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PMID:15496 | [Natural antiallergenic factors in bile]. | Two groups of substances having an antihistaminic activity were found on purifying bovine bile. It has already been found that bile acids inhibit contraction of guinea pig ileum; another principle has been isolated which is one thousand times more active than the bile acids; this (these) substance(s)--SAN (" substance ... | [Natural antiallergenic factors in bile]. Two groups of substances having an antihistaminic activity were found on purifying bovine bile. It has already been found that bile acids inhibit contraction of guinea pig ileum; another principle has been isolated which is one thousand times more active than the bile acids; th... | [
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PMID:15495 | [Role of adherent cells in GVH induced immunosuppression]. | Two different mechanisms seem to be involved. In early GVH there is apparently an amplification of a normal inhibitory function of a cells. In more advanced GVH immunosuppression seem to be caused by a GVH induced suppressive activity of A cells. Both activities seem to be mediated by soluble factors. | [Role of adherent cells in GVH induced immunosuppression]. Two different mechanisms seem to be involved. In early GVH there is apparently an amplification of a normal inhibitory function of a cells. In more advanced GVH immunosuppression seem to be caused by a GVH induced suppressive activity of A cells. Both activitie... | [
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PMID:15497 | [Spleen reactivity after BCG treatement: test and strain dependency of the response]. | According to the strain used, spleen cells from mice injected two weeks before with 3 mg BCG, when they are engaged in a graft-versus-host reaction show a decrease in reactivity (C57Bl/6or AKR), an increase (DBA/2, BALB/C or SJL/J) or no modification (CBA, C3H). The in vitro responsiveness to PHA and conA of the treate... | [Spleen reactivity after BCG treatement: test and strain dependency of the response]. According to the strain used, spleen cells from mice injected two weeks before with 3 mg BCG, when they are engaged in a graft-versus-host reaction show a decrease in reactivity (C57Bl/6or AKR), an increase (DBA/2, BALB/C or SJL/J) or... | [
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PMID:15502 | [Macrokinetic equations of pH effect on the growth of Actinomyces aureofaciens and biosynthesis of tetracycline]. | The effect of pH on the culture respiration rate at different concentrations of glucose in the medium was studied. It was found that the hydrogen ions showed their effect irrespective of the subsrate concentration in the medium. In this connection a type of macrokinetic equations of the effect of pH on the growth and a... | [Macrokinetic equations of pH effect on the growth of Actinomyces aureofaciens and biosynthesis of tetracycline]. The effect of pH on the culture respiration rate at different concentrations of glucose in the medium was studied. It was found that the hydrogen ions showed their effect irrespective of the subsrate concen... | [
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PMID:15503 | [Study of penicillin amidase from E. coli. pH-dependence of kinetic parameters of enzymatic hydrolysis of benzylpenicillin]. | The authors studies pH-dependencies of the kinetic parameters (Vm, KM, Vm/KM) and constants of competitive inhibition by phenylacetic acid of penicillinamidase-catalyzed hydrolysis of benzylpenicillin. The experimental data are in agreement with the assumption according to which there are 3 equilibrium ionogenic forms ... | [Study of penicillin amidase from E. coli. pH-dependence of kinetic parameters of enzymatic hydrolysis of benzylpenicillin]. The authors studies pH-dependencies of the kinetic parameters (Vm, KM, Vm/KM) and constants of competitive inhibition by phenylacetic acid of penicillinamidase-catalyzed hydrolysis of benzylpenic... | [
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PMID:15504 | [Chromatographic separation of rifamycins on a thin sorbent thin layer]. | A method of simultaneous separation of rifampicins O, S and rifamicin derivates S, SV in a thin layer of silica gel KSK No. 2 impregnated with acetate buffer, pH 4.6 in a solvent system chlorophorm-methanol (100:5) was developed. The minimum detectable amount of the compounds studied was 1--2gamma, when the coloured zo... | [Chromatographic separation of rifamycins on a thin sorbent thin layer]. A method of simultaneous separation of rifampicins O, S and rifamicin derivates S, SV in a thin layer of silica gel KSK No. 2 impregnated with acetate buffer, pH 4.6 in a solvent system chlorophorm-methanol (100:5) was developed. The minimum detec... | [
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PMID:15505 | [Penicillin amidase from E. coli. A direct spectrophotometric method of determining the enzyme's activity]. | A method for determination of the enzymatic activity of penicillinamidas (PA) based on spectrophotometric estimation of the stained product amount produced in hydrolysis of 4-phenylacetamido-2-nitrobenzoic acid (PANBA) catalyzed by the enzyme is proposed. Some physico-chemical properties of the substrate and the staine... | [Penicillin amidase from E. coli. A direct spectrophotometric method of determining the enzyme's activity]. A method for determination of the enzymatic activity of penicillinamidas (PA) based on spectrophotometric estimation of the stained product amount produced in hydrolysis of 4-phenylacetamido-2-nitrobenzoic acid (... | [
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PMID:15506 | [Simplified liquid nutrient media for controlling antibiotic activity, the spectrum of their antibacterial action and the sensitivity of microorganisms]. | Beaf-peptone broth and some of its modifications, one of which is a simple and in expensive one to a leser extent binding to antibiotics, such as penicillin, oxytetracycline and streptomycin and providing sufficient growth of the test microbes were used to determine the antibiotic activity with the methods of serial di... | [Simplified liquid nutrient media for controlling antibiotic activity, the spectrum of their antibacterial action and the sensitivity of microorganisms]. Beaf-peptone broth and some of its modifications, one of which is a simple and in expensive one to a leser extent binding to antibiotics, such as penicillin, oxytetra... | [
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PMID:15507 | [Experimental study of a gentamicin aerosol]. | The study of gentamicin aerosol showed its relative innocuousness: it did not inhibit the growth and development of young animals, did not induce pathological changes in the upper respiratory tract, kidneys, liver, heart and spleen on its prolonged use. Pathohistological examination revealed slight irritating effect of... | [Experimental study of a gentamicin aerosol]. The study of gentamicin aerosol showed its relative innocuousness: it did not inhibit the growth and development of young animals, did not induce pathological changes in the upper respiratory tract, kidneys, liver, heart and spleen on its prolonged use. Pathohistological ex... | [
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PMID:15509 | Effect of culture conditions on synthesis of L-asparaginase by Escherichia coli A-1. | The nutritional requirements and culture conditions affecting biosynthesis of L-asparaginase in a mutant of Escherichia coli HAP designated strain A-1 were studied. Asparaginase activity was increased by the addition of L-glutamic acid, L-glutamine, or commercial-grade monosodium glutamate. The rate of enzyme synthesi... | Effect of culture conditions on synthesis of L-asparaginase by Escherichia coli A-1. The nutritional requirements and culture conditions affecting biosynthesis of L-asparaginase in a mutant of Escherichia coli HAP designated strain A-1 were studied. Asparaginase activity was increased by the addition of L-glutamic aci... | [
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PMID:15510 | Growth characteristics of a new methylomonad. | A methylomonad culture was isolated from pond water and examined as a potential source of single-cell protein. A medium containing magnesium sulfate, ammonium hydroxide, sodium phosphate, tap water, and methanol supported the growth of the isolate. Optimal growth conditions in batch cultures for the organism were: temp... | Growth characteristics of a new methylomonad. A methylomonad culture was isolated from pond water and examined as a potential source of single-cell protein. A medium containing magnesium sulfate, ammonium hydroxide, sodium phosphate, tap water, and methanol supported the growth of the isolate. Optimal growth conditions... | [
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PMID:15511 | Association of hydrogen metabolism with methanogenesis in Lake Mendota sediments. | Lake Mendota sediments were studied to determine the role of H2 in sediment methanogenesis. H2 was generally not detectable in sediment. The addition of H2 to sediment significantly increased methanogenensis. The amount of methane produced was proportional to the concentration of hydrogen added. H2 addition stimulated ... | Association of hydrogen metabolism with methanogenesis in Lake Mendota sediments. Lake Mendota sediments were studied to determine the role of H2 in sediment methanogenesis. H2 was generally not detectable in sediment. The addition of H2 to sediment significantly increased methanogenensis. The amount of methane produce... | [
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PMID:15512 | Media for identification of Gibberella zeae and production of F-2-(Zearalenone). | Media are described for the isolaton of Fusarium graminearum in the perithecial state, Gibberella zeae, and for the production of F-2 (zearalenone) by Fusarium species. On soil extract-corn meal agar isolated medium, G. Zeae produced perithecia in 9 to 14 days under a 12-h photoperiod. Species of Fusarium were screened... | Media for identification of Gibberella zeae and production of F-2-(Zearalenone). Media are described for the isolaton of Fusarium graminearum in the perithecial state, Gibberella zeae, and for the production of F-2 (zearalenone) by Fusarium species. On soil extract-corn meal agar isolated medium, G. Zeae produced perit... | [
