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10. 1002/app. 53593 | 2,023 | Journal of Applied Polymer Science | Extrusion | Abstract Extrusion printing represents one of the leading additive manufacturing techniques for tissue engineering purposes due to the possibility of achieving accurate control of the final shape and porosity of the scaffold. Despite many polymeric materials having already been optimized for this application, the proce... | 1 INTRODUCTION In recent decades, tissue engineering is emerging as a strategic alternative to meet the growing demand for rapid and effective tissue regeneration following injuries and pathological conditions. The correct choice of biologically inspired biomaterials and fabrication processes is therefore considered a ... |
10. 1002/app. 54242 | 2,023 | Journal of Applied Polymer Science | Effects of electrospun fibers containing ascorbic acid on oxidative stress reduction for cardiac tissue engineering | Abstract Tissue engineering provides promise for regeneration of cardiac tissue following myocardial infarction. However, the harsh microenvironment of the infarct hampers the efficacy of regenerative therapies. Ischemia‐reperfusion injury dramatically increases the levels of reactive oxygen species (ROS) within the in... | 1 INTRODUCTION Cardiovascular diseases (CVDs) remain the leading cause of death worldwide; it is estimated that over 9 million people die from ischemic heart diseases globally, accounting for 16% of all deaths. 1 The blockage of a major coronary artery results in myocardial infarction, characterized by a loss of large ... |
10. 1002/ar. 23918 | 2,018 | Anatomical Record (Hoboken, N. j. : 2007) | Developing an | ABSTRACT Peripheral nerve injuries (PNI) have a high prevalence and can be debilitating, resulting in life‐long loss or disturbance in end‐organ function, which compromises quality of life for patients. Current therapies use microsurgical approaches but there is the potential for enhancing recovery through other therap... | Damage to the peripheral nervous system can be debilitating and result in loss of motor and sensory function, coupled with poor neuron regeneration capacity (Hoke and Brushart, 2010 ). Despite increased understanding of the complex neuropathophysiology following such an injury, the principal clinical treatments have no... |
10. 1002/ar. 23919 | 2,018 | Anatomical Record (Hoboken, N. j. : 2007) | Vascularization Strategies for Peripheral Nerve Tissue Engineering | ABSTRACT Vascularization plays a significant role in treating nerve injury, especially to avoid the central necrosis observed in nerve grafts for large and long nerve defects. It is known that sufficient vascularization can sustain cell survival and maintain cell integration within tissue‐engineered constructs. Several... | ROLE OF BLOOD VESSELS AND ENDOTHELIAL CELLS IN GUIDING NERVE REGENERATION The vasculature plays a fundamental role in supporting the function of peripheral nerves through its supply of blood, oxygen and other nutrients to the cells comprising nerve tissue. Within the body, most cells reside within around 100–200 μm fro... |
10. 1002/ar. 24067 | 2,019 | Anatomical Record (Hoboken, N. j. : 2007) | Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology | ABSTRACT In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibilit... | Stem cells differ from other cells in the body. In general, stem cells have a few unique characteristics over somatic cells; they are undifferentiated and can give rise to differentiated daughter cells and some stem cells have the ability to divide and renew above the Hayflick limit of somatic cells (Ding and Schultz, ... |
10. 1002/art. 39982 | 2,016 | Arthritis & rheumatology (Hoboken, N. J. ) | CRISPR/Cas9 editing of induced pluripotent stem cells for engineering inflammation-resistant tissues | Objective Pro-inflammatory cytokines such as interleukin 1 (IL-1) are elevated in diseased or injured tissues and promote rapid tissue degradation while preventing stem cell differentiation. The goals of this study were to engineer inflammation-resistant induced pluripotent stem cells (iPSCs) through deletion of the IL... | No full text available |
10. 1002/ase. 2171 | 2,022 | Anatomical Sciences Education | Identification of histological threshold concepts in health sciences curricula: Students' perception | Abstract Students' metacognitive skills and perceptions are considered important variables for high‐quality learning. In this study, students' perceptions were used to identify histological threshold concepts (integrative, irreversible, transformative, and troublesome) in three health sciences curricula. A specific que... | INTRODUCTION The identification of students' perceptions constitutes a crucial element for the appropriate design and implementation of pedagogical strategies. Given that the learning process depends on triggers such as motivation (Wouters et al. , 2016 ), self‐recognition of progress (Berkovich‐Ohana et al. , 2019 ), ... |
10. 1002/asia. 202200201 | 2,022 | Chemistry, an Asian Journal | Strategies to Improve the Potency of Oxazolidinones towards Bacterial Biofilms | Abstract Biofilms are part of the natural lifecycle of bacteria and are known to cause chronic infections that are difficult to treat. Most antibiotics are developed and tested against bacteria in the planktonic state and are ineffective against bacterial biofilms. The oxazolidinones, including the last resort drug lin... | 1 Introduction 1. 1 Infectious diseases and biofilms Infectious diseases are a major cause of mortality worldwide and it is estimated that 17 million people die each year from bacterial infections. Biofilm associated infections are particularly problematic because they are a significant contributor to bacterial pathoge... |
10. 1002/asia. 202200797 | 2,022 | Chemistry, an Asian Journal | Fabrication Strategies Towards Hydrogels for Biomedical Application: Chemical and Mechanical Insights | Abstract This review aims at giving selected chemical and mechanical insights on design criteria that should be taken into account in hydrogel production for biomedical applications. Particular emphasis will be given to the chemical aspects involved in hydrogel design: macromer chemical composition, cross‐linking strat... | 1 Introduction Hydrogels can be defined as three‐dimensional polymeric networks able to swell and absorb high amounts of water without dissolving in short times. Cross links among the polymer macromers used to create the gel network are usually needed, to provide resistance to degradation in water and to impart mechani... |
10. 1002/bab. 2245 | 2,021 | Biotechnology and Applied Biochemistry | 3D‐printed scaffold composites for the stimuli‐induced local delivery of bioactive adjuncts | Abstract Polysaccharide scaffolds have been successfully employed to reconstruct environments that sustain skin tissue regeneration after injuries. Three‐dimensional (3D) advanced additive manufacturing technologies allow creating scaffolds with controlled and reproducible macro‐ and micro‐structure that improve the qu... | Abbreviations 3D three dimensional AL alginate CH chitosan EGF epidermal growth factor HELP human elastin‐like polypeptide SDS‐PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis 1 INTRODUCTION Every organism has the intrinsic capacity to restore biological structures and functions after injuries. Skin tissu... |
10. 1002/bco2. 327 | 2,024 | BJUI Compass | Newly modified ‘pseudo flap’ without compromising vascularity to enhance repair of long distal ureteral loss: A retrospective analysis of a prospective database | Abstract Objective To present an alternative technique called pseudo‐flap for reconstructing long ureteral defects as an alternative to Boari flap. Despite being used for more than 70 years by urologists for tension‐free reconstruction of distal and mid‐ureteral defects, the Boari flap exhibits high complication rates,... | 1 INTRODUCTION Ureteral injuries have always been challenging to the surgical team. Based on the multitude of primary aetiologies, the length and the location of the ureteric loss, management of ureteral injuries can be a dilemma. Because longer and more proximal ureteral segments cannot be merely repaired with end‐to‐... |
10. 1002/bdr2. 1345 | 2,018 | Birth defects research | The Role of 3D-Printing in Treating Craniomaxillofacial Congenital Anomalies | Craniomaxillofacial congenital anomalies comprise approximately one third of all congenital birth defects and include deformities such as alveolar clefts, craniosynostosis, and microtia. Current surgical treatments commonly require the use of autogenous graft material which are difficult to shape, limited in supply, as... | No full text available |
