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PMID:14627 | Labetalol (AH5158), a competitive alpha- and beta-receptor blocking drug, in the management of hypertension. | The effects of intravenous labetalol, a drug with both alpha- and beta-adrenergic receptor blocking actions, have been studied in 20 severely hypertensive patients. There was a prompt and sustained fall in arterial pressure; severe hypotension did not occur in recumbent patients but postural hypotension was common. Pul... | Labetalol (AH5158), a competitive alpha- and beta-receptor blocking drug, in the management of hypertension. The effects of intravenous labetalol, a drug with both alpha- and beta-adrenergic receptor blocking actions, have been studied in 20 severely hypertensive patients. There was a prompt and sustained fall in arter... | [
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PMID:14630 | A comprehensive behaviour modification programme for the treatment of anorexia nervosa: results in six cases. | Behavioural analysis and psychodynamic hypotheses were used to develop a comprehensive behaviour modification programme for the treatment of primary anorexia nervosa. This was applied to six female patients and the results obtained are reported. | A comprehensive behaviour modification programme for the treatment of anorexia nervosa: results in six cases. Behavioural analysis and psychodynamic hypotheses were used to develop a comprehensive behaviour modification programme for the treatment of primary anorexia nervosa. This was applied to six female patients and... | [
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PMID:14645 | The relationship between chemical structure and basicity in some morpholine compounds. | The influence of various substituents upon the pKa values of a series of 2-phenylmorpholines is discussed. The results are interpreted in terms of the preferred conformations of the morpholine ring and the inductive effect of the various substituents. The lack of analgesic activity in these compounds could be attribute... | The relationship between chemical structure and basicity in some morpholine compounds. The influence of various substituents upon the pKa values of a series of 2-phenylmorpholines is discussed. The results are interpreted in terms of the preferred conformations of the morpholine ring and the inductive effect of the var... | [
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PMID:14646 | Hysteresis at an electrochemical membrane model and its possible role in circulation research. | On an artificial electrochemical membrane, properties of hysteresis are demonstrated by cyclic variation of pH. The properties are decreased by cAMP, theophylline, cysteine, heparin and increased by ADP. | Hysteresis at an electrochemical membrane model and its possible role in circulation research. On an artificial electrochemical membrane, properties of hysteresis are demonstrated by cyclic variation of pH. The properties are decreased by cAMP, theophylline, cysteine, heparin and increased by ADP. | [
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PMID:14647 | Physiological antagonism between PGE2, PGA1, PGF1-alpha and NADP, beta-NAD on isolated rabbit jejunum. | The antagonistic action between the prostaglandins PGE2, PGA1 and PGF1-alpha, on the one hand, and nicotinamide-adenine-dinucleotide-phosphate (NADP or TPN) and beta-nicotinamide-adenine-dinucleotide (beta-NAD or DPN) on the other, were experimentally studied on rabbit isolated jejunum. Our results support our previous... | Physiological antagonism between PGE2, PGA1, PGF1-alpha and NADP, beta-NAD on isolated rabbit jejunum. The antagonistic action between the prostaglandins PGE2, PGA1 and PGF1-alpha, on the one hand, and nicotinamide-adenine-dinucleotide-phosphate (NADP or TPN) and beta-nicotinamide-adenine-dinucleotide (beta-NAD or DPN)... | [
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PMID:14648 | An experimental and clinical pharmacological study of the influence of triamterene on the diuretic and saluretic properties of furosemide xantinol. | The diuretic and saluretic effect of furosemide xantinol administered alone and mixed with different amounts of 2,4,7-triamino-6-phenylpteridine (triamterene) have been investigated in rat and man. The presence of triamterene resulted in a dose dependent inhibition of furosemide xantinol induced diuresis in both specie... | An experimental and clinical pharmacological study of the influence of triamterene on the diuretic and saluretic properties of furosemide xantinol. The diuretic and saluretic effect of furosemide xantinol administered alone and mixed with different amounts of 2,4,7-triamino-6-phenylpteridine (triamterene) have been inv... | [
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PMID:14649 | Inhibitory effect of beta-adrenergic stimulants on the histamine reaction in human skin. I. Studies with fenoterol. | In 20 volunteers histamine inhibition by fenoterol (Th 1165 a) was studied. The wheal and erythema reaction caused by intracutaneous application of 5 mug histamine can be inhibited by applying fenoterol in doses from 100--400 mug in form of a metered aerosol on the skin 5 min before the injection of histamine. In this ... | Inhibitory effect of beta-adrenergic stimulants on the histamine reaction in human skin. I. Studies with fenoterol. In 20 volunteers histamine inhibition by fenoterol (Th 1165 a) was studied. The wheal and erythema reaction caused by intracutaneous application of 5 mug histamine can be inhibited by applying fenoterol i... | [
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PMID:14651 | [Double-blind clinical study of carpipramine/placebo (author's transl)]. | A statistically planned double blind cross-over test with the substances 1-[3-(10,11-dihydro-5H-dibenz[b;f]-azepin-5-yl)-propyl]-4-piperidino-piperidine-4-carboxamide dihydrochloride-monohydrate (carpipramine, BAY b 4343 b) and placebo (BAY b 4343 a) was carried out on 30 long-term hospitalized schizophrenic patients. ... | [Double-blind clinical study of carpipramine/placebo (author's transl)]. A statistically planned double blind cross-over test with the substances 1-[3-(10,11-dihydro-5H-dibenz[b;f]-azepin-5-yl)-propyl]-4-piperidino-piperidine-4-carboxamide dihydrochloride-monohydrate (carpipramine, BAY b 4343 b) and placebo (BAY b 4343... | [
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PMID:14652 | [Activity profile of carpipramine. Results of an open trial and a double-blind trial versus doxepin]. | During an uncontrolled trial 46 depressed patients (39 endogenous depressions, 5 schizoaffective psychoses and 2 paranoic schizophrenics with depressive syndromes) were treated for 43 days on the average with 3 X 100 mg 1-[3-(10,11-dihydro-5H-dibenz[b,f]-azepin-5-yl)-propyl]-4-piperidino-piperidine-4-carboxamide-dihydr... | [Activity profile of carpipramine. Results of an open trial and a double-blind trial versus doxepin]. During an uncontrolled trial 46 depressed patients (39 endogenous depressions, 5 schizoaffective psychoses and 2 paranoic schizophrenics with depressive syndromes) were treated for 43 days on the average with 3 X 100 m... | [
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PMID:14656 | Lack of beta-adrenergic activity of isoflurane in the dog: a comparison of circulatory effects of halothane and isoflurane after propranolol administration. | The studies were undertaken to determine whether isoflurance inhalation is associated with a degree of beta-adrenergic action that is potentially important in clinical situations, and to compare the circulatory tolerance to isoflurane and halothane in dogs following beta blockade. We measured arterial and pulmonary art... | Lack of beta-adrenergic activity of isoflurane in the dog: a comparison of circulatory effects of halothane and isoflurane after propranolol administration. The studies were undertaken to determine whether isoflurance inhalation is associated with a degree of beta-adrenergic action that is potentially important in clin... | [
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PMID:14657 | An evaluation of the AVL 937C blood-gas and pH microanalyser. | The AVL 937C blood-gas and pH microanalyser was evaluated with particular reference to its use in obsterics and in neonatal paediatrics in which its ability to analyse blood smaples as small as 40 micronlitre would be of particular value. Analysing samples of cord blood, maternal venous blood and foetal scalp blood, th... | An evaluation of the AVL 937C blood-gas and pH microanalyser. The AVL 937C blood-gas and pH microanalyser was evaluated with particular reference to its use in obsterics and in neonatal paediatrics in which its ability to analyse blood smaples as small as 40 micronlitre would be of particular value. Analysing samples o... | [
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PMID:14658 | Relative amnesic actions of diazepam, flunitrazepam and lorazepam in man. | 1. Ten postcards were shown to groups of ten to twenty patients over 60, 90 or 270 min after intravenous injection of saline, diazepam (10 and 20 mg), flunitrazepam (1 and 2 mg) and lorazepam (4 mg). 2. Incidence of amnesia was estimated by the patients' ability to recall or recognize these cards and to recall various ... | Relative amnesic actions of diazepam, flunitrazepam and lorazepam in man. 1. Ten postcards were shown to groups of ten to twenty patients over 60, 90 or 270 min after intravenous injection of saline, diazepam (10 and 20 mg), flunitrazepam (1 and 2 mg) and lorazepam (4 mg). 2. Incidence of amnesia was estimated by the p... | [
