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PMC1459159_F2_5521.jpg | What is the main focus of this visual representation? | Preoperative computed tomographic scan demonstrated a tumor mass located in the mediastinum next to descending aorta and associated with pleural effusion. |
PMC1459166_F4_5524.jpg | What is the principal component of this image? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5522.jpg | What is the main focus of this visual representation? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5528.jpg | What is the principal component of this image? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5530.jpg | What object or scene is depicted here? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5529.jpg | What is the core subject represented in this visual? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5531.jpg | What is the core subject represented in this visual? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5525.jpg | What is being portrayed in this visual content? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5526.jpg | What is the core subject represented in this visual? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459166_F4_5523.jpg | What does this image primarily show? | In situ localization of CART mRNA within granulosa cells of dominant ovulatory versus nonovulatory (subordinate) bovine follicles collected before (0 h) and after (12 h) GnRH injection to induce the preovulatory gonadotropin surge (n = 3 each). A) Bright field micrograph of section through dominant ovulatory follicle (denoted as DF) and adjacent non-ovulatory subordinate follicle (denoted as SF) collected before the preovulatory gonadotropin surge (0 h timepoint) and hybridized with 35S-antisense CART cRNA. B) Bright field micrograph of section through DF and adjacent SF collected after the preovulatory gonadotropin surge (12 h timepoint) and hybridized with 35S-antisense CART cRNA. C) Dark field micrograph of section adjacent to one depicted in Panel A. D) Dark field micrograph of section adjacent to one depicted in Panel B. Note localization of CART mRNA to GC of the SF (right) but not the DF (left) depicted in Panels A-D. E) Magnified bright field micrograph of SF depicted in Panels A and C. F) Magnified bright field micrograph of SF depicted in Panels B and D. G) Magnified dark field micrograph of Panel E. H) Magnified dark field micrograph of Panel F. Note localization of CART mRNA specifically to GC of SF in panels G and H. I and J) Dark field micrographs of sections adjacent to ones depicted in Panels E and F hybridized with 35S-sense CART cRNA (negative control). A-D: magnification = 100X, scale bar = 200 μm; E-J: magnification = 400X, scale bar = 50 μm. Granulosa cell layer denoted as GC. Theca cell layer denoted as TC. Reproduced from Kobayashi et al., 2004 [59]. |
PMC1459174_F1_5532.jpg | What can you see in this picture? | Pre-operative barium meal showing a capacious residual stomach with efficient emptying function through a wide gastrojejunostomy. |
PMC1459201_F3_5533.jpg | Describe the main subject of this image. | A: The echocontrast appearance in hypertrophic muscle segment, B: No angiographic appearance after alcohol injection in first septal branch (Black arrow), C: Post-procedural continuous-wave Doppler recordings. |
PMC1459201_F3_5534.jpg | What is the main focus of this visual representation? | A: The echocontrast appearance in hypertrophic muscle segment, B: No angiographic appearance after alcohol injection in first septal branch (Black arrow), C: Post-procedural continuous-wave Doppler recordings. |
PMC1459268_F1_5537.jpg | What is the focal point of this photograph? | Photograph showing burst abdomen with fecal fistula in a patient of typhoid perforation following laparotomy |
PMC1459872_F5_5539.jpg | What is being portrayed in this visual content? | Neoplastic transformation and invasion of isografts of Comma/PDK1 cells. H&E stained paraffin-embedded tissue obtained from isografts of cells transduced with either empty virus (Ctl) or PDK1 (T1, T2, T3) 8 weeks after transplantation. Mammary isografts of control cells have normal lobuloalveolar ductal morphology with surrounding adipose tissue. Comma/PDK1 isografts produced poorly differentiated adenocarcinomas with solid cords of cells with little gland formation (T1), invasion of underlying skeletal muscle (T2) and vascularity (T3). Magnification: 100×. |
PMC1459872_F5_5538.jpg | What is the main focus of this visual representation? | Neoplastic transformation and invasion of isografts of Comma/PDK1 cells. H&E stained paraffin-embedded tissue obtained from isografts of cells transduced with either empty virus (Ctl) or PDK1 (T1, T2, T3) 8 weeks after transplantation. Mammary isografts of control cells have normal lobuloalveolar ductal morphology with surrounding adipose tissue. Comma/PDK1 isografts produced poorly differentiated adenocarcinomas with solid cords of cells with little gland formation (T1), invasion of underlying skeletal muscle (T2) and vascularity (T3). Magnification: 100×. |
