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PMID:17308
Paradoxical response to dopamine agonists in tardive dyskinesia.
The authors conducted an extensive pharmacological analysis of a patient severely affected by tardive dyskinesia. No drug treatment gave lasting clinical improvement. Several agents recently recommended for this condition, dimethyl aminoethanol, clozapine, and thioridazine, failed to modify the dyskinesia. Reserpine ca...
Paradoxical response to dopamine agonists in tardive dyskinesia. The authors conducted an extensive pharmacological analysis of a patient severely affected by tardive dyskinesia. No drug treatment gave lasting clinical improvement. Several agents recently recommended for this condition, dimethyl aminoethanol, clozapine...
[ -0.08133624494075775, -0.014233850874006748, 0.040831588208675385, 0.09454509615898132, -0.06940381228923798, 0.001991847762838006, 0.05064278468489647, 0.03617009148001671, -0.02866230718791485, -0.053446196019649506, 0.005000689532607794, -0.013175837695598602, 0.024112876504659653, 0.09...
PMID:17310
Host feeding patterns of Connecticut mosquitoes (Diptera: Culicidae).
Blood-engorged Coquillettidia perturbans, Psorophora ferox, Culex, Culiseta, and Aedes mosquitoes were collected principally by sweep net from salt marsh and woodland habitats in Connecticut. Of the 570 mosquitoes tested, precipitin tests identified the origins of 517 blood meals and revealed distinct host feeding patt...
Host feeding patterns of Connecticut mosquitoes (Diptera: Culicidae). Blood-engorged Coquillettidia perturbans, Psorophora ferox, Culex, Culiseta, and Aedes mosquitoes were collected principally by sweep net from salt marsh and woodland habitats in Connecticut. Of the 570 mosquitoes tested, precipitin tests identified ...
[ 0.008992896415293217, -0.01626628078520298, -0.01528641302138567, 0.023902107030153275, 0.07648345082998276, 0.009468289092183113, 0.047910455614328384, 0.04291212186217308, -0.01876121386885643, 0.03129921481013298, -0.020201079547405243, -0.004647386725991964, -0.02449853904545307, 0.008...
PMID:17311
Malignant hypertension as a cause of massive intestinal bleeding.
A forty-one year old man with a three year history of malignant hypertension developed massive rectal bleeding. A mesenteric arteriogram showed vascular changes compatible with necrotizing angitis. Histologic examination of the resected right hemicolon confirmed malignant hypertension as the cause of angitis.
Malignant hypertension as a cause of massive intestinal bleeding. A forty-one year old man with a three year history of malignant hypertension developed massive rectal bleeding. A mesenteric arteriogram showed vascular changes compatible with necrotizing angitis. Histologic examination of the resected right hemicolon c...
[ -0.04062187671661377, -0.013919277116656303, 0.03522918000817299, 0.03790654242038727, 0.049643196165561676, -0.06697960942983627, 0.0927639901638031, 0.0317409485578537, -0.001875094952993095, -0.08550219237804413, 0.05283680930733681, -0.02138504944741726, 0.041016437113285065, 0.0119794...
PMID:17312
[Gerontology in rural and mountains regions aged people in the country and in mountain regions (author's transl)].
The gerontologic problems of people living in the country and in mountain regions always were neglected in comparison to those of townsmen. In the last decade an important structural change has happened, caused on the one side by the fact that more and more people leave the country for the towns, and by the problem of ...
[Gerontology in rural and mountains regions aged people in the country and in mountain regions (author's transl)]. The gerontologic problems of people living in the country and in mountain regions always were neglected in comparison to those of townsmen. In the last decade an important structural change has happened, c...
[ 0.003231193171814084, 0.022996384650468826, 0.02933020144701004, 0.043869491666555405, 0.0662451758980751, -0.0427539087831974, 0.0023338976316154003, -0.012960556894540787, -0.08731621503829956, -0.013556543737649918, 0.01428849995136261, -0.03025161102414131, 0.014424115419387817, 0.0071...
PMID:17313
[Problems of aged people in rural regions of the Swiss Canton St. Gall (author's transl)].
Based on the structure of the mountain population, the living conditions of the aged (in rural communities 11-17% are older than 65) are shown. By public planning and cooperation of private organizations a material security of the aged has been strived after and mostly reached in the last 15 years--with the tendency to...
[Problems of aged people in rural regions of the Swiss Canton St. Gall (author's transl)]. Based on the structure of the mountain population, the living conditions of the aged (in rural communities 11-17% are older than 65) are shown. By public planning and cooperation of private organizations a material security of th...
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PMID:17314
[Living conditions of the rural population in France. Situation in 1974 and evolution from 1967 to 1974 (author's transl)].
A survey of the living conditions of aged people in rural regions of France in 1974 and a comparison with the previous surveys of 1967/68 in 302 rural vallages are analysed. Of the 4,800 persons in 1967/68, by 1974 1,609 were dead (35%). After questioning the 2,191 living persons (1,275 farmers and 916 non farmers) an ...
[Living conditions of the rural population in France. Situation in 1974 and evolution from 1967 to 1974 (author's transl)]. A survey of the living conditions of aged people in rural regions of France in 1974 and a comparison with the previous surveys of 1967/68 in 302 rural vallages are analysed. Of the 4,800 persons i...
[ -0.017617888748645782, 0.027974022552371025, -0.050264544785022736, 0.0016515436582267284, 0.06764000654220581, 0.01867218315601349, 0.003835826413705945, 0.02742619998753071, -0.02626154199242592, -0.008757375180721283, 0.024281760677695274, -0.036945004016160965, 0.04074564576148987, -0....
PMID:17315
[Aging in cities and in the country (author's transl)].
A growing concern with ecological issues has led gerontologist to analyze the life-situation of aged people in cities and in the country more precisely. While it is generally assumed that aging in the country has advantages to aging in the city, statistical figures (e.g. income, living conditions, health status, educat...
[Aging in cities and in the country (author's transl)]. A growing concern with ecological issues has led gerontologist to analyze the life-situation of aged people in cities and in the country more precisely. While it is generally assumed that aging in the country has advantages to aging in the city, statistical figure...
[ 0.05678509175777435, 0.009469144977629185, -0.0032033699098974466, 0.00499678635969758, 0.060485515743494034, 0.005470403004437685, 0.025461377575993538, -0.007228992413729429, -0.046548496931791306, -0.020963463932275772, 0.021751008927822113, 0.029472943395376205, 0.014643755741417408, 0...
PMID:17316
[The old aged in a mountain community (author's transl)].
In a mountain community of Eastern Switzerland at 1000 m with about 1000 people more than 17% are older than 65. 2/3 of the aged live in their own household, 1/4 lives together with the young generation. Only 8% spend their late years in a home for the aged, which lodges also easy nursing cases. Cheaper flats, cooperat...
[The old aged in a mountain community (author's transl)]. In a mountain community of Eastern Switzerland at 1000 m with about 1000 people more than 17% are older than 65. 2/3 of the aged live in their own household, 1/4 lives together with the young generation. Only 8% spend their late years in a home for the aged, whi...
[ -0.007085030898451805, 0.012857562862336636, -0.028651336207985878, 0.034436941146850586, 0.04998951405286789, -0.01773955672979355, 0.021476423367857933, -0.03592867776751518, -0.026087980717420578, 0.008075688034296036, -0.03609858825802803, -0.025491146370768547, 0.005339949857443571, -...
PMID:17317
[Medical problems of the aged in a mountain community of the Swiss Canton Appenzell A.Rh. (author's transl)].
The report about a mountain community (Urnäsch) with 2400 inhabitants at 700-1100 m above sea level with 16-18% aged over 65 shows the help for the aged by several groups: women's club, church, cultural institutions. The community doctor looks after 4 homes for the aged. He also treats those patients living in their ow...
[Medical problems of the aged in a mountain community of the Swiss Canton Appenzell A.Rh. (author's transl)]. The report about a mountain community (Urnäsch) with 2400 inhabitants at 700-1100 m above sea level with 16-18% aged over 65 shows the help for the aged by several groups: women's club, church, cultural institu...
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PMID:17318
[The aged in a mountain region of the swiss grisons and their problems (author's transl)].
The way of life of the elderly people in a Swiss mountain valley (Schanfigg, Grisons) is statistically examined and illustrated by detailed descriptions. The author as general practitioner for the region (9 villages with a total of 1.500 persons, 20% over 65 years old) knows everybody personally. The majority of old pe...
[The aged in a mountain region of the swiss grisons and their problems (author's transl)]. The way of life of the elderly people in a Swiss mountain valley (Schanfigg, Grisons) is statistically examined and illustrated by detailed descriptions. The author as general practitioner for the region (9 villages with a total ...
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PMID:17321
Endogenous opiates-a progress report.
The "opiate receptor" has become a well-accepted entity, stimulating the search for an endogenous agonist. This has successfully revealed several different endogenous peptide opiates whose physiological role is under intense investigation, contributing to our understanding of pain and of opiate tolerance and dependenc...
Endogenous opiates-a progress report. The "opiate receptor" has become a well-accepted entity, stimulating the search for an endogenous agonist. This has successfully revealed several different endogenous peptide opiates whose physiological role is under intense investigation, contributing to our understanding of pain...
[ -0.006347973830997944, -0.03325515240430832, 0.015899788588285446, 0.015132171101868153, 0.007201063446700573, 0.0037457062862813473, 0.027063867077231407, 0.05694306269288063, -0.01802733913064003, 0.0034782462753355503, -0.014661267399787903, -0.010601836256682873, -0.000885025889147073, ...
PMID:17335
Effect of sodium bicarbonate on canine left ventricular function.
Left ventricular performance was evaluated in seven canine right heart bypass preparations during and after the intraauricular administration of sodium bicarbonate (100 ml, 1100 Osm, 60 mEq). Under conditions of constant cardiac input and left ventricular volume, sodium bicarbonate produced mild and brief left ventricu...
Effect of sodium bicarbonate on canine left ventricular function. Left ventricular performance was evaluated in seven canine right heart bypass preparations during and after the intraauricular administration of sodium bicarbonate (100 ml, 1100 Osm, 60 mEq). Under conditions of constant cardiac input and left ventricula...
[ -0.0678984746336937, -0.011245201341807842, 0.037989240139722824, 0.04984006658196449, -0.0377286933362484, 0.018696671351790428, 0.03353683650493622, 0.0281731728464365, -0.025481436401605606, -0.02234804630279541, 0.012407396920025349, -0.10190293937921524, -0.03365504741668701, 0.010779...
PMID:17340
Secretin-stimulated serum gastrin levels in hyperparathyroid patients from families with multiple endocrine adenomatosis type I.
