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PMID:20227 | Production of calcitonin, adrenocorticotropic hormone, and beta-melanocyte-stimulating hormone in tumors derived from amine precursor uptake and decarboxylation cells. | The tumor production of human calcitonin (CT) was examined by radioimmunoassay, and it was found that 50 of 85 (59%) tumor tissues collected at random contained immunoreactive CT. These tumors were grouped as to whether they were derived from the amine precursor uptake and decarboxylation (APUD) series. The group that ... | Production of calcitonin, adrenocorticotropic hormone, and beta-melanocyte-stimulating hormone in tumors derived from amine precursor uptake and decarboxylation cells. The tumor production of human calcitonin (CT) was examined by radioimmunoassay, and it was found that 50 of 85 (59%) tumor tissues collected at random c... | [
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PMID:20228 | The use of titanium (IV) oxide for the immobilisation of carbohydrate-directed enzymes. | The use of particles of porous titanium (IV) oxide as a suitable matrix for enzyme immobilisation has been investigated with dextranase. Treatment of the particles with enzyme in the presence and absence of ammonium ions showed that the presence of ammonia induced a greater coupling of protein, whereas a greater retent... | The use of titanium (IV) oxide for the immobilisation of carbohydrate-directed enzymes. The use of particles of porous titanium (IV) oxide as a suitable matrix for enzyme immobilisation has been investigated with dextranase. Treatment of the particles with enzyme in the presence and absence of ammonium ions showed that... | [
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PMID:20239 | [Regulation and electric excitation of enzyme membranes in vitro]. | Enzyme membranes can be activated or inhibited by applying continuous or alternating electrical fields. The field can modify the transport or reaction term of the transport-reaction by action on the displacement of charged species including those giving pH effects or inducing volume flows. A first experimental example ... | [Regulation and electric excitation of enzyme membranes in vitro]. Enzyme membranes can be activated or inhibited by applying continuous or alternating electrical fields. The field can modify the transport or reaction term of the transport-reaction by action on the displacement of charged species including those giving... | [
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PMID:20246 | A kinetic spectrophotometric assay for rapid determination of acetoacetate in blood. | We describe an automated kinetic assay for acetoacetate in blood. Acetoacetate is enzymatically reduced to D-beta-hydroxybutyrate and the reaction is monitored for 60 s with a reaction-rate analyzer. This technique allows low concentrations of acetoacetate to be measured with good precision and overcomes many of the pr... | A kinetic spectrophotometric assay for rapid determination of acetoacetate in blood. We describe an automated kinetic assay for acetoacetate in blood. Acetoacetate is enzymatically reduced to D-beta-hydroxybutyrate and the reaction is monitored for 60 s with a reaction-rate analyzer. This technique allows low concentra... | [
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PMID:20247 | Automated kinetic determination of lactate dehydrogenase isoenzymes in serum. | A steady-state kinetic method has been revised for measuring lactate dehydrogenase isoenzyme activities, which relates the inhibition of heart-type isoenzyme activity to the overall isoenzyme composition of the enzyme subunits. The method depends on the pH-dependent formation of an inhibitory ternary complex by the hea... | Automated kinetic determination of lactate dehydrogenase isoenzymes in serum. A steady-state kinetic method has been revised for measuring lactate dehydrogenase isoenzyme activities, which relates the inhibition of heart-type isoenzyme activity to the overall isoenzyme composition of the enzyme subunits. The method dep... | [
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PMID:20250 | Determination of free and esterified cholesterol by a kinetic method. I. The introduction of the enzymatic method with 2,2'-azino-di-3[ethyl-benzthiazolin sulfonic acid (6)] (ABTS). | A new, simple kinetic method is described for the determination of serum cholesterol based on the oxidation of 2,2'-azino-di-3[ethyl-benzthiazolin sulfonic acid (6)] (ABTS) by use of a single aqueous reagent. This assay procedure is rapid, specific, reproducible and applicable to the measurement of free and esterified ... | Determination of free and esterified cholesterol by a kinetic method. I. The introduction of the enzymatic method with 2,2'-azino-di-3[ethyl-benzthiazolin sulfonic acid (6)] (ABTS). A new, simple kinetic method is described for the determination of serum cholesterol based on the oxidation of 2,2'-azino-di-3[ethyl-benzt... | [
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PMID:20251 | [Concanavalin A- Sepharose affinity chromatography for routine microanalysis of gamma-glutamyltransferase (author's transl)]. | The separation of two molecular forms of liver gamma-glutamyltransferase is achieved by Con A-Sepharose chromatography, an adult type with high affinity to Con A and a fetal type without binding capacity to this lectin. Now we present a new method using micro-columns for affinity chromatography (gel volume 2 ml). By th... | [Concanavalin A- Sepharose affinity chromatography for routine microanalysis of gamma-glutamyltransferase (author's transl)]. The separation of two molecular forms of liver gamma-glutamyltransferase is achieved by Con A-Sepharose chromatography, an adult type with high affinity to Con A and a fetal type without binding... | [
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PMID:20252 | Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. | We describe a new assay that is useful for identifying individuals who may be affected with Gaucher's disease. The assay involves the determination of serum acid phosphatase activity using the fluorogenic substrate 4-methylumbelliferyl phosphate. The assay measures acid phosphatase activity at pH 6.0 in the presence of... | Determination of serum acid phosphatase in Gaucher's disease using 4-methylumbelliferyl phosphate. We describe a new assay that is useful for identifying individuals who may be affected with Gaucher's disease. The assay involves the determination of serum acid phosphatase activity using the fluorogenic substrate 4-meth... | [
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PMID:20253 | New enzymatic assay of cholinesterase activity. | A new enzymatic method for the determination of serum pseudo-cholinesterase activity is described. Choline, which is liberated from benzoylcholine as substrate by cholinesterase, is oxidized by choline oxidase to betaine with the simultaneous production of hydrogen peroxide, which oxidatively couples with 4-aminoantipy... | New enzymatic assay of cholinesterase activity. A new enzymatic method for the determination of serum pseudo-cholinesterase activity is described. Choline, which is liberated from benzoylcholine as substrate by cholinesterase, is oxidized by choline oxidase to betaine with the simultaneous production of hydrogen peroxi... | [
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PMID:20255 | Quantitative elution studies in experimental immune complex and nephrotoxic nephritis. | Optimal conditions have been determined for elution of antiglomerular basement-membrane antibodies and antigen-antibody complexes from nephritic kidneys, obtained from rabbits with nephrotoxic nephritis and various forms of BSA-induced immune complex disease. Elution from kidney homogenates was performed with acid and ... | Quantitative elution studies in experimental immune complex and nephrotoxic nephritis. Optimal conditions have been determined for elution of antiglomerular basement-membrane antibodies and antigen-antibody complexes from nephritic kidneys, obtained from rabbits with nephrotoxic nephritis and various forms of BSA-induc... | [
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PMID:20256 | Cyclic 3'5'-adenosine monophosphate and central circulatory control in cats and dogs. | 1. Dibutyryl cyclic AMP (Db cAMP, 75-500 microgram/kg), injected into the lateral ventricle of the brain of the cat increased blood pressure, heart rate and splanchnic discharge rate. 2. ATP, but not AMP, induced similar changes; GMP in small doses increased blood pressure. 3. A number of drugs are known to activate ad... | Cyclic 3'5'-adenosine monophosphate and central circulatory control in cats and dogs. 1. Dibutyryl cyclic AMP (Db cAMP, 75-500 microgram/kg), injected into the lateral ventricle of the brain of the cat increased blood pressure, heart rate and splanchnic discharge rate. 2. ATP, but not AMP, induced similar changes; GMP ... | [
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PMID:20261 | Effects of urine acidification on plasma and urine phencyclidine levels in overdosage. | A gas chromatography--mass fragmentography--electron impact (GC-MS-EI) assay of phencyclidine (PCP) was adapted for human plasma and urine. This assay is specific for PCP and very sensitive (approximately 1 ng/ml). Patients with the putative diagnosis of PCP overdosage were studied to correlate plasma and urinary level... | Effects of urine acidification on plasma and urine phencyclidine levels in overdosage. A gas chromatography--mass fragmentography--electron impact (GC-MS-EI) assay of phencyclidine (PCP) was adapted for human plasma and urine. This assay is specific for PCP and very sensitive (approximately 1 ng/ml). Patients with the ... | [
