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PMC1421416_F1_4972.jpg | What's the most prominent thing you notice in this picture? | Panel A: Low magnification of the liver biopsy (HE 120 ×). The portal tract (PT) is infiltrated by a mixed inflammatory reaction. Microgranulomatous cell reaction (G) is visible. Panel B: Typical "spider-web" network pattern at hepatic angiography in the setting of partial Budd-Chiari syndrome. Panel C: Abdominal CT scan showing a fresh thrombus in the ostium of the right hepatic vein (arrow). Panel D: Low magnification of liver biopsy showing marked sinusoidal dilatation (S) and congestion around the central vein (CV). PV: portal vein. |
PMC1421416_F1_4971.jpg | What can you see in this picture? | Panel A: Low magnification of the liver biopsy (HE 120 ×). The portal tract (PT) is infiltrated by a mixed inflammatory reaction. Microgranulomatous cell reaction (G) is visible. Panel B: Typical "spider-web" network pattern at hepatic angiography in the setting of partial Budd-Chiari syndrome. Panel C: Abdominal CT scan showing a fresh thrombus in the ostium of the right hepatic vein (arrow). Panel D: Low magnification of liver biopsy showing marked sinusoidal dilatation (S) and congestion around the central vein (CV). PV: portal vein. |
PMC1421416_F1_4969.jpg | What is the core subject represented in this visual? | Panel A: Low magnification of the liver biopsy (HE 120 ×). The portal tract (PT) is infiltrated by a mixed inflammatory reaction. Microgranulomatous cell reaction (G) is visible. Panel B: Typical "spider-web" network pattern at hepatic angiography in the setting of partial Budd-Chiari syndrome. Panel C: Abdominal CT scan showing a fresh thrombus in the ostium of the right hepatic vein (arrow). Panel D: Low magnification of liver biopsy showing marked sinusoidal dilatation (S) and congestion around the central vein (CV). PV: portal vein. |
PMC1421416_F1_4973.jpg | What can you see in this picture? | Panel A: Low magnification of the liver biopsy (HE 120 ×). The portal tract (PT) is infiltrated by a mixed inflammatory reaction. Microgranulomatous cell reaction (G) is visible. Panel B: Typical "spider-web" network pattern at hepatic angiography in the setting of partial Budd-Chiari syndrome. Panel C: Abdominal CT scan showing a fresh thrombus in the ostium of the right hepatic vein (arrow). Panel D: Low magnification of liver biopsy showing marked sinusoidal dilatation (S) and congestion around the central vein (CV). PV: portal vein. |
PMC1421418_F5_4979.jpg | What stands out most in this visual? | 3D FEM models at systole for the three data sets. (a) patient 1, (b) patient 2, (c) and (d) patient 3 at two different time steps in the cardiac cycle. Here a smoothing factor [13] of 20 and a decimation factor [13] of 20 were applied in order to obtain FEM models with a reasonable number of FEM triangles for further processing. 3D FEM model of the same data set as in Figures 3 and 4. The three planes (some with level sets) for each spatial direction show the cutting planes through the model. No smoothing or decimation was applied. |
PMC1421418_F5_4980.jpg | What is the principal component of this image? | 3D FEM models at systole for the three data sets. (a) patient 1, (b) patient 2, (c) and (d) patient 3 at two different time steps in the cardiac cycle. Here a smoothing factor [13] of 20 and a decimation factor [13] of 20 were applied in order to obtain FEM models with a reasonable number of FEM triangles for further processing. 3D FEM model of the same data set as in Figures 3 and 4. The three planes (some with level sets) for each spatial direction show the cutting planes through the model. No smoothing or decimation was applied. |
PMC1421418_F5_4976.jpg | What can you see in this picture? | 3D FEM models at systole for the three data sets. (a) patient 1, (b) patient 2, (c) and (d) patient 3 at two different time steps in the cardiac cycle. Here a smoothing factor [13] of 20 and a decimation factor [13] of 20 were applied in order to obtain FEM models with a reasonable number of FEM triangles for further processing. 3D FEM model of the same data set as in Figures 3 and 4. The three planes (some with level sets) for each spatial direction show the cutting planes through the model. No smoothing or decimation was applied. |
PMC1421418_F5_4978.jpg | What object or scene is depicted here? | 3D FEM models at systole for the three data sets. (a) patient 1, (b) patient 2, (c) and (d) patient 3 at two different time steps in the cardiac cycle. Here a smoothing factor [13] of 20 and a decimation factor [13] of 20 were applied in order to obtain FEM models with a reasonable number of FEM triangles for further processing. 3D FEM model of the same data set as in Figures 3 and 4. The three planes (some with level sets) for each spatial direction show the cutting planes through the model. No smoothing or decimation was applied. |
PMC1421418_F7_4975.jpg | What does this image primarily show? | Rotating 3D model to get the best point of view. This figure illustrates the choice of the best point of view from a single model. (a) The 3D model in the viewer window can be moved and rotated in order to get the best view to the morphological findings. (b) shows the back view. |
PMC1421418_F7_4974.jpg | What is the main focus of this visual representation? | Rotating 3D model to get the best point of view. This figure illustrates the choice of the best point of view from a single model. (a) The 3D model in the viewer window can be moved and rotated in order to get the best view to the morphological findings. (b) shows the back view. |
PMC1421423_F1_4982.jpg | What is the central feature of this picture? | Computed tomography scan during the interferon treatment. Computed tomography scan, performed after the 20th week for DD/DI patient (left) and during the 52nd week for HDI patient (right), confirmed mild ground-glass opacities in both lungs with bronchial wall and interstitial thickening. Sputum cultures were negative for bacteria, fungi, and acid-fast bacilli. |
PMC1421423_F2_4983.jpg | What stands out most in this visual? | Computed tomography scan after the end of the interferon treatment. Computed tomography scan for DD/DI patient (left) and HDI patient (right) performed after the end of treatment showed the disappearance of radiographic infiltrates. |
PMC1421423_F2_4984.jpg | What is the core subject represented in this visual? | Computed tomography scan after the end of the interferon treatment. Computed tomography scan for DD/DI patient (left) and HDI patient (right) performed after the end of treatment showed the disappearance of radiographic infiltrates. |
