id stringlengths 7 10 | title stringlengths 8 354 | content stringlengths 91 4.1k | contents stringlengths 164 4.24k | embedding listlengths 384 384 |
|---|---|---|---|---|
PMID:16868 | Regulation of enzyme synthesis by the glutamine synthetase of Salmonella typhimurium: a factor in addition to glutamine synthetase is required for activation of enzyme formation. | In Klebsiella aerogenes but not in Salmonella typhimurium glutamine synthetase can function during nitrogen-limited growth to increase the rate of synthesis of histidase from the hut genes of S. typhimurium 15-59 (hutS. 15-59). Formation of proline oxidase is also not increased in nitrogen-limited cultures of S. typhim... | Regulation of enzyme synthesis by the glutamine synthetase of Salmonella typhimurium: a factor in addition to glutamine synthetase is required for activation of enzyme formation. In Klebsiella aerogenes but not in Salmonella typhimurium glutamine synthetase can function during nitrogen-limited growth to increase the ra... | [
-0.010714641772210598,
-0.026798846200108528,
-0.03593479096889496,
-0.012889113277196884,
0.061199646443128586,
0.0050073545426130295,
0.04931296780705452,
0.040709786117076874,
-0.07578327506780624,
0.02220989018678665,
-0.01087883673608303,
-0.06427756696939468,
0.033506542444229126,
-0... |
PMID:16870 | Alkaline serine proteinases D and E of Streptomyces griseus K-1. | Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide disc gel electrophoresis. The molecular weights of these enzymes we... | Alkaline serine proteinases D and E of Streptomyces griseus K-1. Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide di... | [
-0.09992102533578873,
-0.0077041145414114,
-0.05862684175372124,
0.008048111572861671,
0.045240674167871475,
-0.059311043471097946,
0.017651766538619995,
0.04001893848180771,
-0.027608046308159828,
-0.011429288424551487,
-0.00482726888731122,
-0.07385527342557907,
-0.008392758667469025,
0.... |
PMID:16871 | Amino acid sequence of a cyanogen bromide fragment containing the two tryptophanyl residues of lobster arginine kinase (Homarus vulgaris). | Lobster arginine kinase [EC 2.7.3.3] contains 2 tryptophanyl residues and 9 methionyl residues. The whole carboxymethylated protein was first subjected to CNBr cleavage and the resulting fragments were isolated by gel filtration and other experimental approaches. One fragment, CB5, which contains 60 residues including ... | Amino acid sequence of a cyanogen bromide fragment containing the two tryptophanyl residues of lobster arginine kinase (Homarus vulgaris). Lobster arginine kinase [EC 2.7.3.3] contains 2 tryptophanyl residues and 9 methionyl residues. The whole carboxymethylated protein was first subjected to CNBr cleavage and the resu... | [
-0.07064060866832733,
-0.04491262137889862,
-0.057670459151268005,
-0.06949492543935776,
-0.015213933773338795,
0.015770724043250084,
0.020603734999895096,
0.0058843097649514675,
-0.04441513121128082,
0.020524289458990097,
0.042017776519060135,
-0.09106644243001938,
0.008126896806061268,
0... |
PMID:16872 | Purification and characterization of arylamidase from monkey brain. | Arylamidase [EC3.4.11.2] was isolated from monkey brain extract and purified about 2100-fold in approximately 11% yield by a six-step procedure comprising extraction from monkey brain homogenate, ammonium sulfate fractionation, first hydroxylapatite chromatography, DEAE-cellulose chromatography, Sephadex G-200 gell fil... | Purification and characterization of arylamidase from monkey brain. Arylamidase [EC3.4.11.2] was isolated from monkey brain extract and purified about 2100-fold in approximately 11% yield by a six-step procedure comprising extraction from monkey brain homogenate, ammonium sulfate fractionation, first hydroxylapatite ch... | [
-0.09866109490394592,
-0.029951894655823708,
-0.08903659880161285,
0.0032965911086648703,
0.04180840402841568,
-0.0334617905318737,
0.02977452613413334,
0.06579195708036423,
-0.03671029582619667,
0.022718630731105804,
0.029995614662766457,
-0.04654712602496147,
-0.010771997272968292,
0.005... |
PMID:16873 | Anhydrotrypsin: new features in ligand interactions revealed by affinity chromatography and thionine replacement. | Anhydrotrypsin was isolated in high purity from the product of base elimination from phenylmethanesulfonyl-trypsin, by a single operation of affinity chromatography. The adsorbent used for the chromatography was an agarose derivative coupled with peptides containing C-terminal arginine residues. As the affinity of the ... | Anhydrotrypsin: new features in ligand interactions revealed by affinity chromatography and thionine replacement. Anhydrotrypsin was isolated in high purity from the product of base elimination from phenylmethanesulfonyl-trypsin, by a single operation of affinity chromatography. The adsorbent used for the chromatograph... | [
-0.11957589536905289,
-0.014182373881340027,
-0.07852810621261597,
0.01313021406531334,
0.02533595822751522,
-0.05352816358208656,
0.08023989200592041,
0.007490796968340874,
-0.0732882171869278,
-0.0021877035032957792,
0.0335112139582634,
-0.0746203064918518,
-0.015062506310641766,
0.08514... |
PMID:16874 | Glutathione reductase in the sea urchin egg. III. Activation of the complex form by proteinases. | The G-200 flow-through fraction of the extract of sea urchin eggs contained a complex form of glutathione reductase (GR) [EC 1.6.4.2]. The complex was unstable and gradually dissociated with ain increase in GR activity. The activation was facilitated by high concentrations of EDTA, KCI or (NH4)2SO4. The rate of activat... | Glutathione reductase in the sea urchin egg. III. Activation of the complex form by proteinases. The G-200 flow-through fraction of the extract of sea urchin eggs contained a complex form of glutathione reductase (GR) [EC 1.6.4.2]. The complex was unstable and gradually dissociated with ain increase in GR activity. The... | [
-0.05927588790655136,
-0.056719135493040085,
-0.07362731546163559,
0.04280684143304825,
0.023632338270545006,
-0.019640080630779266,
0.050610676407814026,
0.05819940194487572,
-0.057121217250823975,
-0.043448686599731445,
-0.018265191465616226,
-0.0736381858587265,
-0.008603856898844242,
0... |
PMID:16875 | A new enzyme, NADPH-dihydropteridine reductase in bovine liver. | An enzyme designated as NADPH-dihydropteridine reductase was found in the extract of bovine liver and partially purified. In contrast to NADH-dpendent dihydropteridine reductase [EC 1.6.99.7], the enzyme catalyzes the reduction of quinonid-dihydropterin to tetrahydropterin in the presence of NADPH. The two enzymes were... | A new enzyme, NADPH-dihydropteridine reductase in bovine liver. An enzyme designated as NADPH-dihydropteridine reductase was found in the extract of bovine liver and partially purified. In contrast to NADH-dpendent dihydropteridine reductase [EC 1.6.99.7], the enzyme catalyzes the reduction of quinonid-dihydropterin to... | [
-0.05987785384058952,
0.0007058451301418245,
-0.049671635031700134,
0.058654461055994034,
0.007585412822663784,
-0.046077974140644073,
0.05103364959359169,
0.08819438517093658,
-0.00421122508123517,
-0.04370701313018799,
0.021113533526659012,
-0.04011182487010956,
-0.014361603185534477,
-0... |
PMID:16876 | Glycolysis of red cells suspended in solutions of impermeable solutes. Intracellular pH and glycolysis. | The glycolytic rate human red cells suspended in a sucrose medium of low or physiological pH was higher than that of the cells suspended in Ringer's medium of the same. pH. The medium pHP-glycolytic rate curve of red cells suspended in soucrose media shifted to the acidic side by about one unit compared with that of ce... | Glycolysis of red cells suspended in solutions of impermeable solutes. Intracellular pH and glycolysis. The glycolytic rate human red cells suspended in a sucrose medium of low or physiological pH was higher than that of the cells suspended in Ringer's medium of the same. pH. The medium pHP-glycolytic rate curve of red... | [
-0.05117713287472725,
-0.021985089406371117,
-0.009976617060601711,
0.03957860916852951,
0.04242745414376259,
-0.026092123240232468,
0.044890012592077255,
0.01502743735909462,
-0.0069260611198842525,
0.0012680866057053208,
0.018170194700360298,
-0.10813097655773163,
-0.024901902303099632,
... |
PMID:16877 | Actin-induced local conformational change in the myosin molecule. I. Effect of metal ions and nucleotides on the conformational change around a specific thiol group (S2) of heavy meromyosin. | As previously reported when a specific thiol group, S2, of myosin reacts with N-ethylmaleimide (NEM), its Ca2+-ATPase activity is decreased. Therefore, the reactivity of S2 can be estimated by measuring the decrement of the enzymatic activity. Using the change in the reactivity as a structural probe, we investigated wh... | Actin-induced local conformational change in the myosin molecule. I. Effect of metal ions and nucleotides on the conformational change around a specific thiol group (S2) of heavy meromyosin. As previously reported when a specific thiol group, S2, of myosin reacts with N-ethylmaleimide (NEM), its Ca2+-ATPase activity is... | [
-0.09596051275730133,
-0.04670064151287079,
0.005173024255782366,
0.02227765880525112,
0.027010714635252953,
-0.014645208604633808,
0.06685560941696167,
0.02791525609791279,
-0.051442839205265045,
-0.030063698068261147,
0.009564705193042755,
-0.038946349173784256,
-0.06451477110385895,
0.0... |
PMID:16878 | Ciliary dynein from sea urchin embryos. | Axonemal dynein ATPase [EC 3.6.1.3] was extracted from cilia of sea urchin embryos for a study of its enzymatic properties. Sedimentation analysis on a sucrose density gradient revealed that ATPase activity was associated with the 12S particles. The partially purified 12S enzyme was characterized mainly with regard to ... | Ciliary dynein from sea urchin embryos. Axonemal dynein ATPase [EC 3.6.1.3] was extracted from cilia of sea urchin embryos for a study of its enzymatic properties. Sedimentation analysis on a sucrose density gradient revealed that ATPase activity was associated with the 12S particles. The partially purified 12S enzyme ... | [
-0.09457546472549438,
-0.07823222130537033,
-0.016598964110016823,
0.004487459547817707,
0.020769046619534492,
-0.022731153294444084,
0.028904356062412262,
0.01576077938079834,
0.007442671339958906,
-0.05740584433078766,
0.008596203289926052,
-0.07121691852807999,
-0.02448285184800625,
0.0... |
PMID:16879 | Amino acid sequences around 1, 2-epoxy-3-(p-nitrophenoxy)propane-reactive residues in rhizopus chinensis acid protease: homology with pepsin and rennin. | Two different peptides containing an aspartyl residue reactive with 1, 2-epoxy-3-(p-nitrophenoxy)propane (EPNP) in the acid protease from Rhizopus chinensis were isolated from a peptic digest of the EPNP-modified enzyme. One of the peptides was sequenced as Asp-Thr-Gly-Ser-Asp. The amino acid sequence had very high hom... | Amino acid sequences around 1, 2-epoxy-3-(p-nitrophenoxy)propane-reactive residues in rhizopus chinensis acid protease: homology with pepsin and rennin. Two different peptides containing an aspartyl residue reactive with 1, 2-epoxy-3-(p-nitrophenoxy)propane (EPNP) in the acid protease from Rhizopus chinensis were isola... | [
-0.09929952025413513,
0.005684985313564539,
-0.057615380734205246,
0.017374197021126747,
0.011952092871069908,
-0.0037175517063587904,
0.041607506573200226,
0.016611743718385696,
-0.01976841129362583,
0.03809705376625061,
0.016358522698283195,
-0.05685300752520561,
0.04058392718434334,
0.0... |
PMID:16880 | Phosphoenolypyruvate synthetase of Escherichia coli: molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate. | Phosphoenolypyruvate synthetase of Escherichia coli has been shown to be a dimer of molecular weight 150,000. The constituent subunits appear to be identical. The enzyme tends to dissociate to monomers at low protein concentration, but the tendency is much diminished in the phosphoenzyme form, suggesting that enzyme ph... | Phosphoenolypyruvate synthetase of Escherichia coli: molecular weight, subunit composition, and identification of phosphohistidine in phosphoenzyme intermediate. Phosphoenolypyruvate synthetase of Escherichia coli has been shown to be a dimer of molecular weight 150,000. The constituent subunits appear to be identical.... | [
-0.11376883089542389,
-0.062706358730793,
-0.031953584402799606,
-0.015203338116407394,
0.0011273159179836512,
-0.06132172793149948,
0.00037569805863313377,
-0.00892502162605524,
-0.0028058141469955444,
-0.021105745807290077,
0.07049500197172165,
-0.0679696649312973,
-0.02565842866897583,
... |
PMID:16881 | Source of residual Bohr effect in hemoglobin oxidation. | The hemoglobin oxidation Bohr effect is larger than the ligation Bohr effect, even when the former is corrected for any ionization of the water molecule bound to the ferric iron of methemoglobin. This residual oxidation Bohr effect is here shown to be solely caused by the influence of the positively charged ferriheme, ... | Source of residual Bohr effect in hemoglobin oxidation. The hemoglobin oxidation Bohr effect is larger than the ligation Bohr effect, even when the former is corrected for any ionization of the water molecule bound to the ferric iron of methemoglobin. This residual oxidation Bohr effect is here shown to be solely cause... | [
