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PMC1112608_F6_2189.jpg | Can you identify the primary element in this image? | PAS staining: (a) foci of clear hepatocytes (arrow) close to the border between the non tumoral PAS positive zone, on the left side, and the PAS negative nodule on the right side of the photograph; (b) a clear focus in the nodule. |
PMC1112617_F1_2190.jpg | Describe the main subject of this image. | LMP1 immunostaining on tissue sections of NPC samples. A. Intense and diffuse LMP1 expression in an NPC biopsy from a 47 year old patient (score 12, 400X) B. Intense LMP1 expression in a limited area in an NPC biopsy from a 17 year old patient (score 7, 600X) C. Moderate and diffuse LMP1 expression in an NPC biopsy from a 44 year patient (score 8, 400X) D. Absence of LMP1 expression in a lung carcinoma biopsy (score 0, 600 X) |
PMC1112617_F1_2193.jpg | What is the dominant medical problem in this image? | LMP1 immunostaining on tissue sections of NPC samples. A. Intense and diffuse LMP1 expression in an NPC biopsy from a 47 year old patient (score 12, 400X) B. Intense LMP1 expression in a limited area in an NPC biopsy from a 17 year old patient (score 7, 600X) C. Moderate and diffuse LMP1 expression in an NPC biopsy from a 44 year patient (score 8, 400X) D. Absence of LMP1 expression in a lung carcinoma biopsy (score 0, 600 X) |
PMC1131892_F4_2194.jpg | What key item or scene is captured in this photo? | AtRaptor1B-/- plants grow slowly. (A), (B) Col and B- plants at 15 days after germination on soil. (C) B- plants bolt later than Col or A-. Shown are shoots from plants 1 month after germination. (D) Growth curve of Col, A- and B- plants. The X-axis represents time after production of the first leaf. The Y-axis represents the number of rosette leaves up to 11; presence of a floral bud is 12; number of cauline leaves plus 12 is 13–16, and values above 16 are the number of shoot apices harboring flowers plus 15. B- plants show slower leaf initiation, later bolting (though at a similar rosette leaf number as Col and A-) and later flowering. |
PMC1131892_F4_2195.jpg | What is the focal point of this photograph? | AtRaptor1B-/- plants grow slowly. (A), (B) Col and B- plants at 15 days after germination on soil. (C) B- plants bolt later than Col or A-. Shown are shoots from plants 1 month after germination. (D) Growth curve of Col, A- and B- plants. The X-axis represents time after production of the first leaf. The Y-axis represents the number of rosette leaves up to 11; presence of a floral bud is 12; number of cauline leaves plus 12 is 13–16, and values above 16 are the number of shoot apices harboring flowers plus 15. B- plants show slower leaf initiation, later bolting (though at a similar rosette leaf number as Col and A-) and later flowering. |
PMC1131892_F4_2197.jpg | Can you identify the primary element in this image? | AtRaptor1B-/- plants grow slowly. (A), (B) Col and B- plants at 15 days after germination on soil. (C) B- plants bolt later than Col or A-. Shown are shoots from plants 1 month after germination. (D) Growth curve of Col, A- and B- plants. The X-axis represents time after production of the first leaf. The Y-axis represents the number of rosette leaves up to 11; presence of a floral bud is 12; number of cauline leaves plus 12 is 13–16, and values above 16 are the number of shoot apices harboring flowers plus 15. B- plants show slower leaf initiation, later bolting (though at a similar rosette leaf number as Col and A-) and later flowering. |
PMC1131895_F2_2202.jpg | What's the most prominent thing you notice in this picture? | Fluorescence lifetime imaging of GFP-PKC expressed in CHO cells. GFP-PKC was transiently expressed in CHO cells as described in the legend in Figure 1 and detailed under Methods. Images were acquired using a Nikon 2000 inverted epifluorescence microscope with a 60× objective and GFP/DsRed filter sets and an ICAM camera. The 2P-FLIM images were collected using the TCSPC fast scanning imaging mode as described under Methods. (a) Conventional epifluorescence image of GFP-PKC distribution in a resting CHO cell, (b) lifetime image of the same cell with the analysis area enclosed by the red line (cytosol) shown (with colour coding) in the inset and giving an average lifetime of ~2.2 ns and (c) with the analysis area enclosed by the red line (nucleus) shown in the inset giving an average lifetime of ~2.0 ns. Cells shown are representative images from replicate experiments. |
PMC1131895_F2_2201.jpg | Describe the main subject of this image. | Fluorescence lifetime imaging of GFP-PKC expressed in CHO cells. GFP-PKC was transiently expressed in CHO cells as described in the legend in Figure 1 and detailed under Methods. Images were acquired using a Nikon 2000 inverted epifluorescence microscope with a 60× objective and GFP/DsRed filter sets and an ICAM camera. The 2P-FLIM images were collected using the TCSPC fast scanning imaging mode as described under Methods. (a) Conventional epifluorescence image of GFP-PKC distribution in a resting CHO cell, (b) lifetime image of the same cell with the analysis area enclosed by the red line (cytosol) shown (with colour coding) in the inset and giving an average lifetime of ~2.2 ns and (c) with the analysis area enclosed by the red line (nucleus) shown in the inset giving an average lifetime of ~2.0 ns. Cells shown are representative images from replicate experiments. |
PMC1131895_F2_2200.jpg | What is the main focus of this visual representation? | Fluorescence lifetime imaging of GFP-PKC expressed in CHO cells. GFP-PKC was transiently expressed in CHO cells as described in the legend in Figure 1 and detailed under Methods. Images were acquired using a Nikon 2000 inverted epifluorescence microscope with a 60× objective and GFP/DsRed filter sets and an ICAM camera. The 2P-FLIM images were collected using the TCSPC fast scanning imaging mode as described under Methods. (a) Conventional epifluorescence image of GFP-PKC distribution in a resting CHO cell, (b) lifetime image of the same cell with the analysis area enclosed by the red line (cytosol) shown (with colour coding) in the inset and giving an average lifetime of ~2.2 ns and (c) with the analysis area enclosed by the red line (nucleus) shown in the inset giving an average lifetime of ~2.0 ns. Cells shown are representative images from replicate experiments. |
PMC1131917_F1_2205.jpg | What key item or scene is captured in this photo? | Plain radiographs of the right elbow. A. AP view, demonstrating no changes. B. Lateral view demonstrating a joint effusion (arrowed). |
PMC1131917_F2_2203.jpg | What stands out most in this visual? | Ultrasound examination of the right elbow. This confirms the presence of a hypoechoic joint effusion (arrowed). |
PMC1131917_F2_2204.jpg | What can you see in this picture? | Ultrasound examination of the right elbow. This confirms the presence of a hypoechoic joint effusion (arrowed). |
