24", "B26", "B59", "B83", "B51", "B137", "B55", "B59", "B25", "B38", "B86", "B42", "B105", "B3", "B83", "B93", "B127" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Of the three human integral membrane nucleoporins, POM121, Ndc1, and gp210, the first two have a very small lumenal presence (33–39 aa and 21–30 aa) (Hallberg et al., 1993; Lau et al., 2006).
[ "Cohen ", "Crisp and Burke, 2008", "Hallberg ", "Lau ", "Gerace ", "Wozniak ", "Greber ", "Hallberg ", "Cotter ", "Drummond and Wilson, 2002", "Liu ", "Eriksson ", "Olsson ", "Antonin ", "Lau ", "Mansfeld ", "Stavru " ]
191
41,446
0
false
Of the three human integral membrane nucleoporins, POM121, Ndc1, and gp210, the first two have a very small lumenal presence (33–39 aa and 21–30 aa).
[ "Hallberg et al., 1993; Lau et al., 2006" ]
Of the three human integral membrane nucleoporins, POM121, Ndc1, and gp210, the first two have a very small lumenal presence.
true
true
true
true
true
7,146
5
DISCUSSION
1
Cohen
[ "B24", "B26", "B59", "B83", "B51", "B137", "B55", "B59", "B25", "B38", "B86", "B42", "B105", "B3", "B83", "B93", "B127" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Gp210 has a large lumenal domain (1782 aa) (Gerace et al., 1982), but has been found to be dispensable in some studies, both by RNAi depletion and in certain cells that naturally lack gp210 (Wozniak et al., 1989; Greber et al., 1990; Hallberg et al., 1993; Cotter et al., 1998; Drummond and Wilson, 2002; Liu et al., 200...
[ "Cohen ", "Crisp and Burke, 2008", "Hallberg ", "Lau ", "Gerace ", "Wozniak ", "Greber ", "Hallberg ", "Cotter ", "Drummond and Wilson, 2002", "Liu ", "Eriksson ", "Olsson ", "Antonin ", "Lau ", "Mansfeld ", "Stavru " ]
452
41,447
0
false
Gp210 has a large lumenal domain (1782 aa), but has been found to be dispensable in some studies, both by RNAi depletion and in certain cells that naturally lack gp210.
[ "Gerace et al., 1982", "Wozniak et al., 1989; Greber et al., 1990; Hallberg et al., 1993; Cotter et al., 1998; Drummond and Wilson, 2002; Liu et al., 2003; Eriksson et al., 2004; Olsson et al., 2004; Antonin et al., 2005; Lau et al., 2006; Mansfeld et al., 2006; Stavru et al., 2006a" ]
Gp210 has a large lumenal domain, but has been found to be dispensable in some studies, both by RNAi depletion and in certain cells that naturally lack gp210.
true
true
true
true
true
7,146
5
DISCUSSION
1
Cohen
[ "B24", "B26", "B59", "B83", "B51", "B137", "B55", "B59", "B25", "B38", "B86", "B42", "B105", "B3", "B83", "B93", "B127" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Thus, neither Pom121, Ndc1, nor gp210 appears on its own to fill the requirements for bridging the lumenal gap needed for inner/outer membrane fusion.
[ "Cohen ", "Crisp and Burke, 2008", "Hallberg ", "Lau ", "Gerace ", "Wozniak ", "Greber ", "Hallberg ", "Cotter ", "Drummond and Wilson, 2002", "Liu ", "Eriksson ", "Olsson ", "Antonin ", "Lau ", "Mansfeld ", "Stavru " ]
150
41,448
0
false
Thus, neither Pom121, Ndc1, nor gp210 appears on its own to fill the requirements for bridging the lumenal gap needed for inner/outer membrane fusion.
[]
Thus, neither Pom121, Ndc1, nor gp210 appears on its own to fill the requirements for bridging the lumenal gap needed for inner/outer membrane fusion.
true
true
true
true
true
7,146
6
DISCUSSION
1
Fridkin
[ "B47", "B69", "B132", "B130", "B26", "B13", "B87" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
The inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins together form long proteinaceous tethers across the intermembrane space and theoretically could be the bridge (Fridkin et al., 2004; Holaska and Wilson, 2006; Tzur et al., 2006; Stewart et al., 2007; Crisp and Burke, 2008; Burke and Roux, ...
[ "Fridkin ", "Holaska and Wilson, 2006", "Tzur ", "Stewart ", "Crisp and Burke, 2008", "Burke and Roux, 2009", "Liu " ]
326
41,449
0
false
The inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins together form long proteinaceous tethers across the intermembrane space and theoretically could be the bridge.
[ "Fridkin et al., 2004; Holaska and Wilson, 2006; Tzur et al., 2006; Stewart et al., 2007; Crisp and Burke, 2008; Burke and Roux, 2009" ]
The inner nuclear membrane SUN proteins and outer nuclear membrane KASH proteins together form long proteinaceous tethers across the intermembrane space and theoretically could be the bridge.
true
true
true
true
true
7,147
6
DISCUSSION
1
Fridkin
[ "B47", "B69", "B132", "B130", "B26", "B13", "B87" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
No involvement in nuclear membrane fusion events, however, has been observed for these proteins, but Sun1 at least has been found to be associated with the nuclear pore in mammalian cells (Liu et al., 2007).
[ "Fridkin ", "Holaska and Wilson, 2006", "Tzur ", "Stewart ", "Crisp and Burke, 2008", "Burke and Roux, 2009", "Liu " ]
207
41,450
0
false
No involvement in nuclear membrane fusion events, however, has been observed for these proteins, but Sun1 at least has been found to be associated with the nuclear pore in mammalian cells.
