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PMID:17947 | A physician extender training program based on clinical algorithms. | The ability of physician extenders to provide patient care in a variety of settings has been reported widely. Less attention has been paid to training programs, especially those of short duration, for physician extenders. A three-month training program for physician extenders at a large teaching hospital was based on t... | A physician extender training program based on clinical algorithms. The ability of physician extenders to provide patient care in a variety of settings has been reported widely. Less attention has been paid to training programs, especially those of short duration, for physician extenders. A three-month training program... | [
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PMID:17948 | [Aspartate transfer across the blood-retina barrier (author's transl)]. | Permeability of the blood-brain barrier is restricted with respect to amino acids involved in neurotransmission. This finding is well-documented in the case of gamma-amino-butyric acid (GABA) and glycine. Aspartic acid, which is also considered to be a transmitter, equally does not cross the blood-brain barrier in the ... | [Aspartate transfer across the blood-retina barrier (author's transl)]. Permeability of the blood-brain barrier is restricted with respect to amino acids involved in neurotransmission. This finding is well-documented in the case of gamma-amino-butyric acid (GABA) and glycine. Aspartic acid, which is also considered to ... | [
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PMID:17949 | [Recent concepts of respiratory regulation (author's transl)]. | There are two functional aspects of the respiratory control system, the one being adaptation of ventilation to metabolic needs and the other acid-base homeostasis of the extracellular fluid of the brain. These two functions are perfectly compatible with one another under normal conditions. During hypoxia or hyperthermi... | [Recent concepts of respiratory regulation (author's transl)]. There are two functional aspects of the respiratory control system, the one being adaptation of ventilation to metabolic needs and the other acid-base homeostasis of the extracellular fluid of the brain. These two functions are perfectly compatible with one... | [
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PMID:17951 | [Bistability in the activity of glutamine synthetase in ammonium-limiting chemostat cultures of Escherichia coli ML 30]. | The approximative estimation of the function micron([NH+4]) in cultures of E. coli ML 30 had shown that bistability of the ammonium concentration in ammonium limited continuous cultures could be possible (BERGTER et al. 1977). This phenomenon suggested a bistability in the regulation of ammonia assimilation. Therefore,... | [Bistability in the activity of glutamine synthetase in ammonium-limiting chemostat cultures of Escherichia coli ML 30]. The approximative estimation of the function micron([NH+4]) in cultures of E. coli ML 30 had shown that bistability of the ammonium concentration in ammonium limited continuous cultures could be poss... | [
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PMID:17957 | [The problems of adverse effects to the liver of alpha-methyldopa]. | In 20 patients who were treated with alpha-methyldopa since at least one year the bilirubin content, the activities of aspartate and alanine-aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase as well as the retention of bromsulphalein were determined. In altogether 10 patients pathological finding... | [The problems of adverse effects to the liver of alpha-methyldopa]. In 20 patients who were treated with alpha-methyldopa since at least one year the bilirubin content, the activities of aspartate and alanine-aminotransferase, alkaline phosphatase and gamma-glutamyl transpeptidase as well as the retention of bromsulpha... | [
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PMID:17963 | [Relationship between thermal treatment of milk and hysteresis of the milk protein system. II. Studies on casein micelles (author's transl)]. | The association of colloidal calcium phosphate with the casein micelles is the principal reason for hysteresis of the milk protein system. Because of this association the secondary calcium phosphate cannot be titrated at its pK = 7.2, but only at lower pH values depending on the previous thermal treatment of the milk. ... | [Relationship between thermal treatment of milk and hysteresis of the milk protein system. II. Studies on casein micelles (author's transl)]. The association of colloidal calcium phosphate with the casein micelles is the principal reason for hysteresis of the milk protein system. Because of this association the seconda... | [
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PMID:17958 | Regulation and characterization of L-serine: pyruvate aminotransferase in rat liver cytosol and mitochondria. | Distribution of rat liver serine: pyruvate aminotransferase between cytosol and mitochondria varies considerably with the dietary and hormonal state of animals. Feeding a high-protein diet or fasting the animals results in an increase in the enzyme activity of both fractions but more marked in the mitochondrial fractio... | Regulation and characterization of L-serine: pyruvate aminotransferase in rat liver cytosol and mitochondria. Distribution of rat liver serine: pyruvate aminotransferase between cytosol and mitochondria varies considerably with the dietary and hormonal state of animals. Feeding a high-protein diet or fasting the animal... | [
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PMID:17959 | Identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate. | State and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. Complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. From the pH dependence of the photooxidation... | Identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate. State and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. Complete i... | [
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PMID:17964 | [On the enzymatic breakdown of tripolyphosphate and diphosphate in comminuted meat. VII. Influence of sodium chloride on the tripolyphosphatase and diphosphatase activity in bovine muscle (author's transl)]. | Sodium chloride activates the tripolyphosphatase of the muscle tissue. Optimum activity occurs at 4--5 percent NaC1. The increase in activity seems to be due to the effect of C1-ions. NaC1 inhibits the tripolyphosphatase activity of muscle press juice. NaC1 decreases the total diphosphatase activity of muscle tissue as... | [On the enzymatic breakdown of tripolyphosphate and diphosphate in comminuted meat. VII. Influence of sodium chloride on the tripolyphosphatase and diphosphatase activity in bovine muscle (author's transl)]. Sodium chloride activates the tripolyphosphatase of the muscle tissue. Optimum activity occurs at 4--5 percent N... | [
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PMID:17965 | [On the enzymatic breakdown of tripolyphosphate and diphosphate in comminuted meat. VIII. Influence of divalent cations on the tripolyphosphatase activity of muscle tissue (author's transl)]. | The tripolyphosphatase of comminuted muscle tissue is activated by MgC1(2) and ethylenediamine tetraacetate and inhibited by CaC1(2) and diphosphate. These results are discussed with reference to the formation of an inactive enzyme-substrate complex. | [On the enzymatic breakdown of tripolyphosphate and diphosphate in comminuted meat. VIII. Influence of divalent cations on the tripolyphosphatase activity of muscle tissue (author's transl)]. The tripolyphosphatase of comminuted muscle tissue is activated by MgC1(2) and ethylenediamine tetraacetate and inhibited by CaC... | [
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PMID:17983 | Factors influencing L-asparaginase production by staphylococci. | Cultural and nutritional requirements for a maximum synthesis of 1-asparaginase by staphylococci were determined. The best production of the enzyme was found in the stationary phase of growth of a batch culture. The highest 1-asparaginase yield was obtained when the culture were aerated during an exponential phase of g... | Factors influencing L-asparaginase production by staphylococci. Cultural and nutritional requirements for a maximum synthesis of 1-asparaginase by staphylococci were determined. The best production of the enzyme was found in the stationary phase of growth of a batch culture. The highest 1-asparaginase yield was obtaine... | [
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PMID:17984 | Subcellular distribution and properties of alkaline inorganic pyrophosphatase of maize leaves. | 1. Studies on the distribution of alkaline inorganic pyrophosphatase (pyrophosphate phosphohydrolase, EC 3.6.1.1) in the subcellular fractions of maize leaves showed that the enzyme is present in cytoplasm, chloroplasts and mitochondria. The activity observed in nuclei and microsomes may result from contamination with ... | Subcellular distribution and properties of alkaline inorganic pyrophosphatase of maize leaves. 1. Studies on the distribution of alkaline inorganic pyrophosphatase (pyrophosphate phosphohydrolase, EC 3.6.1.1) in the subcellular fractions of maize leaves showed that the enzyme is present in cytoplasm, chloroplasts and m... | [
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PMID:17985 | Acid phosphatase of the yeast Rhodotorula rubra. Purification and properties of the enzyme. | 1. Acid phosphatase from the yeast Rhodotorula rubra was purified 44-fold. The purification procedure involved mechanical disruption of cells, precipitation with ethanol, chromatography on DEAE- and CM-cellulose. 2. The purified enzyme is homogeneous in polyacrylamide gels at pH 4.5, 9.5 and 8.4. Carbohydrate content a... | Acid phosphatase of the yeast Rhodotorula rubra. Purification and properties of the enzyme. 1. Acid phosphatase from the yeast Rhodotorula rubra was purified 44-fold. The purification procedure involved mechanical disruption of cells, precipitation with ethanol, chromatography on DEAE- and CM-cellulose. 2. The purified... | [