PMC1459932_f1-ehp0114-000759_5546.jpg | Can you identify the primary element in this image? | Chest radiograph (A) and HRCT images (B–D) of patient’s lungs. (A) Frontal chest radiograph showing mild symmetric linear and reticular opacities (arrows) in the upper lobes bilaterally; these opacities are associated with bilateral hilar prominence, suggesting lymphadenopathy. Note the upper lobe distribution of the findings as well as the absence of associated pleural thickening. (B) Axial HRCT image through the lung apices shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Nodular interlobular septal thickening is also present (arrowheads). (C) Axial HRCT image through the upper lungs slightly caudal to (B) shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Mild interlobular septal thickening is again present (arrowheads). Note the posterior distribution of abnormalities. D) Contrast-enhanced axial CT image shows subcarinal (*) and mild bilateral hilar (arrows) lymphadenopathy. |
PMC1459932_f1-ehp0114-000759_5545.jpg | What is the central feature of this picture? | Chest radiograph (A) and HRCT images (B–D) of patient’s lungs. (A) Frontal chest radiograph showing mild symmetric linear and reticular opacities (arrows) in the upper lobes bilaterally; these opacities are associated with bilateral hilar prominence, suggesting lymphadenopathy. Note the upper lobe distribution of the findings as well as the absence of associated pleural thickening. (B) Axial HRCT image through the lung apices shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Nodular interlobular septal thickening is also present (arrowheads). (C) Axial HRCT image through the upper lungs slightly caudal to (B) shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Mild interlobular septal thickening is again present (arrowheads). Note the posterior distribution of abnormalities. D) Contrast-enhanced axial CT image shows subcarinal (*) and mild bilateral hilar (arrows) lymphadenopathy. |
PMC1459932_f1-ehp0114-000759_5544.jpg | What is the core subject represented in this visual? | Chest radiograph (A) and HRCT images (B–D) of patient’s lungs. (A) Frontal chest radiograph showing mild symmetric linear and reticular opacities (arrows) in the upper lobes bilaterally; these opacities are associated with bilateral hilar prominence, suggesting lymphadenopathy. Note the upper lobe distribution of the findings as well as the absence of associated pleural thickening. (B) Axial HRCT image through the lung apices shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Nodular interlobular septal thickening is also present (arrowheads). (C) Axial HRCT image through the upper lungs slightly caudal to (B) shows bilateral, patchy bronchovascular thickening with a nodular appearance (small double arrows). Mild interlobular septal thickening is again present (arrowheads). Note the posterior distribution of abnormalities. D) Contrast-enhanced axial CT image shows subcarinal (*) and mild bilateral hilar (arrows) lymphadenopathy. |
PMC1459932_f3-ehp0114-000759_5541.jpg | What's the most prominent thing you notice in this picture? | Histopathologic features of transbronchial biopsies. (A) Anthracotic pigment accumulating along alveolar septae (arrowheads) and within a pigmented dust macule (single arrow); bar = 200 μm. (B) A high-power photomicrograph containing a mixture of fibroblasts and carbon-laden macrophages; bar = 50 μm. (C) A fibrotic scar is seen projecting into surrounding interstitial septae giving a stellate appearance (arrowheads); bar = 100 μm. |
PMC1459932_f3-ehp0114-000759_5543.jpg | What is the central feature of this picture? | Histopathologic features of transbronchial biopsies. (A) Anthracotic pigment accumulating along alveolar septae (arrowheads) and within a pigmented dust macule (single arrow); bar = 200 μm. (B) A high-power photomicrograph containing a mixture of fibroblasts and carbon-laden macrophages; bar = 50 μm. (C) A fibrotic scar is seen projecting into surrounding interstitial septae giving a stellate appearance (arrowheads); bar = 100 μm. |
PMC1463003_F3_5549.jpg | What object or scene is depicted here? | Tumor slices stained with hematoxylin and eosin. Subcutaneous tumors were excised from BALB/c nude mice with different treatments under sodium pentobarbital (50 mg/kg, i.p.) anesthesia. Tumors were rinsed twice in normal saline (NS) then fixed in 4% polyparaformaldehyde for 12 hours, embedded in paraffin, sliced and sections stained with hematoxylin and eosin for light microscopic observation (× 400 magnification). A: DMEM group; B: GCV+Dox group; C: GCV+Virus group; D: Dox+GCV+Virus group. |