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PMID:14659 | Plasma concentrations of benzodiazepines. | 1. Twenty anxious patients were treated with medazepam, diazepam, chlordiazepoxide, amylobarbitone and placebo, each given in flexible dosage for 2-4 weeks. 2. At the end of each treatment, a series of clinical, physiological and behavioural variables were measured and plasma samples were taken for estimation of the ap... | Plasma concentrations of benzodiazepines. 1. Twenty anxious patients were treated with medazepam, diazepam, chlordiazepoxide, amylobarbitone and placebo, each given in flexible dosage for 2-4 weeks. 2. At the end of each treatment, a series of clinical, physiological and behavioural variables were measured and plasma s... | [
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PMID:14663 | Conformational transition accompanying the binding of Ca2+ to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesterase. | The Ca2+-dependent protein activator of 3':5'-cyclic adenosine monophosphate phosphodiesterase is shown to undergo a conformational transition upon binding of 2 mol of Ca2+/mol of activator. Circular dichroic studies indicate that Ca2+ induces an increase of 5-8% in alpha-helix content with a concomitant decrease in th... | Conformational transition accompanying the binding of Ca2+ to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesterase. The Ca2+-dependent protein activator of 3':5'-cyclic adenosine monophosphate phosphodiesterase is shown to undergo a conformational transition upon binding of 2 mol of Ca2+/mol ... | [
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PMID:14664 | The electrochemical proton gradient in Escherichia coli membrane vesicles. | Membrane vesicles isolated from Escherichia coli grown under various conditions generate a transmembrane pH gradient (delta pH) of about 2 pH units (interior alkaline) under appropriate conditions when assayed by flow dialysis. Using the distribution of weak acids to measure delta pH and the distribution of the lipophi... | The electrochemical proton gradient in Escherichia coli membrane vesicles. Membrane vesicles isolated from Escherichia coli grown under various conditions generate a transmembrane pH gradient (delta pH) of about 2 pH units (interior alkaline) under appropriate conditions when assayed by flow dialysis. Using the distrib... | [
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PMID:14665 | The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. | In the previous paper [ramos, S., and Kaback, H.R. (1977), Biochemistry 16 (preceding paper in this issue)], it was demonstrated that Escherichia coli membrane vesicles generate a large electrochemical proton gradient (delta-muH+) under appropriate conditions, and some of the properties of delta-muH+ and its component ... | The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. In the previous paper [ramos, S., and Kaback, H.R. (1977), Biochemistry 16 (preceding paper in this issue)], it was demonstrated that Escherichia coli membrane vesicles generate a large electrochemic... | [
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PMID:14666 | Studies of individual carbon sites of azurin from Pseudomonas aeruginosa by natural-abundance carbon-13 nuclear magnetic resonance spectroscopy. | The environments of the aromatic residues (and of the single arginine residue) of azurin from Pseudomonas aeruginosa are investigated by means of natural-abundance 13C Fourier transform NMR spectroscopy. In the case of the diamagnetic Cu(I) azurin, all 17 nonprotonated aromatic carbons (and Czota of Arg-79) yield narro... | Studies of individual carbon sites of azurin from Pseudomonas aeruginosa by natural-abundance carbon-13 nuclear magnetic resonance spectroscopy. The environments of the aromatic residues (and of the single arginine residue) of azurin from Pseudomonas aeruginosa are investigated by means of natural-abundance 13C Fourier... | [
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PMID:14667 | Active proton uptake by chromaffin granules: observation by amine distribution and phosphorus-31 nuclear magnetic resonance techniques. | The hydrogen ion activity within isolated chromaffin granules can be estimated from the distribution of the weak base methylamine and from phosphorus-31 nuclear magnetic resonance spectra of ATP contained in the granules. Following the addition of ATP to the external medium, the internal pH drops by 0.2 to 0.5 unit. Th... | Active proton uptake by chromaffin granules: observation by amine distribution and phosphorus-31 nuclear magnetic resonance techniques. The hydrogen ion activity within isolated chromaffin granules can be estimated from the distribution of the weak base methylamine and from phosphorus-31 nuclear magnetic resonance spec... | [
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PMID:14668 | Determination of the microscopic and macroscopic acid dissociation constants of glycyl-L-histidyl-L-lysine and related histidine peptides. | Proton magnetic resonance studies of the acid-base chemistry of the glycyl ammonium, histidyl imidazolium, and lysyl ammonium groups of glycyl-L-histidyl-L-lysine and of the glycyl ammonium and histidyl imidazolium groups of glycyl-L-histidine and glycyl-L-histidylglycine are described. Chemical-shift data indicate tha... | Determination of the microscopic and macroscopic acid dissociation constants of glycyl-L-histidyl-L-lysine and related histidine peptides. Proton magnetic resonance studies of the acid-base chemistry of the glycyl ammonium, histidyl imidazolium, and lysyl ammonium groups of glycyl-L-histidyl-L-lysine and of the glycyl ... | [
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PMID:14669 | Glutamine active site of formylglycinamide ribonucleotide amidotransferase. 1. Labeling of the enzyme with iodoacetate. | A two-step method for labeling the glutamine active site of formyglycinamide ribonucleotide (FGAR) amidotransferase from chicken liver has been developed in which reaction of all other reactive groups with unlabeled iodoacetate is followed by specific labeling of the glutamine site with radioactive reagent. A study of ... | Glutamine active site of formylglycinamide ribonucleotide amidotransferase. 1. Labeling of the enzyme with iodoacetate. A two-step method for labeling the glutamine active site of formyglycinamide ribonucleotide (FGAR) amidotransferase from chicken liver has been developed in which reaction of all other reactive groups... | [
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PMID:14670 | Solvent effects on thiamin-enzyme model interactions. I. Interactions with tryptophan. | The solvent polarity dependence of the interaction between thiamin and tryptophan was studied by spectrophotometric methods. The ultraviolet (UV) data clearly indicate that the interaction is weakened when the complex is transferred from water to aqueous ethanol or aqueous dioxane. The interaction of thiamin and trypto... | Solvent effects on thiamin-enzyme model interactions. I. Interactions with tryptophan. The solvent polarity dependence of the interaction between thiamin and tryptophan was studied by spectrophotometric methods. The ultraviolet (UV) data clearly indicate that the interaction is weakened when the complex is transferred ... | [
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PMID:14671 | NADPH-cytochrome P-450 reductase from rat liver: purification by affinity chromatography and characterization. | (NADPH)-cytochrome P-450 reductase was purified to apparent homogeneity by a procedure utilizing nicotinamide adenine dinucleotide phosphate (NADP)-Sepharose affinity column chromatography. The purified flavoprotein has a molecular weight of 79 700 and catalyzes cytochrome P-450 dependent drug metabolism, as well as re... | NADPH-cytochrome P-450 reductase from rat liver: purification by affinity chromatography and characterization. (NADPH)-cytochrome P-450 reductase was purified to apparent homogeneity by a procedure utilizing nicotinamide adenine dinucleotide phosphate (NADP)-Sepharose affinity column chromatography. The purified flavop... | [
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PMID:14672 | Isotope effect studies of the role of metal ions in isocitrate dehydrogenase. | Pig heart NADP+-dependent isocitrate dehydrogenase requires a metal ion for activity. Under optimum conditions (pH 7.5, Mg2+ present), the carbon isotope effect is k12/k13 = 0.9989 +/- 0.0004 for the carboxyl carbon undergoing decarboxylation and hydrogen isotope effects are VmaxH/VmaxD = 1.09 +/- 0.04 and (Vmax/Km)H/(... | Isotope effect studies of the role of metal ions in isocitrate dehydrogenase. Pig heart NADP+-dependent isocitrate dehydrogenase requires a metal ion for activity. Under optimum conditions (pH 7.5, Mg2+ present), the carbon isotope effect is k12/k13 = 0.9989 +/- 0.0004 for the carboxyl carbon undergoing decarboxylation... | [
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PMID:14673 | pH-dependent Soret difference spectra of the deoxy and carbonmonoxy forms of human hemoglobin and its derivatives. | The transition from deoxy to oxystructure of hemoglobin A (Hb) is accompanied by the breaking of the salt bridges formed by C-terminal residues in deoxy-Hb. This, in turn, changes the state of the heme. The switch between these different allosteric forms can be followed by changes in the optical absorbance spectra (Per... | pH-dependent Soret difference spectra of the deoxy and carbonmonoxy forms of human hemoglobin and its derivatives. The transition from deoxy to oxystructure of hemoglobin A (Hb) is accompanied by the breaking of the salt bridges formed by C-terminal residues in deoxy-Hb. This, in turn, changes the state of the heme. Th... | [
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PMID:14674 | Acid-catalyzed hydration of reduced nicotinamide adenine dinucleotide and its analogues. | The rate of the primary acid modification reaction of 1,4-dihydronicotinamide adenine dinucleotide (NADH) and 1,4-dihydro-3-acetylpyridine adenine dinucleotide (APADH) and their analogues has been studied over a wide pH range (pH 1-7) with a variety of general acid catalysts. The rate depends on [H+] at moderate pH and... | Acid-catalyzed hydration of reduced nicotinamide adenine dinucleotide and its analogues. The rate of the primary acid modification reaction of 1,4-dihydronicotinamide adenine dinucleotide (NADH) and 1,4-dihydro-3-acetylpyridine adenine dinucleotide (APADH) and their analogues has been studied over a wide pH range (pH 1... | [