-0.03138250112533569,
0.0973399430513382,
-0.06705804169178009,
0.022979432716965675,
0.03561927005648613,
-0.08246589452028275,
0.029829302802681923,
0.013809881173074245,
-0.057078514248132706,
-0.0484224371612072,
0.02977626770734787,
-0.07340829074382782,
-0.012440433725714684,
0.04044... |
PMID:16883 | Magnetic resonance studies of concanavalin A: assignment of histidine resonances in 220 MHz proton spectrum of complexes with Co2+ and Zn2+. | The low field regions of the 220 MHz proton magnetic resonance spectra of concanavalin A (Con A) complexes with metal ions show well resolved resonances from the C2 protons of histidine side chains. Shifts of these resonances are observed when Zn2+ ions at the transition metal ion binding site, S1, are replaced by CO2 ... | Magnetic resonance studies of concanavalin A: assignment of histidine resonances in 220 MHz proton spectrum of complexes with Co2+ and Zn2+. The low field regions of the 220 MHz proton magnetic resonance spectra of concanavalin A (Con A) complexes with metal ions show well resolved resonances from the C2 protons of his... | [
-0.12644784152507782,
0.02149347774684429,
-0.056535519659519196,
0.006505194120109081,
-0.014125530607998371,
-0.010932737961411476,
0.03887241333723068,
0.008197878487408161,
-0.021634159609675407,
-0.009868594817817211,
0.04568382725119591,
-0.08967607468366623,
0.0061314841732382774,
-... |
PMID:16885 | Bovine adrenal tyrosine hydroxylase: purification and properties. | Bovine adrenal tyrosine hydroxylase has been obtained in a form that is 85 to 90% pure. Sodium dodecyl sulfate-gel electrophoresis and density gradient centrifugation studies have established that the subunit molecular weight of the chymotrypsin-solubilized enzyme is 34,000. The presence of iron in the purified enzyme ... | Bovine adrenal tyrosine hydroxylase: purification and properties. Bovine adrenal tyrosine hydroxylase has been obtained in a form that is 85 to 90% pure. Sodium dodecyl sulfate-gel electrophoresis and density gradient centrifugation studies have established that the subunit molecular weight of the chymotrypsin-solubili... | [
-0.06149918586015701,
-0.0029041245579719543,
-0.13517123460769653,
0.03365248441696167,
-0.05811738222837448,
-0.0505402497947216,
0.07288269698619843,
0.00915568508207798,
-0.03466515243053436,
-0.025298573076725006,
0.009138398803770542,
-0.09424765408039093,
-0.033024150878190994,
0.01... |
PMID:16888 | Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis. | We have isolated glutamic acid 5-methyl ester from an Escherichia coli protein that is involved in chemotaxis. The bacteria were first incubated with [methyl-3H]methionine under conditions which are known to result in methylation of the protein. The protein, isolated by gel electrophoresis, was then digested by success... | Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis. We have isolated glutamic acid 5-methyl ester from an Escherichia coli protein that is involved in chemotaxis. The bacteria were first incubated with [methyl-3H]methionine under conditions which are known to result... | [
-0.06290753185749054,
-0.011618806049227715,
-0.04406384378671646,
0.006297538988292217,
0.03665631636977196,
-0.029380302876234055,
0.04027927294373512,
0.03763825446367264,
-0.011964358389377594,
-0.014235962182283401,
0.0391254685819149,
-0.05987821891903877,
0.009291657246649265,
-0.02... |
PMID:16889 | Induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in HeLa cells by glucocorticoids. | The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) has been demonstrated both in homogenates and microsomes of the S3G strain of HeLa cells. It was increased 8- to 10-fold by the removal of serum from the growth medium. The presence of steroids, specifically of the glucocor... | Induction of 3-hydroxy-3-methylglutaryl coenzyme A reductase in HeLa cells by glucocorticoids. The activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase, EC 1.1.1.34) has been demonstrated both in homogenates and microsomes of the S3G strain of HeLa cells. It was increased 8- to 10-fold by the ... | [
-0.009640999138355255,
0.014773745089769363,
-0.04482351243495941,
0.05754663795232773,
0.025430377572774887,
-0.0029111250769346952,
0.010734312236309052,
0.004770439583808184,
-0.018528588116168976,
-0.02908499725162983,
-0.011324121616780758,
-0.0889023095369339,
0.01045664306730032,
-0... |
PMID:16891 | Purification and properties of a plant endonuclease specific for apurinic sites. | An endonuclease which hydrolyzes depurinated DNA has been isolated from Phaseolus multiflorus enbryos; it has a molecular weight around 40,000. The enzyme is specific for apurinic sites; it has no action on normal DNA strands or on alkylated sites, and is without exonulcease activity. The rate of phosphoester bond hydr... | Purification and properties of a plant endonuclease specific for apurinic sites. An endonuclease which hydrolyzes depurinated DNA has been isolated from Phaseolus multiflorus enbryos; it has a molecular weight around 40,000. The enzyme is specific for apurinic sites; it has no action on normal DNA strands or on alkylat... | [
-0.037491098046302795,
-0.01480111014097929,
0.007165813352912664,
0.0016375211998820305,
-0.006116406060755253,
-0.09030307829380035,
0.013206839561462402,
0.06351697444915771,
-0.03952629491686821,
-0.0028243516571819782,
0.019203685224056244,
-0.06850794702768326,
-0.012987589463591576,
... |
PMID:16892 | Estrogen-binding protein from rat preputial gland: purification and characterization. | Cytosol from the rat preputial gland has been shown to contain a protein which binds both estrone and estradiol. The protein, after a 26-fold purification from the cytosol of female Sprague-Dawley rats, migrated as one band during electrophoresis in sodium dodecyl sulfate on acrylamide gel. The electrophoretic mobility... | Estrogen-binding protein from rat preputial gland: purification and characterization. Cytosol from the rat preputial gland has been shown to contain a protein which binds both estrone and estradiol. The protein, after a 26-fold purification from the cytosol of female Sprague-Dawley rats, migrated as one band during ele... | [
-0.03311240300536156,
-0.030805056914687157,
-0.0003868832136504352,
-0.03173705190420151,
0.05544687807559967,
-0.06329555809497833,
0.09907356649637222,
0.003745387075468898,
0.010084852576255798,
-0.013857186771929264,
-0.03406889736652374,
-0.029672468081116676,
0.0010645275469869375,
... |
PMID:16894 | Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii. | Chitin synthetase was isolated and purified 120-fold from the supernatant fraction (54,500 X g) of broken yeast cells of Mucor rouxii. The purified preparations consisted mainly of chitin synthetase particles (chitosomes) with an average size larger than 7 X 10(6) daltons (by gel filtration) and an average sedimentatio... | Properties of chitin synthetase in isolated chitosomes from yeast cells of Mucor rouxii. Chitin synthetase was isolated and purified 120-fold from the supernatant fraction (54,500 X g) of broken yeast cells of Mucor rouxii. The purified preparations consisted mainly of chitin synthetase particles (chitosomes) with an a... | [
-0.06530894339084625,
-0.05132870748639107,
0.0033161891624331474,
0.0073942276649177074,
0.021862810477614403,
-0.011932986788451672,
0.03209506720304489,
0.017372485250234604,
-0.05284924805164337,
0.05594433844089508,
0.002243976341560483,
-0.0715465173125267,
-0.02905006892979145,
-0.0... |
PMID:16896 | DNA polymerase of mitochondria is a gamma-polymerase. | Mitochondria isolated from rat liver cells or mycoplasma-free HeLa cells contain a single DNA polymerase activity which is closely related to, or identical to, the DNA polymerase gamma activity found in the homologous cell. In rat liver cells, about 16% of the total cytoplasmic gamma-polymerase activity is found associ... | DNA polymerase of mitochondria is a gamma-polymerase. Mitochondria isolated from rat liver cells or mycoplasma-free HeLa cells contain a single DNA polymerase activity which is closely related to, or identical to, the DNA polymerase gamma activity found in the homologous cell. In rat liver cells, about 16% of the total... | [
-0.008895063772797585,
-0.003906467463821173,
-0.014686746522784233,
0.005000509787350893,
0.030362598598003387,
0.004680130630731583,
0.03678242489695549,
-0.002703330712392926,
0.02671094797551632,
-0.03385956585407257,
0.056961607187986374,
-0.018206626176834106,
-0.08415554463863373,
0... |
PMID:16897 | Characterization of plasma membrane adenosine triphosphatase of Neurospora crassa. | It has been proposed (Slayman, C.L., Long W.S., and Lu, C.Y.-H. (1973) J. Membr. Biol. 14, 305--338) that in Neurospora crassa, a plasma membrane ATPase functions to pump H+ ions out of the cell, thereby generating an electrochemical gradient that can drive transport processes. Using the concanavalin A method of Scarbo... | Characterization of plasma membrane adenosine triphosphatase of Neurospora crassa. It has been proposed (Slayman, C.L., Long W.S., and Lu, C.Y.-H. (1973) J. Membr. Biol. 14, 305--338) that in Neurospora crassa, a plasma membrane ATPase functions to pump H+ ions out of the cell, thereby generating an electrochemical gra... | [
-0.0685000792145729,
-0.031080812215805054,
0.03120996616780758,
-0.015569807961583138,
-0.0009118824382312596,
-0.03025616705417633,
0.03847009316086769,
0.0370955616235733,
-0.030070539563894272,
0.005859143566340208,
0.03326774761080742,
-0.07476997375488281,
-0.053327493369579315,
-0.0... |
PMID:16898 | Identification of folate binding macromolecule in rabbit choroid plexus. | A macromolecular binder of folic acid and folic acid derivatives has been identified in the particulate fraction of homogenates of rabbit choroid plexus. Within the choroid plexus, there are 2.3 nmol of folate-binding activity (binder) per g of tissue. The molecular weight of the folate binder complex, separated from t... | Identification of folate binding macromolecule in rabbit choroid plexus. A macromolecular binder of folic acid and folic acid derivatives has been identified in the particulate fraction of homogenates of rabbit choroid plexus. Within the choroid plexus, there are 2.3 nmol of folate-binding activity (binder) per g of ti... | [
-0.003293731017038226,
-0.0272735096514225,
-0.029901938512921333,
-0.022655989974737167,
0.003967301454395056,
0.025422943755984306,
0.07639791071414948,
0.04671885073184967,
-0.015137001872062683,
0.03058394230902195,
0.023330921307206154,
-0.0979752391576767,
0.03865215182304382,
-0.040... |
PMID:16900 | Repressible alkaline phosphatase from Thermus aquaticus: associated phosphodiesterase activity. | A repressible alkaline phosphatase has been isolated from the extreme bacterial thermophile. Thermus aquaticus, and has been purified to homogeneity as judged by disc acrylamide electrophoresis and sodium dodecyl sulfate electrophoresis. Upon investigation, the purified enzyme was shown to hydrolyze certain phosphodies... | Repressible alkaline phosphatase from Thermus aquaticus: associated phosphodiesterase activity. A repressible alkaline phosphatase has been isolated from the extreme bacterial thermophile. Thermus aquaticus, and has been purified to homogeneity as judged by disc acrylamide electrophoresis and sodium dodecyl sulfate ele... | [
-0.030056564137339592,
0.014445368200540543,
0.0011699609458446503,
0.028288597241044044,
-0.013136129826307297,
-0.07676428556442261,
0.06474918872117996,
0.023322314023971558,
-0.07319819927215576,
-0.04324992746114731,
-0.006128102075308561,
-0.09865472465753555,
0.005573267117142677,
-... |
PMID:16904 | Specificity of 2-keto-3-deoxygluconate-6-P aldolase for open chain form of 2-keto-3-deoxygluconate-6-P. | 2-Keto-3-deoxygluconate-6-P exists as an euqilibrium of three forms at 25 degrees measurable by 13C NMR: alpha-furanose anomer (41%), beta-furanose anomer (50%), and open chain keto (9%). The three forms are interconverted rapidly (greater than 0.5 s-1) so that the unidirectional rates of furanose ring opening and clos... | Specificity of 2-keto-3-deoxygluconate-6-P aldolase for open chain form of 2-keto-3-deoxygluconate-6-P. 2-Keto-3-deoxygluconate-6-P exists as an euqilibrium of three forms at 25 degrees measurable by 13C NMR: alpha-furanose anomer (41%), beta-furanose anomer (50%), and open chain keto (9%). The three forms are intercon... | [
-0.09935808926820755,
-0.0538715161383152,
-0.029447156935930252,
0.038748133927583694,
0.0036507986951619387,
-0.013629918918013573,
-0.004205106757581234,
0.006188271101564169,
-0.069056436419487,
-0.04723324254155159,
0.042173758149147034,
-0.052603546530008316,
-0.038829926401376724,
0... |
