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PMC1457013_pbio-0040158-g006_5393.jpg | What object or scene is depicted here? | Premotor Cortical Areas with Increased BOLD Responses with Both the Left- and the Right-Hand Map Overlaid on Coronal Slices of the MNI T1-Weighted Brain TemplateFor the left hemisphere (L), significant activations (
p < 0.01, FWE-corrected) occurred in one cluster (443 voxels) with two maxima in precentral gyrus (#2 and #4; BA 6), in one single-peak cluster (153 voxels) in superior frontal gyrus (#3; BA 6), in one cluster (281 voxels) with two maxima in medial frontal gyrus (#5 and #6; BA 6), and in one small single-peak cluster (29 voxels) in left inferior frontal gyrus (#1; BA 44). In the right hemisphere (R), there was one cluster (303 voxels) with three maxima, one of which was located in the precentral gyrus outside the cluster delineated by left-hand-map > right-hand-map contrast (#7; BA 6). Solid black lines in the left and right hemisphere outline the clusters identified with the right-hand-map > left-hand-map and left-hand-map > right-hand-map contrasts, respectively (see
Figure 4C). Histograms give percent BOLD signal change relative to mean of session for the local maxima of identified clusters. Red and blue columns refer to left- and right-hand maps, respectively. Column height gives data averaged across participants and error bar ± 1SEM (
n = 16). Coordinates (X, Y, Z in MNI stereotaxic space) and
t
(30) values for the maxima are presented below each histogram.
|
PMC1458331_F1_5396.jpg | What is the central feature of this picture? | Criteria used when counting fibrocytes in bronchial biopsies. Sections from patients and controls were based on an intact basement membrane together with intact tissue of at least 70 μm in every section. Area A starts were the basement membrane ends. A = 0–70 μm in the section. B = 71–140 μm in the section and C = 141–210 μm in the tissue. Definition of abbreviations: BM = Basement membrane, E = epithelium, MF = Muscle fibers. |
PMC1458331_F1_5395.jpg | Describe the main subject of this image. | Criteria used when counting fibrocytes in bronchial biopsies. Sections from patients and controls were based on an intact basement membrane together with intact tissue of at least 70 μm in every section. Area A starts were the basement membrane ends. A = 0–70 μm in the section. B = 71–140 μm in the section and C = 141–210 μm in the tissue. Definition of abbreviations: BM = Basement membrane, E = epithelium, MF = Muscle fibers. |
PMC1458331_F3_5399.jpg | What is the dominant medical problem in this image? | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458331_F3_5402.jpg | Describe the main subject of this image. | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458331_F3_5397.jpg | What does this image primarily show? | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458331_F3_5404.jpg | What stands out most in this visual? | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458331_F3_5400.jpg | Can you identify the primary element in this image? | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458331_F3_5403.jpg | What is the core subject represented in this visual? | Fibrocytes in patients with mild asthma express collagen. Bronchial biopsies were stained using immunofluorescent antibodies against prolyl-4-hydroxylase (green) CD45RO (red) and nuclei (blue) (A). Squares indicate the same cluster area as shown in A. The merged picture describes the positive cells within the square. Cells were further stained for CD34 (green), procollagen I (red), and nuclei (blue) (B), and were subjected to confocal microscopy. The merged picture describes the positive cell within the square. Definition of abbreviations: BM = Basement membrane |
PMC1458342_F1_5405.jpg | What key item or scene is captured in this photo? | Computed tomography: well delimited pulmonary nodule of 13 mm in diameter of the LUL. |
PMC1458347_F3_5409.jpg | What is the core subject represented in this visual? | A) Lateral graphy, B) Coronal 3D reconstruction computed tomography, C) Sagittal reformate image: height loss of L4 vertebrae, narrowing disc space with end plates destruction. D) Postoperative lateral graphy: improvement of vertebral axis and disc space. |
PMC1458347_F3_5407.jpg | What stands out most in this visual? | A) Lateral graphy, B) Coronal 3D reconstruction computed tomography, C) Sagittal reformate image: height loss of L4 vertebrae, narrowing disc space with end plates destruction. D) Postoperative lateral graphy: improvement of vertebral axis and disc space. |
PMC1458347_F3_5408.jpg | What is the principal component of this image? | A) Lateral graphy, B) Coronal 3D reconstruction computed tomography, C) Sagittal reformate image: height loss of L4 vertebrae, narrowing disc space with end plates destruction. D) Postoperative lateral graphy: improvement of vertebral axis and disc space. |
PMC1458348_F1_5406.jpg | What's the most prominent thing you notice in this picture? | A 2-dimensional transesophageal long-axis echocardiogram showing a mobile, pediculated echodense image adjacent to the lower intraventricular septum. |
PMC1458354_F1_5410.jpg | What object or scene is depicted here? | Coronal paranasal computerized tomography demonstrating a frontal mucocele and an intra orbital cystic mass having similar intensities and a suspected area of communication through the bony roof of the orbit. |
