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Many of the NGS sequencing protocols rely on the production of a genomic library that contains thousands of fragments of the target nucleic acids that will then be sequenced by proper technologies. According to the sequencing methods to be used, libraries can be created differently (in the case of the Ion Torrent techn...
1
Biochemistry
Kocienski has made contributions to the design and development of new organometallic reagents in synthesis, and the applications of synthetic methods to complex natural products. Early work with Basil Lythgoe on the scope and stereochemistry of the Julia olefination with alpha-metallated sulphone reagents emphasised th...
0
Organic Chemistry
For a pure substance in solution, if the color and path length are fixed and the specific rotation is known, the observed rotation can be used to calculate the concentration. This usage makes a polarimeter a tool of great importance to those trading in or using sugar syrups in bulk.
4
Stereochemistry
Flap endonuclease (FEN) is an endonuclease that catalyzes structure-specific cleavage. This cleavage is highly sensitive to mismatches and can be used to interrogate SNPs with a high degree of specificity In the basic Invader assay, a FEN called cleavase is combined with two specific oligonucleotide probes, that toget...
1
Biochemistry
Cellulose bioplastics are mainly the cellulose esters (including cellulose acetate and nitrocellulose) and their derivatives, including celluloid. Cellulose can become thermoplastic when extensively modified. An example of this is cellulose acetate, which is expensive and therefore rarely used for packaging. However, c...
7
Physical Chemistry
Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules) invented by Miltenyi Biotec. The name MACS is a registered trademark of the company. The method was developed with Miltenyi Biotec's MACS system, which uses superparamagnetic ...
1
Biochemistry
Certain AMGs encode proteins that directly interact with enzymes involved in metabolic reactions. This interaction can either enhance or inhibit enzyme activity, leading to changes in the rate of metabolic flux through specific pathways.
1
Biochemistry
When the required steel had been formed, it was poured into ladles and then transferred into moulds while the lighter slag was left behind. The conversion process, called the "blow", was completed in approximately 20 minutes. During this period, the progress of the oxidation of the impurities was judged by the appearan...
8
Metallurgy
Smoking or vaporizing hash oil is known colloquially as "dabbing", from the English verb to daub (Dutch dabben, French dauber), "to smear with something adhesive". Dabbing devices include special kinds of water pipes ("dab rigs"), vaporizers and vape pens similar in design to electronic cigarettes. Oil rigs include a ...
7
Physical Chemistry
Random amplified polymorphic DNA (RAPD), pronounced "rapid", is a type of polymerase chain reaction (PCR), but the segments of DNA that are amplified are random. The scientist performing RAPD creates several arbitrary, short primers (10–12 nucleotides), then proceeds with the PCR using a large template of genomic DNA,...
1
Biochemistry
Molecular fly-by collisions take little time, something like 10 s. Optical transition of collisional complexes of molecules generate spectral "lines" that are very broad - roughly five orders of magnitude broader than the most familiar "ordinary" spectral lines (Heisenberg's uncertainty relation). The resulting spe...
7
Physical Chemistry
Theoretical quantum-mechanical calculations become rather accurate to describe the energy structure of some simple electronic configurations. The results of theoretical developments were summarized by Condon and Shortley in 1935. Edlén thoroughly analyzed spectra of MIA for many chemical elements and derived regulariti...
7
Physical Chemistry
ANT has long been thought to function as a homodimer, but this concept was challenged by the projection structure of the yeast Aac3p solved by electron crystallography, which showed that the protein was three-fold symmetric and monomeric, with the translocation pathway for the substrate through the centre. The atomic s...
1
Biochemistry
Cupellation is a refining process in metallurgy in which ores or alloyed metals are treated under very high temperatures and subjected to controlled operations to separate noble metals, like gold and silver, from base metals, like lead, copper, zinc, arsenic, antimony, or bismuth, present in the ore. The process is bas...
8
Metallurgy
When a semiconductor comes into contact with a liquid (redox species), to maintain electrostatic equilibrium, there will be a charge transfer between the semiconductor and liquid phase if formal redox potential of redox species lies inside semiconductor band gap. At thermodynamic equilibrium, the Fermi level of semicon...