Twenty-three patients with hyperparathyroidism from six families with the multiple endocrine adenomatosis (MEA) I-syndrome were tested by secretin provocation. In nine cases this led to increases in serum gastrin ranging from 298 to 13 300 pg/ml, whereas the maximum rise in gastrin in the other 14 patients was 32 pg/ml...
Secretin-stimulated serum gastrin levels in hyperparathyroid patients from families with multiple endocrine adenomatosis type I. Twenty-three patients with hyperparathyroidism from six families with the multiple endocrine adenomatosis (MEA) I-syndrome were tested by secretin provocation. In nine cases this led to incre...
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PMID:17344
[Cecal motility in the rabbit. III. Duality of fecal excretion].
The production of soft pellets (caecotrophs) was studied in conjunction with feeding behaviour, biochemical changes in caecal contents, motility of the caeco-colic region and transit of digesta in the colon in rabbits fed ad lib. or receiving one or two daily meals. Studies were carried out on animals either collared o...
[Cecal motility in the rabbit. III. Duality of fecal excretion]. The production of soft pellets (caecotrophs) was studied in conjunction with feeding behaviour, biochemical changes in caecal contents, motility of the caeco-colic region and transit of digesta in the colon in rabbits fed ad lib. or receiving one or two d...
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PMID:17347
Experiments for the production of graft-versus-host reaction in adult and infantile mice by means of human immunocompetent cells.
By administering anti-mouse ATS five times to random-bred Swiss albino mice weighing 16-18 and 10-11 g respectively, the authors have tried to produce immunological depression of such a degree which would allow living human leukocytes to cause GVH reaction. On the basis of the results it may be stated that with the ser...
Experiments for the production of graft-versus-host reaction in adult and infantile mice by means of human immunocompetent cells. By administering anti-mouse ATS five times to random-bred Swiss albino mice weighing 16-18 and 10-11 g respectively, the authors have tried to produce immunological depression of such a degr...
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PMID:17348
Exeriments for the production of graft-versus-host reaction in newborn mice by means of human immunocompetent cells.
It was not possible to produce the wasting disease in newborn mice by administering human leukocytes in Hanks' solution, or suspended in m-ATS or h-ATS. The attempt of the authors to produce immunosuppression by the administration of m-ATS started at newborn age-as a result of which the human immunocompetent cells woul...
Exeriments for the production of graft-versus-host reaction in newborn mice by means of human immunocompetent cells. It was not possible to produce the wasting disease in newborn mice by administering human leukocytes in Hanks' solution, or suspended in m-ATS or h-ATS. The attempt of the authors to produce immunosuppre...
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PMID:17349
Study on the interaction of lymphotropic cytostatic agent treatment and graft-versus-host (GVH) reaction in mice.
F1 hybrid mice (C57BL X C3H) suffering from graft-versus-host disease, were treated with dianhydrodulcit (DAD) a lymphotrop cytostatic agent. 15 mg/kg of the compound was injected intraperitoneally, 8 days following the administration of the parental spleen cell suspension. The interaction between the two interventions...
Study on the interaction of lymphotropic cytostatic agent treatment and graft-versus-host (GVH) reaction in mice. F1 hybrid mice (C57BL X C3H) suffering from graft-versus-host disease, were treated with dianhydrodulcit (DAD) a lymphotrop cytostatic agent. 15 mg/kg of the compound was injected intraperitoneally, 8 days ...
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PMID:17364
Oxidation of stibnite by Thiobacillus ferrooxidans.
Optimum pH, temperature and pulp density for microbiological leaching of museum-grade stibnite mineral has been investigated using a stibnite-adapted strain of Thiobacillus ferrooxidans. Optimum conditions were found to be pH 1.75, 35 C and 12g solid substrate per 100 ml of basal salts medium as the initial dose. The ...
Oxidation of stibnite by Thiobacillus ferrooxidans. Optimum pH, temperature and pulp density for microbiological leaching of museum-grade stibnite mineral has been investigated using a stibnite-adapted strain of Thiobacillus ferrooxidans. Optimum conditions were found to be pH 1.75, 35 C and 12g solid substrate per 10...
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PMID:17365
Fungal growth on C1 compounds: quantitative aspects of growth of a methanol-utilizing strain of Trichoderma lignorum in batch culture.
A study was made of some salient parameters that influence growth of the methanol-utilizing fungus Trichoderma lignorum growing in batch culture on a minimal medium containing methanol as the sole source of carbon. Maximum cell yield was recorded at the expense of 1.58 g of methanol per liter. Inhibition was observed w...
Fungal growth on C1 compounds: quantitative aspects of growth of a methanol-utilizing strain of Trichoderma lignorum in batch culture. A study was made of some salient parameters that influence growth of the methanol-utilizing fungus Trichoderma lignorum growing in batch culture on a minimal medium containing methanol ...
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PMID:17366
Iodination of staphylococcal enterotoxin B by use of chloramine-T.
This report describes the conditions that are necessary for iodination of staphylococcal enterotoxin B (SEB) by use of chloramine-T. Makor Chemical Co. SEB and the two major SEB components, which were prepared by isoelectric focusing of partially purified SEB, were used in these studies. The antigenic activity of the S...
Iodination of staphylococcal enterotoxin B by use of chloramine-T. This report describes the conditions that are necessary for iodination of staphylococcal enterotoxin B (SEB) by use of chloramine-T. Makor Chemical Co. SEB and the two major SEB components, which were prepared by isoelectric focusing of partially purifi...
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PMID:17367
Identification of the virucidal agent in wastewater sludge.
Anaerobically digested sludge contains an agent that causes irreversible inactivation of poliovirus. It has now been shown that the agent responsible for this activity is ammonia. The effect of ammonia on poliovirus appears to be typical for picornaviruses, but reovirus, an enteric virus of another group, is quite resi...
Identification of the virucidal agent in wastewater sludge. Anaerobically digested sludge contains an agent that causes irreversible inactivation of poliovirus. It has now been shown that the agent responsible for this activity is ammonia. The effect of ammonia on poliovirus appears to be typical for picornaviruses, bu...
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PMID:17368
Graft-vs-host reaction.
Observations surrounding the clinical manifestations and pathological studies of a neonate who died at 9 weeks of age, indicate that distinctive cutaneous, histopathologic, and ultrastructural findings occur when graft-vs-host reaction (GVHR) complicates combined immune deficiency syndrome (CIDS). The prominence and sp...
Graft-vs-host reaction. Observations surrounding the clinical manifestations and pathological studies of a neonate who died at 9 weeks of age, indicate that distinctive cutaneous, histopathologic, and ultrastructural findings occur when graft-vs-host reaction (GVHR) complicates combined immune deficiency syndrome (CIDS...
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PMID:17370
Haemagglutination tests in the study of Ascaris epidemiology.
Haemagglutination tests using adult Ascaris suum antigen were performed on sera from 810 people from three areas in Papua New Guinea and East Timor. In the area with the highest childhood Ascaris infection rates and egg-counts (Kaul), titres were highest and remained high throughout life. There was a significant negati...
Haemagglutination tests in the study of Ascaris epidemiology. Haemagglutination tests using adult Ascaris suum antigen were performed on sera from 810 people from three areas in Papua New Guinea and East Timor. In the area with the highest childhood Ascaris infection rates and egg-counts (Kaul), titres were highest and...
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PMID:17371
Effects of various neuroleptics on rabbit hyperthermia induced by N, N-Dimethyltryptamine (DMT) and d-amphetamine.
The effects of various neuroleptics were studied on N, N-dimethyltryptamine (DMT, 3.2 mg/kg) and d-amphetamine (3.2 mg/kg) induced hyperthermia in the rabbit. Complete dose-effect curves were obtained. The order of potency for antagonism of DMT-induced hyperthermia was: methiothepin greater than octoclothepin greater t...
Effects of various neuroleptics on rabbit hyperthermia induced by N, N-Dimethyltryptamine (DMT) and d-amphetamine. The effects of various neuroleptics were studied on N, N-dimethyltryptamine (DMT, 3.2 mg/kg) and d-amphetamine (3.2 mg/kg) induced hyperthermia in the rabbit. Complete dose-effect curves were obtained. The...
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PMID:17374
Inhibition of dopamine synthesis in chronic schizophrenia. Clinical ineffectiveness of metyrosine.
According to the dopamine (DA) hypothesis of schizophrenia, there is a functional excess of dopaminergic activity within unspecified areas of the brain in schizophrenic patients. As a clinical test of this hypothesis, we administered metyrosine for three weeks to symptomatic chronic male schizophrenic patients who were...
Inhibition of dopamine synthesis in chronic schizophrenia. Clinical ineffectiveness of metyrosine. According to the dopamine (DA) hypothesis of schizophrenia, there is a functional excess of dopaminergic activity within unspecified areas of the brain in schizophrenic patients. As a clinical test of this hypothesis, we ...
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PMID:17375
Blood serum peptidases in patients with ovarian carcinoma treated with Ledakrin.
The authors observed changes in activity of leucine amino-peptidase (E.C. 3.4.1.1--LAP), gamma-glutamyl transpeptidase (F.C 2.3.2.2--GGTP) and Co++-activated acylase in blood serum of patients with ovarian carcinoma, with or without ascites, treated with Ledakrin (1-nitro-9-[3-dimethylaminopropylamino] acridine). It wa...
Blood serum peptidases in patients with ovarian carcinoma treated with Ledakrin. The authors observed changes in activity of leucine amino-peptidase (E.C. 3.4.1.1--LAP), gamma-glutamyl transpeptidase (F.C 2.3.2.2--GGTP) and Co++-activated acylase in blood serum of patients with ovarian carcinoma, with or without ascite...
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PMID:17376
[Ways of correcting certain metabolic processes in the myocardium in myocardial insufficiency].
The article deals with the following trends along which according to the author, investigations aimed at search for ways of correcting disturbed metabolic processes in the cardiac muscle in its incompetence should develop: 1) restoration of properties of contractile proteins and creation of a basis for successful forma...
[Ways of correcting certain metabolic processes in the myocardium in myocardial insufficiency]. The article deals with the following trends along which according to the author, investigations aimed at search for ways of correcting disturbed metabolic processes in the cardiac muscle in its incompetence should develop: 1...
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PMID:17377
[Use of formaldehyde with pH 8 0 as a fixative for electron and fluorescence microscopy].
For fixation of the material to be investigated at the light and ultrastuctural level the author suggests to use 4% formaldehyde obtained by depolymerization of paraform on 0.1 M phosphate buffer with end pH 8.0 of the solution. This fixative stabilizes well glycogen and tissue structure. The ultrastructural characteri...