10. 1002/bdr2. 1533 | 2,019 | Birth Defects Research | Regenerative robotics | Abstract Congenital diseases requiring reconstruction of parts of the gastrointestinal tract, skin, or bone are a challenge to alleviate especially in rapidly growing children. Novel technologies may be the answer. This article presents the state‐of‐art in regenerative robotic technologies, which are technologies that ... | 1 TISSUE REPAIR Tissue repair is a complex, long term, and physiologically demanding process requiring dynamic and optimal therapies (Eming, Wynn, & Martin, 2017 ). Patients suffering from conditions such as long‐gap esophageal atresia (LGEA), a congenital disease in which a section of the esophagus of 3 cm or more is ... |
10. 1002/bdrc. 21075 | 2,015 | Birth defects research. Part C, Embryo today : reviews | Cranial Neural Crest Cell Contribution to Craniofacial Formation, Pathology, and Future Directions in Tissue Engineering | This review provides an overview of the state and future directions of development and pathology in the craniofacial complex in the context of Cranial Neural Crest Cells (CNCC). CNCC are a multipotent cell population that is largely responsible for forming the vertebrate head. We focus on findings that have increased t... | No full text available |
10. 1002/bem. 22401 | 2,022 | Bioelectromagnetics | Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage | Rapid advances in mesenchymal stem progenitor cells (MSPCs) have rendered impetus into the area of cell therapy and regenerative medicine. The main promise of future stem cell therapies is their reliance on autologous stem cells derived from adipose tissue, which also includes treatments of bone fractures and degenerat... | INTRODUCTION Osteoporosis and osteoporosis‐related fractures are becoming the most prevalent degenerative bone diseases within the aging population. More than 75 million people suffer from osteoporosis in the United States, the European Union, and Japan [Bicer et al. , 2021 ], and it is anticipated that by 2050 the num... |
10. 1002/bies. 201600104 | 2,016 | Bioessays | Peptide drugs accelerate BMP‐2‐induced calvarial bone regeneration and stimulate osteoblast differentiation through mTORC1 signaling | Both W9 and OP3‐4 were known to bind the receptor activator of NF‐κB ligand (RANKL), inhibiting osteoclastogenesis. Recently, both peptides were shown to stimulate osteoblast differentiation; however, the mechanism underlying the activity of these peptides remains to be clarified. A primary osteoblast culture showed th... | Introduction Bone morphogenetic protein (BMP)‐2 is known to induce local bone formation and has been used clinically 1. However, recent evidence suggests that BMP‐2 has the potential to cause cancer and inflammation 2, 3, 4. In this context, a reduction in the amount of BMP‐2 that is used in BMP‐2 therapy is necessary ... |
10. 1002/biot. 201300187 | 2,015 | Biotechnology journal | Physiologically relevant organs on chips | Recent advances in integrating microengineering and tissue engineering have generated promising microengineered physiological models for experimental medicine and pharmaceutical research. Here we review the recent development of microengineered physiological systems, or organs on chips, that reconstitute the physiologi... | No full text available |
10. 1002/biot. 201300302 | 2,013 | Biotechnology Journal | Spatial organization of cell-adhesive ligands for advanced cell culture | Interaction between biomaterials and cells is a critical aspect for successful application of tissue engineering research. Technological advances within the past decade have enabled a number of studies to investigate how the spatial organization of cell-adhesive ligands impacts complex and rich cell behaviors ranging f... | Abbreviations: E-beam, electron beam lithography; ECM, extracellular matrix; MSC, mesenchymal stem cell; PDMS, poly(dimethyl siloxane); PEG (PEO), polyethylene glycol (polyethylene oxide); PS, polystyrene; RGD, arginine-glycine-aspartic acid peptide sequence 1 Introduction The engineering of biomaterial surfaces and ce... |
10. 1002/biot. 201400813 | 2,015 | Biotechnology Journal | A perfusion bioreactor system efficiently generates cell‐loaded bone substitute materials for addressing critical size bone defects | Abstract Critical size bone defects and non‐union fractions are still challenging to treat. Cell‐loaded bone substitutes have shown improved bone ingrowth and bone formation. However, a lack of methods for homogenously colonizing scaffolds limits the maximum volume of bone grafts. Additionally, therapy robustness is im... | Abbreviations: CAD, computer‐aided design; DMEM, Dulbecco's Modified Eagle's Medium; FACS, fluorescence activated cell sorting; FCS, fetal calf serum; g 0, standard acceleration due to gravity; hMSCs, human mesenchymal stem cells; MTT, 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐Diphenyltetrazolium bromide; P(LLA‐co‐CL), poly(L... |
10. 1002/biot. 201400862 | 2,016 | Biotechnology Journal | Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors | Abstract Production of human mesenchymal stem cells for allogeneic cell therapies requires scalable, cost‐effective manufacturing processes. Microcarriers enable the culture of anchorage‐dependent cells in stirred‐tank bioreactors. However, no robust, transferable methodology for microcarrier selection exists, with stu... | Abbreviations DMEM Dulbecco's Modified Eagle Medium FBS foetal bovine serum hMSC human mesenchymal stem cell hBM‐MSC human bone marrow‐derived mesenchymal stem cell ISCT International Society for Cellular Therapy PLGA poly(lactic‐co‐glycolic acid) 1 Introduction Pioneering work in the 1970s led to the isolation and ide... |
10. 1002/biot. 201600326 | 2,016 | Biotechnology Journal | A versatile modular bioreactor platform for Tissue Engineering | Abstract Tissue Engineering (TE) bears potential to overcome the persistent shortage of donor organs in transplantation medicine. Additionally, TE products are applied as human test systems in pharmaceutical research to close the gap between animal testing and the administration of drugs to human subjects in clinical t... | Abbreviations acLDL acetylated low‐density lipoprotein αSMA alpha smooth muscle actin CAD computer‐aided design CD31 cluster of differentiation 31 H&E hematoxylin and eosin MEM minimum essential medium PCK pan cytokeratin PID proportional/integral/differential SIS small intestinal submucosa TE tissue engineering USB un... |
10. 1002/biot. 201600750 | 2,017 | Biotechnology journal | Mechanotransduction Effects on Endothelial Cell Proliferation via CD31 and VEGFR2: Implications for Immunomagnetic Separation | Immunomagnetic separation is used to isolate circulating endothelial cells (ECs) and endothelial progenitor cells (EPCs) for diagnostics and tissue engineering. However, potentially detrimental changes in cell properties have been observed post-separation. Here, we studied the effect of mechanical force, which is natur... | No full text available |
10. 1002/biot. 201700070 | 2,017 | Biotechnology Journal | Mesenchymal Stem Cells: Potential Role in the Treatment of Osteochondral Lesions of the Ankle | Given articular cartilage has a limited repair potential, untreated osteochondral lesions of the ankle can lead to debilitating symptoms and joint deterioration necessitating joint replacement. While a wide range of reparative and restorative surgical techniques have been developed to treat osteochondral lesions of the... | 1 Introduction Hyaline articular cartilage (AC) covers the ends of articulating bones and its unique biomechanical characteristics reduce friction during bone movement by facilitating adsorption of mechanical load. This complex tissue is avascular, aneural, alymphatic, and is sparsely populated with chondrocytes that p... |
10. 1002/biot. 201700703 | 2,019 | Biotechnology journal | Sacrificial Bioprinting of A Mammary Ductal Carcinoma Model | Cancer tissue engineering has remained challenging due to the limitation of the conventional biofabrication techniques to model the complex cancer microenvironment. Here we report the utilization of a sacrificial bioprinting strategy to generate biomimetic mammary duct-like structures within a hydrogel matrix, which wa... | No full text available |