PMC1131917_F7_2210.jpg | What is being portrayed in this visual content? | Multiplanar MR imaging of the right elbow. A. T1 weighted axial image at the level of the elbow joint, demonstrating a complex effusion (arrow), particularly adjacent to the proximal ulna. B. Contrast enhanced axial image showing enhancement around the joint (arrow). C. T2 weighted axial image again showing extensive joint effusion D. Coronal contrast enhanced image showing the complex effusion and abnormal signal intensity in the medullary canal of the proximal ulna (arrow). |
PMC1131917_F7_2211.jpg | What object or scene is depicted here? | Multiplanar MR imaging of the right elbow. A. T1 weighted axial image at the level of the elbow joint, demonstrating a complex effusion (arrow), particularly adjacent to the proximal ulna. B. Contrast enhanced axial image showing enhancement around the joint (arrow). C. T2 weighted axial image again showing extensive joint effusion D. Coronal contrast enhanced image showing the complex effusion and abnormal signal intensity in the medullary canal of the proximal ulna (arrow). |
PMC1131917_F7_2212.jpg | What can you see in this picture? | Multiplanar MR imaging of the right elbow. A. T1 weighted axial image at the level of the elbow joint, demonstrating a complex effusion (arrow), particularly adjacent to the proximal ulna. B. Contrast enhanced axial image showing enhancement around the joint (arrow). C. T2 weighted axial image again showing extensive joint effusion D. Coronal contrast enhanced image showing the complex effusion and abnormal signal intensity in the medullary canal of the proximal ulna (arrow). |
PMC1131917_F7_2213.jpg | Can you identify the primary element in this image? | Multiplanar MR imaging of the right elbow. A. T1 weighted axial image at the level of the elbow joint, demonstrating a complex effusion (arrow), particularly adjacent to the proximal ulna. B. Contrast enhanced axial image showing enhancement around the joint (arrow). C. T2 weighted axial image again showing extensive joint effusion D. Coronal contrast enhanced image showing the complex effusion and abnormal signal intensity in the medullary canal of the proximal ulna (arrow). |
PMC1131917_F9_2209.jpg | What is the main focus of this visual representation? | Multiplanar chest CT. This demonstrates mediastinal lymphadenopathy and subtle pulmonary involvement. A. Contrast enhanced axial slice, showing enlarged lymph nodes (arrowed) in the para-aortic window. B. Axial slice showing subcarinal lymphadenopathy (arrowed). C. Coronal slice, showing small nodules in the left upper lobe (arrowed). |
PMC1131917_F9_2207.jpg | What can you see in this picture? | Multiplanar chest CT. This demonstrates mediastinal lymphadenopathy and subtle pulmonary involvement. A. Contrast enhanced axial slice, showing enlarged lymph nodes (arrowed) in the para-aortic window. B. Axial slice showing subcarinal lymphadenopathy (arrowed). C. Coronal slice, showing small nodules in the left upper lobe (arrowed). |
PMC1131917_F9_2208.jpg | What key item or scene is captured in this photo? | Multiplanar chest CT. This demonstrates mediastinal lymphadenopathy and subtle pulmonary involvement. A. Contrast enhanced axial slice, showing enlarged lymph nodes (arrowed) in the para-aortic window. B. Axial slice showing subcarinal lymphadenopathy (arrowed). C. Coronal slice, showing small nodules in the left upper lobe (arrowed). |
PMC1131917_F11_2214.jpg | What is being portrayed in this visual content? | CT guided biopsy of the para-aortic lymph nodes. A. 14 gauge core needle biopsy. B. The core of tissue obtained. C. Histological examination after haematoxylin and eosin staining, demonstrating classic caseating granulomata of tuberculous infection (arrowed). |
PMC1131917_F11_2215.jpg | What can you see in this picture? | CT guided biopsy of the para-aortic lymph nodes. A. 14 gauge core needle biopsy. B. The core of tissue obtained. C. Histological examination after haematoxylin and eosin staining, demonstrating classic caseating granulomata of tuberculous infection (arrowed). |
PMC1131924_F3_2218.jpg | What is the central feature of this picture? | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131924_F3_2222.jpg | Can you identify the primary element in this image? | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131924_F3_2217.jpg | Can you identify the primary element in this image? | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131924_F3_2219.jpg | What stands out most in this visual? | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131924_F3_2220.jpg | Describe the main subject of this image. | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131924_F3_2221.jpg | Can you identify the primary element in this image? | Five-day OSE culture maintained in the DMEM-HG medium with phenol red as in Fig. 2. a) Cell of oocyte phenotype with two nuclei. The centrally located nucleus shows ZP immunostaining (arrowhead) but adjacent nucleus is unstained (arrow). Note ZP+ intermediate filaments. b) The same cell subjected to double color (ZP/CK5,6,8,17) immunohistochemistry shows no additional (dark blue) staining. c) Another large cell stained for vimentin shows expression in intermediate filaments and two unstained large nuclei (arrows). d) Double color CK18/ZP staining shows intermediate filaments and ZP+ centrally located nucleus (arrowhead) and unstained adjacent structure resembling the polar body (arrow). e) Cell with centrally located ZP+ nucleus (arrowhead) and unstained fragmented adjacent structure (arrow). Note a lack of ZP+ intermediate filaments (compare with panel a) and surface ZP expression (solid arrow; compare with black arrow, panel d). f) Nuclear expression (arrowhead) of meiotically expressed PS1 carbohydrate ZP antigen. Note focally enhanced (coarse) staining (arrow) in one of the two rounded structures. g) Two cells showing a lack of nuclear PS1 expression (arrowheads), but strong staining is associated with adjacent polar-like bodies (arrows). |
PMC1131928_F2_2230.jpg | What is the dominant medical problem in this image? | Confocal images of rat lung slice co-stained with either the LIVE® or DEAD® viability stain and the specific alveolar type I cell antibody, VIIIB2. A) A 200 μm rat lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 532 secondary antibody and excited at 488 nm (green emission) to show living cells. B) The same lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 546 secondary antibody and excited at 532 nm (red emission) to show the Alexa Fluor 546 secondary antibody staining at alveolar type I cells. C) Overlay of images (A) and (B) showing that VIIIB2 immunoreactivity is clearly restricted to elongated, thin cells located at the edge of the alveolar space (indicated by the arrows), characteristic of ATI cells. D) A 200 μm rat lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 488 nm (green emission) to show secondary antibody staining of alveolar type I cells. E) The same lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 532 nm (red emission) to show the EthD-1 positive, dead cell nuclei. F) Overlay of images (D) and (E) showing that VIIIB2 immunoreactivity and the dead cell stain do not co-localise. G) Graph showing quantification of dead cells per field (105 μM × 105 μM) following treatments indicated at the bottom of each data set. |
