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PMC1508157_F2_6079.jpg | Describe the main subject of this image. | Preoperative CT scan at the level of T8 shows intraspinal dislocation of the left eighth rib head through neural foramen. |
PMC1513216_F4_6081.jpg | What is the core subject represented in this visual? | Tryptase-immunoreactive mast cells in the bronchial biopsy sections from Ltx patients. Mast cell immunolabelling was performed using a mouse monoclonal antibody raised against human tryptase and revealed by FastRed® in lung biopsy specimen from lung transplant recipients (magnitude ×40). |
PMC1513234_F1_6082.jpg | What does this image primarily show? | Example of a pair of corresponding LAO and RAO projections. The three straight lines in each image show corresponding projection lines in the two projections. These lines are used to match the two projections. Numerated squares mark nodes used for segmentation and identification of vascular branches. |
PMC1513234_F1_6083.jpg | Describe the main subject of this image. | Example of a pair of corresponding LAO and RAO projections. The three straight lines in each image show corresponding projection lines in the two projections. These lines are used to match the two projections. Numerated squares mark nodes used for segmentation and identification of vascular branches. |
PMC1513240_F2_6087.jpg | What is shown in this image? | Representative false-color (RGB) autoradiograms of [3H]RX821002 binding density in brain slices from adolescent males and females exposed to either saline or cocaine in utero. Areas of low binding density appear white to light purple, areas of moderate binding appear blue to yellow, and areas of high binding density appear dark red. Measurements were taken from hippocampus (stratum lacunosum-moleculare of CA1 near the hippocampal fissure, as approximately marked by a red oval), parietal cortex (layer II, as approximately marked by an orange rectangle), and amygdala (central nucleus, as approximately marked by a blue square). |
PMC1513240_F2_6086.jpg | What key item or scene is captured in this photo? | Representative false-color (RGB) autoradiograms of [3H]RX821002 binding density in brain slices from adolescent males and females exposed to either saline or cocaine in utero. Areas of low binding density appear white to light purple, areas of moderate binding appear blue to yellow, and areas of high binding density appear dark red. Measurements were taken from hippocampus (stratum lacunosum-moleculare of CA1 near the hippocampal fissure, as approximately marked by a red oval), parietal cortex (layer II, as approximately marked by an orange rectangle), and amygdala (central nucleus, as approximately marked by a blue square). |
PMC1513245_F8_6088.jpg | What is the principal component of this image? | Representative sections of mammaglobin A (SCGB2A2) protein expression as detected by immunohistochemistry. A: Mammaglobin A expression found in invasive ductal carcinoma. B: Mammaglobin A expression in invasive lobular carcinoma. C: Mammaglobin A expression in squamous cell carcinoma of the cervix. D: Mammaglobin A expression in an endometrioid adenocarcinoma of the endometrium. Note that mammaglobin A is not expressed equally in all cells. Magnification: 400 ×. |
PMC1513245_F8_6091.jpg | What object or scene is depicted here? | Representative sections of mammaglobin A (SCGB2A2) protein expression as detected by immunohistochemistry. A: Mammaglobin A expression found in invasive ductal carcinoma. B: Mammaglobin A expression in invasive lobular carcinoma. C: Mammaglobin A expression in squamous cell carcinoma of the cervix. D: Mammaglobin A expression in an endometrioid adenocarcinoma of the endometrium. Note that mammaglobin A is not expressed equally in all cells. Magnification: 400 ×. |
PMC1513334_f1-ehp0114-001083_6096.jpg | What object or scene is depicted here? | DCs express IP3R and RyR Ca2+ channels. Abbreviations: Ab, antibody; mAb, monoclonal antibody. (A–D) IDCs labeled with anti-IP3R1 (A) or anti-RyR (C) or with anti-IP3R1 plus blocking peptide (B) or fluorescent second-step Ab alone (D). Nuclei were counterstained with 7AAD; magnification, 100×. (E and F) Merged images of RyR1 and IP3R (pan anti-IP3R) immunostaining in IDCs (E), and RyR1 and 7AAD nuclear staining (F); images were acquired at 100× with 2.5× digital magnification. (G and H) IDCs (G) and MDCs (H) stained for RyR; magnification, 40×. Bars = 10 μm. |