PMC1421430_F1_4992.jpg | Can you identify the primary element in this image? | Perlecan protein levels in human prostate tumors. Immunohistochemistry of Perlecan protein in prostate cancer (A) and normal prostate (B). Perlecan is present as a secreted protein in the tumor gland lumens (C) but not in the lumens of benign glands or benign corpora amylacea secretions (D). Staining is also seen in metastatic prostate cancer specimens (E). Secondary antibody alone control fails to demonstrate staining (F). All images originally photographed at 400 × magnification. Quantitation of Perlecan mRNA expression by Real Time PCR (G) or protein by digitized dot blot (H) in normal prostate and tumor samples from individual patients presented as fold change in tumor versus normal. Gleason scores for the tumors are listed. Red numbers or columns indicate patients previously shown to have increased expression of SHH, PTCH1 and GLI1 [7]. |
PMC1421430_F1_4991.jpg | Describe the main subject of this image. | Perlecan protein levels in human prostate tumors. Immunohistochemistry of Perlecan protein in prostate cancer (A) and normal prostate (B). Perlecan is present as a secreted protein in the tumor gland lumens (C) but not in the lumens of benign glands or benign corpora amylacea secretions (D). Staining is also seen in metastatic prostate cancer specimens (E). Secondary antibody alone control fails to demonstrate staining (F). All images originally photographed at 400 × magnification. Quantitation of Perlecan mRNA expression by Real Time PCR (G) or protein by digitized dot blot (H) in normal prostate and tumor samples from individual patients presented as fold change in tumor versus normal. Gleason scores for the tumors are listed. Red numbers or columns indicate patients previously shown to have increased expression of SHH, PTCH1 and GLI1 [7]. |
PMC1421430_F1_4995.jpg | What is the core subject represented in this visual? | Perlecan protein levels in human prostate tumors. Immunohistochemistry of Perlecan protein in prostate cancer (A) and normal prostate (B). Perlecan is present as a secreted protein in the tumor gland lumens (C) but not in the lumens of benign glands or benign corpora amylacea secretions (D). Staining is also seen in metastatic prostate cancer specimens (E). Secondary antibody alone control fails to demonstrate staining (F). All images originally photographed at 400 × magnification. Quantitation of Perlecan mRNA expression by Real Time PCR (G) or protein by digitized dot blot (H) in normal prostate and tumor samples from individual patients presented as fold change in tumor versus normal. Gleason scores for the tumors are listed. Red numbers or columns indicate patients previously shown to have increased expression of SHH, PTCH1 and GLI1 [7]. |
PMC1421430_F3_4988.jpg | What is being portrayed in this visual content? | Co-localization of Shh and Perlecan, and correlation with Ki-67 staining. Immunohistochemistry for Sonic Hedgehog (A), demonstrating both weak cytoplasmic staining in prostate cancer epithelial cells and stronger intraluminal staining of secreted SHH. Co-localization of Perlecan (B) and Sonic Hedgehog (C) in consecutive sections of prostate carcinoma. Examples of co-localization of the secreted proteins in gland lumens are highlighted (red asterisks). All histologic images originally photographed at 400 × magnification. Significant co-localization of Perlecan and SHH staining was associated with higher cellular proliferation rates as indicated by Ki-67 nuclear staining by immunohistochemistry (D). |
PMC1431530_F1_4997.jpg | What does this image primarily show? | A Luminescence of tibial cranial muscle and knee joint area after electrotransfer of a luciferase-encoding plasmid and i.p. injection of luciferin. Observation was done 26 minutes after i.p. injection of luciferin (2.5 mg/250 μl) and 7 days after electrotransfer of 3 μg and 60 μg of luciferase encoding plasmid (pC1-luc) into the tibial cranial muscle (on the left) and the knee joint (on the right) respectively. Levels of luminescence are represented in false colours according to a scale from 400 to 1500 (whole scale 65000). 1B and 1C Fluorescence of knee joint after injection of fluorescent albumin. Skin of the leg was removed. Fluorescence was induced with λex = 540 +/- 20 nm and observation was done at λem > 630 nm. Panel B: At these wavelengths, without injecting fluorochrome, it was possible to see bones by transparency. Panel C Just after injection, labelled albumin is concentrated within patella of the knee joint and some diffusion of fluorescence within the joint can be observed. Levels of fluorescence are represented in false colors. |
PMC1431558_F1_5000.jpg | What is being portrayed in this visual content? | (A) Computed tomography (CT) of abdomen showed extra-luminal free air in the peritoneal cavity (white arrow), and marked distension of colonic loop with fecal impaction (black arrow). (B) Fecal impaction with marked colon distention was noted in the distal sigmoid colon (black arrow). |
PMC1431596_F2_5003.jpg | What is the core subject represented in this visual? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F2_5006.jpg | What can you see in this picture? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F2_5007.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F2_5005.jpg | What is the central feature of this picture? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F2_5002.jpg | What is the core subject represented in this visual? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F2_5004.jpg | What key item or scene is captured in this photo? | Immunohistochemical staining for desmin (a,b), mitochondria (c,d) and CD 34 (e,f) comparing before (a,c,e) and after (b,d,f) microwave ablation. Tissues after ablation showed more clear foci of coagulative necrosis with accumulation of mitochondria (thin arrows), and myolysis areas replacing the myofibrillar elements (thick arrows). The majority of the small intramyocardial vessels staining with CD 34 antibodies showed distinct occlusion of their lumens postablation (arrowheads in f). Panels a, b and e are at the same magnification. Panels c and d are at the same magnification. |
