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PMC1156954_F3_2324.jpg | What is the principal component of this image? | Ultrastructural characteristics of a Coronavirus-Infected cell in BAL fluid from a SARS patient at 60 days, with several intracellular particles. The virions are indicated by the arrowheads in Panel A. Panel B shows the area indicated by the asterisk in Panel A at higher magnification. The bar in Panel A (500 nm) and Panel B (100 nm) is indicated. |
PMC1156956_F2_2330.jpg | What is being portrayed in this visual content? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2325.jpg | What is shown in this image? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2332.jpg | What is the main focus of this visual representation? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2334.jpg | Can you identify the primary element in this image? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2329.jpg | What object or scene is depicted here? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2331.jpg | What is the core subject represented in this visual? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2336.jpg | What is the focal point of this photograph? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2328.jpg | What is shown in this image? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2326.jpg | What is the dominant medical problem in this image? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1156956_F2_2327.jpg | What is the central feature of this picture? | Expression of SPHK1 in PCLS from different mouse strains studied via confocal laser scanning microscopy. Representative confocal laser scanning micrographs of double labeling immunohistochemistry demonstrates the restriction of SPHK1-immunoreactivity to smooth muscle cells, identified by immunoreactivity for the marker protein α-smooth muscle actin (α-sma). SPHK1a-immunoreactivity was present in peripheral airway smooth muscle of knock-out animals (M2R-KO, a-d) and wild-type (M2R-wt, e-h, FVB, i-l). Granular immunoreactivity could be detected in the cytoplasm and in the membrane of smooth muscle cells. Bars = 50 μm |
PMC1159039_F2_2348.jpg | What is the principal component of this image? | Simultaneous capture of GFP and CFP. Stable cell lines expressing either GFP or CFP were co-cultured (left panels) or a stable cell line expressing CFP and GFP-CAAX (membrane targeted – middle panels) or a cell line expressing CFP transiently transfected with GFP-ZYXIN (right panels) were analyzed. Top panels show the fluorescence captured using a 450/80 filter and non-descanned detection. Upper-mid panels show the fluorescence captured using a 515/30 filter and non-descanned detection. Lower-mid panels show the result of subtracting the 450/80 signal from the 515/30 signal. Bottom panels show a merge of the top panels (CFP signal in blue) and the lower-mid panels (GFP signal in green). GFP-CAAX, which generally targets to the membrane can be seen labeling of intracellular structures in the merge and GFP images (middle panel; arrow). All images were taken with a 40× objective and a final magnification of 500× for the right 2 columns and 1000× for the left. Scale bar = 25 μm for the right 2 columns and 10 μm for the left. |
PMC1159039_F2_2354.jpg | Can you identify the primary element in this image? | Simultaneous capture of GFP and CFP. Stable cell lines expressing either GFP or CFP were co-cultured (left panels) or a stable cell line expressing CFP and GFP-CAAX (membrane targeted – middle panels) or a cell line expressing CFP transiently transfected with GFP-ZYXIN (right panels) were analyzed. Top panels show the fluorescence captured using a 450/80 filter and non-descanned detection. Upper-mid panels show the fluorescence captured using a 515/30 filter and non-descanned detection. Lower-mid panels show the result of subtracting the 450/80 signal from the 515/30 signal. Bottom panels show a merge of the top panels (CFP signal in blue) and the lower-mid panels (GFP signal in green). GFP-CAAX, which generally targets to the membrane can be seen labeling of intracellular structures in the merge and GFP images (middle panel; arrow). All images were taken with a 40× objective and a final magnification of 500× for the right 2 columns and 1000× for the left. Scale bar = 25 μm for the right 2 columns and 10 μm for the left. |