PMC1458354_F1_5411.jpg | What can you see in this picture? | Coronal paranasal computerized tomography demonstrating a frontal mucocele and an intra orbital cystic mass having similar intensities and a suspected area of communication through the bony roof of the orbit. |
PMC1458354_F3_5413.jpg | What can you see in this picture? | Axial magnetic resonance imaging demonstrating avert proptosis and lateral displacement of the orbit. |
PMC1458359_F1_5414.jpg | What object or scene is depicted here? | Thrombus in left ventricular apex. Thrombus noted in left ventricular apex (white area in non-contrast enhanced image on left, black lucent area in contrast enhanced image on right) |
PMC1458359_F1_5415.jpg | What is the principal component of this image? | Thrombus in left ventricular apex. Thrombus noted in left ventricular apex (white area in non-contrast enhanced image on left, black lucent area in contrast enhanced image on right) |
PMC1458959_ppat-0020041-g001_5416.jpg | What is the focal point of this photograph? | APOBEC3G Localizes to Cytoplasmic BodiesSubcellular localization images of living 293T cells transiently expressing APO3G-YFP (A), HeLa cells transiently expressing APO3G-YFP (B), HeLa-APO3G cells stably expressing APO3G-Myc (C), endogenous APOBEC3G in primary peripheral blood CD4+ T cells (D), and endogenous APOBEC3G in H9 T lymphocytes (E). APO3G-YFP was detected by direct YFP fluorescence while APO3G-Myc and endogenous APOBEC3G were detected by indirect immunostaining, using antibodies against the c-Myc epitope and APOBEC3G, respectively. The cells were stained with Hoechst 33258 to visualize nuclei and the imaged were merged digitally. Cell type is noted to the left of each image and arrows point to cytoplasmic bodies. |
PMC1458959_ppat-0020041-g001_5420.jpg | What key item or scene is captured in this photo? | APOBEC3G Localizes to Cytoplasmic BodiesSubcellular localization images of living 293T cells transiently expressing APO3G-YFP (A), HeLa cells transiently expressing APO3G-YFP (B), HeLa-APO3G cells stably expressing APO3G-Myc (C), endogenous APOBEC3G in primary peripheral blood CD4+ T cells (D), and endogenous APOBEC3G in H9 T lymphocytes (E). APO3G-YFP was detected by direct YFP fluorescence while APO3G-Myc and endogenous APOBEC3G were detected by indirect immunostaining, using antibodies against the c-Myc epitope and APOBEC3G, respectively. The cells were stained with Hoechst 33258 to visualize nuclei and the imaged were merged digitally. Cell type is noted to the left of each image and arrows point to cytoplasmic bodies. |
PMC1458959_ppat-0020041-g001_5417.jpg | Can you identify the primary element in this image? | APOBEC3G Localizes to Cytoplasmic BodiesSubcellular localization images of living 293T cells transiently expressing APO3G-YFP (A), HeLa cells transiently expressing APO3G-YFP (B), HeLa-APO3G cells stably expressing APO3G-Myc (C), endogenous APOBEC3G in primary peripheral blood CD4+ T cells (D), and endogenous APOBEC3G in H9 T lymphocytes (E). APO3G-YFP was detected by direct YFP fluorescence while APO3G-Myc and endogenous APOBEC3G were detected by indirect immunostaining, using antibodies against the c-Myc epitope and APOBEC3G, respectively. The cells were stained with Hoechst 33258 to visualize nuclei and the imaged were merged digitally. Cell type is noted to the left of each image and arrows point to cytoplasmic bodies. |
PMC1458959_ppat-0020041-g001_5419.jpg | What is the principal component of this image? | APOBEC3G Localizes to Cytoplasmic BodiesSubcellular localization images of living 293T cells transiently expressing APO3G-YFP (A), HeLa cells transiently expressing APO3G-YFP (B), HeLa-APO3G cells stably expressing APO3G-Myc (C), endogenous APOBEC3G in primary peripheral blood CD4+ T cells (D), and endogenous APOBEC3G in H9 T lymphocytes (E). APO3G-YFP was detected by direct YFP fluorescence while APO3G-Myc and endogenous APOBEC3G were detected by indirect immunostaining, using antibodies against the c-Myc epitope and APOBEC3G, respectively. The cells were stained with Hoechst 33258 to visualize nuclei and the imaged were merged digitally. Cell type is noted to the left of each image and arrows point to cytoplasmic bodies. |
PMC1458959_ppat-0020041-g001_5418.jpg | What is the central feature of this picture? | APOBEC3G Localizes to Cytoplasmic BodiesSubcellular localization images of living 293T cells transiently expressing APO3G-YFP (A), HeLa cells transiently expressing APO3G-YFP (B), HeLa-APO3G cells stably expressing APO3G-Myc (C), endogenous APOBEC3G in primary peripheral blood CD4+ T cells (D), and endogenous APOBEC3G in H9 T lymphocytes (E). APO3G-YFP was detected by direct YFP fluorescence while APO3G-Myc and endogenous APOBEC3G were detected by indirect immunostaining, using antibodies against the c-Myc epitope and APOBEC3G, respectively. The cells were stained with Hoechst 33258 to visualize nuclei and the imaged were merged digitally. Cell type is noted to the left of each image and arrows point to cytoplasmic bodies. |