PMC1131928_F2_2226.jpg | What can you see in this picture? | Confocal images of rat lung slice co-stained with either the LIVE® or DEAD® viability stain and the specific alveolar type I cell antibody, VIIIB2. A) A 200 μm rat lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 532 secondary antibody and excited at 488 nm (green emission) to show living cells. B) The same lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 546 secondary antibody and excited at 532 nm (red emission) to show the Alexa Fluor 546 secondary antibody staining at alveolar type I cells. C) Overlay of images (A) and (B) showing that VIIIB2 immunoreactivity is clearly restricted to elongated, thin cells located at the edge of the alveolar space (indicated by the arrows), characteristic of ATI cells. D) A 200 μm rat lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 488 nm (green emission) to show secondary antibody staining of alveolar type I cells. E) The same lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 532 nm (red emission) to show the EthD-1 positive, dead cell nuclei. F) Overlay of images (D) and (E) showing that VIIIB2 immunoreactivity and the dead cell stain do not co-localise. G) Graph showing quantification of dead cells per field (105 μM × 105 μM) following treatments indicated at the bottom of each data set. |
PMC1131928_F2_2227.jpg | What is the core subject represented in this visual? | Confocal images of rat lung slice co-stained with either the LIVE® or DEAD® viability stain and the specific alveolar type I cell antibody, VIIIB2. A) A 200 μm rat lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 532 secondary antibody and excited at 488 nm (green emission) to show living cells. B) The same lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 546 secondary antibody and excited at 532 nm (red emission) to show the Alexa Fluor 546 secondary antibody staining at alveolar type I cells. C) Overlay of images (A) and (B) showing that VIIIB2 immunoreactivity is clearly restricted to elongated, thin cells located at the edge of the alveolar space (indicated by the arrows), characteristic of ATI cells. D) A 200 μm rat lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 488 nm (green emission) to show secondary antibody staining of alveolar type I cells. E) The same lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 532 nm (red emission) to show the EthD-1 positive, dead cell nuclei. F) Overlay of images (D) and (E) showing that VIIIB2 immunoreactivity and the dead cell stain do not co-localise. G) Graph showing quantification of dead cells per field (105 μM × 105 μM) following treatments indicated at the bottom of each data set. |
PMC1131928_F2_2225.jpg | What is the dominant medical problem in this image? | Confocal images of rat lung slice co-stained with either the LIVE® or DEAD® viability stain and the specific alveolar type I cell antibody, VIIIB2. A) A 200 μm rat lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 532 secondary antibody and excited at 488 nm (green emission) to show living cells. B) The same lung slice incubated with the live stain (1 μM Calcein-AM) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 546 secondary antibody and excited at 532 nm (red emission) to show the Alexa Fluor 546 secondary antibody staining at alveolar type I cells. C) Overlay of images (A) and (B) showing that VIIIB2 immunoreactivity is clearly restricted to elongated, thin cells located at the edge of the alveolar space (indicated by the arrows), characteristic of ATI cells. D) A 200 μm rat lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical staining with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 488 nm (green emission) to show secondary antibody staining of alveolar type I cells. E) The same lung slice incubated with the dead stain (4 μM EthD-1) following live immunohistochemical treatment with VIIIB2 primary antibody/Alexa Fluor 488 secondary antibody and excited at 532 nm (red emission) to show the EthD-1 positive, dead cell nuclei. F) Overlay of images (D) and (E) showing that VIIIB2 immunoreactivity and the dead cell stain do not co-localise. G) Graph showing quantification of dead cells per field (105 μM × 105 μM) following treatments indicated at the bottom of each data set. |
PMC1131931_F1_2232.jpg | What is the core subject represented in this visual? | Transmission electron microscopy of AAV2 and Ad5 particles in human cells. (A) AAV2 and Ad5 particles in the nucleus of a HeLa cell at 48 hours after co-infection. Magnification: × 15,000. (B) AAV2 virions in a HeLa cell at 48 hours after co-infection with Ad5. Magnification: × 40,000. |
PMC1131931_F1_2233.jpg | What is the core subject represented in this visual? | Transmission electron microscopy of AAV2 and Ad5 particles in human cells. (A) AAV2 and Ad5 particles in the nucleus of a HeLa cell at 48 hours after co-infection. Magnification: × 15,000. (B) AAV2 virions in a HeLa cell at 48 hours after co-infection with Ad5. Magnification: × 40,000. |
PMC1135295_pbio-0030192-g002_2234.jpg | Describe the main subject of this image. | Distribution of GFP-Expressing Endothelia and Kupffer Cells in the Liver(A) Confocal microscopy demonstrates the GFP distribution in sinusoidal endothelia from a Tie2-GFP mouse. GFP is most prominent in the perinuclear region (arrowheads) of endothelia located in the periphery of the liver lobule.(B) A still image from an intravital movie shows GFP-expressing endothelia lining the sinusoids of a Tie2-GFP mouse. Kupffer cells can be identified by their orange autofluorescent lysosomes (arrowheads).(C) Star-shaped Kupffer cells (arrowheads) are located in sinusoids of a lys-EGFP-ki mouse liver.(D) Round blood granulocytes (arrows), traveling with the bloodstream or crawling along the sinusoidal cell layer, exhibit a stronger GFP signal than Kupffer cells (still image extracted from an intravital movie). Note the orange autofluorescence of the Kupffer cell lysosomes (arrowheads).Bar = 10 μm. See Videos S1 and S2. |
PMC1135295_pbio-0030192-g002_2235.jpg | What is the principal component of this image? | Distribution of GFP-Expressing Endothelia and Kupffer Cells in the Liver(A) Confocal microscopy demonstrates the GFP distribution in sinusoidal endothelia from a Tie2-GFP mouse. GFP is most prominent in the perinuclear region (arrowheads) of endothelia located in the periphery of the liver lobule.(B) A still image from an intravital movie shows GFP-expressing endothelia lining the sinusoids of a Tie2-GFP mouse. Kupffer cells can be identified by their orange autofluorescent lysosomes (arrowheads).(C) Star-shaped Kupffer cells (arrowheads) are located in sinusoids of a lys-EGFP-ki mouse liver.(D) Round blood granulocytes (arrows), traveling with the bloodstream or crawling along the sinusoidal cell layer, exhibit a stronger GFP signal than Kupffer cells (still image extracted from an intravital movie). Note the orange autofluorescence of the Kupffer cell lysosomes (arrowheads).Bar = 10 μm. See Videos S1 and S2. |