10. 1002/biot. 201700763 | 2,019 | Biotechnology journal | Exogenous lysyl oxidase-like 2 and perfusion culture induce collagen crosslink formation in osteogenic grafts | Lysyl oxidase (LOX)-mediated collagen crosslinking can regulate osteoblastic phenotype and enhance mechanical properties of tissues, both areas of interest in bone tissue engineering. The objective of this study was to investigate the effect of lysyl oxidase-like 2 (LOXL2) on osteogenic differentiation of mesenchymal s... | No full text available |
10. 1002/bip. 22412 | 2,014 | Biopolymers | Antimicrobial hydrogels for the treatment of infection | The increasing prevalence of microbial infections, especially those associated with impaired wound healing and biomedical implant failure has spurred the development of new materials having antimicrobial activity. Hydrogels are a class of highly hydrated material finding use in diverse medical applications such as drug... | No full text available |
10. 1002/bip. 22486 | 2,014 | Biopolymers | Collagen-Based Biomaterials for Wound Healing | With its wide distribution in soft and hard connective tissues, collagen is the most abundant of animal proteins. In vitro, natural collagen can be formed into highly organized, three-dimensional scaffolds that are intrinsically biocompatible, biodegradable, non-toxic upon exogenous application, and endowed with high t... | No full text available |
10. 1002/bip. 23078 | 2,017 | Biopolymers | Design and development of low cost polyurethane biopolymer based on castor oil and glycerol for biomedical applications | Abstract In the current study, we present the synthesis of novel low cost bio‐polyurethane compositions with variable mechanical properties based on castor oil and glycerol for biomedical applications. A detailed investigation of the physicochemical properties of the polymer was carried out by using mechanical testing,... | 1 INTRODUCTION Polyurethanes (PU) are the most common synthetic polymers used for various medical devices and implants. 1 They are used in the production of medical devices and implants such as catheters, heart valves, cardiovascular devices, and artificial organs. 2 This is possible because of their excellent structur... |
10. 1002/bip. 23232 | 2,019 | Biopolymers | Storage stability of electrospun pure gelatin stabilized with EDC/Sulfo-NHS | With the rapid development of biomimetic polymers for cell-based assays and tissue engineering, crosslinking electrospun nanofibrous biopolymer constructs is of great importance for achieving sustainable and efficient 3D scaffold constructs. Uncrosslinked electrospun gelatin nanofibrous constructs immediately and compl... | No full text available |
10. 1002/bip. 23527 | 2,022 | Biopolymers | Advances in biomimetic collagen mineralisation and future approaches to bone tissue engineering | Abstract With an ageing world population and ~20% of adults in Europe being affected by bone diseases, there is an urgent need to develop advanced regenerative approaches and biomaterials capable to facilitate tissue regeneration while providing an adequate microenvironment for cells to thrive. As the main components o... | 1 INTRODUCTION Most conventional strategies for the repair of damaged bone incur further damage to already compromised tissues throughout their use. For instance, the implant site must be reshaped via the removal of bone tissue to accommodate a standardised component, this occurs again for each revision surgery require... |
10. 1002/bit. 24957 | 2,013 | Biotechnology and bioengineering | Digital microfabrication of user-defined 3D microstructures in cell-laden hydrogels | Complex 3D interfacial arrangements of cells are found in several in vivo biosystems such as blood vasculature, renal glomeruli, and intestinal villi. Current tissue engineering techniques fail to develop suitable 3D microenvironments to evaluate the concurrent effects of complex topography and cell encapsulation. Ther... | No full text available |
10. 1002/bit. 24968 | 2,014 | Biotechnology and bioengineering | Improving efficiency of human pluripotent stem cell differentiation platforms using an integrated experimental and computational approach | Human pluripotent stem cells (hPSCs) have an unparalleled potential for tissue engineering applications including regenerative therapies and in vitro cell-based models for studying normal and diseased tissue morphogenesis, or drug and toxicological screens. While numerous hPSC differentiation methods have been develope... | No full text available |
10. 1002/bit. 24995 | 2,013 | Biotechnology and bioengineering | The influence of electrospun scaffold topography on endothelial cell morphology, alignment, and adhesion in response to fluid flow | Bioengineered vascular grafts provide a promising alternative to autografts for replacing diseased or damaged arteries, but necessitate scaffold designs capable of supporting a confluent endothelium that resists endothelial cell (EC) detachment under fluid flow. To this end, we investigated whether tuning electrospun t... | No full text available |
10. 1002/bit. 25175 | 2,014 | Biotechnology and bioengineering | Lentivirus Delivery of IL-10 to Promote and Sustain Macrophage Polarization Towards an Anti-Inflammatory Phenotype | Gene delivery from biomaterials can create an environment that promotes and guides tissue formation. However, the immune response induced upon biomaterial implantation can be detrimental to tissue regeneration. Macrophages play a central role in mediating early phases of this response, and functional “polarization” of ... | No full text available |
10. 1002/bit. 25179 | 2,014 | Biotechnology and Bioengineering | Cell cultivation under different gravitational loads using a novel random positioning incubator | Important in biotechnology is the establishment of cell culture methods that reflect the in vivo situation accurately. One approach for reaching this goal is through 3D cell cultivation that mimics tissue or organ structures and functions. We present here a newly designed and constructed random positioning incubator (R... | Introduction Recent tissue engineering studies have revealed striking discrepancies between the behavior of cells cultured in 3D and 2D, where 3D cell cultures mimicked intact tissue in terms of viability and gene expression (Baharvand et al. , 2006 ; Sun et al. , 2006 ). Indeed, 3D cell culture is one favorable approa... |
10. 1002/bit. 25241 | 2,014 | Biotechnology and Bioengineering | The effect of oxygen tension on human articular chondrocyte matrix synthesis: Integration of experimental and computational approaches | Significant oxygen gradients occur within tissue engineered cartilaginous constructs. Although oxygen tension is an important limiting parameter in the development of new cartilage matrix, its precise role in matrix formation by chondrocytes remains controversial, primarily due to discrepancies in the experimental setu... | Introduction The limited capacity of adult articular cartilage to self-repair has led to considerable interest in the development of effective cell-based therapies and cartilage tissue engineering strategies to treat cartilage defects in an increasing aging population. The chondrogenic potential of articular chondrocyt... |
10. 1002/bit. 25291 | 2,015 | Biotechnology and bioengineering | Effects of shear stress pattern and magnitude on mesenchymal transformation and invasion of aortic valve endothelial cells | Understanding the role of mechanical forces on cell behavior is critical for tissue engineering, regenerative medicine, and disease initiation studies. Current hemodynamic bioreactors are largely limited to 2D substrates or the application of general flow conditions at a tissue level, which eliminates the investigation... | No full text available |
10. 1002/bit. 25987 | 2,022 | Biotechnology and bioengineering | Microcapsules and 3D Customizable Shelled Microenvironments From Laser Direct-Written Microbeads | Microcapsules are shelled 3D microenvironments, with a liquid core. These core-shelled structures enable cell–cell contact, cellular proliferation and aggregation within the capsule, and can be utilized for controlled release of encapsulated contents. Traditional microcapsule fabrication methods provide limited control... | No full text available |
10. 1002/bit. 26202 | 2,016 | Biotechnology and Bioengineering | Oxygen mapping: Probing a novel seeding strategy for bone tissue engineering | ABSTRACT Bone tissue engineering (BTE) utilizing biomaterial scaffolds and human mesenchymal stem cells (hMSCs) is a promising approach for the treatment of bone defects. The quality of engineered tissue is crucially affected by numerous parameters including cell density and the oxygen supply. In this study, a novel ox... | Introduction Optimal treatment of bone fractures and bone diseases is of pivotal importance in clinical‐experimental research (Ceccarelli et al. , 2013 ). To date, bone replacement grafts are seen as the gold standard and have an established role in the treatment of various bone diseases like tumor resections, traumati... |