[Use of formaldehyde with pH 8 0 as a fixative for electron and fluorescence microscopy]. For fixation of the material to be investigated at the light and ultrastuctural level the author suggests to use 4% formaldehyde obtained by depolymerization of paraform on 0.1 M phosphate buffer with end pH 8.0 of the solution. T...
[ 0.0005797924823127687, 0.014928223565220833, 0.02242887020111084, 0.007648529950529337, 0.010556823574006557, -0.03802283853292465, -0.008078150451183319, 0.007802083157002926, -0.05900922790169716, 0.0005908312741667032, 0.012681324034929276, -0.05149831995368004, -0.02173895202577114, 0....
PMID:17378
Hydrogen ion concentration of human tears: Effects of prolonged eye closure.
Using a closed chamber microelectrode technique, human tear hydrogen ion concentration was measured following periods of eye closure of 6 to 8 hours and then compared with normal open eye findings from the same patients. Closed eye tears were found to be significantly more acid (average age difference = 26 nM/1) and re...
Hydrogen ion concentration of human tears: Effects of prolonged eye closure. Using a closed chamber microelectrode technique, human tear hydrogen ion concentration was measured following periods of eye closure of 6 to 8 hours and then compared with normal open eye findings from the same patients. Closed eye tears were ...
[ -0.007605979219079018, 0.0034972657449543476, 0.029613085091114044, 0.03910709545016289, 0.0038679572753608227, 0.017450924962759018, 0.06604837626218796, 0.026014136150479317, -0.03459645062685013, -0.052828431129455566, 0.01776663400232792, -0.0801793709397316, -0.02311747893691063, 0.05...
PMID:17380
Surgery for unstable angina.
A series of 84 patients with unstable angina, treated surgically by grafting procedures between October 1970 and September 1976, have been reviewed. The study indicates that extensive coronary artery disease is common in these patients, and suggests that operation may favourably influence mortality, both immediate and ...
Surgery for unstable angina. A series of 84 patients with unstable angina, treated surgically by grafting procedures between October 1970 and September 1976, have been reviewed. The study indicates that extensive coronary artery disease is common in these patients, and suggests that operation may favourably influence m...
[ 0.02442343905568123, -0.004597429186105728, 0.0163438618183136, 0.06736349314451218, -0.020078878849744797, -0.009493140503764153, 0.04743668809533119, 0.02497430518269539, -0.0009454934624955058, 0.016526345163583755, -0.01628243550658226, -0.06631197780370712, -0.009282450191676617, 0.02...
PMID:17381
Rapid inpatient treatment of severe female sexual dysfunction.
A mult-faceted inpatient programme for overcoming severe female sexual of dysfunction using behavior modification techniques is described. The results obtained in a pilot study on 17 consecutive patients are presenre present.
Rapid inpatient treatment of severe female sexual dysfunction. A mult-faceted inpatient programme for overcoming severe female sexual of dysfunction using behavior modification techniques is described. The results obtained in a pilot study on 17 consecutive patients are presenre present.
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PMID:17386
Properties of glutathione release observed during reduction of organic hydroperoxide, demethylation of aminopyrine and oxidation of some substances in perfused rat liver, and their implications for the physiological function of catalase.
The enhanced reduction of t-butyl hydroperoxide by glutathione peroxidase is accompanied by a decrease in the cellular concentration of both glutathione and NADPH in isolated liver cells, resulting in the release of GSSG (oxidized glutathione) from the perfused rat liver. This phenomenon, first reported by H. Sies, C. ...
Properties of glutathione release observed during reduction of organic hydroperoxide, demethylation of aminopyrine and oxidation of some substances in perfused rat liver, and their implications for the physiological function of catalase. The enhanced reduction of t-butyl hydroperoxide by glutathione peroxidase is accom...
[ -0.013779711909592152, 0.007004601880908012, 0.005089309997856617, -0.006771635264158249, 0.04709560424089432, -0.015723107382655144, 0.04155724495649338, -0.00018443205044604838, -0.04519042372703552, -0.022591670975089073, -0.030085617676377296, -0.014783876948058605, 0.020878968760371208,...
PMID:17387
A kinetic study of the soluble 5'-nucleotidase of rat liver.
1. The kinetic properties of the 5'-nucleotidase (EC 3.1.3.5) present in the cytosol of rat liver were investigated in relation to the conversion of adenine nucleotides into uric acid, with particular reference to the stimulation of this process by fructose. The enzyme was assayed by the release of Pi and by a new and ...
A kinetic study of the soluble 5'-nucleotidase of rat liver. 1. The kinetic properties of the 5'-nucleotidase (EC 3.1.3.5) present in the cytosol of rat liver were investigated in relation to the conversion of adenine nucleotides into uric acid, with particular reference to the stimulation of this process by fructose. ...
[ -0.0686323344707489, -0.023287523537874222, -0.0048900181427598, -0.018490374088287354, 0.03137795254588127, 0.02062162384390831, 0.05095789581537247, 0.04836038872599602, -0.041840288788080215, 0.01115010678768158, 0.08665633201599121, -0.05104872211813927, -0.03650761395692825, -0.032104...
PMID:17388
Changes in mammary-gland acetyl-coenzyme A carboxylase associated with lactogenic differentiation.
The process leading to the rise of acetyl-CoA carboxylase activity in rat mammary tissue after the onset of lactation was investigated. The kinetics of change in enzyme activity and enzyme immunotitratable with antibody against avian liver acetyl-CoA carboxylase were determined during the course of lactogenic different...
Changes in mammary-gland acetyl-coenzyme A carboxylase associated with lactogenic differentiation. The process leading to the rise of acetyl-CoA carboxylase activity in rat mammary tissue after the onset of lactation was investigated. The kinetics of change in enzyme activity and enzyme immunotitratable with antibody a...
[ -0.07212144136428833, -0.0572761669754982, 0.01426562201231718, 0.039257921278476715, 0.0012739987578243017, 0.007343510631471872, 0.07258656620979309, 0.05453687533736229, -0.032043714076280594, -0.052225448191165924, 0.039010923355817795, -0.0628402903676033, -0.014859416522085667, 0.003...
PMID:17389
Possible multiple binding sites for o-aminophenol on uridine diphosphate glucuronyltransferase.
1. Hepatic microsomal UDP-glucuronyltransferase (EC 2.4.1.17) derived from either weanling or adult rats exhibits three pH optima, at pH 5.4, 7.2 and 9.2, when o-aminophenol is the acceptor substrate, whereas p-nitrophenol is the acceptor substrate only on pH optimum is observed, at pH 5.4.2. Prior treatment of rats of...
Possible multiple binding sites for o-aminophenol on uridine diphosphate glucuronyltransferase. 1. Hepatic microsomal UDP-glucuronyltransferase (EC 2.4.1.17) derived from either weanling or adult rats exhibits three pH optima, at pH 5.4, 7.2 and 9.2, when o-aminophenol is the acceptor substrate, whereas p-nitrophenol i...
[ -0.04669583588838577, -0.03250313177704811, -0.025707542896270752, 0.026301387697458267, 0.025854770094156265, 0.017035655677318573, 0.005279200617223978, 0.020018693059682846, -0.0196699146181345, -0.013232545927166939, 0.04919462278485298, -0.09764960408210754, 0.004038850776851177, 0.04...
PMID:17390
Purification of two hexosaminidases from human kidney.
Hexosaminidase forms A and B were isolated from human kidney in a homogeneous state as demonstrated by electrophoretic and enzymic criteria. The enzymes were stable for at least 18 months when stored at -20 degrees C in 0.025 M-phosphate buffer, pH 6.5. The molecular weights of forms A and B were estimated by gel filtr...
Purification of two hexosaminidases from human kidney. Hexosaminidase forms A and B were isolated from human kidney in a homogeneous state as demonstrated by electrophoretic and enzymic criteria. The enzymes were stable for at least 18 months when stored at -20 degrees C in 0.025 M-phosphate buffer, pH 6.5. The molecul...
[ -0.060818593949079514, -0.017412850633263588, 0.029560543596744537, -0.034690581262111664, -0.0014015368651598692, -0.03177298232913017, 0.04145370423793793, 0.0628989115357399, -0.06354117393493652, -0.02766036055982113, -0.007148581091314554, -0.06000445783138275, 0.001036625704728067, 0...
PMID:17391
The kinetics of the recombination reaction between apomyoglobin and alkaline haematin.
Apomyoglobin was prepared by an extremely mild modification of the acid/butanone technique, and the kinetics of the recombination reaction between this preparation and alkaline haematin were studied. The recombination has been shown to be precisely second-order and mono-phasic. Rate constants obtained from the study ar...
The kinetics of the recombination reaction between apomyoglobin and alkaline haematin. Apomyoglobin was prepared by an extremely mild modification of the acid/butanone technique, and the kinetics of the recombination reaction between this preparation and alkaline haematin were studied. The recombination has been shown ...
[ -0.09800361096858978, 0.06296858936548233, -0.015599747188389301, -0.017428532242774963, -0.028000477701425552, -0.05415869876742363, 0.04055967554450035, 0.03050866723060608, -0.02670763060450554, 0.026879338547587395, 0.026421567425131798, -0.05675838887691498, 0.0028499464970082045, 0.0...
PMID:17392
Assay of flavin nucleotides in pancreatic islets by a differential fluorimetric technique.
Differences in fluorescence intensity between FMN and FAD at high and low pH values were utilized to determine the concentration of each nucleotide. Amounts down to 1 pmol were measurable. The FMN and FAD contents of pancreatic islets of obese-hyperglycaemic mice (gene symbol ob/ob) were compared with those of the exoc...
Assay of flavin nucleotides in pancreatic islets by a differential fluorimetric technique. Differences in fluorescence intensity between FMN and FAD at high and low pH values were utilized to determine the concentration of each nucleotide. Amounts down to 1 pmol were measurable. The FMN and FAD contents of pancreatic i...
[ -0.020526885986328125, -0.007911600172519684, -0.013220476917922497, -0.015191550366580486, 0.017933929339051247, 0.011022829450666904, 0.07558175921440125, 0.0685998797416687, -0.026454772800207138, -0.06720317900180817, -0.020746126770973206, -0.08102675527334213, -0.03512601926922798, -...
PMID:17393
The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its application as a protein-labelling reagent.
4-Chloro-7-nitrobenzofurazan possesses at least one highly electrophilic centre (C-6) that is much more reactive towards nucleophiles than position C-4, the irreversible alkylating site of this reagent. Possible consequences of the electrophilic character of 4-chloro-7-nitrobenzofurazan for its application as a protein...
The highly electrophilic character of 4-chloro-7-nitrobenzofurazan and possible consequences for its application as a protein-labelling reagent. 4-Chloro-7-nitrobenzofurazan possesses at least one highly electrophilic centre (C-6) that is much more reactive towards nucleophiles than position C-4, the irreversible alkyl...
