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PMC1475867_F4_5645.jpg
Describe the main subject of this image.
Co-localization of Connexin 43 and Syndecan 3 in uterine tissue during normal labour. Sections from uterine tissue from women in normal labour (panel A-C), patients at term (panel D-F) and patients with prolonged labour (panel G-I) were obtained and prepared as described above. A monoclonal antibody against syndecan 3 was added, followed by Alexa Fluor 633 goat anti-rabbit IgG antibody. After that, a Connexin 43 antibody followed by Alexa Fluor 488 rabbit anti-mouse IgG antibody was added. The excitation for Connexin 43 (panel A, D and G) and Syndecan 3 (panel B, E and H) are shown. The merged picture demonstrates the co-localization in the tissue (panel C, F and I).
PMC1475874_F1_5650.jpg
What object or scene is depicted here?
Case 1 CT Scan. CT scan of upper abdomen in arterial phase shows low density soft tissue in region of pancreatic head and posterior to body of pancreas, and biliary stent
PMC1475874_F2_5652.jpg
What is the main focus of this visual representation?
Case 2 MRI. MRI (magnetic resonance imaging) shows mass arising from head of pancreas, as well as hepatic and renal cysts.
PMC1475874_F3_5651.jpg
What is the dominant medical problem in this image?
Case 2 Biopsy. Histological section shows large mononuclear cells, consistent with diffuse large B-cell lymphoma (H&E stain, ×200)
PMC1475874_F4_5653.jpg
What key item or scene is captured in this photo?
Case 2 Biopsy CD20 stain. CD20 positive staining of cells, consistent with B cell origin (×200)
PMC1475877_F1_5657.jpg
What is being portrayed in this visual content?
A, Positive staining for CD105 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). B, Negative staining for CD105 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). C, Negative staining for CD105 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). D, Positive staining for CD34 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). E, Positive staining for CD34 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). F, Positive staining for CD34 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200).
PMC1475877_F1_5659.jpg
What stands out most in this visual?
A, Positive staining for CD105 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). B, Negative staining for CD105 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). C, Negative staining for CD105 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). D, Positive staining for CD34 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). E, Positive staining for CD34 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). F, Positive staining for CD34 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200).
PMC1475877_F1_5658.jpg
What is shown in this image?
A, Positive staining for CD105 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). B, Negative staining for CD105 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). C, Negative staining for CD105 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). D, Positive staining for CD34 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). E, Positive staining for CD34 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). F, Positive staining for CD34 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200).
PMC1475877_F1_5654.jpg
What is the core subject represented in this visual?
A, Positive staining for CD105 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). B, Negative staining for CD105 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). C, Negative staining for CD105 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). D, Positive staining for CD34 in HCC tissue (avidin-biotin-peroxidase complex, original magnification × 200). E, Positive staining for CD34 in paracarcinomatous liver tissue (avidin-biotin-peroxidase complex, original magnification × 200). F, Positive staining for CD34 in normal liver tissue (avidin-biotin-peroxidase complex, original magnification × 200).
PMC1475881_F4_5660.jpg
What is the core subject represented in this visual?
TEM of the SPION. A TEM of a sample of the SPION after being pulled through across the RWM model with three cells layers. Shown are clusters of individual SPION particles. Magnification is × 150,000. The scale bar is 20 nm.
PMC1479047_ppat-0020054-g003_5676.jpg
What stands out most in this visual?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5677.jpg
Describe the main subject of this image.
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5680.jpg
What does this image primarily show?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5678.jpg
Describe the main subject of this image.
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5674.jpg
What does this image primarily show?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5671.jpg
What's the most prominent thing you notice in this picture?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5675.jpg
Describe the main subject of this image.
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5670.jpg
What is being portrayed in this visual content?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g003_5673.jpg
What object or scene is depicted here?
Whole-Mount S. mansoni Adult Worm Immunofluorescence S. mansoni adult worms were probed with anti-SmTβRII rabbit IgG and pre-immune rabbit IgG (Figure S2), followed by biotin-conjugated anti-rabbit IgG. Reactive complexes were detected using streptavidin Alexa Fluor 647 conjugate and analyzed with a Bio-Rad MRC1024 confocal laser microscope. Anti-SmTβRII reactivity is shown in a live male worm (♂) (panels A–C) in different laser sections in tubercles (T) (panel B) and gynaecophoric canal (G) (panel C). Specific surface fluorescence is also shown in live female worms (♀) (panels F and I), whereas green fluorescence fields show the non-specific auto-fluorescence in vitellaria (V), oviduct (OvD) (panel E), and ova (Ov) (panels E and H). An acetone-fixed male worm (♂) shows anti-SmTβRII reactivity in the gynaecophoric canal, oral (Os) and ventral suckers (Vs) (panel K), and in esophagus (O) (panel L). Panels A, D, G, and J are phase-contrast fields of the fluorescent fields B and C, E and F, H and I, and K and L, respectively.