PMID:16907 | Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase. | Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular routes with catalase modified by covalent attachment of PEG-1900 to 43% ... | Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase. Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were i... | [
-0.04390477389097214,
-0.026686228811740875,
-0.02204038016498089,
0.02750422991812229,
0.011438866145908833,
-0.06374574452638626,
0.05748791620135307,
0.048534076660871506,
-0.042736880481243134,
0.00340671488083899,
-0.0005166652845218778,
-0.05935110151767731,
-0.007071655709296465,
0.... |
PMID:16908 | Human thrombins. Production, evaluation, and properties of alpha-thrombin. | Human alpha-thrombin, the thromboplastin activation product of prothrombin with high clotting and esterase activity, was produced from Cohn Fraction III paste. The procedure started with 0.4 to 3.2 kg of frozen paste and was completed in 2 or 3 days. Some 23 g of thrombin were recorded for 65 quantitated preparations m... | Human thrombins. Production, evaluation, and properties of alpha-thrombin. Human alpha-thrombin, the thromboplastin activation product of prothrombin with high clotting and esterase activity, was produced from Cohn Fraction III paste. The procedure started with 0.4 to 3.2 kg of frozen paste and was completed in 2 or 3 ... | [
-0.0925036370754242,
0.03573725000023842,
-0.05556541308760643,
0.011114867404103279,
0.03715283423662186,
0.027970101684331894,
0.06473620235919952,
0.03293390944600105,
-0.06702372431755066,
0.035408638417720795,
-0.01812652312219143,
-0.0853804275393486,
-0.02808416448533535,
0.03274710... |
PMID:16910 | Interaction of apoA-II from human high density lipoprotein with lysolecithin. | The effects of lysolecithin and hexadecyltrimethylammonium bromide on the structure and stability of apoA-II from human high density lipoprotein have been evalued by circular dichroism and fluorescence measurements. There is a profound enhancement in the stability of apoA-II to guanidinium hydrochloride denaturation wh... | Interaction of apoA-II from human high density lipoprotein with lysolecithin. The effects of lysolecithin and hexadecyltrimethylammonium bromide on the structure and stability of apoA-II from human high density lipoprotein have been evalued by circular dichroism and fluorescence measurements. There is a profound enhanc... | [
-0.08298563957214355,
-0.023684585466980934,
-0.035630688071250916,
-0.009829278104007244,
-0.03244759514927864,
-0.04991069436073303,
0.041663989424705505,
0.028424348682165146,
-0.03908795863389969,
-0.03865549713373184,
-0.017289502546191216,
-0.07366039603948593,
0.03644859418272972,
0... |
PMID:16912 | Liver microsomal epoxide hydrase. | 1. The substrate specificity of membrane-bound and purified epoxide hydrase from rat liver microsomes has been studied. Both enzyme preparations catalyzed the hydration of a variety of alkene oxidase as well as arene oxides of several polycyclic aromatic hydrocarbons. 2. Unlike the membrane-bound enzyme, the rate of hy... | Liver microsomal epoxide hydrase. 1. The substrate specificity of membrane-bound and purified epoxide hydrase from rat liver microsomes has been studied. Both enzyme preparations catalyzed the hydration of a variety of alkene oxidase as well as arene oxides of several polycyclic aromatic hydrocarbons. 2. Unlike the mem... | [
-0.028628602623939514,
0.019465556368231773,
0.012789944186806679,
0.013355677947402,
0.003995287697762251,
-0.03769970312714577,
0.0051322802901268005,
0.030175285413861275,
-0.0281887985765934,
-0.0015738675137981772,
-0.021290889009833336,
-0.03842884674668312,
-0.053194016218185425,
-0... |
PMID:16913 | Acidic dissociation constants of folic acid, dihydrofolic acid, and methotrexate. | The acidic dissociation constants in the range HO--1.5 to pH 7 of folic acid, dihydrofolic acid, methopterin (N(10)methylfolic acid), and methotrexate have been measured by potentiometric and spectrophotometric titrations. Assignment of these dissociations was made by comparison to model compounds, by proton magnetic r... | Acidic dissociation constants of folic acid, dihydrofolic acid, and methotrexate. The acidic dissociation constants in the range HO--1.5 to pH 7 of folic acid, dihydrofolic acid, methopterin (N(10)methylfolic acid), and methotrexate have been measured by potentiometric and spectrophotometric titrations. Assignment of t... | [
-0.05156177654862404,
0.0018495724070817232,
-0.0285661444067955,
-0.00426472956314683,
0.022393127903342247,
-0.0008906954317353666,
0.030474700033664703,
0.04754176735877991,
-0.03510848432779312,
-0.025833670049905777,
0.0460096150636673,
-0.12112744152545929,
0.0381816104054451,
0.0381... |
PMID:16914 | Protein modification enzymes associated with the protein-synthesizing complex from rabbit reticulocytes. Protein kinase, phosphoprotein phosphatase, and acetyltransferase. | A number of protein modification activities are present in the protein-synthesizing complex isolated from rabbit reticulocytes. These enzymes are solubilized by sedimentation of the ribosomes through buffered sucrose containing 0.5 M KCl, and have been partially purified from the high salt wash fraction by chromatograp... | Protein modification enzymes associated with the protein-synthesizing complex from rabbit reticulocytes. Protein kinase, phosphoprotein phosphatase, and acetyltransferase. A number of protein modification activities are present in the protein-synthesizing complex isolated from rabbit reticulocytes. These enzymes are so... | [
-0.0800958201289177,
-0.05072503164410591,
-0.06446239352226257,
-0.0017787982942536473,
0.01213825587183237,
-0.0277666375041008,
0.052147895097732544,
0.0299523938447237,
-0.0645827054977417,
0.006685370113700628,
0.032544564455747604,
-0.10286732763051987,
0.032220881432294846,
-0.01217... |
PMID:16917 | Purification and properties of a membrane-associated, folate-binding protein from Lactobacillus casei. | A folate-binding protein has been solubilized from Lactobacillus casei by treatment of membrane preparations with Triton X-100 in the presence of [3H]folate. The protein-folate complex was purified 100-fold and recovered in a 22% yield by adsorption and elution from microgranular silica (Quso G-32), followed by passage... | Purification and properties of a membrane-associated, folate-binding protein from Lactobacillus casei. A folate-binding protein has been solubilized from Lactobacillus casei by treatment of membrane preparations with Triton X-100 in the presence of [3H]folate. The protein-folate complex was purified 100-fold and recove... | [
-0.06607507914304733,
-0.016340425238013268,
-0.06440133601427078,
0.002776237204670906,
0.012942015193402767,
-0.05391380190849304,
0.07332953065633774,
0.0701632872223854,
-0.0275439340621233,
0.013138782232999802,
-0.014992918819189072,
-0.13318276405334473,
0.021304314956068993,
-0.028... |
PMID:16919 | Purification and properties of NADPH-dependent aldehyde reductase from human liver. | An aldehyde reductase (EC 1.1.1.2) from human liver has been purified to homogeneity. The enzyme is NADPH-dependent, prefers aromatic to aliphatic aldehydes as substrates, and is inhibited by barbiturates and hydantoins. The following physicochemical parameters were determined: molecular weight, 36,200; sedimentation c... | Purification and properties of NADPH-dependent aldehyde reductase from human liver. An aldehyde reductase (EC 1.1.1.2) from human liver has been purified to homogeneity. The enzyme is NADPH-dependent, prefers aromatic to aliphatic aldehydes as substrates, and is inhibited by barbiturates and hydantoins. The following p... | [
-0.07513494789600372,
-0.011476847343146801,
-0.07474789023399353,
0.04884423315525055,
0.0274646133184433,
-0.020817896351218224,
0.07069157063961029,
0.009495498612523079,
-0.04342946037650108,
-0.03306492418050766,
0.002577273640781641,
-0.028639167547225952,
-0.010002057068049908,
-0.0... |
PMID:16920 | Species-specific aggregation factor in sponges. Sialyltransferase associated with aggregation factor. | The sialyltransferase (= glycoprotein-sialic acid transferase) was studied in the sponge Geodia cydonium, a mesozoan organism. The experiments were performed both in intact cellular and in isolated enzyme systems. It is shown, that desialylated cells show a lower aggregation potency than the controls. During aggregatio... | Species-specific aggregation factor in sponges. Sialyltransferase associated with aggregation factor. The sialyltransferase (= glycoprotein-sialic acid transferase) was studied in the sponge Geodia cydonium, a mesozoan organism. The experiments were performed both in intact cellular and in isolated enzyme systems. It i... | [
-0.027270875871181488,
-0.05234239250421524,
-0.0223534032702446,
-0.006134025752544403,
-0.00934999343007803,
-0.08867882192134857,
0.06783854216337204,
0.04773488640785217,
-0.04579133167862892,
-0.025925306603312492,
0.09820804744958878,
-0.13443692028522491,
0.028843127191066742,
-0.03... |
PMID:16921 | Compartmentalization of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in heart tissue. | In rabbit heart homogenates about 50% of the cAMP-dependent protein kinase activity was associated with the low speed particulate fraction. In homogenates of rat or beef heart this fraction represented approximately 30% of the activity. The percentage of the enzyme in the particulate fraction was not appreciably affect... | Compartmentalization of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in heart tissue. In rabbit heart homogenates about 50% of the cAMP-dependent protein kinase activity was associated with the low speed particulate fraction. In homogenates of rat or beef heart this fraction ... | [
-0.01876799762248993,
-0.04706455394625664,
-0.023596901446580887,
-0.039766378700733185,
0.0005756265600211918,
-0.008635337464511395,
0.057648658752441406,
-0.005311418324708939,
-0.045972708612680435,
0.009808468632400036,
-0.003741212422028184,
-0.10986948013305664,
0.0034289334435015917... |
PMID:16922 | Interaction of glucocorticoid hormones and cyclic nucleotides in induction of tyrosine aminotransferase in cultured hepatoma cells. | Reproducible induction of the enzyme tyrosine aminotransferase by dibutyryl cAMP (Bt2cAMP) in a line of HTC hepatoma cells in suspension culture requires that the cells be preinduced with dexamethasone, a synthetic glucocorticoid which itself induces tyrosine aminotransferase. Concentrations of dexamethasone that do no... | Interaction of glucocorticoid hormones and cyclic nucleotides in induction of tyrosine aminotransferase in cultured hepatoma cells. Reproducible induction of the enzyme tyrosine aminotransferase by dibutyryl cAMP (Bt2cAMP) in a line of HTC hepatoma cells in suspension culture requires that the cells be preinduced with ... | [
-0.005325306672602892,
0.001604712102562189,
-0.03239559754729271,
0.011876750737428665,
0.01750134862959385,
0.009396126493811607,
0.04449012875556946,
0.00602334039285779,
-0.05141163617372513,
-0.058938972651958466,
0.024135036394000053,
-0.054592858999967575,
0.04712912440299988,
0.027... |
PMID:16924 | Effect of hydrostatic pressure on ligand binding to hemoglobin. | Increase in hydrostatic pressure to 1000 atm increased the affinity of human and menhaden (Brevoortia tyrannus) hemoglobins for oxygen. With necessary assumptions about the form of the equilibrium curve, and after correction for changes in pH and volume due to pressure, the increase in affinity is about 2-fold for both... | Effect of hydrostatic pressure on ligand binding to hemoglobin. Increase in hydrostatic pressure to 1000 atm increased the affinity of human and menhaden (Brevoortia tyrannus) hemoglobins for oxygen. With necessary assumptions about the form of the equilibrium curve, and after correction for changes in pH and volume du... | [
-0.08974168449640274,
0.0553458072245121,
-0.029241368174552917,
0.02970430627465248,
0.0001977854990400374,
-0.03673962876200676,
0.10825701802968979,
0.014341121539473534,
-0.07057157903909683,
-0.007382180541753769,
0.04421234875917435,
-0.08053021878004074,
-0.0068564945831894875,
-0.0... |
PMID:16925 | Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. | Methods for measurements of catabolic reduction charge (defined as NADH/(NADH+NAD+)) and anabolic reduction charge (defined as NADPH/(NADPH + NADP+)) are described using [14C]nicotinamide labeling of Escherichia coli cultures. Together with these parameters the adenylate energy charge (ATP + 1/2ADP)/(ATP + ADP + AMP) w... | Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. Methods for measurements of catabolic reduction charge (defined as NADH/(NADH+NAD+)) and anabolic reduction charge (defined as NADPH/(NADPH + NADP+)) are described using [14C]nicotinam... | [
-0.08130135387182236,
-0.0013587265275418758,
-0.03840756043791771,
0.01793932542204857,
0.04013456031680107,
0.0027033870574086905,
0.039725564420223236,
0.025074291974306107,
-0.04062087461352348,
-0.02264895662665367,
0.04939546063542366,
-0.06725367903709412,