10. 1002/bit. 26320 | 2,017 | Biotechnology and bioengineering | Characterization of the chondrocyte secretome in photoclickable poly(ethylene glycol) hydrogels | Poly(ethylene glycol) (PEG) hydrogels are highly tunable platforms that are promising cell delivery vehicles for chondrocytes and cartilage tissue engineering. In addition to characterizing the type of extracellular matrix (ECM) that forms, understanding the types of proteins that are secreted by encapsulated cells may... | No full text available |
10. 1002/bit. 26370 | 2,017 | Biotechnology and Bioengineering | Integrated gut/liver microphysiological systems elucidates inflammatory inter‐tissue crosstalk | ABSTRACT A capability for analyzing complex cellular communication among tissues is important in drug discovery and development, and in vitro technologies for doing so are required for human applications. A prominent instance is communication between the gut and the liver, whereby perturbations of one tissue can influe... | Introduction In drug discovery and development, animal models have been valuable for dissecting some biological mechanisms, but they are inadequate for recapitulating polygenic and multifactorial human diseases with diverse clinical phenotypes. As a result, drug attrition in Phase II and III clinical trials is now attr... |
10. 1002/bit. 26452 | 2,017 | Biotechnology and bioengineering | Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography | Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-s... | No full text available |
10. 1002/bit. 26514 | 2,018 | Biotechnology and bioengineering | Fabrication and evaluation of 3D printed BCP scaffolds reinforced with ZrO | Fused deposition modeling (FDM) is a promising 3D printing and manufacturing step to create well interconnected porous scaffold designs from the computer-aided design (CAD) models for the next generation of bone scaffolds. The purpose of this study was to fabricate and evaluate a new biphasic calcium phosphate (BCP) sc... | No full text available |
10. 1002/bit. 26778 | 2,018 | Biotechnology and bioengineering | Vascularized Microfluidic Platforms to Mimic the Tumor Microenvironment | Microfluidic technology has led to the development of advanced in vitro tumor platforms that overcome the challenges of in vivo animal and in vitro two dimensional models. This paper presents platform designs and methods used to develop complex vascularized in vitro models to mimic the tumor microenvironment. Features ... | No full text available |
10. 1002/bit. 26784 | 2,018 | Biotechnology and Bioengineering | Hepatocyte‐like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers | Abstract Direct reprogramming represents an easy technique to generate induced hepatocytes (iHeps) from somatic cells. However, current protocols are accompanied by several drawbacks as iHeps are heterogenous and lack fully mature phenotypes of primary hepatocytes. Here, we established a polycistronic expression system... | 1 INTRODUCTION Currently, orthotopic liver transplantation is the only cure for severe acute and chronic liver diseases. Unfortunately, the shortage of donor livers is a growing problem. In Europe alone, 22% of the patients die while on the waiting list for a liver transplant (Brangel & Undine, 2016 ). Therefore, the d... |
10. 1002/bit. 26801 | 2,020 | Biotechnology and bioengineering | Hierarchically aligned fibrous hydrogel films through microfluidic self-assembly of graphene and polysaccharides | Despite significant interest in developing extracellular matrix (ECM)-inspired biomaterials to recreate native cell-instructive microenvironments, the major challenge in the biomaterial field is to recapitulate the complex structural and biophysical features of native ECM. These biophysical features include multiscale ... | No full text available |
10. 1002/bit. 26860 | 2,018 | Biotechnology and bioengineering | Assessing the ability of human endothelial cells derived from induced pluripotent stem cells to form functional microvasculature i | Forming functional blood vessel networks is a major clinical challenge in the fields of tissue engineering and therapeutic angiogenesis. Cell-based strategies to promote neovascularization have been widely explored, but cell sourcing remains a significant limitation. Induced pluripotent stem cell-derived endothelial ce... | No full text available |
10. 1002/bit. 26870 | 2,018 | Biotechnology and Bioengineering | Mechanisms of unprimed and dexamethasone‐primed nonviral gene delivery to human mesenchymal stem cells | Abstract Human mesenchymal stem cells (hMSCs) are under intense study for applications of cell and gene therapeutics because of their unique immunomodulatory and regenerative properties. Safe and efficient genetic modification of hMSCs could increase their clinical potential by allowing functional expression of therape... | 1 INTRODUCTION Because of their unique roles in wound healing (Chamberlain, Fox, Ashton, & Middleton, 2007 ), trophic tissue support (Maxson, Lopez, Yoo, Danilkovitch‐Miagkova, & Leroux, 2012 ; Singer & Caplan, 2011 ), and immunomodulation (Ren et al. , 2008 ), along with their differentiation ability (Baksh, Song, & T... |
10. 1002/bit. 27024 | 2,019 | Biotechnology and Bioengineering | Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring | Abstract The new and rapid advancement in the complexity of biologics drug discovery has been driven by a deeper understanding of biological systems combined with innovative new therapeutic modalities, paving the way to breakthrough therapies for previously intractable diseases. These exciting times in biomedical innov... | 1 INTRODUCTION OF LIGHT‐INDUCED ELECTROKINETICS AND IMPACT ON BIOLOGICS DISCOVERY A new generation of techniques based on forces exerted by a light beam (known as optical manipulations) is enabling interactive biology at the cellular level, thus opening new opportunities in drug discovery. Optical manipulation—which en... |
10. 1002/bit. 27127 | 2,019 | Biotechnology and Bioengineering | Osteochondral tissue coculture: An in vitro and in silico approach | Abstract Osteochondral tissue engineering aims to regenerate functional tissue‐mimicking physiological properties of injured cartilage and its subchondral bone. Given the distinct structural and biochemical difference between bone and cartilage, bilayered scaffolds, and bioreactors are commonly employed. We present an ... | 1 INTRODUCTION Osteoarthritis of the synovial joint is a common cause of osteochondral defects. Osteoarthritis of the knee accounts for 83% of total osteoarthritis burden and affects around 250 million people globally (Vos et al. , 2012 ). Injured cartilage does not heal spontaneously due to limited access to progenito... |
10. 1002/bit. 27164 | 2,019 | Biotechnology and bioengineering | Dual-Chambered Membrane Bioreactor for Co-Culture of Stratified Cell Populations | We have developed a dual-chambered bioreactor (DCB) that incorporates a membrane to study stratified 3D cell populations for skin tissue engineering. The DCB provides adjacent flow lines within a common chamber; the inclusion of the membrane regulates flow layering or mixing, which can be exploited to produce layers or... | No full text available |
10. 1002/bit. 27167 | 2,019 | Biotechnology and Bioengineering | Albumin‐based hydrogels for regenerative engineering and cell transplantation | Abstract Albumin, the most abundant plasma protein in mammals, is a versatile and easily obtainable biomaterial. It is pH and temperature responsive, dissolvable in high concentrations and gels readily in defined conditions. This versatility, together with its inexpensiveness and biocompatibility, makes albumin an attr... | 1 INTRODUCTION Albumin, an endogenous, nonglycosylated protein, is produced predominantly in the liver by hepatocytes and secreted into the blood as a major constituent of plasma. It is comprised of 585 amino acids, has a molecular weight of 66. 4 kDa, and an iso‐electric point of pH 4. 7 (Vlasova & Saletsky, 2009 ). I... |
10. 1002/bit. 27171 | 2,019 | Biotechnology and Bioengineering | Strategy for achieving standardized bone models | Abstract Reliably producing functional in vitro organ models, such as organ‐on‐chip systems, has the potential to considerably advance biology research, drug development time, and resource efficiency. However, despite the ongoing major progress in the field, three‐dimensional bone tissue models remain elusive. In this ... | 1 INTRODUCTION Animal models remain the current gold standard in preclinical drug screening and proof‐of‐concept studies for innovative treatments, but differences in physiology and metabolism result in low transferability rates for human applications (Scheinpflug et al. , 2018 ). However, major advances in tissue engi... |