PMC1431596_F3_5013.jpg | What can you see in this picture? | Transmission electron photomicrograph of samples preablation (a,c,e) and postablation using microwave (b,d,f) showing the architecture of contractile elements and basement membrane (a,b). The capillary vessel (CV) in (d) shows severe disruption of endothelial and adventitial layers compared with the vessel in preablation sample(c). In panel (e) the extracellular matrix show in preablation an collagen fibers with a fibroblast(open arrow) in the same field. A severely degenerated myocyte in postablation sample shown in (f) has severe disruption of nuclear and basement membranes, disarray and loss of contractile filaments and condensation of heterochromatin. Magnification: (a)x6000 (b)x4800 (c)x3790 (d)5910 (e)x5800 (f)x7540
BM=basement membrane; CF=collagen fibers; MI=mitochondria; MY=myocyte; N=nucleus. |
PMC1431596_F3_5009.jpg | What is the central feature of this picture? | Transmission electron photomicrograph of samples preablation (a,c,e) and postablation using microwave (b,d,f) showing the architecture of contractile elements and basement membrane (a,b). The capillary vessel (CV) in (d) shows severe disruption of endothelial and adventitial layers compared with the vessel in preablation sample(c). In panel (e) the extracellular matrix show in preablation an collagen fibers with a fibroblast(open arrow) in the same field. A severely degenerated myocyte in postablation sample shown in (f) has severe disruption of nuclear and basement membranes, disarray and loss of contractile filaments and condensation of heterochromatin. Magnification: (a)x6000 (b)x4800 (c)x3790 (d)5910 (e)x5800 (f)x7540
BM=basement membrane; CF=collagen fibers; MI=mitochondria; MY=myocyte; N=nucleus. |
PMC1431606_F2_5015.jpg | What is the core subject represented in this visual? | MIBG scintigraphy showing the presence of structural abnormalities involving nerve endings and receptors in a patients after tetralogy of Fallot repair. This findings characterize patients with severe ventricular arrhythmia |
PMC1431606_F2_5018.jpg | What is the dominant medical problem in this image? | MIBG scintigraphy showing the presence of structural abnormalities involving nerve endings and receptors in a patients after tetralogy of Fallot repair. This findings characterize patients with severe ventricular arrhythmia |
PMC1431606_F2_5021.jpg | What does this image primarily show? | MIBG scintigraphy showing the presence of structural abnormalities involving nerve endings and receptors in a patients after tetralogy of Fallot repair. This findings characterize patients with severe ventricular arrhythmia |
PMC1431606_F2_5019.jpg | What's the most prominent thing you notice in this picture? | MIBG scintigraphy showing the presence of structural abnormalities involving nerve endings and receptors in a patients after tetralogy of Fallot repair. This findings characterize patients with severe ventricular arrhythmia |
PMC1431606_F2_5016.jpg | What key item or scene is captured in this photo? | MIBG scintigraphy showing the presence of structural abnormalities involving nerve endings and receptors in a patients after tetralogy of Fallot repair. This findings characterize patients with severe ventricular arrhythmia |
PMC1431606_F4_5022.jpg | What is the dominant medical problem in this image? | Cardiac magnetic resonance with delayed contrast enhancement in a patient after tetralogy of Fallot repair. The areas with fibrosis have an increased signal intensity (white areas, left arrow) as compared with those of healthy myocardium (dark areas, right arrow) |
PMC1431729_F1_5024.jpg | What is the central feature of this picture? | Immunofluorescent staining of the polytene chromosomes. Polytene chromosomes from (a) D. melanogaster and (b) D. virilis are shown. Top left, phase contrast; others as labeled. Panels on the right provide a close-up of the chromocenter and the dot chromosome. In the merge picture, yellow represents equal staining, red represents more H3K9me staining, and green represents more HP1 staining. The dot chromosome is indicated with an arrow. In D. melanogaster, antibodies for HP1 and H3K9me stain both the chromocenter and the dot chromosome, although the HP1 staining is slightly stronger than the H3K9me staining on the dot. In D. virilis, both antibodies stain the chromocenter but neither stains the dot chromosome. |
PMC1431729_F2_5027.jpg | What is the focal point of this photograph? | In situ hybridizations of fosmids to D. virilis polytene chromosomes. Fosmid DNA was labeled and used for in situ hybridization on denatured polytene chromosomes from D. virilis. Three examples are shown (left to right: contigs 106, 72, 113) demonstrating hybridization to a specific band on the dot chromosome (arrowhead). In some cases, signal is associated with the chromocenter, presumably due to repetitive sequences shared with the band on the dot. In situ hybridizations were performed with at least one fosmid from every contig from the dot chromosome with similar results (data not shown). See Table 1 for the chromosome locations of the other fosmids. |
PMC1431729_F2_5025.jpg | What's the most prominent thing you notice in this picture? | In situ hybridizations of fosmids to D. virilis polytene chromosomes. Fosmid DNA was labeled and used for in situ hybridization on denatured polytene chromosomes from D. virilis. Three examples are shown (left to right: contigs 106, 72, 113) demonstrating hybridization to a specific band on the dot chromosome (arrowhead). In some cases, signal is associated with the chromocenter, presumably due to repetitive sequences shared with the band on the dot. In situ hybridizations were performed with at least one fosmid from every contig from the dot chromosome with similar results (data not shown). See Table 1 for the chromosome locations of the other fosmids. |
PMC1434494_pmed-0030151-g002_5029.jpg | What's the most prominent thing you notice in this picture? | Microscopic Characterization of Sectioned Liver Tissue from Patients Who Had Died(A) Light image of a liver tissue section (×100). The central vein is indicated with an arrow.(B) Light image of a liver tissue section (×200).(C) The convergent zone is indicated with an arrow (×100).(D) TEM image of a liver tissue section (×20,000). A bacterium found in the tissue is highlighted with an arrow. |
PMC1434494_pmed-0030151-g002_5031.jpg | Describe the main subject of this image. | Microscopic Characterization of Sectioned Liver Tissue from Patients Who Had Died(A) Light image of a liver tissue section (×100). The central vein is indicated with an arrow.(B) Light image of a liver tissue section (×200).(C) The convergent zone is indicated with an arrow (×100).(D) TEM image of a liver tissue section (×20,000). A bacterium found in the tissue is highlighted with an arrow. |
