id stringlengths 7 10 | title stringlengths 8 354 | content stringlengths 91 4.1k | contents stringlengths 164 4.24k | embedding listlengths 384 384 |
|---|---|---|---|---|
PMID:19007 | Activities of some peptidases and glycolytic enzymes in cells of the amniotic fluid. i. Pregnancies terminated prematurely, at term, and postmaturely. | Activities of leucylaminopeptidase, gamma-glutamyl transpeptidase, oxytocinase and aldolase were significantly elevated in the cells of amniotic fluids from cases of premature delivery, compared with cases of delivery at term. The authors conclude that examination of the activities of the aforementioned enzymes can be ... | Activities of some peptidases and glycolytic enzymes in cells of the amniotic fluid. i. Pregnancies terminated prematurely, at term, and postmaturely. Activities of leucylaminopeptidase, gamma-glutamyl transpeptidase, oxytocinase and aldolase were significantly elevated in the cells of amniotic fluids from cases of pre... | [
-0.04893386736512184,
-0.05038866028189659,
-0.030589278787374496,
0.007219518069177866,
0.017786825075745583,
-0.04120529070496559,
0.07919265329837799,
0.03847604617476463,
-0.02545153722167015,
-0.03938642516732216,
-0.016556067392230034,
-0.03991841524839401,
-0.06120043247938156,
0.05... |
PMID:19008 | Variables of the rubella hemagglutination tests employing freeze-dried erythrocytes. | The variables which affect the interaction between freeze-dried one-day-old chick erythrocytes and rubella hemagglutinin prepared from rubella-infected porcine kidney cells were defined and evaluated. The sensitivity of the hemagglutination (HA) reaction is much greater at pH 6.0 to 6.2 than at pH 7.0 to 7.5 HEPES (N-2... | Variables of the rubella hemagglutination tests employing freeze-dried erythrocytes. The variables which affect the interaction between freeze-dried one-day-old chick erythrocytes and rubella hemagglutinin prepared from rubella-infected porcine kidney cells were defined and evaluated. The sensitivity of the hemagglutin... | [
-0.07653085142374039,
0.018940996378660202,
-0.05449709668755531,
0.025447072461247444,
0.04032808542251587,
0.014992237091064453,
0.08625468611717224,
0.06018930301070213,
-0.050763826817274094,
-0.005037413910031319,
-0.03168069198727608,
-0.07168552279472351,
0.022543245926499367,
-0.00... |
PMID:19011 | Purification of rat liver particulate neutral ribonuclease and comparison of properties with pancreas and serum ribonucleases. | Rat liver particulate neutral ribonuclease (EC 3.1.4.22) was extensively purified (up to 40000-fold). It is shown to be an endonuclease, specific for pyrimidine bases, hydrolysing 5'-phosphate ester bonds. The enzyme specificity, Km, pH optimum, stability in acid medium and thermal stability at high temperature are the... | Purification of rat liver particulate neutral ribonuclease and comparison of properties with pancreas and serum ribonucleases. Rat liver particulate neutral ribonuclease (EC 3.1.4.22) was extensively purified (up to 40000-fold). It is shown to be an endonuclease, specific for pyrimidine bases, hydrolysing 5'-phosphate ... | [
-0.06095486134290695,
-0.031615301966667175,
-0.02338315360248089,
-0.017947034910321236,
0.021334035322070122,
-0.0442190058529377,
0.05416104197502136,
0.04923473298549652,
-0.07611328363418579,
-0.0023556742817163467,
0.015294565819203854,
-0.09458895772695541,
0.0077330260537564754,
-0... |
PMID:19012 | Identification and partial characterization of phospholipases in isolated adrenocortical cells. The effects of synacthen [corticotropin-(1--24)-tetracosapeptide] and calcium ions. | Phospholipase A activity was determined in homogenates and subcellular fractions of trypsin-dispersed cat adrenocortical cells. At pH 7.4 homogenate phospholipid hydrolysis was activated by added Ca2+ and inhibited by EGTA. Phospholipid degradation in the presence and absence of Synacthen was completely blocked by EGTA... | Identification and partial characterization of phospholipases in isolated adrenocortical cells. The effects of synacthen [corticotropin-(1--24)-tetracosapeptide] and calcium ions. Phospholipase A activity was determined in homogenates and subcellular fractions of trypsin-dispersed cat adrenocortical cells. At pH 7.4 ho... | [
-0.05534970760345459,
0.008071459829807281,
0.015443215146660805,
0.012933999300003052,
-0.06028536707162857,
-0.028509482741355896,
0.09451638162136078,
0.0202308502048254,
-0.048243600875139236,
-0.00044789555249735713,
0.009649562649428844,
-0.05974113941192627,
-0.011430682614445686,
0... |
PMID:19013 | A pulse-radiolysis study of the manganese-containing superoxide dismutase from Bacillus stearothermophilus. | In the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from Bacillus stearothermophilus was shown to involve a 'fast cycle' and a 'slow cycle' [McAdam, Fox, Lavelle & Fielden, 1977 (Biochem. J. 165, 71-79)]. Further properties of the enzyme was considered in the present p... | A pulse-radiolysis study of the manganese-containing superoxide dismutase from Bacillus stearothermophilus. In the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from Bacillus stearothermophilus was shown to involve a 'fast cycle' and a 'slow cycle' [McAdam, Fox, Lavelle &am... | [
-0.07533597201108932,
0.04489724338054657,
-0.06219145283102989,
0.020984522998332977,
0.08039204776287079,
-0.06159019470214844,
0.018363289535045624,
0.020141392946243286,
-0.07851298898458481,
-0.06073455512523651,
0.0035626976750791073,
-0.06519754976034164,
0.0424729585647583,
0.06006... |
PMID:19014 | Purification and some properties of arylsulphatases A and B from rabbit kidney cortex. | Arylsulphatases A and B (EC 3.1.6.1) of rabbit kidney cortex were purified 5250- and 7720-fold respectively by a multiple-column-chromatography method. The specific activity toward 4-nitrocatechol sulphate was 42mumol/min per mg for arylsulphatase A and 62 mumol/min per mg for arylsulphatase B. Each enzyme migrated as... | Purification and some properties of arylsulphatases A and B from rabbit kidney cortex. Arylsulphatases A and B (EC 3.1.6.1) of rabbit kidney cortex were purified 5250- and 7720-fold respectively by a multiple-column-chromatography method. The specific activity toward 4-nitrocatechol sulphate was 42mumol/min per mg for... | [
-0.06702520698308945,
-0.015117132104933262,
-0.062075771391391754,
0.021929193288087845,
-0.012495440430939198,
-0.010149193927645683,
0.07478136569261551,
0.010168233886361122,
-0.011305774562060833,
0.014590258710086346,
-0.0008907989831641316,
-0.09417881071567535,
0.012487191706895828,
... |
PMID:19015 | Purification and properties of a cellulase from Aspergillus niger. | A cellulolytic enzyme was isolated from a commercial cellulase preparation form Aspergillus niger. A yield of about 50mg of enzyme was obtained per 100g of commerial cellulase. The isolated enzyme was homogeneous in the ultracentrifuge at pH 4.0 and 8.0, and in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis... | Purification and properties of a cellulase from Aspergillus niger. A cellulolytic enzyme was isolated from a commercial cellulase preparation form Aspergillus niger. A yield of about 50mg of enzyme was obtained per 100g of commerial cellulase. The isolated enzyme was homogeneous in the ultracentrifuge at pH 4.0 and 8.0... | [
-0.06226982921361923,
0.027667595073580742,
-0.05512653663754463,
-0.02008032612502575,
0.004858414642512798,
-0.02996622398495674,
0.02006066031754017,
-0.0006865134346298873,
-0.04786596819758415,
0.03498535230755806,
0.009942013770341873,
-0.061173900961875916,
-0.05276571214199066,
0.0... |
PMID:19025 | Respiratory effects and amnesia after premedication with morphine or lorazepam. | Lorazepam, a new benzodiazepine, was compared with morphine for premedication. Ten patients received morphine 10 mg/70 kg i.m. and 10 received lorazepam 4 mg/70 kg i.m. Respiratory effects were assessed from the change in slope (S) and intercept (B) of the carbon dioxide response line, using a development of Read's reb... | Respiratory effects and amnesia after premedication with morphine or lorazepam. Lorazepam, a new benzodiazepine, was compared with morphine for premedication. Ten patients received morphine 10 mg/70 kg i.m. and 10 received lorazepam 4 mg/70 kg i.m. Respiratory effects were assessed from the change in slope (S) and inte... | [
-0.04016469419002533,
0.011132624931633472,
0.024636749178171158,
0.05485932156443596,
0.017611272633075714,
-0.004453815519809723,
0.024486960843205452,
-0.011767463758587837,
-0.010840033181011677,
-0.04442859813570976,
-0.026234297081828117,
0.06046300753951073,
0.004579926375299692,
0.... |
PMID:19027 | Correlation of biochemical data with Apgar scores at birth and at one minute. | A prospective study of 66 unselected neonates revealed a better correlation of umbilical artery blood biochemical data with the Apgar score at birth compared with the score at 1 min. The data confirmed also that inclusion of the score for "colour" detracts from the value of the total score. An Apgar score at birth is m... | Correlation of biochemical data with Apgar scores at birth and at one minute. A prospective study of 66 unselected neonates revealed a better correlation of umbilical artery blood biochemical data with the Apgar score at birth compared with the score at 1 min. The data confirmed also that inclusion of the score for "co... | [
-0.03376813605427742,
0.023660751059651375,
-0.002018977189436555,
0.002390848472714424,
0.03259376809000969,
0.008613863959908485,
0.05127530172467232,
0.023059621453285217,
0.05231326445937157,
0.003703507361933589,
0.020134346559643745,
-0.04282138869166374,
0.0028094719164073467,
0.040... |
PMID:19028 | Cancer of the oral cavity, pharynx/larynx and lung in North Thailand: case-control study and analysis of cigar smoke. | The unusually high relative frequency of cancer in the laryngeal region in males (18% of all histologically diagnosed cancers) and a sex ratio of unity for lung cancer in Northern Thailand were further explored in a hospital-based case-control study in Chiang Mai. This compared patients having cancers of the oral cavit... | Cancer of the oral cavity, pharynx/larynx and lung in North Thailand: case-control study and analysis of cigar smoke. The unusually high relative frequency of cancer in the laryngeal region in males (18% of all histologically diagnosed cancers) and a sex ratio of unity for lung cancer in Northern Thailand were further ... | [
0.006857919041067362,
0.028332509100437164,
0.015311971306800842,
-0.02247844636440277,
0.0495016947388649,
0.007283356506377459,
0.10375329852104187,
-0.01098130363970995,
-0.03544469550251961,
-0.009133844636380672,
-0.049254052340984344,
-0.0911785364151001,
0.016298538073897362,
0.0440... |
PMID:19029 | Blocking factors against leucocyte-dependent melanoma antibody in the sera of melanoma patients. | Previous studies, using plasmapheresis to remove blocking factors of cell-mediated cytotoxicity to melanoma cells from the circulation of melanoma patients, suggested that leucocyte-dependent antibody to melanoma cells may also be blocked by factors in their sera. The present study confirms these findings, by showing t... | Blocking factors against leucocyte-dependent melanoma antibody in the sera of melanoma patients. Previous studies, using plasmapheresis to remove blocking factors of cell-mediated cytotoxicity to melanoma cells from the circulation of melanoma patients, suggested that leucocyte-dependent antibody to melanoma cells may ... | [
-0.08933993428945541,
0.007647986989468336,
-0.020962197333574295,
0.022710386663675308,
0.0343918576836586,
-0.018034758046269417,
0.1280517876148224,
0.06730666756629944,
-0.039523299783468246,
0.01598067209124565,
0.01579922065138817,
-0.03530149906873703,
-0.015609046444296837,
-0.0102... |
PMID:19030 | Serum ferritin in haemochromatosis: changes in the isoferritin composition during venesection therapy. | The isoferritin composition of serum ferritin in 13 patients with untreated idiopathic haemochromatosis (IHC) has been shown to differ from normal in exhibiting an increase in isoferritins in the pH range 5.54-5.62. A similar change was observed in four patients with gross iron overload secondary to haemolytic anaemia.... | Serum ferritin in haemochromatosis: changes in the isoferritin composition during venesection therapy. The isoferritin composition of serum ferritin in 13 patients with untreated idiopathic haemochromatosis (IHC) has been shown to differ from normal in exhibiting an increase in isoferritins in the pH range 5.54-5.62. A... | [
-0.05809784680604935,
0.009422753937542439,
-0.027852598577737808,
0.028347600251436234,
-0.013855150900781155,
0.005910247564315796,
0.038910750299692154,
0.0753186047077179,
-0.07919744402170181,
-0.04197614639997482,
0.06545329838991165,
-0.07963091135025024,
0.0009555655415169895,
0.05... |