10. 1002/bit. 27238 | 2,019 | Biotechnology and Bioengineering | Computational fluid dynamic analysis of bioprinted self‐supporting perfused tissue models | Abstract Natural tissues are incorporated with vasculature, which is further integrated with a cardiovascular system responsible for driving perfusion of nutrient‐rich oxygenated blood through the vasculature to support cell metabolism within most cell‐dense tissues. Since scaffold‐free biofabricated tissues being deve... | Abbreviations CFD computational fluid dynamics ECM extracellular matrix FBS fetal bovine serum SSuPer self‐supporting perfused 1 INTRODUCTION The ability to use biofabrication to accurately recreate natural tissues and replicate their in situ environment in vitro with bioreactors will lead to tissues which can (a) serv... |
10. 1002/bit. 27546 | 2,020 | Biotechnology and bioengineering | Development of liver microtissues with functional biliary ductular network | Liver tissue engineering aims to create transplantable liver grafts that can serve as substitutes for donor’s livers. One major challenge in creating a fully functional liver tissue has been to recreate the biliary drainage in an engineered liver construct through integration of bile canaliculi (BC) with the biliary du... | No full text available |
10. 1002/bit. 27613 | 2,020 | Biotechnology and Bioengineering | Scaffolds obtained from decellularized human extrahepatic bile ducts support organoids to establish functional biliary tissue in a dish | Abstract Biliary disorders can lead to life‐threatening disease and are also a challenging complication of liver transplantation. As there are limited treatment options, tissue engineered bile ducts could be employed to replace or repair damaged bile ducts. We explored how these constructs can be created by seeding hep... | 1 INTRODUCTION Biliary complications, such as bile leaks and anastomotic strictures, occurring after donation after circulatory death (DCD) liver transplantation (LT) are common and a major cause of posttransplant complications and morbidity (Hessheimer et al. , 2016 ; de Vries et al. , 2018 ). In DCD LT, the biliary e... |
10. 1002/bit. 27647 | 2,020 | Biotechnology and Bioengineering | High‐ and low‐molecular‐weight chitosan act as adjuvants during single‐dose influenza A virus protein vaccination through distinct mechanisms | Abstract The investigation of new adjuvants is essential for the development of efficacious vaccines. Chitosan (CS), a derivative of chitin, has been shown to act as an adjuvant, improving vaccine‐induced immune responses. However, the effect of CS molecular weight (MW) on this adjuvanticity has not been investigated, ... | 1 INTRODUCTION Adjuvants have been used for over 85 years to improve the immunogenicity of vaccines (Coffman et al. , 2010 ; Di Pasquale et al. , 2015 ; McKee et al. , 2010 ). Adjuvants are used to enhance innate and adaptive immune responses induced during vaccination, allowing for the development of effective memory ... |
10. 1002/bit. 27836 | 2,021 | Biotechnology and Bioengineering | Assessing the influence of perfusion on cardiac microtissue maturation: A heart‐on‐chip platform embedding peristaltic pump capabilities | Abstract Heart‐on‐chip is an unprecedented technology for recapitulating key biochemical and biophysical cues in cardiac pathophysiology. Several designs have been proposed to improve its ability to mimic the native tissue and establish it as a reliable research platform. However, despite mimicking one of most vascular... | 1 INTRODUCTION Miniaturized in vitro models offer unprecedent opportunities to unravel the rules that govern biological organization (Visone et al. , 2016 ). Specifically, heart‐on‐chip technology has gained attention overtime due to its potential to guide tissue development by providing biochemical and biophysical sig... |
10. 1002/bit. 27852 | 2,021 | Biotechnology and Bioengineering | Travelling ultrasound promotes vasculogenesis of three‐dimensional‐monocultured human umbilical vein endothelial cells | Abstract To generate three‐dimensional tissue in vitro, promoting vasculogenesis in cell aggregates is an important factor. Here, we found that ultrasound promoted vasculogenesis of human umbilical vein endothelial cells (HUVECs). Promotion of HUVEC network formation and lumen formation were observed using our method. ... | 1 INTRODUCTION Tissue engineering generates tissues and organs for regenerative medicine, drug testing, and biomedical research (Esch et al. , 2015 ; Imashiro & Shimizu, 2021 ). Currently, many studies of engineering three‐dimensional (3D) tissue in vitro have been reported. However, a lack of intrinsic vascularity and... |
10. 1002/bit. 28075 | 2,022 | Biotechnology and Bioengineering | Bioprinting small‐diameter vascular vessel with endothelium and smooth muscle by the approach of two‐step crosslinking process | Abstract Three‐dimensional bioprinting shows great potential for autologous vascular grafts due to its simplicity, accuracy, and flexibility. The 6‐mm‐diameter vascular grafts are used in clinic. However, producing small‐diameter vascular grafts are still an enormous challenge. Normally, sacrificial hydrogels are used ... | 1 INTRODUCTION Cardiovascular diseases (CVDs) are considered the major cause of death world widely (Zoghbi et al. , 2014 ). The main treatment methods include medical therapy, endovascular intervention, and surgical transplantation (Buchanan et al. , 2014 ). Autologous blood vascular transplantation remains to be the g... |
10. 1002/bit. 28100 | 2,022 | Biotechnology and Bioengineering | Design, fabrication, and characterization of a multimodal reconfigurable bioreactor for bone tissue engineering | Abstract In the past decades, bone tissue engineering developed and exploited many typologies of bioreactors, which, besides providing proper culture conditions, aimed at integrating those bio‐physical stimulations that cells experience in vivo, to promote osteogenic differentiation. Nevertheless, the highly challengin... | 1 INTRODUCTION Critical size skeletal defects resulting from trauma, tumor, or pathological disorders typically require the use of bone grafts, both of human and animal origin. However, bone grafting procedures are restricted by the limited availability of bone that can be harvested from the iliac crest, and by the exp... |
10. 1002/bit. 28105 | 2,022 | Biotechnology and Bioengineering | A combined experimental and computational framework to evaluate the behavior of therapeutic cells for peripheral nerve regeneration | Abstract Recent studies have explored the potential of tissue‐mimetic scaffolds in encouraging nerve regeneration. One of the major determinants of the regenerative success of cellular nerve repair constructs (NRCs) is the local microenvironment, particularly native low oxygen conditions which can affect implanted cell... | 1 INTRODUCTION Peripheral nerve injuries (PNIs) are associated with high socioeconomic and personal costs; the mean patient age is ~30 years so they can greatly impact lifetime health and productivity (Lad et al. , 2010 ). Although nerves exhibit some regenerative capacity, the degree of reinnervation and subsequent re... |
10. 1002/bit. 28133 | 2,022 | Biotechnology and Bioengineering | Validation and scalability of homemade polycaprolactone macrobeads grafted with thermo‐responsive poly( | Abstract In this study, polycaprolactone (PCL) macrobeads were prepared by an oil‐in‐water (o/w) emulsion solvent evaporation method with poly(vinyl alcohol) (PVA) as an emulsifier and conjugated to poly( N ‐isopropylacrylamide) (PNIPAAm) to be used as cell carriers with noninvasive cell detachment properties (thermo‐r... | 1 INTRODUCTION Thermo‐responsive scaffolds containing poly( N ‐isopropylacrylamide) (PNIPAAm) are of great interest, thanks to their specific ability to release cells without physical damage upon temperature changes (Dhamecha et al. , 2021 ; Haq et al. , 2017 ; Kim et al. , 2003 ). This technique has been shown not to ... |