PMC1434728_F13_5032.jpg | What's the most prominent thing you notice in this picture? | Simulation III – edge-growth with chest-wall trophism. Edge cells remain mitotically active; non-edge cells exit the cell cycle as their distance from the body wall increases (chest-wall trophism). Anterior diaphragm extension is enhanced over Simulation II (Fig. 12), but remains insufficient. The curvilinear shape of the hemi-diaphragm is still not achieved, the CDH defect is not enlarged, and the anterior ipsilateral diaphragm is also affected. |
PMC1434728_F13_5035.jpg | What is the principal component of this image? | Simulation III – edge-growth with chest-wall trophism. Edge cells remain mitotically active; non-edge cells exit the cell cycle as their distance from the body wall increases (chest-wall trophism). Anterior diaphragm extension is enhanced over Simulation II (Fig. 12), but remains insufficient. The curvilinear shape of the hemi-diaphragm is still not achieved, the CDH defect is not enlarged, and the anterior ipsilateral diaphragm is also affected. |
PMC1434728_F13_5033.jpg | What is the central feature of this picture? | Simulation III – edge-growth with chest-wall trophism. Edge cells remain mitotically active; non-edge cells exit the cell cycle as their distance from the body wall increases (chest-wall trophism). Anterior diaphragm extension is enhanced over Simulation II (Fig. 12), but remains insufficient. The curvilinear shape of the hemi-diaphragm is still not achieved, the CDH defect is not enlarged, and the anterior ipsilateral diaphragm is also affected. |
PMC1434730_F1_5036.jpg | Can you identify the primary element in this image? | Honeybee embryonic cells. Embryonic cells (36–40 h.a.o.) grew as single cells or in large clusters. Image captured 2 weeks after initiation. 400 × magnification. |
PMC1434730_F3_5037.jpg | What is being portrayed in this visual content? | Viability of cells studied by use of cell tracer. Viable cells (a) dyed by CFDA SE cell tracer, obtained a green fluorescent appearance (b), which were maintained for more than one month. 400 × magnification. |
PMC1434730_F6_5040.jpg | What stands out most in this visual? | Proliferation in cell clusters. Proliferation occurred in cell clusters (a) arrow) as shown by time laps pictures, a) initial time, b) after 7 hours, c) after 20 hours, d) after 28 hours, e) after 32 hours and f) after 44 hours. 400 × magnification. |
PMC1434730_F6_5042.jpg | What is shown in this image? | Proliferation in cell clusters. Proliferation occurred in cell clusters (a) arrow) as shown by time laps pictures, a) initial time, b) after 7 hours, c) after 20 hours, d) after 28 hours, e) after 32 hours and f) after 44 hours. 400 × magnification. |
PMC1434730_F6_5044.jpg | What is the principal component of this image? | Proliferation in cell clusters. Proliferation occurred in cell clusters (a) arrow) as shown by time laps pictures, a) initial time, b) after 7 hours, c) after 20 hours, d) after 28 hours, e) after 32 hours and f) after 44 hours. 400 × magnification. |
PMC1434730_F6_5039.jpg | What does this image primarily show? | Proliferation in cell clusters. Proliferation occurred in cell clusters (a) arrow) as shown by time laps pictures, a) initial time, b) after 7 hours, c) after 20 hours, d) after 28 hours, e) after 32 hours and f) after 44 hours. 400 × magnification. |
PMC1434730_F6_5041.jpg | What is being portrayed in this visual content? | Proliferation in cell clusters. Proliferation occurred in cell clusters (a) arrow) as shown by time laps pictures, a) initial time, b) after 7 hours, c) after 20 hours, d) after 28 hours, e) after 32 hours and f) after 44 hours. 400 × magnification. |
PMC1434752_F2_5048.jpg | What key item or scene is captured in this photo? | Pathologic changes in the liver (A), kidney (B) and lung (C) of guinea pigs infected with L. interrogans. (HE, magnification × 200). |
PMC1434752_F2_5047.jpg | What is the dominant medical problem in this image? | Pathologic changes in the liver (A), kidney (B) and lung (C) of guinea pigs infected with L. interrogans. (HE, magnification × 200). |
PMC1434752_F2_5046.jpg | What is shown in this image? | Pathologic changes in the liver (A), kidney (B) and lung (C) of guinea pigs infected with L. interrogans. (HE, magnification × 200). |
PMC1434759_F5_5050.jpg | What is shown in this image? | Effect of zoledronate on LPA-induced stress fiber induction, a cellular effect dependent on RhoA geranylgeranylation. On D0, PNT1-A cells were seeded onto glass coverslips in 6-well plates to obtain 60% confluence on D3. On D1, the cells were serum-starved till fixation on D3. After treatment by the indicated drugs then stimulation by LPA (20 μM, 5 hours on D3), actin fibers were visualized by tetramethylrhodamine isothiocyanate-labeled phalloidin. Cells are viewed on a Zeiss Axiophot microscope (X 630), and pictures are taken with a Princeton camera. |
PMC1434759_F5_5049.jpg | What key item or scene is captured in this photo? | Effect of zoledronate on LPA-induced stress fiber induction, a cellular effect dependent on RhoA geranylgeranylation. On D0, PNT1-A cells were seeded onto glass coverslips in 6-well plates to obtain 60% confluence on D3. On D1, the cells were serum-starved till fixation on D3. After treatment by the indicated drugs then stimulation by LPA (20 μM, 5 hours on D3), actin fibers were visualized by tetramethylrhodamine isothiocyanate-labeled phalloidin. Cells are viewed on a Zeiss Axiophot microscope (X 630), and pictures are taken with a Princeton camera. |
PMC1434759_F5_5052.jpg | What stands out most in this visual? | Effect of zoledronate on LPA-induced stress fiber induction, a cellular effect dependent on RhoA geranylgeranylation. On D0, PNT1-A cells were seeded onto glass coverslips in 6-well plates to obtain 60% confluence on D3. On D1, the cells were serum-starved till fixation on D3. After treatment by the indicated drugs then stimulation by LPA (20 μM, 5 hours on D3), actin fibers were visualized by tetramethylrhodamine isothiocyanate-labeled phalloidin. Cells are viewed on a Zeiss Axiophot microscope (X 630), and pictures are taken with a Princeton camera. |
PMC1434759_F5_5051.jpg | What is shown in this image? | Effect of zoledronate on LPA-induced stress fiber induction, a cellular effect dependent on RhoA geranylgeranylation. On D0, PNT1-A cells were seeded onto glass coverslips in 6-well plates to obtain 60% confluence on D3. On D1, the cells were serum-starved till fixation on D3. After treatment by the indicated drugs then stimulation by LPA (20 μM, 5 hours on D3), actin fibers were visualized by tetramethylrhodamine isothiocyanate-labeled phalloidin. Cells are viewed on a Zeiss Axiophot microscope (X 630), and pictures are taken with a Princeton camera. |