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PMID:14675 | Phosphatidic acid distribution on the external surface of mixed vesicles. | A new method has been developed to detect the distribution of phosphatidic acid on the external surface of mixed phospholipid vesicles. Some positive dyes undergo large absorbance changes when the spatial separation between two or more dye molecules is smaller than a critical distance. When these dyes interact with mix... | Phosphatidic acid distribution on the external surface of mixed vesicles. A new method has been developed to detect the distribution of phosphatidic acid on the external surface of mixed phospholipid vesicles. Some positive dyes undergo large absorbance changes when the spatial separation between two or more dye molecu... | [
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PMID:14676 | Duplex formation of a nonionic oligo(deoxythymidylate) analogue (heptadeoxythymidylyl-(3'-5')-deoxythymidine heptaethyl ester (d-(Tp(Et))7T)) with poly(deoxyadenylate). Evaluation of the electrostatic interaction. | The heptaethyl ester of heptadeoxythymidylyl-(3'-5')-deoxythymidine (d-[Tp(Et)]7T or d-T8-Et) has been prepared by chemical methods. The material, consisting of a mixture of diastereoisomers, forms a 1:1 complex with (dA)n in neutral aqueous buffer; this interaction is virtually independent of ionic strength. The octam... | Duplex formation of a nonionic oligo(deoxythymidylate) analogue (heptadeoxythymidylyl-(3'-5')-deoxythymidine heptaethyl ester (d-(Tp(Et))7T)) with poly(deoxyadenylate). Evaluation of the electrostatic interaction. The heptaethyl ester of heptadeoxythymidylyl-(3'-5')-deoxythymidine (d-[Tp(Et)]7T or d-T8-Et) has been pre... | [
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PMID:14677 | Biosynthesis of spin-labeled peptidoglycan: spin-spin interactions. | Membrane preparations from Gaffkya homari catalyzed the in vitro biosynthesis of soluble uncross-linked spin-labeled peptidoglycan, a uniformly labeled polynitroxide, from the spin-labeled nucleotide UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-2,2,5,5-tetramethyl-1-pyrrolin-1-oxyl-3-carbonyl)-DAla-DAla (I) and UDP-GlcNAc. Soluble... | Biosynthesis of spin-labeled peptidoglycan: spin-spin interactions. Membrane preparations from Gaffkya homari catalyzed the in vitro biosynthesis of soluble uncross-linked spin-labeled peptidoglycan, a uniformly labeled polynitroxide, from the spin-labeled nucleotide UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-2,2,5,5-tetramethyl... | [
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PMID:14678 | Affinity labeling of rabbit muscle pyruvate kinase by 5'-p-fluorosulfonylbenzoyladenosine. | Rabbit muscle pyruvate kinase is irreversibly inactivated upon incubation with the adenine nucleotide analogue, 5'-p-fluorosulfonylbenzoyladenosine. A plot of the time dependence of the logarithm of the enzymatic activity at a given time divided by the initial enzymatic activity(logE/Eo) reveals a biphasic rate of inac... | Affinity labeling of rabbit muscle pyruvate kinase by 5'-p-fluorosulfonylbenzoyladenosine. Rabbit muscle pyruvate kinase is irreversibly inactivated upon incubation with the adenine nucleotide analogue, 5'-p-fluorosulfonylbenzoyladenosine. A plot of the time dependence of the logarithm of the enzymatic activity at a gi... | [
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PMID:14679 | Selective chemical modification of Escherichia coli elongation factor G: butanedione modification of an arginine essential for nucleotide binding. | Treatment of Escherichia coli elongation factor G with the arginine reagent, 2,3-butanedione, leads to the inactivation of the enzyme when performed in sodium borate buffers. The inhibition follows pseudo-first-order kinetics until 95% of the activity has been lost and further incubation results in complete inhibiton. ... | Selective chemical modification of Escherichia coli elongation factor G: butanedione modification of an arginine essential for nucleotide binding. Treatment of Escherichia coli elongation factor G with the arginine reagent, 2,3-butanedione, leads to the inactivation of the enzyme when performed in sodium borate buffers... | [
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PMID:14680 | Light-activated calcium release from sonicated bovine retinal rod outer segment disks. | Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insens... | Light-activated calcium release from sonicated bovine retinal rod outer segment disks. Calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. The amount of calcium liberated is proportional t... | [
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PMID:14681 | A proton magnetic resonance study of the conformation of methionine-enkephalin as a function of pH. | It is found that methionine-enkephalin has at least two different conformations in aqueous solution, one at low and one at high pH. From inspection of titration curves and coupling constant values, it seems reasonable to conclude that these conformations are characterized by a folding so as to bring the two ends of the... | A proton magnetic resonance study of the conformation of methionine-enkephalin as a function of pH. It is found that methionine-enkephalin has at least two different conformations in aqueous solution, one at low and one at high pH. From inspection of titration curves and coupling constant values, it seems reasonable to... | [
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PMID:14682 | Proton translocation in chloroplasts and its relationship to electron transport between the photosystems. | Using dark adapted isolated spinach chloroplasts and sequences of brief saturating flashes the correlation of the uptake and release of protons with electron transport from Photosystem II to Photosystem I were studied. The following observations and conclusions are reported: (1) Flash-induced proton uptake shows a weak... | Proton translocation in chloroplasts and its relationship to electron transport between the photosystems. Using dark adapted isolated spinach chloroplasts and sequences of brief saturating flashes the correlation of the uptake and release of protons with electron transport from Photosystem II to Photosystem I were stud... | [
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PMID:14683 | Light-depending rubidium transport in intact Halobacterium halobium cells. | The uptake of rubidium in intact Halobacterium halobium cells was followed, and found to be light-dependent. The exchange process is slow, the steady-state rate of 86Rb+/Rb+ exchange being given by k. = 6.3 - 10(-4) min-1. Starved cells exhibited a faster rate than unstarved cells. The influx of 86Rb+ was almost comple... | Light-depending rubidium transport in intact Halobacterium halobium cells. The uptake of rubidium in intact Halobacterium halobium cells was followed, and found to be light-dependent. The exchange process is slow, the steady-state rate of 86Rb+/Rb+ exchange being given by k. = 6.3 - 10(-4) min-1. Starved cells exhibite... | [
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PMID:14684 | The pH dependence of the oxidation-reduction midpoint potential of cytochromes c2 in vivo. | A recent report by Pettigrew et al. [Biochim, Biophys. Acta 430, (1976), 197-208] has examined the pH dependence of the oxidation-reduction midpoint potential of cytochromes c2 in vitro. In media of low ionic strength, these workers identified several pKs on the oxidized forms of the cytochromes, and in some cases ther... | The pH dependence of the oxidation-reduction midpoint potential of cytochromes c2 in vivo. A recent report by Pettigrew et al. [Biochim, Biophys. Acta 430, (1976), 197-208] has examined the pH dependence of the oxidation-reduction midpoint potential of cytochromes c2 in vitro. In media of low ionic strength, these work... | [
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PMID:14685 | Acetylation of histones in isolated avian erythroid nuclei. | 1. Suspensions of avian erythroid nuclei, of high purity, were prepared. Acetylation of histones was observed when nuclei were incubated in the presence of [1-14C]acetyl CoA, but not in the presence of sodium [3H]acetate. 2. The acetylation reaction was very heat labile and reproduced the in vivo reaction with high fid... | Acetylation of histones in isolated avian erythroid nuclei. 1. Suspensions of avian erythroid nuclei, of high purity, were prepared. Acetylation of histones was observed when nuclei were incubated in the presence of [1-14C]acetyl CoA, but not in the presence of sodium [3H]acetate. 2. The acetylation reaction was very h... | [
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PMID:14686 | Separation of DNA-dependent DNA polymerase activities in Micrococcus radiodurans. | DNA polymerase activities in Micrococcus radiodurans were separated into two fractions after purification more than 2000 fold. They differ in pH optimum and residual activities in the absence of a full deoxyribonucleoside triphosphates complement. NAD partly inhibited one of the activities. Both activities were eluted ... | Separation of DNA-dependent DNA polymerase activities in Micrococcus radiodurans. DNA polymerase activities in Micrococcus radiodurans were separated into two fractions after purification more than 2000 fold. They differ in pH optimum and residual activities in the absence of a full deoxyribonucleoside triphosphates co... | [