PMC1159039_F2_2352.jpg | What is the principal component of this image? | Simultaneous capture of GFP and CFP. Stable cell lines expressing either GFP or CFP were co-cultured (left panels) or a stable cell line expressing CFP and GFP-CAAX (membrane targeted – middle panels) or a cell line expressing CFP transiently transfected with GFP-ZYXIN (right panels) were analyzed. Top panels show the fluorescence captured using a 450/80 filter and non-descanned detection. Upper-mid panels show the fluorescence captured using a 515/30 filter and non-descanned detection. Lower-mid panels show the result of subtracting the 450/80 signal from the 515/30 signal. Bottom panels show a merge of the top panels (CFP signal in blue) and the lower-mid panels (GFP signal in green). GFP-CAAX, which generally targets to the membrane can be seen labeling of intracellular structures in the merge and GFP images (middle panel; arrow). All images were taken with a 40× objective and a final magnification of 500× for the right 2 columns and 1000× for the left. Scale bar = 25 μm for the right 2 columns and 10 μm for the left. |
PMC1159039_F2_2350.jpg | What is the principal component of this image? | Simultaneous capture of GFP and CFP. Stable cell lines expressing either GFP or CFP were co-cultured (left panels) or a stable cell line expressing CFP and GFP-CAAX (membrane targeted – middle panels) or a cell line expressing CFP transiently transfected with GFP-ZYXIN (right panels) were analyzed. Top panels show the fluorescence captured using a 450/80 filter and non-descanned detection. Upper-mid panels show the fluorescence captured using a 515/30 filter and non-descanned detection. Lower-mid panels show the result of subtracting the 450/80 signal from the 515/30 signal. Bottom panels show a merge of the top panels (CFP signal in blue) and the lower-mid panels (GFP signal in green). GFP-CAAX, which generally targets to the membrane can be seen labeling of intracellular structures in the merge and GFP images (middle panel; arrow). All images were taken with a 40× objective and a final magnification of 500× for the right 2 columns and 1000× for the left. Scale bar = 25 μm for the right 2 columns and 10 μm for the left. |
PMC1159039_F2_2356.jpg | What is the focal point of this photograph? | Simultaneous capture of GFP and CFP. Stable cell lines expressing either GFP or CFP were co-cultured (left panels) or a stable cell line expressing CFP and GFP-CAAX (membrane targeted – middle panels) or a cell line expressing CFP transiently transfected with GFP-ZYXIN (right panels) were analyzed. Top panels show the fluorescence captured using a 450/80 filter and non-descanned detection. Upper-mid panels show the fluorescence captured using a 515/30 filter and non-descanned detection. Lower-mid panels show the result of subtracting the 450/80 signal from the 515/30 signal. Bottom panels show a merge of the top panels (CFP signal in blue) and the lower-mid panels (GFP signal in green). GFP-CAAX, which generally targets to the membrane can be seen labeling of intracellular structures in the merge and GFP images (middle panel; arrow). All images were taken with a 40× objective and a final magnification of 500× for the right 2 columns and 1000× for the left. Scale bar = 25 μm for the right 2 columns and 10 μm for the left. |
PMC1159039_F3_2339.jpg | What stands out most in this visual? | Simultaneous capture of GFP, CFP and collagen second harmonic fluorescence in a living tissue. The panels on the left-hand side show images of an experimentally generated mammary tumour creating by injecting a mixture of cells either expressing GFP or CFP. The panels on the right-hand side show images of an experimentally generated mammary tumour with cells expressing CFP to mark the entire cytoplasmic volume and GFP-CAAX (membrane targeted). 880 nm laser light was used to excite the all the samples and the fluorescence was captured with indicated filters using non-descanned detection. All images were taken with a 40× objective and a final magnification of 500×. Scale bar = 25 μm. |
PMC1159039_F3_2341.jpg | What is the core subject represented in this visual? | Simultaneous capture of GFP, CFP and collagen second harmonic fluorescence in a living tissue. The panels on the left-hand side show images of an experimentally generated mammary tumour creating by injecting a mixture of cells either expressing GFP or CFP. The panels on the right-hand side show images of an experimentally generated mammary tumour with cells expressing CFP to mark the entire cytoplasmic volume and GFP-CAAX (membrane targeted). 880 nm laser light was used to excite the all the samples and the fluorescence was captured with indicated filters using non-descanned detection. All images were taken with a 40× objective and a final magnification of 500×. Scale bar = 25 μm. |