PMC1434759_F5_5053.jpg | What is the central feature of this picture? | Effect of zoledronate on LPA-induced stress fiber induction, a cellular effect dependent on RhoA geranylgeranylation. On D0, PNT1-A cells were seeded onto glass coverslips in 6-well plates to obtain 60% confluence on D3. On D1, the cells were serum-starved till fixation on D3. After treatment by the indicated drugs then stimulation by LPA (20 μM, 5 hours on D3), actin fibers were visualized by tetramethylrhodamine isothiocyanate-labeled phalloidin. Cells are viewed on a Zeiss Axiophot microscope (X 630), and pictures are taken with a Princeton camera. |
PMC1434780_F1_5055.jpg | Can you identify the primary element in this image? | Drawing of healthy eyes by a girl from the government school. "Eye are to see with. We can't see without eyes. Eyes are a great blessing of God. Healthy eyes have vision. Eyes have three colours." |
PMC1434780_F3_5057.jpg | What object or scene is depicted here? | Drawing of diseased eyes by a boy from private school. "My eyes are diseased. They remain red all the times. And that is why they water." |
PMC1434780_F4_5056.jpg | Describe the main subject of this image. | Drawing of diseased eyes by a boy from private school. "We can't see well with a diseased eye. A diseased eye is painful." |
PMC1434780_F5_5059.jpg | What's the most prominent thing you notice in this picture? | Drawing of diseased eyes by a boy from government school. "This eye has cataract." |
PMC1434781_F1_5060.jpg | What key item or scene is captured in this photo? | Photograph showing three piece AcrySof MA60BM (Alcon, Fort Worth, TX) optic capture through trypan blue assisted posterior capsulorhexis opening. |
PMC1434781_F2_5061.jpg | What's the most prominent thing you notice in this picture? | Photograph showing three piece AcrySof MA60BM (Alcon, Fort Worth, TX) optic capture through posterior capsulorhexis opening in which trypan blue was not used. |
PMC1434791_ppat-0020026-g002_5065.jpg | What stands out most in this visual? | Pathology in CWD-Infected Animals(A) Histologic lesions of CWD in the dorsal motor nuclei of the vagus nerve within the medulla oblongata of a CWD-affected elk. Note spongiform change, intraneuronal vacuoles, and mild gliosis (hematoxylin and eosin stain, 100× magnification).(B) PrP amyloid deposit stained by immunohistochemistry (brown) and surrounded by vacuoles (original magnification 180×).(C) Perineuronal and extracellular deposits of abnormal PrP (PrPCWD, analogous to PrPSc) in a CWD-affected mule deer (original magnification180×).(D) PrPCWD deposits in the germinal centres of lymphoid follicles in the tonsils of a mule deer (immunohistochemistry, original magnification 50×). |
PMC1434791_ppat-0020026-g002_5062.jpg | What can you see in this picture? | Pathology in CWD-Infected Animals(A) Histologic lesions of CWD in the dorsal motor nuclei of the vagus nerve within the medulla oblongata of a CWD-affected elk. Note spongiform change, intraneuronal vacuoles, and mild gliosis (hematoxylin and eosin stain, 100× magnification).(B) PrP amyloid deposit stained by immunohistochemistry (brown) and surrounded by vacuoles (original magnification 180×).(C) Perineuronal and extracellular deposits of abnormal PrP (PrPCWD, analogous to PrPSc) in a CWD-affected mule deer (original magnification180×).(D) PrPCWD deposits in the germinal centres of lymphoid follicles in the tonsils of a mule deer (immunohistochemistry, original magnification 50×). |
PMC1434791_ppat-0020026-g002_5064.jpg | What is the principal component of this image? | Pathology in CWD-Infected Animals(A) Histologic lesions of CWD in the dorsal motor nuclei of the vagus nerve within the medulla oblongata of a CWD-affected elk. Note spongiform change, intraneuronal vacuoles, and mild gliosis (hematoxylin and eosin stain, 100× magnification).(B) PrP amyloid deposit stained by immunohistochemistry (brown) and surrounded by vacuoles (original magnification 180×).(C) Perineuronal and extracellular deposits of abnormal PrP (PrPCWD, analogous to PrPSc) in a CWD-affected mule deer (original magnification180×).(D) PrPCWD deposits in the germinal centres of lymphoid follicles in the tonsils of a mule deer (immunohistochemistry, original magnification 50×). |
PMC1434791_ppat-0020026-g002_5063.jpg | What is the core subject represented in this visual? | Pathology in CWD-Infected Animals(A) Histologic lesions of CWD in the dorsal motor nuclei of the vagus nerve within the medulla oblongata of a CWD-affected elk. Note spongiform change, intraneuronal vacuoles, and mild gliosis (hematoxylin and eosin stain, 100× magnification).(B) PrP amyloid deposit stained by immunohistochemistry (brown) and surrounded by vacuoles (original magnification 180×).(C) Perineuronal and extracellular deposits of abnormal PrP (PrPCWD, analogous to PrPSc) in a CWD-affected mule deer (original magnification180×).(D) PrPCWD deposits in the germinal centres of lymphoid follicles in the tonsils of a mule deer (immunohistochemistry, original magnification 50×). |
PMC1435760_F1_5066.jpg | Describe the main subject of this image. | (A) T2-weighted and (B) T1-weighted coronal magnetic resonance images (MRI) of a 43 year old man presenting with osteosarcoma involving the proximal humerus (arrows). (C) Antero-posterior and (D) lateral post-operative plain radiographs demonstrating the surgical management, which comprised of a proximal humeral resection and a claviculo-prohumeral reconstruction of the shoulder. |
PMC1435760_F1_5067.jpg | What's the most prominent thing you notice in this picture? | (A) T2-weighted and (B) T1-weighted coronal magnetic resonance images (MRI) of a 43 year old man presenting with osteosarcoma involving the proximal humerus (arrows). (C) Antero-posterior and (D) lateral post-operative plain radiographs demonstrating the surgical management, which comprised of a proximal humeral resection and a claviculo-prohumeral reconstruction of the shoulder. |
PMC1435760_F1_5068.jpg | What is the core subject represented in this visual? | (A) T2-weighted and (B) T1-weighted coronal magnetic resonance images (MRI) of a 43 year old man presenting with osteosarcoma involving the proximal humerus (arrows). (C) Antero-posterior and (D) lateral post-operative plain radiographs demonstrating the surgical management, which comprised of a proximal humeral resection and a claviculo-prohumeral reconstruction of the shoulder. |