5
Photochemistry
In order to study trace metal stable isotope biogeochemistry, it is necessary to compare the relative abundances of isotopes of trace metals in a given biological, geological, or chemical pool to a standard (discussed individually for each isotope system below) and monitor how those relative abundances change as a resu...
9
Geochemistry
Copper(I) thiophene-2-carboxylate or CuTC is a coordination complex derived from copper and thiophene-2-carboxylic acid. It is used as a reagent to promote the Ullmann reaction between aryl halides.
0
Organic Chemistry
In organic chemistry, the vanillyl group (also known as vanilloyl) is a functional group. Compounds containing a vanillyl group are called vanilloids, and include vanillin, vanillic acid, capsaicin, vanillylmandelic acid, etc.
0
Organic Chemistry
The introduction of a mechanical bond alters the chemistry of the sub components of rotaxanes and catenanes. Steric hindrance of reactive functionalities is increased and the strength of non-covalent interactions between the components are altered.
6
Supramolecular Chemistry
Supramolecular polymers usually prepared in solution. However anomalous polymeric properties can be expected when these polymers are prepared without a conventional organic or aqueous medium. For instance, liquid crystal media may affect the elementary steps of supramolecular polymerization as demonstrated by Takashi K...
6
Supramolecular Chemistry
The configuration index is a single digit which is defined as the priority number of the ligand on the stem of the "T".
4
Stereochemistry
In 1908, it was reported that exposure of carvone to "Italian sunlight" for one year gives carvone-camphor. Subsequent investigations demonstrated the utility of the photochemical [2+2] cycloaddition of enones to alkenes, requiring only "sunlight in California for 6.5 months".
5
Photochemistry
Primary energy (PE) is the energy found in nature that has not been subjected to any human engineered conversion process. It encompasses energy contained in raw fuels and other forms of energy, including waste, received as input to a system. Primary energy can be non-renewable or renewable. Total primary energy supply ...
7
Physical Chemistry
Compounds or materials that are easily transformed (often by biological activity) are termed labile. For example, labile phosphate is that fraction of soil phosphate that is readily transformed into soluble or plant-available phosphate. Labile organic matter is the soil organic matter that is easily decomposed by micro...
1
Biochemistry
The definitions of VOCs used for control of precursors of photochemical smog used by the U.S. Environmental Protection Agency (EPA) and state agencies in the US with independent outdoor air pollution regulations include exemptions for VOCs that are determined to be non-reactive, or of low-reactivity in the smog formati...
0
Organic Chemistry
Benzeneselenol, also known as selenophenol, is the organoselenium compound with the chemical formula , often abbreviated PhSeH. It is the selenium analog of phenol. This colourless, malodorous compound is a reagent in organic synthesis.
0
Organic Chemistry
Bearing heaters typically range from 1 kVA to 25 kVA and are used to heat parts from , dependent upon the application. The power required is a function of the weight, target temperature and cycle time to aid selection many manufacturers publish graphs and charts.
8
Metallurgy
The mouth is the first part of the upper gastrointestinal tract and is equipped with several structures that begin the first processes of digestion. These include salivary glands, teeth and the tongue. The mouth consists of two regions; the vestibule and the oral cavity proper. The vestibule is the area between the tee...
1
Biochemistry
As typical for the CMGC kinase group, the catalytic site of MAP kinases has a very loose consensus sequence for substrates. Like all their relatives, they only require the target serine / threonine amino acids to be followed by a small amino acid, preferably proline ("proline-directed kinases"). But as SP/TP sites are ...
1
Biochemistry
It is a member of the small pentraxins family (also known as short pentraxins). The polypeptide encoded by this gene has 224 amino acids. The full-length polypeptide is not present in the body in significant quantities due to signal peptide, which is removed by signal peptidase before translation is completed. The comp...
1
Biochemistry
Deacylations "play crucial roles in gene transcription and most likely in all eukaryotic biological processes that involve chromatin". Acetylation is one type of post-translational modification of proteins. The acetylation of the ε-amino group of lysine, which is common, converts a charged side chain to a neutral one....