PMC1458959_ppat-0020041-g002_5434.jpg | What is the core subject represented in this visual? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5440.jpg | What key item or scene is captured in this photo? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5432.jpg | What is the principal component of this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5426.jpg | What is the core subject represented in this visual? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5422.jpg | What is the central feature of this picture? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5431.jpg | What is the principal component of this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5433.jpg | What is the core subject represented in this visual? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5424.jpg | What is the core subject represented in this visual? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5435.jpg | What is the principal component of this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5438.jpg | What is the principal component of this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5428.jpg | What can you see in this picture? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5430.jpg | What's the most prominent thing you notice in this picture? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5439.jpg | What is the central feature of this picture? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5423.jpg | What is the dominant medical problem in this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g002_5427.jpg | What is the principal component of this image? | APOBEC3G Cytoplasmic Bodies Are P-Bodies(A) Subcellular localization of APOBEC3G and LSM1 in T cells. Endogenous APOBEC3G and LSM1 were localized in peripheral blood CD4+ T cells (a) and H9 T lymphocytes (b) through indirect immunostaining using antibodies against APOBEC3G and LSM1, respectively. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. A corresponding differential interference contrast (DIC) image is presented to the left and arrows point to cytoplasmic bodies.(B) APOBEC3G colocalizes with P-body proteins at P-bodies. Subcellular localization of APO3G-Myc and YFP-LSM1 (a), YFP-AGO2 (b), YFP-eIF4E (c), YFP-eIF4E-T (d), YFP-RCK/p54 (e), or YFP-DCP2 (f) in HeLa-APO3G cells. APO3G-Myc was detected through indirect immunostaining with an antibody against the c-Myc epitope and YFP was detected by direct fluorescence. The cells were counterstained with Hoechst 33258 to visualize nuclei and the images were digitally merged to highlight regions of colocalization, which appear white in the merged images. Arrows point to P-bodies. |
PMC1458959_ppat-0020041-g005_5443.jpg | What is the central feature of this picture? | Localization of Vif to P-Bodies Requires APOBEC3G(A) Myc-AGO2 is not sensitive to Vif-mediated degradation in the absence or presence of APO3G-CFP. Total cell extracts from 293T cells expressing CFP or APO3G-CFP with Myc-AGO2 and either NL-A1Δvif or NL-A1 were analyzed by immunoblot with antibodies against CFP (α-GFP), c-Myc (α-Myc) and Vif (α-Vif). Numbers to the left indicate molecular mass in kDa.(B) Subcellular localization images of 293T cells coexpressing a combination of Myc-AGO2 and VifC114S (a), APO3G-CFP, Myc-AGO2, and VifC114S (b), or APO3G-CFP, Myc-AGO2, and Vif following a 4-h incubation with the proteasome inhibition ALLN (d) or the equivalent DMSO vehicle control–treated cells (c). APO3G-CFP was detected by direct CFP fluorescence while Myc-AGO2 and Vif protein were detected by indirect immunostaining with antibodies against c-Myc and Vif, respectively. Arrows mark regions of colocalization or lack of colocalization between proteins. |
PMC1458959_ppat-0020041-g005_5445.jpg | What is the focal point of this photograph? | Localization of Vif to P-Bodies Requires APOBEC3G(A) Myc-AGO2 is not sensitive to Vif-mediated degradation in the absence or presence of APO3G-CFP. Total cell extracts from 293T cells expressing CFP or APO3G-CFP with Myc-AGO2 and either NL-A1Δvif or NL-A1 were analyzed by immunoblot with antibodies against CFP (α-GFP), c-Myc (α-Myc) and Vif (α-Vif). Numbers to the left indicate molecular mass in kDa.(B) Subcellular localization images of 293T cells coexpressing a combination of Myc-AGO2 and VifC114S (a), APO3G-CFP, Myc-AGO2, and VifC114S (b), or APO3G-CFP, Myc-AGO2, and Vif following a 4-h incubation with the proteasome inhibition ALLN (d) or the equivalent DMSO vehicle control–treated cells (c). APO3G-CFP was detected by direct CFP fluorescence while Myc-AGO2 and Vif protein were detected by indirect immunostaining with antibodies against c-Myc and Vif, respectively. Arrows mark regions of colocalization or lack of colocalization between proteins. |
PMC1458959_ppat-0020041-g005_5442.jpg | What can you see in this picture? | Localization of Vif to P-Bodies Requires APOBEC3G(A) Myc-AGO2 is not sensitive to Vif-mediated degradation in the absence or presence of APO3G-CFP. Total cell extracts from 293T cells expressing CFP or APO3G-CFP with Myc-AGO2 and either NL-A1Δvif or NL-A1 were analyzed by immunoblot with antibodies against CFP (α-GFP), c-Myc (α-Myc) and Vif (α-Vif). Numbers to the left indicate molecular mass in kDa.(B) Subcellular localization images of 293T cells coexpressing a combination of Myc-AGO2 and VifC114S (a), APO3G-CFP, Myc-AGO2, and VifC114S (b), or APO3G-CFP, Myc-AGO2, and Vif following a 4-h incubation with the proteasome inhibition ALLN (d) or the equivalent DMSO vehicle control–treated cells (c). APO3G-CFP was detected by direct CFP fluorescence while Myc-AGO2 and Vif protein were detected by indirect immunostaining with antibodies against c-Myc and Vif, respectively. Arrows mark regions of colocalization or lack of colocalization between proteins. |