0.01438870932906866,
0.058... |
PMID:16933 | Phosphoprotein phosphatase activity associated with the cytoplasmic membrane of Salmonella typhimurium and Escherichia coli. | Membrane-associated phosphoprotein phosphatase activity was demonstrated in extracts of Salmonella typhimurium and Escherichia coli. The active protein could be extracted from the membrane as a large water-soluble complex (Mr greater than 150,000). Maximal activity was observed at pH 6 to 7 in the presence of a divalen... | Phosphoprotein phosphatase activity associated with the cytoplasmic membrane of Salmonella typhimurium and Escherichia coli. Membrane-associated phosphoprotein phosphatase activity was demonstrated in extracts of Salmonella typhimurium and Escherichia coli. The active protein could be extracted from the membrane as a l... | [
-0.07951177656650543,
-0.018243588507175446,
-0.06209450215101242,
-0.010412761010229588,
0.01824312098324299,
-0.09612478315830231,
0.0985245555639267,
0.0745847076177597,
0.010150199756026268,
-0.014128328301012516,
0.02214393950998783,
-0.10150542855262756,
0.005360129754990339,
-0.0183... |
PMID:16935 | In vitro activation of the in vivo colony-forming units of the mouse yolk sac. | Experiments were performed to investigate the presence of colony-forming units (CFU) in the mouse embryonic yolk sac during the developmental period in which the yolk sac is the sole hemopoietic organ. Injection of yolk sac cell suspensions from normal embryos into syngeneic, lethally irradiated adult recipients evoked... | In vitro activation of the in vivo colony-forming units of the mouse yolk sac. Experiments were performed to investigate the presence of colony-forming units (CFU) in the mouse embryonic yolk sac during the developmental period in which the yolk sac is the sole hemopoietic organ. Injection of yolk sac cell suspensions ... | [
-0.02312811277806759,
-0.021344520151615143,
0.013766135089099407,
-0.006487409118562937,
-0.016107868403196335,
0.049834057688713074,
0.035668376833200455,
0.09958495199680328,
-0.00634388905018568,
-0.025593597441911697,
0.020676827058196068,
-0.08667819947004318,
-0.016593772917985916,
... |
PMID:16936 | The diverse effects of 5'-bromodeoxyuridine on enzyme activities in cultured H35 hepatoma cells. | Reuber (H35) hepatoma cells were grown in medium containing 10(-5)M bromodeoxyuridine (BrdU), which was incorporated into their DNA. Cell growth rate was unaffected by BrdU for the first two generations, after which it was reduced by about 50%. The effect of BrdU incorporation on the activities of several enzymes with ... | The diverse effects of 5'-bromodeoxyuridine on enzyme activities in cultured H35 hepatoma cells. Reuber (H35) hepatoma cells were grown in medium containing 10(-5)M bromodeoxyuridine (BrdU), which was incorporated into their DNA. Cell growth rate was unaffected by BrdU for the first two generations, after which it was ... | [
-0.008571387268602848,
-0.018377602100372314,
-0.021282123401761055,
0.01152164675295353,
-0.0227759201079607,
0.032229214906692505,
0.022444915026426315,
0.037092167884111404,
-0.06155218929052353,
-0.03948233276605606,
0.041681621223688126,
-0.038086049258708954,
0.01819017343223095,
0.0... |
PMID:16937 | The relation of cycling of intracellular pH to mitosis in the acellular slime mould Physarum polycephalum. | The relation between intracellular pH and the mitotic cycle of Physarum polycephalum was studied by two-independent techniques. Both techniques revealed a long term cycling of intracellular pH which has the same period as the mitotic cycle, Qualitative detection of the changes in intracellular pH was made by measuring ... | The relation of cycling of intracellular pH to mitosis in the acellular slime mould Physarum polycephalum. The relation between intracellular pH and the mitotic cycle of Physarum polycephalum was studied by two-independent techniques. Both techniques revealed a long term cycling of intracellular pH which has the same p... | [
-0.03084871545433998,
-0.049816474318504333,
0.039406776428222656,
-0.01943320408463478,
0.022971926257014275,
0.00005805807450087741,
0.0697900652885437,
0.02050149068236351,
-0.00008185346814570948,
-0.0019823338370770216,
-0.012282094918191433,
-0.12495122104883194,
0.031086673960089684,
... |
PMID:16938 | Evaluation of the Hall electrolytic conductivity detector for the analysis of narcotic alkaloid and phenothiazine drugs extracted from urine. | The electrolytic conductivity detector can be used for the rapid and selective determination of narcotic alkaloid and phenothiazine drugs in the presence of urine coextractants. The high selectivity of the detector enables drug concentrations as low as 0.1 mg/dl to be analyzed without extensive sample cleanup or deriva... | Evaluation of the Hall electrolytic conductivity detector for the analysis of narcotic alkaloid and phenothiazine drugs extracted from urine. The electrolytic conductivity detector can be used for the rapid and selective determination of narcotic alkaloid and phenothiazine drugs in the presence of urine coextractants. ... | [
-0.050397731363773346,
0.019856726750731468,
0.02189895324409008,
0.02541191317141056,
-0.024382514879107475,
-0.01747160218656063,
0.07898437976837158,
0.04147985577583313,
-0.05301780253648758,
-0.015988795086741447,
0.021749142557382584,
0.004333942662924528,
-0.02123512327671051,
0.038... |
PMID:16939 | Studies on the pathogenesis of an immune defect in multiple myeloma. | The reduced capacity of patients with multiple myeloma to respond to antigen challenge is well recognized. Response to antigen involves antigen recognition, cell proliferation, and synthesis and secretion of antibody. This study examines this sequence of events in peripheral blood lymphocytes from untreated and treated... | Studies on the pathogenesis of an immune defect in multiple myeloma. The reduced capacity of patients with multiple myeloma to respond to antigen challenge is well recognized. Response to antigen involves antigen recognition, cell proliferation, and synthesis and secretion of antibody. This study examines this sequence... | [
-0.03255176171660423,
0.018377939239144325,
-0.02371475100517273,
0.035692520439624786,
-0.025233954191207886,
-0.027962421998381615,
0.05420598387718201,
0.08062557876110077,
-0.04308713972568512,
-0.004796472378075123,
-0.0033316549379378557,
-0.034000806510448456,
0.0204553734511137,
0.... |
PMID:16940 | Aminoglycoside antibiotics and renal function: changes in urinary gamma-glutamyltransferase excretion. | The urinary excretion of the renal proximal tubular enzyme, gamma-glutamyltransferase (gamma-GT), has been studied in 41 patients receiving tobramycin, gentamicin or streptomycin for a variety of infections. All patients receiving tobramycin or gentamicin have shown increased excretion of gamma-GT in the urine. Only 46... | Aminoglycoside antibiotics and renal function: changes in urinary gamma-glutamyltransferase excretion. The urinary excretion of the renal proximal tubular enzyme, gamma-glutamyltransferase (gamma-GT), has been studied in 41 patients receiving tobramycin, gentamicin or streptomycin for a variety of infections. All patie... | [
0.026380473747849464,
-0.0185069777071476,
0.0429377481341362,
0.0006226608529686928,
0.012170253321528435,
-0.024474389851093292,
0.03430549055337906,
0.0613812580704689,
-0.03869839012622833,
-0.030995510518550873,
0.004551950842142105,
-0.015650780871510506,
0.05034211277961731,
0.07018... |
PMID:16943 | Effect of beta adrenergic agonist, prostaglandins, and cortisol on lymphocyte levels of cyclic adenosine monophosphate and glycogen: abnormal lymphocytic metabolism in asthma. | Decreased beta adrenergic regulation of cyclic adenosine monophosphate (cAMP) in lymphocytes has been described in asthma. We investigated adrenergic stimulation of glycogenolysis and responses to prostaglandin E1 (PGE1). Lymphocytes from 24 normal and 24 mild asthmatic subjects who had no drugs for at least 2 weeks we... | Effect of beta adrenergic agonist, prostaglandins, and cortisol on lymphocyte levels of cyclic adenosine monophosphate and glycogen: abnormal lymphocytic metabolism in asthma. Decreased beta adrenergic regulation of cyclic adenosine monophosphate (cAMP) in lymphocytes has been described in asthma. We investigated adren... | [
-0.01430534664541483,
-0.0004143294063396752,
0.0019521842477843165,
0.060389287769794464,
-0.031155474483966827,
-0.0076842643320560455,
0.06051390618085861,
-0.008969779126346111,
-0.051693085581064224,
-0.08009929955005646,
0.02462403103709221,
-0.01543695293366909,
0.015084086917340755,
... |
PMID:16944 | Further studies on the mechanism of human histamine-induced asthma: the effect of an aerosolized H1 receptor antagonist (diphenhydramine). | This study was designed to better define the mechanism of histamine-induced bronchoconstriction in humans by pharmacologic manipulation of the postulated bronchial histamine receptor sites. Histamine challenges were performed on a heterogeneous group of adult asthmatic subjects. The cumulative units of histamine requir... | Further studies on the mechanism of human histamine-induced asthma: the effect of an aerosolized H1 receptor antagonist (diphenhydramine). This study was designed to better define the mechanism of histamine-induced bronchoconstriction in humans by pharmacologic manipulation of the postulated bronchial histamine recepto... | [
-0.029363417997956276,
0.018322397023439407,
-0.015007931739091873,
0.06171984598040581,
-0.04011504724621773,
0.012668193317949772,
0.08514492958784103,
0.03729251027107239,
-0.07022933661937714,
-0.056701019406318665,
-0.006707960739731789,
-0.05530461668968201,
-0.0038010161370038986,
0... |
PMID:16945 | [Acid-base equilibrium during acute fetal hypoxia. Experimental study]. | The authors studied the effects of acute hypoxia of short duration in eight pregnant ewes that had been anaesthetised. They showed that during the experiment the E.E.G. as well as the pH and pCO and the maternal lactate levels were not changed and that therefore changes that can be found in the fetus are due solely to ... | [Acid-base equilibrium during acute fetal hypoxia. Experimental study]. The authors studied the effects of acute hypoxia of short duration in eight pregnant ewes that had been anaesthetised. They showed that during the experiment the E.E.G. as well as the pH and pCO and the maternal lactate levels were not changed and ... | [
-0.03467028960585594,
0.03369864076375961,
0.018889041617512703,
0.10611965507268906,
-0.0008114593802019954,
0.035560134798288345,
0.048208583146333694,
0.024675166234374046,
-0.01764838583767414,
0.0049761501140892506,
0.009961467236280441,
-0.05721018835902214,
-0.03195532411336899,
0.0... |
PMID:16972 | Enhancement of murine cytomegalovirus infection during graft-vs.-host reaction. | A mouse model was used for elucidation of the role of a graft-vs-host reaction in promoting cytomegalovirus infection. F1 (DBA/2 X C3H/He) hybrid mice were infected with murine cytomegalovirus. Five weeks later, a graft-vs.-host reaction was produced in the chronically infected animals by the administration of multiple... | Enhancement of murine cytomegalovirus infection during graft-vs.-host reaction. A mouse model was used for elucidation of the role of a graft-vs-host reaction in promoting cytomegalovirus infection. F1 (DBA/2 X C3H/He) hybrid mice were infected with murine cytomegalovirus. Five weeks later, a graft-vs.-host reaction wa... | [
-0.054383911192417145,
0.028103532269597054,
0.006099823396652937,
0.056649141013622284,
0.016861066222190857,
-0.010381445288658142,
0.0422021821141243,
0.14140796661376953,
-0.07024742662906647,
0.008421167731285095,
0.023593999445438385,
-0.011400389485061169,
-0.016784101724624634,
-0.... |
PMID:16976 | Protein binding to monosodium urate monohydrate, calcium pyrophosphate dihydrate, and silicon dioxide crystals. I. Physical characteristics. | The protein adsorptive properties of monosodium urate monohydrate (MSU), silica (Si), and calcium pyrophosphate dihydrate crystals were studied by qualitative and quantitative techniques. Immunoglobulin G (IgG) was adsorbed preferentially by MSU crystals from normal human serum and demonstrated high-affinity binding is... | Protein binding to monosodium urate monohydrate, calcium pyrophosphate dihydrate, and silicon dioxide crystals. I. Physical characteristics. The protein adsorptive properties of monosodium urate monohydrate (MSU), silica (Si), and calcium pyrophosphate dihydrate crystals were studied by qualitative and quantitative tec... | [
-0.09037475287914276,
0.0019079155754297972,
-0.0007293866947293282,
-0.007572128437459469,
0.0012669010320678353,
-0.02081427164375782,
0.10248289257287979,
0.048013050109148026,
-0.06128423288464546,
0.02768530324101448,
0.009139793924987316,
-0.06845948845148087,
0.0037774965167045593,
... |