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PMID:17986 | [Proof of guanylate cyclase activity in the coronary artery of cattle]. | Guanylate cyclase activities were identified in a soluble fraction and a particular fraction obtained from the Arteria coronaria of cattle. The Km-value was 1.0 +/- 0.7 - 10(-4) M for the enzyme substrate complex of the guanylate cyclase of the soluble fraction and 9.2 +/- 1.5 - 10(-4) M for the particular fraction. Fo... | [Proof of guanylate cyclase activity in the coronary artery of cattle]. Guanylate cyclase activities were identified in a soluble fraction and a particular fraction obtained from the Arteria coronaria of cattle. The Km-value was 1.0 +/- 0.7 - 10(-4) M for the enzyme substrate complex of the guanylate cyclase of the sol... | [
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PMID:17987 | [Demonstration of protein kinase activities in the coronary artery of cattle]. | Protein kinase activities were identified in a soluble and a particulate fraction from the A. coronaria of cattle. For both protein kinase activities Mg++ is essential. Protamine was used as a substrate of the protein kinase activity of the soluble fraction. The pH optimum of the protein kinase activity of the soluble ... | [Demonstration of protein kinase activities in the coronary artery of cattle]. Protein kinase activities were identified in a soluble and a particulate fraction from the A. coronaria of cattle. For both protein kinase activities Mg++ is essential. Protamine was used as a substrate of the protein kinase activity of the ... | [
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PMID:17988 | Effect of tilorone hydrochloride on graft-versus-host (GvH) reaction in mice. | The effects of tilorone hydrochloride (TH), a powerful interferonogenic agent exhibiting also immune modulatory properties, on GvH reaction was studied, using the popliteal lymph node assay in mice. Administration of TH at ten days 0 or +2 relative to cell transfer to recipient mice led to a significant dose-dependent ... | Effect of tilorone hydrochloride on graft-versus-host (GvH) reaction in mice. The effects of tilorone hydrochloride (TH), a powerful interferonogenic agent exhibiting also immune modulatory properties, on GvH reaction was studied, using the popliteal lymph node assay in mice. Administration of TH at ten days 0 or +2 re... | [
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PMID:17989 | [An ion exchange method to determine free metal concentrations, adapted for use in biological fluids: methods and determination of (Mg2+)]. | An ion exchange method for measuring concentrations of free (ionized) metal ions and its application to the determination of [Mg2+] is described. A surface sulfonated polystyrene material is used as the "twodimensional" cation exchanger. The sample-volume is 1 ml. About 50 determinations can be performed within 1 hr ha... | [An ion exchange method to determine free metal concentrations, adapted for use in biological fluids: methods and determination of (Mg2+)]. An ion exchange method for measuring concentrations of free (ionized) metal ions and its application to the determination of [Mg2+] is described. A surface sulfonated polystyrene m... | [
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PMID:17990 | Pulmonary complications, ventilation and blood gases after upper abdominal surgery. | Forty patients who underwent elective cholecystectomy were examined preoperatively and during the first postoperative week by physical examination, measurement of FVC and FEV1, arterial pH and blood gas analyses, and chest x-ray. Postoperative pulmonary complications (p.p.c.) were detected in 30 (75%) of the patients. ... | Pulmonary complications, ventilation and blood gases after upper abdominal surgery. Forty patients who underwent elective cholecystectomy were examined preoperatively and during the first postoperative week by physical examination, measurement of FVC and FEV1, arterial pH and blood gas analyses, and chest x-ray. Postop... | [
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PMID:17991 | The cerebrovascular CO2 reactivity during the acute phase of brain injury. | Using the intra-arterial 133xenon (133Xe) method, the cerebrovascular response to acute Paco2 reduction was studied in 26 unconscious, brain-injured patients subjected to controlled ventilation. The CO2 reactivity was calculated as delta in CBF/delta Paco2. The perfusion pressure was defined as the difference between m... | The cerebrovascular CO2 reactivity during the acute phase of brain injury. Using the intra-arterial 133xenon (133Xe) method, the cerebrovascular response to acute Paco2 reduction was studied in 26 unconscious, brain-injured patients subjected to controlled ventilation. The CO2 reactivity was calculated as delta in CBF/... | [
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PMID:17992 | The pressure-flow relations of the canine brain in acute mechanically induced arterial hypertension at different levels of cerebral blood flow. | Cerebral pressure-flow relations were studied in anaesthetized dogs during acute arterial hypertension, induced by compression of the thoracic aorta. In one group of animals steady state measurements were made with the radioactive gas elimination method. In another group the immediate changes of blood flow at a blood p... | The pressure-flow relations of the canine brain in acute mechanically induced arterial hypertension at different levels of cerebral blood flow. Cerebral pressure-flow relations were studied in anaesthetized dogs during acute arterial hypertension, induced by compression of the thoracic aorta. In one group of animals st... | [
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PMID:17993 | Peroperative arterial blood pressure control in ophthalmological anaesthesia. | The effects were studied of a standardised general anaesthesia on mean arterial blood pressure, central venous pressure, blood gas values and the course of cataract operations. The material consisted of 20 unselected senile cataract operations, the mean age of the patients being 71.3 years. The arterial blood pressure ... | Peroperative arterial blood pressure control in ophthalmological anaesthesia. The effects were studied of a standardised general anaesthesia on mean arterial blood pressure, central venous pressure, blood gas values and the course of cataract operations. The material consisted of 20 unselected senile cataract operation... | [
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PMID:17994 | Effects upon the fetus of oxygen administration to the mother. A study in monkey. | Catheters were placed into assorted arteries and veins of 8 anaesthetized pregnant monkeys and their fetuses. Oxygen-sensitive electrodes were also inserted subcutaneously into 3 of the 8 fetuses. Periodic samples of maternal and fetal blood were analyzed for PO2, PCO2 and pH. Oxygen administration to the mothers relia... | Effects upon the fetus of oxygen administration to the mother. A study in monkey. Catheters were placed into assorted arteries and veins of 8 anaesthetized pregnant monkeys and their fetuses. Oxygen-sensitive electrodes were also inserted subcutaneously into 3 of the 8 fetuses. Periodic samples of maternal and fetal bl... | [
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PMID:17995 | The finding that secretory component is not associated with gamma-glutamyltranspeptidase activity. | The gamma-glutamyltranspeptidase activity of human milk was concentrated by ammonium sulphate precipitation. On gel chromatography of the dissolved precipitate, the activity was eluted in the high-molecular-weight fraction containing secretory IgA, while no activity appeared in the eluate at the position of free secret... | The finding that secretory component is not associated with gamma-glutamyltranspeptidase activity. The gamma-glutamyltranspeptidase activity of human milk was concentrated by ammonium sulphate precipitation. On gel chromatography of the dissolved precipitate, the activity was eluted in the high-molecular-weight fractio... | [
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PMID:17996 | Atenolol in the treatment of angina pectoris. | Nineteen men, aged 41-64 years, with stable angina pectoris have completed a random double-blind study of atenolol, 50 mg b.i.d., atenolol, 100 mg b.i.d., and placebo. Fifteen patients had subjective improvement on atenolol, two were unchanged and two felt worse (because of asthenia/leg fatigue). No significant placebo... | Atenolol in the treatment of angina pectoris. Nineteen men, aged 41-64 years, with stable angina pectoris have completed a random double-blind study of atenolol, 50 mg b.i.d., atenolol, 100 mg b.i.d., and placebo. Fifteen patients had subjective improvement on atenolol, two were unchanged and two felt worse (because of... | [
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PMID:18002 | Acute myocardial infarction and ischemic injury during surgery for coronary artery disease. | The incidence of myocardial infarction, acute ischemic injury, and associated serum enzyme abnormalities has been evaluated in four operations involving the coronary circulation. The highest incidence of infarction was associated with internal mammary implantation (Vineberg procedure). There was no significant differen... | Acute myocardial infarction and ischemic injury during surgery for coronary artery disease. The incidence of myocardial infarction, acute ischemic injury, and associated serum enzyme abnormalities has been evaluated in four operations involving the coronary circulation. The highest incidence of infarction was associate... | [