PMC1458959_ppat-0020041-g005_5441.jpg | What key item or scene is captured in this photo? | Localization of Vif to P-Bodies Requires APOBEC3G(A) Myc-AGO2 is not sensitive to Vif-mediated degradation in the absence or presence of APO3G-CFP. Total cell extracts from 293T cells expressing CFP or APO3G-CFP with Myc-AGO2 and either NL-A1Δvif or NL-A1 were analyzed by immunoblot with antibodies against CFP (α-GFP), c-Myc (α-Myc) and Vif (α-Vif). Numbers to the left indicate molecular mass in kDa.(B) Subcellular localization images of 293T cells coexpressing a combination of Myc-AGO2 and VifC114S (a), APO3G-CFP, Myc-AGO2, and VifC114S (b), or APO3G-CFP, Myc-AGO2, and Vif following a 4-h incubation with the proteasome inhibition ALLN (d) or the equivalent DMSO vehicle control–treated cells (c). APO3G-CFP was detected by direct CFP fluorescence while Myc-AGO2 and Vif protein were detected by indirect immunostaining with antibodies against c-Myc and Vif, respectively. Arrows mark regions of colocalization or lack of colocalization between proteins. |
PMC1458960_ppat-0020039-g007_5449.jpg | What is shown in this image? | Electron Microscopy Examination of HIV-1 Assembly and Effects of Vpu and Endocytosis InhibitionHela cells expressing Gag-Pol in the presence or absence of Vpu or Rab5a(S34N) were examined.(A–C) Examples of cells expressing Gag-Pol only, revealing mature particles at both the PM (A and C) and within internal, apparently membrane-bound compartments (arrows in A and B). Immature budding structure are also observed at the PM but only rarely within endosomes.(D) Cells co-expressing Gag-Pol and Vpu with reduced numbers of cell-associated particles, and absence of particles from endosomes.(E and F) Cells co-expressing Gag-Pol and Rab5a(S34N), with exclusively surface-accumulated virions. |
PMC1458960_ppat-0020039-g007_5452.jpg | What is the main focus of this visual representation? | Electron Microscopy Examination of HIV-1 Assembly and Effects of Vpu and Endocytosis InhibitionHela cells expressing Gag-Pol in the presence or absence of Vpu or Rab5a(S34N) were examined.(A–C) Examples of cells expressing Gag-Pol only, revealing mature particles at both the PM (A and C) and within internal, apparently membrane-bound compartments (arrows in A and B). Immature budding structure are also observed at the PM but only rarely within endosomes.(D) Cells co-expressing Gag-Pol and Vpu with reduced numbers of cell-associated particles, and absence of particles from endosomes.(E and F) Cells co-expressing Gag-Pol and Rab5a(S34N), with exclusively surface-accumulated virions. |
PMC1458960_ppat-0020039-g007_5448.jpg | What is shown in this image? | Electron Microscopy Examination of HIV-1 Assembly and Effects of Vpu and Endocytosis InhibitionHela cells expressing Gag-Pol in the presence or absence of Vpu or Rab5a(S34N) were examined.(A–C) Examples of cells expressing Gag-Pol only, revealing mature particles at both the PM (A and C) and within internal, apparently membrane-bound compartments (arrows in A and B). Immature budding structure are also observed at the PM but only rarely within endosomes.(D) Cells co-expressing Gag-Pol and Vpu with reduced numbers of cell-associated particles, and absence of particles from endosomes.(E and F) Cells co-expressing Gag-Pol and Rab5a(S34N), with exclusively surface-accumulated virions. |
PMC1458960_ppat-0020039-g007_5447.jpg | What is the main focus of this visual representation? | Electron Microscopy Examination of HIV-1 Assembly and Effects of Vpu and Endocytosis InhibitionHela cells expressing Gag-Pol in the presence or absence of Vpu or Rab5a(S34N) were examined.(A–C) Examples of cells expressing Gag-Pol only, revealing mature particles at both the PM (A and C) and within internal, apparently membrane-bound compartments (arrows in A and B). Immature budding structure are also observed at the PM but only rarely within endosomes.(D) Cells co-expressing Gag-Pol and Vpu with reduced numbers of cell-associated particles, and absence of particles from endosomes.(E and F) Cells co-expressing Gag-Pol and Rab5a(S34N), with exclusively surface-accumulated virions. |
PMC1458960_ppat-0020039-g007_5451.jpg | What is the dominant medical problem in this image? | Electron Microscopy Examination of HIV-1 Assembly and Effects of Vpu and Endocytosis InhibitionHela cells expressing Gag-Pol in the presence or absence of Vpu or Rab5a(S34N) were examined.(A–C) Examples of cells expressing Gag-Pol only, revealing mature particles at both the PM (A and C) and within internal, apparently membrane-bound compartments (arrows in A and B). Immature budding structure are also observed at the PM but only rarely within endosomes.(D) Cells co-expressing Gag-Pol and Vpu with reduced numbers of cell-associated particles, and absence of particles from endosomes.(E and F) Cells co-expressing Gag-Pol and Rab5a(S34N), with exclusively surface-accumulated virions. |