PMID:19031 | Decreased deformability of erythrocytes in haemolytic anaemia associated with glucosephosphate isomerase deficiency. | Deformability of erythrocytes from four patients with different types of glucosephosphate isomerase (D-glucose-6-phosphate ketoisomerase, GPI) deficiency has been determined by cell filtration. Young as well as whole erythrocyte populations had a markedly increased rigidity and an abnormally strong attachment of haemog... | Decreased deformability of erythrocytes in haemolytic anaemia associated with glucosephosphate isomerase deficiency. Deformability of erythrocytes from four patients with different types of glucosephosphate isomerase (D-glucose-6-phosphate ketoisomerase, GPI) deficiency has been determined by cell filtration. Young as ... | [
-0.06305854767560959,
0.010479277931153774,
0.014962607063353062,
0.05358166992664337,
-0.05865106359124184,
0.011118822731077671,
0.038760896772146225,
0.08475913852453232,
-0.10861033946275711,
0.021342288702726364,
0.027408068999648094,
-0.06465867906808853,
-0.045303624123334885,
0.049... |
PMID:19032 | A to O bone marrow transplantation in severe aplastic anaemia: dynamics of blood group conversion and demonstration of early dyserythropoiesis in the engrafted marrow. | A to O bone marrow transplantation was performed in a 25-year-old male affected with severe aplastic anaemia, the donor being an HLA compatible brother. Three plasma exchanges had to be performed with an Aminco separator to remove the original and recurring anti-A isohaemagglutinins. The dynamics of O to A blood group ... | A to O bone marrow transplantation in severe aplastic anaemia: dynamics of blood group conversion and demonstration of early dyserythropoiesis in the engrafted marrow. A to O bone marrow transplantation was performed in a 25-year-old male affected with severe aplastic anaemia, the donor being an HLA compatible brother.... | [
-0.0660983994603157,
0.021376565098762512,
-0.01676945760846138,
-0.006775469519197941,
-0.06014406308531761,
-0.015835754573345184,
0.021971387788653374,
0.03906497359275818,
-0.09109542518854141,
-0.03034197725355625,
-0.008412820287048817,
-0.04420284181833267,
-0.017044829204678535,
-0... |
PMID:19033 | Reciprocal interaction of hemoglobin with oxygen and protons. The influence of allosteric polyanions. | The interaction of three inositol esters, inositol hexaphosphate (IHP), inositol pentaphosphate (IPP), and inositol hexasulfate (IHS), with hemoglobin has been investigated. The proton uptake method was used to obtain the six binding constants for deoxy- and oxyhemoglobin. These data combined with oxygen binding curves... | Reciprocal interaction of hemoglobin with oxygen and protons. The influence of allosteric polyanions. The interaction of three inositol esters, inositol hexaphosphate (IHP), inositol pentaphosphate (IPP), and inositol hexasulfate (IHS), with hemoglobin has been investigated. The proton uptake method was used to obtain ... | [
-0.09439143538475037,
0.06051255762577057,
-0.03879924491047859,
0.001950776786543429,
0.0023649639915674925,
-0.036955151706933975,
0.09082550555467606,
0.033126238733530045,
-0.06609266251325607,
-0.0376066230237484,
0.03427332267165184,
-0.06096560135483742,
-0.012222396209836006,
-0.01... |
PMID:19034 | Conformations of synthetic tetradecapeptide renin substrate and of angiotensin I in aqueous solution. | The properties of aqueous solutions of synthetic renin substrate tetradecapeptide (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser) were examined through electrometric titrations, infrared and circular dichroism spectroscopy, and spectrofluorometry. Titration studies of angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pr... | Conformations of synthetic tetradecapeptide renin substrate and of angiotensin I in aqueous solution. The properties of aqueous solutions of synthetic renin substrate tetradecapeptide (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser) were examined through electrometric titrations, infrared and circular dichrois... | [
-0.10288071632385254,
-0.00016891864652279764,
0.00877164863049984,
-0.0078309066593647,
-0.0006239438662305474,
-0.016572339460253716,
0.04872795194387436,
-0.0050496505573391914,
-0.0306108221411705,
-0.03740336373448372,
-0.0014827164122834802,
-0.049225132912397385,
0.032326918095350266,... |
PMID:19036 | Effects of quaternary ligands on the inhibition of acetylcholinesterase by arsenite. | Arsenite inhibits acetylcholinesterase in a second-order reaction. The rate and equilibrium constants depend upon pH and have values on the order of 10(2) M-1 min-1 and 10(5) M (dissociation), respectively. Some quaternary ammonium ligands completely block the arsenite inhibition of the enzyme, others decrease the rate... | Effects of quaternary ligands on the inhibition of acetylcholinesterase by arsenite. Arsenite inhibits acetylcholinesterase in a second-order reaction. The rate and equilibrium constants depend upon pH and have values on the order of 10(2) M-1 min-1 and 10(5) M (dissociation), respectively. Some quaternary ammonium lig... | [
-0.09310778230428696,
0.04015450179576874,
-0.06764402985572815,
0.052439700812101364,
0.021326415240764618,
-0.014658884145319462,
0.0360831581056118,
0.036465417593717575,
-0.05321643501520157,
-0.04608917981386185,
0.04074229300022125,
-0.016095027327537537,
0.011510089039802551,
0.0749... |
PMID:19038 | Redox properties of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase and cytochrome b5. | Hepatic NADH-cytochrome b5 reductase was reduced by 1 mol of dithionite or NADH per mol of enzyme-bound FAD, without forming a stable semiquinone or intermediate during the titrations. However, the addition of NAD+ to the partially reduced enzyme or illumination in the presence of both NAD+ and EDTA yielded a new inter... | Redox properties of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase and cytochrome b5. Hepatic NADH-cytochrome b5 reductase was reduced by 1 mol of dithionite or NADH per mol of enzyme-bound FAD, without forming a stable semiquinone or intermediate during the titrations. However, the additi... | [
-0.09889309108257294,
0.03379799425601959,
-0.04632331803441048,
0.05933781713247299,
0.030868131667375565,
0.01761244796216488,
0.019800031557679176,
0.054979413747787476,
-0.03749080002307892,
-0.03713328763842583,
0.01819516159594059,
-0.06699302792549133,
0.007458690088242292,
0.050183... |
PMID:19039 | Phosphoglycerate mutase from wheat germ: studies with isotopically labeled 3-phospho-D-glycerates showing that the catalyzed reaction is intramolecular. Appendix: phosphoglycerate mutase from wheat germ: isolation, crystallization, and properties. | The isomerization of 3-phospho-D-glycerate and 2-phospho-D-glycerate catalyzed by the cofactor-independent phosphoglycerate mutase from wheat germ (the isolation and crystallization of which is described in the Appendix) has been shown to be intramolecular by two methods. Mass-spectrometric analysis of the products fro... | Phosphoglycerate mutase from wheat germ: studies with isotopically labeled 3-phospho-D-glycerates showing that the catalyzed reaction is intramolecular. Appendix: phosphoglycerate mutase from wheat germ: isolation, crystallization, and properties. The isomerization of 3-phospho-D-glycerate and 2-phospho-D-glycerate cat... | [
-0.011739829555153847,
0.0020798067562282085,
0.01619812101125717,
-0.005555222742259502,
0.0038703158497810364,
-0.0721302255988121,
-0.014217060059309006,
-0.006221721414476633,
-0.09960979968309402,
0.008078590966761112,
0.04451373219490051,
-0.10848076641559601,
-0.024028465151786804,
... |
PMID:19042 | Preparation and properties of a new DNase from Aspergillus oryzae. | A DNase present in commercial preparations of Aspergillus oryzae alpha-amylase was purified 1550-fold in 25% yield by acetone precipitation and by chromatography on diethylaminoethyl- and carboxymethylcellulose. The enzyme was isolated free of contaminating RNases and DNases. The molecular weight of the enzyme determin... | Preparation and properties of a new DNase from Aspergillus oryzae. A DNase present in commercial preparations of Aspergillus oryzae alpha-amylase was purified 1550-fold in 25% yield by acetone precipitation and by chromatography on diethylaminoethyl- and carboxymethylcellulose. The enzyme was isolated free of contamina... | [
-0.043436937034130096,
-0.0072175199165940285,
-0.04271877557039261,
0.02727873995900154,
-0.0013905251398682594,
-0.025405999273061752,
0.025939565151929855,
0.055850617587566376,
-0.051071833819150925,
0.016964834183454514,
0.02214984968304634,
-0.03598349168896675,
-0.07721497118473053,
... |
PMID:19044 | Structure-function relationships in TPN-dependent isocitrate dehydrogenase. I. Electron paramagnetic resonance studies of the interaction of enzyme-bound Mn(II) with substrates, cofactors, and substrate analogues. | Electron paramagnetic resonance (EPR) spectra were obtained for various isocitrate dehydrogenase-Mn(II) complexes. The qualitative effects of the binding of substrates, nucleotides, and substrate analogues on the isotropic character of the electronic environment of enzyme-bound Mn(II) were subsequently investigated. Th... | Structure-function relationships in TPN-dependent isocitrate dehydrogenase. I. Electron paramagnetic resonance studies of the interaction of enzyme-bound Mn(II) with substrates, cofactors, and substrate analogues. Electron paramagnetic resonance (EPR) spectra were obtained for various isocitrate dehydrogenase-Mn(II) co... | [
-0.09862040728330612,
0.06368231028318405,
-0.05789065361022949,
0.04470810294151306,
0.03882020711898804,
-0.020586755126714706,
0.07074318081140518,
0.03440803661942482,
-0.0760132297873497,
-0.04056140407919884,
0.053943436592817307,
-0.019540056586265564,
0.02713100239634514,
0.0259457... |
PMID:19046 | Purification and properties of a 3'-phosphoryl former endodeoxyribonuclease from eggs of Asterias forbesi. | A DNA endonuclease has been purified from eggs of Asterias forbesi by a simple four-step-purification procedure. The purified enzyme is at least 96% pure and is free of phosphatase, phosphodiesterase, and RNase. It has a pH optimum of 6.5 and does not require divalent cations. The enzyme produces 3'-phosphoryl and 5'-h... | Purification and properties of a 3'-phosphoryl former endodeoxyribonuclease from eggs of Asterias forbesi. A DNA endonuclease has been purified from eggs of Asterias forbesi by a simple four-step-purification procedure. The purified enzyme is at least 96% pure and is free of phosphatase, phosphodiesterase, and RNase. I... | [
-0.02600841596722603,
-0.032570548355579376,
-0.04548707976937294,
0.0014301353367045522,
0.010998042300343513,
-0.043497417122125626,
0.028744643554091454,
0.037863776087760925,
-0.04395536705851555,
-0.008848645724356174,
0.0384172722697258,
-0.055619001388549805,
-0.02189479023218155,
0... |
PMID:19047 | Effect of pH on the interaction of benzoate and D-amino acid oxidase. | The kinetic and equilibrium dissociation constants of the reversible binding of benzoate to hog kidney D-amino acid oxidase (DAAO) were studied at 19 degrees C over the pH range 5.3-10.5 by means of a stopped-flow apparatus and spectrophotometric titrations. A simple bimolecular reaction of the form second order-first ... | Effect of pH on the interaction of benzoate and D-amino acid oxidase. The kinetic and equilibrium dissociation constants of the reversible binding of benzoate to hog kidney D-amino acid oxidase (DAAO) were studied at 19 degrees C over the pH range 5.3-10.5 by means of a stopped-flow apparatus and spectrophotometric tit... | [
-0.10962232947349548,
0.02670983225107193,
-0.042472101747989655,
0.0066807447001338005,
0.04088288173079491,
-0.042384348809719086,
0.0465644896030426,
0.00004922288644593209,
-0.04627174139022827,
-0.04005108028650284,
0.03517911210656166,
-0.09021332114934921,
0.029960142448544502,
0.03... |
PMID:19053 | Pre-steady-state kinetics of intermediate formation in the deuteroferriheme-hydrogen peroxide system. | The pH dependence of formation of a peroxidatic intermediate from the reaction of deuteroferriheme with hydrogen peroxide has been determined for the region pH 8.7-10.1 from stopped-flow kinetic studies in which absorbancy changes are observed at heme monomer-dimer isosbestic points. Results are interpreted primarily i... | Pre-steady-state kinetics of intermediate formation in the deuteroferriheme-hydrogen peroxide system. The pH dependence of formation of a peroxidatic intermediate from the reaction of deuteroferriheme with hydrogen peroxide has been determined for the region pH 8.7-10.1 from stopped-flow kinetic studies in which absorb... | [
-0.09743048250675201,
0.06217709928750992,
-0.013058136217296124,
0.01566166803240776,
0.058269042521715164,
-0.05383198335766792,
0.000963329803198576,
0.02441813424229622,
-0.017752064391970634,
-0.004368306137621403,
0.014970320276916027,
-0.07173223793506622,
0.031012821942567825,
0.03... |
PMID:19054 | Escherichia coli dihydrofolate reductase: isolation and characterization of two isozymes. | A combination of affinity column chromatography and preparative gel electrophoresis has been used to purify to homogeneity the two isozymes of dihydrofolate reductase from a trimethoprim-resistant strain of Escherichia coli B (RT 500). These enzyme forms are noninterconvertible and are present in crude cell lysates, bu... | Escherichia coli dihydrofolate reductase: isolation and characterization of two isozymes. A combination of affinity column chromatography and preparative gel electrophoresis has been used to purify to homogeneity the two isozymes of dihydrofolate reductase from a trimethoprim-resistant strain of Escherichia coli B (RT ... | [