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PMID:15524 | Wilm's tumour, hypospadias, and cryptorchidism in twins. | Twin boys, both of whom had hypospadias and bilateral cryptorchidism, each developed a left-sided Wilms's tumour. The first twin was found to have an advanced multifocal tumour at the age of 15 months and died with local recurrence and pulmonary metastases. The diagnosis was made in the second twin one month later and ... | Wilm's tumour, hypospadias, and cryptorchidism in twins. Twin boys, both of whom had hypospadias and bilateral cryptorchidism, each developed a left-sided Wilms's tumour. The first twin was found to have an advanced multifocal tumour at the age of 15 months and died with local recurrence and pulmonary metastases. The d... | [
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PMID:15525 | Effects of beta-adrenoceptor blocking agents, pindolol, alprenolol and practolol on blood pressure and heart rate in conscious renal hypertensive dogs. | The effects of pindolol, 10 mg/kg, alprenolol, 50 mg/kg, and practolol, 50 mg/kg, given by mouth, on blood pressure and heart rate were investigated over a 24-hr period in 5 conscious renal hypertensive dogs, using a cross-over design. Pindolol and alprenolol caused significant falls in blood pressure which averaged 22... | Effects of beta-adrenoceptor blocking agents, pindolol, alprenolol and practolol on blood pressure and heart rate in conscious renal hypertensive dogs. The effects of pindolol, 10 mg/kg, alprenolol, 50 mg/kg, and practolol, 50 mg/kg, given by mouth, on blood pressure and heart rate were investigated over a 24-hr period... | [
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PMID:15526 | Selective activation of cardiovascular adenylate cyclase in vivo. | Cyclic AMP levels were measured in cardiac and vascular tissues removed from pentobarbitone-anesthetized rats pretreated with theophylline 45 mg/kg i.p. Noradrenaline, 20 microng/kg i.v., increased cardiac but not vascular cyclic AMP levels; this selective effect of noradrenaline was not altered by pretreatment with ph... | Selective activation of cardiovascular adenylate cyclase in vivo. Cyclic AMP levels were measured in cardiac and vascular tissues removed from pentobarbitone-anesthetized rats pretreated with theophylline 45 mg/kg i.p. Noradrenaline, 20 microng/kg i.v., increased cardiac but not vascular cyclic AMP levels; this selecti... | [
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PMID:15527 | Comparative subcutaneous absorption of local anesthetics: lidocaine, procaine and tetracaine. | The pH partition hypothesis was applied to the absorption of ionizable local anesthetic amines through the subcutaneous (s.c.) route. Solutions of lidocaine hydrochloride (LHCl), procaine hydrochloride (PHCl) and tetracaine hydrochloride (THCl) at various pH were implanted on s.c. animal tissue through a glass absorpti... | Comparative subcutaneous absorption of local anesthetics: lidocaine, procaine and tetracaine. The pH partition hypothesis was applied to the absorption of ionizable local anesthetic amines through the subcutaneous (s.c.) route. Solutions of lidocaine hydrochloride (LHCl), procaine hydrochloride (PHCl) and tetracaine hy... | [
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PMID:15528 | Acidostability of speroplasts prepared from Thiobacillus thiooxidans. | Thiobacillus thiooxidans was acidostable even in the absence of its respiratory substrate, elementary sulfur. This suggests that the acidostability of the bacterium was enery-independent. The organism was subjected to osmotic shock with 0.75 M sucrose at 0 degrees C and then treated with snail intestinal juice in the p... | Acidostability of speroplasts prepared from Thiobacillus thiooxidans. Thiobacillus thiooxidans was acidostable even in the absence of its respiratory substrate, elementary sulfur. This suggests that the acidostability of the bacterium was enery-independent. The organism was subjected to osmotic shock with 0.75 M sucros... | [
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PMID:15529 | Effect of external factors on phototaxis of Chlamydomonas reinhardtii. II. Charbon dioxide, oxygen and pH. | 1. The effects of carbon dioxide, oxygen and pH on the inversion intensity of phototaxis of Chlamydomonas reinhardtii have been investigated. 2. With decreasing with CO2 tension the inversion intensity is decreased. 3. The gassing with CO2 can be substituted by hydrogencarbonate only to a small extent (20%). On the oth... | Effect of external factors on phototaxis of Chlamydomonas reinhardtii. II. Charbon dioxide, oxygen and pH. 1. The effects of carbon dioxide, oxygen and pH on the inversion intensity of phototaxis of Chlamydomonas reinhardtii have been investigated. 2. With decreasing with CO2 tension the inversion intensity is decrease... | [
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PMID:15530 | Penicillinase (beta-lactamase) formation by blue-green algae. | Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicellular species, Coccochloris elabens (strain 7003), and in a fil... | Penicillinase (beta-lactamase) formation by blue-green algae. Beta-Lactamase (penicillinase) activity was found in a number of strains of blue-green algea. In some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. Production and localization of beta-lactamase were studied in a unicell... | [
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PMID:15531 | Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate. | Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate : NADP+ L-oxidoreductase EC 1.1.1.49) isolated from Paracoccus denitrificans grown on glucose/nitrate exhibits both NAD+-and NADP+- linked activities. Both activities have a pH optimum of pH 9.6 (Glycine/NaOH buffer) and neither demonstrates a Mg2+ requirement. K... | Metabolic regulation of the glucose-6-phosphate dehydrogenase from Paracoccus denitrifcans grown on glucose/nitrate. Glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate : NADP+ L-oxidoreductase EC 1.1.1.49) isolated from Paracoccus denitrificans grown on glucose/nitrate exhibits both NAD+-and NADP+- linked activit... | [
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PMID:15532 | Cellular proteases increased in paramyxovirus (Sendai virus) carrier cells possibly responsible for enhanced formation of cowpox virus-specific cell surface antigen. | Cowpox virus (CPV) growth and its S-ag (cell surface antigen) formation in HVJ (Sendai virus) carrier cells pre-treated with Actinomycin D or Puromycin were not affected as much as those in parent cells. These suggest the different cellular functions of carrier cells. The activity of carrier cell extracts causing a ch... | Cellular proteases increased in paramyxovirus (Sendai virus) carrier cells possibly responsible for enhanced formation of cowpox virus-specific cell surface antigen. Cowpox virus (CPV) growth and its S-ag (cell surface antigen) formation in HVJ (Sendai virus) carrier cells pre-treated with Actinomycin D or Puromycin w... | [
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PMID:15535 | Overlay dentures. Philosophy and practice. II. | The practical stages in the construction of mandibular overlay dentures, with and without precision attachment anchors, are described and illustrated in detail. The importance of post-insertion maintenance, continuing effective oral hygiene and patient care, as a prerequisite for a successful overlay denture experience... | Overlay dentures. Philosophy and practice. II. The practical stages in the construction of mandibular overlay dentures, with and without precision attachment anchors, are described and illustrated in detail. The importance of post-insertion maintenance, continuing effective oral hygiene and patient care, as a prerequis... | [
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PMID:15536 | Fatty liver and kidney syndrome in chicks. II. Biochemical role of biotin. | The role of biotin-dependent enzymes in the fatty liver and kidney syndrome of young chicks was studied. Under conditions of a marginal deficiency of dietary biotin, the level of biotin in the liver has differing effects on the activities of two biotin-dependent enzymes, pyruvate carboxylase and acetyl-CoA carboxylase.... | Fatty liver and kidney syndrome in chicks. II. Biochemical role of biotin. The role of biotin-dependent enzymes in the fatty liver and kidney syndrome of young chicks was studied. Under conditions of a marginal deficiency of dietary biotin, the level of biotin in the liver has differing effects on the activities of two... | [
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PMID:15537 | Changes in plasma enzyme concentrations following intramuscular injections and gastroscopy. | The effects of intramuscular injections on plasma creatine kinase (CK), aspartate amino-transferase, lactate dehydrogenase, and hydroxybutyrate dehydrogenase concentrations were examined in 19 patients given intramuscular premedication for gastroscopy, and 18 patients given other intramuscular injections. Only CK conce... | Changes in plasma enzyme concentrations following intramuscular injections and gastroscopy. The effects of intramuscular injections on plasma creatine kinase (CK), aspartate amino-transferase, lactate dehydrogenase, and hydroxybutyrate dehydrogenase concentrations were examined in 19 patients given intramuscular premed... | [
