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41901096
Curcumin-Loaded Lactoferrin/Pectin Core-Shell Structured Microgel Nanoparticles: Dual Regulatory Effects in Alleviating Inflammatory Bowel Disease.
<h4>Background</h4>Curcumin (Cur) has therapeutic potential for inflammatory bowel disease (IBD) but is limited by its poor bioavailability.<h4>Methods</h4>This study demonstrated that Cur-loaded core-shell structured microgel nanoparticles (LF/CP-Cur MN), fabricated through electrostatic complexation between lactoferr...
41900076
Tetrahydrocarbazole as a Versatile Scaffold in Drug Discovery: A Cross-Target SAR Analysis and Design Paradigms.
Tetrahydrocarbazole (THCz) is a privileged scaffold validated by clinically approved drugs such as ondansetron, frovatriptan, and ramatroban and exhibits diverse bioactivities including antimicrobial, antitumor, antidiabetic, and neuroprotective effects. Despite extensive structure-activity relationship (SAR) studies, ...
41900075
Effect of Water Vapor Generated by Fresh-Cut Mango (&lt;i&gt;Mangifera indica&lt;/i&gt;) on the Release of β-Carotene from β-Cyclodextrin Inclusion Complexes Under Modified-Atmosphere Packaging.
This study evaluated the effect of water vapor generated by fresh-cut mango (<i>Mangifera indica</i>) on the release of β-carotene from β-cyclodextrin complexes (β-C:β-CD) under stored Modified Atmosphere Packaging (MAP) and to demonstrate β-carotene stabilization and passive-active packaging behavior under MAP conditi...
41898525
The Art of Domesticating Proteins: How Cancer Cells Adapt to Therapeutic and Environmental Stressors.
Cellular survival and adaptability depend on the dynamic regulation of proteins-the central actors of biological systems. Through mechanisms such as post-translational modifications, protein turnover, and the formation of membraneless organelles, cells can sense and respond to a variety of stressors. Recent advances in...
41898312
Innovative Inclusion Complexes Clotrimazole: Hydroxypropyl-β-Cyclodextrin-Modified Polyurethane Networks as Carriers for Slow Drug Delivery.
<b>Background/Objectives</b>: Inclusion complexes among drugs and cyclodextrin-modified polymers are a topic of recent interest in pharmaceutical research and industry as they might expand the solubility, bioavailability, and stability of the guest molecules. Polyurethanes derived from cyclodextrins show some biomedica...
41897891
A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus, Astrovirus, and Rotavirus.
To develop a rapid detection tool for major bovine diarrheal viruses, Bovine viral diarrhea virus (BVDV), Bovine astrovirus (BAstV), and Bovine rotavirus (BRV), a gold nanoparticle-enhanced dual-priming oligonucleotide (DPO) multiplex PCR method was established. After optimization, the assay showed high sensitivity wit...
41897744
Germination-Driven Modulation of Phenolic Compounds in Sorghum: Bioactivity and Gene Expression.
Controlled germination has emerged as an effective and sustainable bioprocess to enhance the nutritional and functional quality of cereal grains, including sorghum, a climate-resilient crop widely cultivated in tropical and semi-arid regions. Germination triggers coordinated physiological and molecular responses that a...
41897742
New Insight into the Multi-Scale Structure and Anti-Digestibility of Nano-Scale Amylopectin Ternary Assemblies Prepared Under High-Power Ultrasound.
High-power ultrasound has been widely used to regulate the anti-digestibility of starch-based products, including the formation of resistant starch (RS-V) in amylopectin assemblies. This can contribute to the attenuation of postprandial hyperglycemia. However, the mechanisms by which high-power ultrasound modulates RS-...
41890954
Research progress on self-assembled supramolecular bioactive materials from herbal natural products and their applications in anti-infection field.
Infections have always been a major factor threatening human health. The development of anti-infective drugs has made outstanding contributions to curbing the spread of infectious diseases since the 20th century. However, the efficacy of traditional anti-infective drugs is increasingly limited with the emergence of mul...
41856078
Dynamic regulation of TASK-1 channels under cellular stress.
TASK-1 channel is important for regulating membrane potential. However, its response to pathological conditions remains unknown. In this study, we investigated the effects of hypoxia on the recombinant TASK-1 channel localization at the surface of HEK293T cells. The hypoxic condition was induced via sodium cyanide (NaC...
41855792
Host-guest structure and tunable multicolor emission in Zn-MOFs via dye encapsulation.
Metal-organic frameworks (MOFs) offer significant potential for tunable luminescence due to their structural versatility. This study investigated the MOFs (Bio-MOF-1-Me) with an anionic structure, which exhibited extremely strong intrinsic ultraviolet fluorescence. To achieve color tunability, Bio-MOF-1-Me undergoes io...
41850021
Molecular bridge-guided conformal carbon coating on SiO for mechanically robust and electrochemically stable anodes.
The development of high-performance anode materials is essential for advancing lithium-ion batteries (LIBs) with higher energy density and faster charging capability. Silicon monoxide (SiO) has attracted considerable attention because of its high theoretical capacity. However, its practical application is hindered by s...
41849906
Python-assisted bimetallic-nanozyme fluorescence capillary imprinted sensor for rapid visual detection of nonylphenol ethoxylates and nonylphenol.