PMC1513334_f1-ehp0114-001083_6095.jpg | What is the main focus of this visual representation? | DCs express IP3R and RyR Ca2+ channels. Abbreviations: Ab, antibody; mAb, monoclonal antibody. (A–D) IDCs labeled with anti-IP3R1 (A) or anti-RyR (C) or with anti-IP3R1 plus blocking peptide (B) or fluorescent second-step Ab alone (D). Nuclei were counterstained with 7AAD; magnification, 100×. (E and F) Merged images of RyR1 and IP3R (pan anti-IP3R) immunostaining in IDCs (E), and RyR1 and 7AAD nuclear staining (F); images were acquired at 100× with 2.5× digital magnification. (G and H) IDCs (G) and MDCs (H) stained for RyR; magnification, 40×. Bars = 10 μm. |
PMC1513334_f1-ehp0114-001083_6093.jpg | What is the central feature of this picture? | DCs express IP3R and RyR Ca2+ channels. Abbreviations: Ab, antibody; mAb, monoclonal antibody. (A–D) IDCs labeled with anti-IP3R1 (A) or anti-RyR (C) or with anti-IP3R1 plus blocking peptide (B) or fluorescent second-step Ab alone (D). Nuclei were counterstained with 7AAD; magnification, 100×. (E and F) Merged images of RyR1 and IP3R (pan anti-IP3R) immunostaining in IDCs (E), and RyR1 and 7AAD nuclear staining (F); images were acquired at 100× with 2.5× digital magnification. (G and H) IDCs (G) and MDCs (H) stained for RyR; magnification, 40×. Bars = 10 μm. |
PMC1513334_f1-ehp0114-001083_6098.jpg | What does this image primarily show? | DCs express IP3R and RyR Ca2+ channels. Abbreviations: Ab, antibody; mAb, monoclonal antibody. (A–D) IDCs labeled with anti-IP3R1 (A) or anti-RyR (C) or with anti-IP3R1 plus blocking peptide (B) or fluorescent second-step Ab alone (D). Nuclei were counterstained with 7AAD; magnification, 100×. (E and F) Merged images of RyR1 and IP3R (pan anti-IP3R) immunostaining in IDCs (E), and RyR1 and 7AAD nuclear staining (F); images were acquired at 100× with 2.5× digital magnification. (G and H) IDCs (G) and MDCs (H) stained for RyR; magnification, 40×. Bars = 10 μm. |
PMC1513394_F1_6104.jpg | What stands out most in this visual? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6107.jpg | What is being portrayed in this visual content? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6105.jpg | What is shown in this image? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6103.jpg | What is shown in this image? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6106.jpg | What is the principal component of this image? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6102.jpg | What is being portrayed in this visual content? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6100.jpg | What can you see in this picture? | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513394_F1_6101.jpg | Describe the main subject of this image. | Histochemical staining of frozen sections of an intraperitoneal primary mouse plasma cell tumor. A nodule rich in plasma cells can be seen in the midst of surrounding stromal and fat cells. The stains of the sections are as follows: H&E, hematoxylin and eosin; Nissl; MGP (methyl green pyronin) and kappa (immunoperoxidase in the top four panels or immunofluorescence in the bottom four panels) staining of immunoglobulin kappa light chains). The images in the top four panels represent frozen sections stained and mounted under cover slips in a distant histology laboratory and captured using high quality optics in a photographic studio. Those in the bottom four panels reflect the challenges of interpreting microscopic views on a microscope optimized for LCM, viewing frozen sections that had been processed and stained with the utmost speed to minimize RNA damage and lacking cover slips. Ovals indicate clusters of plasma cells with characteristic MGP staining and rich in Ig κ protein. |
PMC1513524_F3_6111.jpg | What can you see in this picture? | Posteroanterior chest x-ray film. Top (A): radiograph obtained 24 hours after pacemaker implantation. Arrowhead shows atrial lead tip inside the right atrial appendage. Bottom (B): radiograph obtained three months later, showing displacement of atrial lead towards tricuspid annulus. However, diagnosis is not evident from the posteroanterior view alone, due to superimposition of structures and radiograph densities. |