0
Organic Chemistry
The genes and receptor ligands are as follows: (Hökfelt et al., 2001; Page, 2004; Pennefather et al., 2004; Maggi, 2000)
1
Biochemistry
Staying dry and wearing proper clothing help to prevent hypothermia. Synthetic and wool fabrics are superior to cotton as they provide better insulation when wet and dry. Some synthetic fabrics, such as polypropylene and polyester, are used in clothing designed to wick perspiration away from the body, such as liner soc...
1
Biochemistry
In Complex IV (cytochrome c oxidase; ), sometimes called cytochrome AA3, four electrons are removed from four molecules of cytochrome c and transferred to molecular oxygen (O) and four protons, producing two molecules of water. The complex contains coordinated copper ions and several heme groups. At the same time, eigh...
1
Biochemistry
Knockout mice that completely lack the RIP140 molecule are lean and stay lean, even on a rich diet. Knockout mice (females) are also infertile because they fail to ovulate. Failure of ovulation in these mice is caused by lack of cumulus expansion and altered expression of various genes, including amphiregulin, in ovari...
1
Biochemistry
6-Methoxy-(8-p-toluenesulfonamido)quinoline (TSQ) is one of the most efficient fluorescent stains for zinc(II). It was introduced by Soviet biochemists Toroptsev and Eshchenko in the early 1970s. The popularity of TSQ as physiological stain rose after seminal works by Christopher Frederickson two decades later. TSQ for...
1
Biochemistry
The use of ractopamine in Russia is prohibited. On 6 June 2011, the Russian Ministry of Agriculture notified key meat import/exporters in Russia of a future prohibition of ractopamine in meat imported to Russia. On 7 December 2012, the prohibition went into force, and pork and beef export to Russia required submission ...
4
Stereochemistry
G stimulates the membrane-bound phospholipase C beta, which then cleaves PIP (a minor membrane phosphoinositol) into two second messengers, IP3 and diacylglycerol (DAG). The Inositol Phospholipid Dependent Pathway is used as a signal transduction pathway for many hormones including: * ADH (Vasopressin/AVP) – Induces th...
1
Biochemistry
Surface tension is visible in other common phenomena, especially when surfactants are used to decrease it: * Soap bubbles have very large surface areas with very little mass. Bubbles in pure water are unstable. The addition of surfactants, however, can have a stabilizing effect on the bubbles (see Marangoni effect). Su...
6
Supramolecular Chemistry
Intracranial electrodes consist of conductive electrode arrays implanted on a polymer or silicon, or a wire electrode with an exposed tip and insulation everywhere that stimulation or recording is not desired. Biocompatibility is essential for the entire implant, but special attention is paid to the actual electrodes s...
7
Physical Chemistry
Whether or not we say a transition between a state and a state is allowed often depends on how detailed the model is that we use for the atom-light interaction. From a particular model follow a set of selection rules that determine which transitions are allowed and which are not. Often these selection rules can be bo...
7
Physical Chemistry
Outdoor air may contain low levels of benzene from automobile service stations, wood smoke, tobacco smoke, the transfer of gasoline, exhaust from motor vehicles, and industrial emissions. About 50% of the entire nationwide (United States) exposure to benzene results from smoking tobacco or from exposure to tobacco smok...
2
Environmental Chemistry
The role of vitamin A in the visual cycle is specifically related to the retinal compound. Retinol is converted by the enzyme RPE65 within the retinal pigment epithelium into 11-cis-retinal. Within the eye, 11-cis-retinal is bound to the protein opsin to form rhodopsin in rod cells and iodopsin in cone cells. As light ...
1
Biochemistry
Many alcohols are produced by hydroxylation, i.e., the installation of a hydroxy group using oxygen or a related oxidant. Hydroxylation is the means by which the body processes many poisons, converting lipophilic compounds into hydrophilic derivatives that are more readily excreted. Enzymes called hydroxylases and ox...
0
Organic Chemistry
A plasmonic antenna-reactor photocatalyst is a photocatalyst that combines a catalyst with attached antenna that increases the catalyst's ability to absorb light, thereby increasing its efficiency. A Silicon dioxide| catalyst combined with an Au light absorber accelerated hydrogen sulfide-to-hydrogen reactions. The pro...