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PMID:18003 | Isolated coronary periarteritis: report of a case of unexpected death in a young pregnant woman. | A case of sudden unexpected death in a 23 year old woman in her 8th month of pregnancy is reported. Death occurred from isolated coronary periarteritis with thrombosis. It is believed that the thrombosis was secondary to the adventitial infiltrate, which was of a type suggesting a hypersensitivity factor. It is propose... | Isolated coronary periarteritis: report of a case of unexpected death in a young pregnant woman. A case of sudden unexpected death in a 23 year old woman in her 8th month of pregnancy is reported. Death occurred from isolated coronary periarteritis with thrombosis. It is believed that the thrombosis was secondary to th... | [
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PMID:18004 | An isozyme of erythrocyte pyruvate kinase (PK-Los Angeles) with impaired kinetics corrected by fructose-1, 6-diphosphate. | A mutant isozyme of erythrocyte pyruvate kinase was found in a family of French-Canadian ancestry in association with hemolytic anemia. The isozyme was characterized by normal maximal activity, pH optimum, heat stability, and fructosediphosphate activation constants, but had markedly reduced affinity for the substrate,... | An isozyme of erythrocyte pyruvate kinase (PK-Los Angeles) with impaired kinetics corrected by fructose-1, 6-diphosphate. A mutant isozyme of erythrocyte pyruvate kinase was found in a family of French-Canadian ancestry in association with hemolytic anemia. The isozyme was characterized by normal maximal activity, pH o... | [
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PMID:18005 | Bacteremia in hospitalized children. | The results of 6,951 blood cultures taken from hospitalized children are reviewed. One or more organisms grew from 6% (399) of the cultures, of which 189 (two thirds) were considered to represent confirmed bacteremia. The most common organisms associated with bacteremia were Haemophilus influenzae, Streptococcus pneumo... | Bacteremia in hospitalized children. The results of 6,951 blood cultures taken from hospitalized children are reviewed. One or more organisms grew from 6% (399) of the cultures, of which 189 (two thirds) were considered to represent confirmed bacteremia. The most common organisms associated with bacteremia were Haemoph... | [
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PMID:18007 | Effects of cimetidine, a histamine H2-receptor antagonist, on various experimental gastric and duodenal ulcers. | The effects of cimetidine, a new histamine H2-receptor antagonist, on the development of experimental gastric and duodenal ulcers were studied. It was found that either by the oral, intraduodenal, or intraperitoneal route this agent had a marked inhibitory activity on stress-, aspirin-, indomethacin-, or histamine-indu... | Effects of cimetidine, a histamine H2-receptor antagonist, on various experimental gastric and duodenal ulcers. The effects of cimetidine, a new histamine H2-receptor antagonist, on the development of experimental gastric and duodenal ulcers were studied. It was found that either by the oral, intraduodenal, or intraper... | [
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PMID:18008 | Quantitative determination of four antihistamines in combination by high-pressure liquid chromatography. | A procedure used to assay four antihistamines--brompheniramine maleate, chlorpheniramine maleate, pheniramine maleate and pyrilamine maleate in combination--is discussed. The method requires a high-pressure liquid chromatograph and an intermediate polarity column. The method is accurate and appears to be stability-indi... | Quantitative determination of four antihistamines in combination by high-pressure liquid chromatography. A procedure used to assay four antihistamines--brompheniramine maleate, chlorpheniramine maleate, pheniramine maleate and pyrilamine maleate in combination--is discussed. The method requires a high-pressure liquid c... | [
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PMID:18009 | Bladder dysfunction after radical abdominal hysterectomy. | Bladder dysfunction is a common occurrence following radical hysterectomy. We studied bladder function prospectively in 10 patients before and after radical hysterectomy. Results suggest that the hypertonic phase observed immediately postoperatively is the result of an increase in myogenic tonicity of the detrusor musc... | Bladder dysfunction after radical abdominal hysterectomy. Bladder dysfunction is a common occurrence following radical hysterectomy. We studied bladder function prospectively in 10 patients before and after radical hysterectomy. Results suggest that the hypertonic phase observed immediately postoperatively is the resul... | [
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PMID:18010 | Effects of prolonged infusion of beta-adrenergic agonists on uterine and umbilical blood flow in pregnant sheep. | We have extended our evaluation of the effects of three beta-adrenergic agents in near-term pregnant sheep to include a period of infusion three times longer than previously studied. This extension has provided the following information: (1) Initial depression of uterine blood flow and mean arterial pressure associated... | Effects of prolonged infusion of beta-adrenergic agonists on uterine and umbilical blood flow in pregnant sheep. We have extended our evaluation of the effects of three beta-adrenergic agents in near-term pregnant sheep to include a period of infusion three times longer than previously studied. This extension has provi... | [
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PMID:18011 | Thymic involution in murine graft-versus-host reaction. Epithelial injury mimicking human thymic dysplasia. | Mild, moderate, and severe graft-versus-host (GVH) reactions were induced in four series of experiments in 71 CBA X A and C57BL/6 X A F1 hybrid mice. At regular intervals post-GVH reaction induction (Days 4-42), the animals were sacrificed, autopsied, and histologically studied. Visceral alterations of GVH reaction wer... | Thymic involution in murine graft-versus-host reaction. Epithelial injury mimicking human thymic dysplasia. Mild, moderate, and severe graft-versus-host (GVH) reactions were induced in four series of experiments in 71 CBA X A and C57BL/6 X A F1 hybrid mice. At regular intervals post-GVH reaction induction (Days 4-42), ... | [
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PMID:18013 | Thyroid cold acclimation influences on norepinephrine metabolism in brown fat. | Norepinephrine turnover rates and tyrosine hydroxylase activities were determined in the interscapular brown fat pad of the rat during cold acclimation, hyperthyroxinism, and after thyroidectomy. Rats were cold acclimated by placement in a cold room, one rat to a cage, for a period of 6 wk. Hyperthyroxinism was induced... | Thyroid cold acclimation influences on norepinephrine metabolism in brown fat. Norepinephrine turnover rates and tyrosine hydroxylase activities were determined in the interscapular brown fat pad of the rat during cold acclimation, hyperthyroxinism, and after thyroidectomy. Rats were cold acclimated by placement in a c... | [
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PMID:18014 | Hexose monophosphate shunt in isolated cardiac myocytes from normal rats. | The activity of the hexose monophosphate shunt was studied in myocytes obtained from the ventricles of normal, adult, male rats. When myocytes were incubated in buffer containing either 1-14C- or 6-14C-labeled glucose the ratio of C-1/C-6 14CO2 evolved was essentially unity. The addition of plasma levels of amino acids... | Hexose monophosphate shunt in isolated cardiac myocytes from normal rats. The activity of the hexose monophosphate shunt was studied in myocytes obtained from the ventricles of normal, adult, male rats. When myocytes were incubated in buffer containing either 1-14C- or 6-14C-labeled glucose the ratio of C-1/C-6 14CO2 e... | [
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PMID:18015 | HCO3- and H+ secretion in rat ileum in vivo. | Replacing Cl- in the perfusion fluids decreased the luminal pH of rat ileum perfused in vivo. Varying the initial pH in Cl- depletion studies indicated an equilibrium pH near 6.6. Na+ depletion in Cl- deleted fluids partially inhibited luminal acidification. Addition of HCO3- accelerated Na+ absorption and decreased Cl... | HCO3- and H+ secretion in rat ileum in vivo. Replacing Cl- in the perfusion fluids decreased the luminal pH of rat ileum perfused in vivo. Varying the initial pH in Cl- depletion studies indicated an equilibrium pH near 6.6. Na+ depletion in Cl- deleted fluids partially inhibited luminal acidification. Addition of HCO3... | [
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PMID:18016 | Neural control of glycogen content and its diurnal rhythm in mouse pineal cell. | In adult male dd mice, possible mechanisms regulating the glycogen content in the pineal cell were investigated by a semiquantitative histochemical method, with particular reference to the role of the sympathetic innervation. Reserpine, superior cervical ganglionectomy (SCGX), or decentralization of the ganglia (DC), a... | Neural control of glycogen content and its diurnal rhythm in mouse pineal cell. In adult male dd mice, possible mechanisms regulating the glycogen content in the pineal cell were investigated by a semiquantitative histochemical method, with particular reference to the role of the sympathetic innervation. Reserpine, sup... | [
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PMID:18017 | Function of human platelets during extracorporeal circulation. | The interaction between human platelets and nonbiologic surfaces was studied during in vitro recirculation of 500 ml of fresh, heparinized human blood in four different perfusion circuits. Circuits differed in surface area (0.1 m2 or 0.9 m2) and in surface composition. No important differences were observed between sta... | Function of human platelets during extracorporeal circulation. The interaction between human platelets and nonbiologic surfaces was studied during in vitro recirculation of 500 ml of fresh, heparinized human blood in four different perfusion circuits. Circuits differed in surface area (0.1 m2 or 0.9 m2) and in surface ... | [