PMC1513524_F3_6110.jpg | What's the most prominent thing you notice in this picture? | Posteroanterior chest x-ray film. Top (A): radiograph obtained 24 hours after pacemaker implantation. Arrowhead shows atrial lead tip inside the right atrial appendage. Bottom (B): radiograph obtained three months later, showing displacement of atrial lead towards tricuspid annulus. However, diagnosis is not evident from the posteroanterior view alone, due to superimposition of structures and radiograph densities. |
PMC1513524_F4_6108.jpg | What is the central feature of this picture? | Lateral chest x-ray film (same patient as in figure 3): left (A): 24 hours post implantation. Right (B): 3 months later. Atrial lead displacement diagnosis is now straightforward. The example emphasizes the relevance of two chest views available to correctly evaluate lead displacement. |
PMC1513524_F4_6109.jpg | What is the core subject represented in this visual? | Lateral chest x-ray film (same patient as in figure 3): left (A): 24 hours post implantation. Right (B): 3 months later. Atrial lead displacement diagnosis is now straightforward. The example emphasizes the relevance of two chest views available to correctly evaluate lead displacement. |
PMC1513552_F3_6118.jpg | Describe the main subject of this image. | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6116.jpg | What is the principal component of this image? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6112.jpg | What object or scene is depicted here? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6115.jpg | What is the central feature of this picture? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6114.jpg | What can you see in this picture? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6119.jpg | What does this image primarily show? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F3_6113.jpg | What is the core subject represented in this visual? | Ovarian localization of bovine MATER protein by immunohistochemistry. Adjacent ovarian sections showing primary follicle (A, E), pre-antral follicle (B, F), early antral follicle (C, G) and over 1 mm antral follicle (D, H) incubated with either anti-MATER serum (A-D) or preimmune serum (E-H) followed by biotinylated horse anti-rabbit IgG antibody. Scale bar: 100 μm. Inset in A is the same oocyte shown at higher magnification. |
PMC1513552_F5_6127.jpg | What is the core subject represented in this visual? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513552_F5_6122.jpg | What object or scene is depicted here? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513552_F5_6124.jpg | What stands out most in this visual? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513552_F5_6126.jpg | What is the main focus of this visual representation? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513552_F5_6129.jpg | What's the most prominent thing you notice in this picture? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513552_F5_6121.jpg | What is the main focus of this visual representation? | Localization of MATER protein in bovine oocytes and preimplantation embryos by confocal microscopy. Oocytes and embryos were incubated with anti-MATER peptide purified antibody (1:250) and revealed by green staining. Scale bar: 50 μm. (A) Oocytes at GV or NEBD stage. Lamins A/C are stained in red (middle row). Chromatin is stained in blue with Hoechst 33258 (lower row). (B) immature oocytes (IO), in vitro matured oocytes (MO), in vitro cultured zygote (1C), 2-, 4-, 8- cell embryos, morula (mo), expanded blastocysts (EB) and hatched blastocysts (HB). |