PMC1479047_ppat-0020054-g005_5669.jpg
What stands out most in this visual?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5662.jpg
What is the principal component of this image?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5661.jpg
What is the main focus of this visual representation?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5663.jpg
Can you identify the primary element in this image?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5666.jpg
What is being portrayed in this visual content?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5665.jpg
What is the main focus of this visual representation?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479047_ppat-0020054-g005_5664.jpg
What is the main focus of this visual representation?
Localization of SmTβRII mRNA Transcripts in Tissue Sections of S. mansoni Adult Worms by FISHColumn A represent phase-contrast fields, Column B represent non-specific autofluorescence fields observed in vitelline lobules (V) using green fluorescence filter (522 nm) and Column C shows specific probe reactivity as represented by far-red fluorescence using 680 nm filter. Row (I) shows sections of a male (♂) and a female (♀) worm probed with the positive control cRNA probe (the antisense strand of eggshell protein P14). Specific fluorescence could be observed in the vitellaria (V) of the female worm. As expected, no specific fluorescence could be observed in male worm sections. Specific reactivity of SmTβRII antisense probe could be seen in vitelline cells (V) and gut epithelial cells (G) in a female worm section (panel IIC) and in subtegumental cells (STC) in a male worm section (panel IIIC). No significant fluorescence could be seen in the negative control reaction using SmTβRII sense cRNA strand (unpublished data).
PMC1479315_F3_5682.jpg
What is being portrayed in this visual content?
Neuronal GFP expression in the superficial dorsal horn of Pde1c BAC transgenic mice. (A) Overlay showing GFP-immunoreactivity (green) and NeuN-immunoreactivity (red) in spinal cord dorsal horn of a Pde1c BAC transgenic mouse. The insets (bottom left) show higher magnification images of the boxed area shown at right. Arrows show examples of double labeled profiles. (B) Merged images of GFP-immunoreactivity (green) and PKCγ-immunoreactivity (red) in the spinal cord dorsal horn. The insets (bottom left) show higher magnification images of the boxed area. Dotted line illustrates the displacement of the dense band of PKCγ-immunoreactivity from the dorsal aspect in the central portion. (A) and (B) are both montages of low magnification single confocal optical sections. Scale bar, 100 μm in each panel. Dorsal uppermost, medial left both panels.
PMC1479334_F3_5684.jpg
What is the main focus of this visual representation?
Mid-esophageal, 4 chamber, 2D Transoeophageal echocardiogram showing septal plication. IVS = interventricular septum.
PMC1479334_F3_5685.jpg
What is the central feature of this picture?
Mid-esophageal, 4 chamber, 2D Transoeophageal echocardiogram showing septal plication. IVS = interventricular septum.
PMC1479334_F3_5687.jpg
What does this image primarily show?
Mid-esophageal, 4 chamber, 2D Transoeophageal echocardiogram showing septal plication. IVS = interventricular septum.
PMC1479334_F3_5686.jpg
What is the dominant medical problem in this image?
Mid-esophageal, 4 chamber, 2D Transoeophageal echocardiogram showing septal plication. IVS = interventricular septum.
PMC1479342_F1_5689.jpg
What is the main focus of this visual representation?
Pathological lesions in jirds infected with Brugia pahangi. A, Gross pathology of spermatic cord lymphatic vessel of a subcutaneously infected jird with B. pahangi (56 DAI) shows white granulomas (arrows) embedded in the vessel lumen (magnification 3×). B, Lymphatic vessel shows rounded and attached granulomas (arrows) (higher magnification, 7×). C, The same spermatic cord lymphatic vessel at the posterior end also contains a worm (arrows) tucked in the vessel lumen (lymphatic vessel was dyed with Evan's blue for contrast is adjacent to a blood vessel (BV) and embedded in fat tissue (FT), magnification 3×. D, Large nonadherent, florid granulomas from the peritoneal cavity of B. pahangi-infected jirds recovered at the chronic stage (300 DAI), magnification 3×.
PMC1479342_F1_5691.jpg
What is being portrayed in this visual content?