-0.10398416966199875,
-0.026628758758306503,
-0.09320339560508728,
0.025494763627648354,
0.0222475528717041,
-0.009560707025229931,
0.010561589151620865,
0.06916730850934982,
-0.03052815981209278,
-0.003545027691870928,
0.0096751032397151,
-0.07105489820241928,
0.02674456499516964,
-0.0367... |
PMID:19055 | Thermal unfolding transition of ribonuclease A measured by 2'-CMP binding. | We report an approach to the problem of detecting and characterising intermediates in the unfolding of ribonuclease A. Two distinct properties of the protein are compared at equilibrium within the unfolding transition zone: (1) a physical property of the protein, the absorbance of buried tyrosine residues, and (2) a fu... | Thermal unfolding transition of ribonuclease A measured by 2'-CMP binding. We report an approach to the problem of detecting and characterising intermediates in the unfolding of ribonuclease A. Two distinct properties of the protein are compared at equilibrium within the unfolding transition zone: (1) a physical proper... | [
-0.11576958000659943,
-0.003947688266634941,
-0.03555908799171448,
0.01930380053818226,
0.005383081268519163,
-0.013395639136433601,
0.044153761118650436,
0.0060583013109862804,
-0.03369548171758652,
0.004715802613645792,
0.04686032980680466,
-0.060510482639074326,
0.003802910214290023,
-0... |
PMID:19056 | Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos. | An N2-guanine RNA methyltransferase has been purified 1000-fold from chick embryo homogenates by phosphocellulose chromatography followed by chromatography on S-adenosylhomocystein-Sepharose. The enzyme was shown to methylate the G10 position of Escherichia coli B tRNAPhe and has a Km of 3X10(-7) M for tRNAPhe and 1.38... | Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos. An N2-guanine RNA methyltransferase has been purified 1000-fold from chick embryo homogenates by phosphocellulose chromatography followed by chromatography on S-adenosylhomocystein-Sepharose. The enzyme was shown to m... | [
-0.03816813603043556,
-0.01824808493256569,
-0.09244515001773834,
-0.02717239037156105,
-0.0036650567781180143,
0.05494948476552963,
-0.0005193051183596253,
0.03585568070411682,
-0.01654300093650818,
0.03196537494659424,
-0.0655641034245491,
-0.08247994631528854,
0.0012774020433425903,
-0.... |
PMID:19063 | Mechanism of pigeon liver malic enzyme modification of histidyl residues by ethoxyformic anhydride. | Incubation of malic enzyme (L-malate:NADP+ oxidoreductase (oxaloacetate-decarboxylating), EC 1.1.1.40) with ethoxyformic anhydride caused the time-dependent loss of its ability to catalyze reactions requiring the nucleotide cofactor NADP+ or NADPH, such as the oxidative decarboxylase, the NADP+ - stimualted oxalacetate... | Mechanism of pigeon liver malic enzyme modification of histidyl residues by ethoxyformic anhydride. Incubation of malic enzyme (L-malate:NADP+ oxidoreductase (oxaloacetate-decarboxylating), EC 1.1.1.40) with ethoxyformic anhydride caused the time-dependent loss of its ability to catalyze reactions requiring the nucleot... | [
-0.07584293186664581,
-0.04370373487472534,
0.03575601428747177,
0.03940744698047638,
0.04304821416735649,
0.012175620533525944,
0.04612823203206062,
0.025834906846284866,
-0.08839719742536545,
-0.04667472839355469,
0.057085491716861725,
-0.036625321954488754,
0.04042499512434006,
0.014998... |
PMID:19064 | Characterization of human platelet UDPglucose-collagen glucosyltransferase using a new rapid assay. | A rapid and specific assay has been developed for UDPglucose-collagen glucosyltransferase (UDPglucose: 5-hydroxylysine-collagen glucosyltransferase, EC 2.4.1.66) using galactosylhydroxylysine (Gal-Hyl) as acceptor. Studies with intact human platelets and isolated plasma membranes indicated that about 5--10% of the tota... | Characterization of human platelet UDPglucose-collagen glucosyltransferase using a new rapid assay. A rapid and specific assay has been developed for UDPglucose-collagen glucosyltransferase (UDPglucose: 5-hydroxylysine-collagen glucosyltransferase, EC 2.4.1.66) using galactosylhydroxylysine (Gal-Hyl) as acceptor. Studi... | [
-0.08218878507614136,
-0.056936606764793396,
-0.015218405053019524,
-0.005503186024725437,
0.012146192602813244,
-0.013538733124732971,
0.0014659258304163814,
0.062454916536808014,
-0.028982458636164665,
-0.05010456219315529,
0.02834954299032688,
-0.10244959592819214,
-0.024852849543094635,
... |
PMID:19066 | Cyclic AMP-dependent protein kinase phosphorylation of cardiac (Na+ + K+)-ATPases. Effect on calcium binding. | 1. Calcium binding to (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) preparations from beef and pig heart preparations of varying degrees of purity was measured. 2. Binding was inhibited by Mg2+, Na+ and K+. Inhibition by Na+ and K+ appeared to be due to an ionic strength effect. 3. Four classes of binding sites ... | Cyclic AMP-dependent protein kinase phosphorylation of cardiac (Na+ + K+)-ATPases. Effect on calcium binding. 1. Calcium binding to (Na+ + K+)-ATPase (ATP phosphohydrolase, EC 3.6.1.3) preparations from beef and pig heart preparations of varying degrees of purity was measured. 2. Binding was inhibited by Mg2+, Na+ and ... | [
-0.06611200422048569,
-0.015124357305467129,
-0.005731064826250076,
0.024806411936879158,
0.009147098287940025,
-0.06637585908174515,
0.04895676672458649,
0.017535706982016563,
-0.035687848925590515,
-0.033981870859861374,
0.06515403836965561,
-0.053588684648275375,
-0.01070194598287344,
0... |
PMID:19067 | 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase. An optimized assay in homogenates of developing brain. | An optimized in vitro assay of 3'-phosphoadenylysulfate:galactosylceramide 3'-sulfotransferase (EC 2.8.2.11, galactosylceramide sulfotransferase, formerly known as galactocerebroside sulfotransferase) activity is presented, that can be used in crude homogenate of brain tissue of various developmental stages. The enzyme... | 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase. An optimized assay in homogenates of developing brain. An optimized in vitro assay of 3'-phosphoadenylysulfate:galactosylceramide 3'-sulfotransferase (EC 2.8.2.11, galactosylceramide sulfotransferase, formerly known as galactocerebroside sulfotransferase... | [
0.007012383546680212,
-0.028038248419761658,
-0.03368332237005234,
-0.012214419431984425,
-0.02775094285607338,
-0.005027578677982092,
0.013928703032433987,
0.022150631994009018,
-0.055234551429748535,
-0.018348967656493187,
0.07455775141716003,
-0.12563250958919525,
-0.01650252379477024,
... |
PMID:19068 | Spontaneous reactivation of acetylcholinesterase following organophosphate inhibition. I. An analysis of anomalous reactivation kinetics. | The first kinetic studies on the spontaneous reactivation of Sarin-inhibited acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) are reported. With increasing pH the extent of reactivation increases while the observed rate constant decreases. An analysis of the change in aging rate constant as a function of pH s... | Spontaneous reactivation of acetylcholinesterase following organophosphate inhibition. I. An analysis of anomalous reactivation kinetics. The first kinetic studies on the spontaneous reactivation of Sarin-inhibited acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) are reported. With increasing pH the extent of... | [
-0.09404592961072922,
0.001344995922408998,
-0.024280570447444916,
0.020971404388546944,
-0.02975994348526001,
-0.04423175007104874,
0.05971154198050499,
0.022033413872122765,
-0.04503666236996651,
-0.08649004250764847,
0.02857407182455063,
0.009355590678751469,
-0.02519909106194973,
0.061... |
PMID:19069 | Comparative studies on immobilization of human prostatic acid phosphatase. | Acid phosphatase (othophosphoric monoester phosphohydrolase (acid optimum), EC 3.1.3.2) from the human prostate was immobilized by its protein moiety on cyanogen bromide-activated Sepharose, by carbohydrate moiety on Concanavalin-A-Sepharose, and by Schiff base formation with partially oxidized carbohydrate groups on e... | Comparative studies on immobilization of human prostatic acid phosphatase. Acid phosphatase (othophosphoric monoester phosphohydrolase (acid optimum), EC 3.1.3.2) from the human prostate was immobilized by its protein moiety on cyanogen bromide-activated Sepharose, by carbohydrate moiety on Concanavalin-A-Sepharose, an... | [
-0.08848747611045837,
0.0018161829793825746,
-0.061498261988162994,
-0.01387841161340475,
-0.028729738667607307,
-0.043679554015398026,
0.06287843734025955,
0.06170323118567467,
-0.07129579037427902,
-0.03580066189169884,
0.06452732533216476,
-0.07307218760251999,
-0.03855717554688454,
0.0... |
PMID:19070 | Harmaline interaction with sodium-binding sites in intestinal brush border sucrase. | The effect of harmaline on rabbit brush border sucrase has been studied at pH 6.8. An initial analysis in classical kinetic terms revealed harmaline to be a fully competitive inhibitor of the substrate, sucrose. In spite of this result however, the following hypothesis has been tested. Harmaline, which is positively ch... | Harmaline interaction with sodium-binding sites in intestinal brush border sucrase. The effect of harmaline on rabbit brush border sucrase has been studied at pH 6.8. An initial analysis in classical kinetic terms revealed harmaline to be a fully competitive inhibitor of the substrate, sucrose. In spite of this result ... | [
-0.08224029839038849,
-0.03822518140077591,
-0.011426905170083046,
0.061675265431404114,
0.035666294395923615,
-0.052529994398355484,
0.05526479333639145,
0.061402320861816406,
-0.04279608279466629,
-0.046722155064344406,
0.03640589118003845,
-0.09181810915470123,
0.014073728583753109,
0.0... |
PMID:19071 | Purification of angiotensin I-converting enzyme from human lung. | Angiotensin I-converting enzyme (peptidyl dipeptide hydrolase, EC 3.4.15.1) was solubilized from the membrane fraction of human lung using trypsin treatment and purfied using columns of DE 52-cellulose, hydroxyapatite and Sephadex G-200. The purified enzyme was shown to convert angiotensin I to angiotensin II and also ... | Purification of angiotensin I-converting enzyme from human lung. Angiotensin I-converting enzyme (peptidyl dipeptide hydrolase, EC 3.4.15.1) was solubilized from the membrane fraction of human lung using trypsin treatment and purfied using columns of DE 52-cellulose, hydroxyapatite and Sephadex G-200. The purified enzy... | [
-0.1182146668434143,
-0.010367249138653278,
-0.060879629105329514,
-0.014346872456371784,
0.0026518087834119797,
-0.0827355608344078,
0.07630413770675659,
0.026565665379166603,
-0.01179597806185484,
-0.02726464346051216,
-0.03468547388911247,
-0.09365689754486084,
-0.005613821092993021,
-0... |
PMID:19072 | Isolation of buffalo muscle aldolase and comparison of its properties with those of rabbit muscle aldolase . | Fructose-1,6-bisphosphate aldolase (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phyosphate-lyase, EC 4.1.2.13) was isolated from buffalo muscle by fractionation with ammonium sulphate and subsequent purification by phosphocellulose column chromatography using a linear salt gradient. As judged by gel filtration and e... | Isolation of buffalo muscle aldolase and comparison of its properties with those of rabbit muscle aldolase . Fructose-1,6-bisphosphate aldolase (D-fructose-1,6-bisphosphate D-glyceraldehyde-3-phyosphate-lyase, EC 4.1.2.13) was isolated from buffalo muscle by fractionation with ammonium sulphate and subsequent purificat... | [
-0.09001390635967255,
-0.0951211228966713,
0.026268988847732544,
0.05389169231057167,
0.0641281008720398,
-0.055261287838220596,
0.03516491502523422,
-0.011587798595428467,
-0.04378300532698631,
0.01126033253967762,
0.03421948105096817,
-0.1019669696688652,
-0.06143123283982277,
-0.0187061... |
PMID:19074 | Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography. | A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently to Sepharose. The method has the advantage of being simple and rapid, thus enabling the purification of th... | Purification of methylmalonyl-CoA mutase from Propionibacterium shermanii using affinity chromatography. A novel procedure for the purification of methylmalonyl-CA mutase from Propionibacterium shermanii has been described which employs affinity chromatography on a column of immobilized vitamin B-12 linked covalently t... | [
-0.09329545497894287,
0.01838250458240509,
-0.08265630900859833,
0.02061464451253414,
0.044798415154218674,
-0.0775827094912529,
0.0031332133803516626,
0.013305950909852982,
-0.040409475564956665,
-0.005166184157133102,
0.028139900416135788,
-0.11563700437545776,
0.05650753900408745,
0.026... |