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PMID:18018 | Development of SHR hypertension and cardiac hypertrophy during prolonged beta blockade. | Spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats were treated with beta-adrenergic receptor inhibiting drugs (either propranolol or timolol) from conception until 12 weeks of age to determine if this therapy would alter the development of systemic hypertension or left ventricular hypertrophy. Therapy (propr... | Development of SHR hypertension and cardiac hypertrophy during prolonged beta blockade. Spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats were treated with beta-adrenergic receptor inhibiting drugs (either propranolol or timolol) from conception until 12 weeks of age to determine if this therapy would alter ... | [
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PMID:18019 | Effects of increased intracranial pressure on pulmonary vascular resistance of fetal and neonatal goats. | The effects of increased intracranial pressure on the pulmonary circulation were investigated in fetal and neonatal goats. Pulmonary vascular resistance and systemic arterial pressure increased with elevation of intracranial pressure in neonatal animals. Alpha-adrenergic blockade completely eliminated both of these res... | Effects of increased intracranial pressure on pulmonary vascular resistance of fetal and neonatal goats. The effects of increased intracranial pressure on the pulmonary circulation were investigated in fetal and neonatal goats. Pulmonary vascular resistance and systemic arterial pressure increased with elevation of int... | [
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PMID:18020 | Active alkalinization by amphibian gastric fundic mucosa in vitro. | Gastric fundic mucosae in vitro from four species of frog and Necturus secrete HCO-3 at a steady-state rate of 0.25-0.55 microneq-cm-2-h-1 which corresponds to 5-10% of maximal H+ secretion. Net alkalinization was quantitated in mucosae with spontaneously resting H+ secretion or in mucosae inhibited by histamine H2-re... | Active alkalinization by amphibian gastric fundic mucosa in vitro. Gastric fundic mucosae in vitro from four species of frog and Necturus secrete HCO-3 at a steady-state rate of 0.25-0.55 microneq-cm-2-h-1 which corresponds to 5-10% of maximal H+ secretion. Net alkalinization was quantitated in mucosae with spontaneou... | [
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PMID:18021 | Pancreatic bicarbonate, serum gastrin, and secretin responses to meals varying in pH. | The role of secretin in the postprandial bicarbonate response by the pancreas is not clear. This study reports secretin and bicarbonate secretion after exogenous and endogenous acidification of meal. In dogs with gastric and pancreatic fistulas, a liver extract meal at various pH levels was introduced into the stomach ... | Pancreatic bicarbonate, serum gastrin, and secretin responses to meals varying in pH. The role of secretin in the postprandial bicarbonate response by the pancreas is not clear. This study reports secretin and bicarbonate secretion after exogenous and endogenous acidification of meal. In dogs with gastric and pancreati... | [
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PMID:18022 | Influence of aldosterone on renal ammonia production. | Administering D-aldosterone, 7 microgram 100 g-1, to rats results in a marked rise in ammonium excretion and metabolic alkalosis. Increased ammonium excretion is not related to either a significant elevation in potassium excretion nor to hypokalemia. Consequently, potassium depletion does not appear to be the causative... | Influence of aldosterone on renal ammonia production. Administering D-aldosterone, 7 microgram 100 g-1, to rats results in a marked rise in ammonium excretion and metabolic alkalosis. Increased ammonium excretion is not related to either a significant elevation in potassium excretion nor to hypokalemia. Consequently, p... | [
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PMID:18023 | Solute permeability of the alveolar epithelium in acute hemodynamic pulmonary edema in dogs. | Ten anesthetized dogs, 48 h postintravenous 131I-albumin injection, had a segment of lung airspace isolated by a balloon-tipped catheter lodged in a bronchus. An isotonic saline solution containing trace amounts of Blue Dextran, 125I-albumin, and 57Co-cyanocobalamin was instilled into the lung segment. During control p... | Solute permeability of the alveolar epithelium in acute hemodynamic pulmonary edema in dogs. Ten anesthetized dogs, 48 h postintravenous 131I-albumin injection, had a segment of lung airspace isolated by a balloon-tipped catheter lodged in a bronchus. An isotonic saline solution containing trace amounts of Blue Dextran... | [
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PMID:18024 | Prostaglandin metabolism in the kidneys of spontaneously hypertensive rats. | Renal prostaglandins have been implicated in the regulation of blood pressure. We have therefore compared prostaglandin metabolism in the kidneys of spontaneously hypertensive rats (SHR's) of the Aoki-Okamoto strain and normotensive Wistar-Kyoto (WKY) controls. The microsomal fraction of the renal medulla contained mos... | Prostaglandin metabolism in the kidneys of spontaneously hypertensive rats. Renal prostaglandins have been implicated in the regulation of blood pressure. We have therefore compared prostaglandin metabolism in the kidneys of spontaneously hypertensive rats (SHR's) of the Aoki-Okamoto strain and normotensive Wistar-Kyot... | [
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PMID:18025 | Properties of the laryngeal chemoreflex in neonatal piglets. | Cardiorespiratory reflex responses to laryngeal chemoreceptor stimulation were studied in 62 piglets of both sexes varying in age from 1 to 79 days. The distal trachea was cannulated to provide a free airway and the proximal end used to introduce fluids into the laryngeal area. Introduction of either water or milk prod... | Properties of the laryngeal chemoreflex in neonatal piglets. Cardiorespiratory reflex responses to laryngeal chemoreceptor stimulation were studied in 62 piglets of both sexes varying in age from 1 to 79 days. The distal trachea was cannulated to provide a free airway and the proximal end used to introduce fluids into ... | [
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PMID:18027 | [The aging alimentary tract (author's transl)]. | The physiological process of aging causes changes in volumen and composition of saliva, gastric and pancreatic juice and bile flow, a diminished absorption and an altered bacterial flora. However, these changes are compensated and do not cause symptoms. Besides of an increased disposition to develop carcinoma of the gu... | [The aging alimentary tract (author's transl)]. The physiological process of aging causes changes in volumen and composition of saliva, gastric and pancreatic juice and bile flow, a diminished absorption and an altered bacterial flora. However, these changes are compensated and do not cause symptoms. Besides of an incr... | [
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PMID:18028 | [Differentiation of the aging intestinal epithelium by morphology, histochemistry and discelectrophoresis (author's transl)]. | The intestinal epithelium of the small and large intestine of fetal rats (19th, 20th and 21st day of pregnancy) newborn and juvenile rats (1st to 50th day of life) adult rats (3rd to 4th month) and senile rats (18th to 30th month) was investigated by means of morphology, enzyme histochemistry and discelectrophoresis. T... | [Differentiation of the aging intestinal epithelium by morphology, histochemistry and discelectrophoresis (author's transl)]. The intestinal epithelium of the small and large intestine of fetal rats (19th, 20th and 21st day of pregnancy) newborn and juvenile rats (1st to 50th day of life) adult rats (3rd to 4th month) ... | [
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PMID:18029 | [Gastroscopic findings in aged patients (author's transl)]. | Endoscopic findings in a group of 169 aged patients are shown to be concentrated in the stomach, whereas pathologic findings in the duodenal bulb are less frequent. In comparison to a group of young patients gastric mucosal atrophy, gastric ulcer, carcinoma of the operated and unoperated stomach, polyps are predominant... | [Gastroscopic findings in aged patients (author's transl)]. Endoscopic findings in a group of 169 aged patients are shown to be concentrated in the stomach, whereas pathologic findings in the duodenal bulb are less frequent. In comparison to a group of young patients gastric mucosal atrophy, gastric ulcer, carcinoma of... | [
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PMID:18030 | [Gastroduodenal ulcers in patients of a geriatric hospital (author's transl)]. | Among 3646 patients of a geriatric hospital we we found 7,82% = 285 patients who suffered once in their life from a gastroduodenal ulcer. We noted: 1. gastroduodenal scars in 3,3% = 121 cases, 2. anamnestic proved gastroduodenal ulcer disease in 1,5% = 57 cases, 3. clinical and/or pathological proved gastroduodenal ulc... | [Gastroduodenal ulcers in patients of a geriatric hospital (author's transl)]. Among 3646 patients of a geriatric hospital we we found 7,82% = 285 patients who suffered once in their life from a gastroduodenal ulcer. We noted: 1. gastroduodenal scars in 3,3% = 121 cases, 2. anamnestic proved gastroduodenal ulcer diseas... | [