PMC1463003_F3_5550.jpg | What is being portrayed in this visual content? | Tumor slices stained with hematoxylin and eosin. Subcutaneous tumors were excised from BALB/c nude mice with different treatments under sodium pentobarbital (50 mg/kg, i.p.) anesthesia. Tumors were rinsed twice in normal saline (NS) then fixed in 4% polyparaformaldehyde for 12 hours, embedded in paraffin, sliced and sections stained with hematoxylin and eosin for light microscopic observation (× 400 magnification). A: DMEM group; B: GCV+Dox group; C: GCV+Virus group; D: Dox+GCV+Virus group. |
PMC1463003_F3_5548.jpg | What is shown in this image? | Tumor slices stained with hematoxylin and eosin. Subcutaneous tumors were excised from BALB/c nude mice with different treatments under sodium pentobarbital (50 mg/kg, i.p.) anesthesia. Tumors were rinsed twice in normal saline (NS) then fixed in 4% polyparaformaldehyde for 12 hours, embedded in paraffin, sliced and sections stained with hematoxylin and eosin for light microscopic observation (× 400 magnification). A: DMEM group; B: GCV+Dox group; C: GCV+Virus group; D: Dox+GCV+Virus group. |
PMC1463015_ppat-0020046-g007_5556.jpg | What is being portrayed in this visual content? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5560.jpg | What object or scene is depicted here? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5555.jpg | What is shown in this image? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5553.jpg | What is being portrayed in this visual content? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5554.jpg | What object or scene is depicted here? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5557.jpg | What is the dominant medical problem in this image? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5559.jpg | What's the most prominent thing you notice in this picture? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463015_ppat-0020046-g007_5562.jpg | What is the central feature of this picture? | PI(4)P Is a Lipid Marker of LCVs Harboring Icm/Dot–Proficient L. pneumophila
(A, B, and D) Confocal micrographs of LCVs in lysates of (A) calnexin-GFP–labeled Dictyostelium, (B) VatM-GFP–labeled Dictyostelium, or (D) RAW264.7 macrophages infected with DsRed-Express–labeled L. pneumophila are shown. The lysates were prepared with a ball homogenizer, and PI(4)P was visualized on the LCVs using as probes either the PH domain of the PI(4)P-binding protein FAPP1 fused to GST, an antibody against PI(4)P, or GST-SidC. Using GST alone or omission of the anti-PI(4)P antibody did not label the LCVs. Bar denotes 2 μm (magnification of all images is identical).(C) Quantification of PI(4)P-positive calnexin-GFP–labeled (n = 300) or VatM-GFP–labeled (n = 100) LCVs in Dictyostelium wild-type strain Ax3. |
PMC1463044_pgen-0020071-g002_5568.jpg | What is shown in this image? |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5569.jpg | Describe the main subject of this image. |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5566.jpg | What is shown in this image? |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5567.jpg | What object or scene is depicted here? |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5570.jpg | What's the most prominent thing you notice in this picture? |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5565.jpg | Describe the main subject of this image. |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1463044_pgen-0020071-g002_5563.jpg | What is the focal point of this photograph? |
Drosophila Nbs Is Required for Damage-Induced Cell Cycle ArrestThird-instar larval wing discs were mock treated or treated with various doses (250, 500, 1,000, and 4,000 rads) of X-rays and then stained with an antibody against phosphorylated histone H3.(A–I) The pattern of mitotic cells in untreated and irradiated wild-type (A–C), nbs mutant (D–F), and tefu mutant (G–I) larval wing discs are shown. At 1,000 and 4,000 rads, mitosis is blocked in wild-type wing discs (B and C) whereas nbs mutant discs fail to arrest (E and F). tefu mutant wing discs have a partial mitotic arrest following treatment with 1,000 rads (H). At 4,000 rads, mitosis is completely blocked in tefu mutant wing discs (I).(J) The ratio of mitotic cells in wild-type, nbs, tefu, and mei-41 mutant wing discs following X-irradiation to the number of mitotic cells in untreated discs of the same genotype is shown. Error bars indicate the standard error of the mean. |