PMC1513554_F1_6131.jpg | What does this image primarily show? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6135.jpg | Can you identify the primary element in this image? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6139.jpg | Describe the main subject of this image. | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6132.jpg | What is the dominant medical problem in this image? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6134.jpg | What's the most prominent thing you notice in this picture? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6133.jpg | What object or scene is depicted here? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6138.jpg | What is the principal component of this image? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6141.jpg | Can you identify the primary element in this image? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6136.jpg | Describe the main subject of this image. | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513554_F1_6140.jpg | What is shown in this image? | Dictyostelium wild-type Ax2 cells expressing dajumin-GFP or calnexin-GFP. (A): The contractile vacuole system of a Dictyostelium cell is visualized by expression of dajumin-GFP using live cell confocal microscopy (upper panels). Dynamics of the bladder is indicated by formation of irregular ventricles and ducts. Lower panels show the corresponding bright field images. (B): A Dictyostelium cell expressing calnexin-GFP to visualize the endoplasmic reticulum is shown in the upper panels. Note that the perinuclear region is intensively labeled as observed previously for calnexin-GFP and antibody-stained preparations, whereas a vacuole in the left part of the cell is devoid of any label. Time of depicted frames is indicated in seconds. Bar corresponds to 10 μm. |
PMC1513558_F2_6144.jpg | What is the focal point of this photograph? | PrPSc accumulation in the pyloric caeca (a, b, c) and non-everted intestine (d, e, f) of trout intestine statically perfused. Immunohistochemistry on trout sections of pyloric caeca (a, b, c) and non-everted intestines (d, e, f). The static perfusion was performed for 1 hour at 15°C, in a PBS solution containing 50 μl/ml of either 10% uninfected mice brain homogenate (a, d) or 10% mice scrapie brain homogenate (139A) (b, c, e, f). Immunolabelling was performed with the monoclonal antibody SAF83 (a, c, d, f), or, as control, the monoclonal anti-HA against influenza virus (anti-HA, clone 12CA5, subtype IgG2b, k), (b, e). All the immunogold-labelled sections were silver enhanced and counterstained with 0.1% toluidine blue (see materials and methods for details). PrPSc localisation (arrows) occurred in the stratum compactum (S) of distal intestine and pyloric caecum incubated with SAF83 (c, f). No immunolabelling was present in control tissues incubated with the same antibody (a, d), except for a low unspecific background. The background was slightly higher, though unspecific, in anti-HA labelled sections (b, e) due to the high reactivity of this antibody. All micrographs were taken at the same magnifications; V = villo, T = tunica muscolaris; asterisks point to unspecific labelling present in all samples and due to micro-fractures between the outer specimen surface and the resin. |
PMC1513558_F2_6145.jpg | Can you identify the primary element in this image? | PrPSc accumulation in the pyloric caeca (a, b, c) and non-everted intestine (d, e, f) of trout intestine statically perfused. Immunohistochemistry on trout sections of pyloric caeca (a, b, c) and non-everted intestines (d, e, f). The static perfusion was performed for 1 hour at 15°C, in a PBS solution containing 50 μl/ml of either 10% uninfected mice brain homogenate (a, d) or 10% mice scrapie brain homogenate (139A) (b, c, e, f). Immunolabelling was performed with the monoclonal antibody SAF83 (a, c, d, f), or, as control, the monoclonal anti-HA against influenza virus (anti-HA, clone 12CA5, subtype IgG2b, k), (b, e). All the immunogold-labelled sections were silver enhanced and counterstained with 0.1% toluidine blue (see materials and methods for details). PrPSc localisation (arrows) occurred in the stratum compactum (S) of distal intestine and pyloric caecum incubated with SAF83 (c, f). No immunolabelling was present in control tissues incubated with the same antibody (a, d), except for a low unspecific background. The background was slightly higher, though unspecific, in anti-HA labelled sections (b, e) due to the high reactivity of this antibody. All micrographs were taken at the same magnifications; V = villo, T = tunica muscolaris; asterisks point to unspecific labelling present in all samples and due to micro-fractures between the outer specimen surface and the resin. |