Nonylphenol polyethoxylate (NPEO) and its primary metabolite nonylphenol (NP) are novel endocrine disrupting compounds and emerging pollutants, resulting in hazards toward human health. Developing a portable, ultrasensitive and rapid detection method toward NPEO and NP is a great significance for protecting human healt...
41849902
A fluorescein modified fluorescence sensor for simultaneous discrimination and detection of Fe&lt;sup&gt;3+&lt;/sup&gt; and nitrofurazone.
Fe<sup>3+</sup> and nitrofurazone (NFZ) can raise a risk for environment and human health due to accumulation in water. Hence, a post-synthetic modification strategy was employed to develop a visualization sensor for detecting Fe<sup>3+</sup> and NFZ. In this study, we selected a Zinc-based metal organic framework in c...
41849892
Recent trends in MOF-based electrochemical biosensing strategies for cardiovascular disease diagnostics.
Cardiovascular diseases (CVDs) remain a leading cause of death worldwide, creating an urgent need for diagnostic technologies that are rapid, accurate, and affordable. Conventional diagnostic methods, such as electrocardiograms and chest X-ray imaging, are often limited by high costs, time-intensive procedures, and rel...
41839472
Host-guest confinement in post-metallized COFs for enhanced electrochemiluminescence coupled with Y-shaped DNA amplification for microRNA-499 detection.
An ultrasensitive electrochemiluminescence (ECL) biosensor was developed for the detection of the acute myocardial infarction (AMI) biomarker miRNA-499 by synergistically integrating an iridium-metallized covalent organic framework (Ir@TpBpy) with enzyme-assisted Y-shaped DNA amplification. The Ir@TpBpy COF was synthes...
41839327
The label-free electrochemical S100B-TBI immunosensor for sensitive detection of S100B protein as a biomarker for traumatic brain injury using FFT-SWV voltammetry.
S100B protein is an established biomarker related to prognosis in traumatic brain injury (TBI). Developing sensitive methods for the serum S100B quantification is clinically valuable. This paper proposes a simple label-free electrochemical TBI-immunosensor for the timely detection of S100B in the sera of TBI patients. ...
41839266
In situ delivery of JPH203 via camptothecin-peptide conjugate nanoassemblies to trigger ferroptosis in triple-negative breast cancer.
Triple-negative breast cancer (TNBC) remains a formidable clinical challenge due to the lack of druggable targets and intrinsic resistance to conventional therapies. Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation, represents a promising therapeutic vulnerability in TNBC. Here, ...
41839263
ROS-responsive self-assembly of baicalin-peptide as a "Molecular Latch" to break the endothelial-to-mesenchymal transition-inflammation loop in atherosclerotic plaques.
Atherosclerotic plaque rupture, driven by a vicious pathological cycle between endothelial-to-mesenchymal transition (EndMT) and chronic inflammation, represents a major therapeutic challenge in cardiovascular disease. Current clinical strategies, including statins and antiplatelet agents, fail to disrupt the EndMT-inf...
41839234
Process for standardizing and assessing the parameters governing MS2 virus-like particle reassembly around nucleic acid cargo.
MS2 virus-like particles (VLPs) are widely used as protein nanocages for cargo encapsulation, yet in vitro disassembly-reassembly protocols remain poorly standardized, and reassembly yields are reported inconsistently. As a result, the same experiments reported in literature produce widely divergent yields, limiting re...
41833836
Discrete 3' UTR sequence motifs regulate nanos RNA enrichment in Drosophila germ granules.
Germ granules are ribonucleoprotein condensates that concentrate key maternal mRNAs needed for germ cell development. In Drosophila, nanos mRNA is selectively enriched in germ granules but the specific cis-acting elements mediating this process remain poorly defined. Here, we identify discrete sequence motifs in the na...
41832487
CD93-targeted resveratrol-loaded PLGA nanoparticles remodel CD8⁺ T cell metabolism through AIF-mediated oxidative phosphorylation to overcome lung cancer immunotherapy resistance.
Lung cancer (LC) remains a leading cause of cancer-related mortality worldwide, and the limited efficacy of immunotherapy due to treatment resistance underscores the urgent need for new therapeutic strategies. In the present study, CD93-targeted poly(lactic-co-glycolic acid) (PLGA) nanoparticles encapsulating resveratr...
41832189
Turn-on luminescence from molecular rotor realignment in metal-organic framework thin films.
Sub-unit motion within metal-organic frameworks (MOFs) offers unique opportunities for nanoscale sensing. However, achieving controlled partial rotation of bulky linkers remains a significant challenge. In this study, a 50-fold luminescence enhancement is observed from a MOF thin film when intra-pore solvent flow orien...
41831077
Biomimetic mineralization of chemically modified glucose oxidase from Aspergillus niger in ZIF-8.
Chemical surface modification of enzymes represents a powerful strategy to improve immobilization and catalytic performance. In this study, glucose oxidase (GOx) from Aspergillus niger was chemically modified via periodate oxidation and reductive amination to incorporate L-aspartate (D-GOx) and L-histidine (H-GOx) resi...
41831026
High-Affinity Three-Way Junction Aptamers for Sensing Cationic Polymyxin Antibiotics.