PMID:16977 | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions. | An investigation of the effect of change of total CO(2) concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-(14)C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-(14)C]glucose into phosphatidylcholine and phosphatidylglycerol of the surfa... | Effect of CO2 concentration on phosphatidylcholine and phosphatidylglycerol metabolism in surfactant and residual lung fractions. An investigation of the effect of change of total CO(2) concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-(14)C]glucose incorporation into lung phosph... | [
-0.05469812452793121,
-0.015964113175868988,
0.008294342085719109,
0.03215079382061958,
-0.022364608943462372,
-0.009887189604341984,
0.031354207545518875,
-0.006576653104275465,
-0.04516177251935005,
-0.005811531562358141,
0.0023898964282125235,
-0.07810065895318985,
-0.016616275534033775,
... |
PMID:16978 | The significance of lipoprotein lipase in rat skeletal muscles. | Lipoprotein lipase was assayed in extracts of acetone-ether powders of rat skeletal muscles. Enzyme activity in soleus had typical characteristics of lipoprotein lipase in other tissues: inhibition by molar NaCl and protamine sulfate and activation by the human apolipoprotein, R-glutamic acid. Activity in muscles with ... | The significance of lipoprotein lipase in rat skeletal muscles. Lipoprotein lipase was assayed in extracts of acetone-ether powders of rat skeletal muscles. Enzyme activity in soleus had typical characteristics of lipoprotein lipase in other tissues: inhibition by molar NaCl and protamine sulfate and activation by the ... | [
-0.030022455379366875,
-0.006439403165131807,
-0.028819898143410683,
0.027334867045283318,
0.024543553590774536,
-0.035561468452215195,
0.05323265492916107,
0.07170242816209793,
-0.01232432946562767,
-0.03333716467022896,
0.027568047866225243,
-0.10182693600654602,
-0.04829957336187363,
0.... |
PMID:16979 | Endocrine regulation of sex-dependent hydroxysteroid dehydrogenase activities in rat kidney: NADP-dependent microsomal 3alpha- and 20beta-hydroxysteroid dehydrogenase. | The NADP-dependent microsomal kidney enzymes, 3alpha- and 20beta-hydroxysteroid dehydrogenase (HSDH), which exhibit considerable sex differences in their activities (male:female activity ratios, 16:1 and 30:1 respectively), were investigated after interference with the pituitary-gonad and pituitary-adrenal systems. Pre... | Endocrine regulation of sex-dependent hydroxysteroid dehydrogenase activities in rat kidney: NADP-dependent microsomal 3alpha- and 20beta-hydroxysteroid dehydrogenase. The NADP-dependent microsomal kidney enzymes, 3alpha- and 20beta-hydroxysteroid dehydrogenase (HSDH), which exhibit considerable sex differences in thei... | [
0.023820262402296066,
0.0094953253865242,
0.0031783445738255978,
0.000015476123735425062,
-0.012461617588996887,
-0.0015260461950674653,
0.04673595353960991,
0.009800094179809093,
-0.04646052420139313,
-0.02690849080681801,
0.014476533979177475,
-0.04992403835058212,
0.04113186150789261,
0... |
PMID:16980 | [A comparision of different methods and reagents for the determination of gamma-glutamyl transferase in serum (author's transl)]. | Four commercially available kinetic photometric tests for the determination of gamma-glutamyl transferase were compared. Test A is based on the substrate L-gamma-glutamyl-4-nitroanilide; test B uses this same substrate dissolved in hydrochloric acid. C1 and C2 are tests from different suppliers; both use the substrate ... | [A comparision of different methods and reagents for the determination of gamma-glutamyl transferase in serum (author's transl)]. Four commercially available kinetic photometric tests for the determination of gamma-glutamyl transferase were compared. Test A is based on the substrate L-gamma-glutamyl-4-nitroanilide; tes... | [
-0.000951651600189507,
0.0004090801812708378,
-0.0018499643774703145,
0.037068866193294525,
0.05199010297656059,
0.018397483974695206,
0.06349830329418182,
0.05859455466270447,
-0.046328067779541016,
-0.025613732635974884,
0.03173767030239105,
-0.046426255255937576,
-0.003846440464258194,
... |
PMID:16982 | Exchange diffusion of dopamine induced in planar lipid bilayer membranes by the ionophore X537A. | The ionophore X537A causes a large increase in the [(14)C]dopamine (a catecholamine) permeability of planar bilayer membranes. Dopamine transport increases linearly with the ionophore concentration. At relatively high concentrations in the presence of dopamine, the ionophore omdices a conductance which is nearly ideall... | Exchange diffusion of dopamine induced in planar lipid bilayer membranes by the ionophore X537A. The ionophore X537A causes a large increase in the [(14)C]dopamine (a catecholamine) permeability of planar bilayer membranes. Dopamine transport increases linearly with the ionophore concentration. At relatively high conce... | [
-0.05689429119229317,
-0.04600847139954567,
0.015254816971719265,
0.09665996581315994,
-0.026150919497013092,
0.005282674450427294,
0.09391900151968002,
0.04151754081249237,
-0.043219100683927536,
-0.048803504556417465,
0.022058196365833282,
-0.08526971191167831,
-0.027056703343987465,
0.0... |
PMID:16983 | The rumen flagellate Piromonas communis: its life-history and invasion of plant material in the rumen. | The rumen flagellat Piromonas communis is the zoospore of a phycomycete fungus inhabiting the rumen. Zoosporogenesis was stimulated by a dietary component (the inducer), and inhibited by compounds affecting membrane structure and function, but not by inhibitors of protein synthesis. The zoospores showed taxis towards t... | The rumen flagellate Piromonas communis: its life-history and invasion of plant material in the rumen. The rumen flagellat Piromonas communis is the zoospore of a phycomycete fungus inhabiting the rumen. Zoosporogenesis was stimulated by a dietary component (the inducer), and inhibited by compounds affecting membrane s... | [
0.050710294395685196,
-0.02862192690372467,
0.020743366330862045,
0.024167800322175026,
0.0817563608288765,
-0.021924283355474472,
-0.01714441552758217,
0.038496751338243484,
-0.01047917827963829,
0.04085422679781914,
-0.004148258827626705,
-0.10953137278556824,
0.010450954549014568,
0.005... |
PMID:16991 | The effects of beta adrenergic blockade on spinal cord autoregulation in the monkey. | Blood flow in the spinal cord was measured in a group of monkeys over a wide range of artificially varied blood pressures after the administration of propranolol, a beta adrenergic blocker. Spinal cord blood flow was found to be constant and in the normal range between a mean system arterial blood pressure of 50 to 150... | The effects of beta adrenergic blockade on spinal cord autoregulation in the monkey. Blood flow in the spinal cord was measured in a group of monkeys over a wide range of artificially varied blood pressures after the administration of propranolol, a beta adrenergic blocker. Spinal cord blood flow was found to be consta... | [
-0.03184164687991142,
0.003270527347922325,
-0.035259876400232315,
0.019538788124918938,
0.0184076689183712,
0.02209790237247944,
0.054846733808517456,
0.04177052155137062,
-0.003426350187510252,
-0.05147755146026611,
0.04806342348456383,
-0.002492124680429697,
-0.012326774187386036,
0.071... |
PMID:16993 | In vivo effects of glucagon on fatty acid synthesis in fasted and refed rats. | Since altered nutritional states evoke compensatory changes in systemic levels of several hormones, the present study was conducted to determine in vivo effects of glucagon and insulin on hepatic and adipose tissue lipogenesis in fed, fasted (3-days) and refed (3-days) rats. Compared to the fed controls, glucagon reduc... | In vivo effects of glucagon on fatty acid synthesis in fasted and refed rats. Since altered nutritional states evoke compensatory changes in systemic levels of several hormones, the present study was conducted to determine in vivo effects of glucagon and insulin on hepatic and adipose tissue lipogenesis in fed, fasted ... | [
0.032730765640735626,
-0.005970025435090065,
0.04747670516371727,
0.07182364165782928,
0.00640861177816987,
-0.015317984856665134,
-0.008288106881082058,
0.053033750504255295,
-0.0936456024646759,
-0.029812505468726158,
-0.00805747415870428,
-0.05983946844935417,
0.020808430388569832,
0.04... |
PMID:16995 | Effects of putative neurotransmitters on the motor activity of Spirometra mansonoides. | 5-Hydroxytryptamine (5-HT), epinephrine, and dopamine strongly stimulated the motor activity of larval Spirometra mansonoides. By contrast, a cholinomimetic agent, arecoline, paralyzed the worms. There was some pharmacological specificity among the agonists but not with various antagonists. Acetylcholinesterase activit... | Effects of putative neurotransmitters on the motor activity of Spirometra mansonoides. 5-Hydroxytryptamine (5-HT), epinephrine, and dopamine strongly stimulated the motor activity of larval Spirometra mansonoides. By contrast, a cholinomimetic agent, arecoline, paralyzed the worms. There was some pharmacological specif... | [
0.009088105522096157,
-0.04993981122970581,
0.006673815194517374,
-0.00608420092612505,
-0.07012350112199783,
-0.03074001334607601,
0.09839962422847748,
0.07947799563407898,
-0.05126059055328369,
-0.025598008185625076,
0.05399724841117859,
-0.009443103335797787,
-0.03331925719976425,
0.037... |
PMID:16997 | Actions of iontophoretic phenytoin and medazepam on hippocampal neurons. | In rats anesthetized with methoxyflurane, phenytoin (DPH) and medazepam (MDZ) were administered iontophoretically to pyramidal and granule cells discharging spontaneously or being driven by acetylcholine or glutamic acid. The objectives were to determine if: 1) these anticonvulsant agents exert direct effects on the ra... | Actions of iontophoretic phenytoin and medazepam on hippocampal neurons. In rats anesthetized with methoxyflurane, phenytoin (DPH) and medazepam (MDZ) were administered iontophoretically to pyramidal and granule cells discharging spontaneously or being driven by acetylcholine or glutamic acid. The objectives were to de... | [
-0.06366905570030212,
0.03070637583732605,
0.009665185585618019,
0.007743330672383308,
-0.01910358853638172,
0.045007381588220596,
0.07673530280590057,
0.026174301281571388,
-0.008561810478568077,
-0.040186140686273575,
-0.004255367908626795,
0.005019882693886757,
0.0711405873298645,
0.044... |
PMID:16998 | Unusual alpha adrenergic receptor potency of methyldopa metabolites on melanocyte function. | Catecholamines possessing alpha adrenergic receptor agonist properties induce lightening or reverse melanocyte stimulating hormone darkening of frog skin in vitro. The capacity to activate this alpha receptor by the methyldopa metabolites methyldopamine and methylnorepinephrine was compared with the capacity of the nat... | Unusual alpha adrenergic receptor potency of methyldopa metabolites on melanocyte function. Catecholamines possessing alpha adrenergic receptor agonist properties induce lightening or reverse melanocyte stimulating hormone darkening of frog skin in vitro. The capacity to activate this alpha receptor by the methyldopa m... | [
-0.053292591124773026,
-0.028122393414378166,
-0.004855239763855934,
0.04409606009721756,
-0.0224451944231987,
0.010263176634907722,
0.07489760220050812,
0.03217729926109314,
-0.0360674224793911,
-0.020886395126581192,
-0.0008138227858580649,
-0.03795495629310608,
-0.047338493168354034,
0.... |
PMID:17000 | Alpha blocking action of the antihypertensive agent, prazosin. | The vasodilatory and alpha adrenergic blocking properties of prazosin were studied in anesthetized rats and compared with the direct-acting vasodilator, diazoside. The hypotensive activity of diazoxide was unimpaired after ganglion blockade with pentolinium or alpha adrenoreceptor blockade with phentolamine; diazoxide ... | Alpha blocking action of the antihypertensive agent, prazosin. The vasodilatory and alpha adrenergic blocking properties of prazosin were studied in anesthetized rats and compared with the direct-acting vasodilator, diazoside. The hypotensive activity of diazoxide was unimpaired after ganglion blockade with pentolinium... | [
-0.05472835525870323,
0.02806740626692772,
0.009802525863051414,
0.03019873984158039,
0.02714359201490879,
-0.030164359137415886,
0.07075109332799911,
0.009554621763527393,
-0.03167122229933739,
-0.08215302228927612,
-0.014928408898413181,
-0.00035018110065720975,
-0.041651781648397446,
0.... |
PMID:17001 | Measurement of prostaglandin E2 in an inflammatory exudate: effects of nonsteroidal anti-inflammatory agents. | A method was developed for extracting and measuring nanogram quantities of prostaglandin E2 (PGE2) from carrageenan-induced abscess in the rat. PGE2 concentration, quantitated by radioimmunoassay, was 42.5 and 92.9 ng/g of abscess in two studies. Anti-inflammatory activity, based on reduction in abscess weight, was obs... | Measurement of prostaglandin E2 in an inflammatory exudate: effects of nonsteroidal anti-inflammatory agents. A method was developed for extracting and measuring nanogram quantities of prostaglandin E2 (PGE2) from carrageenan-induced abscess in the rat. PGE2 concentration, quantitated by radioimmunoassay, was 42.5 and ... | [