PMC1459123_F4_5456.jpg | Can you identify the primary element in this image? | Image guidance was based on axial planar views (sagittal, coronal and transaxial), free planar views, and 3D views of the anatomical objects with tools for targeting and trajectory planning. |
PMC1459123_F4_5455.jpg | What is the focal point of this photograph? | Image guidance was based on axial planar views (sagittal, coronal and transaxial), free planar views, and 3D views of the anatomical objects with tools for targeting and trajectory planning. |
PMC1459123_F4_5454.jpg | What is shown in this image? | Image guidance was based on axial planar views (sagittal, coronal and transaxial), free planar views, and 3D views of the anatomical objects with tools for targeting and trajectory planning. |
PMC1459145_F3_5458.jpg | What's the most prominent thing you notice in this picture? | Photographs of micro-cytopathic effect neutralization tests. The F(ab')2 against H5N1 virus was diluted into two-fold serial dilutions, and incubated with an equal volume of active H5N1 virus dilution (100 TCID50). After neutralization, each mixture was added to MDCK cell monolayers in micro-plates, and incubated at 37°C to observe CPE status. These photographs showed the morphologic changes of MDCK cells at 72 h after infection. (A) Cell control (no CPE); (B) cell morphologic changes infected with the H5N1 virus; (C) MDCK cells protected from infection of H5N1 virus by anti-H5N1 F(ab')2. |
PMC1459145_F3_5460.jpg | What is the main focus of this visual representation? | Photographs of micro-cytopathic effect neutralization tests. The F(ab')2 against H5N1 virus was diluted into two-fold serial dilutions, and incubated with an equal volume of active H5N1 virus dilution (100 TCID50). After neutralization, each mixture was added to MDCK cell monolayers in micro-plates, and incubated at 37°C to observe CPE status. These photographs showed the morphologic changes of MDCK cells at 72 h after infection. (A) Cell control (no CPE); (B) cell morphologic changes infected with the H5N1 virus; (C) MDCK cells protected from infection of H5N1 virus by anti-H5N1 F(ab')2. |
PMC1459145_F3_5459.jpg | What is the focal point of this photograph? | Photographs of micro-cytopathic effect neutralization tests. The F(ab')2 against H5N1 virus was diluted into two-fold serial dilutions, and incubated with an equal volume of active H5N1 virus dilution (100 TCID50). After neutralization, each mixture was added to MDCK cell monolayers in micro-plates, and incubated at 37°C to observe CPE status. These photographs showed the morphologic changes of MDCK cells at 72 h after infection. (A) Cell control (no CPE); (B) cell morphologic changes infected with the H5N1 virus; (C) MDCK cells protected from infection of H5N1 virus by anti-H5N1 F(ab')2. |
PMC1459147_F1_5469.jpg | What is shown in this image? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5466.jpg | What does this image primarily show? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5471.jpg | What is the central feature of this picture? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5462.jpg | Describe the main subject of this image. | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5470.jpg | What stands out most in this visual? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5467.jpg | What stands out most in this visual? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5463.jpg | What is the focal point of this photograph? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5465.jpg | What is the main focus of this visual representation? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5461.jpg | What is the principal component of this image? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F1_5472.jpg | What key item or scene is captured in this photo? | Distribution of WFDC2 in the respiratory tract and salivary glands. Immunohistochemistry for WFDC2 was performed as described in materials and methods. Sections show staining in nasal antral mucosa (A, B), nasal polyps (C), airway sub-mucosal glands (D, E), epithelial cells of the airways (F) and negative staining in peripheral lung (G). Staining was also found in parotid (H), submandibular (I) and sub-lingual (J) glands and minor glands from the tongue (K) and tonsil (L). The original magnification of the images was ×100 (A, B, C, D), ×200 (E, F, H, I, J) and ×400 (G, K, L). |
PMC1459147_F2_5477.jpg | What is the dominant medical problem in this image? | WFDC2 and SLPI do not co-localise in major and minor glands. The expression of WFDC2 (A, D) was directly compared with that of the related 2-WAP domain containing protein SLPI (B, E) and the mucous cell marker SPLUNC1 (C, F) in multiple glandular tissues as described in the materials and methods. Sections shown are submandibular gland (A-C) and bronchial sub-mucosal glands (D-F). The original magnification of the images was ×200. |
PMC1459147_F2_5476.jpg | What key item or scene is captured in this photo? | WFDC2 and SLPI do not co-localise in major and minor glands. The expression of WFDC2 (A, D) was directly compared with that of the related 2-WAP domain containing protein SLPI (B, E) and the mucous cell marker SPLUNC1 (C, F) in multiple glandular tissues as described in the materials and methods. Sections shown are submandibular gland (A-C) and bronchial sub-mucosal glands (D-F). The original magnification of the images was ×200. |