[ -0.04964885860681534, -0.015277503058314323, -0.06732423603534698, -0.03246862441301346, 0.012310672551393509, 0.025466157123446465, 0.05161205679178238, 0.04676179215312004, -0.08704856038093567, -0.0034878295846283436, -0.015801113098859787, 0.00018817292584571987, 0.008351286873221397, ...
PMID:17394
Acid dissociation constants of glycopeptides.
Glycopeptides containing mainly four amino acid residues in the sequence Asn-Leu-Thr-Ser, with small amounts of additional amino acid residues, were isolated from enzymic hydrolysates of hen's-egg albumin. Heterogeneity of the carbohydrate moiety was confirmed. Acid-base titrations showed that the alpha-amino group has...
Acid dissociation constants of glycopeptides. Glycopeptides containing mainly four amino acid residues in the sequence Asn-Leu-Thr-Ser, with small amounts of additional amino acid residues, were isolated from enzymic hydrolysates of hen's-egg albumin. Heterogeneity of the carbohydrate moiety was confirmed. Acid-base ti...
[ -0.06178492680191994, -0.05437719449400902, -0.05762816220521927, -0.007767709903419018, 0.019908268004655838, -0.010065043345093727, 0.044272612780332565, -0.013573329895734787, -0.017190566286444664, 0.01245925948023796, 0.06543984264135361, -0.04533294588327408, 0.03961179032921791, 0.0...
PMID:17395
The apparent absence of involvement of biotin in the vitamin K-dependent carboxylation of glutamic acid residues of proteins.
The mechanism of the vitamin K-dependent post-translational carboxylation of the gamma-carbon atom of glutamic acid residues in proteins remains obscure. Experiments were performed in vivo and in vitro in an attempt to establish a role for biotin in the transfer of the carboxyl group. Weanling male rats were fed on a b...
The apparent absence of involvement of biotin in the vitamin K-dependent carboxylation of glutamic acid residues of proteins. The mechanism of the vitamin K-dependent post-translational carboxylation of the gamma-carbon atom of glutamic acid residues in proteins remains obscure. Experiments were performed in vivo and i...
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PMID:17396
The binding of calcium to a salivary phosphoprotein, protein C, and comparison with calcium binding to protein A, a related salivary phosphoprotein.
The binding of Ca2+ to a salivary phosphoprotein, protein C, was studied by equilibrium dialysis. In 5mM-Tris/HCl buffer, pH 7.5, protein C bound 190 nmol of Ca2+/mg of protein. The apparent dissociation constant, K, was determined to be 1.9 x 10(-4)M and the binding of Ca2+ to the protein was non-co-operative. The bin...
The binding of calcium to a salivary phosphoprotein, protein C, and comparison with calcium binding to protein A, a related salivary phosphoprotein. The binding of Ca2+ to a salivary phosphoprotein, protein C, was studied by equilibrium dialysis. In 5mM-Tris/HCl buffer, pH 7.5, protein C bound 190 nmol of Ca2+/mg of pr...
[ -0.07335987687110901, -0.001657074666582048, -0.008857636712491512, -0.0006633311277255416, -0.03946341574192047, -0.06942124664783478, 0.10559304803609848, 0.03256535157561302, -0.00858904980123043, 0.0023144930601119995, 0.02265985496342182, -0.13443909585475922, 0.0005472130142152309, 0...
PMID:17397
The interaction of N-acetylhexosaminidase with insolubilized concanavalin A.
The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. Elution of the enzyme from insolubilized concanavalin A is dependent on both te...
The interaction of N-acetylhexosaminidase with insolubilized concanavalin A. The specific interaction between human N-acetylhexosaminidase and concanavalin A was evaluated with respect to temperature, time, pH and concentration of specific ligand in incubation mixtures containing the enzyme and insolubilized lectin. El...
[ -0.10724381357431412, -0.03159162402153015, -0.04157303646206856, -0.03333074599504471, -0.008054147474467754, -0.07140570878982544, 0.06952726095914841, 0.03973620757460594, -0.0652017593383789, -0.0492771714925766, 0.012449719943106174, -0.06290706992149353, -0.007824521511793137, 0.0256...
PMID:17398
Purification of carboxypeptidase B from human pancreas.
Carboxypeptidase B of the human pancreas was purified by chromatography on DEAE-cellulose and CM-cellulose columns. Two forms of the enzyme, named carboxypeptidase B1 and B2, were separated. They have similar mol.wts. (34250 +/- 590) as established by polyacrylamide-gel disc electrophoresis and by gel filtration. Carbo...
Purification of carboxypeptidase B from human pancreas. Carboxypeptidase B of the human pancreas was purified by chromatography on DEAE-cellulose and CM-cellulose columns. Two forms of the enzyme, named carboxypeptidase B1 and B2, were separated. They have similar mol.wts. (34250 +/- 590) as established by polyacrylami...
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PMID:17399
[19F]fluorine nuclear-magnetic-resonance study of the interaction of difluoro-oxaloacetate with aspartate transaminase.
Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant of which has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the pr...
[19F]fluorine nuclear-magnetic-resonance study of the interaction of difluoro-oxaloacetate with aspartate transaminase. Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant of which has been determined spectrophotometrically and by 19F n.m.r. (nuc...
[ -0.1017083004117012, 0.02380959875881672, -0.04750429093837738, 0.033342473208904266, 0.015911046415567398, -0.02358655072748661, 0.0648958832025528, 0.00977652333676815, -0.04047528654336929, -0.07320622354745865, 0.014758238568902016, -0.05991789326071739, 0.01873713545501232, 0.03716753...
PMID:17400
The chemistry of the collagen cross-links. Characterization of the products of reduction of skin, tendon and bone with sodium cyanoborohydride.
Reduction of tissues with sodium cyanoborohydride at pH7.4 gave results identical with those obtained by KBH4 treatment. On reduction with sodium cyanoborohydride at pH 4.4, however, a previously undetected basic compound was formed and was identified by mass spectrometry and chemical degradation techniques as dihydroh...
The chemistry of the collagen cross-links. Characterization of the products of reduction of skin, tendon and bone with sodium cyanoborohydride. Reduction of tissues with sodium cyanoborohydride at pH7.4 gave results identical with those obtained by KBH4 treatment. On reduction with sodium cyanoborohydride at pH 4.4, ho...
[ -0.08858059346675873, -0.021893583238124847, 0.013700047507882118, 0.06633447110652924, 0.000994964619167149, -0.01999821327626705, 0.01866721734404564, 0.028982114046812057, -0.07224056124687195, -0.010267318226397038, 0.03677725791931152, -0.06407743692398071, 0.018039662390947342, 0.025...
PMID:17401
Characterization of microsomal choloyl-coenzyme A synthetase.
Choloyl-CoA synthetase (EC 6.2.1.7) was characterized for the first time under appropriated assay conditions. The p/ optimum for the reaction is pH 7.2.-7.3. The reaction has an absolute requirement for bivalent cation. Several different metal ions fulfil this requirement, but Mn2+ and Mg2+ were the most effective. The...
Characterization of microsomal choloyl-coenzyme A synthetase. Choloyl-CoA synthetase (EC 6.2.1.7) was characterized for the first time under appropriated assay conditions. The p/ optimum for the reaction is pH 7.2.-7.3. The reaction has an absolute requirement for bivalent cation. Several different metal ions fulfil th...
[ -0.07504779100418091, -0.023823263123631477, -0.029076263308525085, 0.02988932654261589, -0.03377673402428627, -0.027616726234555244, 0.021122237667441368, 0.0058984290808439255, -0.058931782841682434, -0.04021565616130829, 0.05305165797472, -0.09204870462417603, -0.0032256843987852335, -0...
PMID:17411
Chronic nondestructive arthritis associated with cutaneous polyarteritis.
Two patients with arthritis of the knee joints associated with cutaneous polyarteritis have been followed for 20 and 5 years. The arthritis is characterized by mild to moderate pain and stiffness and inflammatory joint effusions with predominantly polymorphonuclear leukocytes. Despite its chronicity, there has been no ...
Chronic nondestructive arthritis associated with cutaneous polyarteritis. Two patients with arthritis of the knee joints associated with cutaneous polyarteritis have been followed for 20 and 5 years. The arthritis is characterized by mild to moderate pain and stiffness and inflammatory joint effusions with predominantl...
[ -0.061036426573991776, 0.005443598609417677, 0.03190840780735016, 0.050457362085580826, -0.004807547200471163, -0.01782332733273506, 0.045988958328962326, 0.07606473565101624, -0.016665736213326454, -0.030926771461963654, 0.031536683440208435, 0.012454073876142502, 0.03174944221973419, 0.0...
PMID:17412
Arthritis rounds. Pneumocystis carinii associated with polyarteritis and immunosuppressive therapy.
Pneumocystis carinii characteristically causes pneumonia in patients with immunodeficiency disorders. It occurs most often in patients with malignancy or renal transplants whose immune response has been suppressed by corticosteroids or cytotoxic agents. Individuals with connective tissue disease who receive immunosuppr...
Arthritis rounds. Pneumocystis carinii associated with polyarteritis and immunosuppressive therapy. Pneumocystis carinii characteristically causes pneumonia in patients with immunodeficiency disorders. It occurs most often in patients with malignancy or renal transplants whose immune response has been suppressed by cor...
[ -0.04297475516796112, -0.025548921898007393, -0.012764450162649155, 0.020767588168382645, -0.021555818617343903, -0.04546336084604263, 0.05533991754055023, 0.048721566796302795, -0.07563202828168869, -0.018737848848104477, 0.006785540841519833, -0.004425459541380405, 0.042310431599617004, ...
PMID:17413
[Influence of antirheumatic phenylacetic acid derivatives on glycosaminoglycan metabolism of fibroblast culture (author's transl)].
Murine embryonic fibroblast monolayer cultures were used to study the influence of the antirheumatic phenylacetic acid derivatives ibufanac, ibuprofen, alclofenac and bufexamac and of phenylbutazone as a reference compound on glycosaminoglycan (GAG) metabolism viability and multiplication of cells cultured in vitro. Th...
[Influence of antirheumatic phenylacetic acid derivatives on glycosaminoglycan metabolism of fibroblast culture (author's transl)]. Murine embryonic fibroblast monolayer cultures were used to study the influence of the antirheumatic phenylacetic acid derivatives ibufanac, ibuprofen, alclofenac and bufexamac and of phen...