[ "Liu et al., 2007" ]
No involvement in nuclear membrane fusion events, however, has been observed for these proteins, but Sun1 at least has been found to be associated with the nuclear pore in mammalian cells.
true
true
true
true
true
7,147
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
The reticulon and Yop1/DP1 protein families, involved in ER membrane-bending, play a role in nuclear pore assembly in yeast (Voeltz et al., 2006; Hu et al., 2008; Shibata et al., 2008; Dawson et al., 2009).
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
206
41,451
0
false
The reticulon and Yop1/DP1 protein families, involved in ER membrane-bending, play a role in nuclear pore assembly in yeast.
[ "Voeltz et al., 2006; Hu et al., 2008; Shibata et al., 2008; Dawson et al., 2009" ]
The reticulon and Yop1/DP1 protein families, involved in ER membrane-bending, play a role in nuclear pore assembly in yeast.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In addition, in the Xenopus nuclear assembly system, antibodies to Reticulon-4a inhibit new pore assembly (Dawson et al., 2009).
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
128
41,452
0
false
In addition, in the Xenopus nuclear assembly system, antibodies to Reticulon-4a inhibit new pore assembly.
[ "Dawson et al., 2009" ]
In addition, in the Xenopus nuclear assembly system, antibodies to Reticulon-4a inhibit new pore assembly.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Other proteins of the yeast ER and/or nuclear membrane have also been observed to affect nuclear pores at some level, including Apq12, Brr6, Snl1, and Acc1 (Hodge et al.,; Schneiter et al., 1996; de Bruyn Kops and Guthrie, 2001; Sondermann et al., 2002; Scarcelli et al., 2007).
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
278
41,453
0
false
Other proteins of the yeast ER and/or nuclear membrane have also been observed to affect nuclear pores at some level, including Apq12, Brr6, Snl1, and Acc1.
[ "Hodge et al.,; Schneiter et al., 1996; de Bruyn Kops and Guthrie, 2001; Sondermann et al., 2002; Scarcelli et al., 2007" ]
Other proteins of the yeast ER and/or nuclear membrane have also been observed to affect nuclear pores at some level, including Apq12, Brr6, Snl1, and Acc1.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
Interestingly, Apq12 null yeast are cold sensitive for growth.
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
62
41,454
0
false
Interestingly, Apq12 null yeast are cold sensitive for growth.
[]
Interestingly, Apq12 null yeast are cold sensitive for growth.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
They and brr6-1 mutant cells appear to have electron dense NPC-like structures that are associated only with the inner nuclear membrane and not with the outer membrane (Hodge et al., 2010; Scarcelli et al., 2007).
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
213
41,455
0
false
They and brr6-1 mutant cells appear to have electron dense NPC-like structures that are associated only with the inner nuclear membrane and not with the outer membrane.
[ "Hodge et al., 2010; Scarcelli et al., 2007" ]
They and brr6-1 mutant cells appear to have electron dense NPC-like structures that are associated only with the inner nuclear membrane and not with the outer membrane.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
However, it is not yet known whether any of the above proteins have a connection to inner/outer membrane fusion.
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
112
41,456
0
false
However, it is not yet known whether any of the above proteins have a connection to inner/outer membrane fusion.
[]
However, it is not yet known whether any of the above proteins have a connection to inner/outer membrane fusion.
true
true
true
true
true
7,148
7
DISCUSSION
1
Voeltz
[ "B134", "B70", "B124", "B30", "B30", "B121", "B31", "B126", "B120", "B68", "B120" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA|NA|NA
In summary, the fusion protein(s) for nuclear pore assembly await discovery.
[ "Voeltz ", "Hu ", "Shibata ", "Dawson ", "Dawson ", "Schneiter ", "de Bruyn Kops and Guthrie, 2001", "Sondermann ", "Scarcelli ", "Hodge ", "Scarcelli " ]
76
41,457
0
false
In summary, the fusion protein(s) for nuclear pore assembly await discovery.
[]
In summary, the fusion protein(s) for nuclear pore assembly await discovery.
true
true
true
true
true
7,148
8
DISCUSSION
1
Siniossoglou
[ "B125", "B90", "B5", "B36", "B33", "B12", "B12", "B35", "B106" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
The next question, beyond the issue of what causes fusion, is how the fusion pore becomes limited in its expansion to ∼900 Å, the width of the actual membrane passage that connects the nuclear interior to the cytoplasm.
[ "Siniossoglou ", "Lutzmann ", "Beck ", "Devos ", "Debler ", "Brohawn and Schwartz, 2009", "Brohawn and Schwartz, 2009", "DeGrasse ", "Onischenko " ]
219
41,458
0
false
The next question, beyond the issue of what causes fusion, is how the fusion pore becomes limited in its expansion to ∼900 Å, the width of the actual membrane passage that connects the nuclear interior to the cytoplasm.
[]
The next question, beyond the issue of what causes fusion, is how the fusion pore becomes limited in its expansion to ∼900 Å, the width of the actual membrane passage that connects the nuclear interior to the cytoplasm.
true
true
true
true
true
7,149
8
DISCUSSION
1
Siniossoglou
[ "B125", "B90", "B5", "B36", "B33", "B12", "B12", "B35", "B106" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
The Nup107-160 complex has a Y-shape (∼230 Å × ∼420 Å) and has been hypothesized to form a multimeric lattice that could line and stabilize this passageway (Siniossoglou et al., 2000; Lutzmann et al., 2002; Beck et al., 2004; Devos et al., 2004; Debler et al., 2008; Brohawn and Schwartz, 2009).
[ "Siniossoglou ", "Lutzmann ", "Beck ", "Devos ", "Debler ", "Brohawn and Schwartz, 2009", "Brohawn and Schwartz, 2009", "DeGrasse ", "Onischenko " ]
295
41,459
0
false
The Nup107-160 complex has a Y-shape (∼230 Å × ∼420 Å) and has been hypothesized to form a multimeric lattice that could line and stabilize this passageway.