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PMID:14687 | Iodination-deiodination. A radiochemical method for detection of structure and changes in structure in RNA. | Bound iodine is released from radioiodinated nucleotides in polymers exposed to sodium bisulfite. The rate of bisulfite-catalyzed deiodination of pyrimidines can be controlled both by change of temperature of pH and is also dependent on the molecular association of the nucleotide. The rate of release of iodine from iod... | Iodination-deiodination. A radiochemical method for detection of structure and changes in structure in RNA. Bound iodine is released from radioiodinated nucleotides in polymers exposed to sodium bisulfite. The rate of bisulfite-catalyzed deiodination of pyrimidines can be controlled both by change of temperature of pH ... | [
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PMID:14688 | The effect of monovalent and divalent cations on the activity of Streptococcus lactis C10 pyruvate kinase. | The pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Streptococcus lactis C10 had an obligatory requirement for both a monovalent cation and divalent cation. NH+4 and K+ activated the enzyme in a sigmoidal manner (nH =1.55) at similar concentrations, whereas Na+ and Li+ could only weakly activat... | The effect of monovalent and divalent cations on the activity of Streptococcus lactis C10 pyruvate kinase. The pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Streptococcus lactis C10 had an obligatory requirement for both a monovalent cation and divalent cation. NH+4 and K+ activated the enzym... | [
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PMID:14689 | Cyclic nucleotide phosphodiesterases of Hyalophora cecropia silkmoth fat body. | Two soluble forms of 3':5'-cyclic-nucleotide phosphodiesterase (o':5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17) were found in the larval fat body of the silkmoth Hyalophora cecropia. These differ in elution profile on Sephadex G-200, solubility in ammonium sulfate, metal ion requirements and kinetic proper... | Cyclic nucleotide phosphodiesterases of Hyalophora cecropia silkmoth fat body. Two soluble forms of 3':5'-cyclic-nucleotide phosphodiesterase (o':5'-cyclic-nucleotide 5'-nucleotidohydrolase, EC 3.1.4.17) were found in the larval fat body of the silkmoth Hyalophora cecropia. These differ in elution profile on Sephadex G... | [
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PMID:14690 | Influence of pH and sodium on the inhibition of guinea-pig heart (Na+ + K+)-ATPase by calcium. | The inhibition of guinea-pig heart (Na+ + K+)-ATPase (ATP phosphohydrolase EC 3.6.1.3) by calcium has been studied at pH 7.4, 6.8 and 6.4. 1. A decrease in pH reduced the threshold inhibitory concentration of calcium and the calcium concentration producing an inhibition of 50% of the enzyme activity. 2. Calcium reduced... | Influence of pH and sodium on the inhibition of guinea-pig heart (Na+ + K+)-ATPase by calcium. The inhibition of guinea-pig heart (Na+ + K+)-ATPase (ATP phosphohydrolase EC 3.6.1.3) by calcium has been studied at pH 7.4, 6.8 and 6.4. 1. A decrease in pH reduced the threshold inhibitory concentration of calcium and the ... | [
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PMID:14691 | Mn2+-sensitive, soluble adenylate cyclase in rat testis. Differentiation from other testicular nucleotide cyclases. | In subcellular fractions prepared from homogenate of adult rat testis adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) activity was found in the particulate, primarily 600 X g for 10 min, fractions, as well as in the cytosol. The properties of the adenylate cyclase in the cytosol differs substantiall... | Mn2+-sensitive, soluble adenylate cyclase in rat testis. Differentiation from other testicular nucleotide cyclases. In subcellular fractions prepared from homogenate of adult rat testis adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) activity was found in the particulate, primarily 600 X g for 10 mi... | [
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PMID:14692 | Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase. | 1. Serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase were purified from Paracoccus denitrificans strain 8944. 2. Serin transacetylase was purified 150-fold. The enzyme has a pH optimum between 7.5 and 8.0, is specific for L-serine and is inhibited by sulphydryl-group reagents. The apparent Km v... | Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase. 1. Serine transacetylase, O-acetylserine sulphydrylase and beta-cystathionase were purified from Paracoccus denitrificans strain 8944. 2. Serin transacet... | [
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PMID:14693 | Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl-and methionyl-tRNA synthetase. | Cysteinyl- and methionyl-tRNA synthetases (EC 6.11.-) were purified 1200- and 1000-fold, respectively, from sonic extracts of Paracoccus denitrificans strain 8944, and kinetics, substrate specificity and regulatory properties were determined using the ATP-PPi exchange reaction. Both enzymes had pH optima of approx. 8 a... | Sulphur metabolism in Paracoccus denitrificans. Purification, properties and regulation of cysteinyl-and methionyl-tRNA synthetase. Cysteinyl- and methionyl-tRNA synthetases (EC 6.11.-) were purified 1200- and 1000-fold, respectively, from sonic extracts of Paracoccus denitrificans strain 8944, and kinetics, substrate ... | [
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PMID:14694 | The rate of reaction between cytochrome C peroxidase and hydrogen peroxide is not diffusion limited. | The apparent biomolecular rate constant for the cytochrome C peroxidase (EC 1.11.1.5)-hydrogen peroxide reaction has been measured as a function of temperature between 5 and 25 degree C at pH 4,5.5, and 7 and as a function of viscosity over a fifteen-fold range. From the independence of the rate constant on the viscosi... | The rate of reaction between cytochrome C peroxidase and hydrogen peroxide is not diffusion limited. The apparent biomolecular rate constant for the cytochrome C peroxidase (EC 1.11.1.5)-hydrogen peroxide reaction has been measured as a function of temperature between 5 and 25 degree C at pH 4,5.5, and 7 and as a funct... | [
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PMID:14695 | L-glycerol-3-phosphate dehydrogenase from the insect Ceratitis capitata. Purfication, physicochemical and enzymic properties. | Soluble L-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) from the mediterranean fruit fly Ceratitis capitata has been purified 130-fold with an overall yield of about 40%. The final preparation had a specific activity of about 200 mumol NADH/min/mg protein. The enzyme pr... | L-glycerol-3-phosphate dehydrogenase from the insect Ceratitis capitata. Purfication, physicochemical and enzymic properties. Soluble L-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) from the mediterranean fruit fly Ceratitis capitata has been purified 130-fold with an o... | [
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PMID:14696 | Presence and properties of thymidylate synthase in trypanosomatids. | High speed centrifugal supernatant fractions of homogenates of a number of trypanosomatids were assayed for thymidylate synthase (5,10-methylene-tetrahydrofolate: dUMP C-methyltransferase, EC 2.1.1.45) activity using the method of Lomax and Greenberg (1967) J. Biol. Chem. 242, 109-113). Similar activities were detected... | Presence and properties of thymidylate synthase in trypanosomatids. High speed centrifugal supernatant fractions of homogenates of a number of trypanosomatids were assayed for thymidylate synthase (5,10-methylene-tetrahydrofolate: dUMP C-methyltransferase, EC 2.1.1.45) activity using the method of Lomax and Greenberg (... | [
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PMID:14697 | A microacalorimetric study of the interaction between trypsin and sodium n-dodecyl sulphate. | 1. The binding of sodium n-dodecyl sulphate to trypsin and reduced trypsin has been measured by equilibrium dialysis at pH 3.5 and 5.5. 2. At pH 3.5 trypsin specifically binds surfactant at low concentration, at higher concentrations co-operative binding occurs. 3. Reduction of trypsin destroys the specific binding sit... | A microacalorimetric study of the interaction between trypsin and sodium n-dodecyl sulphate. 1. The binding of sodium n-dodecyl sulphate to trypsin and reduced trypsin has been measured by equilibrium dialysis at pH 3.5 and 5.5. 2. At pH 3.5 trypsin specifically binds surfactant at low concentration, at higher concentr... | [
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PMID:14699 | Modification of amino acids and bovine pancreatic ribonuclease A by kethoxal. | Kethoxal (3-ethoxy-2-ketobutanal) reacts with the guanidino group of Nalpha-acetylarginine to produce four derivatives, reactive to periodate, stable at pH 7, with 15% reverting to arginine on acid hydrolysis. Other amino acids with blocked alpha-amino groups do not react, except the epsilon-amino of lysine (slowly). T... | Modification of amino acids and bovine pancreatic ribonuclease A by kethoxal. Kethoxal (3-ethoxy-2-ketobutanal) reacts with the guanidino group of Nalpha-acetylarginine to produce four derivatives, reactive to periodate, stable at pH 7, with 15% reverting to arginine on acid hydrolysis. Other amino acids with blocked a... | [
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PMID:14700 | Low pH stability of alpha-1-antititrypsin. | According to the previous findings of others, the trypsin inhibitory capacity of alpha-1-antitrypsin is irreversibly lost in acidic solutions below pH 5.0. In contrast, experiments reported herein show that considerable inhibitory activity can be regenerated as a time-dependent phenomena following titration to basic me... | Low pH stability of alpha-1-antititrypsin. According to the previous findings of others, the trypsin inhibitory capacity of alpha-1-antitrypsin is irreversibly lost in acidic solutions below pH 5.0. In contrast, experiments reported herein show that considerable inhibitory activity can be regenerated as a time-dependen... | [