PMC1459147_F2_5475.jpg | What is the dominant medical problem in this image? | WFDC2 and SLPI do not co-localise in major and minor glands. The expression of WFDC2 (A, D) was directly compared with that of the related 2-WAP domain containing protein SLPI (B, E) and the mucous cell marker SPLUNC1 (C, F) in multiple glandular tissues as described in the materials and methods. Sections shown are submandibular gland (A-C) and bronchial sub-mucosal glands (D-F). The original magnification of the images was ×200. |
PMC1459147_F2_5473.jpg | Describe the main subject of this image. | WFDC2 and SLPI do not co-localise in major and minor glands. The expression of WFDC2 (A, D) was directly compared with that of the related 2-WAP domain containing protein SLPI (B, E) and the mucous cell marker SPLUNC1 (C, F) in multiple glandular tissues as described in the materials and methods. Sections shown are submandibular gland (A-C) and bronchial sub-mucosal glands (D-F). The original magnification of the images was ×200. |
PMC1459147_F3_5484.jpg | What is the main focus of this visual representation? | WFDC2 is abnormally expressed in the Cystic Fibrosis lung. Sections of normal (A, B) and Cystic Fibrosis lung (C – I) were stained with WFDC2 (A, C, D, G), SLPI (B, E, H) and mucin 5AC (F, I) as described in materials and methods. The original magnification of the images was ×100 (A, B) and ×200 (C, D, E, F, G, H, I). |
PMC1459147_F3_5483.jpg | What key item or scene is captured in this photo? | WFDC2 is abnormally expressed in the Cystic Fibrosis lung. Sections of normal (A, B) and Cystic Fibrosis lung (C – I) were stained with WFDC2 (A, C, D, G), SLPI (B, E, H) and mucin 5AC (F, I) as described in materials and methods. The original magnification of the images was ×100 (A, B) and ×200 (C, D, E, F, G, H, I). |
PMC1459147_F3_5479.jpg | What does this image primarily show? | WFDC2 is abnormally expressed in the Cystic Fibrosis lung. Sections of normal (A, B) and Cystic Fibrosis lung (C – I) were stained with WFDC2 (A, C, D, G), SLPI (B, E, H) and mucin 5AC (F, I) as described in materials and methods. The original magnification of the images was ×100 (A, B) and ×200 (C, D, E, F, G, H, I). |
PMC1459147_F3_5481.jpg | What is the dominant medical problem in this image? | WFDC2 is abnormally expressed in the Cystic Fibrosis lung. Sections of normal (A, B) and Cystic Fibrosis lung (C – I) were stained with WFDC2 (A, C, D, G), SLPI (B, E, H) and mucin 5AC (F, I) as described in materials and methods. The original magnification of the images was ×100 (A, B) and ×200 (C, D, E, F, G, H, I). |
PMC1459147_F3_5482.jpg | What is being portrayed in this visual content? | WFDC2 is abnormally expressed in the Cystic Fibrosis lung. Sections of normal (A, B) and Cystic Fibrosis lung (C – I) were stained with WFDC2 (A, C, D, G), SLPI (B, E, H) and mucin 5AC (F, I) as described in materials and methods. The original magnification of the images was ×100 (A, B) and ×200 (C, D, E, F, G, H, I). |
PMC1459147_F5_5491.jpg | What stands out most in this visual? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5495.jpg | What stands out most in this visual? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5489.jpg | What is the main focus of this visual representation? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5487.jpg | What can you see in this picture? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5488.jpg | What does this image primarily show? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5494.jpg | What is the central feature of this picture? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5490.jpg | What can you see in this picture? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459147_F5_5493.jpg | What can you see in this picture? | Distribution of WFDC2 in Pulmonary Neoplasms. Immunohistochemistry for WFDC2 was performed on three commercial lung cancer tissue arrays as described in materials and methods. Representative samples were chosen for imaging. Examples of focal (A, B) and positive staining (C) in adenocarcinomas, positive staining in a BAC (D) and focal staining in a case of squamous carcinoma (E) are shown as well as a negative case of squamous carcinoma (F), focal staining in a case of adenoid cystic carcinoma (G) and a negative case of squamous carcinoma (H) and a mesothelioma (I). The original magnification of the images was 200X. |
PMC1459153_F1_5507.jpg | What is the dominant medical problem in this image? | Comparison of normal and DSLD-affected tendons. A. Only thin septa (*) separate bundles of collagen and elastic fibers in a normal tendon. Hematoxylin & eosin, magnification × 200. B. A section of DSLD-affected tendon reveals PG deposits (*) between collagen fibers and in septa. Hematoxylin & eosin, magnification × 200. C. A section of tendon from a horse without DSLD shows the presence of fibrosis or scar tissue (*) between collagen fibers and in septa. Hematoxylin & eosin, magnification × 200. D. A proliferative lesion found in one DSLD case consists of swirls of active fibroblasts in young, well vascularized tissue. Hematoxylin & eosin, magnification × 200. |