PMID:19075 | Enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase. | Modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline denaturation as well as tryptic digestion. The specific activity of the modified enzyme is only ... | Enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase. Modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (L-lactate: NAD+ oxidoreductase, EC 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline... | [
-0.06134456768631935,
-0.0022946782410144806,
-0.013309182599186897,
0.062711201608181,
0.02489429898560047,
-0.05697863921523094,
-0.003558267606422305,
0.00045650810352526605,
-0.038572464138269424,
-0.049966949969530106,
0.00233160681091249,
-0.06425420194864273,
0.020106764510273933,
0... |
PMID:19076 | Solubilization and partial characterization of UDP-N-acetylgalactosamine: globoside alpha-N-acetylgalactosaminyltransferase from dog spleen microsomes. | UDP-N-acetylgalactosamine:globoside alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.-) synthesizing Forssman hapten was solubilized from dog spleen microsomes by a combination of Triton X-100 treatment and sonication. The solubilized enzyme was partially purified by calcium phosphate gel, ammonium sulfate fractionati... | Solubilization and partial characterization of UDP-N-acetylgalactosamine: globoside alpha-N-acetylgalactosaminyltransferase from dog spleen microsomes. UDP-N-acetylgalactosamine:globoside alpha-N-acetylgalactosaminyltransferase (EC 2.4.1.-) synthesizing Forssman hapten was solubilized from dog spleen microsomes by a co... | [
-0.07784726470708847,
-0.023416010662913322,
-0.03294766694307327,
-0.05008912831544876,
-0.011846347711980343,
-0.02824520692229271,
0.025896485894918442,
0.022643432021141052,
-0.07005796581506729,
-0.006687144283205271,
0.04539116844534874,
-0.08117510378360748,
0.033229582011699677,
-0... |
PMID:19077 | A conformational change in phosphoglycerate dehydrogenase induced by a shift in pH. | The fluorescence of NADH bound to phosphoglycerate dehydrogenase (3-phosphoglycerate: NAD+ oxidoreductase, EC 1.1.1.95) decreased by 42% between pH 8.5 and 7.0 Serine, an allosteric inhibitor, quenched the fluorescence of enzyme-bound NADH by 29% at pH 8.5, but not at all at pH 7.0. The kinetics of the fluorescence cha... | A conformational change in phosphoglycerate dehydrogenase induced by a shift in pH. The fluorescence of NADH bound to phosphoglycerate dehydrogenase (3-phosphoglycerate: NAD+ oxidoreductase, EC 1.1.1.95) decreased by 42% between pH 8.5 and 7.0 Serine, an allosteric inhibitor, quenched the fluorescence of enzyme-bound N... | [
-0.06571502238512039,
0.034550316631793976,
-0.033520203083753586,
0.06237407401204109,
-0.007905884645879269,
-0.018267448991537094,
0.04155976325273514,
0.0038509799633175135,
-0.0467541478574276,
-0.056095369160175323,
0.03750095143914223,
-0.07396246492862701,
-0.009664272889494896,
0.... |
PMID:19080 | Ganglioside biosynthesis. Characterization of CMP-N-acetylneuraminic acid : lactosylceramide sialyltransferase in Golgi apparatus from rat liver. | An enzyme that transfers sialic acid from GMP-sialic acid to lactosylceramide was concentrated 40-50 times in Golgi apparatus from rat liver relative to total homogenates. This enzyme required detergents as dispersing agents. Of the numerous detergents tested, the combination Tween 80-Triton CF-54 (1 : 2, w/w) was the ... | Ganglioside biosynthesis. Characterization of CMP-N-acetylneuraminic acid : lactosylceramide sialyltransferase in Golgi apparatus from rat liver. An enzyme that transfers sialic acid from GMP-sialic acid to lactosylceramide was concentrated 40-50 times in Golgi apparatus from rat liver relative to total homogenates. Th... | [
0.00046241265954449773,
-0.04135674238204956,
0.012777512893080711,
-0.05265576019883156,
-0.06303153932094574,
-0.05440524220466614,
0.053034063428640366,
0.0620160847902298,
-0.07817444205284119,
-0.01938622258603573,
0.08598869293928146,
-0.09302566945552826,
0.010393532924354076,
0.038... |
PMID:19081 | Thermodynamics of lipid protein associations. Thermodynamics of helix formation in the association of high density apolipoprotein A-I (apoA-I) to dimyristoyl phosphatidylcholine. | The structure and phospholipid-binding properties of human plasma high density apolipoprotein A-I (apoA-I) has been studied at pH 7.4 and 3.1 by microcalorimetry, circular dichroism and density gradient ultracentrifugation. At pH values of 7.4 and 3.1, apoA-I binds to dimyristoyl phosphatidylcholine (DMPC) to form comp... | Thermodynamics of lipid protein associations. Thermodynamics of helix formation in the association of high density apolipoprotein A-I (apoA-I) to dimyristoyl phosphatidylcholine. The structure and phospholipid-binding properties of human plasma high density apolipoprotein A-I (apoA-I) has been studied at pH 7.4 and 3.1... | [
-0.11233571171760559,
0.0007688423502258956,
0.007648675702512264,
-0.008694118820130825,
-0.03468992933630943,
-0.04628774896264076,
0.07058311253786087,
-0.002231317339465022,
-0.015575197525322437,
-0.05184203386306763,
0.017500989139080048,
-0.0867978036403656,
0.0462772436439991,
-0.0... |
PMID:19082 | Characterization and partial purification of acid lipase from human leucocytes. | Hydrolysis of glycerol trioleate by human leucocytes was characterized and the enzymes responsible for this activity were obtained in a purified form by means of gel chromatography on Sephadex G-100 as well as by zonal ultracentrifugation followed by gel chromatography. The activity is localized in the granule fraction... | Characterization and partial purification of acid lipase from human leucocytes. Hydrolysis of glycerol trioleate by human leucocytes was characterized and the enzymes responsible for this activity were obtained in a purified form by means of gel chromatography on Sephadex G-100 as well as by zonal ultracentrifugation f... | [
-0.07332068681716919,
-0.03362360596656799,
-0.052685972303152084,
0.04375072568655014,
0.0009848213521763682,
-0.07903596013784409,
0.04167464002966881,
0.07237204909324646,
-0.01841263473033905,
-0.020153004676103592,
0.0024058890994638205,
-0.08067689836025238,
-0.04262061417102814,
-0.... |
PMID:19083 | The isolation and characterization of sphingomyelinase from human placental tissue. | Human placental sphingomyelinase activity was eluted as a single symmetrical peak from Sephadex G-200 with a molecular weight of 290000; however, the enzyme behaved heterogeneously on ion exchange chromatography. A specific species of sphingomyelinase was purified approx. 10 000-fold to a constant specific activity of ... | The isolation and characterization of sphingomyelinase from human placental tissue. Human placental sphingomyelinase activity was eluted as a single symmetrical peak from Sephadex G-200 with a molecular weight of 290000; however, the enzyme behaved heterogeneously on ion exchange chromatography. A specific species of s... | [
-0.07258586585521698,
-0.04064040631055832,
-0.051530756056308746,
0.004432261921465397,
-0.03185969218611717,
-0.05400655046105385,
0.05056037753820419,
0.07574029266834259,
-0.05670951306819916,
0.03755977749824524,
0.032557010650634766,
-0.06507134437561035,
-0.06125897541642189,
0.0176... |
PMID:19084 | Reaction of selenium with immunoglobulin molecules. | Radioactive selenite reacts with purified human and goat immunoglobulins at acidic and neutral pH. The antigenic properties of the immunoglobulins are retained during the selenium labelling as shown by immunoelectrophoresis and autoradiography. Pepsin digests of 75Se-labelled IgG possess 75Se both in the (Fab')2 fracti... | Reaction of selenium with immunoglobulin molecules. Radioactive selenite reacts with purified human and goat immunoglobulins at acidic and neutral pH. The antigenic properties of the immunoglobulins are retained during the selenium labelling as shown by immunoelectrophoresis and autoradiography. Pepsin digests of 75Se-... | [
-0.020909488201141357,
0.037612948566675186,
-0.020711388438940048,
-0.0008780512143857777,
-0.010473789647221565,
-0.052536461502313614,
0.10188744217157364,
0.03469567000865936,
-0.08585940301418304,
0.03663129732012749,
-0.013372854329645634,
-0.11378618329763412,
-0.013041064143180847,
... |
PMID:19085 | Magnetic circular dichroism studies on acid and alkaline forms of horseradish peroxidase. | The heme vicinities of the acid and alkaline forms of native (Fd(III)) horseradish peroxidase were investigated in terms of the magnetic circular dichroism (MCD) spectroscopy. The MCD spectrum of the acid form of native horseradish peroxidase was characteristic of a ferric high spin heme group. The resemblance in the M... | Magnetic circular dichroism studies on acid and alkaline forms of horseradish peroxidase. The heme vicinities of the acid and alkaline forms of native (Fd(III)) horseradish peroxidase were investigated in terms of the magnetic circular dichroism (MCD) spectroscopy. The MCD spectrum of the acid form of native horseradis... | [
-0.10898624360561371,
-0.002106219297274947,
-0.030951794236898422,
0.05534057319164276,
0.03314770758152008,
-0.05293237417936325,
0.04538485407829285,
0.03532913327217102,
-0.06380884349346161,
-0.031035909429192543,
0.032918404787778854,
-0.006620838772505522,
0.0021426996681839228,
-0.... |
PMID:19086 | Circular dichroism and conformation of human alpha1-antitrypsin. | The solution conformation of alpha 1-antitrypsin from human blood plasma was studied by the circular dichroism (CD) probe. The CD spectra revealed in this glycoprotein approximately 16-20% of alpha-helix, the rest of the main polypeptide chain possessing the pleated sheet (beta) and the aperiodic structures. The confor... | Circular dichroism and conformation of human alpha1-antitrypsin. The solution conformation of alpha 1-antitrypsin from human blood plasma was studied by the circular dichroism (CD) probe. The CD spectra revealed in this glycoprotein approximately 16-20% of alpha-helix, the rest of the main polypeptide chain possessing ... | [
-0.11159282177686691,
-0.01288696750998497,
-0.017381230369210243,
-0.00704333558678627,
0.0031293577048927546,
-0.035879869014024734,
0.05214770510792732,
0.03790964186191559,
-0.03526030480861664,
-0.037108685821294785,
0.0702054351568222,
-0.06559692323207855,
-0.019315136596560478,
0.0... |
PMID:19087 | Iron exchange between transferrin molecules mediated by phosphate compounds and other cell metabolites. | The ability of a large number of cellular metabolites to release iron from transferrin was investigated by measuring the rate at which they could mediate iron exchange between two types of transferrin. Rabbit transferrin labelled with 59Fe was incubated with human apotransferrin in the presence of the metabolites. Afte... | Iron exchange between transferrin molecules mediated by phosphate compounds and other cell metabolites. The ability of a large number of cellular metabolites to release iron from transferrin was investigated by measuring the rate at which they could mediate iron exchange between two types of transferrin. Rabbit transfe... | [
-0.03383966162800789,
-0.017296764999628067,
-0.016595976427197456,
0.03651730343699455,
0.015125241130590439,
-0.07430003583431244,
0.05825003236532211,
0.01519292127341032,
-0.11783011257648468,
-0.061725031584501266,
0.05095742270350456,
-0.08661454170942307,
0.002704295329749584,
-0.00... |
PMID:19088 | Contribution of pH-sensitive metabolic processes to pH homeostasis in isolated rat kidney tubules. | The metabolism of isolated rat kidney tubules suspended in calcium-free physiological saline buffered with phosphate was found to be sensitive to changes in the pH of the suspending medium. Lowering the pH from 7.8 to 6.4 brought about increases in the rates of oxidation of added succinate, glutamate or glutamine as we... | Contribution of pH-sensitive metabolic processes to pH homeostasis in isolated rat kidney tubules. The metabolism of isolated rat kidney tubules suspended in calcium-free physiological saline buffered with phosphate was found to be sensitive to changes in the pH of the suspending medium. Lowering the pH from 7.8 to 6.4... | [
-0.021759195253252983,
-0.0345650240778923,
0.0008106731693260372,
0.031636036932468414,
0.03851115703582764,
-0.05303431674838066,
0.07133480906486511,
0.011365391314029694,
-0.04848862811923027,
0.02315986156463623,
0.047734882682561874,
-0.0910324901342392,
0.05211007967591286,
0.080681... |
PMID:19091 | Purification and properties of rat brain succinic semialdehyde dehydrogenase. | Succinic semialdehyde dehydrogenase from rat brain has been purified to electrophoretic homogeneity. It has a molecular weight of about 140, 000 and is composed of two apparently identical subunits. The reaction catalized by the pure protein is entirely dependent on endogenous --SH groups. The Kim (limits) for NAD and ... | Purification and properties of rat brain succinic semialdehyde dehydrogenase. Succinic semialdehyde dehydrogenase from rat brain has been purified to electrophoretic homogeneity. It has a molecular weight of about 140, 000 and is composed of two apparently identical subunits. The reaction catalized by the pure protein ... | [
-0.05486679822206497,
-0.018031395971775055,