PMC1513558_F2_6143.jpg | Can you identify the primary element in this image? | PrPSc accumulation in the pyloric caeca (a, b, c) and non-everted intestine (d, e, f) of trout intestine statically perfused. Immunohistochemistry on trout sections of pyloric caeca (a, b, c) and non-everted intestines (d, e, f). The static perfusion was performed for 1 hour at 15°C, in a PBS solution containing 50 μl/ml of either 10% uninfected mice brain homogenate (a, d) or 10% mice scrapie brain homogenate (139A) (b, c, e, f). Immunolabelling was performed with the monoclonal antibody SAF83 (a, c, d, f), or, as control, the monoclonal anti-HA against influenza virus (anti-HA, clone 12CA5, subtype IgG2b, k), (b, e). All the immunogold-labelled sections were silver enhanced and counterstained with 0.1% toluidine blue (see materials and methods for details). PrPSc localisation (arrows) occurred in the stratum compactum (S) of distal intestine and pyloric caecum incubated with SAF83 (c, f). No immunolabelling was present in control tissues incubated with the same antibody (a, d), except for a low unspecific background. The background was slightly higher, though unspecific, in anti-HA labelled sections (b, e) due to the high reactivity of this antibody. All micrographs were taken at the same magnifications; V = villo, T = tunica muscolaris; asterisks point to unspecific labelling present in all samples and due to micro-fractures between the outer specimen surface and the resin. |
PMC1513558_F2_6147.jpg | What is the dominant medical problem in this image? | PrPSc accumulation in the pyloric caeca (a, b, c) and non-everted intestine (d, e, f) of trout intestine statically perfused. Immunohistochemistry on trout sections of pyloric caeca (a, b, c) and non-everted intestines (d, e, f). The static perfusion was performed for 1 hour at 15°C, in a PBS solution containing 50 μl/ml of either 10% uninfected mice brain homogenate (a, d) or 10% mice scrapie brain homogenate (139A) (b, c, e, f). Immunolabelling was performed with the monoclonal antibody SAF83 (a, c, d, f), or, as control, the monoclonal anti-HA against influenza virus (anti-HA, clone 12CA5, subtype IgG2b, k), (b, e). All the immunogold-labelled sections were silver enhanced and counterstained with 0.1% toluidine blue (see materials and methods for details). PrPSc localisation (arrows) occurred in the stratum compactum (S) of distal intestine and pyloric caecum incubated with SAF83 (c, f). No immunolabelling was present in control tissues incubated with the same antibody (a, d), except for a low unspecific background. The background was slightly higher, though unspecific, in anti-HA labelled sections (b, e) due to the high reactivity of this antibody. All micrographs were taken at the same magnifications; V = villo, T = tunica muscolaris; asterisks point to unspecific labelling present in all samples and due to micro-fractures between the outer specimen surface and the resin. |
PMC1513580_F5_6154.jpg | What is the main focus of this visual representation? | Immunohistochemical localization of CRH-R1 and CRH-R2 in the human cervix. CRH-R1 in the cervical epithelium at (A) non-pregnant state, (B) term not in labor, (C) term in labor. CRH-R2 in the (A) cervical epithelium at non-pregnant state, (B) cervical epithelium and extracellular matrix at term not in labor cervix, (C) extracellular matrix at term in labor cervix. These samples were fixed in a 4% formaldehyde solution for a maximum of 24 hours and subsequently dehydrated in 70% ethanol. The brown color represents positive staining. The magnification is 1 × 200. |
PMC1513580_F5_6157.jpg | What is the main focus of this visual representation? | Immunohistochemical localization of CRH-R1 and CRH-R2 in the human cervix. CRH-R1 in the cervical epithelium at (A) non-pregnant state, (B) term not in labor, (C) term in labor. CRH-R2 in the (A) cervical epithelium at non-pregnant state, (B) cervical epithelium and extracellular matrix at term not in labor cervix, (C) extracellular matrix at term in labor cervix. These samples were fixed in a 4% formaldehyde solution for a maximum of 24 hours and subsequently dehydrated in 70% ethanol. The brown color represents positive staining. The magnification is 1 × 200. |