Polymyxins B and E (colistin) were historically the last-resort antibiotics for treating infections caused by multidrug-resistant Gram-negative bacteria. However, their narrow therapeutic windows and significant nephrotoxicity necessitate rapid and precise detection methods. In this study, we employed a mixture of poly...
41830795
Self-Assembly of (l)-Cysteine Molecules at Ag(110): A Scanning Tunneling Microscopy and X-ray Photoemission Spectroscopy Study.
Organic-inorganic interactions are at the basis of relevant applications, ranging from biocompatibility and nano(bio)technology to antifouling and hygiene issues. A fundamental understanding of the processes occurring at the interface between molecules of biological interest and inorganic surfaces is a key issue to opt...
41830632
Interfacial Metal Nanocluster Conduits Direct Charge Transfer for Record Unassisted Solar Water Splitting.
Photoelectrochemical (PEC) water splitting offers one of the most promising solutions for sustainable solar-to-chemical fuel conversion. However, sluggish charge migration across the photoelectrode interface fundamentally limits the PEC efficiency. Herein, we design and engineer an atomic-scale interfacial charge condu...
41830610
Discovery of Partner Protein-Dependent Graspetide Biosynthesis.
Graspetides represent a class of ribosomally synthesized and post-translationally modified peptide (RiPP) that can contain macrolactone/macrolactam linkages from amino acid side chains. Many predicted graspetide biosynthetic gene clusters (BGCs) contain untapped tailoring enzymes, including some with the potential to m...
41830201
Glycinamide-Driven Coumarin Construction (GDCC): A Mild Strategy for Fluorescent Labeling and Macrocyclization of Peptides.
Peptidic macrocycles have attracted increasing attention due to their well-defined structures and enhanced biological activities. However, efficient cyclization of residues lacking reactive side chains remains a significant challenge. Herein, we report a glycinamide-driven coumarin construction (GDCC) reaction that ena...
41830206
Efficient Circularly Polarized Near-Infrared Luminescence from Zero-Dimensional Hybrid Copper Bromides.
Circularly polarized luminescence (CPL) provides an additional degree of freedom for light manipulation beyond intensity and wavelength and is essential for advanced photonic technologies. Chiral hybrid metal halides (HMHs) have recently emerged as promising CPL-active materials. However, the simultaneous realization o...
41826349
Coenzyme-functionalized photo-redox catalysis for low-energy click labeling.
Photo-redox catalysis has emerged as a new generation of biochemical protocols in proximity labeling, noninvasive therapy, ultrafast activation. However, the well-developed photocatalysts cannot simultaneously possess low-energy absorption and high excited potentials to initiate photochemical reactions. Herein, we deve...
42005816
Stimuli-responsive hydrogels for radiation-induced skin injury: from passive barriers to autonomous drug delivery systems.
Radiation-induced skin injury (RISI) affects over 95% of radiotherapy patients. Current clinical management remains confined to passive supportive care, lacking mechanistic precision for RISI's unique pathophysiology. This review adopts a function-centric perspective, classifying hydrogel systems across three generatio...
41983047
Advanced small extracellular vesicles delivery systems for &lt;i&gt;in situ&lt;/i&gt; tissue engineering.
<i>In situ</i> tissue engineering, which activates the body's innate regenerative capacity, has demonstrated superior clinical translation potential than traditional <i>ex situ</i> approaches. Small extracellular vesicles (sEVs), as natural nanovesicles, can excellently mimic the paracrine functions of cells and are th...
41978640
Affinity-optimized TROP2 antibodies support potent antitumor activity in antibody-drug conjugates.
<h4>Background</h4>Trophoblast cell surface antigen 2 (TROP2) is frequently overexpressed in epithelial tumors and is associated with poor prognosis, making it an attractive therapeutic target. Antibody-drug conjugates (ADCs) directed against TROP2 show clinical benefit, but expression in normal tissues such as skin ra...
41952993
Drug screening to identify compounds to eliminate &lt;i&gt;Burkholderia pseudomallei&lt;/i&gt; through Hcp protein.
<i>Burkholderia pseudomallei</i> (<i>B. pseudomallei</i>) exhibits inherent resistance to multiple antibiotics and Strong pathogenicity, highlighting the urgent need for effective antibiotics. The type VI secretion system (T6SS) is a key virulence factor in <i>B. pseudomallei</i>, and its core structural protein hemoly...
41952987
Increased anti-SARS-CoV-2 antibody titers in plasma-derived intravenous immunoglobulin collected in Southern China.
Although the COVID-19 pandemic is no longer considered an emergency, the ongoing spread of the virus poses a persistent threat to public health. COVID-19 poses a challenge to the system due to the risk of saturation and the associated economic burden. To assess changes in the anti-SARS-CoV-2 antibody profile, we analyz...
41941317
Methods of Enhancing the Solubility and Bioavailability of BCS Classification II Drugs.
The solubility and bioavailability of drugs have a significant impact on their therapeutic efficacy, particularly for BCS (Biopharmaceutical Classification System) Class II drugs, which have low solubility and high permeability. Inconsistent bioavailability and a diminished pharmacological response result from poor sol...
41935011
CRISPR/Cas12a-mediated electrochemiluminescent biosensor integrating Ag modified Co-doped metal-organic frameworks for dual detection of malathion and phorate.