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PMID:20283 | [Modified agar diffusion test for direct testing of the effectiveness of released antibiotics from bone cements]. | A modified agar-diffusion method is used to test the bactericidal efficacy of antibacterial substances (model substance: Gentamicin) that have been mixed with Palacos osseous cement. Holes of a diameter slightly larger than the diameter of the cylindric Palacos test block are cut from the agar plate. The remaining spac... | [Modified agar diffusion test for direct testing of the effectiveness of released antibiotics from bone cements]. A modified agar-diffusion method is used to test the bactericidal efficacy of antibacterial substances (model substance: Gentamicin) that have been mixed with Palacos osseous cement. Holes of a diameter sli... | [
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PMID:20284 | Clorazepate: double blind crossover comparison of a single nightly dose with diazepam thrice daily in anxiety. | In a double blind cross-over study in 40 patients with mild to moderate anxiety, clorazepate 15 mg at bedtime was as effective as diazepam on global rating and slightly superior on target symptom assessment. There was a significantly higher incidence and frequency of side effects during diazepam treatment. Occurrence o... | Clorazepate: double blind crossover comparison of a single nightly dose with diazepam thrice daily in anxiety. In a double blind cross-over study in 40 patients with mild to moderate anxiety, clorazepate 15 mg at bedtime was as effective as diazepam on global rating and slightly superior on target symptom assessment. T... | [
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PMID:20291 | Effect of dose on the pharmacokinetics of intravenous nicotine in the rat. | The pharmacokinetics of [methyl-14C]nicotine were evaluated in male Fischer-344 rats following the iv injection of 0.08-, 0.4-, or 0.8-mg/kg doses. Independent of dose, the concentration of nicotine declined biexponentially with a mean half-life ranging from 0.92 to 1.10 hr. However, the total plasma clearance of nicot... | Effect of dose on the pharmacokinetics of intravenous nicotine in the rat. The pharmacokinetics of [methyl-14C]nicotine were evaluated in male Fischer-344 rats following the iv injection of 0.08-, 0.4-, or 0.8-mg/kg doses. Independent of dose, the concentration of nicotine declined biexponentially with a mean half-life... | [
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PMID:20292 | Pharmacokinetics of 3,3',5,5'-tetrachlorobiphenyl in the male rat. | The pharmacokinetics of 3,3',5,5'-tetrachlorobiphenyl (4-CB) was studied to determine the importance of chlorine position to the kinetics of polychlorinated biphenyl (PCB) distribution, metabolism, and excretion by the rat. Tissue samples and excreta were collected at times ranging from 2 hr to 42 days after intravenou... | Pharmacokinetics of 3,3',5,5'-tetrachlorobiphenyl in the male rat. The pharmacokinetics of 3,3',5,5'-tetrachlorobiphenyl (4-CB) was studied to determine the importance of chlorine position to the kinetics of polychlorinated biphenyl (PCB) distribution, metabolism, and excretion by the rat. Tissue samples and excreta we... | [
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PMID:20293 | Alteration of in vivo zoxazolamine metabolism by carbon monoxide in normal and polycyclic hydrocarbon-treated immature male rats. | The effect of carbon monoxide exposure on the in vivo metabolism of zoxazolamine in normal (corn oil-treated) and 3,4-benzpyrene- and 3-methylcholanthrene-treated, immature, male rats was examined. Pre-exposure of the animals for 90 min followed by determination of the duration of drug action while the animals were ma... | Alteration of in vivo zoxazolamine metabolism by carbon monoxide in normal and polycyclic hydrocarbon-treated immature male rats. The effect of carbon monoxide exposure on the in vivo metabolism of zoxazolamine in normal (corn oil-treated) and 3,4-benzpyrene- and 3-methylcholanthrene-treated, immature, male rats was e... | [
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PMID:20303 | Developmental aspects of xenobiotic transformation. | In most laboratory animals monooxygenases are apparently absent or barely detectable in fetal organs until just before birth. In this contribution hepatic cytochrome P-450-dependent reactions in the rat are considered only. The results are interpreted on basis of the reaction scheme of Estabrook. To avoid methodologica... | Developmental aspects of xenobiotic transformation. In most laboratory animals monooxygenases are apparently absent or barely detectable in fetal organs until just before birth. In this contribution hepatic cytochrome P-450-dependent reactions in the rat are considered only. The results are interpreted on basis of the ... | [
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PMID:20304 | A kinetic study of the pH-dependent properties of the ferric undecapeptide of cytochrome c (microperoxidase). | The ferric form of the haem undecapeptide, derived from horse cytochrome c by peptic digestion, undergoes at least three pH-induced transitions with pK values of 3.4, 5.8 and 7.6. Temperature-jump experiments suggest that the first of these is due to the binding of a deprotonated imidazole group to the feric iron while... | A kinetic study of the pH-dependent properties of the ferric undecapeptide of cytochrome c (microperoxidase). The ferric form of the haem undecapeptide, derived from horse cytochrome c by peptic digestion, undergoes at least three pH-induced transitions with pK values of 3.4, 5.8 and 7.6. Temperature-jump experiments s... | [
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PMID:20305 | The role of cytochrome P-450 in the hydroperoxide-catalyzed oxidation of alcohols by rat-liver microsomes. | The organic hydroperoxide cumene hydroperoxide is capable of oxidizing ethanol to acetaldehyde in the presence of either catalase, purified cytochrome P-450 or rat liver microsomes. Other hemoproteins like horseradish peroxidase, cytochrome c or hemoglobin were ineffective. In addition to ethanol, higher alcohols like ... | The role of cytochrome P-450 in the hydroperoxide-catalyzed oxidation of alcohols by rat-liver microsomes. The organic hydroperoxide cumene hydroperoxide is capable of oxidizing ethanol to acetaldehyde in the presence of either catalase, purified cytochrome P-450 or rat liver microsomes. Other hemoproteins like horsera... | [
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PMID:20307 | Kinetics of sheep-liver cytoplasmic aldehyde dehydrogenase. | 1. Sheep liver cytoplasmic aldehyde dehydrogenase showed little pH dependence of V or kcat. Some buffer anion effects were noted. 2. The oxidation of aldehydes at pH 7.6 was quantitative but irreversible. The initial velocity data indicated a sequential mechanism for the addition of substrates. Inhibition by NADH and t... | Kinetics of sheep-liver cytoplasmic aldehyde dehydrogenase. 1. Sheep liver cytoplasmic aldehyde dehydrogenase showed little pH dependence of V or kcat. Some buffer anion effects were noted. 2. The oxidation of aldehydes at pH 7.6 was quantitative but irreversible. The initial velocity data indicated a sequential mechan... | [
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PMID:20308 | Maldescent of the testes--an epidemiological study. | The case histories of 2,362 newborn boys were evaluated for congenital testicular maldescent. These data, obtained from the prospective follow-up study "Pregnancy and Child Development" sponsored by the Deutsche Forschungsgemeinschaft, are discussed in the light of new findings on morphological changes in this disorder... | Maldescent of the testes--an epidemiological study. The case histories of 2,362 newborn boys were evaluated for congenital testicular maldescent. These data, obtained from the prospective follow-up study "Pregnancy and Child Development" sponsored by the Deutsche Forschungsgemeinschaft, are discussed in the light of ne... | [
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PMID:20309 | Adult height of patients treated in childhood for undescended testes. | 101 patients successfully treated for undescended testes with human chorionic gonadotrophin (HCG) or with HCG followed by surgery reached normal adult heights. Deviations from normal growth in individual patients did not appear to be related either to their earlier undescended testes or to the successful therapy, but r... | Adult height of patients treated in childhood for undescended testes. 101 patients successfully treated for undescended testes with human chorionic gonadotrophin (HCG) or with HCG followed by surgery reached normal adult heights. Deviations from normal growth in individual patients did not appear to be related either t... | [
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PMID:20310 | Adult metachromatic leukodystrophy. I. Clinical manifestation in a female aged 44 years, previously diagnosed in the preclinical state. | In a 5-year follow-up of a case of adult metachromatic leukodystrophy, already diagnosed in the preclinical stage, the development of the symptoms of this disease could be studied in detail: initially, lack of drive, emotional lability and depressive mood. At the same time, pain in the arms and beginning gait disturban... | Adult metachromatic leukodystrophy. I. Clinical manifestation in a female aged 44 years, previously diagnosed in the preclinical state. In a 5-year follow-up of a case of adult metachromatic leukodystrophy, already diagnosed in the preclinical stage, the development of the symptoms of this disease could be studied in d... | [
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PMID:20311 | Adult metachromatic leukodystrophy. II. Ultrastructural findings in peripheral nerve and skeletal muscle. | Sural nerve biopsy in a 44-year-old woman with adult metachromatic leukodystrophy (MLD) confirmed by deficient arylsulfatase-A activity, showed a reduction in the number of large and small myelinated axons, and sparse metachromatic material. Ultrastructurally, the latter consisted of various types of residual bodies in... | Adult metachromatic leukodystrophy. II. Ultrastructural findings in peripheral nerve and skeletal muscle. Sural nerve biopsy in a 44-year-old woman with adult metachromatic leukodystrophy (MLD) confirmed by deficient arylsulfatase-A activity, showed a reduction in the number of large and small myelinated axons, and spa... | [