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PMID:14702 | The subunit characterization of Callinectes sapidus hemocyanin. | Hemocyanin from the blue crab, Callinectes sapidus, sediments at 25.7 S and has a native molecular weight of 940 000 +/- 20 000. Under solution conditions of increased pH (approximately 10) or ionic strength, the native molecule dissociates to a 17 S species. Reversal of this dissociation was unsuccessful. At pH 10 and... | The subunit characterization of Callinectes sapidus hemocyanin. Hemocyanin from the blue crab, Callinectes sapidus, sediments at 25.7 S and has a native molecular weight of 940 000 +/- 20 000. Under solution conditions of increased pH (approximately 10) or ionic strength, the native molecule dissociates to a 17 S speci... | [
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PMID:14703 | Heterogeneity in alpha-lactalbumins. I. Human alpha-lactalbumin. | alpha-Lactalbumin from human milk shows an heterogeneous behaviour when subjected to ion exchange chromatography with DEAE-Sephadex. Two components have been separated, showing identical patterns in the following studies: amino acid compositions, fluorescence and circular dichroism spectra, transition temperature of de... | Heterogeneity in alpha-lactalbumins. I. Human alpha-lactalbumin. alpha-Lactalbumin from human milk shows an heterogeneous behaviour when subjected to ion exchange chromatography with DEAE-Sephadex. Two components have been separated, showing identical patterns in the following studies: amino acid compositions, fluoresc... | [
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PMID:14704 | Relationship between culture density and catabolite repression of an inducible aliphatic amidase in a thermophilic bacillus. | A direct correlation between the absorbance of a thermophilic bacillus and specific amidase activity was observed, which was found to depend on the cell density of the culture rather than on the time of contact of the culture with the inducer. Dilution of high density cultures caused the specific amidase activity to de... | Relationship between culture density and catabolite repression of an inducible aliphatic amidase in a thermophilic bacillus. A direct correlation between the absorbance of a thermophilic bacillus and specific amidase activity was observed, which was found to depend on the cell density of the culture rather than on the ... | [
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PMID:14705 | Biosynthesis of dimethyl selenide from sodium selenite in rat liver and kidney cell-free systems. | A pathway for the synthesis of dimethyl selenide from sodium selenite was studied in rat liver and kidney fractions under anaerobic conditions in the presence of GSH, a NADPH-generating system, and S-adenosylmethionine. Chromatography of liver or kidney soluble fraction on Sephadex G-75 yielded a Fraction C (30,000 mol... | Biosynthesis of dimethyl selenide from sodium selenite in rat liver and kidney cell-free systems. A pathway for the synthesis of dimethyl selenide from sodium selenite was studied in rat liver and kidney fractions under anaerobic conditions in the presence of GSH, a NADPH-generating system, and S-adenosylmethionine. Ch... | [
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PMID:14706 | Cathepsin D of mouse leukemia L1210 cells. Unusual intracellular localization and biochemical properties. | Mouse leukemia L1210 cells contain lysosomes, but cathepsin D, a typical lysosomal enzyme, has an unusual localization. After fractionation of homogenates of L1210 cells by isopycnic density gradient centrifugation, most of the activity for all of the acid hydrolases studied, except cathepsin D, is sedimentable and sho... | Cathepsin D of mouse leukemia L1210 cells. Unusual intracellular localization and biochemical properties. Mouse leukemia L1210 cells contain lysosomes, but cathepsin D, a typical lysosomal enzyme, has an unusual localization. After fractionation of homogenates of L1210 cells by isopycnic density gradient centrifugation... | [
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PMID:14707 | Specific inhibition of the synthesis of putrescine and spermidine by 1,3-diaminopropane in rat liver in vivo. | Chronic administration of 1,3-diaminopropane, a compound inhibiting mammalian ornithine decarboxylase (EC 4.1.1.17) in vivo, effectively prevented the large increases in the concentration of putrescine that normally occur during rat liver regeneration. Furthermore, repeated injections of diaminopropane depressed by mor... | Specific inhibition of the synthesis of putrescine and spermidine by 1,3-diaminopropane in rat liver in vivo. Chronic administration of 1,3-diaminopropane, a compound inhibiting mammalian ornithine decarboxylase (EC 4.1.1.17) in vivo, effectively prevented the large increases in the concentration of putrescine that nor... | [
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PMID:14708 | Electron spin relaxation time of Fe(III) in high spin heme proteins. | The electron spin relaxation time of high spin Fe(III), taus, was determined from the frequency dependence (5-100 MHz) of the longitudinal proton relaxation rates of water in solutions of catalase, metmyoglobin and acid ferricytochrome c. In all three high-spin heme proteins the relaxation rates incrased below 25 MHz, ... | Electron spin relaxation time of Fe(III) in high spin heme proteins. The electron spin relaxation time of high spin Fe(III), taus, was determined from the frequency dependence (5-100 MHz) of the longitudinal proton relaxation rates of water in solutions of catalase, metmyoglobin and acid ferricytochrome c. In all three... | [
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PMID:14709 | The 5,5-dimethyloxazolidine-2[14C],4-dione distribution technique and the measurement of intracellular pH in Acer pseudoplatanus cells. | The intracellular pH of suspension-cultured Acer pseudoplatanus cells, was estimated from the distribution of 5,5-dimethyloxazolidine-2[14C],4-dione (DMO) between the culture medium and the cells. The metabolization of DMO in this biological system introduces an error in the calculated intracellular pH value. Three met... | The 5,5-dimethyloxazolidine-2[14C],4-dione distribution technique and the measurement of intracellular pH in Acer pseudoplatanus cells. The intracellular pH of suspension-cultured Acer pseudoplatanus cells, was estimated from the distribution of 5,5-dimethyloxazolidine-2[14C],4-dione (DMO) between the culture medium an... | [
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PMID:14710 | The effect of 5-hydroxytryptamine and other indole derivatives on the formation of adenosine 3',5'-cyclic monophosphate in pigeon erythrocytes. | 1. The effect of insulin, acetylcholine, histamine, 5-hydroxytryptamine and prostaglandins E1, E2 and F2alpha on basal and adrenalin-stimulated cyclic AMP content in intact pigeon erythrocytes was investigated. 2. None of these compounds influenced basal cyclic AMP contest, and only 5-hydroxytryptamine antagonized the ... | The effect of 5-hydroxytryptamine and other indole derivatives on the formation of adenosine 3',5'-cyclic monophosphate in pigeon erythrocytes. 1. The effect of insulin, acetylcholine, histamine, 5-hydroxytryptamine and prostaglandins E1, E2 and F2alpha on basal and adrenalin-stimulated cyclic AMP content in intact pig... | [
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PMID:14711 | Characteristics of the cyclic nucleotide phosphodiesterases of normal and leukemic lymphocytes. | The specific activity of cyclic AMP phosphodiesterase and cyclic GMP phosphodiesterase of leukemic lymphocytes was 5-10-fold greater than that of purified normal lymphocytes or of homogenates of spleen, thymus or lymph nodes of normal mice. This rise was demonstrable over a wide range of substrate concentrations. Both ... | Characteristics of the cyclic nucleotide phosphodiesterases of normal and leukemic lymphocytes. The specific activity of cyclic AMP phosphodiesterase and cyclic GMP phosphodiesterase of leukemic lymphocytes was 5-10-fold greater than that of purified normal lymphocytes or of homogenates of spleen, thymus or lymph nodes... | [
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PMID:14712 | [Electron transfer through the octane/water interface in the presence of chlorophyll]. | The data obtained by the vibrating electrode method in the investigation of the system octane/water containing chlorophyll adsorbed on the interface and redox systems in both phases show that chlorophyll is capable in the absence of illumination to transfer electrons from one redox reaction to another one, injecting ch... | [Electron transfer through the octane/water interface in the presence of chlorophyll]. The data obtained by the vibrating electrode method in the investigation of the system octane/water containing chlorophyll adsorbed on the interface and redox systems in both phases show that chlorophyll is capable in the absence of ... | [
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PMID:14717 | [Adenylate kinase of plants. Properties of adenylate kinase of pea leaves]. | The properties of adenylate kinase in 2 ADP in equilibrium ATP + AMP reaction have been studied. The dependence of the enzyme activity on medium pH, protein concentration, substrates, Mg++ ions, AMP, adenine and adenosine has been also investigated. pH optimum is found to be 8.5 for forward reaction and 8-9--for the re... | [Adenylate kinase of plants. Properties of adenylate kinase of pea leaves]. The properties of adenylate kinase in 2 ADP in equilibrium ATP + AMP reaction have been studied. The dependence of the enzyme activity on medium pH, protein concentration, substrates, Mg++ ions, AMP, adenine and adenosine has been also investig... | [