<h4>Background</h4>With growing concerns about food safety, the detection of pesticide residues in food has become increasingly important. The mixed organophosphorus pesticides (OPs) formulations are commonly employed to maximize crop production; however, the excessive application of OPs has posed severe threats to foo...
41930497
Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay.
Interleukin-26, a cytokine belonging to the IL-10 family, is an inflammatory mediator in mammals and has been shown to modulate T cell proliferation and gene expression in avian species. Given the limited understanding of chIL-26's function at the protein level, our objective was to produce specific mAbs to chIL-26 and...
41918629
Synthesis and antimicrobial activity of sulfonyl-imidazole linked fused isoxazolo[3,4-b][1,2,3]triazolo[4,5-d]pyridines:PEG-400 mediated one-pot reaction under ultrasonic irradiation.
<h4>Introduction</h4>The rapid emergence of methicillin-resistant and vancomycin-resistant <i>Staphylococcus aureus</i> (MRSA and VRSA) represents a major global health challenge, necessitating the development of new antibacterial scaffolds with improved efficacy and safety.<h4>Methods</h4>In this study, a novel series...
41909682
Wilforine attenuates inflammatory osteolysis by suppressing osteoclast fusion through JAK-STAT-stomatin immunoregulatory signaling.
<h4>Background</h4>Excessive osteoclast fusion and activation are central drivers of bone erosion in inflammatory osteopathies, which are closely linked to immune dysregulation. Wilforine, a natural compound, exhibits immunomodulatory and therapeutic potential, yet its precise mechanism of action-particularly its influ...
41909648
Exploring the host-pathogen interaction and genome analysis of multidrug-resistant bacterial pathogen &lt;i&gt;Proteus penneri&lt;/i&gt; isolated from &lt;i&gt;Labeo rohita&lt;/i&gt;.
Multidrug-resistant (MDR) bacterial pathogens represent an escalating challenge to sustainable aquaculture, particularly in high-value freshwater species such as <i>Labeo rohita</i>, a cornerstone of South Asian aquaculture. This study provides the first comprehensive integration of genomic, immunological, and microbio...
41909269
Regulation of potassium homeostasis in &lt;i&gt;Mycoplasma bovis&lt;/i&gt; by the diadenylate cyclase CdaM.
Cyclic di-AMP (c-di-AMP) is a critical second messenger in many Gram-positive bacteria and archaea that regulates intracellular potassium (K<sup>+</sup>) concentrations, ensuring osmotic balance. However, the precise mechanisms of K<sup>+</sup> regulation by c-di-AMP in <i>Mycoplasma</i> species remain largely unexplor...
41909169
Oral Poly(N-oxide) zwitterionic nanoplatform for Gambogenic Acid Enhances Mucosal penetration for potentiated anti-angiogenic therapy.
Oral anti-angiogenic therapy for hepatocellular carcinoma is fundamentally constrained by poor mucus penetration and enterocyte uptake, which limits intestinal absorption and systemic exposure. Moreover, anti-angiogenic therapy inherently reduces tumor vascular density, further restricting intratumoral drug accumulatio...
41908552
Integrated microbiome-metabolome analysis reveals multiorgan toxicity of 1-nitropyrene and the limited efficacy of ferroptosis inhibitor Fer-1 in rats.
<h4>Introduction</h4>1-Nitropyrene (1-NP), a prevalent nitro-polycyclic aromatic hydrocarbon, is increasingly recognized as a potential metabolic disruptor, yet its systemic biological effects remain insufficiently characterized.<h4>Methods</h4>This study investigated the metabolic, immunological, hepatic, and microbio...
41908481
Sustainable Encapsulation of Biocontrol Agents: Cross-Linker Influence on Carboxymethylcellulose-Based Microbeads.
The use of biopolymers for encapsulating active ingredients is a well-established approach, with ionotropic gelation representing a viable technique. This method allows the use of various cross-linking agents, though the physicochemical properties of the resulting materials can vary depending on the cross-linker select...
41908450
Investigating the Therapeutic Activity of C&lt;sub&gt;4&lt;/sub&gt;N as a Targeted Drug Delivery System for Acetaminophen and Thioguanine through DFT Simulations.
The targeted drug delivery systems (TDDS) minimize the adverse side effects of traditional drug delivery methods on healthy tissues. In the current study, the C<sub>4</sub>N nanocarrier for antipyretic and anticancer drugs acetaminophen (APAP) and thioguanine (TG), respectively, is investigated by using density functio...
41908397
Co-BDC MOF/Graphene Nanohybrid as an Efficient Electrode Material for High-Performance Supercapacitors.
A Co-BDC metal-organic framework/reduced graphene oxide (rGO) hybrid was synthesized via in-situ MOF growth followed by controlled pyrolysis to obtain a Co-BDC-derived nanoporous carbon (NPC)/rGO electrode for supercapacitors. The rGO-assisted transformation preserves the precursor integrity and yields a uniform, condu...
41908379
Carbon Cage Nanosensors for Selective Detection of Toxic Gas Molecules.
The detection of toxic substances is of utmost importance in diverse fields, from industry to medicine, and their precise discrimination could lead to significant advances in all of them. Here, we propose a novel nanosensor with a carbon cage structure that can effectively discriminate between 5 types of toxic gas mole...
41907849
Solid lipid nanoparticles as carriers for &lt;i&gt;Ulva rigida&lt;/i&gt; peptide extract with demonstrated anti-β-amyloid and antioxidant properties.