PMC1159039_F3_2343.jpg | What stands out most in this visual? | Simultaneous capture of GFP, CFP and collagen second harmonic fluorescence in a living tissue. The panels on the left-hand side show images of an experimentally generated mammary tumour creating by injecting a mixture of cells either expressing GFP or CFP. The panels on the right-hand side show images of an experimentally generated mammary tumour with cells expressing CFP to mark the entire cytoplasmic volume and GFP-CAAX (membrane targeted). 880 nm laser light was used to excite the all the samples and the fluorescence was captured with indicated filters using non-descanned detection. All images were taken with a 40× objective and a final magnification of 500×. Scale bar = 25 μm. |
PMC1159039_F3_2342.jpg | What key item or scene is captured in this photo? | Simultaneous capture of GFP, CFP and collagen second harmonic fluorescence in a living tissue. The panels on the left-hand side show images of an experimentally generated mammary tumour creating by injecting a mixture of cells either expressing GFP or CFP. The panels on the right-hand side show images of an experimentally generated mammary tumour with cells expressing CFP to mark the entire cytoplasmic volume and GFP-CAAX (membrane targeted). 880 nm laser light was used to excite the all the samples and the fluorescence was captured with indicated filters using non-descanned detection. All images were taken with a 40× objective and a final magnification of 500×. Scale bar = 25 μm. |
PMC1159039_F3_2344.jpg | What is the dominant medical problem in this image? | Simultaneous capture of GFP, CFP and collagen second harmonic fluorescence in a living tissue. The panels on the left-hand side show images of an experimentally generated mammary tumour creating by injecting a mixture of cells either expressing GFP or CFP. The panels on the right-hand side show images of an experimentally generated mammary tumour with cells expressing CFP to mark the entire cytoplasmic volume and GFP-CAAX (membrane targeted). 880 nm laser light was used to excite the all the samples and the fluorescence was captured with indicated filters using non-descanned detection. All images were taken with a 40× objective and a final magnification of 500×. Scale bar = 25 μm. |
PMC1159039_F4_2358.jpg | What key item or scene is captured in this photo? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2363.jpg | What is the dominant medical problem in this image? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2364.jpg | What can you see in this picture? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2362.jpg | What is the focal point of this photograph? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2361.jpg | What is the central feature of this picture? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2366.jpg | What is shown in this image? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1159039_F4_2365.jpg | What is the central feature of this picture? | Differences in motility and invasion can be determined by imaging cells with different metastatic potential in the same tumor. A) The panels show a time series of images of an experimentally generated mammary tumour with cells either expressing GFP (low metastatic in green) or CFP (high metastatic in white) with collagen fibres in purple. Arrows point to moving CFP cells and arrowheads to moving GFP cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 1). Images were taken with a 20× objective and a final magnification of 250×. B) CFP-labeled cells (white) and control GFP labelled cells (green) are seen in the lung after extravasation and metastatic growth. Images were taken with a 20× objective and a final magnification of 250×. C) Left hand panel CFP cell (white) has a filopod (arrow) protruding into a field of GFP control cells (green). Right hand panel CFP cell (white) has a lamellapod (arrow) seen in the middle of a field of GFP control cells (green). Supplementary movie shows a lamellapod retracting over time (Additional file 2). Images were taken with a 60× objective and a final magnification of 1000×. D) The panels show a time series of high magnification images of an experimentally generated mammary tumor with cells either expressing GFP (in green) or CFP (in white) with collagen fibers in purple. Moving high metastatic cells are outlined in white and an orange arrow shows the path taken by both cells. Supplementary movie shows this image sequence including intermediate frames (Additional file 3). Images were taken with a 40× objective and a final magnification of 500×. Scale bar for A-E = 25 um. |