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PMID:15538 | Effects of changes in pH of bath fluid on isolated guinea pig ileum preparation. | The effects of changes in the pH of Tyrode's solution on the responses of isolated guinea pig ileum preparation to acetylcholine and histamine were studied. At higher levels of pH (increased alkalinity), the responses of the tissue to both acetylcholine and histamine were increased. At lower pH levels (increased acidit... | Effects of changes in pH of bath fluid on isolated guinea pig ileum preparation. The effects of changes in the pH of Tyrode's solution on the responses of isolated guinea pig ileum preparation to acetylcholine and histamine were studied. At higher levels of pH (increased alkalinity), the responses of the tissue to both... | [
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PMID:15539 | Glycosidases from the culture medium of Physarum polycephalum. | Eight exo-glycosidase activities were detected in the axenic culture medium of the myxomycete, Physarum polycephalum. The secretion of each enzyme examined followed the growth curve and continued during the stationary phase after the cessation of growth. Two or more forms of each enzyme were detected after electrophore... | Glycosidases from the culture medium of Physarum polycephalum. Eight exo-glycosidase activities were detected in the axenic culture medium of the myxomycete, Physarum polycephalum. The secretion of each enzyme examined followed the growth curve and continued during the stationary phase after the cessation of growth. Tw... | [
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PMID:15540 | A pulse-radiolysis study of the catalytic mechanism of the iron-containing superoxide dismutase from Photobacterium leiognathi. | The mechanism of the enzymic reaction of an iron-containing superoxide dismutase purified from the marine bacterium Photobacterium leiognathi was studied by using pulse radiolysis. Measurements of activity were done with two different preparations of enzyme containing either 1.6 or 1.15 g-atom of iron/mol. In both case... | A pulse-radiolysis study of the catalytic mechanism of the iron-containing superoxide dismutase from Photobacterium leiognathi. The mechanism of the enzymic reaction of an iron-containing superoxide dismutase purified from the marine bacterium Photobacterium leiognathi was studied by using pulse radiolysis. Measurement... | [
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PMID:15541 | A study of the ionic properties of the essential histidine residue of yeast alcohol dehydrogenase in complexes of the enzyme with its coenzymes and substrates. | 1. Initial-rate studies of the reduction of acetaldehyde by NADH, catalysed by yeast alcohol dehydrogenase, were performed at pH 4.9 and 9.9, in various buffers, at 25 degrees C. The results are discussed in terms of the mechanism previously proposed for the pH range 5.9-8.9 [Dickenson & Dickinson (1975) Biochem. J... | A study of the ionic properties of the essential histidine residue of yeast alcohol dehydrogenase in complexes of the enzyme with its coenzymes and substrates. 1. Initial-rate studies of the reduction of acetaldehyde by NADH, catalysed by yeast alcohol dehydrogenase, were performed at pH 4.9 and 9.9, in various buffers... | [
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PMID:15542 | The use of gadolinium as a probe in the Fc region of a homogeneous anti-(type-III pneumococcal polysaccharide) antibody. | The binding of gadolinium [Gd(III)] to a homogeneous rabbit anti-(type-III pneumococcal polysaccharide) IgG (immunoglobulin G) and its Fab (N-terminal half of heavy and light chain) and Fc (C-terminal half of heavy-chain dimer) fragments was demonstrated by measurements of solvent-water proton relaxation rates in the a... | The use of gadolinium as a probe in the Fc region of a homogeneous anti-(type-III pneumococcal polysaccharide) antibody. The binding of gadolinium [Gd(III)] to a homogeneous rabbit anti-(type-III pneumococcal polysaccharide) IgG (immunoglobulin G) and its Fab (N-terminal half of heavy and light chain) and Fc (C-termina... | [
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PMID:15543 | Guanosine cyclic monophosphate-dependent protein kinase from foetal calf heart. Purification, general properties and catalytic subunit. | Cyclic GMP-dependent protein kinase was purified from foetal calf hearts, and its general properties and subunit structure were studied. The enzyme was purified over 900-fold from the heart extract by pH 5.3-isoelectric precipitation, DEAE-cellulose chromatography, Sephadex G-200 filtration and hydroxyapatite treatment... | Guanosine cyclic monophosphate-dependent protein kinase from foetal calf heart. Purification, general properties and catalytic subunit. Cyclic GMP-dependent protein kinase was purified from foetal calf hearts, and its general properties and subunit structure were studied. The enzyme was purified over 900-fold from the ... | [
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PMID:15544 | Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b. | 1. A three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from Methylosinus trichosporium. 2. The components are (i) a soluble CO-binding cytochrome c, (ii) a copper-containing protein and (iii) a small protein; the mol. wts. are 13 000, 47 000 and 9400 respectively... | Purification and properties of the methane mono-oxygenase enzyme system from Methylosinus trichosporium OB3b. 1. A three-component enzyme system that catalyses the oxidation of methane to methanol has been highly purified from Methylosinus trichosporium. 2. The components are (i) a soluble CO-binding cytochrome c, (ii)... | [
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PMID:15545 | Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans. | 1. Extracts of amine-grown Pseudomonas aminovorans contained a particle-bound N-methylglutamate dehydrogenase (EC 1.5.99.5). The enzyme was not present in succinate-grown cells, and activity appeared before growth began in succinate-grown cells which had been transferred to methylamine growth medium. 2. Membrane-contai... | Solubilization, partial purification and properties of N-methylglutamate dehydrogenase from Pseudomonas aminovorans. 1. Extracts of amine-grown Pseudomonas aminovorans contained a particle-bound N-methylglutamate dehydrogenase (EC 1.5.99.5). The enzyme was not present in succinate-grown cells, and activity appeared bef... | [
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PMID:15546 | Isolation and characterization of sheep pepsin. | Sheep pepsin was isolated (approx. 120-fold purification) from aqueous abomasal homogenates by (1) pH fractionation, (2) chromatography on Sepharose 4B-poly-L-lysine columns and (3) gel filtration on Sephadex G-100. The enzyme had mol.wt. approx. 34000, N-terminal valine and C-terminal alanine. The amino acid compositi... | Isolation and characterization of sheep pepsin. Sheep pepsin was isolated (approx. 120-fold purification) from aqueous abomasal homogenates by (1) pH fractionation, (2) chromatography on Sepharose 4B-poly-L-lysine columns and (3) gel filtration on Sephadex G-100. The enzyme had mol.wt. approx. 34000, N-terminal valine ... | [
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PMID:15547 | Properties of rat liver microsomal stearoyl-coenzyme A desaturase. | 1. Rat liver microsomal stearoyl-CoA desaturase activity was shown to be stimulated by both bovine serum albumin and a basic cytoplasmic protein from rat liver. 2. Partially purified desaturase is unaffected by either of these two proteins. 3. Bovine serum albumin appears to exert its effect on the crude system by prot... | Properties of rat liver microsomal stearoyl-coenzyme A desaturase. 1. Rat liver microsomal stearoyl-CoA desaturase activity was shown to be stimulated by both bovine serum albumin and a basic cytoplasmic protein from rat liver. 2. Partially purified desaturase is unaffected by either of these two proteins. 3. Bovine se... | [
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PMID:15548 | The isolation and partial characterization of the major glycoprotein (LGP-I) from the articular lubricating fraction from bovine synovial fluid. | The articular lubricating fraction from bovine synovial fluid was prepared by repeated fractionation in three consecutive CsCl density gradients to remove completely traces of hyaluronic acid. The major glycoprotein consituent (LGP-I) was then isolated by repeated gel-permeation chromatography. The yield of the LGP-I c... | The isolation and partial characterization of the major glycoprotein (LGP-I) from the articular lubricating fraction from bovine synovial fluid. The articular lubricating fraction from bovine synovial fluid was prepared by repeated fractionation in three consecutive CsCl density gradients to remove completely traces of... | [
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PMID:15549 | Purification and properties of an alpha-D-galactoside galactohydrolase from the seeds of Trifolium repens (white clover). | Five alpha-D-galactosidases (alpha-D-galactoside galactohydrolase; EC 3.2.1.22) have been identified by chromatography and polyacrylamide-disc-gel electrophoresis in the germinated seeds of Trifolium repens (white clover). alpha-Galactosidase I has been purified to homogeneity with an approx. 2000-fold increase in spec... | Purification and properties of an alpha-D-galactoside galactohydrolase from the seeds of Trifolium repens (white clover). Five alpha-D-galactosidases (alpha-D-galactoside galactohydrolase; EC 3.2.1.22) have been identified by chromatography and polyacrylamide-disc-gel electrophoresis in the germinated seeds of Trifoliu... | [
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PMID:15550 | An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase. | 1. 6-phosphogluconate dehydrogenase from sheep liver has been purified 350-fold by affinity chromatography with a final specific activity of 18 micronmol of NADP+/reduced min per mg of protein and an overall yield of greater than 40%. 2. A systematic investigation of potential ligands has been carried out: these includ... | An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase. 1. 6-phosphogluconate dehydrogenase from sheep liver has been purified 350-fold by affinity chromatography with a final specific activity of 18 micronmol of NAD... | [