PMC1135298_pbio-0030201-g004_2247.jpg | What is the principal component of this image? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2246.jpg | What key item or scene is captured in this photo? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2239.jpg | What is the dominant medical problem in this image? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2242.jpg | What object or scene is depicted here? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2243.jpg | What is the principal component of this image? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2240.jpg | What does this image primarily show? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2238.jpg | What is the dominant medical problem in this image? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2241.jpg | What is the main focus of this visual representation? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1135298_pbio-0030201-g004_2244.jpg | What does this image primarily show? | Hemocyte Abnormalities in adgf-a Mutant Larvae(A–E) Differential interference contrast microscopy of living circulating hemocytes (magnification 200×; scale bar, 10 μm). Round, nonadhesive plasmatocytes from wild-type larva (A). Hemocytes from the adgf-a mutant developing filamentous extensions (B and C) or membranous extension surrounding the cell (D). Large flat lamellocyte from the adgf-a mutant (E).(F and G) Differential interference contrast and fluorescent microscopy (merged image) of living circulating hemocytes stained by the Hml-GFP marker (magnification 100×; scale bar, 10 μm). While most of the cells from wild-type larvae are GFP-positive (F), just few of the cells from late third instar adgf-a larvae are stained by GFP at this stage (G).(H–J) Fluorescence microscopy of living larvae with Hml-GFP stained hemocytes (magnification 40×; scale bar, 100 μm). Posterior part of late third-instar wild-type larva (H). Middle sections of early third-instar larvae of wild type (I) and adgf-a mutant (J). |
PMC1140680_pbio-0030223-g002_2248.jpg | What is being portrayed in this visual content? | Survivorship (lx) Analysis of Life Span of Female Drosophila on Different Food RegimesColour/Symbol of the curves shows yeast level while the line type represents sugar levels in the respective foods. (A) and (B) are independent repeats. In both cases, changing caloric content of the food by altering yeast levels had a much greater effect on life span than that seen when the same change in caloric content was brought about by manipulating sugar levels. |
PMC1141267_pbio-0030236-g010_2254.jpg | What object or scene is depicted here? | loqsf00791 Fail to Maintain Germ-Line Stem Cells(A) Wild-type ovarioles contain a germarium and a developmentally ordered array of six to eight egg chambers, whereas loqs
f00791 mutant ovarioles contain a smaller than normal germarium, two or three pre-vitellogenic egg chambers, and a late-stage egg chamber. Wild-type and loqs ovarioles are shown at the same magnification.(B) In wild-type ovarioles, the germarium contains several newly formed germ-line cysts surrounded by somatic follicle cells. In contrast, loqs
f00791 mutant germaria contain few germ-line cells, which are not organized into distinct cysts. The follicle cell layer is also significantly reduced inloqs
f00791 germaria.(C) Wild-type and loqs mutant germaria labeled for α-Spectrin (green) and filamentous Actin (red). In wild type, anti-α-Spectrin labels the spectrosome (ss), a structure characteristic of germ-line stem cells, which are normally found at the anterior of the germarium, apposed to the somatic terminal cells (tc). The cystoblasts, the daughters of the stem cells, also contain a spectrosome, but are located posterior to the stem cells. In loqs mutant ovaries, spectrosome-containing cells were not detected, indicating that normal germ-line stem cells are not present. These observations indicate that stem cells are not maintained.In (A) and (B), ovaries were labeled for filamentous actin (red) using rhodamine phalloidin, DNA (blue) using TOTO3 (Molecular Probes), and the germ-line marker Vasa (green) using rabbit anti-Vasa antibody detected with fluorescein-conjugated anti-rabbit secondary antibody. In (B) and (C), wild-type and loqs germaria are shown at the same magnification. |
PMC1141267_pbio-0030236-g010_2250.jpg | What is the core subject represented in this visual? | loqsf00791 Fail to Maintain Germ-Line Stem Cells(A) Wild-type ovarioles contain a germarium and a developmentally ordered array of six to eight egg chambers, whereas loqs
f00791 mutant ovarioles contain a smaller than normal germarium, two or three pre-vitellogenic egg chambers, and a late-stage egg chamber. Wild-type and loqs ovarioles are shown at the same magnification.(B) In wild-type ovarioles, the germarium contains several newly formed germ-line cysts surrounded by somatic follicle cells. In contrast, loqs
f00791 mutant germaria contain few germ-line cells, which are not organized into distinct cysts. The follicle cell layer is also significantly reduced inloqs
f00791 germaria.(C) Wild-type and loqs mutant germaria labeled for α-Spectrin (green) and filamentous Actin (red). In wild type, anti-α-Spectrin labels the spectrosome (ss), a structure characteristic of germ-line stem cells, which are normally found at the anterior of the germarium, apposed to the somatic terminal cells (tc). The cystoblasts, the daughters of the stem cells, also contain a spectrosome, but are located posterior to the stem cells. In loqs mutant ovaries, spectrosome-containing cells were not detected, indicating that normal germ-line stem cells are not present. These observations indicate that stem cells are not maintained.In (A) and (B), ovaries were labeled for filamentous actin (red) using rhodamine phalloidin, DNA (blue) using TOTO3 (Molecular Probes), and the germ-line marker Vasa (green) using rabbit anti-Vasa antibody detected with fluorescein-conjugated anti-rabbit secondary antibody. In (B) and (C), wild-type and loqs germaria are shown at the same magnification. |
PMC1141267_pbio-0030236-g010_2252.jpg | What stands out most in this visual? | loqsf00791 Fail to Maintain Germ-Line Stem Cells(A) Wild-type ovarioles contain a germarium and a developmentally ordered array of six to eight egg chambers, whereas loqs
f00791 mutant ovarioles contain a smaller than normal germarium, two or three pre-vitellogenic egg chambers, and a late-stage egg chamber. Wild-type and loqs ovarioles are shown at the same magnification.(B) In wild-type ovarioles, the germarium contains several newly formed germ-line cysts surrounded by somatic follicle cells. In contrast, loqs
f00791 mutant germaria contain few germ-line cells, which are not organized into distinct cysts. The follicle cell layer is also significantly reduced inloqs