PMC1164403_F1_2375.jpg | What is the core subject represented in this visual? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2367.jpg | What's the most prominent thing you notice in this picture? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2372.jpg | What is being portrayed in this visual content? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2374.jpg | What is the main focus of this visual representation? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2368.jpg | Describe the main subject of this image. | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2373.jpg | What stands out most in this visual? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F1_2371.jpg | What is the central feature of this picture? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in normal glandular tissues. Mucosa of the corpus of stomach (a, b; magnification × 10) and (c, d; magnification × 45). Colonic mucosa (e, f; magnification × 25), (g; magnification × 60) and (h; magnification × 50). Prostatic glands (i; magnification × 60) and (j; magnification × 50). |
PMC1164403_F2_2382.jpg | What does this image primarily show? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2386.jpg | What does this image primarily show? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2379.jpg | What's the most prominent thing you notice in this picture? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2385.jpg | What can you see in this picture? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2380.jpg | What does this image primarily show? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2381.jpg | What is the principal component of this image? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2377.jpg | What stands out most in this visual? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164403_F2_2384.jpg | What is the dominant medical problem in this image? | Examples of the different immunostaining patterns obtained using the antibody clones 22-1-1 and Ab-1 in various adenocarcinomas. Signet ring cell gastric carcinoma (a, b; magnification × 80). Colorectal adenocarcinoma (c, d; magnification × 60). Lymph node metastasis (e, f; magnification × 50). Prostatic adenocarcinoma (g; magnification × 60) and (h; magnification × 50): Adenocarcinoma of the lung (i, j; magnification × 60). |
PMC1164415_F1_2387.jpg | What stands out most in this visual? | Type I Gaucher disease with exophthalmos and pulmonary arteriovenous malformation. (A) MRI of the head shows increases in retrobulbar space and hypertrophy of extra-ocular muscles. (B) Chest X ray reveals prominent pulmonary conus and increased infiltration over right lower lung field. (C) High-resolution computed tomography of chest reveals engorged right inferior pulmonary artery and its draining veins. (D) Pulmonary angiogram demonstrates right pulmonary arteriovenous malformation. |
PMC1164415_F1_2389.jpg | What is the core subject represented in this visual? | Type I Gaucher disease with exophthalmos and pulmonary arteriovenous malformation. (A) MRI of the head shows increases in retrobulbar space and hypertrophy of extra-ocular muscles. (B) Chest X ray reveals prominent pulmonary conus and increased infiltration over right lower lung field. (C) High-resolution computed tomography of chest reveals engorged right inferior pulmonary artery and its draining veins. (D) Pulmonary angiogram demonstrates right pulmonary arteriovenous malformation. |
PMC1164418_F2_2395.jpg | What object or scene is depicted here? | The same patient as in Fig. 1: The Artifact due to dental mass (Fig. 1) is not visible in the CT-corrected images with segmentation of transmission data. |
PMC1164418_F2_2396.jpg | What stands out most in this visual? | The same patient as in Fig. 1: The Artifact due to dental mass (Fig. 1) is not visible in the CT-corrected images with segmentation of transmission data. |
PMC1164418_F4_2391.jpg | What is the principal component of this image? | The same patient as in Fig. 3: There is no pathological uptake in the periprosthetic area of the right hip in the CT-corrected images with segmentation of transmission data. |
PMC1164418_F4_2392.jpg | What is being portrayed in this visual content? | The same patient as in Fig. 3: There is no pathological uptake in the periprosthetic area of the right hip in the CT-corrected images with segmentation of transmission data. |
PMC1164436_F1_2398.jpg | What is the main focus of this visual representation? | Chest X-ray and Computerized tomographic scan showing multiple metastatic nodules in the lung |
PMC1164436_F1_2397.jpg | What object or scene is depicted here? | Chest X-ray and Computerized tomographic scan showing multiple metastatic nodules in the lung |