<h4>Introduction</h4>Alzheimer's disease (AD) remains one of the greatest challenges in neurodegenerative research, largely due to the toxic aggregation of the β-amyloid (Aβ) peptide and the difficulty of delivering therapeutic compounds across the blood-brain barrier (BBB).<h4>Methods</h4>This work explored <i>Ulva ri...
41907643
Aptamers targeting immune checkpoints for tumor immunotherapy: a comprehensive review.
Tumor immunotherapy has transformed the cancer treatment paradigm by leveraging the host immune system to identify and eradicate tumor cells in the body. Immune checkpoint inhibitors (ICIs) targeting programmed cell death protein 1 (PD-1), programmed death ligand 1 (PD-L1), and cytotoxic T-lymphocyte-associated antigen...
41907306
Next-generation Janus kinase inhibitors: Integrating synthetic innovation, structural biology, and computational design for precision drug discovery.
Janus kinase (JAK) dysregulation plays a central role in the pathogenesis of inflammatory, autoimmune, and malignant disorders, making the JAK family an essential therapeutic target across multiple disease domains. Over the past two decades, the field has progressed from the identification of early JAK2 inhibitors to t...
41903459
Estimation of genetic parameters for growth traits of white-feather broilers and research on genotype-environment interaction.
This study aimed to estimate the variance components of growth traits in white-feathered broilers under different environments and detect the genotype-by-environment interaction (G × E) effects of the same trait across various environments. Using growth performance records of white-feathered broilers from 6 batches und...
41902259
Advances in Nanotechnology for the Treatment of Herpes Virus Infections.
Herpes simplex virus (HSV) infections present a major global health burden due to their high morbidity. Conventional therapies offer limited efficacy due to poor bioavailability, the need for frequent administration and potential drug resistance. Recent advances in nanotechnology provide opportunities to overcome these...
41902261
Systematic Identification of the Functional lncRNAs During H7N9 Avian Influenza Virus Infection in Mice.
Accumulating studies have identified the pivotal role of long non-coding RNAs (lncRNAs) in participating in host-virus interactions during virus infections. However, the regulatory roles of lncRNAs in influenza A virus (IAV) infection are still not fully elucidated. In this study, using high-throughput sequencing, we c...
41901986
Real-Time Probing of Molecular Affinity Using Optical Tweezers.
The ability to assess molecular binding kinetics in real time is critical for advancing our understanding of molecular interactions in biochemical and biotechnological systems. This work presents a novel optical tweezer (OT)-based method to monitor molecular affinity in real time, focusing on the high-affinity streptav...
41901833
Advanced Cellulose-Based Materials: From Nanoparticles to Complex Structures and Composites.
Cellulose has long been recognised as the fundamental structural component of plant cell walls and is currently established as a highly adaptable molecular scaffold for next-generation sustainable materials [...].
41901319
Overview of Research on Essential Oils of &lt;i&gt;Zanthoxylum bungeanum&lt;/i&gt;: Composition, Activity, Applications, and Challenges.
As the main active component of <i>Zanthoxylum bungeanum</i>, its volatile oil (ZEO) exhibits diverse pharmacological activities, including insecticidal, antibacterial, anti-inflammatory, and anti-tumor effects. These properties support its traditional functions, such as "expelling worms" and "warming the Middle Jiao t...
41900847
Ultradeformable Vesicles for Wound Healing: Ethosomes, Transferosomes, and Transethosomes in Topical Drug Delivery.
Wound healing is a dynamic and multifaceted biological process involving hemostasis, inflammation, proliferation, and tissue remodeling. Topical therapy is widely preferred for wound management due to its localized action and reduced systemic adverse effects. However, the effective delivery of therapeutic agents is oft...
41900848
Tailoring the Design of Dendritic Thermogels Through Carbosilane and Polyglycerol Crosslinkers.
<b>Background/Objectives:</b> The development of stimuli-responsive hydrogels for biomedical uses is an intense field of research. The use of dendritic crosslinkers can enhance the control over the structure and properties of the networks. This work presents a comparative study on the design and evaluation of Pluronic ...
41900843
Gadolinium Nanoparticles: Emerging Platforms Beyond Imaging for Drug Delivery and Theranostics.
Gadolinium nanoparticles (GdNPs) have gained increasing attention as multifunctional metal-based nanoplatforms that extend far beyond their traditional use as magnetic resonance imaging (MRI) contrast agents. Their specific magnetic properties, tunable physicochemical features, and tunable biocompatibilities with bioco...
41900846
Advancements in Therapeutic Deep Eutectic Solvents as Multifunctional Transdermal Delivery Systems.
The human skin, specifically the stratum corneum (SC), remains a formidable barrier to the delivery of therapeutic agents. Nearly 80% of new drug candidates exhibit poor solubility or low permeability, necessitating the development of novel delivery vehicles. Therapeutic deep eutectic solvents (TheDES) have emerged as ...
41900415
Design of a Multi-Epitope Vaccine Against Ovine &lt;i&gt;Pasteurella multocida&lt;/i&gt; Using Immunoinformatics Strategies.