f00791 germaria.(C) Wild-type and loqs mutant germaria labeled for α-Spectrin (green) and filamentous Actin (red). In wild type, anti-α-Spectrin labels the spectrosome (ss), a structure characteristic of germ-line stem cells, which are normally found at the anterior of the germarium, apposed to the somatic terminal cells (tc). The cystoblasts, the daughters of the stem cells, also contain a spectrosome, but are located posterior to the stem cells. In loqs mutant ovaries, spectrosome-containing cells were not detected, indicating that normal germ-line stem cells are not present. These observations indicate that stem cells are not maintained.In (A) and (B), ovaries were labeled for filamentous actin (red) using rhodamine phalloidin, DNA (blue) using TOTO3 (Molecular Probes), and the germ-line marker Vasa (green) using rabbit anti-Vasa antibody detected with fluorescein-conjugated anti-rabbit secondary antibody. In (B) and (C), wild-type and loqs germaria are shown at the same magnification. |
PMC1141267_pbio-0030236-g010_2253.jpg | What is the focal point of this photograph? | loqsf00791 Fail to Maintain Germ-Line Stem Cells(A) Wild-type ovarioles contain a germarium and a developmentally ordered array of six to eight egg chambers, whereas loqs
f00791 mutant ovarioles contain a smaller than normal germarium, two or three pre-vitellogenic egg chambers, and a late-stage egg chamber. Wild-type and loqs ovarioles are shown at the same magnification.(B) In wild-type ovarioles, the germarium contains several newly formed germ-line cysts surrounded by somatic follicle cells. In contrast, loqs
f00791 mutant germaria contain few germ-line cells, which are not organized into distinct cysts. The follicle cell layer is also significantly reduced inloqs
f00791 germaria.(C) Wild-type and loqs mutant germaria labeled for α-Spectrin (green) and filamentous Actin (red). In wild type, anti-α-Spectrin labels the spectrosome (ss), a structure characteristic of germ-line stem cells, which are normally found at the anterior of the germarium, apposed to the somatic terminal cells (tc). The cystoblasts, the daughters of the stem cells, also contain a spectrosome, but are located posterior to the stem cells. In loqs mutant ovaries, spectrosome-containing cells were not detected, indicating that normal germ-line stem cells are not present. These observations indicate that stem cells are not maintained.In (A) and (B), ovaries were labeled for filamentous actin (red) using rhodamine phalloidin, DNA (blue) using TOTO3 (Molecular Probes), and the germ-line marker Vasa (green) using rabbit anti-Vasa antibody detected with fluorescein-conjugated anti-rabbit secondary antibody. In (B) and (C), wild-type and loqs germaria are shown at the same magnification. |
PMC1141267_pbio-0030236-g010_2251.jpg | Can you identify the primary element in this image? | loqsf00791 Fail to Maintain Germ-Line Stem Cells(A) Wild-type ovarioles contain a germarium and a developmentally ordered array of six to eight egg chambers, whereas loqs
f00791 mutant ovarioles contain a smaller than normal germarium, two or three pre-vitellogenic egg chambers, and a late-stage egg chamber. Wild-type and loqs ovarioles are shown at the same magnification.(B) In wild-type ovarioles, the germarium contains several newly formed germ-line cysts surrounded by somatic follicle cells. In contrast, loqs
f00791 mutant germaria contain few germ-line cells, which are not organized into distinct cysts. The follicle cell layer is also significantly reduced inloqs
f00791 germaria.(C) Wild-type and loqs mutant germaria labeled for α-Spectrin (green) and filamentous Actin (red). In wild type, anti-α-Spectrin labels the spectrosome (ss), a structure characteristic of germ-line stem cells, which are normally found at the anterior of the germarium, apposed to the somatic terminal cells (tc). The cystoblasts, the daughters of the stem cells, also contain a spectrosome, but are located posterior to the stem cells. In loqs mutant ovaries, spectrosome-containing cells were not detected, indicating that normal germ-line stem cells are not present. These observations indicate that stem cells are not maintained.In (A) and (B), ovaries were labeled for filamentous actin (red) using rhodamine phalloidin, DNA (blue) using TOTO3 (Molecular Probes), and the germ-line marker Vasa (green) using rabbit anti-Vasa antibody detected with fluorescein-conjugated anti-rabbit secondary antibody. In (B) and (C), wild-type and loqs germaria are shown at the same magnification. |
PMC1142305_F7_2257.jpg | What is the central feature of this picture? | SEM observation of retained liposomes on G-25 fine beads. A : Sephadex beads non incubated with liposomes, fixed with glutaraldehyde, washed, stained and prepared for SEM. B, C, D: Liposomes, containing PC and PE (6/4), retained by a 2 mL sephadex G-25 fine columns were fixed by glutaraldehyde, washed, stained and prepared for SEM observations. |
PMC1142305_F7_2259.jpg | What is the central feature of this picture? | SEM observation of retained liposomes on G-25 fine beads. A : Sephadex beads non incubated with liposomes, fixed with glutaraldehyde, washed, stained and prepared for SEM. B, C, D: Liposomes, containing PC and PE (6/4), retained by a 2 mL sephadex G-25 fine columns were fixed by glutaraldehyde, washed, stained and prepared for SEM observations. |
PMC1142305_F7_2258.jpg | What does this image primarily show? | SEM observation of retained liposomes on G-25 fine beads. A : Sephadex beads non incubated with liposomes, fixed with glutaraldehyde, washed, stained and prepared for SEM. B, C, D: Liposomes, containing PC and PE (6/4), retained by a 2 mL sephadex G-25 fine columns were fixed by glutaraldehyde, washed, stained and prepared for SEM observations. |
PMC1142305_F7_2260.jpg | Can you identify the primary element in this image? | SEM observation of retained liposomes on G-25 fine beads. A : Sephadex beads non incubated with liposomes, fixed with glutaraldehyde, washed, stained and prepared for SEM. B, C, D: Liposomes, containing PC and PE (6/4), retained by a 2 mL sephadex G-25 fine columns were fixed by glutaraldehyde, washed, stained and prepared for SEM observations. |
PMC1142324_F3_2265.jpg | What stands out most in this visual? | Neuronal ADAM22 mRNA expression in the CNS. To determine the ADAM22 mRNA distribution, in situ hybridisation analysis using 35S-labeled probe was performed. Coronal (A, B) and sagittal (C, D) sections of the mouse brain and spinal cord (E) were shown. Using the antisense probe (A, C), strong signals were obtained, especially in the hippocampus and the cerebellum, while no signals was detected by the sense probe (B, D). In the spinal cord, autoradiograms of ADAM22 mRNA was detected in the grey matter (E). |
PMC1142324_F3_2261.jpg | What object or scene is depicted here? | Neuronal ADAM22 mRNA expression in the CNS. To determine the ADAM22 mRNA distribution, in situ hybridisation analysis using 35S-labeled probe was performed. Coronal (A, B) and sagittal (C, D) sections of the mouse brain and spinal cord (E) were shown. Using the antisense probe (A, C), strong signals were obtained, especially in the hippocampus and the cerebellum, while no signals was detected by the sense probe (B, D). In the spinal cord, autoradiograms of ADAM22 mRNA was detected in the grey matter (E). |