PMC1464131_F4_5574.jpg | What can you see in this picture? | Fluorescence microscopy images. a) frozen section of normal skin stained with hematoxylin viewed by light microscopy, b) auto fluorescence of untreated epidermis, c) auto fluorescence of petrolatum with penetration only into stratum corneum, d)auto fluorescence of the total lipid formulation (variation A) with penetration into the viable epidermis and dermis. |
PMC1464131_F4_5571.jpg | Describe the main subject of this image. | Fluorescence microscopy images. a) frozen section of normal skin stained with hematoxylin viewed by light microscopy, b) auto fluorescence of untreated epidermis, c) auto fluorescence of petrolatum with penetration only into stratum corneum, d)auto fluorescence of the total lipid formulation (variation A) with penetration into the viable epidermis and dermis. |
PMC1464131_F4_5573.jpg | What stands out most in this visual? | Fluorescence microscopy images. a) frozen section of normal skin stained with hematoxylin viewed by light microscopy, b) auto fluorescence of untreated epidermis, c) auto fluorescence of petrolatum with penetration only into stratum corneum, d)auto fluorescence of the total lipid formulation (variation A) with penetration into the viable epidermis and dermis. |
PMC1464131_F4_5572.jpg | Can you identify the primary element in this image? | Fluorescence microscopy images. a) frozen section of normal skin stained with hematoxylin viewed by light microscopy, b) auto fluorescence of untreated epidermis, c) auto fluorescence of petrolatum with penetration only into stratum corneum, d)auto fluorescence of the total lipid formulation (variation A) with penetration into the viable epidermis and dermis. |
PMC1464140_F1_5578.jpg | What is the central feature of this picture? | Magnetic Resonance Imaging (MRI). MRI_axial: (A) Axial T1-weighted Spin Echo (SE) MRI shows circumferential thickening of the trachea (white arrow) with intermediate signal intensity of the thickened mucosa/submucosa. (B) T2-weighted Turbo SE MRI at the same level. Note the pathological high signal intensity of the thickened mucosa/submucosa, which corresponds to mucosal inflammation. MRI_sag: (C) Sagittal T1-weighted SE MRI shows irregular narrowing of the airway (white arrow). (D) Sagittal T2-weighted Turbo SE MRI of the subglottic trachea shows thickening of the tracheal lumen with high signal intensity due to inflammation. |
PMC1464140_F1_5579.jpg | What's the most prominent thing you notice in this picture? | Magnetic Resonance Imaging (MRI). MRI_axial: (A) Axial T1-weighted Spin Echo (SE) MRI shows circumferential thickening of the trachea (white arrow) with intermediate signal intensity of the thickened mucosa/submucosa. (B) T2-weighted Turbo SE MRI at the same level. Note the pathological high signal intensity of the thickened mucosa/submucosa, which corresponds to mucosal inflammation. MRI_sag: (C) Sagittal T1-weighted SE MRI shows irregular narrowing of the airway (white arrow). (D) Sagittal T2-weighted Turbo SE MRI of the subglottic trachea shows thickening of the tracheal lumen with high signal intensity due to inflammation. |
PMC1464140_F1_5580.jpg | What stands out most in this visual? | Magnetic Resonance Imaging (MRI). MRI_axial: (A) Axial T1-weighted Spin Echo (SE) MRI shows circumferential thickening of the trachea (white arrow) with intermediate signal intensity of the thickened mucosa/submucosa. (B) T2-weighted Turbo SE MRI at the same level. Note the pathological high signal intensity of the thickened mucosa/submucosa, which corresponds to mucosal inflammation. MRI_sag: (C) Sagittal T1-weighted SE MRI shows irregular narrowing of the airway (white arrow). (D) Sagittal T2-weighted Turbo SE MRI of the subglottic trachea shows thickening of the tracheal lumen with high signal intensity due to inflammation. |
PMC1464140_F2_5576.jpg | What is the dominant medical problem in this image? | Fiberoptic bronchoscopy and histological examination of tracheal stenosis. (A) Circumferential tracheal stenosis, greater than 70%, 1–2 cm beneath the vocal cords and marked inflammation of tracheal mucosa was noted at fiberoptic bronchoscopy. (B) Histological examination revealed acute and chronic inflammation and extended ulceration of bronchial mucosa, without granulomas. |
PMC1464140_F2_5575.jpg | Can you identify the primary element in this image? | Fiberoptic bronchoscopy and histological examination of tracheal stenosis. (A) Circumferential tracheal stenosis, greater than 70%, 1–2 cm beneath the vocal cords and marked inflammation of tracheal mucosa was noted at fiberoptic bronchoscopy. (B) Histological examination revealed acute and chronic inflammation and extended ulceration of bronchial mucosa, without granulomas. |