PMC1435769_F6_5077.jpg | What object or scene is depicted here? | lipid exchange and syncytia formation during cell-to-cell fusion experiments. HeLa-Env/LAI and target cells were labeled with DiO and DiI, respectively. Co-cultures of labeled cells were performed in the presence of PBS (control), Rgp41A buffer, Rgp41A or T-20. After 6 h, lipid exchange between both types of cells was evaluated by flow cytometry analysis. In parallel, an X-Gal assay was performed to estimate the fusion efficiency between Env- and CD4-expressing cells in the presence or absence of inhibitors. Indicated percentages correspond to the proportion of double-positive gated cells. -: absence of syncytia, +/-: presence of small syncytia, +: presence of many large syncytia. |
PMC1435769_F6_5074.jpg | What is shown in this image? | lipid exchange and syncytia formation during cell-to-cell fusion experiments. HeLa-Env/LAI and target cells were labeled with DiO and DiI, respectively. Co-cultures of labeled cells were performed in the presence of PBS (control), Rgp41A buffer, Rgp41A or T-20. After 6 h, lipid exchange between both types of cells was evaluated by flow cytometry analysis. In parallel, an X-Gal assay was performed to estimate the fusion efficiency between Env- and CD4-expressing cells in the presence or absence of inhibitors. Indicated percentages correspond to the proportion of double-positive gated cells. -: absence of syncytia, +/-: presence of small syncytia, +: presence of many large syncytia. |
PMC1435769_F6_5073.jpg | Can you identify the primary element in this image? | lipid exchange and syncytia formation during cell-to-cell fusion experiments. HeLa-Env/LAI and target cells were labeled with DiO and DiI, respectively. Co-cultures of labeled cells were performed in the presence of PBS (control), Rgp41A buffer, Rgp41A or T-20. After 6 h, lipid exchange between both types of cells was evaluated by flow cytometry analysis. In parallel, an X-Gal assay was performed to estimate the fusion efficiency between Env- and CD4-expressing cells in the presence or absence of inhibitors. Indicated percentages correspond to the proportion of double-positive gated cells. -: absence of syncytia, +/-: presence of small syncytia, +: presence of many large syncytia. |
PMC1435769_F6_5070.jpg | What's the most prominent thing you notice in this picture? | lipid exchange and syncytia formation during cell-to-cell fusion experiments. HeLa-Env/LAI and target cells were labeled with DiO and DiI, respectively. Co-cultures of labeled cells were performed in the presence of PBS (control), Rgp41A buffer, Rgp41A or T-20. After 6 h, lipid exchange between both types of cells was evaluated by flow cytometry analysis. In parallel, an X-Gal assay was performed to estimate the fusion efficiency between Env- and CD4-expressing cells in the presence or absence of inhibitors. Indicated percentages correspond to the proportion of double-positive gated cells. -: absence of syncytia, +/-: presence of small syncytia, +: presence of many large syncytia. |
PMC1435769_F6_5079.jpg | What object or scene is depicted here? | lipid exchange and syncytia formation during cell-to-cell fusion experiments. HeLa-Env/LAI and target cells were labeled with DiO and DiI, respectively. Co-cultures of labeled cells were performed in the presence of PBS (control), Rgp41A buffer, Rgp41A or T-20. After 6 h, lipid exchange between both types of cells was evaluated by flow cytometry analysis. In parallel, an X-Gal assay was performed to estimate the fusion efficiency between Env- and CD4-expressing cells in the presence or absence of inhibitors. Indicated percentages correspond to the proportion of double-positive gated cells. -: absence of syncytia, +/-: presence of small syncytia, +: presence of many large syncytia. |
PMC1435791_ppat-0020028-g001_5081.jpg | What does this image primarily show? | Clinical and Pathological Images of the Patient's Lymph Nodes(A) MRI of the chest showing adenopathy in the precarinal, subcarinal, and hilar regions. Central areas of diminished enhancement suggest necrosis (arrow).(B) Removed cervical lymph node showing necrotizing granulomatous lymphadenitis with abscess formation (pyogranuloma).(C) Warthin-Starry stain of the cervical lymph node (magnification 600×) showing coccobacillary organisms.(D) Higher magnification H&E of a necrotizing granuloma. There is an area of neutrophils and cellular debris on the right, bounded by a poorly defined layer of palisaded epithelioid histiocytes. Outside this layer is a mix of lymphocytes and histiocytes. |
PMC1435791_ppat-0020028-g001_5080.jpg | Can you identify the primary element in this image? | Clinical and Pathological Images of the Patient's Lymph Nodes(A) MRI of the chest showing adenopathy in the precarinal, subcarinal, and hilar regions. Central areas of diminished enhancement suggest necrosis (arrow).(B) Removed cervical lymph node showing necrotizing granulomatous lymphadenitis with abscess formation (pyogranuloma).(C) Warthin-Starry stain of the cervical lymph node (magnification 600×) showing coccobacillary organisms.(D) Higher magnification H&E of a necrotizing granuloma. There is an area of neutrophils and cellular debris on the right, bounded by a poorly defined layer of palisaded epithelioid histiocytes. Outside this layer is a mix of lymphocytes and histiocytes. |
PMC1435791_ppat-0020028-g001_5083.jpg | What is the principal component of this image? | Clinical and Pathological Images of the Patient's Lymph Nodes(A) MRI of the chest showing adenopathy in the precarinal, subcarinal, and hilar regions. Central areas of diminished enhancement suggest necrosis (arrow).(B) Removed cervical lymph node showing necrotizing granulomatous lymphadenitis with abscess formation (pyogranuloma).(C) Warthin-Starry stain of the cervical lymph node (magnification 600×) showing coccobacillary organisms.(D) Higher magnification H&E of a necrotizing granuloma. There is an area of neutrophils and cellular debris on the right, bounded by a poorly defined layer of palisaded epithelioid histiocytes. Outside this layer is a mix of lymphocytes and histiocytes. |
PMC1435791_ppat-0020028-g006_5085.jpg | Describe the main subject of this image. | Histopathology of Mouse Lymph Nodes(A) Normal lymph node architecture of a gp91phox−/− uninjected control mouse (magnification 5×).(B) Normal lymph node architecture of a gp91phox
−/− mouse after 107 i.p. inoculation of Gluconobacter oxydans (magnification 5×).(C) Moderate lymphoid hyperplasia and presence of atypical macrophages in the lymph node of a gp91phox
−/− mouse after 106 intraperitoneal inoculation of the novel organism (magnification 5×).(D) View of the epithelioid macrophages in the lymph node at 40×.(E) Lymphadenitis in the cervical lymph node of a gp91phox