-0.09082816541194916,
-0.039621345698833466,
0.0074188900180161,
0.008558970876038074,
0.050537776201963425,
-0.01008467748761177,
-0.04219341278076172,
0.02357063814997673,
0.0135067543014884,
-0.07272869348526001,
0.03640567883849144,
0.011083... |
PMID:19089 | [Role of phycobilin pigments in photosynthesis]. | Experimental data on possible role of phicobilliprotein (PhBP) in photosynthesis are analysed. It is concluded that the widely spread notion about PhBP only as the light-gathering pigments which transfer the energy to chlorophyll, turned to be insufficiently substantiated experimentally until recently. At the same time... | [Role of phycobilin pigments in photosynthesis]. Experimental data on possible role of phicobilliprotein (PhBP) in photosynthesis are analysed. It is concluded that the widely spread notion about PhBP only as the light-gathering pigments which transfer the energy to chlorophyll, turned to be insufficiently substantiate... | [
-0.07969273626804352,
-0.024846097454428673,
-0.043434567749500275,
0.020354432985186577,
0.0644344836473465,
-0.02854214236140251,
0.07780572026968002,
0.028229068964719772,
-0.07578857243061066,
0.006357318721711636,
0.03031339682638645,
-0.0694495290517807,
-0.012749274261295795,
-0.002... |
PMID:19092 | Presence of a third sucrose hydrolyzing enzyme in Bacillus subtilis: constitutive levanase synthesis by mutants of Bacillus subtilis Marburg 168. | A beta-D-fructofuranosidase -- called levanase -- capable of the hydrolysis of sucrose, inulin and levans has been identified in Bacillus subtilis Marburg. This enzyme can not be detected in strain 168. However, sacL mutations -- mapped on the chromosome of strain 168 between the pheA and aroD reference markers -- lead... | Presence of a third sucrose hydrolyzing enzyme in Bacillus subtilis: constitutive levanase synthesis by mutants of Bacillus subtilis Marburg 168. A beta-D-fructofuranosidase -- called levanase -- capable of the hydrolysis of sucrose, inulin and levans has been identified in Bacillus subtilis Marburg. This enzyme can no... | [
-0.05062813684344292,
-0.022927135229110718,
-0.08517787605524063,
0.010304451920092106,
0.030541229993104935,
-0.01963016577064991,
-0.013010067865252495,
0.02407425455749035,
-0.023616380989551544,
0.03125930577516556,
-0.006422823760658503,
-0.15153329074382782,
-0.02354186400771141,
0.... |
PMID:19093 | [Genetic independence of two forms of carbonic anhydrase from bovine erythrocytes]. | The two major forms of bovine erythrocyte carbonic anhydrase have been designated as CI and CII because their high activity of the C type. Separation of both forms and isolation of CI from the ethanol chloroform extract of the hemolysate were obtained by either column chromatography on DEAE-cellulose DE 23 or on DEAE-s... | [Genetic independence of two forms of carbonic anhydrase from bovine erythrocytes]. The two major forms of bovine erythrocyte carbonic anhydrase have been designated as CI and CII because their high activity of the C type. Separation of both forms and isolation of CI from the ethanol chloroform extract of the hemolysat... | [
-0.09242761880159378,
-0.019779659807682037,
-0.040159184485673904,
0.07735250890254974,
0.004310022573918104,
-0.028412126004695892,
0.0776609554886818,
0.03504003956913948,
-0.028059961274266243,
-0.0027250279672443867,
-0.027066294103860855,
-0.0915946215391159,
-0.06366045773029327,
0.... |
PMID:19095 | A fine-structural study of embryonic and larval development in the gymnoblastic hydroid Pennaria tiarella. | 1. The pregastrulation blastomers contain electron-dense granules which become localized after gastrulation in the apices of the developing epithelio-muscle cells and persist throughout larval development. The cytoplasm of the blastomeres is organized into anucleate, membrane-delimited lobules. The lobules, which persi... | A fine-structural study of embryonic and larval development in the gymnoblastic hydroid Pennaria tiarella. 1. The pregastrulation blastomers contain electron-dense granules which become localized after gastrulation in the apices of the developing epithelio-muscle cells and persist throughout larval development. The cyt... | [
-0.008103621192276478,
-0.10099223256111145,
0.02200532704591751,
0.00822888221591711,
-0.017437173053622246,
0.0010385534260421991,
-0.0054741245694458485,
0.030000686645507812,
-0.02181178517639637,
0.0004671331262215972,
0.022741204127669334,
-0.0748942643404007,
-0.005049987696111202,
... |
PMID:19096 | [Identification and properties of 2 forms of plant phospholipase D]. | Effect of different factors (pH, temperature etc.) on the activity of phospholipase D is studied. It is found that plant phospholipase D has two forms: thermolabile (D1) and thermostable (Ds). Both forms are shown to differ in some characteristics (pH optimum, temperature optimum, calcium ions activation, activator eff... | [Identification and properties of 2 forms of plant phospholipase D]. Effect of different factors (pH, temperature etc.) on the activity of phospholipase D is studied. It is found that plant phospholipase D has two forms: thermolabile (D1) and thermostable (Ds). Both forms are shown to differ in some characteristics (pH... | [
-0.07112052291631699,
-0.02029457315802574,
0.08682242780923843,
-0.012017671018838882,
0.000602487416472286,
-0.02024061791598797,
0.08767931908369064,
0.056020766496658325,
-0.06369072198867798,
-0.036465223878622055,
0.011328306049108505,
-0.07971170544624329,
-0.006116658914834261,
-0.... |
PMID:19097 | [Isolation and properties of leucine aminopeptidase from Aspergillus oryzae]. | Homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of Asperigillus oryzae using treatment with activated characoal, followed by DEAE-cellulose and hydroxylapatite chromatographies, Biogel P-100 gel-filtration and polyacrylamide-gel electrophoresis. The enzyme h... | [Isolation and properties of leucine aminopeptidase from Aspergillus oryzae]. Homogenious leucine aminopeptidase is purified from "oryzine"--mixture of enzymes produced by surface culture of Asperigillus oryzae using treatment with activated characoal, followed by DEAE-cellulose and hydroxylapatite chromatographies, Bi... | [
-0.08708019554615021,
0.0038621127605438232,
-0.076650470495224,
0.01869056187570095,
-0.051947418600320816,
-0.049604084342718124,
0.08145478367805481,
0.07732995599508286,
-0.052189622074365616,
-0.0014040687819942832,
0.033674001693725586,
-0.036310214549303055,
0.014021795243024826,
0.... |
PMID:19098 | [Malic acid induction of decarboxylating NADP-malate dehydrogenase synthesis in C3-plant leaves]. | The activity of decarboxylating NADP-malatedehydrogenase (E. C. 1.1.1.40) in green ethiolated pea and barley leaves and in green leaves of a pea mutant lacking photosystem II is found to be 3-fold increased after the injection of malic acid into cut plants. Protein synthesis inhibitors depressed malic acid-induced incr... | [Malic acid induction of decarboxylating NADP-malate dehydrogenase synthesis in C3-plant leaves]. The activity of decarboxylating NADP-malatedehydrogenase (E. C. 1.1.1.40) in green ethiolated pea and barley leaves and in green leaves of a pea mutant lacking photosystem II is found to be 3-fold increased after the injec... | [
-0.02277575060725212,
-0.025371616706252098,
0.04352537542581558,
0.023180071264505386,
0.08439166843891144,
0.00011524548608576879,
0.02714020386338234,
0.033888790756464005,
-0.0764094814658165,
0.013361857272684574,
0.04768548160791397,
-0.0904889777302742,
0.06911849230527878,
0.006019... |
PMID:19099 | [Characteristics of acid ribonuclease from rat thymus chromatin]. | Acid ribonuclease, free of nucleases and phosphatases, is isolated from rat thymus chromatin. The pH optimum of the enzyme is 5.0-5.5, optimal concentrations of Na+ and K+ ions are 0.05-0.15 M and 0.05 M respectively, Mg2+ inhibits the enzyme activity. The enzyme hydrolyses poly U, poly AU, cytoplasmic and nuclear RNAs... | [Characteristics of acid ribonuclease from rat thymus chromatin]. Acid ribonuclease, free of nucleases and phosphatases, is isolated from rat thymus chromatin. The pH optimum of the enzyme is 5.0-5.5, optimal concentrations of Na+ and K+ ions are 0.05-0.15 M and 0.05 M respectively, Mg2+ inhibits the enzyme activity. T... | [
-0.05222814902663231,
-0.009327517822384834,
-0.03716375306248665,
0.012360462918877602,
-0.017437981441617012,
-0.021978825330734253,
0.03329194709658623,
0.042748838663101196,
-0.039163634181022644,
0.01655549369752407,
0.0553620420396328,
-0.06474708020687103,
-0.03367578983306885,
-0.0... |
PMID:19100 | [Purification of phospholipase C from Bacillus cereus by chromatography on aminoalkylpolysaccharide adsorbents]. | Purification of phospholipase C from Bac. cereus by chromatography on aminoalkylpolysaccharide adsorbents is described. The dependence of the degree of enzyme purification on the amount of ligant and effect of pH and buffer systems on the adsorption-desorption of phospholipase have been studied. At a pH below 9.0 phosp... | [Purification of phospholipase C from Bacillus cereus by chromatography on aminoalkylpolysaccharide adsorbents]. Purification of phospholipase C from Bac. cereus by chromatography on aminoalkylpolysaccharide adsorbents is described. The dependence of the degree of enzyme purification on the amount of ligant and effect ... | [
-0.03910554572939873,
-0.0019050684059038758,
-0.02009410597383976,
-0.024329623207449913,
-0.04241633415222168,
-0.08957896381616592,
0.05261412635445595,
0.1035301461815834,
-0.03052467107772827,
0.006573679391294718,
0.01208652276545763,
-0.07732358574867249,
-0.004792379681020975,
0.00... |
PMID:19101 | [pH-dependence characteristics of Ca-ATPase activity of heavy meromyosin with modified SH-groups]. | Study of pH-dependence of Ca-ATPase activity of heavy meromyosin (HMM) at low and high ionic strength showed essential differences in the modifying effect of two sulfhydryl reagents, p-CMB and silver. Silver ions in conditions studied independently on pH and KCl concentration produce an inhibition of ATP hydrolysis by ... | [pH-dependence characteristics of Ca-ATPase activity of heavy meromyosin with modified SH-groups]. Study of pH-dependence of Ca-ATPase activity of heavy meromyosin (HMM) at low and high ionic strength showed essential differences in the modifying effect of two sulfhydryl reagents, p-CMB and silver. Silver ions in condi... | [
-0.08587764948606491,
0.020927023142576218,
-0.016088254749774933,
0.019567696377635002,
-0.001240622135810554,
-0.007655899506062269,
0.03200899809598923,
0.008212417364120483,
-0.02822364680469036,
-0.019894104450941086,
0.02200046367943287,
-0.038426920771598816,
-0.025200964882969856,
... |
PMID:19102 | [Regulation of glutamine metabolism in Chlorella pyrenoidosa. Regulation of glutamine synthetase activity by adenylic system components]. | A decrease of glutamine synthetase (E. C. 6.3.1.2.) activity was observed under the assimilation of ammonium nitrogen in Chlorella. At the same time a decrease of ATP content in Chlorella cells took place. The ATP content was 7-fold decreased, while ADP and AMP contents were 4-fold and 3-fold increased respectively, af... | [Regulation of glutamine metabolism in Chlorella pyrenoidosa. Regulation of glutamine synthetase activity by adenylic system components]. A decrease of glutamine synthetase (E. C. 6.3.1.2.) activity was observed under the assimilation of ammonium nitrogen in Chlorella. At the same time a decrease of ATP content in Chlo... | [
-0.026745174080133438,
-0.06185881420969963,
-0.02600838430225849,
0.000413080066209659,
0.0436447337269783,
0.00866636261343956,
0.054122794419527054,
0.019404977560043335,
-0.08193953335285187,
-0.003484719432890415,
0.04502445086836815,
-0.09155339747667313,
0.03891007974743843,
0.02249... |
PMID:19103 | [Oxidation of putrescine, spermidine and spermine by diamino-oxidase from mouse liver]. | Optimal conditions to determine the activity of diaminooxidase in mouse liver homogenate are described. Maximal oxidation rate for putrescine was found to take place at a concentration of 20 mM and pH 9.5, and for spermidine and spermine--at 10 mM concentration and pH 9.2. The rate of tyramine oxidation was maximal at ... | [Oxidation of putrescine, spermidine and spermine by diamino-oxidase from mouse liver]. Optimal conditions to determine the activity of diaminooxidase in mouse liver homogenate are described. Maximal oxidation rate for putrescine was found to take place at a concentration of 20 mM and pH 9.5, and for spermidine and spe... | [
-0.06387162208557129,
0.025954537093639374,
-0.007789999712258577,
-0.019087569788098335,
-0.010713947005569935,
0.01465911790728569,
0.030704475939273834,
0.040150269865989685,
-0.07659055292606354,
-0.007744616828858852,
0.03434046357870102,
-0.05140191689133644,
-0.023180164396762848,
0... |