Pathological lesions in jirds infected with Brugia pahangi. A, Gross pathology of spermatic cord lymphatic vessel of a subcutaneously infected jird with B. pahangi (56 DAI) shows white granulomas (arrows) embedded in the vessel lumen (magnification 3×). B, Lymphatic vessel shows rounded and attached granulomas (arrows) (higher magnification, 7×). C, The same spermatic cord lymphatic vessel at the posterior end also contains a worm (arrows) tucked in the vessel lumen (lymphatic vessel was dyed with Evan's blue for contrast is adjacent to a blood vessel (BV) and embedded in fat tissue (FT), magnification 3×. D, Large nonadherent, florid granulomas from the peritoneal cavity of B. pahangi-infected jirds recovered at the chronic stage (300 DAI), magnification 3×.
PMC1479342_F1_5690.jpg
What's the most prominent thing you notice in this picture?
Pathological lesions in jirds infected with Brugia pahangi. A, Gross pathology of spermatic cord lymphatic vessel of a subcutaneously infected jird with B. pahangi (56 DAI) shows white granulomas (arrows) embedded in the vessel lumen (magnification 3×). B, Lymphatic vessel shows rounded and attached granulomas (arrows) (higher magnification, 7×). C, The same spermatic cord lymphatic vessel at the posterior end also contains a worm (arrows) tucked in the vessel lumen (lymphatic vessel was dyed with Evan's blue for contrast is adjacent to a blood vessel (BV) and embedded in fat tissue (FT), magnification 3×. D, Large nonadherent, florid granulomas from the peritoneal cavity of B. pahangi-infected jirds recovered at the chronic stage (300 DAI), magnification 3×.
PMC1479346_F1_5693.jpg
What is the dominant medical problem in this image?
CT scan of the abdomen with contrast media reveals a large-size intra-abdominal mass displacing the adjacent structures. In the same scan a suspicious lesion is identified in the right adrenal. In some rare cases desmoid tumors may co-exist with adrenal or thyroid carcinomas and adrenal adenomas.
PMC1479353_F5_5694.jpg
What is the principal component of this image?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5697.jpg
What stands out most in this visual?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5705.jpg
What stands out most in this visual?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5698.jpg
What is the main focus of this visual representation?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5696.jpg
What is the central feature of this picture?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5700.jpg
What is the central feature of this picture?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5707.jpg
What is the main focus of this visual representation?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5695.jpg
What is the principal component of this image?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5704.jpg
What can you see in this picture?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5699.jpg
What's the most prominent thing you notice in this picture?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5701.jpg
What is the principal component of this image?
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479353_F5_5703.jpg
Describe the main subject of this image.
Immunohistochemistry for Reg3α in wild type and CF mice during acute pancreatitis. Paraffin sections of pancreas from wild type and CF mice, untreated controls or with caerulein-induced pancreatitis, were stained with a Reg3α-specific antibody by the Vectastain ABC technique. Wild type: (A) untreated control; (B) 1 h after a single injection; (C) 7 h after the beginning of seven injections; (D) 3d after the beginning of seven injections. Note reactivity in duct lumina (arrows); and (E) 7d after the beginning of seven injections. CF: (F) untreated control; (G) 1 h; (H) 7 h; (I) 3d; and (J) 7d. Higher magnification images: (K) wild type, 1 h, from the boxed region in panel (B) showing strong expression in a region of inflammation; (L) wild type, 3d, from the boxed region in panel (D) showing expression in a region of fibrosis; (M) untreated CF, from the boxed region in panel (F) showing strong expression in dilated acinar lumina; (N) CF, 1 h, from the boxed region in panel (G) showing expression in acinar cells (arrowhead) and duct lumen (arrow); (O) Omission of the primary antibody (No 1°) as a negative control. This was an adjacent section to that shown in panel (I) focusing on the region of inflammation where specific staining was intense when the primary antibody was included. The scale bar in (J) applies to (A-J). The scale bar in (O) applies to (K-O).
PMC1479356_F3_5712.jpg
What object or scene is depicted here?
Bronchoalveolar lavage showing larval forms of Strongyloides (stained pink) in a background of many hemosiderin laden macrophages (stained blue and marked with solid arrows). Prussian blue stain for iron; 200×. Stains for fungus and Pneumocystis were negative.
PMC1479357_F1_5709.jpg
Describe the main subject of this image.