This study aimed to design a multi-epitope vaccine (MEV) against <i>Pasteurella multocida</i> (Pm) using immunoinformatics approaches. Based on four conserved outer membrane proteins (OmpA; OmpH; PlpEand LolA), 15 immunodominant epitopes were identified, including 8 CTL epitopes, 3 HTL epitopes, and 4 B-cell epitopes. ...
41898976
Monitoring of Insecticide Resistance and Resistance-Related Point Mutations in Field-Collected &lt;i&gt;Aphis gossypii&lt;/i&gt; Populations in the Northern Xinjiang, China.
In 2024 and 2025, field populations of <i>Aphis gossypii</i> were collected from eight regions in Xinjiang to monitor their resistance levels to five commonly used insecticides: sulfoxaflor, acetamiprid, imidacloprid, abamectin, and chlorpyrifos. The mutation frequencies of five sites in the acetylcholinesterase (AChE)...
41898300
Targeting T-Cells for Cancer Treatment: Current Clinical Strategies and Challenges.
Modulation of immune response to target tumor cells has been shown to be a successful strategy for cancer treatment. Over the past years immunotherapy has been integrated into cancer treatment and PD-1 blockers have become the backbone of treatment regimens for multiple cancer types. Several classes of immunotherapies,...
41897514
Developmental Toxicity of Chlorinated Polyfluorinated Ether Sulfonate (F-53B), a Perfluorooctane Sulfonate (PFOS) Alternative, in Embryos and Larvae of Blotched Snakehead (&lt;i&gt;Channa maculata&lt;/i&gt;).
Chlorinated polyfluoroalkyl ether sulfonate (F-53B), a common substitute for perfluorooctane sulfonate (PFOS), exhibits similar environmental persistence and bioaccumulation potential, raising concerns about its ecological and health impacts. However, comprehensive toxicological data remain limited for adequate environ...
41897370
Integrating Structural, Biochemical, and Cellular Perspectives on the TFIIH Helicases XPB and XPD.
Xeroderma pigmentosum group B (XPB/ERCC3) and group D (XPD/ERCC2) helicases are integral components of the transcription factor IIH (TFIIH) complex, coordinating DNA unwinding during transcription initiation and nucleotide excision repair (NER). XPB functions as an ATP-driven translocase that generates torsional strain...
41897369
Identification of Ellagic Acid as a Natural GPR35 Agonist for Ulcerative Colitis Therapy.
The escalating global burden of Ulcerative Colitis (UC) underscores the urgent need for novel therapeutic strategies. Although dietary modulation is known to influence UC progression, the specific molecular mediators remain largely undefined. Recently, the G protein coupled receptor 35 (GPR35) has emerged as a promisin...
41892567
Poly(Ionic Liquids) and Ionogels for Electrochromic Devices: Material Design and Additive Manufacturing Strategies.
Escalating requirements for smart energy management are driving advances in functional electrochromic devices (ECDs), which are pivotal for the regulation of light, heat, and reduction in energy consumption in buildings, transportation, and smart devices. However, the commercialization of ECDs is hindered by com plex d...
41892566
High-Performance Gel Design for Flexible Pressure-Sensing Films in Taekwondo Applications.
Exploring effective training methods to reliably trigger scoring in electronic protective gear is a significant challenge faced by coaches and athletes, and it constitutes a critical research direction that urgently demands scientific exploration. To improve the scientific precision of daily Taekwondo training and enha...
41892309
Dual Roles of Ubiquitin-Specific Peptidase 10 (USP10) in Cancer.
Ubiquitin-specific peptidase 10 (USP10) deubiquitinates multiple signaling proteins in cancer cells. These USP10 substrates contain both tumor suppressors and oncogenic proteins, thus conferring both inhibitory and promoting effects of USP10 on tumorigenesis and progression. This review focuses on the dual roles of USP...
41892054
Enhancing the Detection of Long-Chain Aldehydes by Peptide-Based Biosensors Through Counter-Ion Exchange.
Long-chain aldehydes, particularly nonanal, are recognized as potential volatile biomarkers of lung cancer in exhaled breath. This study investigates the influence of peptide counter-ions on the performance of QCM-based biosensors using two odorant-binding protein-derived peptides (OBPP4 and OBPP4 GSGSGS) for the selec...
41892053
Progress in Machine Learning-Assisted Biosensors for Alzheimer's Disease.
Alzheimer's disease (AD) is the most common cause of dementia, affecting 55 million people worldwide. Its characteristics include the accumulation of senile plaques and neurofibrillary tangles. This disease is associated with changes in the concentration of AD biomarkers, such as microRNAs, amyloid peptides, Tau protei...
41892051
Immunosensors and Immunoassays to Detect &lt;i&gt;Francisella tularensis&lt;/i&gt; and Diagnose Tularemia.
<i>Francisella tularensis</i>, the causative agent of tularemia, is a highly infectious Category A biothreat agent characterized by an exceptionally low infectious dose and diverse transmission routes. Due to the pathogen's fastidious growth requirements and the high risk of laboratory-acquired infections, traditional ...
41855810
Effects of hepatic lipid ratio on lipid metabolism indices and hepatic gene expression in late-phase laying hens.
Alleviating hepatic lipid deposition in aging laying hens is critical for maintaining liver health and extending their productive lifespan. This study aimed to identify potential indicators and elucidate the underlying mechanisms associated with liver fat accumulation. Hepatic lipid ratio is a key indicator of the lipi...