5
Photochemistry
In combustion, Zeldovich–Liñán model is a two-step reaction model for the combustion processes, named after Yakov Borisovich Zeldovich and Amable Liñán. The model includes a chain-branching and a chain-breaking (or radical recombination) reaction. The model was first introduced by Zel'dovich in 1948 and later analysed ...
7
Physical Chemistry
The copper coulometer is a one application for the copper-copper(II) sulfate electrode. Such a coulometer consists of two identical copper electrodes immersed in slightly acidic pH-buffered solution of copper(II) sulfate. Passing of current through the element leads to the anodic dissolution of the metal on anode and s...
7
Physical Chemistry
The petite is characterized by a deficiency in cytochromes (a, a3 + b) and a lack of respiratory enzymes which engage in respiration in mitochondria. Due to the error in the respiratory chain pathway, petite yeast is incapable of growing on media containing only non-fermentable carbon sources (such as glycerol or ethan...
1
Biochemistry
*X for any (pseudo)halogen atom (in the related MLXZ notation, X represents a one-electron donor ligand) *L or L for a ligand or ligands (in the related MLXZ notation, L represents a two-electron donor ligand) *M or Met for any metal atom ([M] is used to indicate a ligated metal, ML, when the identities of the ligands ...
0
Organic Chemistry
The adverse effects of lithium include: ;Very Common (> 10% incidence) adverse effects * Confusion * Constipation (usually transient, but can persist in some) * Decreased memory * Diarrhea (usually transient, but can persist in some) * Dry mouth * EKG changes — usually benign changes in T waves * Hand tremor (usually t...
1
Biochemistry
This trend is also generally true of pentacoordinated main-group elements with one or more lone-pair-containing ligand, including the oxygen-pentacoordinated silicon examples shown below. The Si-halogen bonds range from close to the expected van der Waals value in A (a weak bond) almost to the expected covalent single ...
4
Stereochemistry
Medical organizations recommend that patients protect themselves from UV radiation by using sunscreen. Five sunscreen ingredients have been shown to protect mice against skin tumors. However, some sunscreen chemicals produce potentially harmful substances if they are illuminated while in contact with living cells. The ...
5
Photochemistry
In immunology, an antigen (Ag) is a molecule, moiety, foreign particulate matter, or an allergen, such as pollen, that can bind to a specific antibody or T-cell receptor. The presence of antigens in the body may trigger an immune response. Antigens can be proteins, peptides (amino acid chains), polysaccharides (chains...
1
Biochemistry
SBO is currently made up of seven different vocabularies: * systems description parameter (catalytic constant, thermodynamic temperature...) * participant role (substrate, product, catalyst...) * modelling framework (discrete, continuous...) * mathematical expression (mass-action rate law, Hill-type rate law...) * occu...
1
Biochemistry
Methyl violet is a family of organic compounds that are mainly used as dyes. Depending on the number of attached methyl groups, the color of the dye can be altered. Its main use is as a purple dye for textiles and to give deep violet colors in paint and ink. It is also used as a hydration indicator for silica gel. Meth...
3
Analytical Chemistry
With surface chemistries that are weakly ionic, the choice of pH can affect the ionic nature of the column chemistry. Properly adjusted, the pH can be set to reduce the selectivity toward functional groups with the same charge as the column, or enhance it for oppositely charged functional groups. Similarly, the choice ...
1
Biochemistry
Exemestane has been used in doping to raise luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels, which in turn increases the ratio of male over female sexual hormones and so improves performance. The drug also counteracts gynecomastia as well as fat and water retention following excessive aromatase p...
4
Stereochemistry
General purpose diagrams include: *PV diagram *T–s diagram *h–s (Mollier) diagram *Psychrometric chart *Cooling curve *Indicator diagram *Saturation vapor curve *Thermodynamic surface Specific to weather services, there are mainly three different types of thermodynamic diagrams used: * Skew-T log-P diagram * Tephigram ...