[ "Siniossoglou et al., 2000; Lutzmann et al., 2002; Beck et al., 2004; Devos et al., 2004; Debler et al., 2008; Brohawn and Schwartz, 2009" ]
The Nup107-160 complex has a Y-shape and has been hypothesized to form a multimeric lattice that could line and stabilize this passageway.
true
true
true
true
true
7,149
8
DISCUSSION
1
Siniossoglou
[ "B125", "B90", "B5", "B36", "B33", "B12", "B12", "B35", "B106" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
In strong support of such a lattice model, crystallographic analysis of the Nup96/sec13 and Nup85/seh1 proteins, components of the Nup107-160 complex, are closely related in 3D structures to the sec31/sec13 complex that forms the lattice-like coat of COP II transport vesicles (Brohawn and Schwartz, 2009; DeGrasse et al...
[ "Siniossoglou ", "Lutzmann ", "Beck ", "Devos ", "Debler ", "Brohawn and Schwartz, 2009", "Brohawn and Schwartz, 2009", "DeGrasse ", "Onischenko " ]
329
41,460
0
false
In strong support of such a lattice model, crystallographic analysis of the Nup96/sec13 and Nup85/seh1 proteins, components of the Nup107-160 complex, are closely related in 3D structures to the sec31/sec13 complex that forms the lattice-like coat of COP II transport vesicles.
[ "Brohawn and Schwartz, 2009; DeGrasse et al., 2009" ]
In strong support of such a lattice model, crystallographic analysis of the Nup96/sec13 and Nup85/seh1 proteins, components of the Nup107-160 complex, are closely related in 3D structures to the sec31/sec13 complex that forms the lattice-like coat of COP II transport vesicles.
true
true
true
true
true
7,149
8
DISCUSSION
1
Siniossoglou
[ "B125", "B90", "B5", "B36", "B33", "B12", "B12", "B35", "B106" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
We would suggest that a ring of Nup107-160 complexes that is multimerized on the surface of the inner nuclear membrane and attached to the membrane by POM121 could act as a preset ring that limits fusion pore expansion.
[ "Siniossoglou ", "Lutzmann ", "Beck ", "Devos ", "Debler ", "Brohawn and Schwartz, 2009", "Brohawn and Schwartz, 2009", "DeGrasse ", "Onischenko " ]
219
41,461
0
false
We would suggest that a ring of Nup107-160 complexes that is multimerized on the surface of the inner nuclear membrane and attached to the membrane by POM121 could act as a preset ring that limits fusion pore expansion.
[]
We would suggest that a ring of Nup107-160 complexes that is multimerized on the surface of the inner nuclear membrane and attached to the membrane by POM121 could act as a preset ring that limits fusion pore expansion.
true
true
true
true
true
7,149
8
DISCUSSION
1
Siniossoglou
[ "B125", "B90", "B5", "B36", "B33", "B12", "B12", "B35", "B106" ]
20,926,687
NA|NA|NA|NA|NA|NA|NA|NA|NA
This Nup107-160 complex could also require other proteins for stabilization of the membrane channel, as in yeast the depletion of a subset of membrane nucleoporins (Ndc1, Pom152, and Pom34), together with Nup59 or Nup53 depletion, results in nuclear pores that are noticeably dilated (Onischenko et al., 2009).
[ "Siniossoglou ", "Lutzmann ", "Beck ", "Devos ", "Debler ", "Brohawn and Schwartz, 2009", "Brohawn and Schwartz, 2009", "DeGrasse ", "Onischenko " ]
310
41,462
0
false
This Nup107-160 complex could also require other proteins for stabilization of the membrane channel, as in yeast the depletion of a subset of membrane nucleoporins (Ndc1, Pom152, and Pom34), together with Nup59 or Nup53 depletion, results in nuclear pores that are noticeably dilated.
[ "Onischenko et al., 2009" ]
This Nup107-160 complex could also require other proteins for stabilization of the membrane channel, as in yeast the depletion of a subset of membrane nucleoporins, together with Nup59 or Nup53 depletion, results in nuclear pores that are noticeably dilated.
true
true
true
true
true
7,149
9
DISCUSSION
1
Huebner and Gutzeit, 1986
[ "B71", "B23", "B98" ]
20,926,687
NA|NA|NA
Large membrane-lined channels connecting two entities are rare in evolution.
[ "Huebner and Gutzeit, 1986", "Cilia and Jackson, 2004", "Mische " ]
76
41,463
0
false
Large membrane-lined channels connecting two entities are rare in evolution.
[]
Large membrane-lined channels connecting two entities are rare in evolution.
true
true
true
true
true
7,150
9
DISCUSSION
1
Huebner and Gutzeit, 1986
[ "B71", "B23", "B98" ]
20,926,687
NA|NA|NA
Among the few known examples are the plasmodesmata channels that connect certain plant cells to one another, and the ring channels that connect Drosophila nurse cells to the oocyte that they supply (Huebner and Gutzeit, 1986; Cilia and Jackson, 2004; Mische et al., 2007).
[ "Huebner and Gutzeit, 1986", "Cilia and Jackson, 2004", "Mische " ]
272
41,464
0
false
Among the few known examples are the plasmodesmata channels that connect certain plant cells to one another, and the ring channels that connect Drosophila nurse cells to the oocyte that they supply.
[ "Huebner and Gutzeit, 1986; Cilia and Jackson, 2004; Mische et al., 2007" ]
Among the few known examples are the plasmodesmata channels that connect certain plant cells to one another, and the ring channels that connect Drosophila nurse cells to the oocyte that they supply.
true
true
true
true
true
7,150
9
DISCUSSION
1
Huebner and Gutzeit, 1986
[ "B71", "B23", "B98" ]
20,926,687
NA|NA|NA
These two examples, however, are thought to result from incomplete cytokinesis, rather than by fusion pore expansion and stabilization as in the case of the NPC.
[ "Huebner and Gutzeit, 1986", "Cilia and Jackson, 2004", "Mische " ]
161
41,465
0
false
These two examples, however, are thought to result from incomplete cytokinesis, rather than by fusion pore expansion and stabilization as in the case of the NPC.