−/− mouse after 107 i.p. inoculation of the novel organism (magnification 5×).(F) View of the pyogranulomatous reaction in the lymph node at 20×.(A,B,E,F) are from mice that were sacrificed at nine days post-inoculation. (C and D) are from a mouse that was sacrificed at 76 days post-inoculation. |
PMC1435791_ppat-0020028-g006_5087.jpg | What can you see in this picture? | Histopathology of Mouse Lymph Nodes(A) Normal lymph node architecture of a gp91phox−/− uninjected control mouse (magnification 5×).(B) Normal lymph node architecture of a gp91phox
−/− mouse after 107 i.p. inoculation of Gluconobacter oxydans (magnification 5×).(C) Moderate lymphoid hyperplasia and presence of atypical macrophages in the lymph node of a gp91phox
−/− mouse after 106 intraperitoneal inoculation of the novel organism (magnification 5×).(D) View of the epithelioid macrophages in the lymph node at 40×.(E) Lymphadenitis in the cervical lymph node of a gp91phox
−/− mouse after 107 i.p. inoculation of the novel organism (magnification 5×).(F) View of the pyogranulomatous reaction in the lymph node at 20×.(A,B,E,F) are from mice that were sacrificed at nine days post-inoculation. (C and D) are from a mouse that was sacrificed at 76 days post-inoculation. |
PMC1435791_ppat-0020028-g006_5084.jpg | Describe the main subject of this image. | Histopathology of Mouse Lymph Nodes(A) Normal lymph node architecture of a gp91phox−/− uninjected control mouse (magnification 5×).(B) Normal lymph node architecture of a gp91phox
−/− mouse after 107 i.p. inoculation of Gluconobacter oxydans (magnification 5×).(C) Moderate lymphoid hyperplasia and presence of atypical macrophages in the lymph node of a gp91phox
−/− mouse after 106 intraperitoneal inoculation of the novel organism (magnification 5×).(D) View of the epithelioid macrophages in the lymph node at 40×.(E) Lymphadenitis in the cervical lymph node of a gp91phox
−/− mouse after 107 i.p. inoculation of the novel organism (magnification 5×).(F) View of the pyogranulomatous reaction in the lymph node at 20×.(A,B,E,F) are from mice that were sacrificed at nine days post-inoculation. (C and D) are from a mouse that was sacrificed at 76 days post-inoculation. |
PMC1435791_ppat-0020028-g006_5086.jpg | Can you identify the primary element in this image? | Histopathology of Mouse Lymph Nodes(A) Normal lymph node architecture of a gp91phox−/− uninjected control mouse (magnification 5×).(B) Normal lymph node architecture of a gp91phox
−/− mouse after 107 i.p. inoculation of Gluconobacter oxydans (magnification 5×).(C) Moderate lymphoid hyperplasia and presence of atypical macrophages in the lymph node of a gp91phox
−/− mouse after 106 intraperitoneal inoculation of the novel organism (magnification 5×).(D) View of the epithelioid macrophages in the lymph node at 40×.(E) Lymphadenitis in the cervical lymph node of a gp91phox
−/− mouse after 107 i.p. inoculation of the novel organism (magnification 5×).(F) View of the pyogranulomatous reaction in the lymph node at 20×.(A,B,E,F) are from mice that were sacrificed at nine days post-inoculation. (C and D) are from a mouse that was sacrificed at 76 days post-inoculation. |
PMC1435874_F4_5090.jpg | What key item or scene is captured in this photo? | Activation of p78MX1 in lymphocytes and epithelial cells in a case of alopecia areata. A, low power field of a scalp biopsy with alopecia areata, showing an inflamed hair follicle (arrow) (H&E). B, low power field of staining with anti-p78MX1 showing positive cells in and around the inflamed hair follicle (solid arrow), but not in an uninvolved region of another hair follicle (dashed arrow). Capillary endothelial cells stain throughout the dermis (asterisks). C, high power field of staining with anti-p78MX1, showing that the p78-positive cells are a subset of lymphocytes (arrow) and outer root sheath epithelial cells (ORS, lines), as well as matrix cells (M) around the dermal papilla (DP). The inner root sheath (IRS) does not stain. A second case of sporadic alopecia areata revealed a similar pattern of staining (data not shown). CTS = connective tissue sheath. |
PMC1435874_F4_5089.jpg | What is the core subject represented in this visual? | Activation of p78MX1 in lymphocytes and epithelial cells in a case of alopecia areata. A, low power field of a scalp biopsy with alopecia areata, showing an inflamed hair follicle (arrow) (H&E). B, low power field of staining with anti-p78MX1 showing positive cells in and around the inflamed hair follicle (solid arrow), but not in an uninvolved region of another hair follicle (dashed arrow). Capillary endothelial cells stain throughout the dermis (asterisks). C, high power field of staining with anti-p78MX1, showing that the p78-positive cells are a subset of lymphocytes (arrow) and outer root sheath epithelial cells (ORS, lines), as well as matrix cells (M) around the dermal papilla (DP). The inner root sheath (IRS) does not stain. A second case of sporadic alopecia areata revealed a similar pattern of staining (data not shown). CTS = connective tissue sheath. |
PMC1435874_F4_5088.jpg | What is being portrayed in this visual content? | Activation of p78MX1 in lymphocytes and epithelial cells in a case of alopecia areata. A, low power field of a scalp biopsy with alopecia areata, showing an inflamed hair follicle (arrow) (H&E). B, low power field of staining with anti-p78MX1 showing positive cells in and around the inflamed hair follicle (solid arrow), but not in an uninvolved region of another hair follicle (dashed arrow). Capillary endothelial cells stain throughout the dermis (asterisks). C, high power field of staining with anti-p78MX1, showing that the p78-positive cells are a subset of lymphocytes (arrow) and outer root sheath epithelial cells (ORS, lines), as well as matrix cells (M) around the dermal papilla (DP). The inner root sheath (IRS) does not stain. A second case of sporadic alopecia areata revealed a similar pattern of staining (data not shown). CTS = connective tissue sheath. |
PMC1435921_F1_5092.jpg | What's the most prominent thing you notice in this picture? | Panel A and B shows a tumor involving the right kidney, measuring 4 × 5 cms. Panel C shows the CD 30 immunostain being positive in the neoplasms cells and Panel D shows a transitional area between renal cell cancer and Hodgkin's lymphoma. |
PMC1435921_F1_5094.jpg | What is the central feature of this picture? | Panel A and B shows a tumor involving the right kidney, measuring 4 × 5 cms. Panel C shows the CD 30 immunostain being positive in the neoplasms cells and Panel D shows a transitional area between renal cell cancer and Hodgkin's lymphoma. |