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PMID:14713 | [Several physical aspects of intracellular energy transformation]. | Physical principles underlying the chemiosmotic hypothesis of membrane phosphorylation are analysed. The utilization of free energy determined by dydrogen ions concentration difference across the membrane for ATP synthesis requires ATP-synthetase to be an entropy machine able to perform work on account of heat. The che... | [Several physical aspects of intracellular energy transformation]. Physical principles underlying the chemiosmotic hypothesis of membrane phosphorylation are analysed. The utilization of free energy determined by dydrogen ions concentration difference across the membrane for ATP synthesis requires ATP-synthetase to be ... | [
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PMID:14718 | [Effect of pH on the kinetics of the reaction catalyzed by beta-D-hexosaminidase from Halocynthia roretzi]. | The analysis of the pH-dependence of Michaelis constant and maximal velocity for the reaction catalyzed by beta-D-hexosaminidase from Halocynthia roretzi revealed two essential ionazable groups of the enzyme. One of them controls substrate binding and has a pKa of 5.5 in free enzyme. The other group (pKa=5.6) is necess... | [Effect of pH on the kinetics of the reaction catalyzed by beta-D-hexosaminidase from Halocynthia roretzi]. The analysis of the pH-dependence of Michaelis constant and maximal velocity for the reaction catalyzed by beta-D-hexosaminidase from Halocynthia roretzi revealed two essential ionazable groups of the enzyme. One... | [
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PMID:14714 | [Structural lability of biomembranes and their components studied by fluorescent analysis. V. Liposomes]. | The nature of binding of ionic and neutral fluorescent probes in liposome structures of natural lecithin at various pH and ionic strength of the medium. Temperature relationship of fluorescence intensity of both probes presented in Arrhenius coordinates is shown to have bends at 32--42degrees C. A smaller bend in the t... | [Structural lability of biomembranes and their components studied by fluorescent analysis. V. Liposomes]. The nature of binding of ionic and neutral fluorescent probes in liposome structures of natural lecithin at various pH and ionic strength of the medium. Temperature relationship of fluorescence intensity of both pr... | [
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PMID:14719 | [Conditions of spontaneous gelatinization of sarcoplasmic proteins]. | Ca2+ ions at low concentration (10(-4)-10(-5) M) when added to ultracentrifugates of sarcoplasmic proteins with minimal content of sarcoplasmic reticulum fragments completely prevent protein gelatinization or gels, formed in these conditions, quickly dilute. Ca2+ ions at the concentration of 10(-3) M prevented in these... | [Conditions of spontaneous gelatinization of sarcoplasmic proteins]. Ca2+ ions at low concentration (10(-4)-10(-5) M) when added to ultracentrifugates of sarcoplasmic proteins with minimal content of sarcoplasmic reticulum fragments completely prevent protein gelatinization or gels, formed in these conditions, quickly ... | [
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PMID:14716 | [Effect of Ca ions and pH on the osmotic potential across the epithelium of the small intestine]. | It was found that kinetic features of the behaviour of osmotic potential differences through epithelial cells layer were characteristic of determination of mechanic properties of separate structures of intercellular junctions. The quantitative estimation of the contribution of tight junction and simple junctions to the... | [Effect of Ca ions and pH on the osmotic potential across the epithelium of the small intestine]. It was found that kinetic features of the behaviour of osmotic potential differences through epithelial cells layer were characteristic of determination of mechanic properties of separate structures of intercellular juncti... | [
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0.00... |
PMID:14720 | [Isolation and properties of tripolyphosphatase from Neurospora crassa]. | Homogenous preparation of tripolyphosphatase from Neurospora crassa is obtained. The enzyme is found to consist of two equal subunits with molecular weight of 40 000 and to have pH optimum 7.0 and temperature optimum 50 degrees C. Bivalent metal ions are required for its catalytical activity, the hest activators being ... | [Isolation and properties of tripolyphosphatase from Neurospora crassa]. Homogenous preparation of tripolyphosphatase from Neurospora crassa is obtained. The enzyme is found to consist of two equal subunits with molecular weight of 40 000 and to have pH optimum 7.0 and temperature optimum 50 degrees C. Bivalent metal i... | [
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0.0... |
PMID:14721 | [Isolation and some properties of Ca2+-, Mg2+-dependent deoxyribonuclease from sea urchin (Strongylocentrotus intermedius) embryos]. | Ca2+-Mg2+-dependent deoxyribonuclease (deoxyribonucleate-5'-oligonucleotidehydrolase E. C. 3.1.4.5). Molecular weight of the enzyme is found to be 40 000 daltons isoelectric point--4.4. The enzyme degraded DNA only in the presence of bivalent cations. It hydrolyses preferentially native DNA with pH optimum 7.0-7.2 in t... | [Isolation and some properties of Ca2+-, Mg2+-dependent deoxyribonuclease from sea urchin (Strongylocentrotus intermedius) embryos]. Ca2+-Mg2+-dependent deoxyribonuclease (deoxyribonucleate-5'-oligonucleotidehydrolase E. C. 3.1.4.5). Molecular weight of the enzyme is found to be 40 000 daltons isoelectric point--4.4. T... | [
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PMID:14722 | [Protease from bovine spleen with kininogenase activity]. | A protease with kininogenase activity at pH 7.5 was isolated from bovine spleen extract by gel filtration and ion exchange chromatography. The protease was found in the fraction with molecular weight lower than 25.000 and was separated from the other neutral SH-dependent protease by chromatography on KM-cellulose. The ... | [Protease from bovine spleen with kininogenase activity]. A protease with kininogenase activity at pH 7.5 was isolated from bovine spleen extract by gel filtration and ion exchange chromatography. The protease was found in the fraction with molecular weight lower than 25.000 and was separated from the other neutral SH-... | [
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PMID:14723 | [The activity of beta-hydroxy-beta-methylglutaryl-CoA reductase in the soluble fraction of rat liver]. | Assay conditions are worked out for determination of activity of beta-hydroxy-beta-methylglutaryl-CoA reductase (HMG-CoA reductase) in 140.000 g supernatant fraction of the rat liver. Some kinetic properties of the enzyme are studied: the activity dependency on the incubation time, protein concentration, pH, glutathion... | [The activity of beta-hydroxy-beta-methylglutaryl-CoA reductase in the soluble fraction of rat liver]. Assay conditions are worked out for determination of activity of beta-hydroxy-beta-methylglutaryl-CoA reductase (HMG-CoA reductase) in 140.000 g supernatant fraction of the rat liver. Some kinetic properties of the en... | [
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PMID:14724 | [cAMP phosphodiesterase from phototrophic bacteria Rhodospirillum rubrum]. | cAMP phosphodiesterase activity is discovered in supernatant of R. rubrum cell homogenate after centrifugation at 1000 g. The enzyme is highly active (5.62 nmoles/mg of protein per 1 min) at a broad pH range--from 7.0 to 9.0. The enzyme activity is strongly inhibited with caffeine and dithiotreitol and very significant... | [cAMP phosphodiesterase from phototrophic bacteria Rhodospirillum rubrum]. cAMP phosphodiesterase activity is discovered in supernatant of R. rubrum cell homogenate after centrifugation at 1000 g. The enzyme is highly active (5.62 nmoles/mg of protein per 1 min) at a broad pH range--from 7.0 to 9.0. The enzyme activity... | [
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PMID:14725 | [Influence of dinitrophenol, octanol and toluene upon pH-dependence of ca-ATPase activity of heavy meromyosin]. | It is found that dinitrophenol, octanol and toluene produce similar effects on pH-dependence of ATPase of myosin and heavy meromyosin (HMM), i.e. they decrease or remove the neutral suppression of ATPase activity. The appearance of pH-dependence curves is simplified and approaches the form, which is characteristic for ... | [Influence of dinitrophenol, octanol and toluene upon pH-dependence of ca-ATPase activity of heavy meromyosin]. It is found that dinitrophenol, octanol and toluene produce similar effects on pH-dependence of ATPase of myosin and heavy meromyosin (HMM), i.e. they decrease or remove the neutral suppression of ATPase acti... | [
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PMID:14726 | [Mechanism of amylase action on glucoside starch bonds]. | Functional groups of glucoamylase and alpha-amylase from Asp. awamori, alpha-amylase from Asp. oryzae and alpha- and beta-amylases from barley malt are identified. Kinetic curves of the activity dependency on pH, values of ionization heats and photooxidative inactivation draw to the conclusion that carboxyl-imidazole s... | [Mechanism of amylase action on glucoside starch bonds]. Functional groups of glucoamylase and alpha-amylase from Asp. awamori, alpha-amylase from Asp. oryzae and alpha- and beta-amylases from barley malt are identified. Kinetic curves of the activity dependency on pH, values of ionization heats and photooxidative inac... | [