The H&E sections show a solid area of tumor which consists of cuboidal tumor cells with eosinophilic cytoplasm and vesicular nuclei, with prominent nucleoli. Mitotic figures are frequently observed (a). The immunohistochemical study of the same patient shows faint nuclei staining (intensity 1+) for WT1 protein in the most of tumor cells (b). The immunohistochemical studies show strong immunoreactivity in the nuclei in the majority of tumor cells (c: intensity 2+, d: intensity 3+)
PMC1479357_F1_5711.jpg
What is the dominant medical problem in this image?
The H&E sections show a solid area of tumor which consists of cuboidal tumor cells with eosinophilic cytoplasm and vesicular nuclei, with prominent nucleoli. Mitotic figures are frequently observed (a). The immunohistochemical study of the same patient shows faint nuclei staining (intensity 1+) for WT1 protein in the most of tumor cells (b). The immunohistochemical studies show strong immunoreactivity in the nuclei in the majority of tumor cells (c: intensity 2+, d: intensity 3+)
PMC1479357_F1_5708.jpg
Describe the main subject of this image.
The H&E sections show a solid area of tumor which consists of cuboidal tumor cells with eosinophilic cytoplasm and vesicular nuclei, with prominent nucleoli. Mitotic figures are frequently observed (a). The immunohistochemical study of the same patient shows faint nuclei staining (intensity 1+) for WT1 protein in the most of tumor cells (b). The immunohistochemical studies show strong immunoreactivity in the nuclei in the majority of tumor cells (c: intensity 2+, d: intensity 3+)
PMC1479358_F2_5714.jpg
Describe the main subject of this image.
FABP7 was detected in all grades of astrocytomas. A, lower, expression of FABP7 was analyzed Western blotting using lysate from normal white matter, two GBM specimens (H and L indicate high and low, respectively (high and low FABP7 expression based on the results of the previous microarray study [9]), one oligoastrocytoma grade III (OACIII), and one oligodendroglioma grade III (ODGIII); upper, the protein loading was demonstrated by Coomassie Blue staining. The arrow indicates the size of the FABP7 core protein. The 14 kD band in GBM (L) appeared after longer exposure of the film. The 18 kD band that appeared in the ODGIII lysate may not be non-specific, since several immunoreactive bands in that region could also be detected in lysate from a panel of glioma cell lines (data not shown). Representative photomicrographs of immunohistochemistry of FABP7 in grade II astrocytoma (B), grade III anaplastic astrocytoma (C), and grade I pilocytic astrocytoma (D) demonstrated distinctive FABP7 immunoreactivity in the cytoplasm and cell processes in pilocytic astrocytomas as opposed to the nuclear and cytoplasmic staining in grades II and III astrocytomas. FABP7-positive nuclei that were occasionally detected resembled the Type 1 cells seen in normal brain. The scale of the photomicrographs is the same as in Figure 1 A.
PMC1479358_F2_5713.jpg
What object or scene is depicted here?
FABP7 was detected in all grades of astrocytomas. A, lower, expression of FABP7 was analyzed Western blotting using lysate from normal white matter, two GBM specimens (H and L indicate high and low, respectively (high and low FABP7 expression based on the results of the previous microarray study [9]), one oligoastrocytoma grade III (OACIII), and one oligodendroglioma grade III (ODGIII); upper, the protein loading was demonstrated by Coomassie Blue staining. The arrow indicates the size of the FABP7 core protein. The 14 kD band in GBM (L) appeared after longer exposure of the film. The 18 kD band that appeared in the ODGIII lysate may not be non-specific, since several immunoreactive bands in that region could also be detected in lysate from a panel of glioma cell lines (data not shown). Representative photomicrographs of immunohistochemistry of FABP7 in grade II astrocytoma (B), grade III anaplastic astrocytoma (C), and grade I pilocytic astrocytoma (D) demonstrated distinctive FABP7 immunoreactivity in the cytoplasm and cell processes in pilocytic astrocytomas as opposed to the nuclear and cytoplasmic staining in grades II and III astrocytomas. FABP7-positive nuclei that were occasionally detected resembled the Type 1 cells seen in normal brain. The scale of the photomicrographs is the same as in Figure 1 A.
PMC1479358_F2_5716.jpg
Describe the main subject of this image.