PMC1513580_F5_6158.jpg | What is the main focus of this visual representation? | Immunohistochemical localization of CRH-R1 and CRH-R2 in the human cervix. CRH-R1 in the cervical epithelium at (A) non-pregnant state, (B) term not in labor, (C) term in labor. CRH-R2 in the (A) cervical epithelium at non-pregnant state, (B) cervical epithelium and extracellular matrix at term not in labor cervix, (C) extracellular matrix at term in labor cervix. These samples were fixed in a 4% formaldehyde solution for a maximum of 24 hours and subsequently dehydrated in 70% ethanol. The brown color represents positive staining. The magnification is 1 × 200. |
PMC1513580_F5_6153.jpg | What can you see in this picture? | Immunohistochemical localization of CRH-R1 and CRH-R2 in the human cervix. CRH-R1 in the cervical epithelium at (A) non-pregnant state, (B) term not in labor, (C) term in labor. CRH-R2 in the (A) cervical epithelium at non-pregnant state, (B) cervical epithelium and extracellular matrix at term not in labor cervix, (C) extracellular matrix at term in labor cervix. These samples were fixed in a 4% formaldehyde solution for a maximum of 24 hours and subsequently dehydrated in 70% ethanol. The brown color represents positive staining. The magnification is 1 × 200. |
PMC1513580_F5_6156.jpg | What is the central feature of this picture? | Immunohistochemical localization of CRH-R1 and CRH-R2 in the human cervix. CRH-R1 in the cervical epithelium at (A) non-pregnant state, (B) term not in labor, (C) term in labor. CRH-R2 in the (A) cervical epithelium at non-pregnant state, (B) cervical epithelium and extracellular matrix at term not in labor cervix, (C) extracellular matrix at term in labor cervix. These samples were fixed in a 4% formaldehyde solution for a maximum of 24 hours and subsequently dehydrated in 70% ethanol. The brown color represents positive staining. The magnification is 1 × 200. |
PMC1513580_F6_6151.jpg | What is being portrayed in this visual content? | Negative controls for immunohistochemical staining of the CRH, CRH-BP, CRH-R1 and CRH-R2 in the cervix. Negative controls for the staining of (A) CRH, (B) CRH-BP, (C) CRH-R1 and (D) CRH-R2. The magnification is 1 × 100 in (A) and (B), 1 × 200 in (C) and (D). |
PMC1513580_F6_6149.jpg | What can you see in this picture? | Negative controls for immunohistochemical staining of the CRH, CRH-BP, CRH-R1 and CRH-R2 in the cervix. Negative controls for the staining of (A) CRH, (B) CRH-BP, (C) CRH-R1 and (D) CRH-R2. The magnification is 1 × 100 in (A) and (B), 1 × 200 in (C) and (D). |
PMC1513580_F6_6150.jpg | What is the focal point of this photograph? | Negative controls for immunohistochemical staining of the CRH, CRH-BP, CRH-R1 and CRH-R2 in the cervix. Negative controls for the staining of (A) CRH, (B) CRH-BP, (C) CRH-R1 and (D) CRH-R2. The magnification is 1 × 100 in (A) and (B), 1 × 200 in (C) and (D). |
PMC1513580_F6_6152.jpg | What is the core subject represented in this visual? | Negative controls for immunohistochemical staining of the CRH, CRH-BP, CRH-R1 and CRH-R2 in the cervix. Negative controls for the staining of (A) CRH, (B) CRH-BP, (C) CRH-R1 and (D) CRH-R2. The magnification is 1 × 100 in (A) and (B), 1 × 200 in (C) and (D). |
PMC1513581_F2_6160.jpg | What does this image primarily show? | (a) Under higher magnification, the microsporidal spores are seen as pink oval structures (H & E stain, × 1000); (b) magenta pink oval structures in PAS stain (× 500), (c) deep blue oval structures with dark tip (arrow) in some spores Giemsa stain (× 500), (d) well defined brown oval spores with dark tip or band in Gomoris methenamine silver stain (× 500) |
PMC1513581_F2_6159.jpg | What is the main focus of this visual representation? | (a) Under higher magnification, the microsporidal spores are seen as pink oval structures (H & E stain, × 1000); (b) magenta pink oval structures in PAS stain (× 500), (c) deep blue oval structures with dark tip (arrow) in some spores Giemsa stain (× 500), (d) well defined brown oval spores with dark tip or band in Gomoris methenamine silver stain (× 500) |
PMC1513591_F4_6165.jpg | What is the main focus of this visual representation? | CT scan of abdomen showing liver metastases. |
PMC1513593_F1_6166.jpg | What is the principal component of this image? | Computerized tomography shows a heterogeneous mass originating from the lower pole of the left kidney and infiltrating to the psoas muscle. |