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PMID:14727 | [Serine proteases from Bac. subtilis]. | Using biospecific adsorbent and subsequent gel-filtration of Sephadex G-75 three fractions of serine proteases (I--III) having different physicochemical properties were isolated from Bac. subtilis. The first protease had molecular weight of 23000--24000 (pH optimum 6,5, activation energy 16,6 ccal/mol. The second one h... | [Serine proteases from Bac. subtilis]. Using biospecific adsorbent and subsequent gel-filtration of Sephadex G-75 three fractions of serine proteases (I--III) having different physicochemical properties were isolated from Bac. subtilis. The first protease had molecular weight of 23000--24000 (pH optimum 6,5, activation... | [
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PMID:14728 | [Methylation of liver cell chromatin proteins at different stages of post-natal development of albino rats]. | Methylation of acid proteins and various histone fractions of liver cell chromatin was studied. The intensity of methylation of acid proteins of animals, differing in age, was shown to be 8--16 times higher than that for histone methylation, despite the fact that the rates of 2-14C-methionine incorporation into these t... | [Methylation of liver cell chromatin proteins at different stages of post-natal development of albino rats]. Methylation of acid proteins and various histone fractions of liver cell chromatin was studied. The intensity of methylation of acid proteins of animals, differing in age, was shown to be 8--16 times higher than... | [
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PMID:14729 | [Cytosolic alcohol dehydrogenases from yeast Torulopsis candida]. | A comparative study of cell cytosol alcohol dehydrogenase (ADH) from yeast Torulopsis candida IBFM-Y-127 grown on glucose and hexadecane which were the only source of carbon, was made. In both cases ADH had a pH optimum within the range of 7.0--10.0, when various normal primary alcohols (C2--C16) were used. The enzyme ... | [Cytosolic alcohol dehydrogenases from yeast Torulopsis candida]. A comparative study of cell cytosol alcohol dehydrogenase (ADH) from yeast Torulopsis candida IBFM-Y-127 grown on glucose and hexadecane which were the only source of carbon, was made. In both cases ADH had a pH optimum within the range of 7.0--10.0, whe... | [
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PMID:14730 | [Human serum albumin: microheterogeneity and hemin-binding ability]. | Microheterogeneity of human serum albumin was studied by isoelectric focussing in ampholines and the borax-borate buffer-mannite system within the pH range 4.0--6.0 and by fractional precipitation in 3 M KCl. Albumin was found to have the same degree of heterogeneity in all separating systems. However, during focussing... | [Human serum albumin: microheterogeneity and hemin-binding ability]. Microheterogeneity of human serum albumin was studied by isoelectric focussing in ampholines and the borax-borate buffer-mannite system within the pH range 4.0--6.0 and by fractional precipitation in 3 M KCl. Albumin was found to have the same degree ... | [
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PMID:14731 | [Nuclear ribonucleases and post-transcriptional changes of RNA. Specificity and other properties of rat liver nuclear endonuclease]. | Some physico-chemical properties, specificity and the character of action of rat liver nuclear ribonuclease are studied. The enzyme maximal activity was observed at pH 7.5--8.0, ionic strength 0.02--0.3, Mg2+ being necessary. Nuclease is an oligomer, having molecular weight is 160000--180000 daltons and containing sepa... | [Nuclear ribonucleases and post-transcriptional changes of RNA. Specificity and other properties of rat liver nuclear endonuclease]. Some physico-chemical properties, specificity and the character of action of rat liver nuclear ribonuclease are studied. The enzyme maximal activity was observed at pH 7.5--8.0, ionic str... | [
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PMID:14732 | [Pterin-protein complex and ferredoxin as possible components of a molecular complex in photosystem I]. | Properties are studied of pterin-protein complex (PPC), possible precursor of ferredoxin in electrone transport chain PS-I. PPC is shown to form chelate complex with Fe ions which are capable to activate the process of light reduction of NADP+ by chloroplasts in the absence of ferrodoxin. A possibility of dark reductio... | [Pterin-protein complex and ferredoxin as possible components of a molecular complex in photosystem I]. Properties are studied of pterin-protein complex (PPC), possible precursor of ferredoxin in electrone transport chain PS-I. PPC is shown to form chelate complex with Fe ions which are capable to activate the process ... | [
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PMID:14733 | [Isolation and properties of cytoplasmic L(+)-lactatoxydase of Candida lipolytica yeasts]. | The enzyme preparation of L(+)-lactatoxydase (K.F. 1.1.3.2) with molecular weight of 230 000 has been isolated from the soluble fraction of the C. lipolytica cells and purified similar 360 times. The enzyme oxydizes L(+)-lactate, the optimum activity of the enzyme being observed at pH 8.0. Oxydation of the substrate is... | [Isolation and properties of cytoplasmic L(+)-lactatoxydase of Candida lipolytica yeasts]. The enzyme preparation of L(+)-lactatoxydase (K.F. 1.1.3.2) with molecular weight of 230 000 has been isolated from the soluble fraction of the C. lipolytica cells and purified similar 360 times. The enzyme oxydizes L(+)-lactate,... | [
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PMID:14734 | [The pH dependence of kinetic parameters of Penicillium brevicompactum RNAase]. | The effect of pH on the kinetic parameters (Km and Ki) for extracellular acid Penicillium brevicompactum RNAse (pH max 4.7+/-0.1), non-specific to the chemical nature of nucleic bases, was studied. The pKm--pH dependence curve showed bends within the following intervals of pH: 3.5--4.0 and 5.6--6.0 (upward side concavi... | [The pH dependence of kinetic parameters of Penicillium brevicompactum RNAase]. The effect of pH on the kinetic parameters (Km and Ki) for extracellular acid Penicillium brevicompactum RNAse (pH max 4.7+/-0.1), non-specific to the chemical nature of nucleic bases, was studied. The pKm--pH dependence curve showed bends ... | [
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PMID:14735 | [Alkaline activation of myosin ATPase: some thermodynamic characteristics]. | The increase in temperature leads to a decrease in pKa of the group responsible for the activation of CaATP2- hydrolysis by myosin in the alkaline zone of pH. At 20-25 degrees the pKa value is about 9. The value of ionization heat (deltaHi) calculated from pKa temperature dependence is 7.6+/-+/-0.8 kcal/mol. These valu... | [Alkaline activation of myosin ATPase: some thermodynamic characteristics]. The increase in temperature leads to a decrease in pKa of the group responsible for the activation of CaATP2- hydrolysis by myosin in the alkaline zone of pH. At 20-25 degrees the pKa value is about 9. The value of ionization heat (deltaHi) cal... | [
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PMID:14736 | [Sulfhydryl groups of L-asparaginase A from Pseudomonas fluorescens AG]. | It has been demonstrated that the activity of asparaginase A from Ps. fluorescens AG is completely inhibited by 10(-4) M p-chloromercurybenzoate and by 70-85% by Zn2+, Ca2+ and Cu2+ (2.10(-2) M). Iodoacetate, iodoacetamide, N-ethylimide of maleic acid and 5,5'-dithiobis-(2-nitrobenzoic acid) do not decrease the enzyme ... | [Sulfhydryl groups of L-asparaginase A from Pseudomonas fluorescens AG]. It has been demonstrated that the activity of asparaginase A from Ps. fluorescens AG is completely inhibited by 10(-4) M p-chloromercurybenzoate and by 70-85% by Zn2+, Ca2+ and Cu2+ (2.10(-2) M). Iodoacetate, iodoacetamide, N-ethylimide of maleic ... | [
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PMID:14738 | Effect of hypoxia on monoamine synthesis in brains of developing rats. | Tyrosine and tryptophan hydroxylase activity was studied in the postnatal rat brain in vivo by measuring the accumulation of dihydroxyphenylalanine and 5-hydroxytryptophan, respectively after inhibition of L-aromatic amino acid decarboxylase with NSD 1015. With increasing age there was a significant increase in the amo... | Effect of hypoxia on monoamine synthesis in brains of developing rats. Tyrosine and tryptophan hydroxylase activity was studied in the postnatal rat brain in vivo by measuring the accumulation of dihydroxyphenylalanine and 5-hydroxytryptophan, respectively after inhibition of L-aromatic amino acid decarboxylase with NS... | [
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PMID:14740 | Indication of the metarhodopsin I-II transition by absorption-changes of Eriochromblack T. | The dye eriochromblack T (erio T), added to an aqueous suspension of bovine retinal outer segments solubilized by digitonin, shows a light-induced absorption-increase at lambda = 645 nm. Erio T is shown to directly interact with miscellar metarhodopsin I and metarhodopsin II. The absorption-changes of erio T can be reg... | Indication of the metarhodopsin I-II transition by absorption-changes of Eriochromblack T. The dye eriochromblack T (erio T), added to an aqueous suspension of bovine retinal outer segments solubilized by digitonin, shows a light-induced absorption-increase at lambda = 645 nm. Erio T is shown to directly interact with ... | [
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PMID:14743 | Nonporous magnetic materials as enzyme supports: studies with immobilized chymotrypsin. | Chymotrypsin has been immobilized to several nonporous magnetic materials. Nickel particles were considered to be most suitable as immobilized enzyme supports. Chymotrypsin immobilized to nonporous magnetic supports was not fouled significantly by either whole milk or clarified yeast homogenate. AE-cellulose-chymotryps... | Nonporous magnetic materials as enzyme supports: studies with immobilized chymotrypsin. Chymotrypsin has been immobilized to several nonporous magnetic materials. Nickel particles were considered to be most suitable as immobilized enzyme supports. Chymotrypsin immobilized to nonporous magnetic supports was not fouled s... | [