[ -0.023959437385201454, -0.0243607796728611, 0.00823003426194191, 0.009164450690150261, 0.035297561436891556, -0.02785748615860939, -0.01167997531592846, 0.039517104625701904, -0.10227487981319427, -0.07024719566106796, 0.06390688568353653, -0.01288994774222374, 0.05324704945087433, 0.01415...
PMID:17414
[Treatment of coronary insufficiency with beta-receptor antagonists (author's transl)].
The pathophysiology of the coronary insufficiency, the effect of beta-receptor antagonists, the understanding of these effects on the basis of the pathophysiology and the possibilities of testing beta-receptor antagonists in patients with coronary insufficiency are discussed. It is pointed out that the pathophysiology ...
[Treatment of coronary insufficiency with beta-receptor antagonists (author's transl)]. The pathophysiology of the coronary insufficiency, the effect of beta-receptor antagonists, the understanding of these effects on the basis of the pathophysiology and the possibilities of testing beta-receptor antagonists in patient...
[ -0.036699943244457245, 0.017117369920015335, -0.0013357496354728937, 0.0998668298125267, 0.018068434670567513, -0.035373616963624954, 0.025568000972270966, 0.009358735755085945, -0.026083946228027344, -0.03714956343173981, -0.016699889674782753, -0.058946818113327026, -0.006171314977109432, ...
PMID:17416
[Quantitative parameters of sympatho-neuronal and sympatho-adrenal activities in man. The influence of beta-receptor blocking agents (author's transl)].
Determination of both catecholamine concentrations (norepinephrine and epinephrine) and the activity of dopamine-beta-hydroxylase in the plasma of health volunteers and hypertensive patients leads to the conclusion that these parameters used together can be considered an index for sympathetic neuronal activity. However...
[Quantitative parameters of sympatho-neuronal and sympatho-adrenal activities in man. The influence of beta-receptor blocking agents (author's transl)]. Determination of both catecholamine concentrations (norepinephrine and epinephrine) and the activity of dopamine-beta-hydroxylase in the plasma of health volunteers an...
[ -0.02185753546655178, 0.022609606385231018, 0.010900178924202919, 0.027951041236519814, -0.0012020788853988051, 0.01126199308782816, 0.06905142962932587, -0.0028167839627712965, -0.023484984412789345, -0.0796002596616745, -0.03242054209113121, -0.020024895668029785, -0.0403246246278286, 0....
PMID:17417
[Problems of combined therapy with cardiac glycosides and anti-anginal drugs (author's transl)].
The rational use of cardiac glycosides and anti-anginal drugs is deduced on the basis of the pathophysiological interdependence between cardiac and coronary insufficiency. With respect to therapeutic influence on myocardial function and oxygen balance the following rules ought to be regarded: 1. The use of cardiac glyc...
[Problems of combined therapy with cardiac glycosides and anti-anginal drugs (author's transl)]. The rational use of cardiac glycosides and anti-anginal drugs is deduced on the basis of the pathophysiological interdependence between cardiac and coronary insufficiency. With respect to therapeutic influence on myocardial...
[ -0.038389336317777634, -0.012110291980206966, 0.001244179205968976, 0.04899514466524124, 0.007983529940247536, -0.06887542456388474, 0.022540150210261345, 0.01731317862868309, -0.0022640018723905087, -0.06590019911527634, -0.028553899377584457, -0.034283317625522614, -0.01469320897012949, ...
PMID:17426
Pulmonary acid aspiration syndrome: should prophylaxis be routine?
The gastric contents of three groups of patients were aspirated at the beginning and end of anaesthesia. There were 62 prepared patients, 28 emergency patients untreated and 69 prepared patients who were given 15 ml of 0.3 M sodium citrate mixture at the time of premedication. In approximately 50% of both emergency and...
Pulmonary acid aspiration syndrome: should prophylaxis be routine? The gastric contents of three groups of patients were aspirated at the beginning and end of anaesthesia. There were 62 prepared patients, 28 emergency patients untreated and 69 prepared patients who were given 15 ml of 0.3 M sodium citrate mixture at th...
[ -0.023542439565062523, -0.015733586624264717, -0.004993705078959465, 0.05137861520051956, -0.008672206662595272, 0.017158959060907364, 0.09232828766107559, 0.04450404271483421, -0.04888875409960747, -0.011689078994095325, -0.017437422648072243, -0.07267861068248749, -0.005987238138914108, ...
PMID:17427
Conditions for tracheal intubation following fazadinium and pancuronium.
Intubating conditions were studied in two groups of patients who received either fazadinium 1 mg/kg or pancuronium 0.1 mg/kg (group 1), or either fazadinium 0.5 mg/kg or pancuronium 0.08 mg/kg (group 2). In group 1 intubating conditions were studied at 30, 45, 60 and 75 s after injection of the relaxant drug, and in gr...
Conditions for tracheal intubation following fazadinium and pancuronium. Intubating conditions were studied in two groups of patients who received either fazadinium 1 mg/kg or pancuronium 0.1 mg/kg (group 1), or either fazadinium 0.5 mg/kg or pancuronium 0.08 mg/kg (group 2). In group 1 intubating conditions were studi...
[ 0.026244651526212692, -0.004455092828720808, -0.008887951262295246, 0.06731760501861572, -0.0199656430631876, 0.04953708127140999, 0.021867839619517326, 0.07729044556617737, -0.03545051068067551, -0.052007224410772324, -0.027934229001402855, -0.09475959837436676, 0.02782067470252514, 0.035...
PMID:17428
The bacteriology of skin cysts.
Thirty-nine clinically uninflamed cysts of the three most common varieties, epidermoid cysts, trichilemmal cysts and steatocystoma multiplex were removed under sterile conditions and the contents cultured under aerobic and anaerobic conditions. Seventy-three percent of epidermoid cysts grew significant numbers of organ...
The bacteriology of skin cysts. Thirty-nine clinically uninflamed cysts of the three most common varieties, epidermoid cysts, trichilemmal cysts and steatocystoma multiplex were removed under sterile conditions and the contents cultured under aerobic and anaerobic conditions. Seventy-three percent of epidermoid cysts g...
[ 0.033399175852537155, -0.0015631245914846659, -0.008401615545153618, 0.12136973440647125, 0.036568693816661835, -0.04881403222680092, 0.02815070003271103, 0.015110122039914131, -0.07639513909816742, -0.009805330075323582, 0.00994969718158245, -0.11030586808919907, 0.010059073567390442, -0....
PMID:17429
Salazopyrin in the treatment of scleroderma.
Following reports of successful treatment of various forms of scleroderma with salazopyrin, 13 selected patients were treated, 8 with acrosclerosis, one with sclerodermatomyositis and four with generalized morphoea. No effect was observed except in two cases of generalized morphoea which were in the acute stage of spre...
Salazopyrin in the treatment of scleroderma. Following reports of successful treatment of various forms of scleroderma with salazopyrin, 13 selected patients were treated, 8 with acrosclerosis, one with sclerodermatomyositis and four with generalized morphoea. No effect was observed except in two cases of generalized m...
[ 0.013712138868868351, -0.05357067659497261, 0.032659612596035004, -0.0007840811158530414, -0.028810111805796623, 0.01806464046239853, 0.029922738671302795, 0.07156407088041306, -0.06967493146657944, -0.0495099239051342, 0.013726153410971165, 0.015014850534498692, -0.023398539051413536, 0.0...
PMID:17430
The effect of zinc and pH on the behaviour of delta-aminolevulinic acid dehydratase activity in baboons exposed to lead.
Four adult male baboons (Papio ursinus) were exposed to a cloud of lead oxide dust to induce changes in the status of delta-aminolevulinic acid dehydratase (ALAD). Enzyme activity fell rapidly to a steady state as blood lead levels rose above normal. Exogenous zinc was shown to activate the enzyme, and the antagonistic...
The effect of zinc and pH on the behaviour of delta-aminolevulinic acid dehydratase activity in baboons exposed to lead. Four adult male baboons (Papio ursinus) were exposed to a cloud of lead oxide dust to induce changes in the status of delta-aminolevulinic acid dehydratase (ALAD). Enzyme activity fell rapidly to a s...
[ -0.05019793659448624, 0.05207609012722969, 0.011915413662791252, 0.039544425904750824, 0.021424418315291405, -0.05141684412956238, 0.10564985871315002, 0.039413563907146454, -0.08591171354055405, -0.01993565820157528, 0.03831329941749573, -0.08033057302236557, 0.011246555484831333, 0.03807...
PMID:17431
Beta-adrenergic receptor blocking drugs: tear lysozyme and immunological screening for adverse reaction.
Patients who had received long-term therapy with practolol and other beta-adrenergic receptor blocking drugs were examined ophthalmologically. Tear lysozyme concentration and serum autoantibodies (antinuclear antibodies, DNA-binding antibodies and intercellular cement substance antibodies) were measured. It was found t...
Beta-adrenergic receptor blocking drugs: tear lysozyme and immunological screening for adverse reaction. Patients who had received long-term therapy with practolol and other beta-adrenergic receptor blocking drugs were examined ophthalmologically. Tear lysozyme concentration and serum autoantibodies (antinuclear antibo...
[ -0.012605723924934864, 0.008663281798362732, 0.0178509671241045, 0.041485704481601715, 0.011945600621402264, -0.02863454632461071, 0.07753236591815948, 0.03140455484390259, -0.04958973079919815, -0.09567943960428238, -0.02697395719587803, 0.0058835335075855255, -0.014212079346179962, 0.079...
PMID:17432
[Study of properties of NADP malate dehydrogenase from corn leaves].
Kinetic properties of purified chloroplast isoenzyme of the "malic" enzyme from corn leaves were studied. The enzyme had optimum activity at pH 8.0 and 36 degrees C. Under standart conditions the Michaelis constants for the "malic" enzyme with Mn2+ as cofactor are 0.091 mM for malate and 0.04 mM for NADP. In case of Mg...
[Study of properties of NADP malate dehydrogenase from corn leaves]. Kinetic properties of purified chloroplast isoenzyme of the "malic" enzyme from corn leaves were studied. The enzyme had optimum activity at pH 8.0 and 36 degrees C. Under standart conditions the Michaelis constants for the "malic" enzyme with Mn2+ as...
[ -0.07534927874803543, -0.005871499888598919, 0.03629584237933159, 0.04136989265680313, 0.11767169088125229, -0.012591796927154064, 0.05786699801683426, 0.00786882545799017, -0.10265427082777023, -0.04140983521938324, 0.057190727442502975, -0.07776206731796265, 0.016542164608836174, 0.00809...
PMID:17433
[Inhibition of 4-aminobutyrate transaminase by ethanolamine-O-sulfate].