FABP7 was detected in all grades of astrocytomas. A, lower, expression of FABP7 was analyzed Western blotting using lysate from normal white matter, two GBM specimens (H and L indicate high and low, respectively (high and low FABP7 expression based on the results of the previous microarray study [9]), one oligoastrocytoma grade III (OACIII), and one oligodendroglioma grade III (ODGIII); upper, the protein loading was demonstrated by Coomassie Blue staining. The arrow indicates the size of the FABP7 core protein. The 14 kD band in GBM (L) appeared after longer exposure of the film. The 18 kD band that appeared in the ODGIII lysate may not be non-specific, since several immunoreactive bands in that region could also be detected in lysate from a panel of glioma cell lines (data not shown). Representative photomicrographs of immunohistochemistry of FABP7 in grade II astrocytoma (B), grade III anaplastic astrocytoma (C), and grade I pilocytic astrocytoma (D) demonstrated distinctive FABP7 immunoreactivity in the cytoplasm and cell processes in pilocytic astrocytomas as opposed to the nuclear and cytoplasmic staining in grades II and III astrocytomas. FABP7-positive nuclei that were occasionally detected resembled the Type 1 cells seen in normal brain. The scale of the photomicrographs is the same as in Figure 1 A.
PMC1479375_F3_5721.jpg
What is the core subject represented in this visual?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479375_F3_5720.jpg
What's the most prominent thing you notice in this picture?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479375_F3_5722.jpg
What key item or scene is captured in this photo?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479375_F3_5725.jpg
What object or scene is depicted here?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479375_F3_5727.jpg
What is the core subject represented in this visual?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479375_F3_5724.jpg
What can you see in this picture?
Internalization of cell surface receptors in WEHI-231. (A-I) Assay for internalization of BCR, CD40, and TLR4 was performed using WEHI-231 cells. Data from 1 hr stimulations are shown. Line thickness represents treatment of cells following labeling of receptor where thin = acid treated samples and thick = no acid treatment. Black = unstimulated, blue = LPS stimulated, red = AIG stimulated, green = anti-CD40 stimulated. Arrow denotes histogram of acid-treated cells in which labeled BCR is protected from the effects of acid treatment with AIG stimulation. (J) Localization of the BCR by fluorescence microscopy. BCR are labeled with a non-stimulating FITC-conjugated monoclonal anti-IgM antibody (green) and treated for 10 minutes as indicated. DIC images are shown in bottom row.
PMC1479387_pmed-0030215-g006_5728.jpg
What is the core subject represented in this visual?
Rejection of the E42 Pig Pancreas in Different Immunologically Mutant MiceGrafts of E42 pig pancreas were transplanted under the kidney capsule of NOD-SCID (A), C57BL/6 (B), XID (C), and nude (D) mice and harvested 17 d after transplant. Note the extensive fibrosis and infiltration indicating rejection in the C57BL/6 and XID mice, while pancreatic components are seen in the NOD-SCID and nude mice. Each group included five mice.
PMC1479387_pmed-0030215-g006_5731.jpg
What stands out most in this visual?
Rejection of the E42 Pig Pancreas in Different Immunologically Mutant MiceGrafts of E42 pig pancreas were transplanted under the kidney capsule of NOD-SCID (A), C57BL/6 (B), XID (C), and nude (D) mice and harvested 17 d after transplant. Note the extensive fibrosis and infiltration indicating rejection in the C57BL/6 and XID mice, while pancreatic components are seen in the NOD-SCID and nude mice. Each group included five mice.
PMC1479387_pmed-0030215-g006_5730.jpg
What does this image primarily show?
Rejection of the E42 Pig Pancreas in Different Immunologically Mutant MiceGrafts of E42 pig pancreas were transplanted under the kidney capsule of NOD-SCID (A), C57BL/6 (B), XID (C), and nude (D) mice and harvested 17 d after transplant. Note the extensive fibrosis and infiltration indicating rejection in the C57BL/6 and XID mice, while pancreatic components are seen in the NOD-SCID and nude mice. Each group included five mice.
PMC1479387_pmed-0030215-g007_5735.jpg
What can you see in this picture?
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479387_pmed-0030215-g007_5732.jpg
What does this image primarily show?
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479387_pmed-0030215-g007_5737.jpg
Describe the main subject of this image.
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479387_pmed-0030215-g007_5736.jpg
Can you identify the primary element in this image?
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479387_pmed-0030215-g007_5738.jpg
What can you see in this picture?
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479387_pmed-0030215-g007_5733.jpg
What object or scene is depicted here?