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PMID:15551 | Large fragments of human serum albumin. | Large fragments of human serum albumin were produced by treatment of the native protein with pepsin at pH3.5. Published sequences of human albumin [Behrens, Spiekerman & Brown (1975) Fed. Proc. Fed. Am. Soc. Exp. Biol. 34, 591; Meloun, Moravek & Kostka (1975) FEBSLett.58, 134-137]were used to locate the fragmen... | Large fragments of human serum albumin. Large fragments of human serum albumin were produced by treatment of the native protein with pepsin at pH3.5. Published sequences of human albumin [Behrens, Spiekerman & Brown (1975) Fed. Proc. Fed. Am. Soc. Exp. Biol. 34, 591; Meloun, Moravek & Kostka (1975) FEBSLett.58,... | [
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PMID:15552 | Modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity. | Methyl methanethiosulphonate was used to produce a modification of the essential thiol group in lactate dehydrogenase which leaves the enzyme catalytically active. Methyl methanethiosulphonate produced a progressive inhibition of enzyme activity, with 2mM-pyruvate and 0.14mM-NADH as substrates, which ceased once the en... | Modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity. Methyl methanethiosulphonate was used to produce a modification of the essential thiol group in lactate dehydrogenase which leaves the enzyme catalytically active. Methyl methanethiosu... | [
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PMID:15553 | Studies on the soluble and membrane-bound amino acid 2-naphthylamidases in pig and human epidermis. | 1. Membrane-bound (particulate) and soluble amino acid 2-naphthylamidases (EC 3.5.1.-) were present in subcellular fractions of epidermis from pig and human. 2. The particulate enzymes exhibited Michaelis-Menten kinetics, with Km 5.1x10(-5) (pig) and Km 7.3x10(-5)M (human) for the substrate L-leucine 2-naphthylamide. T... | Studies on the soluble and membrane-bound amino acid 2-naphthylamidases in pig and human epidermis. 1. Membrane-bound (particulate) and soluble amino acid 2-naphthylamidases (EC 3.5.1.-) were present in subcellular fractions of epidermis from pig and human. 2. The particulate enzymes exhibited Michaelis-Menten kinetics... | [
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PMID:15554 | Self-association of alpha-chymotrypsin at low ionic strength in the vicinity of its pH optimum. | The self-association of alpha-chymotrypsin and its di-isopropyl phosphoryl derivative in in I0.03 sodium phophate buffer, pH7,9, was investigated by velocity sedimentation, equilibrium sedimentation and difference gel chromatography. No differences between the native and chemically modified enzyme were observed in the ... | Self-association of alpha-chymotrypsin at low ionic strength in the vicinity of its pH optimum. The self-association of alpha-chymotrypsin and its di-isopropyl phosphoryl derivative in in I0.03 sodium phophate buffer, pH7,9, was investigated by velocity sedimentation, equilibrium sedimentation and difference gel chroma... | [
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PMID:15555 | Proton uptake linked to the 3-deoxy-2-oxo-d-gluconate-transport system of Escherichia coli. | Genetic and kinetic evidence is presented to show that the carrier-mediated uptake of the anionic sugars 3-deoxy-2-oxo-D-gluconate and D-glucuronate by Escherichia coli involves the concomitant transport of protons. | Proton uptake linked to the 3-deoxy-2-oxo-d-gluconate-transport system of Escherichia coli. Genetic and kinetic evidence is presented to show that the carrier-mediated uptake of the anionic sugars 3-deoxy-2-oxo-D-gluconate and D-glucuronate by Escherichia coli involves the concomitant transport of protons. | [
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PMID:15556 | An oligomycin-resistant adenosine triphosphatase and its effects on cellular growth, mitochondrial oxidative phosphorylation and respiratory proton translocation in Saccharomyces cerevisiae. | Mutations at the OLI 1 or OLI 2 loci of mitochondria DNA in Saccharomyces cerevisiae are associated with a diminished growth rate in nutritionally suboptimal cultures supplemented with an oxidizable carbon source. In the case of mutant OR146(OLI1) there is a 35% loss of mitochondrial protein during fractionation in vit... | An oligomycin-resistant adenosine triphosphatase and its effects on cellular growth, mitochondrial oxidative phosphorylation and respiratory proton translocation in Saccharomyces cerevisiae. Mutations at the OLI 1 or OLI 2 loci of mitochondria DNA in Saccharomyces cerevisiae are associated with a diminished growth rate... | [
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PMID:15557 | The metabolism of benzyl isothiocyanate and its cysteine conjugate. | 1. The corresponding cysteine conjugate was formed when the GSH (reduced glutathione) or cysteinylglycine conjugates of benzyl isothiocyanate were incubated with rat liver or kidney homogenates. When the cysteine conjugate of benzyl isothiocyanate was similarly incubated in the presence of acetyl-CoA, the corresponding... | The metabolism of benzyl isothiocyanate and its cysteine conjugate. 1. The corresponding cysteine conjugate was formed when the GSH (reduced glutathione) or cysteinylglycine conjugates of benzyl isothiocyanate were incubated with rat liver or kidney homogenates. When the cysteine conjugate of benzyl isothiocyanate was ... | [
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PMID:15558 | Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. | 1. Strains of Escherichia coli K12 were made that are unable to assimilate glucose by the phosphotransferase system, since they lack the glucose-specific components specified by the genes ptsG and ptsM. 2. Derivative organisms lacking the methyl galactoside or galactose-specific transport system were examined for their... | Transport of galactose, glucose and their molecular analogues by Escherichia coli K12. 1. Strains of Escherichia coli K12 were made that are unable to assimilate glucose by the phosphotransferase system, since they lack the glucose-specific components specified by the genes ptsG and ptsM. 2. Derivative organisms lackin... | [
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PMID:15559 | Occurrence of ornithine decarboxylase and polyamines in cartilage. | The activity of ornithine decarboxylase was investigated in cartilage from chick embryos, rabbits, rats and human foetuses. The enzyme activity in these cartilages was of the same order as the detected in other body tissues. Ornithine decarboxylase activity in chick-embryo cartilage and liver was the same when compared... | Occurrence of ornithine decarboxylase and polyamines in cartilage. The activity of ornithine decarboxylase was investigated in cartilage from chick embryos, rabbits, rats and human foetuses. The enzyme activity in these cartilages was of the same order as the detected in other body tissues. Ornithine decarboxylase acti... | [
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PMID:15560 | Binding of magnesium ions to cell walls of Bacillus subtilis W23 containing teichoic acid or teichuronic acid. | When grown in a chemostat under various nutritional conditions, cells of Bacillus subtilis W23 produce walls containing teichoic acid or teichuronic acid. The binding of Mg2+ to these walls and to the isolated anionic polymers in solution was measured by equilibrium dialysis. In solution the ribitol teichoic acid bound... | Binding of magnesium ions to cell walls of Bacillus subtilis W23 containing teichoic acid or teichuronic acid. When grown in a chemostat under various nutritional conditions, cells of Bacillus subtilis W23 produce walls containing teichoic acid or teichuronic acid. The binding of Mg2+ to these walls and to the isolated... | [
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PMID:15568 | [The effect of the rauwolfia-alkaloids ajmaline, rescinnamine and reserpine on the catecholamine contents of the heart (author's transl)]. | The effect of three rauwolfia-alkaloids and two semi-synthetic derivatives on the catecholamine content of the hearts of guinea pigs was examined. The substances were: ajamaline, N-methyl-ajamaline, N-n-propylajmaline, rescinnamine and reserpine. The presence of catecholamines in isolated atria was tested with tyramine... | [The effect of the rauwolfia-alkaloids ajmaline, rescinnamine and reserpine on the catecholamine contents of the heart (author's transl)]. The effect of three rauwolfia-alkaloids and two semi-synthetic derivatives on the catecholamine content of the hearts of guinea pigs was examined. The substances were: ajamaline, N-... | [
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PMID:15569 | [Action of partially cyclic and ring methylated nikethamide analogs. Structure-activity relationships of analeptics of the nicethamide type]. | Investigation concerning the respiratory-analeptical effect of partially cyclic and ring methylated nicethamide analogues are reported. The respiration of the rabbit determined by the mask method, served as pharmacological model. | [Action of partially cyclic and ring methylated nikethamide analogs. Structure-activity relationships of analeptics of the nicethamide type]. Investigation concerning the respiratory-analeptical effect of partially cyclic and ring methylated nicethamide analogues are reported. The respiration of the rabbit determined b... | [
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PMID:15570 | [The effects of barbiturates on the embryonic growth of seedling of lepidium sativum L (author's transl)]. | The influence of some important barbiturates on the capacity to stimulate or to stop the growth of seedling of Lepidium sativum L. was tested and the results statistically evaluated. Phenobarbital, cyclobarbital and pentobarbitial show a significant stimulating effect on growth between 10(-2) and 10(-4) moles/l. All ba... | [The effects of barbiturates on the embryonic growth of seedling of lepidium sativum L (author's transl)]. The influence of some important barbiturates on the capacity to stimulate or to stop the growth of seedling of Lepidium sativum L. was tested and the results statistically evaluated. Phenobarbital, cyclobarbital a... | [