[]
These two examples, however, are thought to result from incomplete cytokinesis, rather than by fusion pore expansion and stabilization as in the case of the NPC.
true
true
true
true
true
7,150
9
DISCUSSION
1
Huebner and Gutzeit, 1986
[ "B71", "B23", "B98" ]
20,926,687
NA|NA|NA
The stabilized inner/outer membrane fusion product that is the nuclear pore complex may thus be a unique occurence in evolution.
[ "Huebner and Gutzeit, 1986", "Cilia and Jackson, 2004", "Mische " ]
128
41,466
0
false
The stabilized inner/outer membrane fusion product that is the nuclear pore complex may thus be a unique occurence in evolution.
[]
The stabilized inner/outer membrane fusion product that is the nuclear pore complex may thus be a unique occurence in evolution.
true
true
true
true
true
7,150
10
DISCUSSION
0
null
null
20,926,687
null
In conclusion, this study maps diffusion channel formation downstream from POM121 and Nup107-160 complex recruitment, and upstream or coincident with FG nucleoporin recruitment.
null
177
41,467
0
false
null
null
In conclusion, this study maps diffusion channel formation downstream from POM121 and Nup107-160 complex recruitment, and upstream or coincident with FG nucleoporin recruitment.
true
true
true
true
true
7,151
10
DISCUSSION
0
null
null
20,926,687
null
These results thus order the important fusion step in NPC assembly with respect to other key steps (Figure 8): (i) AT-rich chromatin recruitment of ELYS and the Nup107-160 complex, (ii) colocalization of the Nup107-160 complex with POM121/Ndc1 at the inner nuclear membrane, followed by (iii) inner/outer membrane fusion...
null
381
41,468
0
false
null
null
These results thus order the important fusion step in NPC assembly with respect to other key steps (Figure 8): (i) AT-rich chromatin recruitment of ELYS and the Nup107-160 complex, (ii) colocalization of the Nup107-160 complex with POM121/Ndc1 at the inner nuclear membrane, followed by (iii) inner/outer membrane fusion...
true
true
true
true
true
7,151
10
DISCUSSION
0
null
null
20,926,687
null
The frontier of nuclear pore assembly now requires identification of the fusogen and determination of the order of addition of the myriad subunits that go on to build the pore complex within this evolutionarily unique membranous channel.
null
237
41,469
0
false
null
null
The frontier of nuclear pore assembly now requires identification of the fusogen and determination of the order of addition of the myriad subunits that go on to build the pore complex within this evolutionarily unique membranous channel.
true
true
true
true
true
7,151
11
DISCUSSION
0
null
null
20,926,687
null
A schematic model for inner/outer nuclear membrane fusion in the early steps of vertebrate NPC assembly.
null
104
41,470
0
false
null
null
A schematic model for inner/outer nuclear membrane fusion in the early steps of vertebrate NPC assembly.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
In previous studies, ELYS was shown to mediate the binding of the Nup107-160 complex to sites of AT-rich chromatin (blue) in early nuclear assembly.
null
148
41,471
0
false
null
null
In previous studies, ELYS was shown to mediate the binding of the Nup107-160 complex to sites of AT-rich chromatin (blue) in early nuclear assembly.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
Simultaneous with this ER-derived membranes are recruited to the rim of developing nuclei.
null
90
41,472
0
false
null
null
Simultaneous with this ER-derived membranes are recruited to the rim of developing nuclei.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
The data presented here argues that next: (1) the Nup107-160 complex (detected by anti-Nup133 antibody) interacts with POM121 at the inner nuclear membrane in an early nuclear intermediate (30 min, 14°C).
null
204
41,473
0
false
null
null
The data presented here argues that next: (1) the Nup107-160 complex (detected by anti-Nup133 antibody) interacts with POM121 at the inner nuclear membrane in an early nuclear intermediate (30 min, 14°C).
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
Bending of the membranes toward one another would theoretically occur at or near this time.
null
91
41,474
0
false
null
null
Bending of the membranes toward one another would theoretically occur at or near this time.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
(2) This is followed by fusion of the inner and outer nuclear membranes to form a diffusion channel.
null
100
41,475
0
false
null
null
(2) This is followed by fusion of the inner and outer nuclear membranes to form a diffusion channel.
false
false
true
true
false
7,152
11
DISCUSSION
0
null
null
20,926,687
null
(3) This fusion is simultaneous with or precedes recruitment of the bulk of FG nucleoporins (gold) and assembly of the mature nuclear pore, which involves expansion of the initial channel and its stabilization.
null
210
41,476
0
false
null
null
(3) This fusion is simultaneous with or precedes recruitment of the bulk of FG nucleoporins (gold) and assembly of the mature nuclear pore, which involves expansion of the initial channel and its stabilization.
false
false
true
true
false
7,152
11
DISCUSSION
0
null
null
20,926,687
null
In pioneering membrane-membrane fusion studies, progression to hemifusion was seen to be typically inhibited by cone-shaped lipids, such as LPC; here LPC was found to indeed block channel formation and FG Nup assembly in nuclear intermediates.
null
243
41,477
0
false
null
null
In pioneering membrane-membrane fusion studies, progression to hemifusion was seen to be typically inhibited by cone-shaped lipids, such as LPC; here LPC was found to indeed block channel formation and FG Nup assembly in nuclear intermediates.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
Progression beyond hemifusion to full fusion has been observed to be inhibited by inverted cone-shaped lipids, such as OA; here we found that OA also blocks mature nuclear pore assembly.
null
186
41,478
0
false
null
null
Progression beyond hemifusion to full fusion has been observed to be inhibited by inverted cone-shaped lipids, such as OA; here we found that OA also blocks mature nuclear pore assembly.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
When added together LPC and OA are known to geometrically neutralize one another, and we observed that LPC+OA allow channel formation, i.e., inner/outer membrane fusion.