-0.030821122229099274,
-0.001483144355006516,
-0.018458249047398567,
-0.008284778334200382,
0.007786171976476908,
-0.006311942357569933,
0.03746829926967621,
0.06037488207221031,
-0.04307809844613075,
-0.00434190034866333,
0.05955282971262932,
-0.054709985852241516,
0.04782133549451828,
0.... |
PMID:17002 | The concentration of ionized magnesium in barnacle muscle fibres. | 1. The total Mg in isolated fibres of Balanus aquila was 10-5 m-mole/kg wet wt. 2. The intracellular free Mg was measured by a null point method using Eriochrome Blue as an indicator of free Mg, and internal dialysis with solutions of varying ionized Mg concentrations. The results indicated a free Mg of 6 mM or 4-2 m-m... | The concentration of ionized magnesium in barnacle muscle fibres. 1. The total Mg in isolated fibres of Balanus aquila was 10-5 m-mole/kg wet wt. 2. The intracellular free Mg was measured by a null point method using Eriochrome Blue as an indicator of free Mg, and internal dialysis with solutions of varying ionized Mg ... | [
-0.020131314173340797,
-0.04595228657126427,
0.03619629144668579,
-0.0033414363861083984,
0.03958422690629959,
-0.06344860792160034,
0.0851392075419426,
0.054230134934186935,
-0.02508707344532013,
0.032327815890312195,
0.042998652905225754,
-0.13921140134334564,
-0.017832783982157707,
0.01... |
PMID:17003 | Separative pathways for urea and water, and for chloride in chicken erythrocytes. | 1. Urea and water permeabilities of chicken erythrocytes are considerably lower than those of mammalian red cells. 2. The permeabilities to urea, thiourea and to N-methylurea (about 10(-6) cm/sec at 25 degrees C) were independent of concentration within a very broad range, and we found no evidence of interaction betwee... | Separative pathways for urea and water, and for chloride in chicken erythrocytes. 1. Urea and water permeabilities of chicken erythrocytes are considerably lower than those of mammalian red cells. 2. The permeabilities to urea, thiourea and to N-methylurea (about 10(-6) cm/sec at 25 degrees C) were independent of conce... | [
-0.08216608315706253,
-0.10684739053249359,
-0.015830757096409798,
0.03842638432979584,
0.02981262467801571,
0.004524388816207647,
0.06688069552183151,
0.01431707851588726,
0.00926183257251978,
0.009385943412780762,
-0.016084302216768265,
-0.10269567370414734,
0.001278039300814271,
-0.0062... |
PMID:17004 | Cell size, macromolecular composition, and O2 consumption during agitated cultivation of Naegleria gruberi. | Cell size, macromolecular composition, carbohydrate utilization patterns, and O2 concentrations were measured throughout the growth stages of Naegleria gruberi in agitated culture in a complex medium. Biphasic logarithmic growth occurred during the intial 83 hr of growth and the mean generation time was 7.0 hr and 19 h... | Cell size, macromolecular composition, and O2 consumption during agitated cultivation of Naegleria gruberi. Cell size, macromolecular composition, carbohydrate utilization patterns, and O2 concentrations were measured throughout the growth stages of Naegleria gruberi in agitated culture in a complex medium. Biphasic lo... | [
-0.018760833889245987,
0.019119465723633766,
-0.020009424537420273,
-0.006535989232361317,
0.03282918408513069,
-0.015998346731066704,
0.006998240016400814,
0.0011905626161023974,
-0.038336992263793945,
0.027528438717126846,
0.05826224386692047,
-0.08743981271982193,
0.0289781354367733,
0.... |
PMID:17006 | Use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport. | The interaction of 1-anilino-8-naphthalene-sulfonate (ANS) with vesicles derived from hog fundic mucosa was studied in the presence of valinomycin and with the addition of ATP. Evidence was found for two classes of sites, those rapidly accessible to ANS with a KD of 7.5 micronM and those slowly accessible, but rapidly ... | Use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport. The interaction of 1-anilino-8-naphthalene-sulfonate (ANS) with vesicles derived from hog fundic mucosa was studied in the presence of valinomycin and with the addition of ATP. Evidence was found for two classes of sites, those rapidly ac... | [
-0.0724371075630188,
-0.039958808571100235,
-0.013506822288036346,
0.011759715154767036,
-0.027399394661188126,
0.008741267025470734,
0.03635018318891525,
-0.014443635009229183,
-0.05710727721452713,
-0.026783086359500885,
0.005592493340373039,
-0.0387219563126564,
0.008139356970787048,
0.... |
PMID:17007 | Cation transport by gastric H+:K+ ATPase. | A vesicular microsomal fraction isolated from hog fundic mucosa demonstrates the capacity to take up equal amounts of RB+ and Cl-. The amount of the Rb+ uptake is sensitive to the extravesicular osmolarity, and rate of uptake is sensitive to temperature. 86Rb+ efflux is dependent upon the cation composition of the dilu... | Cation transport by gastric H+:K+ ATPase. A vesicular microsomal fraction isolated from hog fundic mucosa demonstrates the capacity to take up equal amounts of RB+ and Cl-. The amount of the Rb+ uptake is sensitive to the extravesicular osmolarity, and rate of uptake is sensitive to temperature. 86Rb+ efflux is depende... | [
-0.08090894669294357,
-0.03599533438682556,
0.013882113620638847,
0.0244912039488554,
0.03951768949627876,
-0.035791944712400436,
0.02675246261060238,
0.03479940444231033,
-0.049448270350694656,
-0.03930813819169998,
-0.0029503917321562767,
-0.05530264601111412,
-0.031958337873220444,
-0.0... |
PMID:17008 | Characterization of bile acid binding to rat intestinal brush border membranes. | Studies were performed to characterize the binding1 of bile acids to intestinal brush border membranes. Total 14C-taurodeoxycholate binding was: 1) similar for brush borders prepared from jejunum and ileum, 2) linear with respect to monomer concentration, 3) uninhibited by a structural analog, and 4) not depressed by b... | Characterization of bile acid binding to rat intestinal brush border membranes. Studies were performed to characterize the binding1 of bile acids to intestinal brush border membranes. Total 14C-taurodeoxycholate binding was: 1) similar for brush borders prepared from jejunum and ileum, 2) linear with respect to monomer... | [
-0.014064420945942402,
-0.03344222903251648,
0.016217658296227455,
0.0018751545576378703,
-0.04877108335494995,
-0.039399709552526474,
0.07424800843000412,
0.06277307868003845,
-0.004085440654307604,
-0.012133135460317135,
0.03533651679754257,
-0.10130524635314941,
0.04581215977668762,
-0.... |
PMID:17009 | Purple membrane vesicles: morphology and proton translocation. | Purple membrane vesicles prepared by different techniques differ widely in their morphology and ability to establish a proton gradient in the light. The procedures used to prepare active vesicles do not completely dissociate the purple membrane and thus preserve a preferential orientation of the protein, while most of ... | Purple membrane vesicles: morphology and proton translocation. Purple membrane vesicles prepared by different techniques differ widely in their morphology and ability to establish a proton gradient in the light. The procedures used to prepare active vesicles do not completely dissociate the purple membrane and thus pre... | [
-0.09602414071559906,
-0.04364284127950668,
-0.02823621779680252,
0.013281156308948994,
-0.016909103840589523,
-0.014931946061551571,
0.05639718472957611,
0.00988720078021288,
-0.023751748725771904,
0.03207451105117798,
0.04413185641169548,
-0.09568099677562714,
0.008817695081233978,
0.026... |
PMID:17014 | Effect of pH on hyperthermic cell survival. | Chinese hamster ovary cells incubated with various concentrations of CO2, to obtain extracellular pH values in the range of 6.40-7.85, were heated at 45.5C for 5, 10, or 20 minutes. Thermal sensitivity increased sharply from pH 7.35 to 6.65 (i.e., survival decreased from 1 X 10(-2) to 3 X 10(-5) for 20 minutes of heati... | Effect of pH on hyperthermic cell survival. Chinese hamster ovary cells incubated with various concentrations of CO2, to obtain extracellular pH values in the range of 6.40-7.85, were heated at 45.5C for 5, 10, or 20 minutes. Thermal sensitivity increased sharply from pH 7.35 to 6.65 (i.e., survival decreased from 1 X ... | [
-0.06541569530963898,
0.038706932216882706,
0.056721173226833344,
0.04788436368107796,
0.06077825278043747,
-0.0012659032363444567,
0.06256776303052902,
0.019166043028235435,
-0.0386686772108078,
-0.035829804837703705,
0.04018254205584526,
-0.06796703487634659,
0.022309087216854095,
0.0436... |
PMID:17015 | Aryl and heterocyclic diazo compounds as potential environmental electrophiles. | 4-Aminoimidazole-5-carboxamide, a component of human urine derived from the de novo purine biosynthetic pathway, was evidenced to undergo in vivo diazotization in rats following its sequential administration with NaNO2. The diazotization product, 4-diazoimidazole-5-carboxamide, undergoes intramolecular cyclization to y... | Aryl and heterocyclic diazo compounds as potential environmental electrophiles. 4-Aminoimidazole-5-carboxamide, a component of human urine derived from the de novo purine biosynthetic pathway, was evidenced to undergo in vivo diazotization in rats following its sequential administration with NaNO2. The diazotization pr... | [
-0.057812150567770004,
-0.015792300924658775,
0.01524378638714552,
-0.00657960120588541,
0.05326300114393234,
-0.023360610008239746,
0.01448146067559719,
0.003546531079337001,
-0.09656382352113724,
-0.008244176395237446,
0.03099551796913147,
0.005154712591320276,
0.010962558910250664,
0.01... |
PMID:17017 | Mosquito transmission of wild turkey malaria, Plasmodium hermani. | Culex nigripalpus experimentally transmitted Plasmodium hermani, a plasmodium of wild turkeys (Meleagris gallopavo) in Florida. The mosquitoes were infected by feeding upon blood induced parasitemias in domestic turkey poults. The resulting sporozoites, transmitted by either mosquito bites or injection, produced malari... | Mosquito transmission of wild turkey malaria, Plasmodium hermani. Culex nigripalpus experimentally transmitted Plasmodium hermani, a plasmodium of wild turkeys (Meleagris gallopavo) in Florida. The mosquitoes were infected by feeding upon blood induced parasitemias in domestic turkey poults. The resulting sporozoites, ... | [
0.035125188529491425,
-0.015335152857005596,
0.028959060087800026,
0.015788374468684196,
0.08595148473978043,
-0.032693177461624146,
0.09017271548509598,
0.08729090541601181,
0.0017154384404420853,
-0.00356378685683012,
-0.038198888301849365,
-0.050905901938676834,
-0.014985340647399426,
0... |
PMID:17018 | Nosocomial bacteremia. Potential for prevention of procedure-related cases. | During a six-month period, 187 inpatients had bacteremia associated with community-acquired infection and 91 patients had bacteremia from a nosocomial infection. The most frequently identified sites of infection in both types of bacteremia were the respiratory and urinary tracts. Escherichia coli and Diplococcus pneumo... | Nosocomial bacteremia. Potential for prevention of procedure-related cases. During a six-month period, 187 inpatients had bacteremia associated with community-acquired infection and 91 patients had bacteremia from a nosocomial infection. The most frequently identified sites of infection in both types of bacteremia were... | [
-0.04858008027076721,
0.03224002197384834,
-0.03577321395277977,
0.026921264827251434,
0.07389004528522491,
-0.05153718590736389,
0.03288554772734642,
0.04638845846056938,
-0.02412307821214199,
0.010514569468796253,
-0.04500531405210495,
0.004122063983231783,
0.019539600238204002,
0.020096... |
PMID:17020 | Costs and outcomes for different primary care providers. | This study examined the relationship between levels of medical training and direct costs for 1,700 episodes of acute illness treated in ambulatory-care clinics. Faculty, family practice residents, and physician assistants were included as the providers. Total cost and four component costs were examined. An outcome was ... | Costs and outcomes for different primary care providers. This study examined the relationship between levels of medical training and direct costs for 1,700 episodes of acute illness treated in ambulatory-care clinics. Faculty, family practice residents, and physician assistants were included as the providers. Total cos... | [
-0.010641445405781269,
-0.012079710140824318,
0.004438797943294048,
-0.026330428197979927,
0.04488731175661087,
-0.020613117143511772,
0.023503657430410385,
0.04151780530810356,
0.015089457854628563,
-0.040337659418582916,
0.0467454232275486,
0.002102762460708618,
-0.00507024209946394,
0.0... |