PMC1142324_F3_2262.jpg | What can you see in this picture? | Neuronal ADAM22 mRNA expression in the CNS. To determine the ADAM22 mRNA distribution, in situ hybridisation analysis using 35S-labeled probe was performed. Coronal (A, B) and sagittal (C, D) sections of the mouse brain and spinal cord (E) were shown. Using the antisense probe (A, C), strong signals were obtained, especially in the hippocampus and the cerebellum, while no signals was detected by the sense probe (B, D). In the spinal cord, autoradiograms of ADAM22 mRNA was detected in the grey matter (E). |
PMC1142324_F3_2263.jpg | What is the focal point of this photograph? | Neuronal ADAM22 mRNA expression in the CNS. To determine the ADAM22 mRNA distribution, in situ hybridisation analysis using 35S-labeled probe was performed. Coronal (A, B) and sagittal (C, D) sections of the mouse brain and spinal cord (E) were shown. Using the antisense probe (A, C), strong signals were obtained, especially in the hippocampus and the cerebellum, while no signals was detected by the sense probe (B, D). In the spinal cord, autoradiograms of ADAM22 mRNA was detected in the grey matter (E). |
PMC1142324_F3_2264.jpg | Describe the main subject of this image. | Neuronal ADAM22 mRNA expression in the CNS. To determine the ADAM22 mRNA distribution, in situ hybridisation analysis using 35S-labeled probe was performed. Coronal (A, B) and sagittal (C, D) sections of the mouse brain and spinal cord (E) were shown. Using the antisense probe (A, C), strong signals were obtained, especially in the hippocampus and the cerebellum, while no signals was detected by the sense probe (B, D). In the spinal cord, autoradiograms of ADAM22 mRNA was detected in the grey matter (E). |
PMC1142324_F6_2267.jpg | What object or scene is depicted here? | Electron microscopic analysis of sciatic nerves. Electron micrographs of the sciatic nerves from Adam22 +/- (A) and Adam22 -/- (B) mice at postnatal day 10 are shown. In the heterozygote (A), thick myelin was formed, while no myelin was formed in the ADAM22-deficient mouse (B). The axons looked normal in each genotype. |
PMC1142324_F6_2266.jpg | What is the principal component of this image? | Electron microscopic analysis of sciatic nerves. Electron micrographs of the sciatic nerves from Adam22 +/- (A) and Adam22 -/- (B) mice at postnatal day 10 are shown. In the heterozygote (A), thick myelin was formed, while no myelin was formed in the ADAM22-deficient mouse (B). The axons looked normal in each genotype. |
PMC1142325_F10_2269.jpg | What is the central feature of this picture? | Detection of NSE (green) and GFAP (red) by immunocytochemistry in neurons and glia. Cells were labelled with either anti-NSE or anti-GFAP and visualized by fluorescence microscopy. Neuronal cells were grown in Neurobasal medium supplemented with 2% B27 and maintained for 7 days in culture. Glial cells were grown in DMEM supplemented with 10% Fetal Bovine Serum and maintained for 7 days in culture. (A) Neurons labelled with anti-NSE. (B) Glial cells labelled with anti-GFAP. Images were taken at 300× magnification. |
PMC1142325_F10_2268.jpg | What's the most prominent thing you notice in this picture? | Detection of NSE (green) and GFAP (red) by immunocytochemistry in neurons and glia. Cells were labelled with either anti-NSE or anti-GFAP and visualized by fluorescence microscopy. Neuronal cells were grown in Neurobasal medium supplemented with 2% B27 and maintained for 7 days in culture. Glial cells were grown in DMEM supplemented with 10% Fetal Bovine Serum and maintained for 7 days in culture. (A) Neurons labelled with anti-NSE. (B) Glial cells labelled with anti-GFAP. Images were taken at 300× magnification. |
PMC1142332_F3_2274.jpg | What is being portrayed in this visual content? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142332_F3_2270.jpg | What's the most prominent thing you notice in this picture? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142332_F3_2273.jpg | Can you identify the primary element in this image? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142332_F3_2277.jpg | What's the most prominent thing you notice in this picture? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142332_F3_2271.jpg | What does this image primarily show? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142332_F3_2272.jpg | What object or scene is depicted here? | Scanning electron micrograph of NG97 cell line. A, B: small rounded cells presenting blebs (Bl) and filopodia (Fi) on their surfaces; C, D, E: dendritic-like cells with extensive cytoplasmatic prolongations. The area in the rectangle is shown at higher magnification in F; G: culture with two morphologic distinct cellular types; H: fibroblastic-like cells presenting microvilli (Mi) on the membrane surface. |
PMC1142517_F1_2278.jpg | What key item or scene is captured in this photo? | Results of the cylindrical NEMA phantom study. 2D acquisition image (upper left) and 3D acquisition image (upper right) reconstructed using FBP with applied 6 mm (FWHM) Gaussian filter. Corresponding ACF images of 2D (lower left) and 3D image (lower right). |
PMC1142517_F1_2279.jpg | Describe the main subject of this image. | Results of the cylindrical NEMA phantom study. 2D acquisition image (upper left) and 3D acquisition image (upper right) reconstructed using FBP with applied 6 mm (FWHM) Gaussian filter. Corresponding ACF images of 2D (lower left) and 3D image (lower right). |
PMC1143574_F1_2283.jpg | Can you identify the primary element in this image? | Microscopic appearance of tumor cells and fibroblasts. (a) Numerous clumps of UACC-812 tumor cells resembling abortive mammary glands are evident. (b) UACC-812 breast cancer cells seeded at low density on a monolayer of serum-activated fibroblasts. Note the gland formation and the invasive infiltration by tumor cells into fibroblasts. Primary tumor cells derived from (c) human ductal carcinoma in situ and from (d) invasive ductal carcinoma co-cultured with fibroblasts yielded clonogenic growth next to myofibroblasts that expressed α-smooth muscle actin (pink immunostain). Breast cancer cells grown (e) on a monolayer of B16 cells or (f) without a monolayer of fibroblasts generally grew as individual cells identifiable by their red staining for epithelial membrane antigen. (g) Serum-activated fibroblasts also uniformly expressed abundant syndecan-1, as detected by immunocytochemical staining. |