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PMID:15571 | High performance liquid chromatography in research of pharmacokinetics and metabolism. | High performance liquid chromatography in connection with monochromatic UV-detection has proved to be a powerful tool for separation and quantitative determination of drugs and their metabolites in body fluids. Serum samples from volunteers medicated with the psychotropic drugs fosazepam and nomifensine are analysed by... | High performance liquid chromatography in research of pharmacokinetics and metabolism. High performance liquid chromatography in connection with monochromatic UV-detection has proved to be a powerful tool for separation and quantitative determination of drugs and their metabolites in body fluids. Serum samples from vol... | [
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PMID:15572 | [Compound with bronchospasmolytical activity from the chemical class of beta-phenylethyl-aminoalkyl-xanthines (author's transl)]. | 1. 7-(3-[2-(3,5-Dihydroxyphenyl)-2-hydroxy-ethylamino]-propyl)-theophylline (reproterol, D 1959, Bronchospasmin) was developed as a bronchospasmolytic agent from the structure class of the phenylethyl-aminoalkyl-xanthines. 2. The pharmacological effects characterize reproterol as a bronchospasmolytic with preferential ... | [Compound with bronchospasmolytical activity from the chemical class of beta-phenylethyl-aminoalkyl-xanthines (author's transl)]. 1. 7-(3-[2-(3,5-Dihydroxyphenyl)-2-hydroxy-ethylamino]-propyl)-theophylline (reproterol, D 1959, Bronchospasmin) was developed as a bronchospasmolytic agent from the structure class of the p... | [
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PMID:15581 | Purification and characterization of guinea-pig epidermal acid phosphatase. | Guinea-pig epidermal acid phosphatase has been purified approximately 120-fold by a procedure including acid treatment, CM-cellulose and DEAE-cellulose chromatography, and gel filtration on Sephadex G-100. The enzyme had a pH optimum at 5-0 and the optimal temperature for activity was approximately 50 degrees C. The en... | Purification and characterization of guinea-pig epidermal acid phosphatase. Guinea-pig epidermal acid phosphatase has been purified approximately 120-fold by a procedure including acid treatment, CM-cellulose and DEAE-cellulose chromatography, and gel filtration on Sephadex G-100. The enzyme had a pH optimum at 5-0 and... | [
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PMID:15582 | Neutrophil function in bone marrow transplant recipients. | The neutrophil function of seven patients receiving allogeneic bone marrow transplantion was studied. Five of the patients had been transplanted for aplastic anaemia and two for acute leukaemia. Determinations were made of neutrophil phagocytosis, chemotaxis, random migration, and microbicidal activity for Candida albi... | Neutrophil function in bone marrow transplant recipients. The neutrophil function of seven patients receiving allogeneic bone marrow transplantion was studied. Five of the patients had been transplanted for aplastic anaemia and two for acute leukaemia. Determinations were made of neutrophil phagocytosis, chemotaxis, ra... | [
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PMID:15583 | Effect of fat supplementation on voluntary food intake and rumen metabolism in sheep. | 1. In an experiment in which a high-fat supplement was given in the dry form to lambs offered dried grass ad lib., both the voluntary intake and digestibility of the dried grass were reduced. When the high-fat supplement was given in a liquid suspension so that the rumen was by-passed, the voluntary intake and digestib... | Effect of fat supplementation on voluntary food intake and rumen metabolism in sheep. 1. In an experiment in which a high-fat supplement was given in the dry form to lambs offered dried grass ad lib., both the voluntary intake and digestibility of the dried grass were reduced. When the high-fat supplement was given in ... | [
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PMID:15584 | Fluorine-19 nuclear magnetic resonance studies of effects of ligands on trifluoroacetonylated supernatant aspartate transaminase. | The selective reaction of Cys-45 and -82, on the one hand, and Cys-390, on the other, with 3-bromo-1,1,1-trifluoropropanone allows for the probing of these regions of aspartate transaminase in the absence and in the presence of enzymatic ligands by 19F nuclear magnetic resonance (NMR). The 19F chemical shifts of the re... | Fluorine-19 nuclear magnetic resonance studies of effects of ligands on trifluoroacetonylated supernatant aspartate transaminase. The selective reaction of Cys-45 and -82, on the one hand, and Cys-390, on the other, with 3-bromo-1,1,1-trifluoropropanone allows for the probing of these regions of aspartate transaminase ... | [
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PMID:15585 | Evidence for a critical glutamyl and an aspartyl residue in the function of pig heart diphosphopyridine nucleotide dependent isocitrate dehydrogenase. | The pH dependence of the maximum velocity of the reaction catalyzed by diphosphopyridine nucleotide (DPN) dependent isocitrate dehydrogenase indicates the requirement for the basic form of an ionizable group in the enzyme-substrate complex with a pK of 6.6. This pK is unaltered from 10 to 33 degrees C, suggesting the i... | Evidence for a critical glutamyl and an aspartyl residue in the function of pig heart diphosphopyridine nucleotide dependent isocitrate dehydrogenase. The pH dependence of the maximum velocity of the reaction catalyzed by diphosphopyridine nucleotide (DPN) dependent isocitrate dehydrogenase indicates the requirement fo... | [
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PMID:15586 | Binding rates, O--S substitution effects, and the pH dependence of chymotrypsin reactions. | The pH dependence for acylation of alpha-chymotrypsin by N-acetyltryptophan p-nitrophenyl-, p-nitrothiophenyl-, ethyl-, and thiolethyl esters has been studied by the stopped-flow technique. Values for the acylation rate constant, k2, and the binding constant, KS, were obtained by using measurements of phenolate release... | Binding rates, O--S substitution effects, and the pH dependence of chymotrypsin reactions. The pH dependence for acylation of alpha-chymotrypsin by N-acetyltryptophan p-nitrophenyl-, p-nitrothiophenyl-, ethyl-, and thiolethyl esters has been studied by the stopped-flow technique. Values for the acylation rate constant,... | [
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PMID:15587 | Comparison of the biosynthetic and biodegradative ornithine decarboxylases of Escherichia coli. | Biosynthetic ornithine decarboxylase was purified 4300-fold from Escherichia coli to a purity of approximately 85% as judged by polyacrylamide gel electrophoresis. The enzyme showed hyperbolic kinetics with a Km of 5.6 mM for ornithine and 1.0 micronM for pyridoxal phosphate and it was competitively inhibited by putres... | Comparison of the biosynthetic and biodegradative ornithine decarboxylases of Escherichia coli. Biosynthetic ornithine decarboxylase was purified 4300-fold from Escherichia coli to a purity of approximately 85% as judged by polyacrylamide gel electrophoresis. The enzyme showed hyperbolic kinetics with a Km of 5.6 mM fo... | [
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PMID:15588 | An investigation of heavy meromyosin-ADP binding equilibria by proton release measurements. | The interaction of magnesium-ADP with skeletal muscle heavy meromyosin has been studied by measuring the accompanying release of protons. Total pH changes of the order of 0.03 were involved, and measurements were performed with a discrimination of some ten-thousandths of a pH unit. At pH 8.0 and 25 degrees C about 0.5 ... | An investigation of heavy meromyosin-ADP binding equilibria by proton release measurements. The interaction of magnesium-ADP with skeletal muscle heavy meromyosin has been studied by measuring the accompanying release of protons. Total pH changes of the order of 0.03 were involved, and measurements were performed with ... | [
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PMID:15589 | Effects of neurotoxins (veratridine, sea anemone toxin, tetrodotoxin) on transmitter accumulation and release by nerve terminals in vitro. | Two of the tree toxic compounds used in this work, veratridine and the sea anemone toxin, provoke neurotransmitter release from synaptosomes; the third one, tetrodotoxin, prevents the action of both veratridine and the sea anemone toxin. The half-maximum effects of veratridine and sea anemone toxin actions on synaptoso... | Effects of neurotoxins (veratridine, sea anemone toxin, tetrodotoxin) on transmitter accumulation and release by nerve terminals in vitro. Two of the tree toxic compounds used in this work, veratridine and the sea anemone toxin, provoke neurotransmitter release from synaptosomes; the third one, tetrodotoxin, prevents t... | [
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PMID:15590 | Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. | A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chromatography, Sephadex G-200 gel filtration, isoelectric focusing, and sodium dodecyl sulfate gel electrophoresis. The... | Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. A plasminogen activator secreted by cultured human pancreatic carcinoma (Mia PaCa-2) cells has been purified to apparent homogeneity by procedures including Sepharose-L-arginine methyl ester affinity chro... | [