PMC1435923_F2_5098.jpg | What is the principal component of this image? | Parathyroid hormone (PTH) immunostaining performed on a destained Pap smear (A) and cell block (B) showing distinct cytoplasmic positivity. |
PMC1435923_F2_5097.jpg | Can you identify the primary element in this image? | Parathyroid hormone (PTH) immunostaining performed on a destained Pap smear (A) and cell block (B) showing distinct cytoplasmic positivity. |
PMC1435923_F3_5099.jpg | What object or scene is depicted here? | FNAB smear (A) and core biopsy (B) specimens from a case of metastatic parathyroid carcinoma in the liver showing sheets of relatively bland spindle and round cells. |
PMC1435923_F3_5100.jpg | What is the central feature of this picture? | FNAB smear (A) and core biopsy (B) specimens from a case of metastatic parathyroid carcinoma in the liver showing sheets of relatively bland spindle and round cells. |
PMC1436015_F1_5104.jpg | What is shown in this image? | Anatomical detail visible in ultrasound images. Ultrasound images (A, C) and H&E histological sections (B, D) of implantation sites at E6.5 (A, B) and E7.5 (C, D). Divisions in the scale in A and C are 100 μm apart. The conceptus in histological sections is smaller than in vivo due to shrinkage during tissue preparation (fixation and dehydration). AC, amniotic cavity; Al, allantois; Emb, embryo; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F1_5103.jpg | What is the dominant medical problem in this image? | Anatomical detail visible in ultrasound images. Ultrasound images (A, C) and H&E histological sections (B, D) of implantation sites at E6.5 (A, B) and E7.5 (C, D). Divisions in the scale in A and C are 100 μm apart. The conceptus in histological sections is smaller than in vivo due to shrinkage during tissue preparation (fixation and dehydration). AC, amniotic cavity; Al, allantois; Emb, embryo; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F1_5105.jpg | What's the most prominent thing you notice in this picture? | Anatomical detail visible in ultrasound images. Ultrasound images (A, C) and H&E histological sections (B, D) of implantation sites at E6.5 (A, B) and E7.5 (C, D). Divisions in the scale in A and C are 100 μm apart. The conceptus in histological sections is smaller than in vivo due to shrinkage during tissue preparation (fixation and dehydration). AC, amniotic cavity; Al, allantois; Emb, embryo; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F2_5106.jpg | What does this image primarily show? | Off-target beads following 69 nL microinjection into the amniotic cavity. This E7.5 conceptus was dissected a few hours after ultrasound-guided microinjection of a 69 nL volume containing 3 μm diameter fluorescent beads into the amniotic cavity. (A) The amniotic and yolk sac cavities were visibly distended (e.g. compare to figure 3D) when viewed under a dissection microscope. (B) When the same embryo was viewed under a fluorescent microscope, beads were visible within the amniotic cavity (arrow) but were also present in the adjacent exocoelomic cavity as well as in the ectoplacental cone region. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity; YSC, yolk sac cavity. |
PMC1436015_F2_5107.jpg | What is the focal point of this photograph? | Off-target beads following 69 nL microinjection into the amniotic cavity. This E7.5 conceptus was dissected a few hours after ultrasound-guided microinjection of a 69 nL volume containing 3 μm diameter fluorescent beads into the amniotic cavity. (A) The amniotic and yolk sac cavities were visibly distended (e.g. compare to figure 3D) when viewed under a dissection microscope. (B) When the same embryo was viewed under a fluorescent microscope, beads were visible within the amniotic cavity (arrow) but were also present in the adjacent exocoelomic cavity as well as in the ectoplacental cone region. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity; YSC, yolk sac cavity. |
PMC1436015_F3_5108.jpg | What is being portrayed in this visual content? | Target accuracy. Concepti dissected a few hours after ultrasound-guided microinjections of a 13.8 nL volume containing 3 μm diameter fluorescent beads. In these examples, there were few (e.g. arrows in A&D) or no beads considered off-target (e.g. B&C). The ectoplacental cone region was targeted in (A) at E6.5 and (B) at E7.5. At E7.5, the exocoelomic cavity (C) and amniotic cavity (D) were also targeted. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F3_5111.jpg | Can you identify the primary element in this image? | Target accuracy. Concepti dissected a few hours after ultrasound-guided microinjections of a 13.8 nL volume containing 3 μm diameter fluorescent beads. In these examples, there were few (e.g. arrows in A&D) or no beads considered off-target (e.g. B&C). The ectoplacental cone region was targeted in (A) at E6.5 and (B) at E7.5. At E7.5, the exocoelomic cavity (C) and amniotic cavity (D) were also targeted. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F3_5110.jpg | What can you see in this picture? | Target accuracy. Concepti dissected a few hours after ultrasound-guided microinjections of a 13.8 nL volume containing 3 μm diameter fluorescent beads. In these examples, there were few (e.g. arrows in A&D) or no beads considered off-target (e.g. B&C). The ectoplacental cone region was targeted in (A) at E6.5 and (B) at E7.5. At E7.5, the exocoelomic cavity (C) and amniotic cavity (D) were also targeted. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F3_5109.jpg | What is the focal point of this photograph? | Target accuracy. Concepti dissected a few hours after ultrasound-guided microinjections of a 13.8 nL volume containing 3 μm diameter fluorescent beads. In these examples, there were few (e.g. arrows in A&D) or no beads considered off-target (e.g. B&C). The ectoplacental cone region was targeted in (A) at E6.5 and (B) at E7.5. At E7.5, the exocoelomic cavity (C) and amniotic cavity (D) were also targeted. AC, amniotic cavity; EPC, ectoplacental cone region; Exo, exocoelomic cavity. |
PMC1436015_F4_5113.jpg | What can you see in this picture? | Histological detection of injected beads. Implantation site (tissue autofluoresces orange) containing green fluorescent beads collected a few hours following ultrasound-guided microinjection into the ectoplacental cone region at E7.5. Beads visualized in 50 μm frozen sections were primarily localized to the targeted ectoplacental cone region. AC, amniotic cavity; Emb, embryo; EPC, ectoplacental cone; Exo, exocoelomic cavity; YSC, yolk sac cavity. |
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