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PMID:20312 | Carbohydrase activity in the digestive system of some teleost fishes. | The activity of carbohydrases in Puntius sophore (Ham.), Channa gachua (Ham.) and Cirrhinus mrigala (Ham.) has been studied. The carbohydrases have been found in the stomach, intestinal bulb, intestine, pyloric caeca and the hepato-pancreas. The hepatopancreas is the main site of production of these enzymes and it is i... | Carbohydrase activity in the digestive system of some teleost fishes. The activity of carbohydrases in Puntius sophore (Ham.), Channa gachua (Ham.) and Cirrhinus mrigala (Ham.) has been studied. The carbohydrases have been found in the stomach, intestinal bulb, intestine, pyloric caeca and the hepato-pancreas. The hepa... | [
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PMID:20313 | Aminotripeptidase (EC E.4.1.3) activity in the digestive system of some teleost fishes. | Aminotripeptidase (EC 3.4.1.3) has been found to be present in the intestinal mucosa, hepatopancreas and the pyloric caeca of the three fished examined. The pH optimum of the enzyme ranges from 6.6 to 7.6. The pH optimum varies from species to species and also from section to section of the gut in the same species. The... | Aminotripeptidase (EC E.4.1.3) activity in the digestive system of some teleost fishes. Aminotripeptidase (EC 3.4.1.3) has been found to be present in the intestinal mucosa, hepatopancreas and the pyloric caeca of the three fished examined. The pH optimum of the enzyme ranges from 6.6 to 7.6. The pH optimum varies from... | [
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PMID:20314 | The interaction of 1-(2(diarylmethoxy)ethyl)aziridines with histamine receptors in the longitudinal muscle strip of the guinea pig ileum. | The time course of the onset and decline of histamine antagonism by 1-(2-(diarylmethoxy)ethyl)aziridines, compounds which could be expected to have H1-receptor alkylating properties, was investigated on the longitudinal muscle strip of the guinea pig ileum. Experiments were performed with normal preparations and with m... | The interaction of 1-(2(diarylmethoxy)ethyl)aziridines with histamine receptors in the longitudinal muscle strip of the guinea pig ileum. The time course of the onset and decline of histamine antagonism by 1-(2-(diarylmethoxy)ethyl)aziridines, compounds which could be expected to have H1-receptor alkylating properties,... | [
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PMID:20315 | [Comparative biochemical studies of placental alkaline phosphatase in animals (author's transl)]. | Biochemical properties of alkaline phosphatase (ALP) from placenta were compared between man, dog, cat, rabbit and cattle. 1) Optimum pH of the enzyme was essentially identical through the species of the animals but the inhibition of L-phenylalanine was clearly demonstrable with human ALP but little with that of other ... | [Comparative biochemical studies of placental alkaline phosphatase in animals (author's transl)]. Biochemical properties of alkaline phosphatase (ALP) from placenta were compared between man, dog, cat, rabbit and cattle. 1) Optimum pH of the enzyme was essentially identical through the species of the animals but the in... | [
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PMID:20318 | Experimental renal papillary necrosis. Effects of beta adrenergic receptor blockade, heparin and hydrocortisone. | Renal papillary necrosis was induced in rats by daily subcutaneous injection of 15 mg 2-bromoethylamine hydrobromide (BEA) per 100 g of body weight for 2 successive days. This dose was 50% higher than that reported previously. Beta adrenergic receptor blockade with oxyprenolol did not influence the kidney damage. The a... | Experimental renal papillary necrosis. Effects of beta adrenergic receptor blockade, heparin and hydrocortisone. Renal papillary necrosis was induced in rats by daily subcutaneous injection of 15 mg 2-bromoethylamine hydrobromide (BEA) per 100 g of body weight for 2 successive days. This dose was 50% higher than that r... | [
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PMID:20319 | Hypoxia in fibroblast cultures. 3. 35S-sulfate incorporation into acid mucopolysaccharides as influenced by 5% O2 hypoxia with simultaneous changes in the lactate-and H-ion concentrations. | The influence of 5% O2 hypoxia on the 35S-sulfate incorporation into different mucopolysaccharide fractions was studied in monolayer cultures of embryonic rat fibroblasts before reaching the stationary phase. Besides the O2 concentration also the pH value and lactate concentration were varied in the experiments. The re... | Hypoxia in fibroblast cultures. 3. 35S-sulfate incorporation into acid mucopolysaccharides as influenced by 5% O2 hypoxia with simultaneous changes in the lactate-and H-ion concentrations. The influence of 5% O2 hypoxia on the 35S-sulfate incorporation into different mucopolysaccharide fractions was studied in monolaye... | [
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PMID:20320 | Hypoxia in fibroblast cultures. 4. Cell density, glucose utilization and acid mucopolysaccharides in 1% O2 concentration. | Secondary cultures of embryonic rat fibroblasts incubated in Eagle medium with Hepes buffer at pH 6.6 and 7.4 were maintained in hypoxia of 1% O2 for 48 hours. The acid mucopolysaccharides were isolated from medium plus cells and fractionated on cellulose microcolumns according to SVEJCAR and ROBERTSON (1967). Glucose ... | Hypoxia in fibroblast cultures. 4. Cell density, glucose utilization and acid mucopolysaccharides in 1% O2 concentration. Secondary cultures of embryonic rat fibroblasts incubated in Eagle medium with Hepes buffer at pH 6.6 and 7.4 were maintained in hypoxia of 1% O2 for 48 hours. The acid mucopolysaccharides were isol... | [
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PMID:20321 | The problem of sea urchin egg fertilization and its implications for biological studies. | The analysis of sea urchin egg fertilization shows that several phenomena common to other biological systems are involved: cell recognition, cell fusion, exocytosis and initiation of mitotic activity. Both the role of calcium ions in cell fusion and exocytosis and the function of the cell surface in the initiation of m... | The problem of sea urchin egg fertilization and its implications for biological studies. The analysis of sea urchin egg fertilization shows that several phenomena common to other biological systems are involved: cell recognition, cell fusion, exocytosis and initiation of mitotic activity. Both the role of calcium ions ... | [
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PMID:20323 | Hydrogen peroxide generation in Trypanosoma cruzi. | Homogenates from T. cruzi epimastigotes produced 3.4 pmoles H2O2/min 10(6) cells, as detected by the cytochrome c peroxidase assay. Addition of NADH or NADPH increased H2O2 production by a factor of 3 and 5, respectively. When supplemented with NADH and NADPH, the mitochondrial, microsomal and supernatant fractions pro... | Hydrogen peroxide generation in Trypanosoma cruzi. Homogenates from T. cruzi epimastigotes produced 3.4 pmoles H2O2/min 10(6) cells, as detected by the cytochrome c peroxidase assay. Addition of NADH or NADPH increased H2O2 production by a factor of 3 and 5, respectively. When supplemented with NADH and NADPH, the mito... | [
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PMID:20324 | [Effect of several neuroleptic, adreno-, sympatho- and cholinolytic substances on the development of experimental cerebral edema induced by nicotine]. | The influence of some neuroleptic, adreno, sympatho- and cholinolytic substances on the development of experimental brain edema induced with nicotine was studied in tests conducted on rats. It was ascertained that marked antiedemic properties display drugs blocking the alpha-adrenoreceptors (phentolamine, dopegit), the... | [Effect of several neuroleptic, adreno-, sympatho- and cholinolytic substances on the development of experimental cerebral edema induced by nicotine]. The influence of some neuroleptic, adreno, sympatho- and cholinolytic substances on the development of experimental brain edema induced with nicotine was studied in test... | [
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PMID:20325 | [Pharmacologic study of clofelin]. | Clofelin--2-(2,6-dichlorphenylamino)-2-imidasoline, an analoque of foreign-made clonidine (St155, catapresan, hemiton, etc) was subjected to a pharmacological study. In animals the drug produces first a rise and then protracted fall of the arterial pressure, reduces the frequency of cardiac contractions, lessens reflex... | [Pharmacologic study of clofelin]. Clofelin--2-(2,6-dichlorphenylamino)-2-imidasoline, an analoque of foreign-made clonidine (St155, catapresan, hemiton, etc) was subjected to a pharmacological study. In animals the drug produces first a rise and then protracted fall of the arterial pressure, reduces the frequency of c... | [
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PMID:20326 | [Mechanism of the antianginal action of oxyfedrine]. | Oxyphedrine was found to depress the tonicity of coronary vessels, increase the coronary circulation volume, the oxygen uptake by the heart and also to exert a positive inoand chronotropic action in test with urethan and cloralose anesthetized cats. These effects were caused by stimulation of the beta-adrenoreceptors o... | [Mechanism of the antianginal action of oxyfedrine]. Oxyphedrine was found to depress the tonicity of coronary vessels, increase the coronary circulation volume, the oxygen uptake by the heart and also to exert a positive inoand chronotropic action in test with urethan and cloralose anesthetized cats. These effects wer... | [