PMC1464140_F3_5581.jpg | Describe the main subject of this image. | Computed Tomography (CT). (A) CT_ volume rendering technique (VRT): The VRT of the tracheobronchial tree, clearly demonstrates the segment of irregular stenosis of the subglottic trachea (white arrow). (B) CT _axial: CT scan shows marked circumferential narrowing of the subglottic part of the trachea. No calcification can be seen. (C and D) CT_sag_multi planar reconstruction (MPR): On (C) end-expiration and (D) end-inspiration sagittal MPR of CT scan, a dynamic collapse of the trachea can not be documented. |
PMC1464147_F3_5584.jpg | What object or scene is depicted here? | CT and MRI scans of the abdomen. In 4/2005, a mass (circle) is shown which has also been there in earlier scans but had been interpreted as normal intestine. Fat-saturated and T1-weighted MRI scanning after gadolinium shows a mass in the mesentery outside the intestine. |
PMC1464147_F3_5585.jpg | What is the focal point of this photograph? | CT and MRI scans of the abdomen. In 4/2005, a mass (circle) is shown which has also been there in earlier scans but had been interpreted as normal intestine. Fat-saturated and T1-weighted MRI scanning after gadolinium shows a mass in the mesentery outside the intestine. |
PMC1464147_F3_5588.jpg | What is being portrayed in this visual content? | CT and MRI scans of the abdomen. In 4/2005, a mass (circle) is shown which has also been there in earlier scans but had been interpreted as normal intestine. Fat-saturated and T1-weighted MRI scanning after gadolinium shows a mass in the mesentery outside the intestine. |
PMC1464147_F3_5586.jpg | What can you see in this picture? | CT and MRI scans of the abdomen. In 4/2005, a mass (circle) is shown which has also been there in earlier scans but had been interpreted as normal intestine. Fat-saturated and T1-weighted MRI scanning after gadolinium shows a mass in the mesentery outside the intestine. |
PMC1464147_F3_5587.jpg | What is the core subject represented in this visual? | CT and MRI scans of the abdomen. In 4/2005, a mass (circle) is shown which has also been there in earlier scans but had been interpreted as normal intestine. Fat-saturated and T1-weighted MRI scanning after gadolinium shows a mass in the mesentery outside the intestine. |
PMC1464149_F1_5590.jpg | What is the focal point of this photograph? | Dynamic computed tomographic scans with maximum intensity projection (MIP) images in the coronal (a) and parasagittal planes (b, c) revealing diminished caliber of the intracranial portions of the left internal carotid artery (arrow). The contralateral right internal carotid artery displays normal caliber (arrowhead). |
PMC1464149_F1_5591.jpg | What is the dominant medical problem in this image? | Dynamic computed tomographic scans with maximum intensity projection (MIP) images in the coronal (a) and parasagittal planes (b, c) revealing diminished caliber of the intracranial portions of the left internal carotid artery (arrow). The contralateral right internal carotid artery displays normal caliber (arrowhead). |
PMC1464149_F1_5592.jpg | What's the most prominent thing you notice in this picture? | Dynamic computed tomographic scans with maximum intensity projection (MIP) images in the coronal (a) and parasagittal planes (b, c) revealing diminished caliber of the intracranial portions of the left internal carotid artery (arrow). The contralateral right internal carotid artery displays normal caliber (arrowhead). |
PMC1468401_F2_5593.jpg | What is the central feature of this picture? | Computed tomography showing a low density lesion with irregular and thick margins in lateral neck region. |
PMC1471777_F1_5597.jpg | What can you see in this picture? | Left: Coronal computed tomography image shows right septal deviation (yellow arrow), left concha bullosa (thick white arrow), thickening of the mucosa covering the concha bullosa, and bilateral maxillary sinusitis (vertical black arrow). Right: Intraoperative views of the polyp in the left concha bullosa. (black arrow shows polyp) |
PMC1471777_F1_5598.jpg | Can you identify the primary element in this image? | Left: Coronal computed tomography image shows right septal deviation (yellow arrow), left concha bullosa (thick white arrow), thickening of the mucosa covering the concha bullosa, and bilateral maxillary sinusitis (vertical black arrow). Right: Intraoperative views of the polyp in the left concha bullosa. (black arrow shows polyp) |
PMC1475579_F4_5600.jpg | What can you see in this picture? | Re-specification of the distribution of pIN-G by an autonomous targeting signal. HEK293 cells transiently transfected with pIN-G or pIN-TGN38 – a fusion construct corresponding to pIN-G bearing the autonomous targeting signal of the Trans-Golgi Network resident protein TGN-38 – were fixed, stained and imaged on a Delta-Vision workstation. Panel A, pIN-G; Panel B, pIN-TGN. HEK293 cells transiently transfected with pIN-TGN38, were fixed, permeabilised and treated with antibodies (See Methods) to the following organelle markers: C, GM130 (Golgi apparatus); D, EEA1 (early endosomes); E, Calnexin (endoplasmic reticulum) and F, lamp-1 (Lysosomes) followed by the appropriate Cy-3 conjugated secondary antibody. Green denotes pIN fluorescence while red corresponds to the organelle marker. Areas of red/green overlap are shown in yellow. Blue indicates DAPI-stained nuclei. Scale Bar: 15 μM. |