7
Physical Chemistry
Because one of the main purposes for biomedical engineering is to mimic body parts to sustain normal body functions, due to their biocompatible properties, biopolymers are used vastly for tissue engineering, medical devices and the pharmaceutical industry. Many biopolymers can be used for regenerative medicine, tissue ...
1
Biochemistry
Although m6A sites could be profiled at high resolution using UV-based methods, the stoichiometry of m6A sites - the methylation status or the ratio m6A+ to m6A- for each individual site within a type of RNA - is still unknown. SCARLET (2013) and m6A-LAIC-seq (2016) allows for the quantitation of stoichiometry at a spe...
1
Biochemistry
Sulfuric acid is capable of causing very severe burns, especially when it is at high concentrations. In common with other corrosive acids and alkali, it readily decomposes proteins and lipids through amide and ester hydrolysis upon contact with living tissues, such as skin and flesh. In addition, it exhibits a strong d...
7
Physical Chemistry
* Culliford, Bryan J., The Examination and Typing of Bloodstains in the Crime Laboratory, Washington, D.C.: U.S. Government Printing Office, 1971. * Gaensslen, Robert E., Sourcebook in Forensic Serology, Immunology, and Biochemistry, Washington, D.C.: U.S. Government Printing Office, 1983. * Kirk, Paul L., Crime Invest...
3
Analytical Chemistry
Biohydrometallurgy is a technique in the world of metallurgy that utilizes biological agents (bacteria) to recover and treat metals such as copper. Modern biohydrometallurgy advances started with the bioleaching of copper more efficiently in the 1950's
8
Metallurgy
CKLF like MARVEL transmembrane domain-containing 8 (i.e. CMTM8), previously termed chemokine-like factor superfamily 8 (i.e. CKLFSF8) has at least two isoforms, the CMTM8 and CMTM8-v2 proteins. Protein isoforms are variant products that are made by the alternative splicing of a single gene. The gene for these isoforms,...
1
Biochemistry
It has been proposed that mutations in gatekeeper genes could, to an extent, offer a sort of selective advantage to the individual in which the change occurs. This is because cells with these mutations are able to replicate at a faster rate than nearby cells. This is known as "increased somatic fitness". Caretaker g...
1
Biochemistry
In the US, where the Midrex process was first developed, direct reduction was seen in the 1960s as a way of breathing new life into electric steelmaking. The techno-economic model of the mini-mill, based on flexibility and reduced plant size, was threatened by a shortage of scrap metal, and a consequent rise in its pri...
8
Metallurgy
The first discussion of obtaining an image of a surface using atoms was by King and Bigas, who showed that an image of a surface can be obtained by heating a sample and monitoring the atoms that evaporate from the surface. King and Bigas suggest that it could be possible to form an image by scattering atoms from the su...
7
Physical Chemistry
In early 1900, Max Trautz and William Lewis studied the rate of the reaction using collision theory, based on the kinetic theory of gases. Collision theory treats reacting molecules as hard spheres colliding with one another; this theory neglects entropy changes, since it assumes that the collision between molecules ar...
7
Physical Chemistry
Immunofluorescence (IF) on ethanol-fixed neutrophils is used to detect ANCA, although formalin-fixed neutrophils may be used to help differentiate ANCA patterns. ANCA can be divided into four patterns when visualised by IF; cytoplasmic ANCA (c-ANCA), C-ANCA (atypical), perinuclear ANCA (p-ANCA) and atypical ANCA (a-ANC...
1
Biochemistry
The hydrolysis reaction for a hydrated metal ion in aqueous solution can be written as: :p M + q HO ⇌ M(OH) + q H and the corresponding formation constant as: and associated equilibria can be written as: :MO(OH)(s) + z H ⇌ M + (z–x) HO :MO(OH)(s) + x HO ⇌ M + z OH :p MO(OH)(s) + (pz–q) H ⇌ M(OH) + (pz–px–q) HO
7
Physical Chemistry
MFGM may be capable of modulating immune function in the gut through distinct but potentially complementary mechanisms. Glycosylated proteins (MUC-1, MUC-15, butyrophilin, and lactadherin) and glycosylated sphingolipids from MFGM may promote the development of healthy gut microbiota by favoring beneficial Bifidobacteri...