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PMID:20330 | Peinamine, a new bisbenzylisoquinoline alkaloid from arrow tips (pei-namô) of the Upper Orinoco. | A new bisbenzylisoquinoline alkaloid has been isolated from a curare of the Upper Orinoco region. The curare is made by Indians from an unknown plant (probably Menispermacea) and is kept on arrow tips. The structure of the alkaloid, named peinamine, has been elucidated on the basis of its chemical reactions and spectro... | Peinamine, a new bisbenzylisoquinoline alkaloid from arrow tips (pei-namô) of the Upper Orinoco. A new bisbenzylisoquinoline alkaloid has been isolated from a curare of the Upper Orinoco region. The curare is made by Indians from an unknown plant (probably Menispermacea) and is kept on arrow tips. The structure of the ... | [
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PMID:20327 | [Study of phenothiazine binding to membranes by the fluorescent probe method]. | Binding of chlorpromazine, etaperazine and triftazine with model phospholipid membranes was investigated by using a 3-methoxybenzanthrone (MBA) fluorescent probe. All these preparations interacted with the membranes, this being registered by the MBA fluorescence extinction. The phenothiazine molecules bound with the me... | [Study of phenothiazine binding to membranes by the fluorescent probe method]. Binding of chlorpromazine, etaperazine and triftazine with model phospholipid membranes was investigated by using a 3-methoxybenzanthrone (MBA) fluorescent probe. All these preparations interacted with the membranes, this being registered by... | [
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PMID:20331 | Effect of dibenzepin hydrochloride on the oxygen, glucose uptake and oxidative phosphorylation of rat brain in vitro. | Dibenzepin hydrochloride (10(-3) M) decreases the oxygen uptake of whole brain homogenates and uncouples brain mitochondrial oxidative phosphorylation. At the same concentration this drug inhibits oxygen uptake of rat brain slices when calcium is absent in the incubation or then contains an excess of potassium. | Effect of dibenzepin hydrochloride on the oxygen, glucose uptake and oxidative phosphorylation of rat brain in vitro. Dibenzepin hydrochloride (10(-3) M) decreases the oxygen uptake of whole brain homogenates and uncouples brain mitochondrial oxidative phosphorylation. At the same concentration this drug inhibits oxyge... | [
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PMID:20328 | [Long-term experiments on toleration of the ganglionic blockader hygronium]. | In tests set up on albino rats, guinea pigs and rabbits the chronic toxicity of hygronium--a new original ganglion blocking agent of a short-term action was studied. With its daily intraperitoneal administration for 14 days in doses of 10 and 40 mg/kg (1/10 and 1/3 DL50) the drug produces in some of the animals, chiefl... | [Long-term experiments on toleration of the ganglionic blockader hygronium]. In tests set up on albino rats, guinea pigs and rabbits the chronic toxicity of hygronium--a new original ganglion blocking agent of a short-term action was studied. With its daily intraperitoneal administration for 14 days in doses of 10 and ... | [
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PMID:20329 | [Effect of contrast media on the rate of oxidation of NADP.H and NAD.H by rat liver microsomes]. | The effect of roentgen-contrast media on the activity of the NADP'N and NAD'N-dependent electron-transport chains of the rats liver microsomes was studied. Bilignost, cardiotrast, triiotrast and triombrin are shown to lower the rate of the NADP'N oxidation by the rats' liver microsomes and have no effect (except for tr... | [Effect of contrast media on the rate of oxidation of NADP.H and NAD.H by rat liver microsomes]. The effect of roentgen-contrast media on the activity of the NADP'N and NAD'N-dependent electron-transport chains of the rats liver microsomes was studied. Bilignost, cardiotrast, triiotrast and triombrin are shown to lower... | [
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PMID:20337 | Excretion in insects: function of gut and rectum in concentrating and diluting the urine. | The diverse excretory systems of insects exhibit several features that appear unusual when comparisons are made with the mammalian kidney. Secretion by the Malpighian tubules of a fluid that is unlike the blood in composition, substitutes for glomerular filtration. Various reabsorptive functions, such as volume reducti... | Excretion in insects: function of gut and rectum in concentrating and diluting the urine. The diverse excretory systems of insects exhibit several features that appear unusual when comparisons are made with the mammalian kidney. Secretion by the Malpighian tubules of a fluid that is unlike the blood in composition, sub... | [
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PMID:20338 | The frequency of Y chromatin-positive spermatozoa during in vitro penetration tests under various experimental conditions. | Complete analysis were carried out on the semen of 55 subjects. The semen was then investigated by using the fluorescent microscopic technique to determine the percentage proportion of Y chromatin-bearing spermatozoa. After staining with quinacrine mustard, an average Y chromatin frequency of 48.2% +/- 2.9% was recorde... | The frequency of Y chromatin-positive spermatozoa during in vitro penetration tests under various experimental conditions. Complete analysis were carried out on the semen of 55 subjects. The semen was then investigated by using the fluorescent microscopic technique to determine the percentage proportion of Y chromatin-... | [
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PMID:20339 | Xenogeneic neoplastic transplants in adult rats pretreated with thymic foetal extracts. | The paper deals with the effect of thymic extracts from young foetal pigs and calves on the development of tumour xenografts, i.e. mouse ascites Ehrlich carcinoma and hamster malignant melanoma, in rats. The results of experiments showed that Ehrlich carcinoma developed in 4 out of 72 animals, i.e., in those animals wh... | Xenogeneic neoplastic transplants in adult rats pretreated with thymic foetal extracts. The paper deals with the effect of thymic extracts from young foetal pigs and calves on the development of tumour xenografts, i.e. mouse ascites Ehrlich carcinoma and hamster malignant melanoma, in rats. The results of experiments s... | [
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PMID:20342 | Influence of steroid structure in relation to liver metabolism during endotoxin lethality in mice. | The influence of a number of steroid molecules on hepatic metabolism was determined in relationship to their ability to alter endotoxin lethality in intact mice. Progesterone, testosterone, estradiol, pregnenolone-16-alpha-carbonitrile and spironolactone did not alter endotoxin lethality, liver glycogen or tryptophan p... | Influence of steroid structure in relation to liver metabolism during endotoxin lethality in mice. The influence of a number of steroid molecules on hepatic metabolism was determined in relationship to their ability to alter endotoxin lethality in intact mice. Progesterone, testosterone, estradiol, pregnenolone-16-alph... | [
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PMID:20343 | A comparison of D & C and vacuum aspiration for performing first trimester abortion. | In a study of techniques for first trimester abortion conducted at Kandang Kerbau Hospital in Singapore from September 1973 to April 1975, 420 physically healthy gravidas were randomly assigned to one of two treatment groups either by D & C or vacuum aspiration. Half of the patients in each group were 6 to 10 menst... | A comparison of D & C and vacuum aspiration for performing first trimester abortion. In a study of techniques for first trimester abortion conducted at Kandang Kerbau Hospital in Singapore from September 1973 to April 1975, 420 physically healthy gravidas were randomly assigned to one of two treatment groups either... | [
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PMID:20345 | Female sterilization using the tubal ring. | The surgical and early postoperative complications were evaluated in a study of 221 laparoscopies and 29 minilaparotomies in which tubal occlusions were performed with the application of tubal rings. For either procedure, complications were infrequent. None of the procedures had to be completed by another technique of ... | Female sterilization using the tubal ring. The surgical and early postoperative complications were evaluated in a study of 221 laparoscopies and 29 minilaparotomies in which tubal occlusions were performed with the application of tubal rings. For either procedure, complications were infrequent. None of the procedures h... | [
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PMID:20346 | Contraceptive efficacy of two different metals using a modified seven vector. | Early studies with copper suggested that few improvements in overall IUD performance could be obtained with the use of more than 200 mm2 of copper. Thus, the use of other metals paired with copper has been investigated. This report of two studies of the small Copper-7, one with zinc and the other with copper alone, sug... | Contraceptive efficacy of two different metals using a modified seven vector. Early studies with copper suggested that few improvements in overall IUD performance could be obtained with the use of more than 200 mm2 of copper. Thus, the use of other metals paired with copper has been investigated. This report of two stu... | [
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PMID:20347 | The clinical efficacy of the repeated transcervical instillation of quinacrine for female sterilization. | The safety and efficacy of the repeated transcervical instillation of quinacrine hydrochloride in a suspension of 5 ml of 2% Xylocaine was evaluated in 200 patients. All instillation procedures were performed during the proliferative phase of the menstrual cycle: the second instillation was made in the first menstrual ... | The clinical efficacy of the repeated transcervical instillation of quinacrine for female sterilization. The safety and efficacy of the repeated transcervical instillation of quinacrine hydrochloride in a suspension of 5 ml of 2% Xylocaine was evaluated in 200 patients. All instillation procedures were performed during... | [