PMC1166561_F1_2409.jpg | What is the principal component of this image? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2401.jpg | What is shown in this image? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2407.jpg | What object or scene is depicted here? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2406.jpg | Can you identify the primary element in this image? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2405.jpg | What object or scene is depicted here? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2400.jpg | What is the core subject represented in this visual? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2399.jpg | What is the principal component of this image? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166561_F1_2404.jpg | What stands out most in this visual? | OIS response to low vs. high amplitude stimulation. OIS images taken from three subjects (A, B, C). All images are anatomically oriented as shown in the top left image. Images were obtained by averaging across 10 experimental trials and then summing frames taken from the time of stimulus onset to 5 seconds after stimulus offset to better show regions of high absorbance indicated by dark pixels. The left column shows responses acquired in the absence of stimulation (control), while the middle and right columns show the stimulus-evoked response to 50 and 400 μm respectively in the same subjects. |
PMC1166574_F3_2420.jpg | What stands out most in this visual? | Immunohistochemistry of TGF-β1 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F3_2418.jpg | What is the main focus of this visual representation? | Immunohistochemistry of TGF-β1 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F3_2416.jpg | What object or scene is depicted here? | Immunohistochemistry of TGF-β1 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F3_2419.jpg | What is the core subject represented in this visual? | Immunohistochemistry of TGF-β1 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F3_2421.jpg | What is the focal point of this photograph? | Immunohistochemistry of TGF-β1 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F4_2415.jpg | What key item or scene is captured in this photo? | Immunohistochemistry of TGF-β2 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F4_2411.jpg | What is the focal point of this photograph? | Immunohistochemistry of TGF-β2 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F4_2410.jpg | What is the focal point of this photograph? | Immunohistochemistry of TGF-β2 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F4_2412.jpg | What stands out most in this visual? | Immunohistochemistry of TGF-β2 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F4_2413.jpg | What is the dominant medical problem in this image? | Immunohistochemistry of TGF-β2 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per day of pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F5_2425.jpg | What is being portrayed in this visual content? | Immunohistochemistry of TGF-β3 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per of day pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F5_2422.jpg | What can you see in this picture? | Immunohistochemistry of TGF-β3 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per of day pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F5_2426.jpg | What is the core subject represented in this visual? | Immunohistochemistry of TGF-β3 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per of day pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166574_F5_2423.jpg | What is shown in this image? | Immunohistochemistry of TGF-β3 in rat endometrium during pregnancy. IHC shown are from one representative experiment and were repeated 6 times using 6 different uterine sections from 6 different rats per of day pregnancy. Representative days of pregnancy are presented (A: day 4; B: day 5.5; C: day 6.5; D: day 10; E: day 14; F: negative control in which primary antibody was absent). le: luminal epithelium; em: embryo; s: stroma. Magnification: 40×. |
PMC1166581_F1_2428.jpg | Describe the main subject of this image. | Computed tomography scanner showing a large left-pararenal mass (Hyalin-vascular type of Castleman's disease). Note the presence of microcalcification (white spot) within the mass. |
PMC1173139_F1_2431.jpg | What is the main focus of this visual representation? | Posterior-anterior view of a chest X-ray film demonstrated a huge mass shadow in the left upper lung field. |
PMC1173139_F3_2432.jpg | What stands out most in this visual? | Computerised tomographic scan before and after treatment. A) Abdominal CT showed multiple lymph node swelling, suggesting the presence of metastases (arrowhead). B) Abdominal CT showed the lymph nodes metastases to have completely disappeared. |
PMC1173139_F3_2433.jpg | What is the dominant medical problem in this image? | Computerised tomographic scan before and after treatment. A) Abdominal CT showed multiple lymph node swelling, suggesting the presence of metastases (arrowhead). B) Abdominal CT showed the lymph nodes metastases to have completely disappeared. |