PMC1459153_F1_5508.jpg | Can you identify the primary element in this image? | Comparison of normal and DSLD-affected tendons. A. Only thin septa (*) separate bundles of collagen and elastic fibers in a normal tendon. Hematoxylin & eosin, magnification × 200. B. A section of DSLD-affected tendon reveals PG deposits (*) between collagen fibers and in septa. Hematoxylin & eosin, magnification × 200. C. A section of tendon from a horse without DSLD shows the presence of fibrosis or scar tissue (*) between collagen fibers and in septa. Hematoxylin & eosin, magnification × 200. D. A proliferative lesion found in one DSLD case consists of swirls of active fibroblasts in young, well vascularized tissue. Hematoxylin & eosin, magnification × 200. |
PMC1459153_F2_5497.jpg | What can you see in this picture? | Immunostaining of metaplastic cartilage. A. Immunostaining reveals very little decorin, magnification × 500. B. The cartilage reveals the presence of aggrecan (▶) in the cytoplasm, magnification × 400. C. The same chondrocytes were also positive for biglycan (▶), magnification × 400. Countestain: hematoxylin. |
PMC1459153_F2_5498.jpg | What is the focal point of this photograph? | Immunostaining of metaplastic cartilage. A. Immunostaining reveals very little decorin, magnification × 500. B. The cartilage reveals the presence of aggrecan (▶) in the cytoplasm, magnification × 400. C. The same chondrocytes were also positive for biglycan (▶), magnification × 400. Countestain: hematoxylin. |
PMC1459153_F2_5496.jpg | What key item or scene is captured in this photo? | Immunostaining of metaplastic cartilage. A. Immunostaining reveals very little decorin, magnification × 500. B. The cartilage reveals the presence of aggrecan (▶) in the cytoplasm, magnification × 400. C. The same chondrocytes were also positive for biglycan (▶), magnification × 400. Countestain: hematoxylin. |
PMC1459153_F3_5500.jpg | Can you identify the primary element in this image? | Histopathological changes in nuchal ligament. A. Only thin septa separate bundles of collagen and elastic fibers in a normal nuchal ligament. Hematoxylin & eosin, magnification × 200 (× 200). B. In DSLD – affected tissue streaks of proteoglycans (▶) separate bundles of collagen and elastic fibers. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains very lightly normal nuchal ligament, magnification × 200. D. PGs accumulated among bundles stain intensively with alcian blue (▶) in DSLD-affected nuchal ligament. |
PMC1459153_F3_5501.jpg | What does this image primarily show? | Histopathological changes in nuchal ligament. A. Only thin septa separate bundles of collagen and elastic fibers in a normal nuchal ligament. Hematoxylin & eosin, magnification × 200 (× 200). B. In DSLD – affected tissue streaks of proteoglycans (▶) separate bundles of collagen and elastic fibers. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains very lightly normal nuchal ligament, magnification × 200. D. PGs accumulated among bundles stain intensively with alcian blue (▶) in DSLD-affected nuchal ligament. |
PMC1459153_F3_5499.jpg | What does this image primarily show? | Histopathological changes in nuchal ligament. A. Only thin septa separate bundles of collagen and elastic fibers in a normal nuchal ligament. Hematoxylin & eosin, magnification × 200 (× 200). B. In DSLD – affected tissue streaks of proteoglycans (▶) separate bundles of collagen and elastic fibers. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains very lightly normal nuchal ligament, magnification × 200. D. PGs accumulated among bundles stain intensively with alcian blue (▶) in DSLD-affected nuchal ligament. |
PMC1459153_F4_5504.jpg | What object or scene is depicted here? | Electron micrographs of normal and DSLD-affected tendon. A. A cross-section of normal tendon reveals that most collagen fibrils have fairly large diameters. B. A marked increase in small collagen fibrils was observed in cross-sections of DSLD-affected tendon. |
PMC1459153_F4_5503.jpg | What is the dominant medical problem in this image? | Electron micrographs of normal and DSLD-affected tendon. A. A cross-section of normal tendon reveals that most collagen fibrils have fairly large diameters. B. A marked increase in small collagen fibrils was observed in cross-sections of DSLD-affected tendon. |
PMC1459153_F7_5511.jpg | What key item or scene is captured in this photo? | Histopathological changes in arteries. A. The media of normal aortic arch shows orderly aligned elastic fibers and very little PGs between them. Hematoxylin & eosin, magnification × 200. B. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected aortic arch. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains material aligned closely with elastic fibers in normal arch, magnification × 200. D. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected arch, magnification × 200. E. The media of normal coronary artery shows orderly aligned elastic fibers separated by thin layers of PGs (▶) between them. Hematoxylin & eosin, magnification × 200 (× 200). F. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected coronary artery. Hematoxylin & eosin, magnification × 200. G. Alcian blue stains material aligned closely with elastic fibers in the media of normal coronary artery, magnification × 100. H. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected coronary artery, magnification × 100. |