Pig Insulin Secretion and Histological Findings of E42 Pig Embryonic Pancreas Transplanted in NOD-SCID and Immunocompetent Mice Treated with Costimulatory Blockade(A) Pig insulin secretion by E42 pig pancreatic grafts implanted under the kidney capsule of NOD-SCID mice (black bars) or C57BL/6 mice treated every 2 wk with CTLA4-Ig and anti-CD40L (grey bars). No pig insulin could be detected in negative control transplanted C57BL/6 mice in the absence of immunosuppression, therefore these results are not shown. Each group included 13 mice.(B) Histological findings 3 mo following E42 pig pancreas transplantation under the kidney capsule of NOD-SCID mice (panel A), immunocompetent C57BL/6 mice (panel B), and C57BL/6 mice treated biweekly with CTAL4-Ig and anti-CD40L (panel C). Note the fierce rejection in C57BL/6 mice evident by implant destruction and fibrosis in (panel B), while intact graft development is demonstrated in C57BL/6 mice treated with CTLA4-Ig and anti-CD40L (panel C) revealing positive staining for insulin (panel D). A small number of mouse CD3 cells (panel E) and macrophages (panel F, stained by F4/80) infiltrated the graft parenchyma (marked by arrowheads) without causing apparent damage to the pancreatic structures.
PMC1479417_pmed-0030233-g002_5741.jpg
What is the focal point of this photograph?
Perfusion-Weighted Imaging (PWI) of the BrainPerfusion amplified signal mismatch (compared to Figure 1), indicating an ischemic area with abnormal function, but no cellular death, salvageable by reperfusion.
PMC1479417_pmed-0030233-g003_5740.jpg
What stands out most in this visual?
Magnetic Resonance Angiography (MRA)MRA shows the left MCA occluded by a thrombus at its origin (white arrow). This technique uses MR to demonstrate blood vessels within the central nervous system.
PMC1479693_pbio-0040215-g004_5747.jpg
What is being portrayed in this visual content?
Reliable Frequency-Selective Regions in the Behaving and in the Anesthetized AnimalsObserved throughout these examples are the two pairs of high and low frequency selective regions as shown in Figure 3, i.e., H1, L1, H2, and L2. (A–C) the results from using two tones. (A–B) show different scanning days with the same anesthetized animal as in Figure 5 (J02, the animal and the experiment are identified in the lower right of each panel). Arrow in (A) shows the axis along which the frequency selective regions lie. (C) Behaving animal results (animal J03). (D–F) Further results with anesthetized animals (J02 and E02). (D) Results using two combinations of tone frequencies (Low: 0.5, 1, and 2 kH tones; High: 4, 8, and 16 kHz tones). (E–F) In two animals these panels reveal the patterns of activity for low and high frequency one-octave band-passed noise (Low: 0.5–1 kHz; High: 4–8 kHz).
PMC1479693_pbio-0040215-g004_5743.jpg
What's the most prominent thing you notice in this picture?
Reliable Frequency-Selective Regions in the Behaving and in the Anesthetized AnimalsObserved throughout these examples are the two pairs of high and low frequency selective regions as shown in Figure 3, i.e., H1, L1, H2, and L2. (A–C) the results from using two tones. (A–B) show different scanning days with the same anesthetized animal as in Figure 5 (J02, the animal and the experiment are identified in the lower right of each panel). Arrow in (A) shows the axis along which the frequency selective regions lie. (C) Behaving animal results (animal J03). (D–F) Further results with anesthetized animals (J02 and E02). (D) Results using two combinations of tone frequencies (Low: 0.5, 1, and 2 kH tones; High: 4, 8, and 16 kHz tones). (E–F) In two animals these panels reveal the patterns of activity for low and high frequency one-octave band-passed noise (Low: 0.5–1 kHz; High: 4–8 kHz).
PMC1479693_pbio-0040215-g004_5745.jpg
What is the principal component of this image?
Reliable Frequency-Selective Regions in the Behaving and in the Anesthetized AnimalsObserved throughout these examples are the two pairs of high and low frequency selective regions as shown in Figure 3, i.e., H1, L1, H2, and L2. (A–C) the results from using two tones. (A–B) show different scanning days with the same anesthetized animal as in Figure 5 (J02, the animal and the experiment are identified in the lower right of each panel). Arrow in (A) shows the axis along which the frequency selective regions lie. (C) Behaving animal results (animal J03). (D–F) Further results with anesthetized animals (J02 and E02). (D) Results using two combinations of tone frequencies (Low: 0.5, 1, and 2 kH tones; High: 4, 8, and 16 kHz tones). (E–F) In two animals these panels reveal the patterns of activity for low and high frequency one-octave band-passed noise (Low: 0.5–1 kHz; High: 4–8 kHz).