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PMID:15591 | Correlation of redox levels of component electron carriers with total electron flux in an electron-transport system. P-700 and the photoreduction of NADP+ in chloroplast fragments. | A mathematical analysis is described which measures the effects of actinic light intensity and concentration of an artificial electron donor on the steady-state light-induced redox level of a reaction-center pigment (e.g. P-700) and on the overall light-induced electron flux (e.g. reduction of NADP+). The analysis led ... | Correlation of redox levels of component electron carriers with total electron flux in an electron-transport system. P-700 and the photoreduction of NADP+ in chloroplast fragments. A mathematical analysis is described which measures the effects of actinic light intensity and concentration of an artificial electron dono... | [
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PMID:15592 | The effect of physiologically occurring cations upon aequorin light emission. Determination of the binding constants. | 1. The effect of K+, Na+, Mg2+ and pH upon the rate of aequorin utilization has been investigated in the presence of Ca2+. 2. The aequorin light emission in a medium simulating the in vivo cationic conditions for barnacle muscle fibres indicates that two Ca2+ are apparently involved in this process for free calcium con... | The effect of physiologically occurring cations upon aequorin light emission. Determination of the binding constants. 1. The effect of K+, Na+, Mg2+ and pH upon the rate of aequorin utilization has been investigated in the presence of Ca2+. 2. The aequorin light emission in a medium simulating the in vivo cationic cond... | [
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PMID:15593 | Pathways of silicomolybdate photoreduction and associated photophosphorylation in tobacco chloroplasts. | Three sites of silicomolybdate reduction in the electron transport chain of isolated tobacco chloroplasts are described. The relative participation of these sites is greatly influenced by the particular reaction conditions. One site (the only site when the reaction medium contains high concentrations of bovine serum al... | Pathways of silicomolybdate photoreduction and associated photophosphorylation in tobacco chloroplasts. Three sites of silicomolybdate reduction in the electron transport chain of isolated tobacco chloroplasts are described. The relative participation of these sites is greatly influenced by the particular reaction cond... | [
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PMID:15594 | Adenine nucleotide transport in sonic submitochondrial particles. Kinetic properties and binding of specific inhibitors. | 1. A procedure for preparation of sonic submitochondrial particles competent for adenine nucleotide transport is described. ADP or ATP transport was assayed, in the presence of oligomycin, in a saline medium made of 0.125 M KCl, 1 mM EDTA, 10 mM 4-morpholinopropane sulfonic acid buffer, pH 6.5. 2. Sonic particles trans... | Adenine nucleotide transport in sonic submitochondrial particles. Kinetic properties and binding of specific inhibitors. 1. A procedure for preparation of sonic submitochondrial particles competent for adenine nucleotide transport is described. ADP or ATP transport was assayed, in the presence of oligomycin, in a salin... | [
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PMID:15595 | Bioflavonoid regulation of ATPase and hexokinase activity in Ehrlich ascites cell mitochondria. | (1) The mitochondrial ATPase (EC 3.6.1.3) Ehrlich ascites cell mitochondria, was inhibited by D-glucose under physiological concentrations of ATP. The generation of ADP by the mitochondrial bound hexokinase, seems to be the reason for the D-glucose inhibitory effect. Reversal of the inhibitory effect of ADP on Ehrlich ... | Bioflavonoid regulation of ATPase and hexokinase activity in Ehrlich ascites cell mitochondria. (1) The mitochondrial ATPase (EC 3.6.1.3) Ehrlich ascites cell mitochondria, was inhibited by D-glucose under physiological concentrations of ATP. The generation of ADP by the mitochondrial bound hexokinase, seems to be the ... | [
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PMID:15596 | Phosphate transport in Micrococcus lysodeikticus. | Phosphate accumulates in Micrococcus lysodeikticus cells against a concentration gradient, by an energy-dependent process. The phosphate transport is derepressed during phosphate deprivation. The depression process is inhibited by chloramphenicol. The apparent Km of phosphate transport is 4.3 micronM. The activation e... | Phosphate transport in Micrococcus lysodeikticus. Phosphate accumulates in Micrococcus lysodeikticus cells against a concentration gradient, by an energy-dependent process. The phosphate transport is derepressed during phosphate deprivation. The depression process is inhibited by chloramphenicol. The apparent Km of pho... | [
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PMID:15597 | Preparation of renal cortex basal-lateral and bursh border membranes. Localization of adenylate cyclase and guanylate cyclase activities. | Luminal brush border and contraluminal basal-lateral segments of the plasma membrane from the same kidney cortex were prepared. The brush border membrane preparation was enriched in trehalase and gamma-glutamyltranspeptidase, whereas the basal-lateral membrane preparation was enriched in (Na+ + K+1)-ATPase. However, th... | Preparation of renal cortex basal-lateral and bursh border membranes. Localization of adenylate cyclase and guanylate cyclase activities. Luminal brush border and contraluminal basal-lateral segments of the plasma membrane from the same kidney cortex were prepared. The brush border membrane preparation was enriched in ... | [
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PMID:15598 | Effect of the medium pH and the cell pH upon the kinetical parameters of phosphate uptake by yeast. | 1. Both the maximum rate of phosphate uptake and the Km depend upon the pH of the medium in a complex way. 2. The effect of medium pH upon the maximum rate of uptake is mainly indirect and is correlated with changes in cell pH. 3. The Km is affected by the medium pH both directly via an apparent competitive inhibition ... | Effect of the medium pH and the cell pH upon the kinetical parameters of phosphate uptake by yeast. 1. Both the maximum rate of phosphate uptake and the Km depend upon the pH of the medium in a complex way. 2. The effect of medium pH upon the maximum rate of uptake is mainly indirect and is correlated with changes in c... | [
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PMID:15599 | Pyrrolo(1,4)benzodiazepine antitumor antibiotics. In vitro interaction of anthramycin, sibiromycin and tomaymycin with DNA using specifically radiolabelled molecules. | Anthramycin, tomaymycin and sibiromycin are pyrrolo(1,4)benzodiazepine antitumor antibiotics. These compounds react with DNA and other guanine-containing polydeoxynucleotides to form covalently bound antibiotic - polydeoxynucleotide complexes. Experiments utilizing radiolabelled antibiotics have led to the following co... | Pyrrolo(1,4)benzodiazepine antitumor antibiotics. In vitro interaction of anthramycin, sibiromycin and tomaymycin with DNA using specifically radiolabelled molecules. Anthramycin, tomaymycin and sibiromycin are pyrrolo(1,4)benzodiazepine antitumor antibiotics. These compounds react with DNA and other guanine-containing... | [
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PMID:15600 | Reactive carriers of immobilized compounds. | Sphericanl macroporous reactive carriers capable of forming covalent bonds with amino acids and proteins were prepared by the suspension copolymerization of 2-hydroxyethyl methacrylate, ethylene dimethacrylate and p-nitrophenyl esters of methacrylic acid and methacryloyl derivatives of glycine, beta-alanine and epsilon... | Reactive carriers of immobilized compounds. Sphericanl macroporous reactive carriers capable of forming covalent bonds with amino acids and proteins were prepared by the suspension copolymerization of 2-hydroxyethyl methacrylate, ethylene dimethacrylate and p-nitrophenyl esters of methacrylic acid and methacryloyl deri... | [
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PMID:15601 | Purification of the (Na+ + K+)-adenosine triphosphatase from human renal tissue. | (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was purified from human cadaver renal tissue and exhibited a linear reaction rate with time. 100 g of whole kidney would yield 1--3.5 mg protein with a specific activity of 50--200 mol - kg-1 - h-1 for (Na+ + K+)-ATPase. The preparation was completely inhibited by 10... | Purification of the (Na+ + K+)-adenosine triphosphatase from human renal tissue. (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) was purified from human cadaver renal tissue and exhibited a linear reaction rate with time. 100 g of whole kidney would yield 1--3.5 mg protein with a specific activity of 50--200 mol -... | [
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PMID:15602 | Purification and crystallization of NADP+-specific isocitrate dehydrogenase from Escherichia coli using polyethylene glycol. | A simple and rapid method is presented for purifying the NADP+-dependent isocitrate dehydrogenase (threo-DS-isocitrate:NADP+ oxidoreductase (decarboxylating), from Escherichia coli, which relies on fractionation of the enzyme with polyethylene glycol. The shortened preparation results in a 32% relative recovery of puri... | Purification and crystallization of NADP+-specific isocitrate dehydrogenase from Escherichia coli using polyethylene glycol. A simple and rapid method is presented for purifying the NADP+-dependent isocitrate dehydrogenase (threo-DS-isocitrate:NADP+ oxidoreductase (decarboxylating), from Escherichia coli, which relies ... | [