PMC1513593_F1_6167.jpg | What object or scene is depicted here? | Computerized tomography shows a heterogeneous mass originating from the lower pole of the left kidney and infiltrating to the psoas muscle. |
PMC1513595_F1_6168.jpg | What does this image primarily show? | MRI images of patients in the sagittal plane. MRI image showing complete agenesis of the corpus callosum and the preserved anterior commissure of (A) patient M.G. and (B) patient S.G., as well as complete agenesis of both the corpus callosum and the anterior commissure of (C) patient S.Pe. |
PMC1513595_F1_6170.jpg | What is the principal component of this image? | MRI images of patients in the sagittal plane. MRI image showing complete agenesis of the corpus callosum and the preserved anterior commissure of (A) patient M.G. and (B) patient S.G., as well as complete agenesis of both the corpus callosum and the anterior commissure of (C) patient S.Pe. |
PMC1514789_pbio-0040289-g001_6172.jpg | Describe the main subject of this image. | Electron micrographs of rapidly fixed worm synapses reveal differences in synaptic vesicle localization in tomosyn mutants versus wild type. |
PMC1514789_pbio-0040289-g001_6173.jpg | What is being portrayed in this visual content? | Electron micrographs of rapidly fixed worm synapses reveal differences in synaptic vesicle localization in tomosyn mutants versus wild type. |
PMC1523192_F2_6174.jpg | Describe the main subject of this image. | MRI of SFT. MRI of a solitary fibrous tumor depicting a relatively well circumscribed soft tissue tumor in the forearm. |
PMC1523192_F4_6175.jpg | What is the core subject represented in this visual? | Microscopic aspect of SFT (a). Microscopic aspect of a solitary fibrous tumor: "Patternless" growth pattern with cellular (right half) and some myxoid (left half) areas, hemangiopericytoma-like wide blood vessels. |
PMC1523192_F5_6176.jpg | What is being portrayed in this visual content? | Microscopic aspect of SFT (b). Microscopic aspect of a solitary fibrous tumor, higher magnification: fibroblastic, partly "neural-like" tumor cells lying in a fibrous, partly hyalinized matrix, hemangiopericytoma-like blood vessels. |
PMC1523201_F3_6191.jpg | What is the dominant medical problem in this image? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6196.jpg | What key item or scene is captured in this photo? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6189.jpg | What key item or scene is captured in this photo? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6192.jpg | What is the main focus of this visual representation? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6190.jpg | What can you see in this picture? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6198.jpg | What is being portrayed in this visual content? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6197.jpg | What is the core subject represented in this visual? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F3_6193.jpg | What is the principal component of this image? | Expression pattern for the wdr68 gene during early development. A) Transcripts are present in all cells of the animal at sphere stage. B) Ubiquitous expression at tailbud stage. C) Ubiquitous expression at 5 somites stage. D) Ubiquitous expression at 10 somites stage. E) Ubiquitous expression with modest enrichment in the forebrain and hindbrain at 20 somites stage. Also note emerging patterned expression in the developing somites. F) Dorsal view of same 20 somites stage animal indicating ubiquitous expression. G) Ubiquitous expression with moderate enrichment in the head region at 24 hpf. H) Higher magnification image of a 28 hpf animal to show patterned expression in somites. I, J) Wild type sibling and dys/dys homozygous mutant at 34 hpf. I) Enriched expression in the head region with much lower expression in somites in a 34 hpf wild type sibling animal. J) Absence of detectable wdr68 transcripts in the dys/dys homozygous mutant sibling. K) Dorsal view of head region of 34 hpf animal showing near ubiquitous expression in developing head structures. |