The analysis of the interaction of ethanolamine-O-sulphate with 4-aminobutyrate transaminase revealed that the inhibitory effect is exerted upon the substrate subsite of the active site of the enzyme in aldimine form. The inhibition in irreversible. The inactivation rate versus pH-curve was shown to have a sigmoid char...
[Inhibition of 4-aminobutyrate transaminase by ethanolamine-O-sulfate]. The analysis of the interaction of ethanolamine-O-sulphate with 4-aminobutyrate transaminase revealed that the inhibitory effect is exerted upon the substrate subsite of the active site of the enzyme in aldimine form. The inhibition in irreversible...
[ -0.08402401208877563, 0.021640881896018982, -0.045740481466054916, 0.06773848831653595, -0.016236452385783195, 0.021122833713889122, 0.054739102721214294, 0.04106379672884941, -0.0508158914744854, -0.028510771691799164, 0.023337440565228462, -0.01963103748857975, 0.019952578470110893, 0.09...
PMID:17434
[Possible role of acetyl-CoA-carboxylase in biosynthesis of mevalonic acid and sterols in rat liver].
Effect of citrate on acetyl-CoA incorporation into mevalonic acid, sterols and fatty acids after preliminary incubation of rat liver extracts under conditions optimal for acetyl-CoA carboxylase activation, was studied. 30 min preincubation with the citrate at 37 degrees C results in a 2--3-fold stimulation of the meval...
[Possible role of acetyl-CoA-carboxylase in biosynthesis of mevalonic acid and sterols in rat liver]. Effect of citrate on acetyl-CoA incorporation into mevalonic acid, sterols and fatty acids after preliminary incubation of rat liver extracts under conditions optimal for acetyl-CoA carboxylase activation, was studied....
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PMID:17435
[Study of betacyanin-discolorating enzyme].
An enzyme catalyzing the discoloration and breakdown of betacyanins was isolated from beet roots Beta vulgaris by centrifugation in sucrose density gradient (2.5 M, 2.0 M, 1.5 M, 1.0 M, tris-HCl buffer, 0.05 M, pH 7.2), and purified 100-fold. The enzyme activity induced the discoloration of betanin, betanidin. It was f...
[Study of betacyanin-discolorating enzyme]. An enzyme catalyzing the discoloration and breakdown of betacyanins was isolated from beet roots Beta vulgaris by centrifugation in sucrose density gradient (2.5 M, 2.0 M, 1.5 M, 1.0 M, tris-HCl buffer, 0.05 M, pH 7.2), and purified 100-fold. The enzyme activity induced the d...
[ -0.054284270852804184, 0.0031818384304642677, -0.04754944145679474, 0.025066452100872993, 0.07195456326007843, -0.006618570536375046, 0.017485331743955612, 0.05879081413149834, -0.005799600854516029, 0.0237120408564806, -0.02667427621781826, -0.07920826226472855, 0.02772223949432373, 0.049...
PMID:17436
The turnover rate of gamma-aminobutyric acid in the nuclei of telencephalon: implications in the pharmacology of antipsychotics and of a minor tranquilizer.
The turnover rate of GABA is measured in substantia nigra, globus pallidus, N. accumbens, and striatum of rats injected with muscimol, a potent GABA agonist, and diazepam. The similarity of action of the two drugs on GABA turnover further supports the theory that diazepam acts as a GABA-mimetic drug. Haloperidol and cl...
The turnover rate of gamma-aminobutyric acid in the nuclei of telencephalon: implications in the pharmacology of antipsychotics and of a minor tranquilizer. The turnover rate of GABA is measured in substantia nigra, globus pallidus, N. accumbens, and striatum of rats injected with muscimol, a potent GABA agonist, and d...
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PMID:17437
The actions of amphetamine on neurotransmitters: a brief review.
The central stimulant actions of d-amphetamine are not altered in animals in which brain stores of catecholamines have been depleted with reserpine, but they are blocked by alpha-methyltyrosine, which inhibits catecholamine synthesis. The results of a variety of experiments suggest that the central actions of amphetami...
The actions of amphetamine on neurotransmitters: a brief review. The central stimulant actions of d-amphetamine are not altered in animals in which brain stores of catecholamines have been depleted with reserpine, but they are blocked by alpha-methyltyrosine, which inhibits catecholamine synthesis. The results of a var...
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PMID:17439
Effect of heparin on complement activation and lysis of paroxysmal nocturnal hemoglobinuria (PNH) red cells.
The effect of heparin upon the binding of the third component of complement (C3) to PNH red cells in vitro and their subsequent hemolysis is described. Heparin, in increasing concentrations, progressively inhibits membrane C3 fixation and hemolysis when the classic complement pathway is activated by anti-red cell antib...
Effect of heparin on complement activation and lysis of paroxysmal nocturnal hemoglobinuria (PNH) red cells. The effect of heparin upon the binding of the third component of complement (C3) to PNH red cells in vitro and their subsequent hemolysis is described. Heparin, in increasing concentrations, progressively inhibi...
[ -0.08036026358604431, 0.016012735664844513, -0.02128509245812893, 0.026498405262827873, 0.010533658787608147, 0.006991102825850248, 0.0952242985367775, 0.019862448796629906, -0.03594309464097023, -0.022104477509856224, -0.012805628590285778, -0.04581234231591225, -0.05278512090444565, 0.00...
PMID:17440
Metabolic crowding effect in suspension of cultured lymphocytes.
We studied the respiration and glucose utilization of suspensions of cultured lymphocytes. Respiration was measured by a couloximetric method using closed systems without a gas-suspension interface. The sensitivity of the method allowed measurements during a time interval of less than 1 hr. We found that the respiratio...
Metabolic crowding effect in suspension of cultured lymphocytes. We studied the respiration and glucose utilization of suspensions of cultured lymphocytes. Respiration was measured by a couloximetric method using closed systems without a gas-suspension interface. The sensitivity of the method allowed measurements durin...
[ -0.05621820688247681, 0.027238477021455765, -0.00883565191179514, 0.013811425305902958, 0.013293803669512272, 0.020084228366613388, 0.0740402415394783, 0.0007438140455633402, -0.01847124844789505, -0.0367535836994648, 0.017511839047074318, -0.04954073578119278, 0.03861529380083084, 0.04602...
PMID:17441
Influence of H+ on K+ on adenosine-induced dilatation at pial arteries of cats.
The influence of changes in the perivascular K+ and H+ concentrations upon adenosine-induced dilatations of pial arteries was investigated by localized perivascular application using micropuncture technique. Adenosine (10(-9)-10(-3) M) was dissolved in mock spinal fluids with varying bicarbonate and potassium concentra...
Influence of H+ on K+ on adenosine-induced dilatation at pial arteries of cats. The influence of changes in the perivascular K+ and H+ concentrations upon adenosine-induced dilatations of pial arteries was investigated by localized perivascular application using micropuncture technique. Adenosine (10(-9)-10(-3) M) was ...
[ -0.0674508661031723, 0.015658661723136902, -0.015081169083714485, 0.0410187728703022, -0.019508616998791695, -0.004387222230434418, 0.03863053768873215, 0.059734851121902466, 0.0021036947146058083, -0.035282645374536514, 0.035092420876026154, -0.058934710919857025, -0.049909576773643494, -...
PMID:17444
Observations on the behaviour of barium sulphate suspensions in gastric secretion.
A number of barium sulphate suspensions showed varying patterns of stability when provoked with gastric secretion. The stability of the suspensions was affected by the pH, the mucin content and the volume of secretion used. These studies indicate that flocculation of the suspension in the presence of gastric residue de...
Observations on the behaviour of barium sulphate suspensions in gastric secretion. A number of barium sulphate suspensions showed varying patterns of stability when provoked with gastric secretion. The stability of the suspensions was affected by the pH, the mucin content and the volume of secretion used. These studies...
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PMID:17451
Cardiovascular and sympathetic response to exercise after long-term beta-adrenergic blockade.
The response to dynamic exercise was investigated in 21 patients receiving long-term treatment with beta-adrenoceptor antagonists and 22 controls. An electrocardiogram (ECG) and blood pressure were recorded before and after treadmill exercise, and plasma dopamine-beta-hydroxylase (DBH) activity was measured as an index...
Cardiovascular and sympathetic response to exercise after long-term beta-adrenergic blockade. The response to dynamic exercise was investigated in 21 patients receiving long-term treatment with beta-adrenoceptor antagonists and 22 controls. An electrocardiogram (ECG) and blood pressure were recorded before and after tr...
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PMID:17453
Release of a neurodepressing hormone from the crustacean sinus gland.
The nervous system of the crayfish contains a peptide of low molecular weight which depresses the spontaneous electrical activity of motoneurons in the abdominal ganglia. Most of this substance is contained in the sinus gland, a neurohemal organ in the eyestalk. Electrical stimulation of the isolated sinus gland, or it...
Release of a neurodepressing hormone from the crustacean sinus gland. The nervous system of the crayfish contains a peptide of low molecular weight which depresses the spontaneous electrical activity of motoneurons in the abdominal ganglia. Most of this substance is contained in the sinus gland, a neurohemal organ in t...
[ 0.02397865802049637, 0.01851869933307171, 0.03338658809661865, -0.034172747284173965, -0.023188022896647453, 0.009382901713252068, 0.054935023188591, -0.005228985566645861, -0.035591695457696915, -0.02597384713590145, 0.004355847369879484, -0.042081620544195175, 0.06185753270983696, 0.0558...
PMID:17455
The blockade of serotonin uptake into synaptosomes:relationship to an interaction with monoamine oxidase inhibitors.
To test the hypothesis that the hyperpyrexia produced by meperidine and detromethorphan in rabbits pretreated with a monoamine oxidase inhibitor is related to inhibition of neuronal uptake of serotonin (5-hydroxytryptamine (5-HT)), fluoxetine (Lilly 110140) was studied. This potent and specific 5-HT neuronal uptake blo...
The blockade of serotonin uptake into synaptosomes:relationship to an interaction with monoamine oxidase inhibitors. To test the hypothesis that the hyperpyrexia produced by meperidine and detromethorphan in rabbits pretreated with a monoamine oxidase inhibitor is related to inhibition of neuronal uptake of serotonin (...
[ -0.039233267307281494, -0.018352603539824486, -0.009919765405356884, 0.0437711700797081, -0.04034432768821716, 0.026275010779500008, 0.07150869816541672, 0.023517396301031113, -0.00008007090946193784, -0.0831722840666771, -0.022797759622335434, -0.0005433163023553789, 0.013410544022917747, ...
PMID:17456
Multiple forms of DNA-dependent RNA and polyadenylic acid polymerases from heterotrophically grown Rhodospirillum rubrum.