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PMID:15603 | Glyceraldehyde-3-phosphate dehydrogenase of Scenedesmus obliquus. Effects of dithiothreitol and nucleotide on coenzyme specificity. | NADH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.--) of the photosynthetic alga Scenedesmus obliquus is converted to an NADPH specific form by incubation with dithiothreitol. The change in nucleotide specificity is accompanied by a reduction in the molecular weight of the enzyme from 550 000 to 140 000... | Glyceraldehyde-3-phosphate dehydrogenase of Scenedesmus obliquus. Effects of dithiothreitol and nucleotide on coenzyme specificity. NADH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.--) of the photosynthetic alga Scenedesmus obliquus is converted to an NADPH specific form by incubation with dithiothreit... | [
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PMID:15604 | Enzymic oxidation of 3-hydroxyxanthine to 3-hydroxyuric acid. | 1. Bovine milk xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2) oxidises 3-hydroxyxanthine slowly to 3-hydroxyuric acid; the 1-methyl derivative of 3-hydroxyxanthine is attacked about twice as fast. 2. The pH optimum for the reaction of 2-hydroxyxanthine is near 5, i.e. the neutral form of this substrate i... | Enzymic oxidation of 3-hydroxyxanthine to 3-hydroxyuric acid. 1. Bovine milk xanthine oxidase (xanthine:oxygen oxidoreductase, EC 1.2.3.2) oxidises 3-hydroxyxanthine slowly to 3-hydroxyuric acid; the 1-methyl derivative of 3-hydroxyxanthine is attacked about twice as fast. 2. The pH optimum for the reaction of 2-hydrox... | [
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PMID:15605 | The transient-state kinetics of L-glutamate dehydrogenase. pH-dependence of the burst rate parameters. | The pH dependence of the initial transient velocity of NADPH production during the burst phase of the oxidative deamination of L-glutamate by L-glutamate dehydrogenase (L-glutamate : NAD(P)+ oxidoreductase (deaminating), EC 1.4.1.3) and NADP+ has been measured by stopped-flow spectrophotometry. These studies provide ev... | The transient-state kinetics of L-glutamate dehydrogenase. pH-dependence of the burst rate parameters. The pH dependence of the initial transient velocity of NADPH production during the burst phase of the oxidative deamination of L-glutamate by L-glutamate dehydrogenase (L-glutamate : NAD(P)+ oxidoreductase (deaminatin... | [
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PMID:15606 | Cyanide formation from histidine in Chlorella. A general reaction of aromatic amino acids catalyzed by amino acid oxidase systems. | The formation of HCN from D-histidine in Chlorella vulgaris extracts is shown to be due to the combined action of a soluble protein and a particulate component. Either horse-radish peroxidase (EC 1.11.1.7) or a metal ion with redox properties can be substituted for the particulate component. Ions of manganese and vanad... | Cyanide formation from histidine in Chlorella. A general reaction of aromatic amino acids catalyzed by amino acid oxidase systems. The formation of HCN from D-histidine in Chlorella vulgaris extracts is shown to be due to the combined action of a soluble protein and a particulate component. Either horse-radish peroxida... | [
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PMID:15607 | A D-amino acid oxidase from Chlorella vulgaris. | A procedure has been developed for the partial purification from Chlorella vulgaris of an enzyme which catalyzes the formation of HCN from D-histidine when supplemented with peroxidase of a metal with redox properties. Some properties of the enzyme are described. Evidence is presented that the catalytic activity for HC... | A D-amino acid oxidase from Chlorella vulgaris. A procedure has been developed for the partial purification from Chlorella vulgaris of an enzyme which catalyzes the formation of HCN from D-histidine when supplemented with peroxidase of a metal with redox properties. Some properties of the enzyme are described. Evidence... | [
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PMID:15608 | Allosteric and non-allosteric phosphofructokinases from Lactobacilli. Purification and properties of phosphofructokinases from L. plantarum and L. acidophilus. | Phosphofructokinase (ATP : D-fructose-6-phosphate 1 phosphotransferase, EC 2.7.1.11) from two different lactobacilli, Lactobacillus plantarum and Lactobacillus acidophilus were isolated and purified. Both enzymes have a molecular weight of 154 000 and consist of four subunits of identical size. Antisera from sheep immu... | Allosteric and non-allosteric phosphofructokinases from Lactobacilli. Purification and properties of phosphofructokinases from L. plantarum and L. acidophilus. Phosphofructokinase (ATP : D-fructose-6-phosphate 1 phosphotransferase, EC 2.7.1.11) from two different lactobacilli, Lactobacillus plantarum and Lactobacillus ... | [
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PMID:15609 | Inactivation and reactivation of liver phosphorylase b kinase. | When crude rat liver preparations were incubated at 30degrees C, a gradual loss of phosphorylase kinase (ATP:phosphorylase b phosphotransferase, EC 2.7.1.38) activity was observed. This inactivation was Mg2+ dependent and was partially inhibited by sodium fluoride. Addition of Mg2+ ATP to the liver preparations, at any... | Inactivation and reactivation of liver phosphorylase b kinase. When crude rat liver preparations were incubated at 30degrees C, a gradual loss of phosphorylase kinase (ATP:phosphorylase b phosphotransferase, EC 2.7.1.38) activity was observed. This inactivation was Mg2+ dependent and was partially inhibited by sodium f... | [
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PMID:15610 | Compensational phenomena in reactivation of dimethyl- and diethylphosphoryl butyrylcholinesterases. | The thermodynamic and kinetic parameters for spontaneous and oxime reactivation of dimethyl- and diethylphosphoryl butyrylcholinesterases (acylcholine acyl-hydrolase, EC 3.1.1.8) are reported. The enthalpy and entropy changes in both the binding (deltaH0 and deltaS0) and the dephosphorylation steps (deltaH* and deltaS*... | Compensational phenomena in reactivation of dimethyl- and diethylphosphoryl butyrylcholinesterases. The thermodynamic and kinetic parameters for spontaneous and oxime reactivation of dimethyl- and diethylphosphoryl butyrylcholinesterases (acylcholine acyl-hydrolase, EC 3.1.1.8) are reported. The enthalpy and entropy ch... | [
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PMID:15611 | Catalytic activity of Ntau-carboxymethylhistidine-12 ribonuclease: pH dependence. | The pH-dependence of RNAase A and of Ntau-carboxymethylhistidine-12-RNAase (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase) catalysis was studied. Apparent acid dissociation constants were obtained by least squares analysis of the kinetics data. These dissociation constants were compared with pKa values of model i... | Catalytic activity of Ntau-carboxymethylhistidine-12 ribonuclease: pH dependence. The pH-dependence of RNAase A and of Ntau-carboxymethylhistidine-12-RNAase (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase) catalysis was studied. Apparent acid dissociation constants were obtained by least squares analysis of the ki... | [
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PMID:15613 | The activator of cerebroside sulphatase. Binding studies with enzyme and substrate demonstrating the detergent function of the activator protein. | 1. Sulphatase A (cerebroside sulphatase) (EC 3.1.6.1.) and a 12-fold excess of its physiological activator protein were chromatographed together on Sephadex G-75. The elution buffer was the same as that used in the enzymic degradation of sulphatides. The two proteins were eluted in different peaks indicating that no st... | The activator of cerebroside sulphatase. Binding studies with enzyme and substrate demonstrating the detergent function of the activator protein. 1. Sulphatase A (cerebroside sulphatase) (EC 3.1.6.1.) and a 12-fold excess of its physiological activator protein were chromatographed together on Sephadex G-75. The elution... | [
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PMID:15615 | The effect of pre-incubation on trypsin kinetics at low pH. | A possible source of discrepancy between kinetic and spectroscopic studies of the active site ionizations in the enzyme trypsin (EC 3.4.21.4) could arise if a slow pH-dependent conformational change affected the rates at low pH. No such effect is observed within the time range of 1 min- 3 h when pre-incubation of tryps... | The effect of pre-incubation on trypsin kinetics at low pH. A possible source of discrepancy between kinetic and spectroscopic studies of the active site ionizations in the enzyme trypsin (EC 3.4.21.4) could arise if a slow pH-dependent conformational change affected the rates at low pH. No such effect is observed with... | [
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PMID:15616 | Physicochemical properties of alpha- and beta-fibrinogenases of Trimeresurus mucrosquamatus venom. | alpha- and beta-Fibrinogenases (EC 3.4.21.5) were purified from Trimeresurus mucrosquamatus venom by the technique of recycling chromatography. Both enzymes were single polypeptide chains and homogeneous as judged by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and ultracentrifugation. The sedimentat... | Physicochemical properties of alpha- and beta-fibrinogenases of Trimeresurus mucrosquamatus venom. alpha- and beta-Fibrinogenases (EC 3.4.21.5) were purified from Trimeresurus mucrosquamatus venom by the technique of recycling chromatography. Both enzymes were single polypeptide chains and homogeneous as judged by sodi... | [
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PMID:15617 | Application of trinitrophenylation for the measurement of alpha-amino residues resulting from peptic digestion. | A sensitive and precise method for the measurement of peptic activity on protein substrate is described. alpha-Amino residues formed by pepsin digestion are photometrically measured by comparing the absorbances of digested and nondigested material which has been trinitrophenylated. The usual problem of high reagent-bla... | Application of trinitrophenylation for the measurement of alpha-amino residues resulting from peptic digestion. A sensitive and precise method for the measurement of peptic activity on protein substrate is described. alpha-Amino residues formed by pepsin digestion are photometrically measured by comparing the absorbanc... | [