PMID:17021 | [In vitro examination on antibacterial activity of ciclacillin (ACPC) against clinically isolated strains (author's transl)]. | (1) The antibacterial acivity of ciclacillin (ACPC) with inoculum size of 10(6) cells/ml was four times less potent than that of ampicillin (ABPC) and 4 approximately 8 times less potent than that of AMPC, but was 4 approximately 8 times more potent than that of CEX against Streptococcus pyogenes. For Streptococcus pne... | [In vitro examination on antibacterial activity of ciclacillin (ACPC) against clinically isolated strains (author's transl)]. (1) The antibacterial acivity of ciclacillin (ACPC) with inoculum size of 10(6) cells/ml was four times less potent than that of ampicillin (ABPC) and 4 approximately 8 times less potent than th... | [
-0.05140005797147751,
-0.02215123176574707,
-0.018697170540690422,
0.058673880994319916,
0.041221730411052704,
-0.011174765415489674,
0.005557089578360319,
0.08315398544073105,
-0.05156334489583969,
-0.007963721640408039,
0.013161249458789825,
-0.03449154645204544,
0.02350323647260666,
-0.... |
PMID:17025 | Effects of metiamide and propranolol on gastric secretion in anesthetized dogs. | The effects of metiamide, a histamine H2-receptor antagonist, and propranolol, a beta-adrenergic blocking agent, on gastric secretion were studied in anesthetized dogs. Metiamide, 1.45 mg/kg i.v., markedly inhibited the gastric secretion induced by a continuous i.v. infusion of tetragastrin (8 microng/kg-hr), histamine... | Effects of metiamide and propranolol on gastric secretion in anesthetized dogs. The effects of metiamide, a histamine H2-receptor antagonist, and propranolol, a beta-adrenergic blocking agent, on gastric secretion were studied in anesthetized dogs. Metiamide, 1.45 mg/kg i.v., markedly inhibited the gastric secretion in... | [
0.0066628223285079,
0.0015470227226614952,
-0.0038625162560492754,
0.06996291875839233,
-0.008850134909152985,
0.006483832374215126,
0.04375181347131729,
-0.0052362908609211445,
-0.04910345748066902,
-0.06418094784021378,
0.0022029157262295485,
-0.03646719083189964,
0.023558955639600754,
0... |
PMID:17026 | Descending release of acetylcholine from the locally distended guinea pig ileum. | The effects of local distension of the intestinal wall on the release of acetylcholine (ACh) from the adjacent non-distended part were studied with the segment os isolated guinea pig ileum. Local distension of the intestinal wall induced the increased release of ACh in the distended part and in its anal side but not in... | Descending release of acetylcholine from the locally distended guinea pig ileum. The effects of local distension of the intestinal wall on the release of acetylcholine (ACh) from the adjacent non-distended part were studied with the segment os isolated guinea pig ileum. Local distension of the intestinal wall induced t... | [
-0.009877624921500683,
0.012552294880151749,
0.03314468264579773,
0.04654364287853241,
0.004360190127044916,
-0.021930044516921043,
0.004208968952298164,
0.02683659829199314,
-0.01898846961557865,
-0.08528771996498108,
-0.014206130988895893,
-0.06829023361206055,
-0.03828079625964165,
0.00... |
PMID:17028 | Purification of histamine receptor. (IV) Specificity of binding of various drugs to the histamine receptor-rich fraction and to solubilized binding sites. | Studies were made on tritiated histamine binding to the receptor-rich membrane fraction and solubilized sites and its displacement by various drugs. H1-Agonists and antagonists displaced histamine most effectively. A H2-agonist and atropine were less effective and propranolol, phentolamine and imidazole acetic acid had... | Purification of histamine receptor. (IV) Specificity of binding of various drugs to the histamine receptor-rich fraction and to solubilized binding sites. Studies were made on tritiated histamine binding to the receptor-rich membrane fraction and solubilized sites and its displacement by various drugs. H1-Agonists and ... | [
-0.04276690259575844,
0.008424658328294754,
-0.042606569826602936,
0.016539916396141052,
-0.03098536655306816,
-0.07871269434690475,
0.07910802960395813,
0.021970154717564583,
-0.060178324580192566,
-0.03414446488022804,
-0.006150607485324144,
-0.047134123742580414,
0.034319475293159485,
0... |
PMID:17031 | [Acquired colour-vision-deficiencies caused by side-effects of pharmacotherapy (author's transl)]. | Acquired colour-vision deficiencies are an early indicator for drug-induced retinopathy as well as drug-induced retrobulbar neuritis. Koellner's rule, which says, that damage of the retina induces a tritan-defect, and damage of the optic nerve induce a red-green-defect is also valid for defects secondary to drug-toxici... | [Acquired colour-vision-deficiencies caused by side-effects of pharmacotherapy (author's transl)]. Acquired colour-vision deficiencies are an early indicator for drug-induced retinopathy as well as drug-induced retrobulbar neuritis. Koellner's rule, which says, that damage of the retina induces a tritan-defect, and dam... | [
-0.05103404447436333,
-0.01682981103658676,
0.02772172912955284,
0.01645039953291416,
0.010221360251307487,
0.002574816346168518,
0.0703909769654274,
0.0910663828253746,
-0.09811180830001831,
-0.022407598793506622,
0.01900668442249298,
-0.04202696681022644,
0.027393324300646782,
0.07408247... |
PMID:17032 | beta-Adrenergic receptors in rat kidney: direct localization by a fluorescent beta-blocker. | A new fluorescent beta-adrenergic blocker, 9-amino-acridin propranolol (9-AAP), was administered intravenously to albino rats. Fluorescent aggregates of 9-AAP binding sites were concentrated in the vascular poles of the glomeruli in association with the preglomerular afferent arterioles. 9-AAP fluorescence was reduced ... | beta-Adrenergic receptors in rat kidney: direct localization by a fluorescent beta-blocker. A new fluorescent beta-adrenergic blocker, 9-amino-acridin propranolol (9-AAP), was administered intravenously to albino rats. Fluorescent aggregates of 9-AAP binding sites were concentrated in the vascular poles of the glomerul... | [
-0.04169875383377075,
-0.021518263965845108,
-0.030184553936123848,
-0.0034324289299547672,
-0.01740574836730957,
0.0031160435173660517,
0.05917463079094887,
0.027432721108198166,
-0.04163121432065964,
-0.023929506540298462,
0.009572774171829224,
-0.010874715633690357,
-0.02774236537516117,
... |
PMID:17033 | Ultrastructural localization of calcium in normal and abnormal skeletal muscle. | Calcium was demonstrated ultrastructurally as a fine black reaction product with unbuffered 2% saturated potassium pyroantimonate, pH 9.4. In comparison with normal muscle, there was increased precipitate in degenerating skeletal muscle fibers and some degenerating-regenerating fibers occurring in pathologic human musc... | Ultrastructural localization of calcium in normal and abnormal skeletal muscle. Calcium was demonstrated ultrastructurally as a fine black reaction product with unbuffered 2% saturated potassium pyroantimonate, pH 9.4. In comparison with normal muscle, there was increased precipitate in degenerating skeletal muscle fib... | [
-0.02585865929722786,
-0.051279690116643906,
0.014019585214555264,
-0.005701002664864063,
0.020858673378825188,
-0.04394754394888878,
0.03377273306250572,
-0.007823142223060131,
0.027899738401174545,
-0.024720167741179466,
0.05104401335120201,
-0.08078201860189438,
-0.02303113043308258,
0.... |
PMID:17038 | [Prolongated amnesia after "rohypnol" i.v. before local anesthesia and responsiveness during operation (author's transl)]. | An up-to-eight-hour lasting anterograde amnesia is achieved by "Rohypnol" i.v. causing no excitation before local anesthesia is given (prolongation by analgetics, anesthetics, neuroleptics). The cardiovascular functions remain stable with spontaneous breathing and preservation of the swallow and coughing reflexes. Duri... | [Prolongated amnesia after "rohypnol" i.v. before local anesthesia and responsiveness during operation (author's transl)]. An up-to-eight-hour lasting anterograde amnesia is achieved by "Rohypnol" i.v. causing no excitation before local anesthesia is given (prolongation by analgetics, anesthetics, neuroleptics). The ca... | [
-0.020353052765130997,
0.0049932063557207584,
0.024320513010025024,
0.009014658629894257,
-0.06868650764226913,
0.01305960863828659,
0.06596971303224564,
0.03881547227501869,
-0.033213499933481216,
-0.05766579136252403,
-0.05573923513293266,
0.04507161304354668,
-0.007527925074100494,
0.01... |
PMID:17048 | Effect of chlorhexidine on plaque development in an artificial mouth. | The effect of chlorhexidine on the development of plaque, resulting from the inoculation with saliva of a tooth mounted in an artificial mouth, has been studied. The agent delayed the formation of plaque and also inhibited changes in pH and Eh, whether sucrose was present or not. Applied as a rinse at intervals of 12 h... | Effect of chlorhexidine on plaque development in an artificial mouth. The effect of chlorhexidine on the development of plaque, resulting from the inoculation with saliva of a tooth mounted in an artificial mouth, has been studied. The agent delayed the formation of plaque and also inhibited changes in pH and Eh, wheth... | [
-0.04428385570645332,
-0.012001070193946362,
0.05528240278363228,
0.052279070019721985,
-0.032269611954689026,
-0.004549040924757719,
0.02642855979502201,
0.04389882832765579,
-0.04180031642317772,
-0.004177392460405827,
-0.011511317454278469,
-0.07268847525119781,
0.0644991397857666,
0.02... |
PMID:17049 | A microcalorimetric study of the growth of Klebsiella aerogenes in simple salts/glucose media. | Heat output-time records or 'thermograms' produced during the aerobic growth of Klebsiella aerogenes in simple salts/glucose media with growth limiting glucose concentrations of 2.0, 1.0 and 0.5 g dm-3 were obtained using a flow-microcalorimeter fitted with an aerobic cell. These traces are interpreted in terms of the ... | A microcalorimetric study of the growth of Klebsiella aerogenes in simple salts/glucose media. Heat output-time records or 'thermograms' produced during the aerobic growth of Klebsiella aerogenes in simple salts/glucose media with growth limiting glucose concentrations of 2.0, 1.0 and 0.5 g dm-3 were obtained using a f... | [
-0.01330220140516758,
0.03989221528172493,
0.03192383423447609,
0.025408882647752762,
0.07073959708213806,
-0.009637841023504734,
-0.031571969389915466,
-0.003434070385992527,
0.005471234209835529,
0.01624027080833912,
0.005656983703374863,
-0.06596475094556808,
0.02218513749539852,
0.0147... |
PMID:17065 | Studies of yeast alcohol dehydrogenase with 3-aminopyridine monucleotide. | 3-Aminopyridine mononucleotide, a nicotinamide mononucleotide analog, was prepared by enzymatic cleavage of 3-aminopyridine adenine dinucleotide by a snake venom phosphodiesterase and isolated by means of ion exchange chromatography. The spectrophotometric and fluorometric properties of this analog were studied. Severa... | Studies of yeast alcohol dehydrogenase with 3-aminopyridine monucleotide. 3-Aminopyridine mononucleotide, a nicotinamide mononucleotide analog, was prepared by enzymatic cleavage of 3-aminopyridine adenine dinucleotide by a snake venom phosphodiesterase and isolated by means of ion exchange chromatography. The spectrop... | [
-0.05948255583643913,
0.013411439023911953,
-0.031213155016303062,
0.02281629852950573,
0.02918689325451851,
-0.016178354620933533,
0.04751463606953621,
0.05806602165102959,
-0.061612360179424286,
-0.004549295175820589,
0.058489181101322174,
-0.04498037323355675,
-0.036240510642528534,
-0.... |
PMID:17066 | [Vaccination complications after oral poliomyelitis vaccination (author's transl)]. | A vaccination complication is only to be recognized if, taking into consideration the incubation time, the clinical picture coincides with that of spontaneous poliomyelitis. Apart from exceptional cases, virological studies are only of importance for the assessment if they are carried out in the acute or subacute stage... | [Vaccination complications after oral poliomyelitis vaccination (author's transl)]. A vaccination complication is only to be recognized if, taking into consideration the incubation time, the clinical picture coincides with that of spontaneous poliomyelitis. Apart from exceptional cases, virological studies are only of ... | [
-0.0017508553573861718,
-0.01592509262263775,
0.006201965268701315,
-0.05250146612524986,
-0.012947087176144123,
-0.01570729911327362,
0.011885827407240868,
0.05128937587141991,
-0.0502576120197773,
-0.02691522240638733,
-0.03936593607068062,
-0.07132728397846222,
0.07065839320421219,
-0.0... |
PMID:17067 | Chronic urticaria as a manifestation of necrotizing venulitis. | In a group of patients with a syndrome consisting of recurrent episodes of urticaria, arthralgia, abdominal pain, and (rarely) glomerulonephritis, examination of skin biopsy specimens showed necrotizing venulitis. An elevated erythrocyte sedimentation rate was the most common laboratory abnormality. Analyses of serum i... | Chronic urticaria as a manifestation of necrotizing venulitis. In a group of patients with a syndrome consisting of recurrent episodes of urticaria, arthralgia, abdominal pain, and (rarely) glomerulonephritis, examination of skin biopsy specimens showed necrotizing venulitis. An elevated erythrocyte sedimentation rate ... | [