1
Biochemistry
no large clinical trials of CoQ in cancer treatment had been conducted. The US's National Cancer Institute identified issues with the few, small studies that had been carried out, stating, "the way the studies were done and the amount of information reported made it unclear if benefits were caused by the CoQ or by some...
1
Biochemistry
Gel electrophoresis is a method for separation and analysis of biomacromolecules (DNA, RNA, proteins, etc.) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology t...
1
Biochemistry
eIF2B is a protein complex found in eukaryotes. It is the guanine nucleotide exchange factor for the eukaryotic initiation factor 2 and therefore converts the inactive eIF2-GDP to the active eIF2-GTP. This activation is hindered by phosphorylation of the alpha subunit of eIF2, which leads to a stable eIF2α-P-GDP-eIF2B ...
1
Biochemistry
Global sensitivity measures such as the Hill coefficient do not characterise the local behaviours of the s-shaped curves. Instead, these features are well captured by the response coefficient measure defined as: In systems biology, such responses are referred to as elasticities.
1
Biochemistry
If water is left to stand in a tube for an extended period, the chemical characteristics of the water change as the mixed scale and corrosion products are deposited. In addition any loose scale that is not well adhered to the wall will not be flushed away and air dissolved in the water will form bubbles, producing air ...
8
Metallurgy
In X-ray crystallography, crystallographic disorder describes the cocrystallization of more than one rotamer, conformer, or isomer where the center of mass of each form is identical or unresolvable. As a consequence of disorder, the crystallographic solution is the sum of the various forms. In many cases, the compone...
3
Analytical Chemistry
This section contains a discussion of the three most important properties of time reversal in quantum mechanics; chiefly, # that it must be represented as an anti-unitary operator, # that it protects non-degenerate quantum states from having an electric dipole moment, # that it has two-dimensional representations with ...
7
Physical Chemistry
Nitrene reactions include: * Nitrene C–H insertion. A nitrene can easily insert into a carbon to hydrogen covalent bond yielding an amine or amide. A singlet nitrene reacts with retention of configuration. In one study a nitrene, formed by oxidation of a carbamate with potassium persulfate, gives an insertion reaction...
0
Organic Chemistry
In analytical chemistry, sub-sampling is a procedure by which a small, representative sample is taken from a larger sample. Good sub-sampling technique becomes important when the large sample is not homogeneous.
3
Analytical Chemistry
Electrical properties include both electrical resistivity and conductivity, dielectric strength, electromagnetic permeability, and permittivity. Electrical conductors such as metals and alloys are contrasted with electrical insulators such as glasses and ceramics. Semiconductors behave somewhere in between. Whereas con...
7
Physical Chemistry
The N-phenylimino derivative of CDI can be formed in a Wittig-like reaction with triphenylphosphine phenylimide. :OCIm + PhP=NPh → PhN=CIm + PhPO CDI can act as a carbonyl equivalent in the formation of tetronic acids or pulvinones from hydroxyketones and diketones in basic conditions. An alcohol treated with at...
0
Organic Chemistry
Sodium dithiophosphate is the salt with the formula NaPSO. It is usually supplied as the hydrated solid or as an aqueous solution together with other thiophosphates such as sodium monothiophosphate and sodium trithiophosphate. It is a colorless compound, but commercial samples can appear dark owing to the presence of...
8
Metallurgy
In an ideal conductor, where atoms are arranged in a perfect lattice structure, the electrons moving through it would experience no collisions and electromigration would not occur. In real conductors, defects in the lattice structure and the random thermal vibration of the atoms about their positions causes electrons ...
7
Physical Chemistry
Glycogenin-1 is an enzyme that is involved in the biosynthesis of glycogen. It is capable of self-glucosylation, forming an oligosaccharide primer that serves as a substrate for glycogen synthase. This is done through an inter-subunit mechanism. It also plays a role in glycogen metabolism regulation. Recombinant human ...
1
Biochemistry
Embryo transfer can be performed after various durations of embryo culture, conferring different stages in embryogenesis. The main stages at which embryo transfer is performed are cleavage stage (day 2 to 4 after co-incubation) or the blastocyst stage (day 5 or 6 after co-incubation). Because in vivo, a cleavage stage ...