null
169
41,479
0
false
null
null
When added together LPC and OA are known to geometrically neutralize one another, and we observed that LPC+OA allow channel formation, i.e., inner/outer membrane fusion.
true
true
true
true
true
7,152
11
DISCUSSION
0
null
null
20,926,687
null
(It is important to note that we do not believe that a completely sealed nuclear envelope is required before nuclear pores can form, but possession of such a sealed intermediate allowed us to distinguish and analyze the channel formation step of nuclear pore assembly.)
null
269
41,480
0
false
null
null
(It is important to note that we do not believe that a completely sealed nuclear envelope is required before nuclear pores can form, but possession of such a sealed intermediate allowed us to distinguish and analyze the channel formation step of nuclear pore assembly.)
false
false
false
true
false
7,152
0
DISCUSSION
1
20
[ "bib20" ]
19,047,440
pmid-6978196
In this study, we have used systems biology and polychromatic flow cytometry to characterize the early immunological processes that are initiated by one of the most potent vaccines ever generated, the YF vaccine YF17D, and which culminate into persistent immunological memory and long-term protection against a challenge...
[ "20" ]
336
41,481
1
false
In this study, we have used systems biology and polychromatic flow cytometry to characterize the early immunological processes that are initiated by one of the most potent vaccines ever generated, the YF vaccine YF17D, and which culminate into persistent immunological memory and long-term protection against a challenge...
[ "20" ]
In this study, we have used systems biology and polychromatic flow cytometry to characterize the early immunological processes that are initiated by one of the most potent vaccines ever generated, the YF vaccine YF17D, and which culminate into persistent immunological memory and long-term protection against a challenge...
true
true
true
true
true
7,153
0
DISCUSSION
1
20
[ "bib20" ]
19,047,440
pmid-6978196
The vaccine induced a significant modulation of 594 genes in whole blood cells, with the highest number of genes being induced at day 7 after vaccination.
[ "20" ]
154
41,482
0
false
The vaccine induced a significant modulation of 594 genes in whole blood cells, with the highest number of genes being induced at day 7 after vaccination.
[]
The vaccine induced a significant modulation of 594 genes in whole blood cells, with the highest number of genes being induced at day 7 after vaccination.
true
true
true
true
true
7,153
0
DISCUSSION
1
20
[ "bib20" ]
19,047,440
pmid-6978196
ICA and gene set enrichment allowed us to identify several nodes of transcriptional regulation that became induced within the first week after immunization.
[ "20" ]
156
41,483
0
false
ICA and gene set enrichment allowed us to identify several nodes of transcriptional regulation that became induced within the first week after immunization.
[]
ICA and gene set enrichment allowed us to identify several nodes of transcriptional regulation that became induced within the first week after immunization.
true
true
true
true
true
7,153
0
DISCUSSION
1
20
[ "bib20" ]
19,047,440
pmid-6978196
This early response was highly integrated, as several of the downstream target genes were coordinately regulated by these transcription factors.
[ "20" ]
144
41,484
0
false
This early response was highly integrated, as several of the downstream target genes were coordinately regulated by these transcription factors.
[]
This early response was highly integrated, as several of the downstream target genes were coordinately regulated by these transcription factors.
true
true
true
true
true
7,153
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
Of the identified nodes, IRF7 was prominently involved in this masterswitch regulation.
[ "21", "22", "23", "22", "24" ]
87
41,485
0
false
Of the identified nodes, IRF7 was prominently involved in this masterswitch regulation.
[]
Of the identified nodes, IRF7 was prominently involved in this masterswitch regulation.
true
true
true
true
true
7,154
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
The induction of IRF7 has been shown to mediate innate and adaptive immunity against many viruses, including encephalomyocarditis virus, vesicular stomatitis virus, influenza virus, and Sindbis virus (21, 22), confirming the significance of this gene in mediating protective immunity and in inducing strong innate and ad...
[ "21", "22", "23", "22", "24" ]
344
41,486
0
false
The induction of IRF7 has been shown to mediate innate and adaptive immunity against many viruses, including encephalomyocarditis virus, vesicular stomatitis virus, influenza virus, and Sindbis virus, confirming the significance of this gene in mediating protective immunity and in inducing strong innate and adaptive im...
[ "21, 22" ]
The induction of IRF7 has been shown to mediate innate and adaptive immunity against many viruses, including encephalomyocarditis virus, vesicular stomatitis virus, influenza virus, and Sindbis virus, confirming the significance of this gene in mediating protective immunity and in inducing strong innate and adaptive im...
true
true
true
true
true
7,154
1
DISCUSSION
1
23
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
It was also recently reported that the YF17D virus activates DCs by triggering their TLR2, 7, 8, and 9 (23).
[ "21", "22", "23", "22", "24" ]
108
41,487
1
false
It was also recently reported that the YF17D virus activates DCs by triggering their TLR2, 7, 8, and 9.
[ "23" ]
It was also recently reported that the YF17D virus activates DCs by triggering their TLR2, 7, 8, and 9.
true
true
true
true
true
7,154
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
Our data reveal that TLR7 and its downstream adaptor molecule Myd88 are both up-regulated upon vaccination with YF17D.
[ "21", "22", "23", "22", "24" ]
118
41,488
0
false
Our data reveal that TLR7 and its downstream adaptor molecule Myd88 are both up-regulated upon vaccination with YF17D.
[]
Our data reveal that TLR7 and its downstream adaptor molecule Myd88 are both up-regulated upon vaccination with YF17D.
true
true
true
true
true
7,154
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
TLR7 is a molecular sensor for single-stranded RNA, such as the nucleic acid found in YF17D and other flaviviruses, and triggering of TLR7 causes the transduction of a signal, via Myd88, to up-regulate expression of inflammatory cytokines such as IL-6, IL-12, and TNF (via NF-κB) and type I IFNs (via IRF7) (22, 24).