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PMID:20348 | Fetal heart rate response to maternal hypotension during amniocentesis. | Ten cases of severe fetal heart rate deceleration following maternal vaso-vagal reflex during amniocentesis were presented. The good outcome of these fetuses suggests that this transient phenomenon has no adverse effect on the fetus. This type of FHR response should be recognized so as to avoid unnecessary Caesarian se... | Fetal heart rate response to maternal hypotension during amniocentesis. Ten cases of severe fetal heart rate deceleration following maternal vaso-vagal reflex during amniocentesis were presented. The good outcome of these fetuses suggests that this transient phenomenon has no adverse effect on the fetus. This type of F... | [
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PMID:20349 | Non-acceptance of puerperal sterilization. A study. | This is an analysis of reasons given by 800 women who were hospitalized for deliveries at the Government Kilpauk Medical College Hospital in Madras for refusing puerperal sterilization. The cases are analysed by socio-demographic characteristics, age and parity. The most common reasons given were "inability to do manua... | Non-acceptance of puerperal sterilization. A study. This is an analysis of reasons given by 800 women who were hospitalized for deliveries at the Government Kilpauk Medical College Hospital in Madras for refusing puerperal sterilization. The cases are analysed by socio-demographic characteristics, age and parity. The m... | [
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PMID:20350 | Uterine rupture and vacuum extraction. | Eleven uterine ruptures occurred among 11415 vacuum extractions, and these cases are analysed. Cervical tears extending into the lower uterine segment or into the broad ligament occurred in 4 cases, lower segment tears in 6 and a fundal tear in 1. One patient died of respiratory failure. The possible cause of uterine r... | Uterine rupture and vacuum extraction. Eleven uterine ruptures occurred among 11415 vacuum extractions, and these cases are analysed. Cervical tears extending into the lower uterine segment or into the broad ligament occurred in 4 cases, lower segment tears in 6 and a fundal tear in 1. One patient died of respiratory f... | [
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PMID:20351 | The use of the ultrasonic echo technique in examining the normal and pathological involution in the puerperium. | The involution of the uterus in the puerperium was investigated after 50 normal, and 15 pathological pregnancies. By means of daily performed ultrasonic examinations the size and location of the uterus can be well determined, and the pace of the involution as well as the existing subinvolution can be diagnosed. It was ... | The use of the ultrasonic echo technique in examining the normal and pathological involution in the puerperium. The involution of the uterus in the puerperium was investigated after 50 normal, and 15 pathological pregnancies. By means of daily performed ultrasonic examinations the size and location of the uterus can be... | [
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PMID:20352 | Induced illegal abortions in Benin City, Nigeria. | Abortions of all types constitute about 25% of our gynaecological admissions. In 8 months period between July 1974 and February 1975, 59 patients admitted into our unit with confirmed illegally induced abortions were studied personally by the author. It was found that most patients with this type of abortion were singl... | Induced illegal abortions in Benin City, Nigeria. Abortions of all types constitute about 25% of our gynaecological admissions. In 8 months period between July 1974 and February 1975, 59 patients admitted into our unit with confirmed illegally induced abortions were studied personally by the author. It was found that m... | [
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PMID:20353 | Human chorionic somatomammotropin in normal pregnancy in an Asian population. | Serial Human Chorionic Somatomammotropin (HCS) levels were measured during the last 10 weeks of pregnancy in 57 patients (375 samples). There was a gradual rise from 5.6 microgram/ml at the 30th week to microgram/ml at the 40th week, with a plateau during the last 4 weeks of pregnancy. No correlation could be found of ... | Human chorionic somatomammotropin in normal pregnancy in an Asian population. Serial Human Chorionic Somatomammotropin (HCS) levels were measured during the last 10 weeks of pregnancy in 57 patients (375 samples). There was a gradual rise from 5.6 microgram/ml at the 30th week to microgram/ml at the 40th week, with a p... | [
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PMID:20354 | Fetal heart rate and fetal movements. | Fetal Heart Rate (F.H.R.) in association with fetal movement was evaluated in 141 normal and pathological pregnancies. In the normal cases only 31% showed an acceleration of F.H.R. in association with fetal movement. The majority of the normal cases, 62%, did not demonstrate changes in F.H.R. in association with fetal ... | Fetal heart rate and fetal movements. Fetal Heart Rate (F.H.R.) in association with fetal movement was evaluated in 141 normal and pathological pregnancies. In the normal cases only 31% showed an acceleration of F.H.R. in association with fetal movement. The majority of the normal cases, 62%, did not demonstrate change... | [
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PMID:20355 | Iron deficiency anaemia in a rural area in Nigeria. Evaluation of diagnosis and treatment with imferon. | In 32 pregnant women with packed cell volume (PCV) of 30% or less (Hb 10 g/100 ml or below), iron depletion was present in 87.5% and megaloblastic changes in 84.5% as judged by bone marrow findings. Parenteral iron gave statistically significant rise in the PCV (p less than 0.001) in those who received iron therapy com... | Iron deficiency anaemia in a rural area in Nigeria. Evaluation of diagnosis and treatment with imferon. In 32 pregnant women with packed cell volume (PCV) of 30% or less (Hb 10 g/100 ml or below), iron depletion was present in 87.5% and megaloblastic changes in 84.5% as judged by bone marrow findings. Parenteral iron g... | [
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PMID:20356 | Acute carcinomatous myopathy associated with ovarian carcinoma. | A case of acute, fatal, rapidly progressive pure myopathy associated with ovarian carcinoma is described. The first symptoms of carcinomatous myopathy occurred 8 months after surgical treatment and combined chemotherapy. The patient died 2 months after onset of myopathy. The clinical and pathologic findings of the myop... | Acute carcinomatous myopathy associated with ovarian carcinoma. A case of acute, fatal, rapidly progressive pure myopathy associated with ovarian carcinoma is described. The first symptoms of carcinomatous myopathy occurred 8 months after surgical treatment and combined chemotherapy. The patient died 2 months after ons... | [
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PMID:20357 | The effect of miconazole on vulvo-vaginal candidosis in pregnant and non-pregnant women and their partners. | Miconazole cream (Daktar vaginal cream 2%, Leo) given for 12-14 days was tested in 85 subjects with positive vaginal candida culture. Eighty percent of the patients were pregnant. Healing was observed in 93% of the cases. Treatment of the sexual partner did not influence the therapeutic effect. | The effect of miconazole on vulvo-vaginal candidosis in pregnant and non-pregnant women and their partners. Miconazole cream (Daktar vaginal cream 2%, Leo) given for 12-14 days was tested in 85 subjects with positive vaginal candida culture. Eighty percent of the patients were pregnant. Healing was observed in 93% of t... | [
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PMID:20358 | Use of prostaglandin F2alpha in the management of missed abortion. | Intravenous infusion of PG F2alpha was used to induce uterine contractions in 9 cases of missed abortions. Except for one case which was an extra uterine pregnancy, all of the patients aborted successfully. There were no major complications. The use of PG is suggested as a method of choice in the management of missed a... | Use of prostaglandin F2alpha in the management of missed abortion. Intravenous infusion of PG F2alpha was used to induce uterine contractions in 9 cases of missed abortions. Except for one case which was an extra uterine pregnancy, all of the patients aborted successfully. There were no major complications. The use of ... | [
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PMID:20359 | Transplacental passage of diazepam following intravenous injection immediately prior to operative vaginal delivery. | The early phase of diaplacental transfer of diazepam was studied in 39 women given the drug as a basic anaesthetic for operative vaginal delivery indicated by prolonged second stage of labour (9 cases), breech delivery (19 cases) and intrauterine hypoxia (11 cases). A total dose of 30 mg diazepam (Valium Roche) was inj... | Transplacental passage of diazepam following intravenous injection immediately prior to operative vaginal delivery. The early phase of diaplacental transfer of diazepam was studied in 39 women given the drug as a basic anaesthetic for operative vaginal delivery indicated by prolonged second stage of labour (9 cases), b... | [