PMC1475579_F4_5601.jpg | What stands out most in this visual? | Re-specification of the distribution of pIN-G by an autonomous targeting signal. HEK293 cells transiently transfected with pIN-G or pIN-TGN38 – a fusion construct corresponding to pIN-G bearing the autonomous targeting signal of the Trans-Golgi Network resident protein TGN-38 – were fixed, stained and imaged on a Delta-Vision workstation. Panel A, pIN-G; Panel B, pIN-TGN. HEK293 cells transiently transfected with pIN-TGN38, were fixed, permeabilised and treated with antibodies (See Methods) to the following organelle markers: C, GM130 (Golgi apparatus); D, EEA1 (early endosomes); E, Calnexin (endoplasmic reticulum) and F, lamp-1 (Lysosomes) followed by the appropriate Cy-3 conjugated secondary antibody. Green denotes pIN fluorescence while red corresponds to the organelle marker. Areas of red/green overlap are shown in yellow. Blue indicates DAPI-stained nuclei. Scale Bar: 15 μM. |
PMC1475579_F4_5603.jpg | Describe the main subject of this image. | Re-specification of the distribution of pIN-G by an autonomous targeting signal. HEK293 cells transiently transfected with pIN-G or pIN-TGN38 – a fusion construct corresponding to pIN-G bearing the autonomous targeting signal of the Trans-Golgi Network resident protein TGN-38 – were fixed, stained and imaged on a Delta-Vision workstation. Panel A, pIN-G; Panel B, pIN-TGN. HEK293 cells transiently transfected with pIN-TGN38, were fixed, permeabilised and treated with antibodies (See Methods) to the following organelle markers: C, GM130 (Golgi apparatus); D, EEA1 (early endosomes); E, Calnexin (endoplasmic reticulum) and F, lamp-1 (Lysosomes) followed by the appropriate Cy-3 conjugated secondary antibody. Green denotes pIN fluorescence while red corresponds to the organelle marker. Areas of red/green overlap are shown in yellow. Blue indicates DAPI-stained nuclei. Scale Bar: 15 μM. |
PMC1475579_F4_5602.jpg | What key item or scene is captured in this photo? | Re-specification of the distribution of pIN-G by an autonomous targeting signal. HEK293 cells transiently transfected with pIN-G or pIN-TGN38 – a fusion construct corresponding to pIN-G bearing the autonomous targeting signal of the Trans-Golgi Network resident protein TGN-38 – were fixed, stained and imaged on a Delta-Vision workstation. Panel A, pIN-G; Panel B, pIN-TGN. HEK293 cells transiently transfected with pIN-TGN38, were fixed, permeabilised and treated with antibodies (See Methods) to the following organelle markers: C, GM130 (Golgi apparatus); D, EEA1 (early endosomes); E, Calnexin (endoplasmic reticulum) and F, lamp-1 (Lysosomes) followed by the appropriate Cy-3 conjugated secondary antibody. Green denotes pIN fluorescence while red corresponds to the organelle marker. Areas of red/green overlap are shown in yellow. Blue indicates DAPI-stained nuclei. Scale Bar: 15 μM. |
PMC1475638_F6_5607.jpg | What can you see in this picture? | mRNA expression of surfactant protein D, determined with in situ hybridization on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development at birth (A), day 6 (C), 10 (E) and 14 (B) and after exposure to hyperoxia on day 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F6_5608.jpg | What's the most prominent thing you notice in this picture? | mRNA expression of surfactant protein D, determined with in situ hybridization on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development at birth (A), day 6 (C), 10 (E) and 14 (B) and after exposure to hyperoxia on day 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F6_5609.jpg | What is the core subject represented in this visual? | mRNA expression of surfactant protein D, determined with in situ hybridization on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development at birth (A), day 6 (C), 10 (E) and 14 (B) and after exposure to hyperoxia on day 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F8_5612.jpg | What does this image primarily show? | Immunohistochemical staining of surfactant protein A on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development on day 3 (A), day 6 (C) and day 10 (E) and after exposure to hyperoxia on day 3 (B), 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F8_5616.jpg | Can you identify the primary element in this image? | Immunohistochemical staining of surfactant protein A