PMID:19104 | [Fluorescent properties of b-type ferredoxins]. | Fluroescent spectra of six b-type ferredoxins of plant and animal origins were obtained. All investigated proteins do not contain tryptophan. The emission maxima of the native proteins, apoproteins prepared by various methods, and denaturated proteins are compared. The effects of pH, ionic strength and ferricyanide on ... | [Fluorescent properties of b-type ferredoxins]. Fluroescent spectra of six b-type ferredoxins of plant and animal origins were obtained. All investigated proteins do not contain tryptophan. The emission maxima of the native proteins, apoproteins prepared by various methods, and denaturated proteins are compared. The ef... | [
-0.05201941356062889,
-0.012013111263513565,
-0.02703149989247322,
0.03718994930386543,
0.026504667475819588,
-0.00431377487257123,
0.04663042724132538,
0.04040246456861496,
-0.0302033182233572,
-0.03548755124211311,
-0.01132539939135313,
-0.0984354168176651,
-0.003138116095215082,
0.05044... |
PMID:19105 | [Relationships of photoreduction of substituted salts of 4,4'-dipyridyl by isolated chloroplasts]. | Kinetic relationships of photoreduction of 4,4'-dimethyldipyridyl and 4,4'-dibenzyldipyridyl in the presence of chlorplast were studied. It was found that photoreduction leads to the establishment of photosteady state in which the rate of photoreduction is equal to the rate of dark oxidation of reduced form. Kinetics o... | [Relationships of photoreduction of substituted salts of 4,4'-dipyridyl by isolated chloroplasts]. Kinetic relationships of photoreduction of 4,4'-dimethyldipyridyl and 4,4'-dibenzyldipyridyl in the presence of chlorplast were studied. It was found that photoreduction leads to the establishment of photosteady state in ... | [
-0.05240117758512497,
0.006743546109646559,
-0.03656414523720741,
0.05492224171757698,
0.0457540862262249,
-0.008527490310370922,
0.02123267948627472,
0.018043993040919304,
-0.09816090762615204,
-0.04690202325582504,
0.004044846165925264,
-0.02470122091472149,
0.04066271707415581,
0.014279... |
PMID:19106 | [Some pathways of biosynthesis and degradation of polyphosphates from green algae Acetabularia mediterranea]. | The activity of ATP: polyphosphate phosphotransferase was detected in free-cellular extracts of Acetabularia mediterranea. The enzyme activity in cells originally deficient in phosphorus and subsequently transferred into the phosphate-containing medium increases 5-10-fold as compared to normal. Polyphosphate degradatio... | [Some pathways of biosynthesis and degradation of polyphosphates from green algae Acetabularia mediterranea]. The activity of ATP: polyphosphate phosphotransferase was detected in free-cellular extracts of Acetabularia mediterranea. The enzyme activity in cells originally deficient in phosphorus and subsequently transf... | [
-0.03924168646335602,
0.010035866871476173,
0.042638517916202545,
-0.014581028372049332,
0.010179025121033192,
-0.05572737380862236,
0.03126437962055206,
0.03888292983174324,
-0.03684350848197937,
-0.015458274632692337,
0.05228614807128906,
-0.0975300595164299,
-0.014914564788341522,
-0.02... |
PMID:19107 | [Photophosphorylation, sensitized by chlorophills a and b, pheophytin and beta-carotene in a modelled system]. | In aqueous solutions in air atmosphere chlorophylls a and b, pheophytin and beta-carotene adsorbed on aluminium oxide powder are capable of sensibilizing electron transfer from phosphate ions coupled with the formation of high energy bonds of adenosine phosphates. The highest activity of chlorophylls a and b and pheoph... | [Photophosphorylation, sensitized by chlorophills a and b, pheophytin and beta-carotene in a modelled system]. In aqueous solutions in air atmosphere chlorophylls a and b, pheophytin and beta-carotene adsorbed on aluminium oxide powder are capable of sensibilizing electron transfer from phosphate ions coupled with the ... | [
-0.0648806244134903,
0.006462712772190571,
-0.004054146818816662,
0.028109122067689896,
0.026876896619796753,
-0.018844079226255417,
0.05319835618138313,
-0.008588865399360657,
-0.08297376334667206,
-0.007630275096744299,
0.037520311772823334,
-0.043694399297237396,
0.00488304765895009,
0.... |
PMID:19109 | Structure and fragmentation mechanisms of some ions in the mass spectrum of ephedrine. | This paper describes the use of gas chromatography mass spectrometry for the confirmation of ephedrine in adulterated powdered ipecac and ipecac fluid extract. Deuteration studies of ephedrine hydrochloride and some related compounds were also performed in an attempt to postulate structures for some of the fragment ion... | Structure and fragmentation mechanisms of some ions in the mass spectrum of ephedrine. This paper describes the use of gas chromatography mass spectrometry for the confirmation of ephedrine in adulterated powdered ipecac and ipecac fluid extract. Deuteration studies of ephedrine hydrochloride and some related compounds... | [
-0.07284320145845413,
-0.015732038766145706,
0.03154546394944191,
-0.03456534445285797,
0.02711428515613079,
0.021739406511187553,
0.029701106250286102,
0.035476360470056534,
-0.04239471256732941,
-0.01354251429438591,
0.006585526745766401,
-0.055587779730558395,
-0.041961342096328735,
-0.... |
PMID:19110 | Process engineering studies on immobilized trypsin using molecular sieves as carriers. | In the present investigation molecular sieve has been found to be a suitable carrier for the immobilization of enzymes. From the present study it may be specifically found that trypsin and pappain can be immobilized by molecular sieve type 4a following a very simple techniqure. The immobilized enzyme can be used both i... | Process engineering studies on immobilized trypsin using molecular sieves as carriers. In the present investigation molecular sieve has been found to be a suitable carrier for the immobilization of enzymes. From the present study it may be specifically found that trypsin and pappain can be immobilized by molecular siev... | [
-0.07348356395959854,
0.01633397489786148,
-0.008176105096936226,
-0.0185602605342865,
-0.037842221558094025,
-0.0465364083647728,
0.03037722036242485,
0.03170549497008324,
-0.08286043256521225,
-0.03939460590481758,
0.021261345595121384,
-0.01301211304962635,
-0.05252429097890854,
0.01338... |
PMID:19111 | Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus. | An extracellular polymer was produced by continuous fermentation of Corynebacterium hydrocarboclastus on kerosene in a 24 liter reactor. This polymer was composed of protein, lipid, and carbohydrates. The polymer possessed surface active properties, and had two critical micelle concentrations. Its effectiveness was qui... | Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus. An extracellular polymer was produced by continuous fermentation of Corynebacterium hydrocarboclastus on kerosene in a 24 liter reactor. This polymer was composed of protein, lipid, and carbohydrates. The polymer possessed surface ... | [
-0.04986371099948883,
0.02356833405792713,
0.0038560538087040186,
0.01859954185783863,
0.026628417894244194,
-0.024015504866838455,
0.01054371241480112,
0.06609220057725906,
-0.05059678480029106,
0.018552230671048164,
-0.003687078831717372,
-0.09620413184165955,
-0.035899627953767776,
-0.0... |
PMID:19112 | 1,4-alpha-Glucan phosphorylase form Klebsiella pneumoniae covalently couple on porous glass. | A simplified procedure for the preparation of 1,4-alpha-glucan phosphorylase from Klebsiella pneumoniae is described. An 80-fold purification is achieved in two steps with an overall yield of about 50%. The specific activity of the homogeneous enzyme protein is 17.7 units/mg. Compared with glycogen phosphorylase from r... | 1,4-alpha-Glucan phosphorylase form Klebsiella pneumoniae covalently couple on porous glass. A simplified procedure for the preparation of 1,4-alpha-glucan phosphorylase from Klebsiella pneumoniae is described. An 80-fold purification is achieved in two steps with an overall yield of about 50%. The specific activity of... | [
-0.06309235841035843,
-0.02519594505429268,
-0.0053893146105110645,
-0.02807287499308586,
0.04005247354507446,
-0.09300407767295837,
0.008615429513156414,
0.003968773875385523,
-0.047467585653066635,
-0.02104724384844303,
-0.01675347611308098,
-0.07074669748544693,
-0.060721505433321,
0.02... |
PMID:19114 | Prophylactiv use of propranolol in the Marfan syndrome to prevent aortic dissection. | Twenty-five patients, 16 men and 9 women, 5-59 years of age with the Marfan syndrome and cardiac complications were started on propranolol over a 6-year period. Propranolol was used as a beta-adrenergic blocking agent to reduce myocardial contractility in an attempt thereby to stay the progression of aortic dilatation ... | Prophylactiv use of propranolol in the Marfan syndrome to prevent aortic dissection. Twenty-five patients, 16 men and 9 women, 5-59 years of age with the Marfan syndrome and cardiac complications were started on propranolol over a 6-year period. Propranolol was used as a beta-adrenergic blocking agent to reduce myocard... | [
-0.03911083564162254,
0.016377493739128113,
0.021154405549168587,
0.017070557922124863,
-0.041886504739522934,
-0.016508106142282486,
-0.027581145986914635,
0.05805554240942001,
-0.016386499628424644,
-0.0646854117512703,
0.019176751375198364,
0.0011394531466066837,
0.0005322586512193084,
... |
PMID:19115 | The interaction of some bis-arylhydroxysulphonic acids with a site of known structure in human haemoglobin. | 1 Two bis-arylhydroxysulphonic acids were previously designed to interact with the known molecular configuration of the 2,3-diphosphoglycerate (DPG) receptor-site of human haemoglobin. These compounds liberate oxygen from the haemoglobin similarly to DPG. 2 Solutions of haemoglobin have now been observed under physiolo... | The interaction of some bis-arylhydroxysulphonic acids with a site of known structure in human haemoglobin. 1 Two bis-arylhydroxysulphonic acids were previously designed to interact with the known molecular configuration of the 2,3-diphosphoglycerate (DPG) receptor-site of human haemoglobin. These compounds liberate ox... | [
-0.10377656668424606,
0.04981709644198418,
-0.05093058943748474,
-0.01909921132028103,
-0.004580679349601269,
-0.03785542771220207,
0.033939655870199203,
0.01998748816549778,
-0.03179919719696045,
-0.04442095384001732,
0.04990505427122116,
-0.07331902533769608,
0.017296526581048965,
-0.001... |
PMID:19116 | A potent new beta2-adrenoceptor blocking agent. | 1 (t-Butyl-amino-3-ol-2-propyl) oximino-9 fluorene is a new beta2-adrenoceptor blocking agent with a pA2 of 9.23+/-0.25 on isolated trachea. 2 It provokes hypertension in normotensive rats and does not prevent arterial hypertension in SHR rats, although it does prevent the renin secretion normally induced by isoprenali... | A potent new beta2-adrenoceptor blocking agent. 1 (t-Butyl-amino-3-ol-2-propyl) oximino-9 fluorene is a new beta2-adrenoceptor blocking agent with a pA2 of 9.23+/-0.25 on isolated trachea. 2 It provokes hypertension in normotensive rats and does not prevent arterial hypertension in SHR rats, although it does prevent th... | [
-0.02943427674472332,
-0.030983390286564827,
-0.038188010454177856,
0.026111356914043427,
-0.02060232125222683,
-0.03045203723013401,
0.03656371682882309,
-0.01633821800351143,
-0.0078122783452272415,
-0.10040932148694992,
-0.03582891821861267,
-0.035203684121370316,
0.009828290902078152,
... |
PMID:19117 | Some observations on the mechanism of benzodiazepine-barbiturate interactions in the mouse. | 1 The prolongation of pentobarbitone sleeping by five benzodiazepines, administered by prior intraperitoneal injection, was measured in mice. The pentobarbitone was injected either intraperitoneally or intracerebroventricularly. For each benzodiazepine, the prolongation was dose-related and differences in potency betwe... | Some observations on the mechanism of benzodiazepine-barbiturate interactions in the mouse. 1 The prolongation of pentobarbitone sleeping by five benzodiazepines, administered by prior intraperitoneal injection, was measured in mice. The pentobarbitone was injected either intraperitoneally or intracerebroventricularly.... | [
-0.0804300382733345,
0.02496233768761158,
0.01177426241338253,
0.012770353816449642,
0.02968955598771572,
0.007438391447067261,
0.059270456433296204,
0.011850889772176743,
-0.03919854015111923,
-0.0333976075053215,
-0.02215389534831047,
-0.01517392136156559,
-0.005116581451147795,
0.016832... |
PMID:19118 | Studies on the cardiovascular effects of pindolol in DOCA/saline hypertensive rats. | 1 A hypotensive response to orally administered pindolol in conscious normotensive and deoxycorticosterone acetate (DOCA)/saline hypertensive rats (DS-rats) is described. In DS-rats, pindolol (10-50 mug/kg) produced a dose-dependent fall in blood pressure and elevation of resting heart rate.2 The hypotensive response a... | Studies on the cardiovascular effects of pindolol in DOCA/saline hypertensive rats. 1 A hypotensive response to orally administered pindolol in conscious normotensive and deoxycorticosterone acetate (DOCA)/saline hypertensive rats (DS-rats) is described. In DS-rats, pindolol (10-50 mug/kg) produced a dose-dependent fal... | [
-0.030973514541983604,
0.05172835662961006,
0.037180110812187195,
-0.0021083985920995474,
0.016308270394802094,
-0.022089051082730293,
0.02567385695874691,
0.0073808711022138596,