PMC1174869_F3_2434.jpg | Can you identify the primary element in this image? | X-ray of abdomen 2 years prior showing outline of plastic soda bottle in sigmoid colon (arrows). |
PMC1174941_F3_2440.jpg | What object or scene is depicted here? | Peptidyl arginine deiminase 4 (PAD4) is present in the arthritic joint. PAD4 was detected in infiltrating cells (a), localised to the cytoplasm of mononuclear cells (c,e). Unimmunised animals were negative for PAD4 staining (f). Immunohistochemical stainings were performed with a rabbit anti-PAD4 antibody. Control staining was performed with preimmune rabbit sera (b,d) (Original magnifications: ×40 (a,b,f); ×200 (c-e)). |
PMC1174941_F3_2435.jpg | What can you see in this picture? | Peptidyl arginine deiminase 4 (PAD4) is present in the arthritic joint. PAD4 was detected in infiltrating cells (a), localised to the cytoplasm of mononuclear cells (c,e). Unimmunised animals were negative for PAD4 staining (f). Immunohistochemical stainings were performed with a rabbit anti-PAD4 antibody. Control staining was performed with preimmune rabbit sera (b,d) (Original magnifications: ×40 (a,b,f); ×200 (c-e)). |
PMC1174941_F3_2439.jpg | What can you see in this picture? | Peptidyl arginine deiminase 4 (PAD4) is present in the arthritic joint. PAD4 was detected in infiltrating cells (a), localised to the cytoplasm of mononuclear cells (c,e). Unimmunised animals were negative for PAD4 staining (f). Immunohistochemical stainings were performed with a rabbit anti-PAD4 antibody. Control staining was performed with preimmune rabbit sera (b,d) (Original magnifications: ×40 (a,b,f); ×200 (c-e)). |
PMC1174941_F3_2437.jpg | What object or scene is depicted here? | Peptidyl arginine deiminase 4 (PAD4) is present in the arthritic joint. PAD4 was detected in infiltrating cells (a), localised to the cytoplasm of mononuclear cells (c,e). Unimmunised animals were negative for PAD4 staining (f). Immunohistochemical stainings were performed with a rabbit anti-PAD4 antibody. Control staining was performed with preimmune rabbit sera (b,d) (Original magnifications: ×40 (a,b,f); ×200 (c-e)). |
PMC1174952_F1_2443.jpg | What is the principal component of this image? | The three-dimensional poly(lactic-glycolic acid) (3D-PLGA) scaffold. The micropore side (cell seeding side) (a) and a cross section (b) of the scaffold. Schematic illustration of cell seeding (left) and scanning electron photomicrograph of cross section of cells seeded in the 3D-PLGA scaffold (right) (c). The cells lie in a uniform array at the palisades, similar to hyaline cartilage. Gross appearance of a cartilage defect in the patella groove implanted with a complex between adherent bone marrow cells and 3D-PLGA scaffold (d). The arrows indicate cell/PLGA scaffold. |
PMC1174952_F1_2441.jpg | What key item or scene is captured in this photo? | The three-dimensional poly(lactic-glycolic acid) (3D-PLGA) scaffold. The micropore side (cell seeding side) (a) and a cross section (b) of the scaffold. Schematic illustration of cell seeding (left) and scanning electron photomicrograph of cross section of cells seeded in the 3D-PLGA scaffold (right) (c). The cells lie in a uniform array at the palisades, similar to hyaline cartilage. Gross appearance of a cartilage defect in the patella groove implanted with a complex between adherent bone marrow cells and 3D-PLGA scaffold (d). The arrows indicate cell/PLGA scaffold. |
PMC1175028_F1_2444.jpg | What is the principal component of this image? | Dynamic gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA)-enhanced MRI of the wrist in a patient with psoriatic arthritis. Sequence (a) shows the precontrast image; sequences (b–d) show images acquired after 36, 90, and 180 s, respectively. The region of interest on which the enhancement curve has been calculated is outlined. Gd-DTPA, dynamic gadolinium-diethylenetriamine pentaacetic acid; MRI, magnetic resonance image. |
PMC1175028_F1_2446.jpg | What stands out most in this visual? | Dynamic gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA)-enhanced MRI of the wrist in a patient with psoriatic arthritis. Sequence (a) shows the precontrast image; sequences (b–d) show images acquired after 36, 90, and 180 s, respectively. The region of interest on which the enhancement curve has been calculated is outlined. Gd-DTPA, dynamic gadolinium-diethylenetriamine pentaacetic acid; MRI, magnetic resonance image. |