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PMID:20360 | Serum naphythylamidase isoenzymes during hormonal treatment. Electrophoretic and quantitative studies. | Alterations in serum naphthylamidase isoenzymes were studied by electrophoretic and quantitative methods in women treated with oral contraceptives and women treated with naturally occurring conjugated estrogens for climacteric symptoms. In women treated with oral contraceptives the appearance of extra isoenzyme compone... | Serum naphythylamidase isoenzymes during hormonal treatment. Electrophoretic and quantitative studies. Alterations in serum naphthylamidase isoenzymes were studied by electrophoretic and quantitative methods in women treated with oral contraceptives and women treated with naturally occurring conjugated estrogens for cl... | [
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PMID:20361 | Homotransplantation of the amniotic membrane for the treatment of congenital absence of the vagina. | Homotransplantation of the amniotic membrane for the treatment of congenital absence of the vagina is described. Two years' follow-up with biopsy specimens revealed that amniotic membrane cells divide mitotically and activate vaginal epithelial proliferation of the squamous type and which respond to exogenous estrogens... | Homotransplantation of the amniotic membrane for the treatment of congenital absence of the vagina. Homotransplantation of the amniotic membrane for the treatment of congenital absence of the vagina is described. Two years' follow-up with biopsy specimens revealed that amniotic membrane cells divide mitotically and act... | [
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PMID:20362 | Perforation of the uterus by copper-IUDs. | In about 5 000 copper-IUD insertions 5 cases of perforation were found. One woman had 2 devices inserted, both of them perforated. Copper-T was responsible for 3 corporal perforations and copper-7 for 2 corporal and 1 cervical perforation. | Perforation of the uterus by copper-IUDs. In about 5 000 copper-IUD insertions 5 cases of perforation were found. One woman had 2 devices inserted, both of them perforated. Copper-T was responsible for 3 corporal perforations and copper-7 for 2 corporal and 1 cervical perforation. | [
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PMID:20363 | Twins, immuno-incompatibility and pre-eclampsia. | A survey of 417 twin pregnancies is reported. Examination of the data revealed no difference in the incidence of pre-eclampsia referable to the zygosity of the twins. The findings thus lends no support to the hypothesis that immuno-incompatibility contributes to the aetiology of pre-eclampsia. | Twins, immuno-incompatibility and pre-eclampsia. A survey of 417 twin pregnancies is reported. Examination of the data revealed no difference in the incidence of pre-eclampsia referable to the zygosity of the twins. The findings thus lends no support to the hypothesis that immuno-incompatibility contributes to the aeti... | [
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PMID:20366 | Calcified myoma of utero-ovarian ligament in a pubertal girl. | A calcified myoma of the utero-ovarian ligament was encountered in a 13-year-old girl. The age of the patient and the unusual location prompt this report of a possibly unique entity, and the pertinent literature is cited. | Calcified myoma of utero-ovarian ligament in a pubertal girl. A calcified myoma of the utero-ovarian ligament was encountered in a 13-year-old girl. The age of the patient and the unusual location prompt this report of a possibly unique entity, and the pertinent literature is cited. | [
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PMID:20384 | An enzymatic time/temperature device for monitoring the handling of perishable commodities. | Kockums Chemical AB, Malmö, Sweden, has developed a biochemical indicator, the i-point TTM (Time-Temperature Monitor). The indicator responds to temperature in much the same way as perishable commodities in which loss of quality is directly related to the combined effects of the degree and the duration of storage tempe... | An enzymatic time/temperature device for monitoring the handling of perishable commodities. Kockums Chemical AB, Malmö, Sweden, has developed a biochemical indicator, the i-point TTM (Time-Temperature Monitor). The indicator responds to temperature in much the same way as perishable commodities in which loss of quality... | [
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PMID:20385 | Interrelationships between histamine, prostaglandins, and cyclic AMP in gastric secretion: a hypothesis. | Based on the current experimental evidence, a model is proposed for mutual interactions of histamine, prostaglandins, and cyclic AMP in regulation of gastric secretion and pathogenesis of peptic ulcer. Histamine acting on H2-receptor-associated adenylate cyclase stimulates cyclic AMP formation and consequently secretio... | Interrelationships between histamine, prostaglandins, and cyclic AMP in gastric secretion: a hypothesis. Based on the current experimental evidence, a model is proposed for mutual interactions of histamine, prostaglandins, and cyclic AMP in regulation of gastric secretion and pathogenesis of peptic ulcer. Histamine act... | [
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PMID:20386 | Gastric adenosine triphosphatases: a review of their possible role in HCl secretion. | In the search for gastric ATPases that might be related to the mechanism of HCl secretion, an interesting and rather unique K+-stimulated ATPase has been discovered. This enzyme is isolated from oxyntic cells and has been associated with the apical plasma membrane and/or tubulovesicular system. Membrane vesicles contai... | Gastric adenosine triphosphatases: a review of their possible role in HCl secretion. In the search for gastric ATPases that might be related to the mechanism of HCl secretion, an interesting and rather unique K+-stimulated ATPase has been discovered. This enzyme is isolated from oxyntic cells and has been associated wi... | [
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PMID:20387 | Electrical events during activation and inhibition of gastric HCl secretion. | A brief review of some of the relationships between the secretory rate and the electrophysiological characteristics of the canine and frog gastric mucosa is presented. There is no simple relationship between the potential difference (PD) and H+ rate in the in vitro frog gastric mucosa with standard Cl- bathing solution... | Electrical events during activation and inhibition of gastric HCl secretion. A brief review of some of the relationships between the secretory rate and the electrophysiological characteristics of the canine and frog gastric mucosa is presented. There is no simple relationship between the potential difference (PD) and H... | [
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PMID:20392 | [Drug effects on blood pressure and heart rate in unanesthetized animals. (1). Effects of beta-blocking agents (author's transl)]. | beta-Blocking actions of orally administered Kö 1400 and tiprenolol, new beta-blocking agents, were studied in unanesthetized rats and dogs, using a fall of blood pressure and an increase in heart rate produced by isoproterenol as a measure of beta-receptor activation. Blood pressure was recorded from the aorta of the ... | [Drug effects on blood pressure and heart rate in unanesthetized animals. (1). Effects of beta-blocking agents (author's transl)]. beta-Blocking actions of orally administered Kö 1400 and tiprenolol, new beta-blocking agents, were studied in unanesthetized rats and dogs, using a fall of blood pressure and an increase i... | [
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PMID:20393 | [Effects of a new adrenergic beta-blocking agent, Kö 1400 on the canine heart-lung preparation supported by a donor dog and the perfused hindlimb preparation of the dog (author's transl)]. | Using the canine heart-lung preparation supported by a donor, effects of a new adrenergic beta-blocking agent, dl-1-(tert. butylamino)-3-[ (2-propinyloxy) phenoxy]-2-propanol hydrochloride (Kö 1400), on cardiac function, myocardial metabolism, and coronary circulation were studied and compared with those of propranolol... | [Effects of a new adrenergic beta-blocking agent, Kö 1400 on the canine heart-lung preparation supported by a donor dog and the perfused hindlimb preparation of the dog (author's transl)]. Using the canine heart-lung preparation supported by a donor, effects of a new adrenergic beta-blocking agent, dl-1-(tert. butylami... | [
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PMID:20395 | [Studies on gamma-oryzanol. III. Influence of gamma-oryzanol on circadian rhythms of serum gastrin, 11-OHCS and gastric secretion in rats (author's transl)]. | In the course of investigations of gamma-oryzanol in rats, circadian rhythms in antiulcerogenic action on gamma-oryzanol were observed. Circadian rhythms in several other parameters were investigated and correlated with the antiulcerogenic action. Pretreatment of rats with gamma-oryzanol 100 mg/kg, s.c., for 5 days ten... | [Studies on gamma-oryzanol. III. Influence of gamma-oryzanol on circadian rhythms of serum gastrin, 11-OHCS and gastric secretion in rats (author's transl)]. In the course of investigations of gamma-oryzanol in rats, circadian rhythms in antiulcerogenic action on gamma-oryzanol were observed. Circadian rhythms in sever... | [
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PMID:20396 | [Comparative study between dobutamine and other catecholamines in their effects on the cardiac contraction and rhythm (author's transl)]. | Dobutamine (DOB) showed a dose dependent positive introphic effect on the right ventricular muscle of vagotomized Beagle dogs under pentobarbital anesthesia. Positve inotropic effects of other catecholamines (CA) decreased in the following order; isoproterenol (Iso, 115) greater than norepinephrine (NE, 15) greater tha... | [Comparative study between dobutamine and other catecholamines in their effects on the cardiac contraction and rhythm (author's transl)]. Dobutamine (DOB) showed a dose dependent positive introphic effect on the right ventricular muscle of vagotomized Beagle dogs under pentobarbital anesthesia. Positve inotropic effect... | [