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PMID:18031 | [Ischemic bowel disease (author's transl)]. | Geriatric patients are preferentially involved in ischemic bowel disease. The sudden occlusion of the large mesenteric arteries (a. mesenterica superior (more frequently) and inferior) is followed by intestinal gangrene and peritonitis with a poor prognosis and a high letality (greater than 90%). In chronic intestinal ... | [Ischemic bowel disease (author's transl)]. Geriatric patients are preferentially involved in ischemic bowel disease. The sudden occlusion of the large mesenteric arteries (a. mesenterica superior (more frequently) and inferior) is followed by intestinal gangrene and peritonitis with a poor prognosis and a high letalit... | [
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PMID:18032 | [Dependence of diverticulosis on age (author's transl)]. | The diverticulosis of the gastrointestinal tract is a widespread senile disease. The dependence of the diverticulosis on age is investigated on the basis of 1 000 proven cases of this disease. The importance of the anatomicomorphological lesions of the organs at old age as its cause is pointed out. The males seem to be... | [Dependence of diverticulosis on age (author's transl)]. The diverticulosis of the gastrointestinal tract is a widespread senile disease. The dependence of the diverticulosis on age is investigated on the basis of 1 000 proven cases of this disease. The importance of the anatomicomorphological lesions of the organs at ... | [
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PMID:18033 | [Functional digestive troubles in old age (author's transl)]. | Functional disorders are frequent in elderly patients. However the high incidence of organic lesions in advanced age demands a most careful examination. Each diagnosis of functional disorders made by exclusion of organic lesions has to be revised, if symptoms persist or symptomatic treatment remains inefficient. Gastro... | [Functional digestive troubles in old age (author's transl)]. Functional disorders are frequent in elderly patients. However the high incidence of organic lesions in advanced age demands a most careful examination. Each diagnosis of functional disorders made by exclusion of organic lesions has to be revised, if symptom... | [
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PMID:18035 | Age-dependent alterations of the rate of RNA synthesis in rat brain cell nuclei. | Endogenous Mn2+ and Mg2+ activated RNA polymerase activity was measured in isolated cell nuclei of brain from rats of different age-groups. It was established that the activity of the nucleoplasmic RNA polymerase is maintained at the level of young animals up to an age of 16 months but is decreased after 24 months. The... | Age-dependent alterations of the rate of RNA synthesis in rat brain cell nuclei. Endogenous Mn2+ and Mg2+ activated RNA polymerase activity was measured in isolated cell nuclei of brain from rats of different age-groups. It was established that the activity of the nucleoplasmic RNA polymerase is maintained at the level... | [
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PMID:18034 | [Nutritional problems in old age (author's transl)]. | While age is increasing involutiones specific to age can be proofed at the gastrointestinal tract, which lead to a deceleration but quantitative unchanged adsorption. Wrong nutrition concerning to the take up of fat, carbohydrates, proteins and trace elements can be found in greater age very often. By watching a colle... | [Nutritional problems in old age (author's transl)]. While age is increasing involutiones specific to age can be proofed at the gastrointestinal tract, which lead to a deceleration but quantitative unchanged adsorption. Wrong nutrition concerning to the take up of fat, carbohydrates, proteins and trace elements can be ... | [
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PMID:18036 | [In vitro-phosphorylation of histones in chromatin of various tissues in relation to age (author's transl)]. | The influence of age on the structure of chromatin of various mammalian cells with modest or terminated mitotic activity has been examined. For this purpose chromatin from dog skeletal muscle, and human neuronal and glial cells, has been incubated together with an exogenous histone phosphokinase and ATP-gamma32P, and t... | [In vitro-phosphorylation of histones in chromatin of various tissues in relation to age (author's transl)]. The influence of age on the structure of chromatin of various mammalian cells with modest or terminated mitotic activity has been examined. For this purpose chromatin from dog skeletal muscle, and human neuronal... | [
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PMID:18037 | [RNA metabolism and ageing (author's transl)]. | The labelling behaviour of RNA fractions in rat liver after application of 32P-orthophosphate and 14C-orotate in vivo in young (3-5 months) and old animals (20 months) was presented. Whereas the tracer incorporation in old animals 60 minutes after 32P-application seems to be decreased we observed increased specific act... | [RNA metabolism and ageing (author's transl)]. The labelling behaviour of RNA fractions in rat liver after application of 32P-orthophosphate and 14C-orotate in vivo in young (3-5 months) and old animals (20 months) was presented. Whereas the tracer incorporation in old animals 60 minutes after 32P-application seems to ... | [
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PMID:18038 | [The vascularisation of the granulation tissue in different ages. - Its importance for wound healing (author's transl)]. | The part of capillaries of a plastic sponge granuloma was determined in rats of different ages. It was found that granulation tissue of older rats contains significantly fewer capillaries than that of young animals. The diminished vascularisation of granulation tissue may be responsible for the deterioration of wound h... | [The vascularisation of the granulation tissue in different ages. - Its importance for wound healing (author's transl)]. The part of capillaries of a plastic sponge granuloma was determined in rats of different ages. It was found that granulation tissue of older rats contains significantly fewer capillaries than that o... | [
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PMID:18039 | [Histone phosphorylation of human lymphocytes after PHA stimulation in relation to age (author's transl)]. | Lymphocytes of young and old donors are stimulated by phytohemagglutinin (PHA). The 32P-incorporation into the histones which occurs in the early phase after PHA-stimulation is determined. In lymphocytes of old persons the 32P-incorporation in the histones is diminished by about 50% with respect to the cells of young d... | [Histone phosphorylation of human lymphocytes after PHA stimulation in relation to age (author's transl)]. Lymphocytes of young and old donors are stimulated by phytohemagglutinin (PHA). The 32P-incorporation into the histones which occurs in the early phase after PHA-stimulation is determined. In lymphocytes of old pe... | [
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PMID:18040 | [Hydroxylation of skin collagen in relation to age: analyses of the human skin]. | The results of human skin analysis are demonstrated. It has been pointed out, that the content of hydroxyproline and hydroxylysine, in addition to the usual variation as the other aminoacids varies extensively. The extent of hydroxylation was estimated from the relation of OH-aminoacids to (not hydroxylated) each other... | [Hydroxylation of skin collagen in relation to age: analyses of the human skin]. The results of human skin analysis are demonstrated. It has been pointed out, that the content of hydroxyproline and hydroxylysine, in addition to the usual variation as the other aminoacids varies extensively. The extent of hydroxylation ... | [
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PMID:18041 | [Investigations about the effect of run-training and run-stress on glucose tolerance and insulin secretion with ageing streptozotocin-diabetic rats (author's transl)]. | Untrained grown-up and old rats with a mild Streptozotocin-diabetes show in i.v. glucose tolerance test a pathological glucose assimilation and diminished insulin secretion in comparison to control rats of the same age after a maximal run-stress. Trained rats show a different behaviour in glucose tolerance test dependi... | [Investigations about the effect of run-training and run-stress on glucose tolerance and insulin secretion with ageing streptozotocin-diabetic rats (author's transl)]. Untrained grown-up and old rats with a mild Streptozotocin-diabetes show in i.v. glucose tolerance test a pathological glucose assimilation and diminish... | [
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PMID:18042 | [Determination of neurophysiological parameters of the aging CNS. I. Evoked potentials (author's transl)]. | Somatosensory (SEP) and visual (VEP) evoked potentials were studied in 65 healthy subjects who ranged in age from 62-91 years and in 48 normal persons aged 18-38 years. The amplitudes and latencies of the different components of the evoked potentials were measured. The transmission of evoked potentials from the specifi... | [Determination of neurophysiological parameters of the aging CNS. I. Evoked potentials (author's transl)]. Somatosensory (SEP) and visual (VEP) evoked potentials were studied in 65 healthy subjects who ranged in age from 62-91 years and in 48 normal persons aged 18-38 years. The amplitudes and latencies of the differen... | [
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PMID:18065 | [Lens changes occuring as a result of lowered pH (acidosis) (author's transl)]. | In the lens, disorders of the metabolism occur, and, with them, active permeability (the cation pump with uptake of K and release of Na) changes to passive permeability and consequently Na ions enter with water. As a result, the lens increases in weight and a subcapsular (permeability) cataract develops. It is shown th... | [Lens changes occuring as a result of lowered pH (acidosis) (author's transl)]. In the lens, disorders of the metabolism occur, and, with them, active permeability (the cation pump with uptake of K and release of Na) changes to passive permeability and consequently Na ions enter with water. As a result, the lens increa... | [