-0.04090854525566101,
-0.04222077131271362,
0.0037359921261668205,
-0.02708050049841404,
-0.008364429697394371,
... |
PMID:19119 | An evaluation of viability tests of human cadaveric kidneys. | Cadaveric kidneys are sometimes unsuitable for transplantation because of possible ischaemic damage. The advent of perfusion preservation machines has enabled evaluation of perfusion characteristics and perfusate changes of organs prior to transplantation. This study has evaluated the changes in perfusate pH, lacate de... | An evaluation of viability tests of human cadaveric kidneys. Cadaveric kidneys are sometimes unsuitable for transplantation because of possible ischaemic damage. The advent of perfusion preservation machines has enabled evaluation of perfusion characteristics and perfusate changes of organs prior to transplantation. Th... | [
-0.0667591318488121,
0.013937595300376415,
0.05477277189493179,
0.07191268354654312,
-0.022265378385782242,
-0.04827483370900154,
0.09118928015232086,
0.061879344284534454,
-0.035730648785829544,
0.011022434569895267,
-0.01442804466933012,
-0.055592697113752365,
0.0013431746046990156,
-0.0... |
PMID:19120 | Where is your vagotomy incomplete? Observations on operative technique. | There is a trend in gastric surgery towards more selective types of vagotomy but the techniques are more difficult and incomplete nerve section may be more likely. Using the Grassi intra-operative test of mucosal pH, we have studied 50 consecutive patients, 13 having truncal vagotomy, 9 having bilateral selective vagot... | Where is your vagotomy incomplete? Observations on operative technique. There is a trend in gastric surgery towards more selective types of vagotomy but the techniques are more difficult and incomplete nerve section may be more likely. Using the Grassi intra-operative test of mucosal pH, we have studied 50 consecutive ... | [
0.06109628081321716,
0.006007902789860964,
0.02677868865430355,
0.022509660571813583,
-0.03255915641784668,
0.029966434463858604,
0.03440823033452034,
0.023791691288352013,
0.0223382581025362,
-0.0024034471716731787,
-0.04669371247291565,
-0.06994656473398209,
-0.05527174100279808,
0.06110... |
PMID:19122 | Increased exercise tolerance with nitrates in beta-blockaded patients with angina. | In 14 beta-blockaded anginal subjects, 10 of whom had poor left ventricular function, sublingual isosorbide dinitrate significantly increased maximal exercise capacity on a standardized multistage treadmill test. This was associated with changes in heart rate and blood pressure suggestive of a fall in left ventricular ... | Increased exercise tolerance with nitrates in beta-blockaded patients with angina. In 14 beta-blockaded anginal subjects, 10 of whom had poor left ventricular function, sublingual isosorbide dinitrate significantly increased maximal exercise capacity on a standardized multistage treadmill test. This was associated with... | [
-0.042271848767995834,
0.0027592002879828215,
0.011324943974614143,
0.04879487678408623,
0.016892746090888977,
0.04849892854690552,
0.0591779462993145,
0.007818058133125305,
-0.0305777620524168,
-0.02192007564008236,
-0.0006579879554919899,
-0.06772078573703766,
0.018920987844467163,
0.062... |
PMID:19125 | A serotonergic innervation of noradrenergic neurons in nucleus locus coeruleus: demonstration by immunocytochemical localization of the transmitter specific enzymes tyrosine and tryptophan hydroxylase. | Immunocytochemical localization of the neurotransmitter synthesizing enzymes, tyrosine and tryptophan hydroxylase, was used to determine whether the noradrenergic neurons in the nucleus locus coeruleus of the rat are innervated by serotonergic (5-HT) neurons. Specific antibodies were prepared to tyrosine hydroxylase, p... | A serotonergic innervation of noradrenergic neurons in nucleus locus coeruleus: demonstration by immunocytochemical localization of the transmitter specific enzymes tyrosine and tryptophan hydroxylase. Immunocytochemical localization of the neurotransmitter synthesizing enzymes, tyrosine and tryptophan hydroxylase, was... | [
0.010540367104113102,
-0.04043271392583847,
-0.058632493019104004,
-0.007388224825263023,
-0.01890951208770275,
0.0008139681303873658,
0.07676685601472855,
0.003716615727171302,
0.014241375029087067,
-0.05775848776102066,
0.03960186988115311,
-0.07092370092868805,
-0.004771803971379995,
0.... |
PMID:19128 | Behavioral effects of LSD in the cat: proposal of an animal behavior model for studying the actions of hallucinogenic drugs. | In the course of examining the complete dose-response relationship for the behavioral effects of LSD in the cat, we discovered that, in addition to large increases in investigatory and hallucinatory-like responses, two behaviors, not previously reported, are emitted with a high probability under LSD. Beginning from a b... | Behavioral effects of LSD in the cat: proposal of an animal behavior model for studying the actions of hallucinogenic drugs. In the course of examining the complete dose-response relationship for the behavioral effects of LSD in the cat, we discovered that, in addition to large increases in investigatory and hallucinat... | [
-0.00587640842422843,
-0.0014580253046005964,
0.05026768520474434,
0.032204098999500275,
0.02149438112974167,
0.03910643234848976,
0.10628408193588257,
0.0475090928375721,
0.0014662714675068855,
-0.01557452417910099,
0.033013712614774704,
0.0015554032288491726,
-0.01804882287979126,
0.0430... |
PMID:19132 | [Influence of age and sex on the acute toxicity of two steroid anesthetics in rats]. | During the newborn period, there is a hight increase of the acute toxicity of two steroid anaesthetics (Hydroxydione, Althesin) given by intraperitoneal route on the rat as also an influence of hormonal conditions. | [Influence of age and sex on the acute toxicity of two steroid anesthetics in rats]. During the newborn period, there is a hight increase of the acute toxicity of two steroid anaesthetics (Hydroxydione, Althesin) given by intraperitoneal route on the rat as also an influence of hormonal conditions. | [
-0.017661869525909424,
-0.026042597368359566,
-0.00003914552507922053,
-0.03611711412668228,
-0.03620338812470436,
0.024487720802426338,
0.05655283480882645,
0.010456266812980175,
-0.05562220513820648,
-0.043385472148656845,
-0.04029544070363045,
0.0017886292189359665,
-0.0007609514868818223... |
PMID:19133 | [Lysine decarboxylase in Pseudomonas aeruginosa]. | The research of lysine, ornithine and arginine decarboxylases has been made for 50 strains of fluorescent Pseudomonas (P. aeruginosa, P. fluorescens, P. putida). By thin layer chromatography, all the strains of Pseudomonas aeruginosa and the fifth of the strains of P. putida had lysine decarboxylase activity at alcalin... | [Lysine decarboxylase in Pseudomonas aeruginosa]. The research of lysine, ornithine and arginine decarboxylases has been made for 50 strains of fluorescent Pseudomonas (P. aeruginosa, P. fluorescens, P. putida). By thin layer chromatography, all the strains of Pseudomonas aeruginosa and the fifth of the strains of P. p... | [
-0.03929196298122406,
-0.03828198462724686,
-0.07834520936012268,
0.04771451652050018,
-0.01233562733978033,
-0.005091926548629999,
-0.0224210973829031,
0.03467806056141853,
-0.0376061275601387,
-0.0202979426831007,
-0.004217523615807295,
-0.074335016310215,
0.03442596644163132,
0.03163322... |
PMID:19134 | Cathepsin D activity in isolated odontoblasts. | The presence of an acid proteinase with a high activity has been demonstrated in isolated odontoblast-predentine material from dentinogenically active rat incisors. The enzyme was identified as cathepsin D (EC 3.4.23.5). The possible significance of the enzymatic degradation of proteoglycans and glycosaminoglycans in t... | Cathepsin D activity in isolated odontoblasts. The presence of an acid proteinase with a high activity has been demonstrated in isolated odontoblast-predentine material from dentinogenically active rat incisors. The enzyme was identified as cathepsin D (EC 3.4.23.5). The possible significance of the enzymatic degradati... | [
-0.06689441949129105,
-0.019135352224111557,
-0.006858539767563343,
-0.010847726836800575,
-0.056919123977422714,
-0.04172610864043236,
0.03855421021580696,
0.04483210667967796,
-0.018204685300588608,
0.016751185059547424,
0.028300393372774124,
-0.10899007320404053,
-0.013918103650212288,
... |
PMID:19135 | An in vitro and in vivo investigation of mellitate and ethane-1-hydroxy-1,1-diphosphonate in calcium phosphate systems. | Comparisons of mellitate (MA) and ethane-1-hydroxy-1,1-diphosphonate (EHDP) have been carried out in studies of enamel etching, calcium phosphate crystal growth and animal calculus deposition. In enamel etch studies at pH 5, 6, or 7 and after treatment times of 5 or 170 min, EHDP was less damaging to enamel surfaces th... | An in vitro and in vivo investigation of mellitate and ethane-1-hydroxy-1,1-diphosphonate in calcium phosphate systems. Comparisons of mellitate (MA) and ethane-1-hydroxy-1,1-diphosphonate (EHDP) have been carried out in studies of enamel etching, calcium phosphate crystal growth and animal calculus deposition. In enam... | [
-0.06346286833286285,
-0.023240555077791214,
0.09787198901176453,
0.023388026282191277,
-0.035951610654592514,
-0.04418383538722992,
0.0033909848425537348,
0.08093797415494919,
-0.08659588545560837,
0.013399974443018436,
0.027703261002898216,
-0.0466579906642437,
0.03462003916501999,
0.016... |
PMID:19136 | Pentose-shunt oxidation in the periosteal cells in healing fractures. | The activity of pentose-shunt dehydrogenases is very low in periosteal cells of normal rat metatarsals, but increases one day post-fracture and rises linearly over the next two days. By four days post-fracture, the distribution of this activity along the bone shows two centres of high activity: the first in the region ... | Pentose-shunt oxidation in the periosteal cells in healing fractures. The activity of pentose-shunt dehydrogenases is very low in periosteal cells of normal rat metatarsals, but increases one day post-fracture and rises linearly over the next two days. By four days post-fracture, the distribution of this activity along... | [
-0.05079681798815727,
-0.02456100471317768,
-0.021635305136442184,
-0.006039614323526621,
0.03278515115380287,
-0.015228992328047752,
0.04382066801190376,
0.03307157754898071,
-0.019409114494919777,
-0.027178654447197914,
-0.005781220272183418,
-0.0503322072327137,
0.04097675159573555,
0.0... |
PMID:19137 | The inhibition of calcium oxalate crystal growth by multidentate organic phosphonates. | The effect of a number of structurally related multidentate organic phosphonates on the rate of crystal growth of calcium oxalate was studied as a function of pH. Rate constants were obtained at various concentrations for the phosphonates ethane-1-hydroxy-1,1-diphosphonate (EHDP), nitrilotri(methylenephosphonic acid) (... | The inhibition of calcium oxalate crystal growth by multidentate organic phosphonates. The effect of a number of structurally related multidentate organic phosphonates on the rate of crystal growth of calcium oxalate was studied as a function of pH. Rate constants were obtained at various concentrations for the phospho... | [
-0.05949827656149864,
-0.0315275713801384,
0.01410477701574564,
-0.014442641288042068,
-0.057734765112400055,
-0.037114012986421585,
0.009600997902452946,
0.027601569890975952,
-0.0757373720407486,
-0.007760906126350164,
0.04303392767906189,
-0.060289349406957626,
0.06716107577085495,
0.04... |
PMID:19138 | Comparative toxicities of enflurane, fluroxene and nitrous oxide at subanaesthetic concentrations in laboratory animals. | We compared the toxicities of subanesthetic concentrations of fluroxene, enflurane and nitrous oxide in mice, rats and guinea pigs which were in an active growth phase. Fluroxene produced a greater mortality and decrement in weight gain than enflurane and nitrous oxide despite administration of far lower concentrations... | Comparative toxicities of enflurane, fluroxene and nitrous oxide at subanaesthetic concentrations in laboratory animals. We compared the toxicities of subanesthetic concentrations of fluroxene, enflurane and nitrous oxide in mice, rats and guinea pigs which were in an active growth phase. Fluroxene produced a greater m... | [
0.023914121091365814,
-0.008201070129871368,
0.040842730551958084,
-0.015677042305469513,
0.02468349225819111,
0.011709616519510746,
0.03255371376872063,
0.05658145993947983,
-0.0011722248746082187,
-0.010301345959305763,
-0.05403315648436546,
-0.05964692309498787,
0.019818240776658058,
0.... |
PMID:19139 | Identification of beta adrenergic receptors in rat hypothalamus. | (-)-[3H]-Dihydroalprenolol((-)[3H]DHA) binding in the rat hypothalamus appears to possess all the characteristics expected of physiologically relevant beta-adrenergic receptors. Binding of (-)-[3H]DHA to the hypothalamic sites was rapid (k1 = 1.3 X 10(-7) min-1) and also rapidly reversible. Binding was saturable at low... | Identification of beta adrenergic receptors in rat hypothalamus. (-)-[3H]-Dihydroalprenolol((-)[3H]DHA) binding in the rat hypothalamus appears to possess all the characteristics expected of physiologically relevant beta-adrenergic receptors. Binding of (-)-[3H]DHA to the hypothalamic sites was rapid (k1 = 1.3 X 10(-7)... | [