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PMID:14744 | Chemically activated collagen for amyloglucosidase attachment. Use in a helicoidal reactor. | Amyloglucosidase was covalently bound to collagen sheets by a previously described method. The time of acidic methylation (first step of the collagen activation process) was important to obtain a good enzymatic surfacic activity. Homogeneity of the coupling procedure on the surface of collagen films was shown. Some pro... | Chemically activated collagen for amyloglucosidase attachment. Use in a helicoidal reactor. Amyloglucosidase was covalently bound to collagen sheets by a previously described method. The time of acidic methylation (first step of the collagen activation process) was important to obtain a good enzymatic surfacic activity... | [
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PMID:14745 | A fermentation process for producing both ethanol and lysine-enriched yeast. | In 18 batch-fermentation experiments, baker's yeast was grown in an enriched mineral medium, containing 10% by weight glucose, at various pH and temperature levels. The pH and temperature are just two representative engineering variables which can be easily varied at negligible cost. The commercial yeast inoculum, 20% ... | A fermentation process for producing both ethanol and lysine-enriched yeast. In 18 batch-fermentation experiments, baker's yeast was grown in an enriched mineral medium, containing 10% by weight glucose, at various pH and temperature levels. The pH and temperature are just two representative engineering variables which... | [
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0.026... |
PMID:14746 | Production of Schardinger beta-dextrin by soluble and immobilized cyclodextrin glycosyltransferase of an alkalophilic Bacillus sp. | Succinylated cyclodextrin glycosyltransferase (EC 3.2.1.19) of an alkalophilic Bacillus sp. was adsorbed on a vinylpyridine copolymer. The enzyme had about 25% of the activity of soluble enzyme added. No increase of pH or thermal stability of the enzyme was observed by the adsorption, whereas optimum temperature for th... | Production of Schardinger beta-dextrin by soluble and immobilized cyclodextrin glycosyltransferase of an alkalophilic Bacillus sp. Succinylated cyclodextrin glycosyltransferase (EC 3.2.1.19) of an alkalophilic Bacillus sp. was adsorbed on a vinylpyridine copolymer. The enzyme had about 25% of the activity of soluble en... | [
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PMID:14747 | Bacteriolysis by immobilized enzymes. | Bacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. Relative activity of the bacteriolytic enzyme... | Bacteriolysis by immobilized enzymes. Bacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus, and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme-collagen membrane. Relativ... | [
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PMID:14748 | Aminoacylase pellets. | Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The aminoacylase pellets were stable in pH 4-8 but they were unstable in alkaline conditions. The aminoacy... | Aminoacylase pellets. Aminoacylase was immobilized on the mycelium pellets of Aspergillus ochraceus by using albumin and glutaraldehyde. No difference in the optimum pH was observed between native aminoacylase and aminoacylase pellets. The aminoacylase pellets were stable in pH 4-8 but they were unstable in alkaline co... | [
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PMID:14749 | Properties of enzymes immobilized by the diazotized m-diaminobenzene method. | Some properties of a number of enzymes immobilized by the diazotized m-diaminobenzene (dDAB) method are described. The pH-activity profiles of beta-D-glucosidase, glucoamylase, peroxidase, uricase, and D-glucose oxidase were virtually unchanged on immobilization while those of catalase and dextranase were significantly... | Properties of enzymes immobilized by the diazotized m-diaminobenzene method. Some properties of a number of enzymes immobilized by the diazotized m-diaminobenzene (dDAB) method are described. The pH-activity profiles of beta-D-glucosidase, glucoamylase, peroxidase, uricase, and D-glucose oxidase were virtually unchange... | [
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PMID:14750 | Characteristics of yeast invertase immobilized on porous cellulose beads. | Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature profiles were fairly similar up to 55 degrees C. However, above this tempera... | Characteristics of yeast invertase immobilized on porous cellulose beads. Invertase from Candida utilis was immobilized on porous cellulose beads by an ionic-quanidino bond. The immobilized invertase showed optimum activity between pH 4.0 and 5.4, while the free enzyme had a sharp optimum at pH 4.1. Both temperature pr... | [
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PMID:14751 | One hundred patients with acute leukemia treated by chemotherapy, total body irradiation, and allogeneic marrow transplantation. | One hundred patients, 54 with acute myelogenous leukemia (AML) and 46 with acute lymphoblastic leukemia (ALL), considered to be in the end stages of their disease, after combination chemotherapy were treated by marrow transplantation. All patients were given a marrow graft from an HLA-identical sibling after receiving ... | One hundred patients with acute leukemia treated by chemotherapy, total body irradiation, and allogeneic marrow transplantation. One hundred patients, 54 with acute myelogenous leukemia (AML) and 46 with acute lymphoblastic leukemia (ALL), considered to be in the end stages of their disease, after combination chemother... | [
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PMID:14756 | [Mucociliary function and its alteration by certain irritants (author's transl)]. | Mucociliary clearance is usually estimated in vivo by the measurement of mucus transfer or by clearance of radio-active tracers previously deposited in the airways. These methods are relatively difficult and complex so that for routine studies measures are usually made of ciliary activity. To clarify the consequences o... | [Mucociliary function and its alteration by certain irritants (author's transl)]. Mucociliary clearance is usually estimated in vivo by the measurement of mucus transfer or by clearance of radio-active tracers previously deposited in the airways. These methods are relatively difficult and complex so that for routine st... | [
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PMID:14757 | [La culture cellulaire en phase gazeuse. Un nouveau modèle expérimental d'étude in vitro des activités des macrophages alvéolaires]. | Alveolar macrophages, collected by bronchopulmonary lavage and deposited on a membrane filter applied to the surface of a reservoir filled with nutrient medium, were maintained alive in direct contact with the atmosphere. Studies of morphology (using both optic and electron microscopy), of bactericidal activity and of ... | [La culture cellulaire en phase gazeuse. Un nouveau modèle expérimental d'étude in vitro des activités des macrophages alvéolaires]. Alveolar macrophages, collected by bronchopulmonary lavage and deposited on a membrane filter applied to the surface of a reservoir filled with nutrient medium, were maintained alive in d... | [
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PMID:14761 | A simple and effective treatment for flight phobia. | Seventeen patients were treated by group desensitization and autohypnotic training over a period of six weeks for flight phobia, with a highly significant reduction of fear for the group. Expectation of benefit correlated positively with fear reduction. | A simple and effective treatment for flight phobia. Seventeen patients were treated by group desensitization and autohypnotic training over a period of six weeks for flight phobia, with a highly significant reduction of fear for the group. Expectation of benefit correlated positively with fear reduction. | [
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PMID:14762 | Peptides with morphine-like action in the brain. | The reasons which led to the search in the brain for substances with morphine-like actions actions are discussed. Two pentapeptides, methionineenkephalin and leucine-enkephalin, were isolated. The amino acid sequence of methionine-enkephalin occurs also in the pituitary prohormone beta-lipotropin, of which longer fragm... | Peptides with morphine-like action in the brain. The reasons which led to the search in the brain for substances with morphine-like actions actions are discussed. Two pentapeptides, methionineenkephalin and leucine-enkephalin, were isolated. The amino acid sequence of methionine-enkephalin occurs also in the pituitary ... | [
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PMID:14764 | Drain-cover injuries in children. | This report highlights unnecessary injuries to the hands, forearms, and feet of small children, caused by falling drain-cover gratings, colloquially known as "shores". It is suggested that a simple locking device, similar to that employed on manhole covers, would eliminate this type of injury. | Drain-cover injuries in children. This report highlights unnecessary injuries to the hands, forearms, and feet of small children, caused by falling drain-cover gratings, colloquially known as "shores". It is suggested that a simple locking device, similar to that employed on manhole covers, would eliminate this type of... | [
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0.0014358502812683582,
0.03317... |
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