10. 1002/bit. 28174 | 2,022 | Biotechnology and Bioengineering | hMSCs in contact with DMSO for cryopreservation: Experiments and modeling of osmotic injury and cytotoxic effect | Abstract In this study a combined analysis of osmotic injury and cytotoxic effect useful for the optimization of the cryopreservation process of a cell suspension is carried out. The case of human Mesenchymal Stem Cells (hMSCs) from Umbilical Cord Blood (UCB) in contact with dimethyl sulfoxide (DMSO) acting as Cryo‐Pro... | 1 INTRODUCTION In tissue engineering, the preservation of biological material is a core technology to bring cell‐based products to market on‐demand (Cincotti & Fadda, 2012 ; Karlsson & Toner, 2000 ). The principal preservation method consists of freezing the bio‐specimens to cryogenic temperatures to take advantage of ... |
10. 1002/bit. 28182 | 2,022 | Biotechnology and Bioengineering | Technological advances in ocular trabecular meshwork in vitro models for glaucoma research | Abstract Glaucoma is the leading cause of irreversible blindness worldwide and is characterized by the progressive degeneration of the optic nerve. Intraocular pressure (IOP), which is considered to be the main risk factor for glaucoma development, builds up in response to the resistance (resistance to what?) provided ... | 1 INTRODUCTION Glaucoma is the leading cause of irreversible blindness worldwide. It afflicts well over 70 million people and it is estimated that this number will rise to 111. 8 million in 2040 (Tham et al. , 2014 ). Given that the first stage of the illness is asymptomatic, the number of affected individuals may actu... |
10. 1002/bit. 28201 | 2,022 | Biotechnology and Bioengineering | Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues | Abstract Bioengineered bone designed to heal large defects requires concomitant development of osseous and vascular tissue to ensure engraftment and survival. Adult human mesenchymal stromal cells (MSC) are promising in this application because they have demonstrated both osteogenic and vasculogenic potential. This stu... | 1 INTRODUCTION Cell‐based tissue engineering is being developed as an approach to the repair and regeneration of large and recalcitrant bone defects. (Forrestal et al. , 2017 ) This strategy avoids key drawbacks associated with current autologous tissue grafting, such as donor‐site morbidity, infection, and postoperati... |
10. 1002/bit. 28291 | 2,022 | Biotechnology and bioengineering | Carboxymethyl cellulose-alginate interpenetrating hydroxy ethyl methacrylate crosslinked polyvinyl alcohol reinforced hybrid hydrogel templates with improved biological performance for cardiac tissue engineering | Cardiac tissue engineering is an emerging approach for cardiac regeneration utilizing the inherent healing responses elicited by the surviving heart using biomaterial templates. In this study, we aimed to develop hydrogel scaffolds for cardiac tissue regeneration following myocardial infarction (MI). Two superabsorbent... | No full text available |
10. 1002/bit. 28316 | 2,023 | Biotechnology and bioengineering | Prosthetic Vascular Grafts Engineered to Combat Calcification: Progress and Future Directions | Calcification in prosthetic vascular conduits is a major challenge in cardiac and vascular surgery that compromises the long-term performance of these devices. Significant research efforts have been made to understand the etiology of calcification in the cardiovascular system and to combat calcification in various card... | No full text available |
10. 1002/bit. 28371 | 2,023 | Biotechnology and bioengineering | Fibrin gel enhanced trilayer structure in cell-cultured constructs | Efficient cell seeding and subsequent support from a substrate ensure optimal cell growth and neotissue development during tissue engineering, including heart valve tissue engineering. Fibrin gel as a cell carrier may provide high cell seeding efficiency and adhesion property, improved cellular interaction, and structu... | No full text available |
10. 1002/bjs. 10887 | 2,018 | The British Journal of Surgery | Meta‐analysis of the oncological safety of autologous fat transfer after breast cancer | Lipofilling ok | Introduction Breast cancer is the most common malignancy in women worldwide, with 1·7 million new cases annually and a global burden that surpasses that of all other cancers 1. Through improved early detection and treatment, the number of women surviving is gradually increasing, thereby shifting the focus towards impro... |
10. 1002/bjs5. 50220 | 2,019 | BJS Open | Effect of oxygen‐producing suture material on hypoxic colonic anastomoses in an experimental model | Background Anastomotic leak remains a significant cause of morbidity and mortality after colorectal surgery. Among multiple risk factors considered, hypoxia–ischaemia is considered to be a primary cause of intestinal anastomotic leakage. The aim of this experimental study was to assess safety, usability for surgical ta... | Introduction Despite much investigational effort, anastomotic leakage after colorectal surgery remains a significant problem associated with considerable morbidity and mortality 1, 2, 3. Hypoxia–ischaemia has been identified as one of the primary reasons for anastomotic breakdown in both experimental 4, 5 and clinical ... |
10. 1002/brb3. 1027 | 2,018 | Brain and Behavior | Epineural adipose‐derived stem cell injection in a sciatic rodent model | Abstract Background The aim was to evaluate the regenerative effect of epineural injection of rat ASC s ( rASC s) in three different settings of acute and chronic compression in a rat sciatic nerve model. Methods Acute compression (60 s) with a vessel clamp over a distance of 1 mm (group 1) or 10 mm (group 2), as well ... | 1 INTRODUCTION The outcome of peripheral nerve crush injury is often unsatisfying despite the intrinsic capability of peripheral nerve regeneration and a considerable progress in medical science over the past decades (Wood, Kemp, Weber, Borschel, & Gordon, 2011 ). The intrinsic regenerative process is with a maximum re... |
10. 1002/brb3. 2788 | 2,022 | Brain and Behavior | MiR‐30b‐5p attenuates the inflammatory response and facilitates the functional recovery of spinal cord injury by targeting the NEFL/mTOR pathway | Abstract Background Neurofilament light chain (NEFL) has been identified as a biomarker for spinal cord injury (SCI), but its effect and underlying mechanism in SCI remain unclear. Methods SCI rat models were established for in vivo studies. Lipopolysaccharide (LPS)‐induced cell models were used for in vitro studies. T... | 1 INTRODUCTION Spinal cord injury (SCI) is a devastating neurological condition usually caused by external mechanical damage (Alizadeh et al. , 2019 ). Primary and secondary injuries are the two sequential processes of SCI. Patients diagnosed with SCI have poor functional recovery and a high disability rate. During the... |
10. 1002/brb3. 813 | 2,017 | Brain and Behavior | Reflex‐based grasping, skilled forelimb reaching, and electrodiagnostic evaluation for comprehensive analysis of functional recovery—The 7‐mm rat median nerve gap repair model revisited | Abstract Introduction The rat median nerve injury and repair model gets increasingly important for research on novel bioartificial nerve grafts. It allows follow‐up evaluation of the recovery of the forepaw functional ability with several sensitive techniques. The reflex‐based grasping test, the skilled forelimb reachi... | 1 INTRODUCTION Peripheral nerve injuries (PNIs) are a major cause of morbidity, especially as in most cases the upper limbs are affected (Deumens et al. , 2010 ; Kouyoumdjian, 2006 ). In 10. 2% of all hand injuries, a peripheral nerve reconstruction is required, whereas in two third of the cases, the digital nerves are... |
10. 1002/btm2. 10009 | 2,016 | Bioengineering & Translational Medicine | Letter from the Editor | No abstract available | Dear Colleagues, I am pleased to announce the launch of Bioengineering & Translational Medicine, a new journal from AIChE and SBE, and our publishing partner, Wiley. This new journal will focus on ways Chemical and Biological Engineering drive innovations and solutions that impact clinical practice and commercial healt... |
10. 1002/btm2. 10013 | 2,016 | Bioengineering & Translational Medicine | Microfluidics‐based 3D cell culture models: Utility in novel drug discovery and delivery research | Abstract The implementation of microfluidic devices within life sciences has furthered the possibilities of both academic and industrial applications such as rapid genome sequencing, predictive drug studies, and single cell manipulation. In contrast to the preferred two‐dimensional cell‐based screening, three‐dimension... | 1 Introduction Cell culture, an alternative to organ culture and in vivo animal models, is an integral part of several ongoing studies pertaining to biomedical research including biochemistry, biology, pharmacokinetics, and pharmacodynamic discovery and development of therapeutic drugs, as well as tissue engineering. 1... |