Three, two major and one minor, distinct RNA polymerases have been isolated and partially purified from heterotrophically grown Rhodospirillum rubrum, a facultative photosynethetic bacterium. Associated with each of these three enzymes is a distinct polyadenylic acid polyemrase. All of these enzyme activities are depen...
Multiple forms of DNA-dependent RNA and polyadenylic acid polymerases from heterotrophically grown Rhodospirillum rubrum. Three, two major and one minor, distinct RNA polymerases have been isolated and partially purified from heterotrophically grown Rhodospirillum rubrum, a facultative photosynethetic bacterium. Associ...
[ -0.05542520806193352, -0.03434302285313606, -0.06833283603191376, 0.017170758917927742, 0.0821269303560257, 0.007756746374070644, -0.010764265432953835, 0.03809484839439392, -0.04883788153529167, -0.01976136676967144, 0.04000433161854744, -0.03210199251770973, 0.0016249737236648798, -0.039...
PMID:17457
Preenrichment procedure for detection of Salmonella in gelatin.
Trypticase soy broth was superior to nutrient and lactose broths as a preenrichment medium for the detection of Salmonella in artificially and naturally contaiminated gelatin. The detection rate for Salmonella were further enhanced when homogenization of the gelatin-broth mixture was accomplished by the use of gelatina...
Preenrichment procedure for detection of Salmonella in gelatin. Trypticase soy broth was superior to nutrient and lactose broths as a preenrichment medium for the detection of Salmonella in artificially and naturally contaiminated gelatin. The detection rate for Salmonella were further enhanced when homogenization of t...
[ -0.030677566304802895, -0.027791960164904594, -0.009960331954061985, -0.05035809427499771, 0.0691656842827797, -0.00862368755042553, 0.054498739540576935, 0.049548547714948654, -0.007482609245926142, -0.02414577268064022, -0.026040729135274887, -0.05772916227579117, -0.005498114041984081, ...
PMID:17458
The cellular role of nitrogen in the biosynthesis of alkaloids by submerged culture of Claviceps purpurea (Fr.) Tul.
Asparagine was a superior nitrogen source for clavine-alkaloid production in Claviceps purpurea. Its transport into the cell excedded the cell's biosynthetic need for this amino acid. Asparagine entered the cell without degradation. This disturbed the relative pool sizes of various amino acids resulting in a change in ...
The cellular role of nitrogen in the biosynthesis of alkaloids by submerged culture of Claviceps purpurea (Fr.) Tul. Asparagine was a superior nitrogen source for clavine-alkaloid production in Claviceps purpurea. Its transport into the cell excedded the cell's biosynthetic need for this amino acid. Asparagine entered ...
[ -0.022635072469711304, -0.016525276005268097, -0.034400325268507004, -0.027262801304459572, -0.043261945247650146, 0.008579226210713387, 0.02844354324042797, 0.030129723250865936, -0.05986097827553749, 0.025127217173576355, 0.035887107253074646, -0.09243646264076233, 0.04551570117473602, 0...
PMID:17459
Clostridium perfringens--specific lysin.
A mitomycin C induced lysate of Clostridium perfringens strain KZ219 was lytic to 50 strains of C. perfringens of types A-E, and three strains of C. plagarum. The lysin was active against only 2 out of 87 strains of 51 other clostridial species. The optimum pH of the lytic agent was 5.5. The activity was largely inacti...
Clostridium perfringens--specific lysin. A mitomycin C induced lysate of Clostridium perfringens strain KZ219 was lytic to 50 strains of C. perfringens of types A-E, and three strains of C. plagarum. The lysin was active against only 2 out of 87 strains of 51 other clostridial species. The optimum pH of the lytic agent...
[ -0.04736028611660004, -0.00857304036617279, -0.04195866361260414, 0.05389997363090515, 0.022053467109799385, -0.12300878763198853, 0.00675782049074769, 0.019770286977291107, -0.02708107978105545, -0.03467831760644913, 0.00409695552662015, -0.03732673078775406, -0.0034716217778623104, 0.016...
PMID:17460
Effects of the herbicides fluometuron and prometryn of Rhizoctonia solani in soil cultures.
Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activities were highly correlated with amounts of mycelium in soil. Both enzyme activities were redu...
Effects of the herbicides fluometuron and prometryn of Rhizoctonia solani in soil cultures. Responses of Rhizoctonia solani to herbicides in soil cultures were assessed by measuring soil enzyme activity and other growth-related factors. Both beta-galactosidase (EC 3.2.1.23) and phosphatase (EC 3.1.3.1.3.1.3.2) activiti...
[ 0.018826819956302643, -0.018516045063734055, 0.01880972646176815, 0.06781808286905289, 0.0741540864109993, -0.03296475112438202, 0.022054679691791534, 0.06398044526576996, -0.04377222806215286, -0.03229033201932907, -0.008196056820452213, 0.0030740329530090094, 0.03791790083050728, 0.03817...
PMID:17461
Suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed Vibrio parahaemolyticus.
Vibrio parahaemolyticus cells were injured by chilling and heating, and their recovery was tested in glucose-salt-Teepol broth (GSTB), tryptic soy broth containing 7% NaCl (TSBS), Horie - arabinose - ethyl violet broth (HAEB), and water blue - alizarin yellow broth (WBAY). Exponential phase cells were more sensitive t...
Suitability of some enrichment broths and diluents for enumerating cold- and heat-stressed Vibrio parahaemolyticus. Vibrio parahaemolyticus cells were injured by chilling and heating, and their recovery was tested in glucose-salt-Teepol broth (GSTB), tryptic soy broth containing 7% NaCl (TSBS), Horie - arabinose - eth...
[ -0.04584208503365517, -0.003446121932938695, -0.028136326000094414, 0.00919546652585268, 0.07532919943332672, 0.005355316214263439, 0.044093839824199677, 0.05526416748762131, -0.04477059841156006, -0.037846874445676804, -0.024719061329960823, -0.06345213949680328, 0.06651358306407928, 0.00...
PMID:17462
Inhibiton by sulfanilamide of sporulation in Saccharomyces cerevisiae.
The antimetabolite sulfanilamide inhibits sporulation in Saccharomyces cerevisiae strain AP1. Cells exposed to sulfanilamide at various times during the sporulation process become progressively insensitive to the drug, although accumulation of sulfanilamide by the cells increases with time. Vegetative growth of AP1 is ...
Inhibiton by sulfanilamide of sporulation in Saccharomyces cerevisiae. The antimetabolite sulfanilamide inhibits sporulation in Saccharomyces cerevisiae strain AP1. Cells exposed to sulfanilamide at various times during the sporulation process become progressively insensitive to the drug, although accumulation of sulfa...
[ 0.001633519190363586, 0.016204919666051865, -0.02064734324812889, -0.011005698703229427, -0.03839084506034851, 0.020381327718496323, 0.014731194823980331, -0.01843315362930298, -0.022848088294267654, -0.006593450903892517, 0.03771577030420303, -0.04787340760231018, 0.0838066041469574, 0.01...
PMID:17463
Enzymatic hydrolysis of agar: purification and characterization of beta-neoagarotetraose hydrolase from Pseudomonas atlantica.
Agarose is degraded by a beta-agarase from Pseudomonas atlantica to neoagarooligosaccharides of degree of polymerization (DP), 4, 6, 8, and 10. A beta-neoagarotetraose hydrolase cleaves the central beta-linkage in neoagarotetraose and the beta-linkage near the nonreducing end in neoagarohexaose and -octaose to yield ne...
Enzymatic hydrolysis of agar: purification and characterization of beta-neoagarotetraose hydrolase from Pseudomonas atlantica. Agarose is degraded by a beta-agarase from Pseudomonas atlantica to neoagarooligosaccharides of degree of polymerization (DP), 4, 6, 8, and 10. A beta-neoagarotetraose hydrolase cleaves the cen...
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PMID:17464
Properties of 1-phosphofructokinase from Pseudomonas putida.
The 1-phosphofructokinase (1-PFK, EC 2.7.1.56) from Pseudomonas putida was partially purified by a combination of (NH4)2SO4 fractionation and DEAE-Sephadex column chromatography. In its kinetic properties, this enzyme resembled the 1-PFK's from other bacteria. With the substrates fructose-1-phosphate (F-1-P) and adenos...
Properties of 1-phosphofructokinase from Pseudomonas putida. The 1-phosphofructokinase (1-PFK, EC 2.7.1.56) from Pseudomonas putida was partially purified by a combination of (NH4)2SO4 fractionation and DEAE-Sephadex column chromatography. In its kinetic properties, this enzyme resembled the 1-PFK's from other bacteria...
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PMID:17465
Concentration of enteroviruses from large volumes of turbid estuary water.
A method is described for the efficient concentration of viruses from large volumes of highly turbid estuary water. Virus in acidified seawater in the presence of aluminum chloride is adsorbed to a 10-in. (about 25.4 cm) fibreglass depth cartridge and 2- and 0.65-micron epoxy-fibreglass filters in series. This filter s...
Concentration of enteroviruses from large volumes of turbid estuary water. A method is described for the efficient concentration of viruses from large volumes of highly turbid estuary water. Virus in acidified seawater in the presence of aluminum chloride is adsorbed to a 10-in. (about 25.4 cm) fibreglass depth cartrid...
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PMID:17466
Isolation and characterization of a neuroblastoma cell line from peripheral blood in a patient with disseminated disease.
Circulation in the blood stream of neuroblastoma cells was confirmed by establishment of a cell line from the peripheral blood of a child with disseminated disease. The morphologic, enzymatic, and chromosomal pattern of this cell line was similar to a cell line established from the primary tumor on a previous occasion....
Isolation and characterization of a neuroblastoma cell line from peripheral blood in a patient with disseminated disease. Circulation in the blood stream of neuroblastoma cells was confirmed by establishment of a cell line from the peripheral blood of a child with disseminated disease. The morphologic, enzymatic, and c...
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PMID:17468
Subcellular compartmentation of creatine kinase isoenzymes in guinea pig heart.
In an attempt to determine whether a subcellular compartmentation of creatine kinase exists and if so, whether there is a different distribution of the 3 isoenzymes of CK in the cell, studies were carried out with the guinea pig heart which had been subfractionated by either isopycnic density gradient centrifugation or...
Subcellular compartmentation of creatine kinase isoenzymes in guinea pig heart. In an attempt to determine whether a subcellular compartmentation of creatine kinase exists and if so, whether there is a different distribution of the 3 isoenzymes of CK in the cell, studies were carried out with the guinea pig heart which...
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PMID:17467
Some new approaches to potential test systems for drugs against prostatic cancer.
We have previously reported on test systems, based on 5alpha-reductase (5alpha-RA) and arginase activities and steroid deposition in animal prostates, potentially useful in screening drugs possibly effective in cancer of the prostate. Recently, we have concentrated on the development of other in vivo and in vitro syste...