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PMID:18069 | [The present state of hypotension (author's transl)]. | The study was made of the recent literature on controlled hypotension and its place in modern anaesthetic practice. The effects of the different techniques and drugs are outlined in this paper. The results of investigation revealed that the circulation of heart, brain, liver, and kidneys is able to tolerate a wide rang... | [The present state of hypotension (author's transl)]. The study was made of the recent literature on controlled hypotension and its place in modern anaesthetic practice. The effects of the different techniques and drugs are outlined in this paper. The results of investigation revealed that the circulation of heart, bra... | [
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PMID:18070 | [Cardiovascular effects of flunitrazepam (rohypnol, RO-5-4200) (author's transl)]. | We reported on the haemodynamic effects of 0.03 mg/kg flunitrazepam during surgical procedures in neuroleptanalgesia in 39 patients with congenital or acquired heart diseases, functional class II-IV. The benzodiazepine derivative did not cause any relevant effect on the inotropic state of the myocardium. There were onl... | [Cardiovascular effects of flunitrazepam (rohypnol, RO-5-4200) (author's transl)]. We reported on the haemodynamic effects of 0.03 mg/kg flunitrazepam during surgical procedures in neuroleptanalgesia in 39 patients with congenital or acquired heart diseases, functional class II-IV. The benzodiazepine derivative did not... | [
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PMID:18071 | Intramuscular lorazepam versus pentobarbital premedication: a comparison of patient sedation, anxiolysis, and recall. | In a double-blind, controlled study in 40 veterans, IM lorazepam was significantly superior to IM pentobarbital as a preoperative sedative and in ability to suppress the memory of events of the operative day. A trend was noted (not statistically in this small series) toward greater patient acceptance of lorazepam. No i... | Intramuscular lorazepam versus pentobarbital premedication: a comparison of patient sedation, anxiolysis, and recall. In a double-blind, controlled study in 40 veterans, IM lorazepam was significantly superior to IM pentobarbital as a preoperative sedative and in ability to suppress the memory of events of the operativ... | [
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PMID:18075 | New techniques to measure blood cholinesterase activity in domesticated animals. | A macromethod and a semimicromethod were developed to measure erythrocyte acetylcholinesterase activity in cattle, sheep, goats, horses, dogs, and swine, and to measure plasma cholinesterase activity in horses, dogs, and swine. Comparison of the 2 methods with erythrocytes of sheep, cattle, goats, and horses indicated ... | New techniques to measure blood cholinesterase activity in domesticated animals. A macromethod and a semimicromethod were developed to measure erythrocyte acetylcholinesterase activity in cattle, sheep, goats, horses, dogs, and swine, and to measure plasma cholinesterase activity in horses, dogs, and swine. Comparison ... | [
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PMID:18072 | Selection of the source of mixed venous blood samples in severely traumatized patients. | The technic of balloon flotation catheterization represents a significant advance in providing an additional aid to diagnosis, clinical assessment, and management of the critically ill. The fact that such patients are admitted to an intensive care unit (ICU) invariably presumes that their management will include close ... | Selection of the source of mixed venous blood samples in severely traumatized patients. The technic of balloon flotation catheterization represents a significant advance in providing an additional aid to diagnosis, clinical assessment, and management of the critically ill. The fact that such patients are admitted to an... | [
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PMID:18078 | Drug therapy in the management of asthma. | In the past decade there has been substantial improvement in the quality of drug therapy for asthma. In part, this reflects the greater sophistication and confidence in the use of bronchodilator drugs, after their role in modifying the intracellular concentration of cyclic nucleotides was discovered. Another advance ha... | Drug therapy in the management of asthma. In the past decade there has been substantial improvement in the quality of drug therapy for asthma. In part, this reflects the greater sophistication and confidence in the use of bronchodilator drugs, after their role in modifying the intracellular concentration of cyclic nucl... | [
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PMID:18079 | [Behavior therapy in disorders of dietary behavior]. | Behaviour therapies using conditioning principles have been successful in the treatment of some psychopatological eating behaviours. Such have been the cases for anorexia nervosae in adolescents and adults, refusal to eat in the young child and difficulties of swallowing. Some of these cases are described. Research has... | [Behavior therapy in disorders of dietary behavior]. Behaviour therapies using conditioning principles have been successful in the treatment of some psychopatological eating behaviours. Such have been the cases for anorexia nervosae in adolescents and adults, refusal to eat in the young child and difficulties of swallo... | [
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PMID:18081 | Isoelectric focusing of hog cholera virus. | The isoelectric point of Hog Cholera virus (Alfort strain) has been determined (pHi = 4.8). A clear stimulation of its infectivity can be recorded at this pH. The isoelectrophoretic profiles of strains isolated in the field show no significant differences. | Isoelectric focusing of hog cholera virus. The isoelectric point of Hog Cholera virus (Alfort strain) has been determined (pHi = 4.8). A clear stimulation of its infectivity can be recorded at this pH. The isoelectrophoretic profiles of strains isolated in the field show no significant differences. | [
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PMID:18082 | [Spectrophotometric determination of rifampicin S in the presence of rifampicin O]. | The limits of the use of the methods of direct photometric and differential spectrophotometric determination of rifamycin S containing rifamycin O as an admixture were discussed. The method of direct photometry did not provide determination of rifamycin S in the presence of rifamycin O since instability of the latter u... | [Spectrophotometric determination of rifampicin S in the presence of rifampicin O]. The limits of the use of the methods of direct photometric and differential spectrophotometric determination of rifamycin S containing rifamycin O as an admixture were discussed. The method of direct photometry did not provide determina... | [
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PMID:18083 | Azlocillin: in vitro studies of a new semisynthetic penicillin. | The activity of azlocillin, a new semisynthetic penicillin, was determined against 582 clinical isolates of gram-negative bacilli and gram-positive cocci. Over 75% of the isolates of Pseudomonas aeruginosa were inhibited at a concentration of 12.5 mug or less per ml. Azlocillin is also active against indole-negative an... | Azlocillin: in vitro studies of a new semisynthetic penicillin. The activity of azlocillin, a new semisynthetic penicillin, was determined against 582 clinical isolates of gram-negative bacilli and gram-positive cocci. Over 75% of the isolates of Pseudomonas aeruginosa were inhibited at a concentration of 12.5 mug or l... | [
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PMID:18084 | Phosphate inhibition of secondary metabolism in Serratia marcescens. | The synthesis of prodigiosin by non-proliferating cells of Serratia marcescens was examined in the presence of a wide range of concentrations of inorganic phosphate (Pi). A high elevation of pigment formation was obtained at less than or equal to 0.3 mM, and a broader but much lower elevation was obtained at 10 to 250 ... | Phosphate inhibition of secondary metabolism in Serratia marcescens. The synthesis of prodigiosin by non-proliferating cells of Serratia marcescens was examined in the presence of a wide range of concentrations of inorganic phosphate (Pi). A high elevation of pigment formation was obtained at less than or equal to 0.3 ... | [
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PMID:18085 | Effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and pH. | Fungi (Rhizopus stolonifer, Trichoderma viride, Fusarium oxysporum f. sp. conglutinans, Cunninghamella echinulata, and several species of Aspergillus and Penicillium) tolerated higher concentrations of cadmium (Cd) when grown in soil than when grown on laboratory media, indicating that soil mitigated the toxic effects ... | Effect of cadmium on fungi and on interactions between fungi and bacteria in soil: influence of clay minerals and pH. Fungi (Rhizopus stolonifer, Trichoderma viride, Fusarium oxysporum f. sp. conglutinans, Cunninghamella echinulata, and several species of Aspergillus and Penicillium) tolerated higher concentrations of ... | [