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PMID:20398 | [Clinical picture of lactate acidosis. 4: Clinical significance of lactate acidosis]. | The diagnosis of lactate acidosis is complicated by the fact that lactate determination is not a routine method in clinical chemistry. In fact, lactate analysis is performed only in special laboratories. Even in greater clinics this method is not routinely performed in differential diagnosis of acidotic states. Various... | [Clinical picture of lactate acidosis. 4: Clinical significance of lactate acidosis]. The diagnosis of lactate acidosis is complicated by the fact that lactate determination is not a routine method in clinical chemistry. In fact, lactate analysis is performed only in special laboratories. Even in greater clinics this m... | [
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PMID:20399 | Pathogenesis of cryptorchidism. | Cryptorchidism was induced experimentally by treating pregnant mice on the 14th day of pregnancy with 5 mg estrogen. Testes from cryptorchid and control newborn and adult mice were investigated with radioimmunoassay and electron microscopy. It was concluded that a normal Leydig cell function plays a decisive role in te... | Pathogenesis of cryptorchidism. Cryptorchidism was induced experimentally by treating pregnant mice on the 14th day of pregnancy with 5 mg estrogen. Testes from cryptorchid and control newborn and adult mice were investigated with radioimmunoassay and electron microscopy. It was concluded that a normal Leydig cell func... | [
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PMID:20400 | The use of luteinizing hormone-releasing hormone in pediatric patients. | Luteinizing hormone-releasing hormone (LH-RH), first synthetized in 1971; became soon available for clinical purposes and was immediately used as a diagnostic tool in children and adolescents as well as in adults. The first results of the LH-RH test in pediatrics were reported in 1972 and soon after discussed in meetin... | The use of luteinizing hormone-releasing hormone in pediatric patients. Luteinizing hormone-releasing hormone (LH-RH), first synthetized in 1971; became soon available for clinical purposes and was immediately used as a diagnostic tool in children and adolescents as well as in adults. The first results of the LH-RH tes... | [
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PMID:20403 | Differences in mouse interferons according to cell source and mode of induction. | Mouse interferon induced by ultraviolet-irradiated Newcastle disease virus or polyriboinosinic-polyribocytidylic acid in T lymphocytes, B lymphocytes, macrophages, and primary mouse embryonic cell culture was studied. Irrespective of the inducer, interferons produced by T or B lymphocytes were relatively heat stable an... | Differences in mouse interferons according to cell source and mode of induction. Mouse interferon induced by ultraviolet-irradiated Newcastle disease virus or polyriboinosinic-polyribocytidylic acid in T lymphocytes, B lymphocytes, macrophages, and primary mouse embryonic cell culture was studied. Irrespective of the i... | [
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PMID:20404 | Repression of heat-stable enterotoxin synthesis in enterotoxigenic Escherichia coli. | Five different carbon sources were examined for their ability to control synthesis of heat-stable enterotoxin (ST) by enterotoxigenic (ENT+) Escherichia coli grown in either a defined medium containing four amino acids or a minimal salts medium. No ST activity was observed when D-glucose, D-gluconate, and L-arabinose w... | Repression of heat-stable enterotoxin synthesis in enterotoxigenic Escherichia coli. Five different carbon sources were examined for their ability to control synthesis of heat-stable enterotoxin (ST) by enterotoxigenic (ENT+) Escherichia coli grown in either a defined medium containing four amino acids or a minimal sal... | [
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PMID:20405 | Complement levels in pneumococcal pneumonia. | Levels of complement proteins and functional activity of the alternate complement pathway were assessed in 39 patients with pneumococcal pneumonia. Mean levels of C3 and properdin and the functional activity of the alternate pathway in acute sera were significantly (P less than 0.05) below normal, whereas levels of com... | Complement levels in pneumococcal pneumonia. Levels of complement proteins and functional activity of the alternate complement pathway were assessed in 39 patients with pneumococcal pneumonia. Mean levels of C3 and properdin and the functional activity of the alternate pathway in acute sera were significantly (P less t... | [
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PMID:20406 | Cell envelope of Neisseria gonorrhoeae: relationship between autolysis in buffer and the hydrolysis of peptidoglycan. | Neisseria gonorrhoeae readily underwent autolysis when suspended in N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer at alkaline pH values. Autolysis was inhibited by the addition of Mg2+ or other divalent cations. Autolysis was also suppressed at acid pH (pH 6.0). Suspension of cells in buffer was ac... | Cell envelope of Neisseria gonorrhoeae: relationship between autolysis in buffer and the hydrolysis of peptidoglycan. Neisseria gonorrhoeae readily underwent autolysis when suspended in N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) buffer at alkaline pH values. Autolysis was inhibited by the addition of M... | [
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PMID:20407 | Delayed hypersensitivity in tumor-bearing mice. In vitro activation of "eclipsed" spleen cells. | At various stages during the progressive growth of a transplanted sarcoma in BALB/c mice, the delayed hypersensitivity response to tumor antigen was determined using the food-pad swelling test (FPS) and the leukocyte migration inhibition assay (LMI). A close correlation was observed between the in vivo and in vitro ass... | Delayed hypersensitivity in tumor-bearing mice. In vitro activation of "eclipsed" spleen cells. At various stages during the progressive growth of a transplanted sarcoma in BALB/c mice, the delayed hypersensitivity response to tumor antigen was determined using the food-pad swelling test (FPS) and the leukocyte migrati... | [
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PMID:20409 | Lack of association of the development of anti-sperm antibodies and other autoantibodies as a consequence of vasectomy. | The possibility of autoantibodies--other than sperm antibodies--developing as a consequence of vasectomy has been investigated in 255 volunteers. During the first year after vasectomy no obvious increase was observed in the occurrence of any of these antibodies (rheumatoid factor, antinuclear antibodies and antibodies ... | Lack of association of the development of anti-sperm antibodies and other autoantibodies as a consequence of vasectomy. The possibility of autoantibodies--other than sperm antibodies--developing as a consequence of vasectomy has been investigated in 255 volunteers. During the first year after vasectomy no obvious incre... | [
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PMID:20410 | Mixed lymphocyte culture responses in infertile couples. | Twenty couples suffering from unexplained infertility have been tested in mixed leucocyte culture to determine if the wives were preimmunized against their husbands. The wives were tested against their husbands and four unrelated persons, and thymidine incorporation was measured on the third and fifth days of culture. ... | Mixed lymphocyte culture responses in infertile couples. Twenty couples suffering from unexplained infertility have been tested in mixed leucocyte culture to determine if the wives were preimmunized against their husbands. The wives were tested against their husbands and four unrelated persons, and thymidine incorporat... | [
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PMID:20411 | A simplified determination of the urinary luteinizing hormone surge using the hemagglutination inhibition test. | The hemagglutination-inhibition test for urinary luteinizing hormone (LH) has been modified so that the test for LH surge can be assayed in 1 day. A first morning voided urine specimen submitted to the laboratory can be analyzed in 5 hours by ordinary bench procedures. This modification involves a 20-fold reduction, as... | A simplified determination of the urinary luteinizing hormone surge using the hemagglutination inhibition test. The hemagglutination-inhibition test for urinary luteinizing hormone (LH) has been modified so that the test for LH surge can be assayed in 1 day. A first morning voided urine specimen submitted to the labora... | [
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PMID:20412 | Hysteroscopic sterilization--a routine method? | Our intention was to sterilize 50 women by means of hysteroscopic sterilization, and we were unsuccessful in 60% of these cases. All these women ran the risk of another pregnancy with hysteroscopic sterilization alone. Therefore--in our opinion--the method of hysteroscopic sterilization by thermocoagulation of the intr... | Hysteroscopic sterilization--a routine method? Our intention was to sterilize 50 women by means of hysteroscopic sterilization, and we were unsuccessful in 60% of these cases. All these women ran the risk of another pregnancy with hysteroscopic sterilization alone. Therefore--in our opinion--the method of hysteroscopic... | [
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PMID:20408 | Fatty acid synthesizing enzyme activity of cultured Mycobacterium lepraemurium. | In comparing the specific activity of enzymes pertaining to the biosynthesis of fatty acids in crude extracts of cultivated M. lepraemurium and M. smegmatis, it was found that: 1. The activity of acetyl CoA carboxylase of the former organism was undetectable and that of de novo fatty acid synthetase was too weak to mea... | Fatty acid synthesizing enzyme activity of cultured Mycobacterium lepraemurium. In comparing the specific activity of enzymes pertaining to the biosynthesis of fatty acids in crude extracts of cultivated M. lepraemurium and M. smegmatis, it was found that: 1. The activity of acetyl CoA carboxylase of the former organis... | [