41849903
High-sensitivity detection of alpha-fetoprotein via an unlabelled fluorescent probe based on switchable AIEE effect of a phenolic compound.
Alpha-fetoprotein (AFP) is a crucial cancer biomarker for the early screening and diagnosis of hepatocellular carcinoma. Here, we report the discovery of a phenolic compound, 5,5'- (anthracene- 9,10-diyl)bis(benzene-1,3-diol) (ABOL), exhibiting typical aggregation-induced emission enhancement (AIEE) effect. ABOL can bi...
41844083
ROS-responsive puerarin-loaded MOF hydrogel coating: Reprogramming the pathological microenvironment for enhanced diabetic bone regeneration.
The impaired osseointegration of titanium implants in diabetic bone defects is critically challenged by the synergistic interference of oxidative stress, chronic inflammation, and compromised angiogenesis. To address this, this study developed a reactive oxygen species (ROS)-responsive hydrogel coating, termed CS-PA/Mg...
41835786
Bionic Nanoparticle Hydrogel for Astrocyte-Targeted Estradiol Delivery Ameliorates Perimenopausal Depression via P2X7 Receptor Inhibition.
Estrogen (E2) therapy has shown efficacy in alleviating perimenopausal depression but is often accompanied by adverse effects when administered systemically. In this study, we developed a biomimetic nanoliposome hydrogel nasal delivery system for targeted E2 delivery and investigated its mechanisms. The system was cons...
41835183
Machine-Learning-Accelerated Design of Ternary Carrier-Free Nanomedicine for Intranasal Therapy of Brain Metastatic Non-small-cell Lung Cancer.
Non-small-cell lung cancer (NSCLC) with brain metastases poses formidable therapeutic challenges due to acquired resistance and the inherent pharmacokinetic defects of traditional delivery. We developed an innovative lipoic acid-based self-assembled nanodrug (dabrafenib, trametinib, and lipoic acid self-assembly [DTL])...
41835138
Quinoline-functionalized graphene oxide for enhanced cadmium removal: synthesis, characterization, and mechanistic insights.
Cadmium contamination in aquatic systems poses serious environmental and human health risks, driving the need for efficient, selective, and regenerable adsorbents. Herein, graphene oxide (GO) was functionalized with 8-hydroxyquinoline (GQ) and 8-hydroxyquinoline-5-sulfonic acid (GQS) <i>via</i> carbodiimide-mediated co...
41835137
Unleashing the redox-active mediator effect on the Ni-MOF electrode with a flower-like structure employed for supercapacitor applications.
Improving the energy density and performance of a supercapacitor necessitates the use of novel electrode materials and electrolytes that possess long-term stability. The high energy density, pseudocapacitance, and specific capacitance may be conferred by superior electrodes and redox mediator electrolytes used in the p...
41834575
Lancemaside A Derived from &lt;i&gt;Codonopsis lanceolata&lt;/i&gt; Shoots Modulates the Immunostimulatory Responses by Enhancing TLR4 Signaling Pathway in RAW 264.7 Macrophages.
Immune function plays a critical role in defending the body against harmful external stimuli, such as pathogens, and contributes to the maintenance of overall physiological homeostasis. Activation of the immune system is essential for enhancing host defense and preventing immune-related and infectious diseases. In this...
41833837
Crystal structures reveal phosphorylation-dependent disruption of the heat shock protein 70-CHIP interface: A compensatory G132N variant restores binding affinity.
Heat shock protein 70 (HSP70) and its E3 ligase co-chaperone CHIP (STUB1) form a critical quality-control complex that directs client proteins toward folding or degradation. Phosphorylation of HSP70 at a conserved threonine in the C-terminal tail influences the fate of clients during cellular stress, yet the structural...
41830701
Waste upcycling into High-Value MOFs: A Path toward direct Urban mining and sustainable resources recovery.
The rapid growth of industrial activities and urbanisation has generated vast amounts of solid waste, while also increasing the demand for sustainable and functional materials. This highlights the urgent need for strategies that can transform waste streams into valuable resources. Waste streams already contain the esse...
41830661
Amyloid fibril polymorphism: Structural mechanisms of assembly and the links to disease.
Amyloid fibrils are involved in devastating conditions such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and systemic amyloidosis. They exhibit polymorphism, meaning that a single protein sequence can adopt different amyloid folds that vary with time and self-assembly conditions. Polymorphism conf...
41830113
Mimicking The Photosynthetic Special Pair With Aluminum(III) Porphyrin Homodimer - Fullerene (C&lt;sub&gt;60&lt;/sub&gt;) Supramolecular Assemblies Capable of Long-Lived Charge Separation.
The axial-bonding ability of aluminum(III) porphyrin has been employed to construct two covalently linked homodimer systems. The center-to-center distance between the two porphyrins, which are connected via an oxalic bridge is ∼6.90 Å. Unlike most other meso-substituted porphyrins, which have aromatic substituents, the...
41827015
Systematic and precise interventions for KRAS-mutant cancers.
KRAS is the most frequently altered oncogenic driver in human cancer. Its mutations drive the initiation and progression of many solid tumors. Clinical validation of covalent KRAS<sup>G12C</sup> inhibitors marked a step change and renewed focus on allele-directed strategies and the circuits that regulate KRAS signaling...