PMC1459153_F7_5514.jpg | What is the dominant medical problem in this image? | Histopathological changes in arteries. A. The media of normal aortic arch shows orderly aligned elastic fibers and very little PGs between them. Hematoxylin & eosin, magnification × 200. B. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected aortic arch. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains material aligned closely with elastic fibers in normal arch, magnification × 200. D. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected arch, magnification × 200. E. The media of normal coronary artery shows orderly aligned elastic fibers separated by thin layers of PGs (▶) between them. Hematoxylin & eosin, magnification × 200 (× 200). F. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected coronary artery. Hematoxylin & eosin, magnification × 200. G. Alcian blue stains material aligned closely with elastic fibers in the media of normal coronary artery, magnification × 100. H. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected coronary artery, magnification × 100. |
PMC1459153_F7_5515.jpg | Describe the main subject of this image. | Histopathological changes in arteries. A. The media of normal aortic arch shows orderly aligned elastic fibers and very little PGs between them. Hematoxylin & eosin, magnification × 200. B. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected aortic arch. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains material aligned closely with elastic fibers in normal arch, magnification × 200. D. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected arch, magnification × 200. E. The media of normal coronary artery shows orderly aligned elastic fibers separated by thin layers of PGs (▶) between them. Hematoxylin & eosin, magnification × 200 (× 200). F. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected coronary artery. Hematoxylin & eosin, magnification × 200. G. Alcian blue stains material aligned closely with elastic fibers in the media of normal coronary artery, magnification × 100. H. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected coronary artery, magnification × 100. |
PMC1459153_F7_5512.jpg | What stands out most in this visual? | Histopathological changes in arteries. A. The media of normal aortic arch shows orderly aligned elastic fibers and very little PGs between them. Hematoxylin & eosin, magnification × 200. B. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected aortic arch. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains material aligned closely with elastic fibers in normal arch, magnification × 200. D. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected arch, magnification × 200. E. The media of normal coronary artery shows orderly aligned elastic fibers separated by thin layers of PGs (▶) between them. Hematoxylin & eosin, magnification × 200 (× 200). F. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected coronary artery. Hematoxylin & eosin, magnification × 200. G. Alcian blue stains material aligned closely with elastic fibers in the media of normal coronary artery, magnification × 100. H. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected coronary artery, magnification × 100. |
PMC1459153_F7_5509.jpg | Describe the main subject of this image. | Histopathological changes in arteries. A. The media of normal aortic arch shows orderly aligned elastic fibers and very little PGs between them. Hematoxylin & eosin, magnification × 200. B. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected aortic arch. Hematoxylin & eosin, magnification × 200. C. Alcian blue stains material aligned closely with elastic fibers in normal arch, magnification × 200. D. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected arch, magnification × 200. E. The media of normal coronary artery shows orderly aligned elastic fibers separated by thin layers of PGs (▶) between them. Hematoxylin & eosin, magnification × 200 (× 200). F. Small pools of PGs (▶) separate fibers and cells in the media of DSLD-affected coronary artery. Hematoxylin & eosin, magnification × 200. G. Alcian blue stains material aligned closely with elastic fibers in the media of normal coronary artery, magnification × 100. H. Pools of PGs (▶) stain strongly with alcian blue in the media of DSLD-affected coronary artery, magnification × 100. |
PMC1459158_F1_5520.jpg | What's the most prominent thing you notice in this picture? | Chest radiograph showing a) left hemothorax and enlarged cardiac silhouette b) residual hemothorax and persistent enlarged cardiac silhouette after pleural tube drainage. |
PMC1459158_F1_5519.jpg | Describe the main subject of this image. | Chest radiograph showing a) left hemothorax and enlarged cardiac silhouette b) residual hemothorax and persistent enlarged cardiac silhouette after pleural tube drainage. |
PMC1459158_F2_5518.jpg | What is the core subject represented in this visual? | CT Scan of chest showing a) hemopericardium b) hemopericardium and residual left pleural clot and blood despite pleural drainage. |
PMC1459158_F2_5517.jpg | What is the central feature of this picture? | CT Scan of chest showing a) hemopericardium b) hemopericardium and residual left pleural clot and blood despite pleural drainage. |
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