PMC1143574_F1_2285.jpg | What stands out most in this visual? | Microscopic appearance of tumor cells and fibroblasts. (a) Numerous clumps of UACC-812 tumor cells resembling abortive mammary glands are evident. (b) UACC-812 breast cancer cells seeded at low density on a monolayer of serum-activated fibroblasts. Note the gland formation and the invasive infiltration by tumor cells into fibroblasts. Primary tumor cells derived from (c) human ductal carcinoma in situ and from (d) invasive ductal carcinoma co-cultured with fibroblasts yielded clonogenic growth next to myofibroblasts that expressed α-smooth muscle actin (pink immunostain). Breast cancer cells grown (e) on a monolayer of B16 cells or (f) without a monolayer of fibroblasts generally grew as individual cells identifiable by their red staining for epithelial membrane antigen. (g) Serum-activated fibroblasts also uniformly expressed abundant syndecan-1, as detected by immunocytochemical staining. |
PMC1143777_F1_2289.jpg | What object or scene is depicted here? | SPCH > SIL activations. Areas of greater activation for speech (SPCH) than silence (SIL); voxels thresholded at p < .005 uncorrected. Note the lack of activity in Heschl's gyrus (top), likely the result of stimulation by the acoustic noise of the scanner. |
PMC1143777_F1_2287.jpg | What's the most prominent thing you notice in this picture? | SPCH > SIL activations. Areas of greater activation for speech (SPCH) than silence (SIL); voxels thresholded at p < .005 uncorrected. Note the lack of activity in Heschl's gyrus (top), likely the result of stimulation by the acoustic noise of the scanner. |
PMC1143777_F1_2291.jpg | Can you identify the primary element in this image? | SPCH > SIL activations. Areas of greater activation for speech (SPCH) than silence (SIL); voxels thresholded at p < .005 uncorrected. Note the lack of activity in Heschl's gyrus (top), likely the result of stimulation by the acoustic noise of the scanner. |
PMC1143777_F1_2286.jpg | Describe the main subject of this image. | SPCH > SIL activations. Areas of greater activation for speech (SPCH) than silence (SIL); voxels thresholded at p < .005 uncorrected. Note the lack of activity in Heschl's gyrus (top), likely the result of stimulation by the acoustic noise of the scanner. |
PMC1143777_F1_2290.jpg | Can you identify the primary element in this image? | SPCH > SIL activations. Areas of greater activation for speech (SPCH) than silence (SIL); voxels thresholded at p < .005 uncorrected. Note the lack of activity in Heschl's gyrus (top), likely the result of stimulation by the acoustic noise of the scanner. |
PMC1143777_F3_2294.jpg | Describe the main subject of this image. | Peak activations and time series from individual subjects for the DEV > STD contrast. A. Peak activations for the DEV > STD contrast in each subject nearest to the group mean plotted on a translucent rendering of grey matter. B. Time series for responses to all speech stimuli in each individual's peak., C. Mean percent signal change at scans 2 and 3 (approximately 2–7 seconds after the fourth syllable in each train) for STD and DEV trials. D. Mean time series for DEV and STD for all subjects. |
PMC1151600_pbio-0030233-g003_2299.jpg | What key item or scene is captured in this photo? | Endosomes Labeled with Two Markers Show Presence of Rab7 in Early EndosomesVero cells were fixed and viewed by confocal microscopy.(A) Confocal microscopy of immunolabeled cells using anti-EEA1 (green) and anti-Rab7 (red) antibodies,(B–F) The cells were transfected with fluorescent-protein-labeled constructs as follows: (B) GFP-Rab5 and RFP-Rab7, (C) GFP-Rab4 and RFP-Rab5, (D) Arf1-GFP and RFP-Rab5, (E) GFP-Rab4 and RFP-Rab7, and (F) Arf1-GFP and RFP-Rab7.Arrowheads show individual endosomes positive for one of the two markers, and arrows indicate endosomes positive for two markers. Scale bars represent 10 μm. |
PMC1151600_pbio-0030233-g003_2297.jpg | What is the main focus of this visual representation? | Endosomes Labeled with Two Markers Show Presence of Rab7 in Early EndosomesVero cells were fixed and viewed by confocal microscopy.(A) Confocal microscopy of immunolabeled cells using anti-EEA1 (green) and anti-Rab7 (red) antibodies,(B–F) The cells were transfected with fluorescent-protein-labeled constructs as follows: (B) GFP-Rab5 and RFP-Rab7, (C) GFP-Rab4 and RFP-Rab5, (D) Arf1-GFP and RFP-Rab5, (E) GFP-Rab4 and RFP-Rab7, and (F) Arf1-GFP and RFP-Rab7.Arrowheads show individual endosomes positive for one of the two markers, and arrows indicate endosomes positive for two markers. Scale bars represent 10 μm. |
PMC1151600_pbio-0030233-g003_2302.jpg | What is being portrayed in this visual content? | Endosomes Labeled with Two Markers Show Presence of Rab7 in Early EndosomesVero cells were fixed and viewed by confocal microscopy.(A) Confocal microscopy of immunolabeled cells using anti-EEA1 (green) and anti-Rab7 (red) antibodies,(B–F) The cells were transfected with fluorescent-protein-labeled constructs as follows: (B) GFP-Rab5 and RFP-Rab7, (C) GFP-Rab4 and RFP-Rab5, (D) Arf1-GFP and RFP-Rab5, (E) GFP-Rab4 and RFP-Rab7, and (F) Arf1-GFP and RFP-Rab7.Arrowheads show individual endosomes positive for one of the two markers, and arrows indicate endosomes positive for two markers. Scale bars represent 10 μm. |
PMC1151600_pbio-0030233-g003_2300.jpg | What key item or scene is captured in this photo? | Endosomes Labeled with Two Markers Show Presence of Rab7 in Early EndosomesVero cells were fixed and viewed by confocal microscopy.(A) Confocal microscopy of immunolabeled cells using anti-EEA1 (green) and anti-Rab7 (red) antibodies,(B–F) The cells were transfected with fluorescent-protein-labeled constructs as follows: (B) GFP-Rab5 and RFP-Rab7, (C) GFP-Rab4 and RFP-Rab5, (D) Arf1-GFP and RFP-Rab5, (E) GFP-Rab4 and RFP-Rab7, and (F) Arf1-GFP and RFP-Rab7.Arrowheads show individual endosomes positive for one of the two markers, and arrows indicate endosomes positive for two markers. Scale bars represent 10 μm. |
PMC1156867_F4_2306.jpg | Describe the main subject of this image. | Difference display visualization. Three clusters displayed traditionally on the left and in our difference image visualization on the right. In the difference display, the large top bar on each cluster shows the cluster average, each gene is displayed as its difference from that average (green indicates expressed less than the cluster average, red shows more expressed, and black means equally expressed with the cluster average). Cluster (a) is a coherent cluster of genes and appears very dark because of its homogeneity. Cluster (b) is another dark, uniform cluster, but it also contains one randomly inserted gene, which can be easily identified in our difference display. Cluster (c) contains a random selection of genes, and its randomness is clear from the brightness of the difference display. This difference display allows for quick assessment of overall cluster homogeneity and facilitates quick outlier detection. (Data and clusters a & b from [19]) |