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PMID:18086 | Significance of fecal coliform-positive Klebsiella. | A total of 191 Klebsiella pneumoniae isolates of human clinical, bovine mastitis, and a wide variety of environmental sources were tested for fecal coliform (FC) response with the membrane filtration and most probable number techniques. Twenty-seven Escherichia coli cultures of human clinical and environmental origins ... | Significance of fecal coliform-positive Klebsiella. A total of 191 Klebsiella pneumoniae isolates of human clinical, bovine mastitis, and a wide variety of environmental sources were tested for fecal coliform (FC) response with the membrane filtration and most probable number techniques. Twenty-seven Escherichia coli c... | [
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PMID:18087 | Purification and characteristics of an alginase from Alginovibrio aquatilis. | An exocellular inducible alginase from a strain of Alginovibrio aquatilis was purified 61-fold by ammonium sulfate precipitation and column chromatography on Sephadex G-150 and diethylaminoethyl-cellulose. The purified enzyme was more resistant than the crude enzyme to elevated temperatures. The monovalent cations Cs+,... | Purification and characteristics of an alginase from Alginovibrio aquatilis. An exocellular inducible alginase from a strain of Alginovibrio aquatilis was purified 61-fold by ammonium sulfate precipitation and column chromatography on Sephadex G-150 and diethylaminoethyl-cellulose. The purified enzyme was more resistan... | [
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PMID:18088 | Concentration of enteroviruses from estuarine water. | Pleated cartridge filters readily adsorb viruses in estuarine water at low pH containing aluminum chloride. Adsorbed viruses are efficiently recovered by treating filters with glycine buffer at high pH. By using these procedures, it was possible to recover approximately 70% of the poliovirus added to 400 liters of estu... | Concentration of enteroviruses from estuarine water. Pleated cartridge filters readily adsorb viruses in estuarine water at low pH containing aluminum chloride. Adsorbed viruses are efficiently recovered by treating filters with glycine buffer at high pH. By using these procedures, it was possible to recover approximat... | [
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PMID:18116 | Bucolome in prevention of hyperbilirubinaemia in preterm infants. | Fifty preterm babies were randomly assigned to a group given oral bucolome (30 mg/kg per day for 5 days) and a control group. Serum bilirubin levels of the treated infants from day 4 onwards were consistently lower than those of the control infants. 3 of the 25 control infants (but none of the 25 treated infants) had b... | Bucolome in prevention of hyperbilirubinaemia in preterm infants. Fifty preterm babies were randomly assigned to a group given oral bucolome (30 mg/kg per day for 5 days) and a control group. Serum bilirubin levels of the treated infants from day 4 onwards were consistently lower than those of the control infants. 3 of... | [
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PMID:18119 | Prospective study of the Robertshaw endobronchial catheter in thoracic surgery. | One hundred three men undergoing thoracotomy on a general thoracic surgery service received endobronchial anesthesia with 100% oxygen using the Robertshaw tube. Bronchial intubation was accomplished in all. However, cross-leak or difficulty with deflation necessitated discontinuance in 8, while Pao2 values of 41 and 45... | Prospective study of the Robertshaw endobronchial catheter in thoracic surgery. One hundred three men undergoing thoracotomy on a general thoracic surgery service received endobronchial anesthesia with 100% oxygen using the Robertshaw tube. Bronchial intubation was accomplished in all. However, cross-leak or difficulty... | [
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PMID:18125 | [The cellulase enzyme system during growth and development of Acanthamoeba castellanii (author's transl)]. | It could be shown that extracts of growing cultures of Acanthamoeba castellanii contained a cellulose degrading system. Reducing sugars are split off by one component of this system at an optimum of pH 4, another enzyme changes the viscosity at an optimum of pH 6, and a third component is a beta-glucosidase with an opt... | [The cellulase enzyme system during growth and development of Acanthamoeba castellanii (author's transl)]. It could be shown that extracts of growing cultures of Acanthamoeba castellanii contained a cellulose degrading system. Reducing sugars are split off by one component of this system at an optimum of pH 4, another ... | [
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PMID:18126 | Gamma glutamyl transpeptidase in colon cancer induced by 1,2-dimethylhydrazine. | The study of the gamma-glutamyl transpeptidase (GTase) in colon of adult rats showed that in the sequence: duodenum, jejunum, ileum, cecum and colon, the colon has the lowest activity. There are, on the other hand, relatively small differences between GTase activities in the ascending, transverse and descending portion... | Gamma glutamyl transpeptidase in colon cancer induced by 1,2-dimethylhydrazine. The study of the gamma-glutamyl transpeptidase (GTase) in colon of adult rats showed that in the sequence: duodenum, jejunum, ileum, cecum and colon, the colon has the lowest activity. There are, on the other hand, relatively small differen... | [
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PMID:18117 | [A study on the behaviour of 7 serological parameters in many subjects affected by acute viral hepatitis (author's transl)]. | The AA. have studied the behaviour of 7 serological parameters in 32 subjects affected by acute viral hapatitis, by means of 4 samples of blood performes at the distance of 12 days for the first and of 15 days for the others. The results are alike to these formerly knows, exception made for alkaline phosphatase, gamma ... | [A study on the behaviour of 7 serological parameters in many subjects affected by acute viral hepatitis (author's transl)]. The AA. have studied the behaviour of 7 serological parameters in 32 subjects affected by acute viral hapatitis, by means of 4 samples of blood performes at the distance of 12 days for the first ... | [
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PMID:18118 | [Quantitative study and typization of aerobic bacteria in the conjunctival sacs of healthy eyes]. | We carried out a qualitative and quantitative bacteriological research into the conjunctives of 220 subjects in good health mostly women (180) exempt from recent or previous eyes diseases. In order to gather conjunctival bacteria we employed with a few variations Hadley's et al. (1973 [12]) method. Among the calculated... | [Quantitative study and typization of aerobic bacteria in the conjunctival sacs of healthy eyes]. We carried out a qualitative and quantitative bacteriological research into the conjunctives of 220 subjects in good health mostly women (180) exempt from recent or previous eyes diseases. In order to gather conjunctival b... | [
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PMID:18127 | Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks. | A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH groups, NAD or NADP as coenzymes, and had an optimum at about pH 7-8. The enzyme's activity against 17 different mo... | Isolation and properties of muscular glucose dehydrogenase (E.C. 1.1.1.47) from the stomachs of geese and ducks. A method which purified 30-fold perparations of muscular glucose dehydrogenase from the stomachs of geese and ducks was elaborated. Activity of this enzyme was found to be dependent on presence of free-SH gr... | [
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PMID:18128 | Analgesic drugs in the management of pain. | The use of potent narcotics to control severe pain should be of short duration and limited to patients with acute diseases or inoperable or metastatic cancer who require long-term relief. Continued and prolonged use of narcotics in patients with chronic benign pain is not recommended because of serious behavioral conse... | Analgesic drugs in the management of pain. The use of potent narcotics to control severe pain should be of short duration and limited to patients with acute diseases or inoperable or metastatic cancer who require long-term relief. Continued and prolonged use of narcotics in patients with chronic benign pain is not reco... | [
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PMID:18129 | A sensitive method for gas-chromatographic assay of barbiturates in body fluids. | The Authors present a gas-chromatographic method which allows for the rapid identification and assay of barbiturates and antiepileptics in body fluids. The Kupferberger procedure for extraction is used. The identification is obtained on two different columns: 10% Dexil 300 GC on Chromosorb W-HP and 3% OV 17 on Chromoso... | A sensitive method for gas-chromatographic assay of barbiturates in body fluids. The Authors present a gas-chromatographic method which allows for the rapid identification and assay of barbiturates and antiepileptics in body fluids. The Kupferberger procedure for extraction is used. The identification is obtained on tw... | [
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PMID:18130 | Spectral evidence for 2,2,3-trichloro-oxirane formation during microsomal trichloroethylene oxidation. | During aerobic incubation of trichloroethylene with rabbit liver microsomes and NADPH a difference absorption peak appears at 451-452 nm. Trichloroethylene does not form a ligand absorption spectrum with hepatic microsomes reduced by dithionite, or in anaerobic incubates in the presence of NADPH. Addition of trichloroe... | Spectral evidence for 2,2,3-trichloro-oxirane formation during microsomal trichloroethylene oxidation. During aerobic incubation of trichloroethylene with rabbit liver microsomes and NADPH a difference absorption peak appears at 451-452 nm. Trichloroethylene does not form a ligand absorption spectrum with hepatic micro... | [