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PMID:15618 | Characterization of an adenylyl cyclase activity in particulate preparations from epimastigote forms of Trypanosoma cruzi. | Particulate preparations from epimastigote forms of Trypanosoma cruzi contain an adenylyl cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) which could be stored at --20 degree C and resisted 5 cycles of freezing and thawing over 10 days without significant loss of activity. The enzyme reaction strictly require... | Characterization of an adenylyl cyclase activity in particulate preparations from epimastigote forms of Trypanosoma cruzi. Particulate preparations from epimastigote forms of Trypanosoma cruzi contain an adenylyl cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) which could be stored at --20 degree C and resist... | [
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PMID:15619 | Activation of liver guanylate cyclase by bile salts and contaminants in crude secretin and pancreozymin preparations. | Crude preparations of secretin or pancreozymin increased and at higher concentrations decreased guanylate cyclase (GTP pyophosphate-lyase, EC 4.6.1.2) activity from soluble and particulate fractions of rat liver homogenates. Partially purified and synthetic secretin were without effect as was the biologically active oc... | Activation of liver guanylate cyclase by bile salts and contaminants in crude secretin and pancreozymin preparations. Crude preparations of secretin or pancreozymin increased and at higher concentrations decreased guanylate cyclase (GTP pyophosphate-lyase, EC 4.6.1.2) activity from soluble and particulate fractions of ... | [
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PMID:15621 | [Activities of pulmonary phospholipases of fetal rats. Variations during development]. | Turnover of adult rat lung phospholipids implies intervention of phospholipases. This work clearly demonstrates: There is in fetal or adult rat lung an inactive form of phospholipase that is convertible to an active form by the action of lysed platelets. An increase of both active and inactive forms of the fetal enzyme... | [Activities of pulmonary phospholipases of fetal rats. Variations during development]. Turnover of adult rat lung phospholipids implies intervention of phospholipases. This work clearly demonstrates: There is in fetal or adult rat lung an inactive form of phospholipase that is convertible to an active form by the actio... | [
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PMID:15622 | Fatty acid synthetase complex from the insect Ceratitis capitata. | Fatty acid synthesis capacity of the insect Ceratitis capitata has been investigated in vitro from [1-14C]acetyl-CoA using homogenates at different stages of development. A maximum activity was observed after 5--6 days of larval development. But homogenates of the pharate adult insect did not show synthetic capacity of... | Fatty acid synthetase complex from the insect Ceratitis capitata. Fatty acid synthesis capacity of the insect Ceratitis capitata has been investigated in vitro from [1-14C]acetyl-CoA using homogenates at different stages of development. A maximum activity was observed after 5--6 days of larval development. But homogena... | [
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PMID:15623 | A proton magnetic relaxation study of ferric myoglobin and haemoglobin in water/ethanediol solutions. | Structural alterations of the haem vicinity of the high-spin derivatives of bovine ferric myoglobin (metmyoglobin) and human haemoglobin and the changes of the interaction with inositol hexaphosphate induced by ethanediol were monitored by solvent-proton magnetic relaxation. On addition of ethanediol up to 60% the fluo... | A proton magnetic relaxation study of ferric myoglobin and haemoglobin in water/ethanediol solutions. Structural alterations of the haem vicinity of the high-spin derivatives of bovine ferric myoglobin (metmyoglobin) and human haemoglobin and the changes of the interaction with inositol hexaphosphate induced by ethaned... | [
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PMID:15624 | Structural studies by proton magnetic relaxation of stereochemical probes. II. Allosteric effects in human methaemoglobin. | The haem-iron accessibility to solvent molecules in human aquomet- and fluoromethaemoglobin was studied by the magnetic relaxation of protons from a stereochemical probe (methanol in deuterated solutions) in its dependence on allosteric effects induced by inositol hexaphosphate and pH between 5.5 and 8.5. The exchange ... | Structural studies by proton magnetic relaxation of stereochemical probes. II. Allosteric effects in human methaemoglobin. The haem-iron accessibility to solvent molecules in human aquomet- and fluoromethaemoglobin was studied by the magnetic relaxation of protons from a stereochemical probe (methanol in deuterated sol... | [
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PMID:15625 | Extracellular agglutination factor of myxamoebae produced by Dictyostelium discoideum NC-4. | A non-dyalyzable specific agglutination factor of myxamoebae obtained from culture broth during the growth phase of Dictyostelium discoideum NC-4 was thermostable but the agglutination activity disappeared below pH 5.0. In the case of formalinized myxamoebae, digestion of the factor with Pronase decreased the activity,... | Extracellular agglutination factor of myxamoebae produced by Dictyostelium discoideum NC-4. A non-dyalyzable specific agglutination factor of myxamoebae obtained from culture broth during the growth phase of Dictyostelium discoideum NC-4 was thermostable but the agglutination activity disappeared below pH 5.0. In the c... | [
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PMID:15626 | Modulation of protein phosphorylation by a factor purified from adipocytes. | 1. A factor which modulates the activity of cyclic AMP-dependent protein kinase copurifies from rat adipocytes with an inhibitor of adenylate cyclase. Purification and stability studies suggest that both effects reside in a single factor previously referred to as a feedback regulator. 2. The magnitude and direction of ... | Modulation of protein phosphorylation by a factor purified from adipocytes. 1. A factor which modulates the activity of cyclic AMP-dependent protein kinase copurifies from rat adipocytes with an inhibitor of adenylate cyclase. Purification and stability studies suggest that both effects reside in a single factor previo... | [
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PMID:15627 | Selective reactions of nucleobases under biological conditions. | Pentacyanonitrosylferrate/II/ complex reacts under biological conditions (pH= 7.5, T= 25-40 degrees C, dilute solution) selectively with nucleobases. The reaction with adenine and guanine probably leads to nitrosation. A new compound formed in the reaction with adenine is prepared; both this compound and the pentacyano... | Selective reactions of nucleobases under biological conditions. Pentacyanonitrosylferrate/II/ complex reacts under biological conditions (pH= 7.5, T= 25-40 degrees C, dilute solution) selectively with nucleobases. The reaction with adenine and guanine probably leads to nitrosation. A new compound formed in the reaction... | [
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PMID:15628 | Oxidation of procarbazine in the presence of Ti(IV). | The air oxidation of procarbazine in the presence of Ti(IV) was examined as a model system for the effects titanium has on oxidative processes and intermediates involving molecular oxygen. It was found that Ti(IV) inhibited oxidation when the substrate, procarbazine, was coordinated to titanium. This inhibition was mos... | Oxidation of procarbazine in the presence of Ti(IV). The air oxidation of procarbazine in the presence of Ti(IV) was examined as a model system for the effects titanium has on oxidative processes and intermediates involving molecular oxygen. It was found that Ti(IV) inhibited oxidation when the substrate, procarbazine,... | [
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PMID:15629 | Tungsten vs. Molybdenum in models for biological systems. | Biological systems show a marked preference for molybdenum over tungsten. Studies with methyliminodiacetic acid and L-cysteine have shown that the formation constants of the complexes with Mo(Vi) and W(VI) are very similar. These results imply that these elements would be bound with roughly equal strengths to an apoenz... | Tungsten vs. Molybdenum in models for biological systems. Biological systems show a marked preference for molybdenum over tungsten. Studies with methyliminodiacetic acid and L-cysteine have shown that the formation constants of the complexes with Mo(Vi) and W(VI) are very similar. These results imply that these element... | [
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PMID:15631 | [Characterization and separation of exocellular gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase and LD-carboxypeptidase from Bacillus sphaericus 9602]. | Two exocellular enzymes have been characterized in the culture media of sporulating Bacillus sphaericus 9602 : a gamma-D-glutamyl-(L) meso-diaminopimelate endopeptidase and a L-lysyl-D-alanine carboxypeptidase. These two enzymes and the corresponding membrane-bound peptidases found in Bacillus sphaericus and Bacillus s... | [Characterization and separation of exocellular gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase and LD-carboxypeptidase from Bacillus sphaericus 9602]. Two exocellular enzymes have been characterized in the culture media of sporulating Bacillus sphaericus 9602 : a gamma-D-glutamyl-(L) meso-diaminopimelate endope... | [
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0.0... |
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