[ "21", "22", "23", "22", "24" ]
316
41,489
0
false
TLR7 is a molecular sensor for single-stranded RNA, such as the nucleic acid found in YF17D and other flaviviruses, and triggering of TLR7 causes the transduction of a signal, via Myd88, to up-regulate expression of inflammatory cytokines such as IL-6, IL-12, and TNF (via NF-κB) and type I IFNs (via IRF7).
[ "22, 24" ]
TLR7 is a molecular sensor for single-stranded RNA, such as the nucleic acid found in YF17D and other flaviviruses, and triggering of TLR7 causes the transduction of a signal, via Myd88, to up-regulate expression of inflammatory cytokines such as IL-6, IL-12, and TNF (via NF-κB) and type I IFNs (via IRF7).
true
true
true
true
true
7,154
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
Type I IFNs in turn enhance the expression of proteins with direct antiviral activity, such as ISG20 and OAS1, 2, and 3, which lead to viral RNA degradation, and MX1, MX2, ADAR, and EIF2AK2, which inhibit viral replication.
[ "21", "22", "23", "22", "24" ]
223
41,490
0
false
Type I IFNs in turn enhance the expression of proteins with direct antiviral activity, such as ISG20 and OAS1, 2, and 3, which lead to viral RNA degradation, and MX1, MX2, ADAR, and EIF2AK2, which inhibit viral replication.
[]
Type I IFNs in turn enhance the expression of proteins with direct antiviral activity, such as ISG20 and OAS1, 2, and 3, which lead to viral RNA degradation, and MX1, MX2, ADAR, and EIF2AK2, which inhibit viral replication.
true
true
true
true
true
7,154
1
DISCUSSION
1
21
[ "bib21", "bib22", "bib23", "bib22", "bib24" ]
19,047,440
pmid-18272964|pmid-15800576|pmid-16461338|pmid-15800576|pmid-16410796
We are showing that all these genes are up-regulated upon YF17D vaccination.
[ "21", "22", "23", "22", "24" ]
76
41,491
0
false
We are showing that all these genes are up-regulated upon YF17D vaccination.
[]
We are showing that all these genes are up-regulated upon YF17D vaccination.
true
true
true
true
true
7,154
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
Another prominent node of transcription regulation induced by YF17D vaccination is the ETS2 transcription factor.
[ "25", "26", "27", "28", "25", "29" ]
113
41,492
0
false
Another prominent node of transcription regulation induced by YF17D vaccination is the ETS2 transcription factor.
[]
Another prominent node of transcription regulation induced by YF17D vaccination is the ETS2 transcription factor.
true
true
true
true
true
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
ETS2 is involved in the differentiation and maturation of several immune cell types (25).
[ "25", "26", "27", "28", "25", "29" ]
89
41,493
1
false
ETS2 is involved in the differentiation and maturation of several immune cell types.
[ "25" ]
ETS2 is involved in the differentiation and maturation of several immune cell types.
true
true
true
true
true
7,155
2
DISCUSSION
1
26
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
Its expression is up-regulated in activated and proliferating T cells (26) and ETS2 is involved in IL-12 p40 (Th1) and IL-5 (Th2) gene expression (27, 28).
[ "25", "26", "27", "28", "25", "29" ]
155
41,494
1
false
Its expression is up-regulated in activated and proliferating T cells and ETS2 is involved in IL-12 p40 (Th1) and IL-5 (Th2) gene expression.
[ "26", "27, 28" ]
Its expression is up-regulated in activated and proliferating T cells and ETS2 is involved in IL-12 p40 (Th1) and IL-5 gene expression.
true
true
true
true
true
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
ETS2 obviously plays a key role in the highly integrated response to YF17D, as it enhanced transcription of several downstream genes that play critical roles in the maturation and differentiation of T cells, B cells, NK cells, and macrophages (Fig.
[ "25", "26", "27", "28", "25", "29" ]
248
41,495
0
false
ETS2 obviously plays a key role in the highly integrated response to YF17D, as it enhanced transcription of several downstream genes that play critical roles in the maturation and differentiation of T cells, B cells, NK cells, and macrophages (Fig.
[]
ETS2 obviously plays a key role in the highly integrated response to YF17D, as it enhanced transcription of several downstream genes that play critical roles in the maturation and differentiation of T cells, B cells, NK cells, and macrophages (Fig.
true
true
true
true
true
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
3, and Fig.
[ "25", "26", "27", "28", "25", "29" ]
11
41,496
0
false
3, and Fig.
[]
3, and Fig.
false
false
true
true
false
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
Indeed, antigen-presenting cell–specific genes that are targets of ETS2 were up-regulated early after vaccination (MARCO, CD86).
[ "25", "26", "27", "28", "25", "29" ]
128
41,497
0
false
Indeed, antigen-presenting cell–specific genes that are targets of ETS2 were up-regulated early after vaccination (MARCO, CD86).
[]
Indeed, antigen-presenting cell–specific genes that are targets of ETS2 were up-regulated early after vaccination (MARCO, CD86).
true
true
true
true
true
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
The role of ETS2 in the early induction of innate immunity is further demonstrated by the increased expression of NK cell receptors, as well as several cytolytic molecules; KIRDL3, PRF1, and GZMB are known targets of members of the Ets family of transcription factors (25, 29).
[ "25", "26", "27", "28", "25", "29" ]
277
41,498
0
false
The role of ETS2 in the early induction of innate immunity is further demonstrated by the increased expression of NK cell receptors, as well as several cytolytic molecules; KIRDL3, PRF1, and GZMB are known targets of members of the Ets family of transcription factors.