1
Biochemistry
For each uniform structure, there also exists a related but different structure, called a line-slip arrangement. The differences between uniform and line-slip structures are marginal and difficult to spot from images of the sphere packings. However, by comparing their rolled-out contact networks, one can spot that cert...
3
Analytical Chemistry
Alaska had fewer than 4,000 gallons of dispersants available at the time of the Exxon Valdez oil spill, and no aircraft with which to dispense them. The dispersants introduced were relatively ineffective due to insufficient wave action to mix the oil and water, and their use was shortly abandoned. A report by David Kir...
2
Environmental Chemistry
The CBP of this type, also known as parG is composed of: * N-terminal NTPase binding domain * Ribon-Helix-Helix (RHH) domain For this type, the parS site is called parC.
1
Biochemistry
Siefert writes: : proved a remarkable relation which allows to express the free energy difference between two equilibrium systems by a nonlinear average over the work required to drive the system in a non-equilibrium process from one state to the other. By comparing probability distributions for the work spent in the o...
7
Physical Chemistry
The energetic requirements of a body are composed of the basal metabolic rate (BMR) and the physical activity level (ERAT, exercise-related activity thermogenesis). This caloric requirement can be met with protein, fat, carbohydrates, or a mixture of those. Glucose is the general metabolic fuel, and can be metabolized...
1
Biochemistry
Atmospheric nitrogen is inaccessible to most organisms, because its triple covalent bond is very strong. Most take up fixed nitrogen from various sources. For every 100 atoms of carbon, roughly 2 to 20 atoms of nitrogen are assimilated. The atomic ratio of carbon (C) : nitrogen (N) : phosphorus (P) observed on average ...
1
Biochemistry
Amyloid-beta precursor protein (APP) is an integral membrane protein expressed in many tissues and concentrated in the synapses of neurons. It functions as a cell surface receptor and has been implicated as a regulator of synapse formation, neural plasticity, antimicrobial activity, and iron export. It is coded for by ...
1
Biochemistry
Putrefaction is the fifth stage of death, following pallor mortis, livor mortis, algor mortis, and rigor mortis. This process references the breaking down of a body of an animal post-mortem. In broad terms, it can be viewed as the decomposition of proteins, and the eventual breakdown of the cohesiveness between tissues...
1
Biochemistry
Levofenfluramine (INN), or (−)-3-trifluoromethyl-N-ethylamphetamine, also known as (−)-fenfluramine or (R)-fenfluramine, is a drug of the amphetamine family that, itself (i.e., in enantiopure form), was never marketed. It is the levorotatory enantiomer of fenfluramine, the racemic form of the compound, whereas the dex...
4
Stereochemistry
The first potentiometric titration was carried out in 1893 by Robert Behrend at Ostwald's Institute in Leipzig. He titrated mercurous solution with potassium chloride, potassium bromide, and potassium iodide. He used a mercury electrode along with a mercury/mercurous nitrate reference electrode. He found that in a cell...
3
Analytical Chemistry
Progesterone binds extensively to plasma proteins, including albumin (50–54%) and transcortin (43–48%). It has similar affinity for albumin relative to the PR.
0
Organic Chemistry
For ice, recrystallization refers to the growth of larger crystals at the expense of smaller ones. Some biological antifreeze proteins have been shown to inhibit this process, and the effect may be relevant in freezing-tolerant organisms.
3
Analytical Chemistry
Leaves which have a petiole (leaf stalk) are said to be petiolate. Sessile (epetiolate) leaves have no petiole and the blade attaches directly to the stem. Subpetiolate leaves are nearly petiolate or have an extremely short petiole and may appear to be sessile. In clasping or decurrent leaves, the blade partially surro...
5
Photochemistry
Theodor W. Hänsch and John L. Hall shared half of the 2005 Nobel Prize in Physics for contributions to the development of laser-based precision spectroscopy, including the optical frequency-comb technique. The other half of the prize was awarded to Roy Glauber. Also in 2005, the femtosecond comb technique was extended ...
7
Physical Chemistry