10. 1002/btm2. 10021 | 2,016 | Bioengineering & Translational Medicine | Synthetic bioreducible lipid‐based nanoparticles for miRNA delivery to mesenchymal stem cells to induce neuronal differentiation | Abstract MicroRNA (miRNA) functions in tissue regeneration and determines the fate of stem cells. Nanoparticle‐based miRNA delivery systems for therapeutic applications have been studied in clinical settings. However, gene delivery to stem cells is still a challenging issue. Lipid‐like nanoparticles produced using comb... | 1 Introduction MicroRNA (miRNA) modulates gene expression, functions in tissue regeneration, and determines the fate of stem cells. 1, 2 Several miRNAs, such as miR‐9 and miR‐124, have been identified to play a role in neuronal differentiation of stem cells. 1, 3, 4, 5, 6 Jing et al reported that mesenchymal stem cells... |
10. 1002/btm2. 10031 | 2,016 | Bioengineering & Translational Medicine | Engineering responsive supramolecular biomaterials: Toward smart therapeutics | Abstract Engineering materials using supramolecular principles enables generalizable and modular platforms that have tunable chemical, mechanical, and biological properties. Applying this bottom‐up, molecular engineering‐based approach to therapeutic design affords unmatched control of emergent properties and functiona... | 1 Introduction The dynamic physiological and biological features presented by disease inspire the development of creative therapeutic solutions with the ability to sense indicators of pathology and respond in a way that is in itself dynamic. As such, therapeutics designed using supramolecular principles could have broa... |
10. 1002/btm2. 10034 | 2,016 | Bioengineering & Translational Medicine | Nanoengineered thermoresponsive magnetic hydrogels for biomedical applications | Abstract “Smart” hydrogels are part of an emerging class of biomaterials that respond to multiple external stimuli. A range of thermoresponsive magnetic hydrogels is currently being developed for on‐demand delivery of biomolecules for a range of biomedical applications, including therapeutic drug delivery, bioimaging, ... | 1 Introduction Stimuli‐responsive hydrogels can be designed to respond to changes in external stimuli, such as temperature, pH, light, and ultrasonic frequency. 1, 2, 3, 4, 5 Hydrogels that experience physio‐chemical changes due to a change in an external stimulus have great potential in the field of noninvasive and re... |
10. 1002/btm2. 10035 | 2,016 | Bioengineering & Translational Medicine | Evaluating side effects of nanoparticle‐mediated siRNA delivery to mesenchymal stem cells using next generation sequencing and enrichment analysis | Abstract RNA interference has immense potential to modulate cell functions. However, effective delivery of small interfering RNA (siRNA) while avoiding deleterious side effects has proven challenging. This study investigates both intended and unintended effects of diblock copolymer nanoparticle (NP) delivery of siRNA d... | 1 Introduction RNA interference (RNAi) is post‐transcriptional gene silencing resulting from homologous base pair interaction of double stranded RNA (dsRNA) molecules and target messenger RNA (mRNA). Multiple types of dsRNA, such as microRNA (miRNA) and small interfering RNA (siRNA), can initiate the RNAi pathway, resu... |
10. 1002/btm2. 10040 | 2,016 | Bioengineering & Translational Medicine | Introduction to Editorial Board Members: Professor David A. Tirrell | No abstract available | In this second issue of Bioengineering and Translational Medicine, we are pleased to introduce our Editorial Board Member, Prof. David A. Tirrell. Prof. Tirrell is the Ross McCollum‐William H. Corcoran Professor of Chemistry and Chemical Engineering and Director of the Beckman Institute at California Institute of Techn... |
10. 1002/btm2. 10041 | 2,016 | Bioengineering & Translational Medicine | Peptide‐based supramolecular hydrogels for delivery of biologics | Abstract The demand for therapeutic biologics has rapidly grown over recent decades, creating a dramatic shift in the pharmaceutical industry from small molecule drugs to biological macromolecular therapeutics. As a result of their large size and innate instability, the systemic, topical, and local delivery of biologic... | 1 Introduction Biologics, including monoclonal antibodies (mAbs), cytokines, growth factors, enzymes, vaccines, and genes, are biomedical products that are produced or extracted from a living system such as human, animal, or microorganism. In most cases, biologics refer to the biopharmaceuticals produced through recomb... |
10. 1002/btm2. 10045 | 2,016 | Bioengineering & Translational Medicine | Introduction to special issue on “Responsive Materials and Systems: Toward Smart and Precision Medications” | No abstract available | Spurred by advances in materials chemistry, molecular pharmaceutics and micro/nanobiotechnology, stimuli‐responsive “smart” materials and systems have been studied extensively for various applications, including drug delivery, diagnosis, tissue engineering, and biomedical devices. In drug delivery, the dosage‐, spatial... |
10. 1002/btm2. 10060 | 2,017 | Bioengineering & Translational Medicine | Reduced arterial elasticity due to surgical skeletonization is ameliorated by abluminal PEG hydrogel | Abstract Arteries for bypass grafting are harvested either with neighboring tissue attached or as skeletonized vessels that are free of surrounding tissue. There are significant benefits to skeletonization, but reports suggest that skeletonized vessels may develop structural defects and are at risk for atherosclerosis.... | 1 INTRODUCTION Surgical grafting and reconstruction are principal treatments for coronary and peripheral artery diseases 1 and for the repair of congenital cardiovascular malformations. 2 Two established harvesting techniques, pedicled and skeletonized, are used for arterial grafts. In the preparation of pedicled graft... |
10. 1002/btm2. 10062 | 2,017 | Bioengineering & Translational Medicine | Human pluripotent stem cell‐derived epicardial progenitors can differentiate to endocardial‐like endothelial cells | Abstract During heart development, epicardial progenitors contribute various cardiac lineages including smooth muscle cells, cardiac fibroblasts, and endothelial cells. However, their specific contribution to the human endothelium has not yet been resolved, at least in part due to the inability to expand and maintain h... | 1 INTRODUCTION The epicardium is the outermost mesothelium layer of the heart that is essential for both heart development and cardiac remodeling. 1 During cardiogenesis, a subset of epicardial cells invades the underlying myocardium and contributes to various cardiac lineages, including cardiac fibroblasts and smooth ... |
10. 1002/btm2. 10063 | 2,017 | Bioengineering & Translational Medicine | Designing biomaterials with immunomodulatory properties for tissue engineering and regenerative medicine | Abstract Recent research in the vaccine and immunotherapy fields has revealed that biomaterials have the ability to activate immune pathways, even in the absence of other immune‐stimulating signals. Intriguingly, new studies reveal these responses are influenced by the physicochemical properties of the material. Nearly... | 1 INTRODUCTION Biomaterials have enabled advances in fields spanning tissue engineering, drug delivery, vaccination and immunotherapies, and implantable devices. This breadth is due to the ability of these materials to encapsulate and protect cargos (e. g. , chemicals, cells, and proteins), to provide biocompatible sup... |
10. 1002/btm2. 10071 | 2,017 | Bioengineering & Translational Medicine | Introduction to Special Issue: 2016 Translational Medicine and Bioengineering Conference | No abstract available | Bioengineering & Translational Medicine was launched in 2016 with the goal to highlight the scientific and technological issues relevant to the translational challenges of bioengineering science and technologies. Clinical translation of promising biological technologies can have a transformative impact on human health.... |