PMC1479693_pbio-0040215-g004_5742.jpg
What is the focal point of this photograph?
Reliable Frequency-Selective Regions in the Behaving and in the Anesthetized AnimalsObserved throughout these examples are the two pairs of high and low frequency selective regions as shown in Figure 3, i.e., H1, L1, H2, and L2. (A–C) the results from using two tones. (A–B) show different scanning days with the same anesthetized animal as in Figure 5 (J02, the animal and the experiment are identified in the lower right of each panel). Arrow in (A) shows the axis along which the frequency selective regions lie. (C) Behaving animal results (animal J03). (D–F) Further results with anesthetized animals (J02 and E02). (D) Results using two combinations of tone frequencies (Low: 0.5, 1, and 2 kH tones; High: 4, 8, and 16 kHz tones). (E–F) In two animals these panels reveal the patterns of activity for low and high frequency one-octave band-passed noise (Low: 0.5–1 kHz; High: 4–8 kHz).
PMC1479693_pbio-0040215-g004_5744.jpg
What does this image primarily show?
Reliable Frequency-Selective Regions in the Behaving and in the Anesthetized AnimalsObserved throughout these examples are the two pairs of high and low frequency selective regions as shown in Figure 3, i.e., H1, L1, H2, and L2. (A–C) the results from using two tones. (A–B) show different scanning days with the same anesthetized animal as in Figure 5 (J02, the animal and the experiment are identified in the lower right of each panel). Arrow in (A) shows the axis along which the frequency selective regions lie. (C) Behaving animal results (animal J03). (D–F) Further results with anesthetized animals (J02 and E02). (D) Results using two combinations of tone frequencies (Low: 0.5, 1, and 2 kH tones; High: 4, 8, and 16 kHz tones). (E–F) In two animals these panels reveal the patterns of activity for low and high frequency one-octave band-passed noise (Low: 0.5–1 kHz; High: 4–8 kHz).
PMC1479693_pbio-0040215-g007_5752.jpg
What is the main focus of this visual representation?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5751.jpg
What is the main focus of this visual representation?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5756.jpg
What is the principal component of this image?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5748.jpg
What's the most prominent thing you notice in this picture?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5755.jpg
What is shown in this image?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5749.jpg
What is the central feature of this picture?
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479693_pbio-0040215-g007_5754.jpg
Describe the main subject of this image.
Delineating ACF Borders with Multiple Tones and Bands of Noise(A–C) An additional multi-tone experiment with animal J02. Format as for Figure 5 (A), (C and D). (D–I) Show the results from two animals where five one-octave bands of noise (band-passed noise) were used to localize ACFs. (D and G) Five best noise-band maps showing the antero-posterior frequency gradients. (E and H) show the gradient sign procedure revealing borders between neighboring ACFs. (C, F, and I) show auditory cortex ROI outlines. The results of the gradient tests within these outlines in comparison to the model gradient are shown in Table 1.
PMC1479805_F2_5761.jpg
What is the central feature of this picture?
Coregistration of MRI and PET images. Coregistration of MRI and FDG PET images of a control (top) and an atherosclerotic rabbit (bottom). Each row shows the fused dataset progressing from an anatomical MRI to a sole functional FDG PET image of the same animal in the same location. ROIs of the aorta (indicated with a blue circle and arrow) were identified on the MRI. The software automatically extracted the PET values from the same location in the PET image.
PMC1479805_F2_5762.jpg
What is being portrayed in this visual content?
Coregistration of MRI and PET images. Coregistration of MRI and FDG PET images of a control (top) and an atherosclerotic rabbit (bottom). Each row shows the fused dataset progressing from an anatomical MRI to a sole functional FDG PET image of the same animal in the same location. ROIs of the aorta (indicated with a blue circle and arrow) were identified on the MRI. The software automatically extracted the PET values from the same location in the PET image.
PMC1479805_F3_5758.jpg
What object or scene is depicted here?
In vivo PET images. In vivo PET sagittal (top) and coronal (bottom) images showing the uptake of FDG in the thoracic aorta in a control rabbit (A), in a rabbit with mild atherosclerosis (B).
PMC1479805_F3_5760.jpg
What is the focal point of this photograph?
In vivo PET images. In vivo PET sagittal (top) and coronal (bottom) images showing the uptake of FDG in the thoracic aorta in a control rabbit (A), in a rabbit with mild atherosclerosis (B).
PMC1479808_F1_5763.jpg
What is shown in this image?