PMC1156867_F4_2304.jpg | Describe the main subject of this image. | Difference display visualization. Three clusters displayed traditionally on the left and in our difference image visualization on the right. In the difference display, the large top bar on each cluster shows the cluster average, each gene is displayed as its difference from that average (green indicates expressed less than the cluster average, red shows more expressed, and black means equally expressed with the cluster average). Cluster (a) is a coherent cluster of genes and appears very dark because of its homogeneity. Cluster (b) is another dark, uniform cluster, but it also contains one randomly inserted gene, which can be easily identified in our difference display. Cluster (c) contains a random selection of genes, and its randomness is clear from the brightness of the difference display. This difference display allows for quick assessment of overall cluster homogeneity and facilitates quick outlier detection. (Data and clusters a & b from [19]) |
PMC1156867_F4_2305.jpg | What stands out most in this visual? | Difference display visualization. Three clusters displayed traditionally on the left and in our difference image visualization on the right. In the difference display, the large top bar on each cluster shows the cluster average, each gene is displayed as its difference from that average (green indicates expressed less than the cluster average, red shows more expressed, and black means equally expressed with the cluster average). Cluster (a) is a coherent cluster of genes and appears very dark because of its homogeneity. Cluster (b) is another dark, uniform cluster, but it also contains one randomly inserted gene, which can be easily identified in our difference display. Cluster (c) contains a random selection of genes, and its randomness is clear from the brightness of the difference display. This difference display allows for quick assessment of overall cluster homogeneity and facilitates quick outlier detection. (Data and clusters a & b from [19]) |
PMC1156867_F4_2303.jpg | What is the core subject represented in this visual? | Difference display visualization. Three clusters displayed traditionally on the left and in our difference image visualization on the right. In the difference display, the large top bar on each cluster shows the cluster average, each gene is displayed as its difference from that average (green indicates expressed less than the cluster average, red shows more expressed, and black means equally expressed with the cluster average). Cluster (a) is a coherent cluster of genes and appears very dark because of its homogeneity. Cluster (b) is another dark, uniform cluster, but it also contains one randomly inserted gene, which can be easily identified in our difference display. Cluster (c) contains a random selection of genes, and its randomness is clear from the brightness of the difference display. This difference display allows for quick assessment of overall cluster homogeneity and facilitates quick outlier detection. (Data and clusters a & b from [19]) |
PMC1156898_F4_2312.jpg | Describe the main subject of this image. | Growth at different temperatures of yeast expressing TRZ1, trz1-537 or trz1-538 alleles on media with various carbon sources added. Yeast bearing TRZ1 alleles integrated at the LEU2 locus [YL13-01 (WT), YL13-02 (tr1-537) and YL13-03 (trz1-538)] were serially diluted and spotted onto SC-LEU with different sugars added. Plates were incubated at RT (upper), 30°C (middle) and 37°C (lower). |
PMC1156898_F4_2309.jpg | What does this image primarily show? | Growth at different temperatures of yeast expressing TRZ1, trz1-537 or trz1-538 alleles on media with various carbon sources added. Yeast bearing TRZ1 alleles integrated at the LEU2 locus [YL13-01 (WT), YL13-02 (tr1-537) and YL13-03 (trz1-538)] were serially diluted and spotted onto SC-LEU with different sugars added. Plates were incubated at RT (upper), 30°C (middle) and 37°C (lower). |
PMC1156898_F4_2311.jpg | What key item or scene is captured in this photo? | Growth at different temperatures of yeast expressing TRZ1, trz1-537 or trz1-538 alleles on media with various carbon sources added. Yeast bearing TRZ1 alleles integrated at the LEU2 locus [YL13-01 (WT), YL13-02 (tr1-537) and YL13-03 (trz1-538)] were serially diluted and spotted onto SC-LEU with different sugars added. Plates were incubated at RT (upper), 30°C (middle) and 37°C (lower). |
PMC1156918_F1_2313.jpg | What does this image primarily show? | GFP fluorescence. B16 melanoma cells transfected with cystatin C-GFP fusion construct A, B) transiently transfected B16F10C) stably transfected B16F10 cells. |
PMC1156918_F1_2315.jpg | Describe the main subject of this image. | GFP fluorescence. B16 melanoma cells transfected with cystatin C-GFP fusion construct A, B) transiently transfected B16F10C) stably transfected B16F10 cells. |
PMC1156918_F1_2314.jpg | What stands out most in this visual? | GFP fluorescence. B16 melanoma cells transfected with cystatin C-GFP fusion construct A, B) transiently transfected B16F10C) stably transfected B16F10 cells. |
PMC1156920_F1_2319.jpg | What's the most prominent thing you notice in this picture? | Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown. |
PMC1156920_F1_2318.jpg | What does this image primarily show? | Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown. |
PMC1156920_F1_2322.jpg | Describe the main subject of this image. | Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown. |
PMC1156920_F1_2320.jpg | What is the core subject represented in this visual? | Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown. |
PMC1156920_F1_2321.jpg | What can you see in this picture? | Photomicrographs illustrating the pattern of the tumor vascularization. A, The tumor capsule (left) of this PAS stained section reveals blood vessels. The cortex under the capsule reveals no blood vessels and few endothelial pseudopods while the subcortical area to the top right has more pseudopods. B, at higher magnification the subcortical area of the tumor reveals a blood capillary with multiple endothelial pseudopods protruding at right angles into the tumor mass. C, Endothelial pseudopods are seen to branch and occasionally have a vacuole/lumen (arrow). D, The endothelial pseudopods react immunohistochemically positive for the CD-31 specific endothelial cell marker, using the avidin-biotin peroxidase complex method. E, Viable cell area can be seen beneath tumor capsule (left) while necrotic area can be seen to the right. F, Enlarged subcortical area from E. In E, the HIF-α positive area between the viable and the necrotic tissue is stained brown. |
PMC1156922_F1_2316.jpg | What is the core subject represented in this visual? | Cardiac duplex ultrasound image (4-chamber view), obtained 2 days after thrombolysis showing the thrombus attached to left ventricular apex (arrow). |
PMC1156954_F3_2323.jpg | What's the most prominent thing you notice in this picture? | Ultrastructural characteristics of a Coronavirus-Infected cell in BAL fluid from a SARS patient at 60 days, with several intracellular particles. The virions are indicated by the arrowheads in Panel A. Panel B shows the area indicated by the asterisk in Panel A at higher magnification. The bar in Panel A (500 nm) and Panel B (100 nm) is indicated. |
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