PMC1175028_F1_2445.jpg | What is the dominant medical problem in this image? | Dynamic gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA)-enhanced MRI of the wrist in a patient with psoriatic arthritis. Sequence (a) shows the precontrast image; sequences (b–d) show images acquired after 36, 90, and 180 s, respectively. The region of interest on which the enhancement curve has been calculated is outlined. Gd-DTPA, dynamic gadolinium-diethylenetriamine pentaacetic acid; MRI, magnetic resonance image. |
PMC1175028_F1_2447.jpg | What can you see in this picture? | Dynamic gadolinium-diethylenetriamine pentaacetic acid (Gd-DTPA)-enhanced MRI of the wrist in a patient with psoriatic arthritis. Sequence (a) shows the precontrast image; sequences (b–d) show images acquired after 36, 90, and 180 s, respectively. The region of interest on which the enhancement curve has been calculated is outlined. Gd-DTPA, dynamic gadolinium-diethylenetriamine pentaacetic acid; MRI, magnetic resonance image. |
PMC1175035_F3_2448.jpg | What is the principal component of this image? | Peripheral quantitative computed tomography (pQCT) scans of one representative mouse in each group. Trabecular bone mineral density (BMD) was determined with a metaphyseal scan at a point 3% of the length of the femur from the growth plate and the inner 45% of the area was defined as the trabecular bone compartment. (a) Sham-operated control; (b) ovariectomy control; (c) sham-operated, arthritic mouse; (d) ovariectomized, arthritic mouse. The bar shows the density of the bone, from 0 (black) to 750 mg/cm3 (white). |
PMC1175035_F3_2449.jpg | What object or scene is depicted here? | Peripheral quantitative computed tomography (pQCT) scans of one representative mouse in each group. Trabecular bone mineral density (BMD) was determined with a metaphyseal scan at a point 3% of the length of the femur from the growth plate and the inner 45% of the area was defined as the trabecular bone compartment. (a) Sham-operated control; (b) ovariectomy control; (c) sham-operated, arthritic mouse; (d) ovariectomized, arthritic mouse. The bar shows the density of the bone, from 0 (black) to 750 mg/cm3 (white). |
PMC1175035_F3_2451.jpg | What can you see in this picture? | Peripheral quantitative computed tomography (pQCT) scans of one representative mouse in each group. Trabecular bone mineral density (BMD) was determined with a metaphyseal scan at a point 3% of the length of the femur from the growth plate and the inner 45% of the area was defined as the trabecular bone compartment. (a) Sham-operated control; (b) ovariectomy control; (c) sham-operated, arthritic mouse; (d) ovariectomized, arthritic mouse. The bar shows the density of the bone, from 0 (black) to 750 mg/cm3 (white). |
PMC1175035_F3_2450.jpg | What can you see in this picture? | Peripheral quantitative computed tomography (pQCT) scans of one representative mouse in each group. Trabecular bone mineral density (BMD) was determined with a metaphyseal scan at a point 3% of the length of the femur from the growth plate and the inner 45% of the area was defined as the trabecular bone compartment. (a) Sham-operated control; (b) ovariectomy control; (c) sham-operated, arthritic mouse; (d) ovariectomized, arthritic mouse. The bar shows the density of the bone, from 0 (black) to 750 mg/cm3 (white). |
PMC1175055_F3_2453.jpg | What key item or scene is captured in this photo? | Immunohistochemical analysis of SDF1 and its receptor. Imunohistochemical staining of (a,b) SDF-1 and (c,d) the SDF-1 receptor CXCR4 in mammary tissues. The left panels show normal tissues, and the right panels show breast tumour tissues. CXCR, CXC chemokine receptor; SDF, stromal cell-derived factor. |
PMC1175055_F3_2452.jpg | What does this image primarily show? | Immunohistochemical analysis of SDF1 and its receptor. Imunohistochemical staining of (a,b) SDF-1 and (c,d) the SDF-1 receptor CXCR4 in mammary tissues. The left panels show normal tissues, and the right panels show breast tumour tissues. CXCR, CXC chemokine receptor; SDF, stromal cell-derived factor. |
PMC1175055_F3_2455.jpg | What key item or scene is captured in this photo? | Immunohistochemical analysis of SDF1 and its receptor. Imunohistochemical staining of (a,b) SDF-1 and (c,d) the SDF-1 receptor CXCR4 in mammary tissues. The left panels show normal tissues, and the right panels show breast tumour tissues. CXCR, CXC chemokine receptor; SDF, stromal cell-derived factor. |
PMC1175055_F3_2454.jpg | What object or scene is depicted here? | Immunohistochemical analysis of SDF1 and its receptor. Imunohistochemical staining of (a,b) SDF-1 and (c,d) the SDF-1 receptor CXCR4 in mammary tissues. The left panels show normal tissues, and the right panels show breast tumour tissues. CXCR, CXC chemokine receptor; SDF, stromal cell-derived factor. |