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PMID:18132 | Inheritance of muscle and liver types of supernatant NADP-dependent isocitrate dehydrogenase in rainbow trout (Salmo gairdneri). | The genetics of allelic variation for NADP-dependent isocitrate dehydrogenase (IDH-s) found in the supernatant of liver and white muscle extracts of rainbow trout (Salmo gairdneri) was examined. Twenty progeny from each of 50 controlled matings were examined for IDH phenotypes. Progeny data clearly indicated that the I... | Inheritance of muscle and liver types of supernatant NADP-dependent isocitrate dehydrogenase in rainbow trout (Salmo gairdneri). The genetics of allelic variation for NADP-dependent isocitrate dehydrogenase (IDH-s) found in the supernatant of liver and white muscle extracts of rainbow trout (Salmo gairdneri) was examin... | [
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PMID:18133 | Purification and properties of a new testosterone 17beta-dehydrogenase (NADP+) from guinea-pig liver. | As a result of studies of guinea-pig live testosterone 17beta-dehydrogenase (NADP+) (EC 1.1.1.64), a new testosterone 17beta-dehydrogenase was discovered. The new enzyme was purified to a single homogeneous protein from the 105 000 g-supernatant fraction of guinea-pig liver by (NH4)2SO4 fractional precipitation and two... | Purification and properties of a new testosterone 17beta-dehydrogenase (NADP+) from guinea-pig liver. As a result of studies of guinea-pig live testosterone 17beta-dehydrogenase (NADP+) (EC 1.1.1.64), a new testosterone 17beta-dehydrogenase was discovered. The new enzyme was purified to a single homogeneous protein fro... | [
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PMID:18134 | Partial purification and properties of frog liver tyrosine aminotransferase. | Hepatic tyrosine aminotransferase of the frog Rana temporaria was partially purified by (NH4)2SO4 fractionation and successive chromatography on DEAE-cellulose DE-52, Ultrogel AcA-34, DEAE-cellulose DE-52 again and, finally, hydroxyapatite. During the last step, the enzyme activity separated into two fractions; traces ... | Partial purification and properties of frog liver tyrosine aminotransferase. Hepatic tyrosine aminotransferase of the frog Rana temporaria was partially purified by (NH4)2SO4 fractionation and successive chromatography on DEAE-cellulose DE-52, Ultrogel AcA-34, DEAE-cellulose DE-52 again and, finally, hydroxyapatite. Du... | [
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PMID:18135 | Characterization of an adenosine 3':5'-cyclic monophosphate phosphodiesterase from baker's yeast. Its binding to subcellular particles, catalytic properties and gel-filtration behaviour. | 1. The 3':5'-cyclic AMP phosphodiesterase in the microsomal fraction of baker's yeast is highly specific for cyclic AMP, and not inhibited by cyclic GMP, cyclic IMP or cyclic UMP. Catalytic activity is abolished by 30 micrometer-EDTA. At 30 degrees C and pH8.1, the Km is 0.17 micrometer, and theophylline is a simple co... | Characterization of an adenosine 3':5'-cyclic monophosphate phosphodiesterase from baker's yeast. Its binding to subcellular particles, catalytic properties and gel-filtration behaviour. 1. The 3':5'-cyclic AMP phosphodiesterase in the microsomal fraction of baker's yeast is highly specific for cyclic AMP, and not inhi... | [
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PMID:18136 | Physical and binding properties of large fragments of human serum albumin. | Three large fragments of human serum albumin were produced by peptic digestion of the native protein [Geisow & Beaven (1977) Biochem. J. 161, 619-625]. Fragment P44 represents residues 1-386 and fragments P29 and P31 represent residues 49-307 and residues 308-584 respectively of the albumin molecule. The large N-te... | Physical and binding properties of large fragments of human serum albumin. Three large fragments of human serum albumin were produced by peptic digestion of the native protein [Geisow & Beaven (1977) Biochem. J. 161, 619-625]. Fragment P44 represents residues 1-386 and fragments P29 and P31 represent residues 49-30... | [
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PMID:18137 | Purification and properties of urease from bovine rumen. | Urease (urea amidohydrolase, EC 3.5.1.5) was extracted from the mixed rumen bacterial fraction of bovine rumen contents and purified 60-fold by (NH4)2SO4 precipitation, calcium phosphate-gel adsorption and chromatography on hydroxyapatite. The purified enzyme had maximum activity at pH 8.0. The molecular weight was est... | Purification and properties of urease from bovine rumen. Urease (urea amidohydrolase, EC 3.5.1.5) was extracted from the mixed rumen bacterial fraction of bovine rumen contents and purified 60-fold by (NH4)2SO4 precipitation, calcium phosphate-gel adsorption and chromatography on hydroxyapatite. The purified enzyme had... | [
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PMID:18138 | Adenosine triphosphate-activated adenylate deaminase from marine invertebrate animals. Properties of the enzyme from lugworm (Arenicola cristata) body-wall muscle. | Adenylate deaminase (AMP aminohydrolase, EC 3.5.4.6) from lugworm (Arenicola cristata) body-wall muscle was partially purified by extraction in KCl solutions and chromatography on phosphocellulose. Enzyme activity was eluted from the column at two salt concentrations. Both forms show co-operative binding of AMP (Hill c... | Adenosine triphosphate-activated adenylate deaminase from marine invertebrate animals. Properties of the enzyme from lugworm (Arenicola cristata) body-wall muscle. Adenylate deaminase (AMP aminohydrolase, EC 3.5.4.6) from lugworm (Arenicola cristata) body-wall muscle was partially purified by extraction in KCl solution... | [
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PMID:18139 | Multiple alpha-mannosidase activities in mammalian tissues as shown by metal-ion activation. | 1. Four different types of alpha-mannosidase activity were shown to occur in several tissues from the rat. There is the Zn2+-dependent enzyme, active at acidic pH, and three enzymes that are active near to neutral pH. 2. The 'neutral' enzymes are activated by Fe2+, Co2+ or Mn2+. 3. Optimum activities for these three en... | Multiple alpha-mannosidase activities in mammalian tissues as shown by metal-ion activation. 1. Four different types of alpha-mannosidase activity were shown to occur in several tissues from the rat. There is the Zn2+-dependent enzyme, active at acidic pH, and three enzymes that are active near to neutral pH. 2. The 'n... | [
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PMID:18140 | Specific labelling of a constituent polypeptide of bovine heart mitochondrial reduced nicotinamide-adenine dinucleotide-ubiquinone reductase by the inhibitor diphenyleneiodonium. | 1. NADH-ubiquinone-1 and NADH-menadione reductase activities of Complex I were inhibited by diphenyleneiodonium (apparent Ki 23 and 30 nmol/mg of protein respectively). Reduction of K3Fe(CN)6 and juglone was relatively unaffected. 2. Iodoniumdiphenyl and derivatives were much less effective inhibitors. Compounds with s... | Specific labelling of a constituent polypeptide of bovine heart mitochondrial reduced nicotinamide-adenine dinucleotide-ubiquinone reductase by the inhibitor diphenyleneiodonium. 1. NADH-ubiquinone-1 and NADH-menadione reductase activities of Complex I were inhibited by diphenyleneiodonium (apparent Ki 23 and 30 nmol/m... | [
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PMID:18141 | Changes in the kinetic behaviour of threonine transport into Trypanosoma brucei elicited by variation in hydrogen ion concentration. | 1. The dependence of V and V/K(m) for threonine transport into Trypanosoma brucei upon the external concentration of H(+) was studied. 2. Two ionizing groups, the alpha-amino group of the substrate and a group at the substrate-binding site of the carrier, were found to influence the observed kinetic behaviour of transp... | Changes in the kinetic behaviour of threonine transport into Trypanosoma brucei elicited by variation in hydrogen ion concentration. 1. The dependence of V and V/K(m) for threonine transport into Trypanosoma brucei upon the external concentration of H(+) was studied. 2. Two ionizing groups, the alpha-amino group of the... | [
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PMID:18142 | Effect of prolonged ethanol ingestion on hepatic lipogenesis and related enzyme activities. | 1. Hepatic lipogenesis in vivo and the activities of enzymes associated with fatty acid synthesis in the liver were studied in rats fed for 21 days on liquid diets containing ethanol. 2. The ethanol-fed rats developed a moderate hepatic triacylglycerol accumulation during this period. When carbohydrate was replaced by ... | Effect of prolonged ethanol ingestion on hepatic lipogenesis and related enzyme activities. 1. Hepatic lipogenesis in vivo and the activities of enzymes associated with fatty acid synthesis in the liver were studied in rats fed for 21 days on liquid diets containing ethanol. 2. The ethanol-fed rats developed a moderate... | [
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PMID:18143 | The mechanism of inhibition by acidosis of gluconeogenesis from lactate in rat liver. | 1. Gluconeogenesis from lactate or pyruvate was studied in perfused livers from starved rats at perfusate pH7.4 or under conditions simulating uncompensated metabolic acidosis (perfusate pH6.7-6.8). 2. In 'acidotic' perfusions gluconeogenesis and uptake of lactate or pyruvate were decreased. 3. Measurement of hepatic i... | The mechanism of inhibition by acidosis of gluconeogenesis from lactate in rat liver. 1. Gluconeogenesis from lactate or pyruvate was studied in perfused livers from starved rats at perfusate pH7.4 or under conditions simulating uncompensated metabolic acidosis (perfusate pH6.7-6.8). 2. In 'acidotic' perfusions glucone... | [
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