Some new approaches to potential test systems for drugs against prostatic cancer. We have previously reported on test systems, based on 5alpha-reductase (5alpha-RA) and arginase activities and steroid deposition in animal prostates, potentially useful in screening drugs possibly effective in cancer of the prostate. Rec...
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PMID:17474
Aurinetricarboxylic acid: a potent inhibitor of glucose-6-phosphate dehydrogenase.
Inhibition by aurinetricarboxylic acid (ATA) of glucose-6-phosphate (G6P) dehydrogenase was "competitive" with respect to G6P and "mixed type" with respect to NADP+. Inhibited enzyme bound two molecules of ATA. Kinetic constants, Km, Ki at varying pH suggested possible binding of the inhibitor by the sulfhydryl of the ...
Aurinetricarboxylic acid: a potent inhibitor of glucose-6-phosphate dehydrogenase. Inhibition by aurinetricarboxylic acid (ATA) of glucose-6-phosphate (G6P) dehydrogenase was "competitive" with respect to G6P and "mixed type" with respect to NADP+. Inhibited enzyme bound two molecules of ATA. Kinetic constants, Km, Ki ...
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PMID:17477
[The effect of toxin II isolated from sea anemones (Anemonia sulcata) on the isolated guinea pig ileum].
When the guinea-pig isolated ileum is treated with the Toxin II from Anemonia sulcata, it releases not only acetylcholine but also serotonin. These transmitters are both involved in the spasmogenic action of the toxin.
[The effect of toxin II isolated from sea anemones (Anemonia sulcata) on the isolated guinea pig ileum]. When the guinea-pig isolated ileum is treated with the Toxin II from Anemonia sulcata, it releases not only acetylcholine but also serotonin. These transmitters are both involved in the spasmogenic action of the tox...
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PMID:17478
[Demonstration of N-acetylgalactosaminyltransferase activity in the murine lymphoma YC8].
The N-acetyl-galactosaminyltransferase activity has been studied in the biological fluids of YC8-lymphoma bearing Balb/c mice. It is enhanced during tumor development from 1 to 30 times in peritoneal fluids and from 1 to 10 times in sera. This activity is nil in urines. Optimal requirements for activity have been deter...
[Demonstration of N-acetylgalactosaminyltransferase activity in the murine lymphoma YC8]. The N-acetyl-galactosaminyltransferase activity has been studied in the biological fluids of YC8-lymphoma bearing Balb/c mice. It is enhanced during tumor development from 1 to 30 times in peritoneal fluids and from 1 to 10 times ...
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PMID:17480
Continuous-flow determination of dialyzable calcium in serum.
We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is fluorometrically measured. Analysis of samples containing protein...
Continuous-flow determination of dialyzable calcium in serum. We describe a procedure for measuring dialyzable calcium in 60 sera per hour with use of AutoAnalyzer (Technicon) modules. The serum is buffered, then dialyzed in a regular 6-inch (15-cm) dialysis block. A constant proportion of the dialyzable calcium is flu...
[ -0.04184938222169876, 0.03174185752868652, 0.039570778608322144, 0.002963680075481534, -0.05365964397788048, -0.042236655950546265, 0.07918091118335724, 0.06857887655496597, -0.04766037315130234, -0.009105111472308636, 0.0016117481281980872, -0.10003630816936493, 0.0038070324808359146, 0.0...
PMID:17481
Continuous-flow determination of serum inorganic phosphate with a single reagent--the vanadomolybdate method re-evaluated.
The merits of the vanadomolybdate method in the determination of inorganic phosphate are highly underestimated with regard to the simplicity of the method and the stability of both the reagents and the color that is formed. The absorption curve of the phosphovanadomolybdate complex, with its maximum at 335 nm, extends ...
Continuous-flow determination of serum inorganic phosphate with a single reagent--the vanadomolybdate method re-evaluated. The merits of the vanadomolybdate method in the determination of inorganic phosphate are highly underestimated with regard to the simplicity of the method and the stability of both the reagents and...
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PMID:17482
Simultaneous measurement of phenobarbital, phenytoin, primidone, ethosuximide, and carbamazepine in serum by high-pressure liquid chromatography.
We present a method for simultaneously determining five anticonvulsants [phenobarbital, phenytoin (diphenylhydantoin), primidone, ethosuximide, and carbamazepine] in as little as 25 microliters of serum. The proteins are precipitated with an acetonitrile solution containing hexobarbital as an internal standard. The ant...
Simultaneous measurement of phenobarbital, phenytoin, primidone, ethosuximide, and carbamazepine in serum by high-pressure liquid chromatography. We present a method for simultaneously determining five anticonvulsants [phenobarbital, phenytoin (diphenylhydantoin), primidone, ethosuximide, and carbamazepine] in as littl...
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PMID:17479
Children who do not talk at school.
This paper contains two case reports illustrating the use of behaviour modification in the treatment of elective mutism. The programme was carried out by two social workers in the natural environment of the home and school.
Children who do not talk at school. This paper contains two case reports illustrating the use of behaviour modification in the treatment of elective mutism. The programme was carried out by two social workers in the natural environment of the home and school.
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PMID:17485
Recent developments in the clinical assessment of the metabolism of aromatics by high-performance, reversed-phase chromatography with amperometric detection.
High-performance liquid chromatography with electrochemical detection has developed into a tool with excellent capability to monitor picomole amounts of individual metabolites in both tissue and body-fluid specimens. Significant technical advances in the area of microparticle chemically-bonded stationary phases have le...
Recent developments in the clinical assessment of the metabolism of aromatics by high-performance, reversed-phase chromatography with amperometric detection. High-performance liquid chromatography with electrochemical detection has developed into a tool with excellent capability to monitor picomole amounts of individua...
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PMID:17486
Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-beta-naphthylamine derivatives.
Enzyme activity can be measured in single cells or in cell suspensions by either static or flow microfluorometry when a fluorogenic substrate is used. We have used amino acid derivatives of arylamines as fluorogenic substrates for tagging cellular proteinases. The liberated aromatic amine, which can diffuse from the ce...
Flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-beta-naphthylamine derivatives. Enzyme activity can be measured in single cells or in cell suspensions by either static or flow microfluorometry when a fluorogenic substrate is used. We have used amino acid derivatives of arylami...
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PMID:17488
Inorganic phosphorus in human bile.
The inorganic phosphorus concentration has been measured in fresh bile from patients undergoing cholecystectomy, usually because of cholelithiasis. The amount in common duct bile for patients with cholesterol on the stone surface was significantly higher than that for patients with some calcium carbonate in this area. ...
Inorganic phosphorus in human bile. The inorganic phosphorus concentration has been measured in fresh bile from patients undergoing cholecystectomy, usually because of cholelithiasis. The amount in common duct bile for patients with cholesterol on the stone surface was significantly higher than that for patients with s...
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PMID:17489
A practical chromogenic procedure for the diagnosis of Krabbe's disease.
Krabbe's disease is caused by a deficiency of galactocerebrosidase in organs and tissues. Determinations of galactocerebrosidase activity had required the use of galactocerebroside labeled with radiocarbon or radiohydrogen. These materials are expensive and their use is restricted to laboratories with radioactive count...
A practical chromogenic procedure for the diagnosis of Krabbe's disease. Krabbe's disease is caused by a deficiency of galactocerebrosidase in organs and tissues. Determinations of galactocerebrosidase activity had required the use of galactocerebroside labeled with radiocarbon or radiohydrogen. These materials are exp...
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PMID:17490
Urinary alpha-glucosidase analysis for the detection of the adult form of Pompe's disease.
Two alpha-glucosidases from human heart, liver, muscle, kidney and urine have been separated by means of Sephadex G-100 gel filtration. The first peak (Peak I) was neutral alpha-glucosidase and the second peak (Peak II) was lysosomal acid alpha-glucosidase. Peak II was absent in a patient with the adult form of Pompe's...
Urinary alpha-glucosidase analysis for the detection of the adult form of Pompe's disease. Two alpha-glucosidases from human heart, liver, muscle, kidney and urine have been separated by means of Sephadex G-100 gel filtration. The first peak (Peak I) was neutral alpha-glucosidase and the second peak (Peak II) was lysos...
[ -0.021473674103617668, -0.033473797142505646, 0.050005823373794556, -0.01594497635960579, 0.03415613994002342, -0.05235995352268219, 0.05818049609661102, 0.05976179242134094, 0.020645668730139732, -0.03506038337945938, 0.03080461546778679, -0.0942821353673935, -0.020775964483618736, 0.0340...
PMID:17491
Serum gamma-glutamyl transferase activity in chronic renal failure during regular hemodialysis and after successful renal transplantation.
Serum gamma-glutamyl transferase activity (gamma-GT) was measured in 108 uraemic patients, 110 patients on regular haemodialysis and 71 sucessfully transplanted patients. The incidence of abnormal results was 11, 14 and 28%, respectively. The results in the non-dialyzed and dialyzed patients indicate that in uraemia se...
Serum gamma-glutamyl transferase activity in chronic renal failure during regular hemodialysis and after successful renal transplantation. Serum gamma-glutamyl transferase activity (gamma-GT) was measured in 108 uraemic patients, 110 patients on regular haemodialysis and 71 sucessfully transplanted patients. The incide...
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PMID:17493
Interaction of calcium ions with serum albumin in chronic renal failure.
In order to study the effect of the uremic environment on serum calcium-albumin interaction, ten adult subjects with chronic renal failure (creatinine clearances below 20 ml/min) and five adult normal subjects, had their serum ionic calcium (Orion SS-20) determined at three different serum pH values. At similar serum p...
Interaction of calcium ions with serum albumin in chronic renal failure. In order to study the effect of the uremic environment on serum calcium-albumin interaction, ten adult subjects with chronic renal failure (creatinine clearances below 20 ml/min) and five adult normal subjects, had their serum ionic calcium (Orion...
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PMID:17494
A micro-radiochemical assay for alpha-1,4-glucosidase and its use in the assessment of type II glycogenosis (Pompe's disease).
An assay for alpha-1,4-glucosidase (acid maltase) activity which is deficient in Pompe's disease is described. The assay can be used to measure the enzyme in cultured skin fibroblasts, cultured amniotic cells and peripheral blood leucocytes. [U-14 C]Maltose is used as the substrate in a total assay volume of 8 microlit...
A micro-radiochemical assay for alpha-1,4-glucosidase and its use in the assessment of type II glycogenosis (Pompe's disease). An assay for alpha-1,4-glucosidase (acid maltase) activity which is deficient in Pompe's disease is described. The assay can be used to measure the enzyme in cultured skin fibroblasts, cultured...
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