10. 1002/btm2. 10076 | 2,017 | Bioengineering & Translational Medicine | The Wyss institute: A new model for medical technology innovation and translation across the academic‐industrial interface | Abstract The Wyss Institute for Biologically Inspired Engineering at Harvard University was formed based on the recognition that breakthrough discoveries cannot change the world if they never leave the laboratory. The Institute's mission is to discover the biological principles that Nature uses to build living things, ... | 1 INTRODUCTION Engineering has transformed medicine over the past 50 years by applying engineering principles to solve medical problems. This has led to major medical breakthroughs, including heart bypass and dialysis machines, respiratory ventilators, pacemakers, endoscopes, stents, orthopedic implants, drug delivery ... |
10. 1002/btm2. 10077 | 2,018 | Bioengineering & Translational Medicine | Visible light crosslinkable human hair keratin hydrogels | Abstract Keratins extracted from human hair have emerged as a promising biomaterial for various biomedical applications, partly due to their wide availability, low cost, minimal immune response, and the potential to engineer autologous tissue constructs. However, the fabrication of keratin‐based scaffolds typically rel... | 1 INTRODUCTION Protein‐based biomaterials, such as those based on silk fibroin, 1, 2 collagen, 3, 4 and elastin, 5 have been extensively studied to fabricate tissue engineered scaffolds. In general, protein‐based biomaterials demonstrate excellent biocompatibility and biodegradability via enzymatic or hydrolytic pathwa... |
10. 1002/btm2. 10078 | 2,017 | Bioengineering & Translational Medicine | Soluble Collagen VI treatment enhances mesenchymal stem cells expansion for engineering cartilage | Abstract Bone Marrow‐derived mesenchymal stem cells (BM‐MSC) are an attractive source for cell‐based therapies in cartilage injury owing to their efficient differentiation into chondrocytes and their immune‐suppressive abilities. However, their clinical use is hampered by a scarcity of cells leading to compromised effi... | 1 INTRODUCTION Osteoarthritis (OA) is a multifactorial disease that affects the articular cartilage causing deterioration of the joint function. Cartilage injuries and trauma are difficult to repair even in young adults due to the poor regenerative potential of the cartilage tissue, and greatly accelerates OA developme... |
10. 1002/btm2. 10087 | 2,018 | Bioengineering & Translational Medicine | BioTM Buzz (Volume 3, Issue 1) | No abstract available | Volume 3, Issue 1 Rapid‐forming keratin‐based hydrogels Protein‐based biomaterials are widely utilized as scaffolds for tissue engineering applications given their advantages related to biocompatibility and controllable degradation via enzymes. However, issues related to synthesis and manufacturing have limited their c... |
10. 1002/btm2. 10104 | 2,018 | Bioengineering & Translational Medicine | Rescuing mesenchymal stem cell regenerative properties on hydrogel substrates post serial expansion | Abstract The use of human mesenchymal stem/stromal cells (hMSCs) in most clinical trials requires millions of cells/kg, necessitating ex vivo expansion typically on stiff substrates (tissue culture polystyrene [TCPS]), which induces osteogenesis and replicative senescence. Here, we quantified how serial expansion on TC... | 1 INTRODUCTION Human mesenchymal stem/stromal cells (hMSCs) are multipotent cells capable of differentiating into cell types found in tissues of the mesoderm (bone, cartilage, and fat), ectoderm (epithelium and neural), and endoderm (muscle, gut, and lung). 1 hMSCs are characterized by a cell surface maker profile, whi... |
10. 1002/btm2. 10110 | 2,018 | Bioengineering & Translational Medicine | Applications of decellularized extracellular matrix in bone and cartilage tissue engineering | Abstract Regenerative therapies for bone and cartilage injuries are currently unable to replicate the complex microenvironment of native tissue. There are many tissue engineering approaches attempting to address this issue through the use of synthetic materials. Although synthetic materials can be modified to simulate ... | 1 INTRODUCTION Regenerative medicine offers the ability to repair injuries that the body fails to heal. Although there are many synthetically designed materials to support tissue regeneration, these materials fall short of fully replicating a tissue's microenvironment. 1 Looking to the function of this microenvironment... |
10. 1002/btm2. 10111 | 2,018 | Bioengineering & Translational Medicine | Encapsulation of mesenchymal stem cells in glycosaminoglycans‐chitosan polyelectrolyte microcapsules using electrospraying technique: Investigating capsule morphology and cell viability | Abstract Polyelectrolyte microcapsules are modular constructs which facilitate cell handling and assembly of cell‐based tissue constructs. In this study, an electrospray (ES) encapsulation apparatus was developed for the encapsulation of mesenchymal stem cells (MSCs). Ionic complexation between glycosaminoglycans (GAGs... | 1 INTRODUCTION Progress in the biofabrication of implantable, engineered tissue is slowed by the challenge of assembling three‐dimensional tissue with a fully integrated microvasculature. One strategy for tissue and vessel assembly involves the fusion‐based assembly of endothelialized cell spheroids. This approach hold... |
10. 1002/btm2. 10114 | 2,018 | Bioengineering & Translational Medicine | Engineering molecular imaging strategies for regenerative medicine | Abstract The reshaping of the world's aging population has created an urgent need for therapies for chronic diseases. Regenerative medicine offers a ray of hope, and its complex solutions include material, cellular, or tissue systems. We review basics of regenerative medicine/stem cells and describe how the field of mo... | Abbreviations ADME absorption distribution metabolism excretion ASC adult stem cell AuNP gold (Au) nanoparticle BLI bioluminescence imaging BRET bioluminescence resonance energy transfer CAG chicken beta‐actin/rabbit beta globin hybrid promoter CAR‐T chimeric antigen receptor T cell CCD charged coupled device CMV cytom... |
10. 1002/btm2. 10116 | 2,018 | Bioengineering & Translational Medicine | BioTM Buzz (Volume 3, Issue 3) | No abstract available | BioTM Buzz Volume 3, Issue 3 Bugs secreting drugs Recent interest in microbe based therapeutics have led to the development of novel approaches to deliver drugs for treatment of enteric infections. In this issue of Bioengineering & Translational Medicine, a team of researchers at General Probiotics in Minnesota describ... |
10. 1002/btm2. 10117 | 2,018 | Bioengineering & Translational Medicine | Introduction to Editorial Board Member: Professor Kristi S. Anseth | No abstract available | 1 In this issue of Bioengineering and Translational Medicine, we are pleased to introduce our Editorial Board Member, Prof. Kristi S. Anseth. Prof. Anseth is a Distinguished Professor and the Tony Tisone Endowed Chair in Chemical and Biological Engineering, Associate Professor of Surgery, and the Associate Director of ... |
10. 1002/btm2. 10124 | 2,018 | Bioengineering & Translational Medicine | Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics | Abstract Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allo... | 1 INTRODUCTION Biomaterials play a pivotal role in designing functional scaffolds, providing three‐dimensional (3D) templates that facilitate cell adhesion, growth, proliferation, and differentiation. Engineered scaffolds may promote vascularization and tissue formation, which are essential for tissue engineering and r... |
10. 1002/btm2. 10125 | 2,018 | Bioengineering & Translational Medicine | BioTM Buzz (Volume 4, Issue 1) | No abstract available | SPECIAL ISSUE: TRIBUTE TO ROBERT LANGER AND NICHOLAS PEPPAS In this Special Issue of Bioengineering & Translational Medicine, we honor two luminaries in the AIChE community who pioneered the field of drug delivery and biomaterials, Professors Robert Langer, and Nicholas Peppas. These two giants, born within 4 days of e... |
10. 1002/btm2. 10138 | 2,019 | Bioengineering & Translational Medicine | Leader cell PLCγ1 activation during keratinocyte collective migration is induced by EGFR localization and clustering | Abstract Re‐epithelialization is a critical step in wound healing and results from the collective migration of keratinocytes. Previous work demonstrated that immobilized, but not soluble, epidermal growth factor (EGF) resulted in leader cell‐specific activation of phospholipase C gamma 1 (PLCγ1) in HaCaT keratinocytes,... | Abbreviations ECM extracellular matrix EGF epidermal growth factor FBS fetal bovine serum PBS phosphate buffered saline PLCγ1 phospholipase C gamma 1 TGFβ transforming growth factor β 1 INTRODUCTION Collective migration results from the coordinated movement of cells that are in physical contact. This form of migration ... |
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