on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development on day 3 (A), day 6 (C) and day 10 (E) and after exposure to hyperoxia on day 3 (B), 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F8_5615.jpg | Describe the main subject of this image. | Immunohistochemical staining of surfactant protein A on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development on day 3 (A), day 6 (C) and day 10 (E) and after exposure to hyperoxia on day 3 (B), 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475638_F8_5614.jpg | What is the focal point of this photograph? | Immunohistochemical staining of surfactant protein A on formaldehyde fixed paraffin sections of rat lungs during normal postnatal development on day 3 (A), day 6 (C) and day 10 (E) and after exposure to hyperoxia on day 3 (B), 6 (D) and 10 (F) at a 200-fold magnification. a = alveolus, br = bronchus, bv = blood vessel, s = saccule, tb = terminal bronchus. |
PMC1475641_F7_5619.jpg | What is the principal component of this image? | Gecko crystallin contact site prediction. 15 of the 25 sites experiencing amino acid replacement along the branch leading to the gecko crystallin protein (Figure 6; branch 7) are highlighted in red. The reference structure is PDB:1gglA. (A-D) Successive quarter turns of the molecule about its y-axis. |
PMC1475641_F7_5618.jpg | What is being portrayed in this visual content? | Gecko crystallin contact site prediction. 15 of the 25 sites experiencing amino acid replacement along the branch leading to the gecko crystallin protein (Figure 6; branch 7) are highlighted in red. The reference structure is PDB:1gglA. (A-D) Successive quarter turns of the molecule about its y-axis. |
PMC1475641_F7_5620.jpg | Can you identify the primary element in this image? | Gecko crystallin contact site prediction. 15 of the 25 sites experiencing amino acid replacement along the branch leading to the gecko crystallin protein (Figure 6; branch 7) are highlighted in red. The reference structure is PDB:1gglA. (A-D) Successive quarter turns of the molecule about its y-axis. |
PMC1475641_F7_5617.jpg | What is being portrayed in this visual content? | Gecko crystallin contact site prediction. 15 of the 25 sites experiencing amino acid replacement along the branch leading to the gecko crystallin protein (Figure 6; branch 7) are highlighted in red. The reference structure is PDB:1gglA. (A-D) Successive quarter turns of the molecule about its y-axis. |
PMC1475834_F5_5621.jpg | What is the core subject represented in this visual? | Traffic accident with a liver rupture and leakage from a small bile duct. The DIC-CT examination led the surgeon correctly to the leaking bile duct (arrow). The diameter of the ruptured bile duct was 1 mm. |
PMC1475844_F1_5622.jpg | Describe the main subject of this image. | Photograph at initial examination showing an oversized pigmented soft mass (melanoma) arising from the palate. |
PMC1475844_F3_5624.jpg | What does this image primarily show? | A computer tomography with contrast revealed a large palate's mass, which is seemed to infiltrate the left medial pterygoid muscle. |
PMC1475844_F3_5623.jpg | What is being portrayed in this visual content? | A computer tomography with contrast revealed a large palate's mass, which is seemed to infiltrate the left medial pterygoid muscle. |
PMC1475855_F5_5626.jpg | What is the focal point of this photograph? | Arabidopsis line expressing Pt-GFP. A: Confocal image of Arabidopsis guard cells expressing Pt-GFP in the cytoplasm. The red fluorescence is chlorophyll autofluorescence from chloroplasts. B: Confocal optical sections and C: corresponding bright-field image of a root segment. Excitation with Argon laser line 476 nm; emission at 500–540 nm (green channel) and 600–660 nm (red channel); Leica TCS SP confocal laser scanning system; HC PL APD objective (40× oil). |
PMC1475855_F5_5627.jpg | What is shown in this image? | Arabidopsis line expressing Pt-GFP. A: Confocal image of Arabidopsis guard cells expressing Pt-GFP in the cytoplasm. The red fluorescence is chlorophyll autofluorescence from chloroplasts. B: Confocal optical sections and C: corresponding bright-field image of a root segment. Excitation with Argon laser line 476 nm; emission at 500–540 nm (green channel) and 600–660 nm (red channel); Leica TCS SP confocal laser scanning system; HC PL APD objective (40× oil). |
PMC1475855_F5_5625.jpg | What is the main focus of this visual representation? | Arabidopsis line expressing Pt-GFP. A: Confocal image of Arabidopsis guard cells expressing Pt-GFP in the cytoplasm. The red fluorescence is chlorophyll autofluorescence from chloroplasts. B: Confocal optical sections and C: corresponding bright-field image of a root segment. Excitation with Argon laser line 476 nm; emission at 500–540 nm (green channel) and 600–660 nm (red channel); Leica TCS SP confocal laser scanning system; HC PL APD objective (40× oil). |