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PMID:20413 | The selection of Y-chromatin positive spermatozoa and subsequent in vitro penetration through cervical mucus. | We carried out a check of Ericsson's data on the possibility of separating out Y-spermatozoa and their selective enrichement. Layers of spermatozoa were laid on BSA media of various concentrations, filtered and the Y-chromatin proportion was determined. The method used was that of fluorochromation with quinacrine musta... | The selection of Y-chromatin positive spermatozoa and subsequent in vitro penetration through cervical mucus. We carried out a check of Ericsson's data on the possibility of separating out Y-spermatozoa and their selective enrichement. Layers of spermatozoa were laid on BSA media of various concentrations, filtered and... | [
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PMID:20414 | Etiology, clinical features and prognosis in secondary amenorrhea. | A clinical investigation of 356 patients with secondary amenorrhea revealed that 95% of patients with postpill amenorrhea and 56% of patients with anorexia nervosa recovered in 6 years. The corresponding recovery rates for patients with psychogenic amenorrhea and amenorrhea following self-induced weight loss were 72% f... | Etiology, clinical features and prognosis in secondary amenorrhea. A clinical investigation of 356 patients with secondary amenorrhea revealed that 95% of patients with postpill amenorrhea and 56% of patients with anorexia nervosa recovered in 6 years. The corresponding recovery rates for patients with psychogenic amen... | [
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PMID:20415 | Serum LH, FSH and testosterone response to the administration of a new LH-RH analog, D-Trp6-LH-RH, in normal men. | A long-acting superactive analog of LH-RH, D-Trp6-LH-RH was given to 23 normal men by several routes of administration (iv, im, sc, continuous infusion) and in increasing doses of 1 to 50 microgram. LH and FSH responses were obtained at doses as low as 2.5 microgram. The maximal absolute LH and FSH increment in respons... | Serum LH, FSH and testosterone response to the administration of a new LH-RH analog, D-Trp6-LH-RH, in normal men. A long-acting superactive analog of LH-RH, D-Trp6-LH-RH was given to 23 normal men by several routes of administration (iv, im, sc, continuous infusion) and in increasing doses of 1 to 50 microgram. LH and ... | [
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PMID:20416 | In vitro stimulation of human sperm motility by acetylcarnitine. | An increase in motility of ejaculated human spermatozoa was observed after the addition of acetylcaritine or carnitine. Similar results were also obtained in the diluted semen with 25--30% initial motilities. However, indirect evidence suggests that carnitine is converted to acetylcarnitine prior to its stimulatory act... | In vitro stimulation of human sperm motility by acetylcarnitine. An increase in motility of ejaculated human spermatozoa was observed after the addition of acetylcaritine or carnitine. Similar results were also obtained in the diluted semen with 25--30% initial motilities. However, indirect evidence suggests that carni... | [
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PMID:20417 | Osmolality and conductance of normal and abnormal human seminal plasma. | The possible correlation between two important general properties of seminal plasma, osmolality and conductance, and the presence of some pathological conditions of human semen, asthenospermia and oligoasthenospermia, have been studied. Osmolality was measured under carefully controlled conditions by the freezing point... | Osmolality and conductance of normal and abnormal human seminal plasma. The possible correlation between two important general properties of seminal plasma, osmolality and conductance, and the presence of some pathological conditions of human semen, asthenospermia and oligoasthenospermia, have been studied. Osmolality ... | [
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PMID:20418 | Uterine synechiae: variations in severity and some conditions which may be conducive to severe adhesions. | Considerable variability in the severity of Asherman's syndrome was observed, and a high incidence of other gynecological disorders was discovered, mainly by endoscopy, in patients with intrauterine synechiae. Fifty-seven cases were reviewed in an attempt (1) to discover factors contributing to severity, (2) to examine... | Uterine synechiae: variations in severity and some conditions which may be conducive to severe adhesions. Considerable variability in the severity of Asherman's syndrome was observed, and a high incidence of other gynecological disorders was discovered, mainly by endoscopy, in patients with intrauterine synechiae. Fift... | [
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PMID:20424 | Urinary excretion of uricine. | The excretion of uricine or yellowish-red pigment from uric acid stones and its binding by uric acid seems to affect the precipitation of uric acid. Uricine was determined by ion exchange chromatography followed by measurement of the alkaline fluorescence emission. The uricine urinary excretion and the uricine-uric aci... | Urinary excretion of uricine. The excretion of uricine or yellowish-red pigment from uric acid stones and its binding by uric acid seems to affect the precipitation of uric acid. Uricine was determined by ion exchange chromatography followed by measurement of the alkaline fluorescence emission. The uricine urinary excr... | [
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0.0328... |
PMID:20427 | Localization of histamine and histamine H2-receptor antagonists in the gastric mucosa. | Histamine stimulates acid secretion by the parietal cell and this secretion is inhibited by the histamine H2-receptor antagonists. Whole body autoradiography showed that radioactivity from 14C-histamine was localized in the artery walls of the stomach and in the muscularis mucosae, but that the level in the fundic muco... | Localization of histamine and histamine H2-receptor antagonists in the gastric mucosa. Histamine stimulates acid secretion by the parietal cell and this secretion is inhibited by the histamine H2-receptor antagonists. Whole body autoradiography showed that radioactivity from 14C-histamine was localized in the artery wa... | [
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PMID:20432 | Professional nursing in the Soviet Union. | Professional nurses in the Soviet Union are trained by physicians. Their professional training is planned to produce efficient, middle level, clinical workers. At all times nurses work under physician supervision. The Soviet nurse works in clinical facilities and with equipment that appears old-fashioned by American st... | Professional nursing in the Soviet Union. Professional nurses in the Soviet Union are trained by physicians. Their professional training is planned to produce efficient, middle level, clinical workers. At all times nurses work under physician supervision. The Soviet nurse works in clinical facilities and with equipment... | [
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0.017... |
PMID:20436 | Bacterial metabolism of anthracycline antibiotics. Steffimycinone and steffimycinol conversions. | Streptomyces nogalater, UC-2783, and Streptomyces peucetius var. caesius, IMRU-3920/UC-5633, catalyze ketonic carbonyl reduction of steffimycinone (1, Scheme 1). Using cell-free preparations of S. nogalater, the process of ketonic carbonyl reduction has been shown to be TPNH linked. The product, steffimycinol (2), is r... | Bacterial metabolism of anthracycline antibiotics. Steffimycinone and steffimycinol conversions. Streptomyces nogalater, UC-2783, and Streptomyces peucetius var. caesius, IMRU-3920/UC-5633, catalyze ketonic carbonyl reduction of steffimycinone (1, Scheme 1). Using cell-free preparations of S. nogalater, the process of ... | [
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PMID:20440 | Postcapillary changes in blood pH in vivo during carbonic anhydrase inhibition. | A rapidly responding stopped-flow glass pH electrode apparatus was used to investigate pH changes in blood in vivo after it exits from an exchange capillary. Arterial blood was drawn from anesthetized animals through the apparatus. Temperature and pH of the blood in the electrode chamber were continuously recorded, bot... | Postcapillary changes in blood pH in vivo during carbonic anhydrase inhibition. A rapidly responding stopped-flow glass pH electrode apparatus was used to investigate pH changes in blood in vivo after it exits from an exchange capillary. Arterial blood was drawn from anesthetized animals through the apparatus. Temperat... | [
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PMID:20441 | Cerebral glucose-6-phosphatase and the movement of 2-deoxy-D-glucose across cell membranes. | Glucose-6-phosphatase (glucose-6-phosphohydrolase and its associated phosphotransferase activities) was determined in brain tissue and in several preparations derived from brain tissue. These included purified capillaries and established cell lines of neuronal or glial origin. Since it has been suggested that glucose-6... | Cerebral glucose-6-phosphatase and the movement of 2-deoxy-D-glucose across cell membranes. Glucose-6-phosphatase (glucose-6-phosphohydrolase and its associated phosphotransferase activities) was determined in brain tissue and in several preparations derived from brain tissue. These included purified capillaries and es... | [
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0.023655498400330544,
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-... |
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