[ "25, 29" ]
The role of ETS2 in the early induction of innate immunity is further demonstrated by the increased expression of NK cell receptors, as well as several cytolytic molecules; KIRDL3, PRF1, and GZMB are known targets of members of the Ets family of transcription factors.
true
true
true
true
true
7,155
2
DISCUSSION
1
25
[ "bib25", "bib26", "bib27", "bib28", "bib25", "bib29" ]
19,047,440
pmid-16652219|pmid-2187191|pmid-16518544|pmid-10212281|pmid-16652219|pmid-8413265
Moreover, flow cytometry analysis on PBMCs from vaccinated volunteers revealed that YF17D stimulates the proliferation of several leukocyte populations (Fig.
[ "25", "26", "27", "28", "25", "29" ]
157
41,499
0
false
Moreover, flow cytometry analysis on PBMCs from vaccinated volunteers revealed that YF17D stimulates the proliferation of several leukocyte populations (Fig.
[]
Moreover, flow cytometry analysis on PBMCs from vaccinated volunteers revealed that YF17D stimulates the proliferation of several leukocyte populations (Fig.
true
true
true
true
true
7,155
3
DISCUSSION
1
30
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
Several of the other predicted transcription nodes are likely to play significant roles in the induction of innate and adaptive immunity in response to YF17D.
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
158
41,500
0
false
Several of the other predicted transcription nodes are likely to play significant roles in the induction of innate and adaptive immunity in response to YF17D.
[]
Several of the other predicted transcription nodes are likely to play significant roles in the induction of innate and adaptive immunity in response to YF17D.
true
true
true
true
true
7,156
3
DISCUSSION
1
30
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
IRF1 mediates the antiviral activity of IFNs, similar to IRF7 (30).
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
67
41,501
1
false
IRF1 mediates the antiviral activity of IFNs, similar to IRF7.
[ "30" ]
IRF1 mediates the antiviral activity of IFNs, similar to IRF7.
true
true
true
true
true
7,156
3
DISCUSSION
1
30
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
IRF1 and IRF8 were found to synergistically activate IL-12 p35 and p40 gene expression in macrophages (31–33).
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
110
41,502
0
false
IRF1 and IRF8 were found to synergistically activate IL-12 p35 and p40 gene expression in macrophages.
[ "31–33" ]
IRF1 and IRF8 were found to synergistically activate IL-12 p35 and p40 gene expression in macrophages.
true
true
true
true
true
7,156
3
DISCUSSION
1
34
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
IRF8 also mediates activation of NF-κB upon TLR9 triggering in DCs (34), and IRF8 and LMO2 were both found to be expressed in B cell germinal centers, suggesting a role in the development of the humoral response (35, 36).
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
221
41,503
1
false
IRF8 also mediates activation of NF-κB upon TLR9 triggering in DCs, and IRF8 and LMO2 were both found to be expressed in B cell germinal centers, suggesting a role in the development of the humoral response.
[ "34", "35, 36" ]
IRF8 also mediates activation of NF-κB upon TLR9 triggering in DCs, and IRF8 and LMO2 were both found to be expressed in B cell germinal centers, suggesting a role in the development of the humoral response.
true
true
true
true
true
7,156
3
DISCUSSION
1
37
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
Moreover, TAL1 may be involved in T cell proliferation and differentiation (37).
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
80
41,504
1
false
Moreover, TAL1 may be involved in T cell proliferation and differentiation.
[ "37" ]
Moreover, TAL1 may be involved in T cell proliferation and differentiation.
true
true
true
true
true
7,156
3
DISCUSSION
1
38
[ "bib30", "bib31", "bib33", "bib34", "bib35", "bib36", "bib37", "bib38", "bib39", "bib40" ]
19,047,440
pmid-9865486|pmid-12417340|pmid-15489234|pmid-15153500|pmid-16380510|pmid-17038524|pmid-14651981|pmid-7892279|pmid-18311149|pmid-12149244
E2F4 is involved in cell cycle regulation (38), and FOXO3A and E2F1 are mediators of apoptosis (39, 40).
[ "30", "31", "33", "34", "35", "36", "37", "38", "39", "40" ]
104
41,505
1
false
E2F4 is involved in cell cycle regulation, and FOXO3A and E2F1 are mediators of apoptosis.
[ "38", "39, 40" ]
E2F4 is involved in cell cycle regulation, and FOXO3A and E2F1 are mediators of apoptosis.
true
true
true
true
true
7,156
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
A common feature of these masterswitch genes is that they control the induction of several pathways of the innate immune response, including type I IFNs, but also the inflammasome and complement.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
195
41,506
0
false
A common feature of these masterswitch genes is that they control the induction of several pathways of the innate immune response, including type I IFNs, but also the inflammasome and complement.
[]
A common feature of these masterswitch genes is that they control the induction of several pathways of the innate immune response, including type I IFNs, but also the inflammasome and complement.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
The inflammasome is a large protein assembly that includes pyrin- and CARD domain–containing adaptor proteins complexed with cysteine proteinases known as caspases.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
164
41,507
0
false
The inflammasome is a large protein assembly that includes pyrin- and CARD domain–containing adaptor proteins complexed with cysteine proteinases known as caspases.
[]
The inflammasome is a large protein assembly that includes pyrin- and CARD domain–containing adaptor proteins complexed with cysteine proteinases known as caspases.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
It mediates the processing and activation of these caspases, and subsequently the cleavage and secretion of the proinflammatory cytokines IL-1β and -18 (41).
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
157
41,508
1
false
It mediates the processing and activation of these caspases, and subsequently the cleavage and secretion of the proinflammatory cytokines IL-1β and -18.
[ "41" ]
It mediates the processing and activation of these caspases, and subsequently the cleavage and secretion of the proinflammatory cytokines IL-1β and -18.
true
true
true
true
true
7,157
4
DISCUSSION
1
42
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Activation of the inflammasome has been recently shown to be induced by adenoviruses (42), and to be a key event for the successful adjuvant effect of alum, one of the only two adjuvants licensed to be used in humans (43).
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
222
41,509
1
false
Activation of the inflammasome has been recently shown to be induced by adenoviruses, and to be a key event for the successful adjuvant effect of alum, one of the only two adjuvants licensed to be used in humans.