-0.07035024464130402,
0.02366534247994423,
-0.0050039393827319145,
0.04444046691060066,
-0.004141862038522959,
-0.02917628176510334,
0.09117476642131805,
0.050208982080221176,
-0.011482248082756996,
0.0058892276138067245,
-0.004111992660909891,
-0.06903814524412155,
0.007982154376804829,
0... |
PMID:17069 | Isolation of Histoplasma capsulatum from soil in the Aguas Buenos Caves, Aguas Buenos, Puerto Rico. I. An ecological approach. | Only one out of 19 soil sampling sites chosen for isolation of H. capsulatum studies yielded positive results. The stations were located in Cueva Oscura, which is part of the Aguas Buenas Caves system. The habitat characteristics of these station are believed to be responsible for the presence of the fungus. | Isolation of Histoplasma capsulatum from soil in the Aguas Buenos Caves, Aguas Buenos, Puerto Rico. I. An ecological approach. Only one out of 19 soil sampling sites chosen for isolation of H. capsulatum studies yielded positive results. The stations were located in Cueva Oscura, which is part of the Aguas Buenas Caves... | [
0.03601984679698944,
-0.0501108318567276,
0.025798374786973,
-0.023673729971051216,
0.07278940081596375,
-0.014070181176066399,
-0.0012458701385185122,
-0.016395628452301025,
-0.04738523066043854,
0.03135460987687111,
0.022640665993094444,
-0.06267192959785461,
-0.030484430491924286,
0.038... |
PMID:17072 | X-ray study of the lithium complex of NAD. | The Li+-NAD+ complex exists as a 'dimer' of two molecules arranged head-to-tail with Li+ coordinated tetrahedrally to adenine N(7) and three pyrophosphate oxygens. Adenine is stacked intermolecularly on nicotinamide. The conformation of NAD+ is 'extended' and similar to that found in holoenzyme complexes. This is in co... | X-ray study of the lithium complex of NAD. The Li+-NAD+ complex exists as a 'dimer' of two molecules arranged head-to-tail with Li+ coordinated tetrahedrally to adenine N(7) and three pyrophosphate oxygens. Adenine is stacked intermolecularly on nicotinamide. The conformation of NAD+ is 'extended' and similar to that f... | [
-0.131944477558136,
0.01568049006164074,
-0.016577381640672684,
0.047217242419719696,
0.03650003299117088,
0.03586926683783531,
0.05604085326194763,
-0.06175106391310692,
-0.05322062969207764,
-0.029002554714679718,
0.004576093517243862,
-0.05529551953077316,
-0.04168042913079262,
0.029843... |
PMID:17084 | Effect of pyridoxine on the growth of Morris hepatoma No. 7288Ctc and enzyme activity. | The effect(s) of lack of dietary pyridoxine (PX) on the growth of Morris hepatoma no. 7288Ctc was studied. Buffalo strain female rats were fed a diet lacking PX. Pair-fed controls were fed the same diet with PX added. Animals were inoculated with no. 7288Ctc hepatoma cells at 21 days and were sacrificed 16 days later. ... | Effect of pyridoxine on the growth of Morris hepatoma No. 7288Ctc and enzyme activity. The effect(s) of lack of dietary pyridoxine (PX) on the growth of Morris hepatoma no. 7288Ctc was studied. Buffalo strain female rats were fed a diet lacking PX. Pair-fed controls were fed the same diet with PX added. Animals were in... | [
-0.014952505938708782,
0.03323393315076828,
0.018492432311177254,
0.01825389824807644,
-0.0036350637674331665,
-0.0012548016384243965,
0.037009064108133316,
0.05605839192867279,
-0.07483790069818497,
0.003985235467553139,
0.05730469897389412,
-0.07306848466396332,
0.02316862717270851,
0.02... |
PMID:17085 | Expression of hormonally induced tyrosine transaminase in normal, host liver and three Morris hepatomas. | Cytoplasmic tyrosine transaminase (L-tyrosine: 2-oxoglutarate aminotransferase, EC 2.6.1.5) was partially purified from normal, host liver and three Morris hepatomas and subsequently resolved by electrophoresis on polyacrylamide gels. Enzyme activity was detected histochemically in situ on the gels. Seven enzymatically... | Expression of hormonally induced tyrosine transaminase in normal, host liver and three Morris hepatomas. Cytoplasmic tyrosine transaminase (L-tyrosine: 2-oxoglutarate aminotransferase, EC 2.6.1.5) was partially purified from normal, host liver and three Morris hepatomas and subsequently resolved by electrophoresis on p... | [
-0.0011124671436846256,
0.012114250101149082,
-0.016714053228497505,
0.003429627511650324,
0.016606615856289864,
-0.036694906651973724,
0.09836601465940475,
0.04597901552915573,
-0.02511957287788391,
-0.008441809564828873,
0.06377460807561874,
-0.05455510690808296,
-0.0003053354739677161,
... |
PMID:17086 | [Allogenic inhibition of the stem hematopoietic cells in the bone marrow of adult mice and in the embryonal liver]. | The maternal effect was shown to influence the degree of allogenic inhibition of stem hemopoietic cells of the embryonic liver and adult bone marrow in CBA and C57Bl/6 mice. The display of allogenic inhibition of stem cells of the embryonic liver and adult bone marrow proved to be similar in C57Bl/6 mice and dissimilar... | [Allogenic inhibition of the stem hematopoietic cells in the bone marrow of adult mice and in the embryonal liver]. The maternal effect was shown to influence the degree of allogenic inhibition of stem hemopoietic cells of the embryonic liver and adult bone marrow in CBA and C57Bl/6 mice. The display of allogenic inhib... | [
-0.03271641209721565,
0.050710540264844894,
-0.01314057782292366,
-0.016599813476204872,
-0.043993186205625534,
0.009143415838479996,
0.03902970254421234,
0.08115019649267197,
-0.034121062606573105,
0.016665657982230186,
0.034898899495601654,
-0.027276352047920227,
-0.029310021549463272,
-... |
PMID:17087 | [Effect of syngenic lymphocytes on allogenic inhibition of hematopoietic stem cells of embryonic liver]. | The transplantation of liver from the embryos and newborn C57BL-6 mice to the lethally irradiated hybrids (CBA X C57BL/6) F1resulted in 90% allogenic inhibition of the colony-forming activity of the donor elements. The degree of allogenic inhibition of liver cells of 19 days old embryos and newborn mice may be changed ... | [Effect of syngenic lymphocytes on allogenic inhibition of hematopoietic stem cells of embryonic liver]. The transplantation of liver from the embryos and newborn C57BL-6 mice to the lethally irradiated hybrids (CBA X C57BL/6) F1resulted in 90% allogenic inhibition of the colony-forming activity of the donor elements. ... | [
-0.04724990576505661,
0.02988743968307972,
-0.027043232694268227,
0.01912129484117031,
-0.02946728840470314,
-0.028925566002726555,
0.055172864347696304,
0.1299460232257843,
-0.05052950605750084,
-0.012084363028407097,
0.05334313586354256,
-0.026228949427604675,
-0.01558679435402155,
-0.03... |
PMID:17088 | [NAD+/NADH ratio in the mitochondria of the oocytes and ova of groundling, Misgurnus fossilis (L.)]. | The ratio NAD+/NAD-H was detemined in mitochondria of the loach oocytes and eggs on the basis of concentrations of the glutamate dehydrogenase reaction intermediates. This ratio increases 6 times upon the oocyte maturation. The importance of this ratio in the metabolism of oocyte and embryo is discussed. | [NAD+/NADH ratio in the mitochondria of the oocytes and ova of groundling, Misgurnus fossilis (L.)]. The ratio NAD+/NAD-H was detemined in mitochondria of the loach oocytes and eggs on the basis of concentrations of the glutamate dehydrogenase reaction intermediates. This ratio increases 6 times upon the oocyte maturat... | [
0.0032553633209317923,
-0.031745344400405884,
-0.051086850464344025,
-0.012474382296204567,
0.06590044498443604,
0.02156241424381733,
0.04764746502041817,
-0.020092621445655823,
-0.007631994783878326,
-0.015873461961746216,
0.029379328712821007,
-0.12106706947088242,
0.04709358885884285,
0... |
PMID:17090 | A comprehensive treatment approach to chronic low back pain. | A comprehensive treatment program for chronic disability related to back disease has been presented. This program has used not only more traditional methods of medical care for the structural disabilities of chronic mechanical back disorders, but has used principles of active patient participation in the improvement pr... | A comprehensive treatment approach to chronic low back pain. A comprehensive treatment program for chronic disability related to back disease has been presented. This program has used not only more traditional methods of medical care for the structural disabilities of chronic mechanical back disorders, but has used pri... | [
-0.04317006096243858,
0.027526501566171646,
0.025438550859689713,
0.005457793362438679,
-0.012127118185162544,
-0.008027127012610435,
-0.005699704866856337,
0.05909581482410431,
-0.004395138937979937,
-0.06855360418558121,
0.03368622809648514,
-0.03256462141871452,
0.005610682535916567,
0.... |
PMID:17092 | Effect of valine on propionate metabolism in control and hyperglycinemic fibroblasts and in rat liver. | Measurement of methylmalonyl-CoA mutase and propionyl-CoA carboxylase activities in lysates from fibroblasts derived from control, nonketotic hyperglycinemia, propionic acidemia, and both vitamin B12-responsive and -nonresponsive variants of methylmalonic acidemia showed only one abnormality: a 59% decrease in carboxyl... | Effect of valine on propionate metabolism in control and hyperglycinemic fibroblasts and in rat liver. Measurement of methylmalonyl-CoA mutase and propionyl-CoA carboxylase activities in lysates from fibroblasts derived from control, nonketotic hyperglycinemia, propionic acidemia, and both vitamin B12-responsive and -n... | [
-0.030123015865683556,
0.003388773649930954,
-0.01077267900109291,
0.06931642442941666,
-0.012453915551304817,
-0.03615429252386093,
0.012050981633365154,
0.033221885561943054,
-0.11242865771055222,
-0.02681032568216324,
0.06456303596496582,
-0.04357841610908508,
0.0669742077589035,
0.0592... |
PMID:17093 | Otitis media in children less than 12 weeks of age. | Cases of otitis media in infants under 12 weeks of age were reviewed to delineate the frequency, clinical features, and etiologic agents involved. Tympanocentesis was performed in 42 infants, 0 to 5 weeks of age, and in 17, from 6 to 11 weeks of age. The most common symptoms were irritability/lethargy (69%), fever (52%... | Otitis media in children less than 12 weeks of age. Cases of otitis media in infants under 12 weeks of age were reviewed to delineate the frequency, clinical features, and etiologic agents involved. Tympanocentesis was performed in 42 infants, 0 to 5 weeks of age, and in 17, from 6 to 11 weeks of age. The most common s... | [
0.0043172286823391914,
-0.028645886108279228,
-0.008298461325466633,
-0.004033118486404419,
0.037447184324264526,
-0.018757035955786705,
0.0474541038274765,
0.05973941087722778,
-0.08355335146188736,
0.023259274661540985,
0.024495385587215424,
-0.04084843769669533,
-0.041774436831474304,
0... |
PMID:17095 | [Diagnosis of hepatic metastasis by enzymatic assay. Value of gamma-glutamyltranspeptidase]. | Variations in serum enzyme levels were studied in 50 patients suffering from various types of neoplasm, 25 of whom had hepatic metastases. In the absence of any associated hepato-biliary or pancreatic disorder, gamma-glutamyl-transpeptidase would appear to be a reliable test in 96 % of cases. It represents a factor of ... | [Diagnosis of hepatic metastasis by enzymatic assay. Value of gamma-glutamyltranspeptidase]. Variations in serum enzyme levels were studied in 50 patients suffering from various types of neoplasm, 25 of whom had hepatic metastases. In the absence of any associated hepato-biliary or pancreatic disorder, gamma-glutamyl-t... | [
-0.0021792282350361347,
-0.0512862391769886,
0.0015851063653826714,
-0.004743191879242659,
0.02777855470776558,
-0.0301051065325737,
0.10386519134044647,
0.06882106512784958,
-0.03751039505004883,
-0.03055439703166485,
0.017332781106233597,
-0.045187946408987045,
-0.021979890763759613,
0.0... |
PMID:17096 | Aminoacylation of tRNA Trp from beef liver, yeast and E. coli by beef pancrease tryptophan-tRNA ligase. Stoichiometry of tRNATrp binding. | The Michaelis constants and the maximum velocities in the aminoacylation reaction of tRNATrp from beef liver, yeast and E. coli by pure beef pancreas tryptophan-tRNA ligase show that this mammalian enzyme recognizes and charges the two eucaryotic tRNAs with the same efficiency. The rate of aminoacylation of the procary... | Aminoacylation of tRNA Trp from beef liver, yeast and E. coli by beef pancrease tryptophan-tRNA ligase. Stoichiometry of tRNATrp binding. The Michaelis constants and the maximum velocities in the aminoacylation reaction of tRNATrp from beef liver, yeast and E. coli by pure beef pancreas tryptophan-tRNA ligase show that... | [
-0.07015451788902283,
-0.043383385986089706,
-0.007894758135080338,
0.016553858295083046,
-0.032066795974969864,
-0.03708122670650482,
0.03638646751642227,
0.05779777467250824,
-0.017475532367825508,
0.002405647886916995,
-0.027302343398332596,
-0.07407725602388382,
-0.03899598494172096,
0... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.