41826785
Ginkgolide B-cyclodextrin inclusion complexes: an encapsulation strategy for improved solubility and aerosol inhalation therapy in bronchial asthma.
Bronchial asthma represents a long-term inflammatory reaction in the airways, marked by the accumulation of eosinophils. Ginkgolide B (GB), derived from ginkgo biloba, serves as a potent natural antagonist of platelet activating factor (PAF). It can greatly reduce the production and release of eosinophils while minimiz...
41826544
Effects of sialylated oligosaccharides on the N- and O-glycosylation of etanercept in recombinant CHO cells.
In mammalian cell-based bioprocesses, glycosylation of recombinant therapeutic proteins requires stringent control and monitoring as it may affect therapeutic efficacy and safety. The sialylation of glycosylated moieties plays an important role in determining the in vivo clearance of therapeutic proteins by extending t...
41826407
Assessment of Mycobacterium tuberculosis dodecin scaffold as a multimerization platform on the immunogenicity of HPV L2 antigens.
Human papillomavirus infection, the primary cause of cervical cancer (the fourth most common cancer in women), is preventable through prophylactic HPV vaccines. As an alternative to current HPV vaccines, all based on a cocktail of L1 virus-like particles (VLPs) from multiple HPV types, the L2 capsid protein offers cros...
41826298
Impact of solvent forces and broken symmetry on the assembly of designed proteins at a liquid-solid interface.
The era of protein design has enabled the creation of hybrid protein-inorganic interfaces, leading to both surface-directed self-assembly of de novo protein architectures and protein-directed formation of inorganic materials. However, the resulting patterns of protein assembly are often unexpected, implying that essent...
41826253
A Novel Immune-Modulating Nanodrug Enhances Liver-Targeted mRNA Delivery.
Liver-specific delivery of mRNAs encoding key regulatory genes via lipid nanoparticles (LNPs) is promising to restore liver homeostasis and resume liver functions in inflammatory liver diseases. However, inflammation downregulates the global mRNA expression in general as a self-defensive mechanism, e.g., to block viral...
41826232
Visualizing and Quantifying microRNA-Induced DNA Origami Separation at the Nanoscale.
Circulating microRNAs (miRNAs) are promising biomarkers for disease diagnosis, but their small size and instability hinder direct detection. The detection of miRNA using solid-state nanopores typically involves the binding of miRNA to a larger carrier molecule to generate detectable signals. However, these carriers can...
41826075
Proximity Singlet-Oxygen-Activation and Luminescence-Controllable Chemiluminescent Assay for Fast Ultrasensitive Detection of Pathogenic Bacteria.
In this study, a novel proximity singlet oxygen (<sup>1</sup>O<sub>2</sub>)-activation and luminescence-controllable chemiluminescent assay (PALCA) was developed for ultrasensitively detecting pathogenic bacteria. The PALCA system comprised an antimicrobial peptide-coated porphyrin-metal organic framework (pMOF) as a <...
41824955
In Situ Lipid Interactions of an Anticancer Metal Complex.
An integrated multimodal imaging workflow of cryogenic super-resolution fluorescence microscopy and soft X-ray tomography, Orbitrap secondary ion mass spectrometry, and inductively coupled plasma-mass spectrometry has revealed the unexpected targeting of a half-sandwich cyclopentadienyl Rh(III) phenylazopyridine antica...
41824895
Selenium-Integrated MOFs and Their Derived Materials for Advanced Design, Functionality, and Applications.
Selenium-integrated metal-organic frameworks and their derived materials (Se-MOFs) represent a transformative class of materials that synergistically combine the structural tunability of MOFs with Se's unique electronic, catalytic, and biological properties. By confining Se within MOF architectures, Se-MOFs effectively...
41824795
Protection and Delivery of Phytochemicals from Passive Encapsulation to Guaranteed Self-Assembly Induced by Amyloid Template for Chronic Disease Prevention via Modulating Microbial-Host Crosstalk.
The rising incidence of chronic diseases globally has drawn widespread attention to phytochemicals, which exert targeted preventive and alleviating effect by modulating gut microbiota; thus, a potential strategy for precision nutritional interventions is offered. However, many phytochemicals may exhibit relative instab...
41824579
Ground-state orbital angular momentum lasing from liquid crystal torons embedded in a microcavity.
Orbital angular momentum laser beams have many important practical applications, such as optical tweezers or ultrafast communications. Creating such beams represents an important challenge in photonics. Here, we demonstrate that torons, which are topological defects in liquid crystal textures, when embedded in a microc...
41824573
Ionic and adhesive triboelectric elastomer for normalizing charge polarity and density of contact electrification with general material.
Contact electrification (CE) is widely applied in microscale energy harvesting and active sensing. However, the CE results vary in charge density and polarity between different materials, while the preexisted charges on the surface make the CE process more unpredictable. Here, an adhesive triboelectric elastomer (ATE) ...
41824503
Non-Fickian diffusion within assemblies of the intrinsically disordered protein β-casein.
The molecular mechanisms governing internal fluctuations in intrinsically disordered protein (IDP) assemblies are crucial to the stability and dynamics of both regulated and aberrant toxic cellular aggregates, but remain poorly understood. By comprehensively combining high-resolution quasi-elastic neutron scattering wi...