[ "42", "43" ]
Activation of the inflammasome has been recently shown to be induced by adenoviruses, and to be a key event for the successful adjuvant effect of alum, one of the only two adjuvants licensed to be used in humans.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Our gene array results demonstrate that two components of the inflammasome, caspase-1 and -5, were up-regulated after YF17D vaccination, in volunteers from the Montreal cohort, whereas only caspase-1 is up-regulated in volunteers from the Lausanne cohort (Fig.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
260
41,510
0
false
Our gene array results demonstrate that two components of the inflammasome, caspase-1 and -5, were up-regulated after YF17D vaccination, in volunteers from the Montreal cohort, whereas only caspase-1 is up-regulated in volunteers from the Lausanne cohort (Fig.
[]
Our gene array results demonstrate that two components of the inflammasome, caspase-1 and -5, were up-regulated after YF17D vaccination, in volunteers from the Montreal cohort, whereas only caspase-1 is up-regulated in volunteers from the Lausanne cohort (Fig.
true
true
true
true
true
7,157
4
DISCUSSION
1
44
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
There are different kinds of inflammasomes, each containing a specific combination of inflammatory caspases and adaptor molecules (44).
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
135
41,511
1
false
There are different kinds of inflammasomes, each containing a specific combination of inflammatory caspases and adaptor molecules.
[ "44" ]
There are different kinds of inflammasomes, each containing a specific combination of inflammatory caspases and adaptor molecules.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Hence, NALP1 inflammasomes contain ASC, NALP1, caspase-1, and caspase-5, whereas NALP3 inflammasomes contain ASC, NALP3, and caspase-1 (no caspase-5).
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
150
41,512
0
false
Hence, NALP1 inflammasomes contain ASC, NALP1, caspase-1, and caspase-5, whereas NALP3 inflammasomes contain ASC, NALP3, and caspase-1 (no caspase-5).
[]
Hence, NALP1 inflammasomes contain ASC, NALP1, caspase-1, and caspase-5, whereas NALP3 inflammasomes contain ASC, NALP3, and caspase-1 (no caspase-5).
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
It is possible that NALP1 inflammasomes were not induced in volunteers from the Lausanne cohort, whereas NALP3 inflammasomes were induced in both cohorts.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
154
41,513
0
false
It is possible that NALP1 inflammasomes were not induced in volunteers from the Lausanne cohort, whereas NALP3 inflammasomes were induced in both cohorts.
[]
It is possible that NALP1 inflammasomes were not induced in volunteers from the Lausanne cohort, whereas NALP3 inflammasomes were induced in both cohorts.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Interestingly, our gene array data also show a modulation of genes that are involved in IL-1β signaling, IL-1R2 and IL-1RN.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
123
41,514
0
false
Interestingly, our gene array data also show a modulation of genes that are involved in IL-1β signaling, IL-1R2 and IL-1RN.
[]
Interestingly, our gene array data also show a modulation of genes that are involved in IL-1β signaling, IL-1R2 and IL-1RN.
true
true
true
true
true
7,157
4
DISCUSSION
1
45
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
IL-1R2, which is a decoy receptor that competes with the binding of IL-1β to its receptor (45), is down-regulated, further confirming increased inflammasome activities and IL-1β processing and secretion after immunization with YF17D.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
233
41,515
1
false
IL-1R2, which is a decoy receptor that competes with the binding of IL-1β to its receptor, is down-regulated, further confirming increased inflammasome activities and IL-1β processing and secretion after immunization with YF17D.
[ "45" ]
IL-1R2, which is a decoy receptor that competes with the binding of IL-1β to its receptor, is down-regulated, further confirming increased inflammasome activities and IL-1β processing and secretion after immunization with YF17D.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
The gene encoding IL-1RN, a cytokine that is an IL-1R antagonist, is up-regulated upon vaccination; this increase could be part of a negative feedback loop or could be caused by the up-regulation of STAT1 and STAT2, two transcription factors that induce IL-1RN up-regulation (46, 47).
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
284
41,516
0
false
The gene encoding IL-1RN, a cytokine that is an IL-1R antagonist, is up-regulated upon vaccination; this increase could be part of a negative feedback loop or could be caused by the up-regulation of STAT1 and STAT2, two transcription factors that induce IL-1RN up-regulation.
[ "46, 47" ]
The gene encoding IL-1RN, a cytokine that is an IL-1R antagonist, is up-regulated upon vaccination; this increase could be part of a negative feedback loop or could be caused by the up-regulation of STAT1 and STAT2, two transcription factors that induce IL-1RN up-regulation.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Activation of the inflammasome by YF17D was confirmed at the protein level by incubating immature DCs with the virus and assessing their IL-1β secretion.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
153
41,517
0
false
Activation of the inflammasome by YF17D was confirmed at the protein level by incubating immature DCs with the virus and assessing their IL-1β secretion.
[]
Activation of the inflammasome by YF17D was confirmed at the protein level by incubating immature DCs with the virus and assessing their IL-1β secretion.
true
true
true
true
true
7,157
4
DISCUSSION
1
41
[ "bib41", "bib42", "bib43", "bib44", "bib45", "bib46", "bib47", "bib43", "bib48" ]
19,047,440
pmid-17977705|pmid-18288107|pmid-18566365|pmid-15163405|pmid-11377293|pmid-18494930|pmid-11158047|pmid-18566365|pmid-17570108
Representative results of ex vivo experiments performed on cells from a minimum of three individuals are shown in Fig.
[ "41", "42", "43", "44", "45", "46", "47", "43", "48" ]
118
41,518
0
false
Representative results of ex vivo experiments performed on cells from a minimum of three individuals are shown in Fig.
[]
Representative results of ex vivo experiments performed on cells from a minimum of three individuals are shown in Fig.
true
true
true
true
true
7,157