PMC1523201_F7_6187.jpg | What key item or scene is captured in this photo? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6185.jpg | What is the main focus of this visual representation? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6178.jpg | What is the core subject represented in this visual? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6180.jpg | What is the core subject represented in this visual? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6179.jpg | What key item or scene is captured in this photo? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6188.jpg | What's the most prominent thing you notice in this picture? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6184.jpg | What can you see in this picture? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6186.jpg | Describe the main subject of this image. | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6181.jpg | What is the core subject represented in this visual? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6177.jpg | What is being portrayed in this visual content? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6183.jpg | What is the principal component of this image? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523201_F7_6182.jpg | Can you identify the primary element in this image? | The Wdr68 protein co-localizes with Dyrk1a. Transiently transfected HEK-293FT cells. A, E, I) Fluorescence of GFP and fusion proteins. B, F, J) Fluorescence of mRFP1 and fusion proteins. C, G, K) Fluorescence of nuclei stained with DAPI. D, H, L) Composite overlay image of GFP, RFP and DAPI signals. A, B, C, D) GFP and mRFP1 distribute throughout the cytoplasm and nucleus. E, F, G, H) The GFP-Wdr68 fusion and the mRFP1-Dyrk1a fusion are co-localized to the nucleus. I, J, K, L) The GFP-T284F Wdr68 mutant does not co-localize with nuclear mRFP1-Dyrk1a. |
PMC1523202_F1_6201.jpg | What is the central feature of this picture? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F1_6203.jpg | What is the focal point of this photograph? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F1_6207.jpg | What object or scene is depicted here? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F1_6204.jpg | What can you see in this picture? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F1_6205.jpg | What is the focal point of this photograph? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F1_6200.jpg | What can you see in this picture? | Cadherin2 expression in developing zebrafish heart. Anterior is to the left and dorsal is up for panels A-H. Panels A-C are lateral views of the head region of whole mount zebrafish embryos labeled by in situ hybridization with cadherin-2 cRNA. The arrow points to the heart. Panels D and E are lateral views of higher magnifications of the heart. Panel F is a parasagittal section of a heart processed for whole mount in situ hybridization. Panels G and H are parasagittal sections of the ventricle processed for cadherin-2 (Cdh2) and Zn-5 immunocytochemical staining, respectively, both showing that the staining is confined mainly to cell membranes of myocardiocytes. Panels I and J show the same cross section of the ventricle (dorsal up) double-labeled with cadherin-2 antibody (panel I) and Zn-5 antibody (panel J). The arrows and arrowheads point to the same cells respectively. Abbreviations: a, atrium; ba, bulbus arteriosus; c, cerebellum; di, diencephalon; ec, endothelium; h, hindbrain; mc, myocardium; ot, optic tectum; v, ventricle. |
PMC1523202_F3_6209.jpg | What is the focal point of this photograph? | Enlarged pericardial cavity in glo mutant. Compared to a control embryo (panel A), the pericardial cavity (pc) is much enlarged in a cadherin2 morphant (panel B) and a glo mutant embryo (panels C and D). All panels are images from live embryos showing lateral views (anterior to the left and dorsal up) of the pericardial cavity and heart. Panel D is a higher magnification of the pericardial cavity showing in panel C. |
PMC1523202_F3_6210.jpg | What object or scene is depicted here? | Enlarged pericardial cavity in glo mutant. Compared to a control embryo (panel A), the pericardial cavity (pc) is much enlarged in a cadherin2 morphant (panel B) and a glo mutant embryo (panels C and D). All panels are images from live embryos showing lateral views (anterior to the left and dorsal up) of the pericardial cavity and heart. Panel D is a higher magnification of the pericardial cavity showing in panel C. |
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