PMC1475856_F3_5628.jpg | What is the central feature of this picture? | 3-D reconstruction of postoperative CT scan showing the extra-anatomic bypass graft. |
PMC1475867_F2_5638.jpg | What is the main focus of this visual representation? | Immunohistochemical staining of Syndecan 3 in uterine tissue at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) women in normal labour (group 3), (D) in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200. Positive staining is brown. The grading of staining is presented in Table 2. |
PMC1475867_F2_5640.jpg | What stands out most in this visual? | Immunohistochemical staining of Syndecan 3 in uterine tissue at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) women in normal labour (group 3), (D) in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200. Positive staining is brown. The grading of staining is presented in Table 2. |
PMC1475867_F2_5635.jpg | Describe the main subject of this image. | Immunohistochemical staining of Syndecan 3 in uterine tissue at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) women in normal labour (group 3), (D) in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200. Positive staining is brown. The grading of staining is presented in Table 2. |
PMC1475867_F2_5637.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemical staining of Syndecan 3 in uterine tissue at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) women in normal labour (group 3), (D) in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200. Positive staining is brown. The grading of staining is presented in Table 2. |
PMC1475867_F2_5639.jpg | What key item or scene is captured in this photo? | Immunohistochemical staining of Syndecan 3 in uterine tissue at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) women in normal labour (group 3), (D) in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200. Positive staining is brown. The grading of staining is presented in Table 2. |
PMC1475867_F3_5633.jpg | What's the most prominent thing you notice in this picture? | Immunohistochemical staining of Connexin 43 at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) in normal labour (group 3), D in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200 Positive staining is brown. The grading of staining is presented in Table 3. |
PMC1475867_F3_5631.jpg | What can you see in this picture? | Immunohistochemical staining of Connexin 43 at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) in normal labour (group 3), D in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200 Positive staining is brown. The grading of staining is presented in Table 3. |
PMC1475867_F3_5634.jpg | What is the dominant medical problem in this image? | Immunohistochemical staining of Connexin 43 at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) in normal labour (group 3), D in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200 Positive staining is brown. The grading of staining is presented in Table 3. |
PMC1475867_F3_5632.jpg | What is the central feature of this picture? | Immunohistochemical staining of Connexin 43 at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) in normal labour (group 3), D in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200 Positive staining is brown. The grading of staining is presented in Table 3. |
PMC1475867_F3_5630.jpg | What is the focal point of this photograph? | Immunohistochemical staining of Connexin 43 at different stages of pregnancy. (A) Non-pregnant (group 1), (B) term-pregnant (group 2), (C) in normal labour (group 3), D in prolonged labour (group 4), (E) in normal labour, magnification 1 × 400 with oil, (F) negative control. Magnification in A, B, C, D and F is 1 × 200 Positive staining is brown. The grading of staining is presented in Table 3. |
PMC1475867_F4_5647.jpg | What key item or scene is captured in this photo? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5646.jpg | What is the focal point of this photograph? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5644.jpg | What object or scene is depicted here? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5641.jpg | What is the central feature of this picture? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5643.jpg | Can you identify the primary element in this image? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5642.jpg | What is the main focus of this visual representation? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
PMC1475867_F4_5649.jpg | What stands out most in this visual? | Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I). |
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