-0.058826226741075516,
0.025713235139846802,
-0.03263864293694496,
-0.004724692087620497,
0.008190775290131569,
0.016134275123476982,
0.060504548251628876,
-0.02406379207968712,
-0.013447572477161884,
-0.03603219613432884,
-0.021373029798269272,
-0.055557217448949814,
0.005143825430423021,
... |
PMID:19143 | Resolution of rat renin substrates by isoelectric focusing. | Pooled plasmas from normal or binephrectomized rats and perfusates of isolated livers were used as sources of renin substrate for isoelectric focusing. After desalting, preliminary fractionation (plasma only), and concentration, the preparations were focused in a pH 3--10 gradient on 20-cm glass plates layered with Sep... | Resolution of rat renin substrates by isoelectric focusing. Pooled plasmas from normal or binephrectomized rats and perfusates of isolated livers were used as sources of renin substrate for isoelectric focusing. After desalting, preliminary fractionation (plasma only), and concentration, the preparations were focused i... | [
-0.04385771229863167,
0.01502647902816534,
0.024447711184620857,
-0.005948218051344156,
0.02401890605688095,
-0.029940377920866013,
0.03517519310116768,
0.06412919610738754,
-0.027109771966934204,
0.001565597834996879,
0.013502486981451511,
-0.04365271329879761,
0.02274455316364765,
0.0602... |
PMID:19144 | Preparation and galactosyltransferase acceptor activity of derivatives of antifreeze glycoproteins of an Antarctic fish. | A series of 12 closely related glycoproteins containing alpha-linked N-acetyl-D-galactosamine (GalNAc) as the sole carbohydrate moiety have been prepared by degradation of the antifreeze glycoproteins from the serum of the Antarctic fish Trematomus borchgrevinki. The polypeptide moieties of these glycoproteins contain ... | Preparation and galactosyltransferase acceptor activity of derivatives of antifreeze glycoproteins of an Antarctic fish. A series of 12 closely related glycoproteins containing alpha-linked N-acetyl-D-galactosamine (GalNAc) as the sole carbohydrate moiety have been prepared by degradation of the antifreeze glycoprotein... | [
-0.07325908541679382,
-0.06486643105745316,
0.0030997830908745527,
0.009879960678517818,
0.013482099398970604,
-0.028287477791309357,
0.03373631089925766,
0.00027432956267148256,
-0.07429062575101852,
-0.05867999047040939,
0.0675472617149353,
-0.09605344384908676,
-0.005620580166578293,
0.... |
PMID:19146 | The role of cyclic nucleotides in the CNS. | On the basis of the information presented in this review, it is difficult to reach any firm decision regarding the role of cyclic AMP (or cyclic GMP) in synaptic transmission in the brain. While it is clear that cyclic nucleotide levels can be altered by the exposure of neural tissues to various neurotransmitters, it w... | The role of cyclic nucleotides in the CNS. On the basis of the information presented in this review, it is difficult to reach any firm decision regarding the role of cyclic AMP (or cyclic GMP) in synaptic transmission in the brain. While it is clear that cyclic nucleotide levels can be altered by the exposure of neural... | [
-0.017422152683138847,
-0.017023110762238503,
-0.007834086194634438,
-0.06062735244631767,
-0.002359219593927264,
0.020705746486783028,
0.032277222722768784,
0.03171725943684578,
-0.020699473097920418,
-0.06950372457504272,
0.07357118278741837,
-0.01991753652691841,
-0.017710624262690544,
... |
PMID:19147 | Future of anesthesia. | The authors present an account of some of the problems facing the specialty of anesthesia, which suggests that the interdependence of these problems and of their solutions should be recognized. Recruitment, job satisfaction and clinical standards will be improved as a result of greater involvement by anesthetists in pr... | Future of anesthesia. The authors present an account of some of the problems facing the specialty of anesthesia, which suggests that the interdependence of these problems and of their solutions should be recognized. Recruitment, job satisfaction and clinical standards will be improved as a result of greater involvement... | [
-0.02707235887646675,
0.019048640504479408,
0.05824185907840729,
-0.002962397178635001,
-0.0489170141518116,
0.009195909835398197,
0.029479237273335457,
0.006512089166790247,
-0.03905816376209259,
0.007777239196002483,
0.0026370976120233536,
0.027203815057873726,
-0.03572004288434982,
0.02... |
PMID:19148 | The trauma of being psychotic: a neglected element in the management of chronic schizophrenia? | A hypothesis is put forward in regards to what is called "chronic schizophrenia" that those observations which suggest a continuing disease process may turn out not to be intrinsic facets of schizophrenia as a neurochemical instability but rather neurotic reactions to the acute schizophrenic process. The hypothesis goe... | The trauma of being psychotic: a neglected element in the management of chronic schizophrenia? A hypothesis is put forward in regards to what is called "chronic schizophrenia" that those observations which suggest a continuing disease process may turn out not to be intrinsic facets of schizophrenia as a neurochemical i... | [
-0.005370750091969967,
0.047020770609378815,
0.008011206053197384,
0.0392281673848629,
0.01323391031473875,
0.05790286138653755,
0.08206795901060104,
0.02132726088166237,
-0.03144470602273941,
-0.02573336660861969,
0.039664775133132935,
-0.01049316767603159,
-0.007406259421259165,
0.065104... |
PMID:19149 | Desensitization therapy for body image anxiety. | A newly devised therapy technique to desensitize individuals for body image anxiety arising from conversion symptoms and psychophysiologic disorders is outlined. One application involves the projective use of inkblots with high anatomical content which provide a source of visual input to stimulate somatic images in con... | Desensitization therapy for body image anxiety. A newly devised therapy technique to desensitize individuals for body image anxiety arising from conversion symptoms and psychophysiologic disorders is outlined. One application involves the projective use of inkblots with high anatomical content which provide a source of... | [
0.010401444509625435,
-0.006697646342217922,
0.040877047926187515,
0.016865473240613937,
0.015562822110950947,
0.012287444435060024,
0.05024242773652077,
0.027618324384093285,
0.006381981540471315,
-0.05765166133642197,
0.03832302242517471,
-0.02809523232281208,
-0.02890974096953869,
0.040... |
PMID:19150 | The ability of enteric bacteria to catalyze the covalent binding of bile acids and cholesterol to DNA and their in ability to metabolize benzo(a)pyrene to a binding product and to known metabolites. | The capacity of enteric bacteria (E. coli, Salmonella, Pseudomonas, Shigella and Klebsiella) to catalyze the covalent binding of benzo(a)pyrene (BP), cholic acid, deoxycholic acid and cholesterol was investigated. In general, these bacteria were incapable of activating BP to a covalently bound product with calf thymus ... | The ability of enteric bacteria to catalyze the covalent binding of bile acids and cholesterol to DNA and their in ability to metabolize benzo(a)pyrene to a binding product and to known metabolites. The capacity of enteric bacteria (E. coli, Salmonella, Pseudomonas, Shigella and Klebsiella) to catalyze the covalent bin... | [
-0.012681003659963608,
0.006628495175391436,
-0.04412096366286278,
-0.01367116253823042,
-0.008606375195086002,
-0.048177048563957214,
0.013568757101893425,
0.016817722469568253,
-0.04398399963974953,
-0.029965275898575783,
0.020036572590470314,
-0.10340578854084015,
0.0011964450823143125,
... |
PMID:19152 | Beta-adrenoceptor antagonists and the release of creatine phosphokinase from hypoxic heart muscle. | The ability of several beta-adrenoceptor antagonists with partial agonist activity (dl-oxprenolol, dl-acebutolol and dl-practolol) to attenuate the release of CPK that occurs during hypoxia (pO2 less than 0.8 kPa [6 MMHg]) has been studied and compared with the protection provided by dl-propranolol. dl-propranolol atte... | Beta-adrenoceptor antagonists and the release of creatine phosphokinase from hypoxic heart muscle. The ability of several beta-adrenoceptor antagonists with partial agonist activity (dl-oxprenolol, dl-acebutolol and dl-practolol) to attenuate the release of CPK that occurs during hypoxia (pO2 less than 0.8 kPa [6 MMHg]... | [
-0.07894288748502731,
0.04480930417776108,
-0.01034175232052803,
0.025435086339712143,
-0.04754213988780975,
-0.00819141510874033,
-0.0026608482003211975,
0.020061761140823364,
-0.04247443005442619,
-0.03340950608253479,
0.014103017747402191,
-0.009608824737370014,
-0.0073507907800376415,
... |
PMID:19154 | The nature of the rate-limiting step in aniline hydroxylation involving cytochrome p-450 rat liver microsomes. | The kinetics of aniline hydroxylation was studied with: (1) rat liver microsomes involving NADPH and O2 (system 1), (2) hepatic microsomes and tert-butylhydroperoxide (system 2) and (3) microsomes and cumyl hydroperoxide (system 3) at 15--37 degrees C. The reactions were characterized by the values of the aniline oxida... | The nature of the rate-limiting step in aniline hydroxylation involving cytochrome p-450 rat liver microsomes. The kinetics of aniline hydroxylation was studied with: (1) rat liver microsomes involving NADPH and O2 (system 1), (2) hepatic microsomes and tert-butylhydroperoxide (system 2) and (3) microsomes and cumyl hy... | [
-0.08752771466970444,
0.034380391240119934,
-0.037805695086717606,
-0.009132233448326588,
-0.012756556272506714,
0.0038029984571039677,
0.022319963201880455,
-0.02974458783864975,
-0.002673768438398838,
0.03681189939379692,
0.021449018269777298,
-0.01186369638890028,
-0.015995174646377563,
... |
PMID:19157 | [Splenogonadal fusion]. | The authors report of case of splenogonadic fusion in abdominal retention, revising the subject from the aetiopathogenetic and diagnostic point of view. It is emphasized that correct recognition of the lesion can obviate useless orchiectomies, preserving normal function of the testicle at least from the endocrinal stan... | [Splenogonadal fusion]. The authors report of case of splenogonadic fusion in abdominal retention, revising the subject from the aetiopathogenetic and diagnostic point of view. It is emphasized that correct recognition of the lesion can obviate useless orchiectomies, preserving normal function of the testicle at least ... | [
0.047259505838155746,
0.005443958565592766,
0.0089390454813838,
0.030719218775629997,
-0.005656563676893711,
0.011096455156803131,
0.08065317571163177,
0.06500519812107086,
-0.010137270204722881,
-0.005219441372901201,
0.028360895812511444,
-0.04732383415102959,
0.040855951607227325,
0.024... |
PMID:19160 | [Properties of chlorotetrolic acid and its ester as possible protein acetylenic reagents]. | Chlorotetrolic acid and, even better, the corresponding methyl ester react under mild conditions with different functional groups. The relative ease with which the same nucleophile adds to the triple bond and substitutes the chlorine was studied and the stability of the addition products was compared. | [Properties of chlorotetrolic acid and its ester as possible protein acetylenic reagents]. Chlorotetrolic acid and, even better, the corresponding methyl ester react under mild conditions with different functional groups. The relative ease with which the same nucleophile adds to the triple bond and substitutes the chlo... | [
-0.09907612204551697,
0.02099921554327011,
-0.051811475306749344,
0.027140356600284576,
0.03098616562783718,
0.0027928296476602554,
0.06567981839179993,
0.0497334748506546,
-0.07467188686132431,
-0.012726391665637493,
0.017264999449253082,
-0.06574934720993042,
0.0026215496473014355,
-0.08... |
PMID:19163 | Nucleotide pyrophosphatase and phosphodiesterase. I. Organ distribution and activities in body fluids. | We estimated nucleotide pyrophosphatase and phosphodiesterase I activities in human and rat organs and in body fluids from man and dog. The highest organ activities were found in epididymis, kidney, liver, and intestine. In body fluids, the activity was highest in seminal plasma, followed by intestinal lymph, serum, he... | Nucleotide pyrophosphatase and phosphodiesterase. I. Organ distribution and activities in body fluids. We estimated nucleotide pyrophosphatase and phosphodiesterase I activities in human and rat organs and in body fluids from man and dog. The highest organ activities were found in epididymis, kidney, liver, and intesti... | [
-0.008359801024198532,
-0.004557951353490353,
-0.00774193974211812,
-0.03529681637883186,
0.014712067320942879,
-0.038094259798526764,
0.10840213298797607,
0.05637936294078827,
-0.03408357873558998,
-0.03209638595581055,
0.007357585243880749,
-0.05146695673465729,
-0.0690918117761612,
0.00... |
Subsets and Splits
No community queries yet
The top public SQL queries from the community will appear here once available.