CT scan showing leakage of contrast from the left ventricle free wall rupture (arrow).
PMC1479818_F4_5767.jpg
What key item or scene is captured in this photo?
Histological studies of the lungs of immunized mice with different cytokine gene plasmid treatments. Mice that had been sensitized and repeatedly challenged with nebulized saline (A) or OVA (B-E) were gavaged with non-coding vector, scIL-12, TGF-β, or IL-10 gene plasmids before the challenge phase. The data showed extensive cellular infiltration of the peri- airway region from vector DNA treated mice (B). In contrast, lung tissue from scIL-12 plasmid treated mice (C), TGF-β plasmid treated mice (D), and IL-10 plasmid treated mice (E) showed a much less severe inflammation histologically. Microscopic images were made with an Olympus microscope at a magnification of 100, and images were representative of the experimental group. Paraffin embedded sections were stained with hematoxylin and eosin.
PMC1479818_F4_5769.jpg
What is the main focus of this visual representation?
Histological studies of the lungs of immunized mice with different cytokine gene plasmid treatments. Mice that had been sensitized and repeatedly challenged with nebulized saline (A) or OVA (B-E) were gavaged with non-coding vector, scIL-12, TGF-β, or IL-10 gene plasmids before the challenge phase. The data showed extensive cellular infiltration of the peri- airway region from vector DNA treated mice (B). In contrast, lung tissue from scIL-12 plasmid treated mice (C), TGF-β plasmid treated mice (D), and IL-10 plasmid treated mice (E) showed a much less severe inflammation histologically. Microscopic images were made with an Olympus microscope at a magnification of 100, and images were representative of the experimental group. Paraffin embedded sections were stained with hematoxylin and eosin.
PMC1479818_F4_5765.jpg
What is the main focus of this visual representation?
Histological studies of the lungs of immunized mice with different cytokine gene plasmid treatments. Mice that had been sensitized and repeatedly challenged with nebulized saline (A) or OVA (B-E) were gavaged with non-coding vector, scIL-12, TGF-β, or IL-10 gene plasmids before the challenge phase. The data showed extensive cellular infiltration of the peri- airway region from vector DNA treated mice (B). In contrast, lung tissue from scIL-12 plasmid treated mice (C), TGF-β plasmid treated mice (D), and IL-10 plasmid treated mice (E) showed a much less severe inflammation histologically. Microscopic images were made with an Olympus microscope at a magnification of 100, and images were representative of the experimental group. Paraffin embedded sections were stained with hematoxylin and eosin.
PMC1479818_F4_5766.jpg
What object or scene is depicted here?
Histological studies of the lungs of immunized mice with different cytokine gene plasmid treatments. Mice that had been sensitized and repeatedly challenged with nebulized saline (A) or OVA (B-E) were gavaged with non-coding vector, scIL-12, TGF-β, or IL-10 gene plasmids before the challenge phase. The data showed extensive cellular infiltration of the peri- airway region from vector DNA treated mice (B). In contrast, lung tissue from scIL-12 plasmid treated mice (C), TGF-β plasmid treated mice (D), and IL-10 plasmid treated mice (E) showed a much less severe inflammation histologically. Microscopic images were made with an Olympus microscope at a magnification of 100, and images were representative of the experimental group. Paraffin embedded sections were stained with hematoxylin and eosin.
PMC1479836_F8_5791.jpg
What does this image primarily show?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5789.jpg
What stands out most in this visual?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5778.jpg
What is the principal component of this image?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5787.jpg
Describe the main subject of this image.
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5776.jpg
What is the focal point of this photograph?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5785.jpg
What is shown in this image?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5788.jpg
What can you see in this picture?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.
PMC1479836_F8_5777.jpg
What's the most prominent thing you notice in this picture?
Downregulation of survivin by celecoxib and DMC correlates with increased apoptosis in vitro and in vivo. Top half: U87 glioblastoma cells were treated with celecoxib (Cxb) or DMC for 48 hours in vitro; thereafter, cytospins were performed and the cells were subjected to immunohistochemical analysis of survivin protein levels and, in parallel, TUNEL assay for apoptotic cell death. Bottom half: tumor sections from animals described in Figure 7 were analyzed by immunohistochemistry for survivin expression and by TUNEL assay for apoptotic cell death. In all cases, representative sections are shown. Small black rectangles denote enlarged areas of the same photograph shown below. Arrows indicate examples of TUNEL-positive, i.e., apoptotic, cells.