PMC1175070_F1_2464.jpg | What is the core subject represented in this visual? | Tumor cell diapedesis through human microvascular endothelial cell (HMVEC) monolayers. HBL100 cells were pre-labeled with dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine percholate (DiI; red), co-cultured with HMVEC monolayers for various time periods, fixed with paraformaldehyde and labeled for F-actin (green). Optical sections obtained by laser scanning confocal microscopy at the focal levels indicated in (a,e,i) identified three major stages of diapedesis: round on top of (a-d), migrating through (e-h), or located underneath (i-l) the endothelium. (b) Cells round on top had filopodial extensions present on the apical surface of a cell (arrows). (g) Thick bundles of F-actin were present at the interface between endothelial cells and the migrating tumor cell (arrow). (h) Migrating tumor cells with portions spread underneath the endothelium (arrows) and (i) those that completed diapedesis often contained prominent stress fibers underneath the endothelium (arrows). No major morphological differences between wild-type and connexin43 (Cx43) expressing HBL100 cells were observed. Bar = 10 μm. |
PMC1175070_F1_2462.jpg | What stands out most in this visual? | Tumor cell diapedesis through human microvascular endothelial cell (HMVEC) monolayers. HBL100 cells were pre-labeled with dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine percholate (DiI; red), co-cultured with HMVEC monolayers for various time periods, fixed with paraformaldehyde and labeled for F-actin (green). Optical sections obtained by laser scanning confocal microscopy at the focal levels indicated in (a,e,i) identified three major stages of diapedesis: round on top of (a-d), migrating through (e-h), or located underneath (i-l) the endothelium. (b) Cells round on top had filopodial extensions present on the apical surface of a cell (arrows). (g) Thick bundles of F-actin were present at the interface between endothelial cells and the migrating tumor cell (arrow). (h) Migrating tumor cells with portions spread underneath the endothelium (arrows) and (i) those that completed diapedesis often contained prominent stress fibers underneath the endothelium (arrows). No major morphological differences between wild-type and connexin43 (Cx43) expressing HBL100 cells were observed. Bar = 10 μm. |
PMC1175070_F1_2468.jpg | What can you see in this picture? | Tumor cell diapedesis through human microvascular endothelial cell (HMVEC) monolayers. HBL100 cells were pre-labeled with dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine percholate (DiI; red), co-cultured with HMVEC monolayers for various time periods, fixed with paraformaldehyde and labeled for F-actin (green). Optical sections obtained by laser scanning confocal microscopy at the focal levels indicated in (a,e,i) identified three major stages of diapedesis: round on top of (a-d), migrating through (e-h), or located underneath (i-l) the endothelium. (b) Cells round on top had filopodial extensions present on the apical surface of a cell (arrows). (g) Thick bundles of F-actin were present at the interface between endothelial cells and the migrating tumor cell (arrow). (h) Migrating tumor cells with portions spread underneath the endothelium (arrows) and (i) those that completed diapedesis often contained prominent stress fibers underneath the endothelium (arrows). No major morphological differences between wild-type and connexin43 (Cx43) expressing HBL100 cells were observed. Bar = 10 μm. |
PMC1175070_F1_2457.jpg | Describe the main subject of this image. | Tumor cell diapedesis through human microvascular endothelial cell (HMVEC) monolayers. HBL100 cells were pre-labeled with dioctadecyl-3, 3,3', 3'-tetramethylindocarbocyanine percholate (DiI; red), co-cultured with HMVEC monolayers for various time periods, fixed with paraformaldehyde and labeled for F-actin (green). Optical sections obtained by laser scanning confocal microscopy at the focal levels indicated in (a,e,i) identified three major stages of diapedesis: round on top of (a-d), migrating through (e-h), or located underneath (i-l) the endothelium. (b) Cells round on top had filopodial extensions present on the apical surface of a cell (arrows). (g) Thick bundles of F-actin were present at the interface between endothelial cells and the migrating tumor cell (arrow). (h) Migrating tumor cells with portions spread underneath the endothelium (arrows) and (i) those that completed diapedesis often contained prominent stress fibers underneath the endothelium (arrows). No major morphological differences between wild-type and connexin43 (Cx43) expressing HBL100 cells were observed. Bar = 10 μm. |
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